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4 results on '"Alain Sylvin Bell"'

1. NOTCH1 PEST domain mutation is an adverse prognostic factor in B-CLL

Author

Barbara Crescenzi, Emanuela Rosati, Stefano Baldoni, Raffaella Ciurnelli, Massimo F. Martelli, Cristina Mecucci, Franca Falzetti, Paolo Sportoletti, Ambra Di Tommaso, Laura Cavalli, Beatrice Del Papa, Pierfrancesco Marconi, Alain Sylvin Bell, Isabella Screpanti, Mauro Di Ianni, and Elisabetta Bonifacio

Subjects
Prognostic factor, medicine.medical_specialty, Hematology, Chronic lymphocytic leukemia, Cancer, Biology, medicine.disease, Complement factor B, Internal medicine, Immunology, Mutation (genetic algorithm), Cancer research, medicine, PEST analysis
Published
2010
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2. NOTCH1 PEST domain mutation is an adverse prognostic factor in B-CLL

Author

Paolo, Sportoletti, Stefano, Baldoni, Laura, Cavalli, Beatrice Del Papa, Elisabetta, Bonifacio, Raffaella, Ciurnelli, Alain Sylvin Bell, Ambra Di Tommaso, Emanuela, Rosati, Barbara, Crescenzi, Cristina, Mecucci, Screpanti, Isabella, Pierfrancesco, Marconi, Martelli, Massimo F., Mauro Di Ianni, and Franca, Falzetti

Subjects
Mutation, Humans, leukaemia, mutations, prognostic factors, Receptor, Notch1, Prognosis, Leukemia, Lymphocytic, Chronic, B-Cell
Published
2010

3. A microelectronic DNA chip detects the V617F JAK-2 mutation in myeloproliferative disorders

Author

M Di Ianni, Lorenzo Moretti, Alain Sylvin Bell, Antonio Tabilio, E Gaozza, Franca Falzetti, and B. Del Papa

Subjects
Cancer Research, Janus kinase 2, Myeloproliferative Disorders, biology, Point mutation, DNA Mutational Analysis, Hematology, Janus Kinase 2, Protein-Tyrosine Kinases, Molecular biology, Oncology, hemic and lymphatic diseases, Proto-Oncogene Proteins, Mutation (genetic algorithm), biology.protein, Humans, Point Mutation, DNA microarray, Oligonucleotide Array Sequence Analysis
Abstract

A microelectronic DNA chip detects the V617F JAK-2 mutation in myeloproliferative disorders

Published
2006

4. Interleukin7 engineered mesenchymal cells: in vitro effects on naive T cell population

Author

Lorenzo Moretti, Elisabetta Bonifacio, Massimo F. Martelli, Paolo Sportoletti, Katia Fettucciari, Mauro Di Ianni, Mariangela De Ioanni, Antonio Tabilio, Vania Lanterna, Tiziana Zei, Alain Sylvin Bell, Beatrice Del Papa, Eugenia Carnevali, and Franca Falzetti

Subjects
Receptors, CCR7, CD45RA+, Naive T cell, medicine.medical_treatment, T cell, proliferation, Recombinant Fusion Proteins, Population, Genetic Vectors, C-C chemokine receptor type 7, Apoptosis, Biology, Mesenchymal Stem Cell Transplantation, survival, Immunophenotyping, S Phase, T-cell, Antigens, CD, T-Lymphocyte Subsets, Transduction, Genetic, interleukin (IL)-7, medicine, Humans, IL-2 receptor, Gene Rearrangement, beta-Chain T-Cell Antigen Receptor, education, Cells, Cultured, education.field_of_study, Transplantation, Dose-Response Relationship, Drug, Mesenchymal stromal cell, Interleukin-7, Mesenchymal stem cell, Interleukin, Mesenchymal Stem Cells, Hematology, mesenchymal stromal cells (MSCs), Molecular biology, Coculture Techniques, Cytokine, medicine.anatomical_structure, Retroviridae, Immunology, Receptors, Chemokine, Genetic Engineering
Abstract

T-cell homeostasis is regulated by several molecules; among these, interleukin (IL)-7 plays an essential role in the survival and homeostatic proliferation of peripheral naive T cells. In a previous study, we investigated whether human mesenchymal stromal cells (MSCs) could be engineered with the IL-7 gene to produce functional level of this cytokine. In the present study, we analyzed the impact of different quantities of IL-7 produced by MSCs on the survival and proliferation of a negative immunoselected naive (CD3(+)/CD45RA(+)) T-cell population. Co-cultivation of peripheral naive T cells with MSCs producing low (16 pg/mL) or high (1000 pg/mL) IL-7 levels or in the presence of exogenous IL-7 (0.01 ng/mL and 100 ng/mL) maintained the CD3(+)/CD45RA(+) naive T-cell phenotype. Chemokine receptor CCR7(+) expression was also maintained among this T-cell population. Naive T-cell molecular characteristics were maintained as assessed by the Vbeta spectratyping complexity score, which showed the maintenance of a broad T-cell repertoire. No Th1 or Th2 differentiation was observed, as assessed by interferon-gamma or IL-4 accumulation. In contrast, only MSCs producing high amounts of IL-7 caused increased activation (CD25 31.2% +/- 12% vs 10% +/- 3.5%; P.05), proliferation (CD71 17.8+/-7% vs 9.3%+/-3, P.05), apoptosis (assessed by annexin V: 18.6% +/- 5% vs 14.9% +/- 2.6%; P.05), and the phase S cell cycle (15% vs 6.9%, P.05). Exogenous IL-7 exhibited no significant effect. In conclusion, we demonstrated that IL-7 produced by MSCs has a dose-independent effect on naive T-cell survival while exerting a dose-dependent effect on activation/proliferation. Due to the continuous production of IL-7 by engineered cells, our system is more efficacious than exogenous IL-7.

Published
2006

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