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143 results on '"Amorim, Mónica J.B."'

32. Molecular mechanisms of zinc toxicity in the potworm Enchytraeus crypticus, analysed by high-throughput gene expression profiling

Author

Gomes, Susana I.L., de Boer, Tjalf E., van Gestel, Cornelis A.M., van Straalen, Nico M., Soares, Amadeu M.V.M., Roelofs, Dick, Amorim, Mónica J.B., Gomes, Susana I.L., de Boer, Tjalf E., van Gestel, Cornelis A.M., van Straalen, Nico M., Soares, Amadeu M.V.M., Roelofs, Dick, and Amorim, Mónica J.B.

Abstract

Zinc (Zn) is known to be relatively toxic to some soil-living invertebrates including the ecologically important enchytraeid worms. To reveal the molecular mechanisms of zinc toxicity we assessed the gene expression profile of Enchytraeus crypticus (Enchytraeidae), exposed to the reproduction effect concentrations EC10 and EC50, over 4 consecutive days, using a high-throughput microarray (species customized). Three main mechanisms of toxicity to Zn were observed: 1) Zn trafficking (upregulation of zinc transporters, a defence response to regulate the cellular zinc level), 2) oxidative stress (variety of defence mechanisms, triggered by Reactive Oxygen Species (ROS)), and 3) effects on the nervous system (possibly the primary lesion explaining the avoidance behaviour and also why enchytraeids are relatively susceptible to Zn). The adverse outcome at the organism level (reproduction EC50) could be predicted based on gene expression (male gonad development, oocyte maturation), with Zn at the EC50 affecting processes related to higher stress levels. The gene expression response was time-dependent and reflected the cascade of events taking place over-time. The 1 to 4 days of exposure design was a good strategy as it captured the time for sequence of events towards zinc adverse outcomes in E. crypticus.

Published
2022
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43. Risk management framework for nano-biomaterials used in medical devices and advanced therapy medicinal products

Author

Giubilato, Elisa, Cazzagon, Virginia, Amorim, Mónica J.B., Blosi, Magda, Bouillard, Jacques, Bouwmeester, Hans, Costa, Anna Luisa, Fadeel, Bengt, Fernandes, Teresa F., Fito, Carlos, Hauser, Marina, Marcomini, Antonio, Nowack, Bernd, Pizzol, Lisa, Powell, Leagh, Prina-Mello, Adriele, Sarimveis, Haralambos, Scott-Fordsmand, Janeck James, Semenzin, Elena, Stahlmecke, Burkhard, Stone, Vicki, Vignes, Alexis, Wilkins, Terry, Zabeo, Alex, Tran, Lang, Hristozov, Danail, Giubilato, Elisa, Cazzagon, Virginia, Amorim, Mónica J.B., Blosi, Magda, Bouillard, Jacques, Bouwmeester, Hans, Costa, Anna Luisa, Fadeel, Bengt, Fernandes, Teresa F., Fito, Carlos, Hauser, Marina, Marcomini, Antonio, Nowack, Bernd, Pizzol, Lisa, Powell, Leagh, Prina-Mello, Adriele, Sarimveis, Haralambos, Scott-Fordsmand, Janeck James, Semenzin, Elena, Stahlmecke, Burkhard, Stone, Vicki, Vignes, Alexis, Wilkins, Terry, Zabeo, Alex, Tran, Lang, and Hristozov, Danail

Abstract

The convergence of nanotechnology and biotechnology has led to substantial advancements in nano-biomaterials (NBMs) used in medical devices (MD) and advanced therapy medicinal products (ATMP). However, there are concerns that applications of NBMs for medical diagnostics, therapeutics and regenerative medicine could also pose health and/or environmental risks since the current understanding of their safety is incomplete. A scientific strategy is therefore needed to assess all risks emerging along the life cycles of these products. To address this need, an overarching risk management framework (RMF) for NBMs used in MD and ATMP is presented in this paper, as a result of a collaborative effort of a team of experts within the EU Project BIORIMA and with relevant inputs from external stakeholders. The framework, in line with current regulatory requirements, is designed according to state-of-the-art approaches to risk assessment and management of both nanomaterials and biomaterials. The collection/generation of data for NBMs safety assessment is based on innovative integrated approaches to testing and assessment (IATA). The framework can support stakeholders (e.g., manufacturers, regulators, consultants) in systematically assessing not only patient safety but also occupational (including healthcare workers) and environmental risks along the life cycle of MD and ATMP. The outputs of the framework enable the user to identify suitable safe(r)-by-design alternatives and/or risk management measures and to compare the risks of NBMs to their (clinical) benefits, based on efficacy, quality and cost criteria, in order to inform robust risk management decision-making.

Published
2020

48. Exploring DNA methylation patterns in copper exposed Folsomia candida and Enchytraeus crypticus

Author

One Health Toxicologie, Noordhoek, Jeroen W., Koning, Jasper T., Mariën, Janine, Kamstra, Jorke H., Amorim, Mónica J.B., van Gestel, Cornelis A.M., van Straalen, Nico M., Roelofs, Dick, One Health Toxicologie, Noordhoek, Jeroen W., Koning, Jasper T., Mariën, Janine, Kamstra, Jorke H., Amorim, Mónica J.B., van Gestel, Cornelis A.M., van Straalen, Nico M., and Roelofs, Dick

Published
2018

49. Exploring DNA methylation patterns in copper exposed Folsomia candida and Enchytraeus crypticus

Author

Noordhoek, Jeroen W., Koning, Jasper T., Mariën, Janine, Kamstra, Jorke H., Amorim, Mónica J.B., van Gestel, Cornelis A.M., van Straalen, Nico M., Roelofs, Dick, One Health Toxicologie, One Health Toxicologie, and Animal Ecology

Subjects
0301 basic medicine, Bisulfite sequencing, Soil Science, 010501 environmental sciences, Biology, 01 natural sciences, LC–MS, Folsomia candida, 03 medical and health sciences, chemistry.chemical_compound, Epigenetics, Invertebrate, Gene, Ecology, Evolution, Behavior and Systematics, 0105 earth and related environmental sciences, Genetics, DNA methylation, Enchytraeus crypticus, Methylation, Housekeeping gene, LC-MS, 030104 developmental biology, chemistry, CpG site, Cytosine, Copper
Abstract

Accumulating evidence shows that epigenetics-mediated phenotypic plasticity plays a role in an organism’s ability to deal with environmental stress. However, to date, the role of epigenetic modifications in response to stress is hardly investigated in soil invertebrates. The main objective of this proof of principle study was to explore whether total cytosine and locus-specific CpG methylation are present in two important ecotoxicological model organisms, the springtail Folsomia candida and the potworm Enchytraeus crypticus , and if so, whether methylation patterns might change with increased toxicant exposure. LC–MS/MS analyses and bisulfite sequencing were performed to identify the CpG methylation state of the organisms. We show here, for the first time, a total level of 1.4% 5-methyl cytosine methylation in the genome of E. crypticus , and an absence of both total cytosine and locus-specific CpG methylation in F. candida . In E. crypticus , methylation of CpG sites was observed in the coding sequence (CDS) of the housekeeping gene Elongation Factor 1α, while the CDS of the stress inducible Heat Shock Protein 70 gene almost lacked methylation. This confirms previous observations that DNA methylation differs between housekeeping and stress-inducible genes in invertebrates. DNA methylation patterns in E. crypticus were not affected by exposure to copper (II) sulfate pentahydrate (CuSO 4 ·5H 2 O) mixed in with LUFA 2.2 soil at sublethal effect concentrations that decreased reproduction by 10%, 20% and 50%. Although, differences in CpG methylation patterns between specific loci suggest a functional role for DNA methylation in E. crypticus , genome-wide bisulfite sequencing is needed to verify whether environmental stress affects this epigenetic hallmark.

Published
2018
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