1. Tumor necrosis factor alpha induction in human monocytes
- Author
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Oleg A. Osipovich, Natalia I. Misuno, Alexander V. Panyutich, Nikolai N. Voitenok, Gregory L. Idelson, Andrew B. Sudarikov, and Tatyana S. Kolesnikova
- Subjects
Transcription, Genetic ,Immunology ,Gene Expression ,Enzyme-Linked Immunosorbent Assay ,Cell Separation ,Biology ,Cycloheximide ,medicine.disease_cause ,Biochemistry ,chemistry.chemical_compound ,Reference Values ,Gene expression ,medicine ,Immunology and Allergy ,Bioassay ,Humans ,RNA, Messenger ,Molecular Biology ,Cells, Cultured ,Messenger RNA ,Tumor Necrosis Factor-alpha ,Hematology ,Blotting, Northern ,Molecular biology ,chemistry ,Staphylococcus aureus ,Leukocytes, Mononuclear ,Tumor necrosis factor alpha ,Biological Assay ,RNA extraction ,Percoll - Abstract
The present study was undertaken to assess the presence of tumor necrosis factor (TNF)-alpha mRNA and protein in circulating human blood monocytes and to study the TNF-alpha gene expression in human monocytes isolated by continuous Percoll gradient fractionation. The technique of RNA isolation directly from the blood samples was used to study TNF-alpha mRNA expression in circulating human blood leukocytes. It was shown that human blood leukocytes of healthy donors contained no presynthesized pool of TNF-alpha mRNA as well as no TNF-alpha protein. It was found that early pretreatment with cycloheximide interferes with TNF-alpha mRNA induction by Staphylococcus aureus.
- Published
- 1990