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Arbuscular mycorrhizal fungi (AMF) are mutualistic fungi that play important roles in plant nutrition and soil ecosystem functions. While AMF are known to benefit diverse host plants under a range of conditions, little is known about their presence in commercial almond orchards and how frequently used management practices regulate AMF root colonisation. A large-scale survey of almond orchards in the Central Valley of California was conducted to determine the extent of mycorrhizal associations with roots and the impact of orchard management practices and soil properties on AMF root colonisation rates. The roots in all orchards were colonised, with an overall average rate of 64.4%. Organically managed orchards had higher AMF root colonisation rates (73.2%) as compared with conventionally managed orchards (62.1%), primarily due to the presence of soil vegetative cover rather than organic matter inputs. Choice of rootstock and fumigation had only marginal effects while inoculation at planting increased AMF root colonisation of young trees by 27% compared to non-inoculated control. These results highlighted the ubiquitous presence of AMF in commercial almond orchards and significant interacting influences of common management practices on AMF root colonisation under field conditions. Further research into the functional implications of mycorrhizal associations in these orchards will help guide the development of management practices that increase AMF abundance and root colonisation to improve the sustainability of this rapidly expanding industry.

The inclusion of a smother crop used as a cash crop in an intercropping system may be an effective cultural control strategy for the management of weeds in organic production systems. In addition, a multilayered canopy created when intercropping species with different growth forms may limit germination cues for weed seeds and can allow for a more efficient utilization of resources that reduce competition to target crops from weeds. Watermelon (Citrullus lanatus) was evaluated for its ability to reduce weeds in a low-input organic system in Texas when planted alone or in various intercropping combinations that also included peanut (Arachis hypogaea), okra (Abelmoschus esculentus), cowpea (Vigna unguiculata), and hot pepper (Capsicum annuum). Watermelon significantly reduced total weed biomass when planted in monoculture and in all intercropping combinations compared with peanut, okra, cowpea, and pepper monocultures in year 1 of the 2-year study. Total weed biomass was reduced by 81%, 83%, 88%, and 92% in treatments containing watermelon on average as compared with pepper, peanut, okra, and cowpea grown in monoculture, respectively. Less effective weed suppression was obtained with watermelon in year 2. Pepper grown in monoculture had significantly higher weed biomass than all other treatments in year 2. Broadleaf weeds were effectively suppressed across all intercropping treatments in year 1, but nutsedges (Cyperus sp.) were consistently reduced both years, particularly when compared with monocrops with small leaf area such as pepper. The three and four species intercropping combinations consistently had high leaf area index (LAI) values, whereas pepper monoculture had significantly lower LAI values than all other treatments except for cowpea monoculture. There was a significant negative relationship between LAI and total weed biomass 33 d after last planting (r = −0.51, P < 0.01). There was a significant negative relationship between total weed biomass and total fruit yield in year 1 (r = −0.64, P < 0.01) but no significant relationship in year 2. Although findings were inconsistent in year 2 because of changes in precipitation amounts and in relative planting dates, these findings suggest that incorporating a multifunctional intercropping system that includes a low-growing vining crop such as watermelon or at least an architecturally complex mixture can optimize canopy density to reduce weed pressure from resilient perennial weeds such as nutsedge. This may offer organic producers another management tool for the control of perennial weeds.

A greenhouse experiment was conducted to evaluate the effects of water stress on leaf water potential, plant growth, and photosynthesis in purple passionflower (Passiflora incarnata). Twenty 4-L pots with two plants in each pot were arranged in a completely randomized design. Ten pots received a daily irrigation dose of 100% evapotranspiration (ET) throughout the 43-day experiment (control). The other 10 pots were subjected to a reduced irrigation (RI) treatment, which was implemented stepwise to achieve a gradual increase in stress, by irrigating them with 50% ET first, then with 25% ET and, finally, with 10% ET. The last stress phase was followed by a recovery phase in which all treatments received the same amount of water (100% ET). A lower water potential was obtained at 10% ET compared with control plants (−2.51 and −0.98 MPa, respectively). Plants in both 25% and 10% ET irrigation treatments had reduced net CO2 assimilation rates (4.25 and 3.50 μmol·m−2·s−1, respectively) than plants watered with 100% ET (8.53 and 6.77 μmol·m−2·s−1, respectively). Values of maximum carboxylation rate allowed by rubisco (ribulose-1,5-bisphosphate carboxylase/oxygenase), calculated 31 days after treatment (DAT) application (when RI plants were irrigated with 10% ET) decreased by ≈60%, whereas rate of photosynthetic electron transport and triose phosphate use (TPU) were reduced by ≈30% and ≈45% in the stress treatment compared with the control during the 10% ET irrigation period, respectively. Values of water potential and net CO2 assimilation rates in previously stressed plants were not different from the control treatment in the recovery phase, suggesting that P. incarnata plants could adapt well to landscaping situations where periods of extreme drought can be expected.

Premise While root-order approaches to fine-root classification have shown wide utility among wild plants, they have seen limited use for perennial crop plants. Moreover, inadequate characterization of fine roots across species of domesticated perennial crops has led to a knowledge gap in the understanding of evolutionary and functional patterns associated with different fine-root orders. Methods We examined fine-root traits of common horticultural fruit and nut crops: Malus ×domestica, Prunus persica, Vitus vinifera, Prunus dulcis, and Citrus ×clementina. Additional roots were sampled from 33 common perennial horticultural crops, native to tropical, subtropical, and temperate regions, to examine variation in 1st- and 2nd-order absorptive roots. Results First-order roots of grape and 1st- and 2nd-order roots of apple and peach were consistently thin, nonwoody, mycorrhizal, and had high N:C ratios. In contrast, 4th- and 5th-order roots of grape and 5th-order roots of apple and peach were woody, nonmycorrhizal, had low N:C ratios, and were thicker than lower-order roots. Among the 33 horticultural species, diameter of 1st- and 2nd-order roots varied about 15-fold, ranging from 0.04 to 0.60 mm and 0.05 to 0.89 mm respectively. This variation generally was phylogenetically conserved across plant lineages. Conclusions Collectively, our research shows that root-order characterization has considerably more utility than an arbitrary diameter cutoff for identifying roots of different functions in perennial horticultural crops. In addition, much of the variation in root diameter among species can be predicted by evolutionary relationships.

Saline irrigation water can lead to salt buildup and reduced crop yields. Halophytic plants are known to accumulate excess salts in tissues, removing them from the immediate environment. This two-phase experiment explored the feasibility of intercropping watermelon (Citrullus lanatus (Thunb.) Matsum. and Nakai var. lanatus) with halophytic species to mitigate the negative effects of saline irrigation water while providing a value-added crop. In the first experiment, six greenhouse-grown species were irrigated with water that was either deionized (0 dS m−1) or contained 3 or 6 dS m−1 of salts for 41 days and screened for growth and salt removal. Two halophytes were selected to be additively intercropped with watermelon under field conditions and irrigated with the same saline irrigation levels as the first experiment. Results indicated that garden orache (Atriplex hortensis L.) exhibited the highest growth rates and purslane (Portulaca oleracea L.) accumulated high amounts of sodium in plant tissues under saline irrigation. The field experiment showed that watermelon yields, stem water potential, and fruit quality were not affected by saline irrigation; however, the watermelon/orache intercropping treatment had significantly higher yields. These results suggest intercropping with halophytes has the potential to contribute a value-added crop without reducing watermelon yields.

A greenhouse study was conducted to evaluate the growth and development of poinsettia ‘Prestige Red’ (Euphorbia pulcherrima) grown in a commercial peat-based potting mix (Sunshine Mix #1) amended with biochar at 0%, 20%, 40%, 60%, 80%, or 100% (by volume) at four different fertigation regimes: F1: 100 to 200 mg·L−1 nitrogen (N), F2: 200 to 300 mg·L−1 N (control), F3: 300 to 400 mg·L−1 N, or F4: 400 to 500 mg·L−1 N. The experiment was a two-factor factorial design with 10 replications for each combination of biochar by fertigation. As the percentage of biochar increased, root substrate pore space and bulk density increased, while container capacity decreased. Root rot and red bract necrosis only occurred in F4 combined with 100% biochar. Plants grown in 40% biochar had a similar growth and development to those in 0% biochar. Up to 80% biochar, plants exhibited no significant change, except in terms of dry weight, which decreased at higher biochar percentages (60% and 80%). In summary, at a fertigation rate of 100 mg·L−1 N to 400 mg·L−1 N, up to 80% biochar could be used as an amendment to peat-based root substrate with acceptable growth reduction and no changes in quality.

After an outbreak of blotch leafminer (Cameraria caryaefoliella) on field-grown pecan (Carya illinoinensis) trees in 2010, an experiment was conducted to evaluate the consequences of the injury on carbon assimilation and photosynthetic efficiency, and, in particular, to assess if low-to-moderate injury induces a compensatory increase in photosynthesis. Gas exchange and light-adapted fluorescence were measured on non-injured portions of the leaflet lamina adjacent to the injured area as well as on portions of leaflets that included leafminer injury. Results indicate that damage of the photosynthetic apparatus did not extend beyond the injured areas by leafminers. Furthermore, although a strong relationship between the proportion of leafminer injury and area-based net CO2 assimilation rate of injured leaflet tissue was found, there was no evidence that pecan leaves were able to compensate for leafminer injury by upregulating CO2 assimilation in leaflet tissue that was unaffected.

Tree transplanting practices influence plant survival, establishment, and subsequent landscape value. The inability to adequately quantify effects of transplanting practices threatens long-term sustainability of landscape trees. Planting depth [i.e., location of the root collar relative to soil grade (soil surface)], is of particular concern for tree growth, development, and landscape performance. The authors of this study investigated the effects of planting depth and transplant season on landscape establishment of baldcypress [Taxodium distichum (L.) Rich.] and effects of planting depth and irrigation practices on landscape establishment of sycamore (Platanus occidentalis L.). Baldcypress planted above grade had reduced relative growth rate in height and diameter compared to those planted at or below grade during the first growing season, regardless of transplant season. Sycamore trees planted below grade had increased mortality and decreased growth compared to trees planted at grade or above grade, regardless of irrigation treatment. Even though trees of both species were grown under similar conditions, baldcypress was much more tolerant to belowgrade planting than sycamore. We suggest that this is related to the native habitat of both species, where baldcypress is frequently exposed to hypoxic conditions while sycamore is more prevalent on well-drained soils. Thus, it may be important to consider the native habitat of a species when evaluating the effect of planting depth.

Planting depth during container production may influence plant growth, establishment, and subsequent landscape value. A lack of knowledge about the effects of common transplanting practices may lead to suboptimal performance of planted landscape trees. Planting depth, i.e., location of the root collar relative to soil grade, is of particular concern for posttransplant tree growth both when transplanted to larger containers during production and after transplanting into the landscape. It is unknown whether negative effects of poor planting practices are compounded during the production phases and affect subsequent landscape establishment. This study investigated effects of planting depth during two successive phases of container production (10.8 L and 36.6 L) and eventual landscape establishment using lacebark elm (Ulmus parvifolia Jacq.). Tree growth was greater when planted at grade during the initial container (10.8 L) production phase and was reduced when planted 5 cm below grade. In the second container production phase (36.6 L), trees planted above grade had reduced growth compared with trees planted at grade or below grade. For landscape establishment, transplanting at grade to slightly below or above grade produced trees with greater height on average when compared with planting below grade or substantially above grade, whereas there was no effect on trunk diameter. Correlations between initial growth and final growth in the field suggested that substantial deviations of the original root to shoot transition from at-grade planting was more of a factor in initial establishment of lacebark elm than the up-canning practices associated with planting depth during container production.

This research shows that elevated CO2, climate warming, and management impact shoot and root nitrogen concentrations in different ways in managed pastures. Management (clipping frequency) had the strongest impact on aboveground tissue N concentrations, while the impact of the climate change drivers on shoot N concentration was interactive and varied seasonally. Green leaf N concentrations were decreased by elevated CO2 and increased by more frequent clipping. Both warming treatments increased leaf N concentrations under ambient CO2 concentrations, but did not significantly alter leaf N concentrations under elevated CO2 concentrations. Fine root N concentrations were mostly unaffected by the treatments, although elevated CO2 decreased root N concentration in deeper soil layers. The interactive nature of the climate change drivers through time, as well as the fact that root N concentration response to the treatments was entirely different from aboveground responses, highlights the complexity in predicting plant N nutrition responses to projected climate change., Forecasting the effects of climate change on nitrogen (N) cycling in pastures requires an understanding of changes in tissue N. We examined the effects of elevated atmospheric CO2 concentration, atmospheric warming and simulated grazing (clipping frequency) on aboveground and belowground tissue N concentrations and C : N ratios of a C3 pasture grass. Phalaris aquatica L. cv. ‘Holdfast’ was grown in the field in six transparent temperature gradient tunnels (18 × 1.5 × 1.5 m each), three at ambient atmospheric CO2 and three at 759 p.p.m. CO2. Within each tunnel, there were three air temperature treatments: ambient control, +2.2/+4.0 °C above ambient day/night warming and +3.0 °C continuous warming. A frequent and an infrequent clipping treatment were applied to each warming × CO2 combination. Green leaf N concentrations were decreased by elevated CO2 and increased by more frequent clipping. Both warming treatments increased leaf N concentrations under ambient CO2 concentrations, but did not significantly alter leaf N concentrations under elevated CO2 concentrations. Nitrogen resorption from leaves was decreased under elevated CO2 conditions as well as by more frequent clipping. Fine root N concentrations decreased strongly with increasing soil depth and were further decreased at the 10–60 cm soil depths by elevated CO2 concentrations. The interaction between the CO2 and warming treatments showed that leaf N concentration was affected in a non-additive manner. Changes in leaf C : N ratios were driven by changes in N concentration. Overall, the effects of CO2, warming and clipping treatments on aboveground tissue N concentrations were much greater than on belowground tissue.

Summary • This study examined the effects of warming, elevated atmospheric CO 2 and cutting regimen on the growth of Phalaris aquatica cv. Holdfast swards. • Six temperature gradient tunnels (TGT) were used to manipulate both air temperature and atmospheric CO 2 concentrations (ambient and 750 ppm). Within each tunnel, there were three temperature treatments: no warming, constant warming of +3.0 ° C and a daytime warming of 2.2 ° C combined with a night-time warming of 4.0 ° C and two defoliation frequencies. •A veraged across 20 months of growth, there was a positive effect of elevated atmospheric CO 2 (+11%), no effect of either warming treatment and a negative effect of frequent cutting ( − 19%) on total above ground biomass production. The responses to all treatments, however, were strongly seasonal. • Positive responses to CO 2 were statistically significant only in the spring, when plant growth was strongest. No evidence was found that high night-time warming had different effects on plant growth and plant responses to elevated atmospheric CO 2 , than constant warming.

Polar-desert plants experience low average air temperatures during their short growing season (4–8 °C mean July temperature). In addition, low availability of inorganic nitrogen in the soil may also limit plant growth. Our goals were to elucidate which N sources can be acquired by polar-desert plants, and how growth and N-uptake are affected by low growth temperatures. We compared rates of N-uptake and increases in mass and leaf area of two polar-desert species (Cerastium alpinum L. and Saxifraga caespitosa L.) over a period of 3 weeks when grown at two temperatures (6 °C vs. 15 °C) and supplied with either glycine, NH4 + or NO3 −. At 15 °C, plants at least doubled their leaf area, whereas there was no change in leaf area at 6 °C. Measured mean N-uptake rates varied between 0.5 nmol g−1 root DM s−1 on glycine at 15 °C and 7.5 nmol g−1 root DM s−1 on NH4 + at 15 °C. Uptake rates based upon increases in mass and tissue N concentrations showed that plants had a lower N-uptake rate at 6 °C, regardless of N source or species. We conclude that these polar-desert plants can use all three N sources to increase their leaf area and support flowering when grown at 15 °C. Based upon short-term (8 h) uptake experiments, we also conclude that the short-term capacity to take up inorganic or organic N is not reduced by low temperature (6 °C). However, net N-uptake integrated over a three-week period is severely reduced at 6 °C.

ently maintain or very quickly recover P and water uptake capacity. This behaviour is consistent with an The effects of prolonged exposure to dry surface soil overall rooting strategy where essentially no surface on the capacity of roots to take up water and phos- roots are shed following prolonged exposure to dry phorus were examined in mycorrhizal sour orange soil. (Citrus aurantium L.) seedlings grown in pots with upper and lower portions separated hydraulically. In Key words: Drought, split-root, 32P, citrus, uptake kinetics, the first experiment, upper portions of the pots were phosphorus uptake, water uptake, sour orange, Citrus either irrigated every 2‐3 d, droughted for 14 d, aurantium. droughted for 43 d, or droughted for 42 d followed by 8 d re-irrigation. Lower portions of the pots were irrig- Introduction ated and fertilized every 2‐3 d. Phosphorus uptake capacity was estimated in excised roots using 32 Pi n The soil surface layers, with their accumulation of roots, aerated 50, 750, and 1500 mM P solutions. Exposure organic matter and nutrients, commonly undergo large to dry soil had no appreciable effect on P uptake fluctuations in temperature, water content, and nutrient capacity. In the second experiment, the ability of intact availability. These fluctuations promote pulses of nutrient roots to acquire water and P in the 8 d following release by increasing microbial biomass turnover (Reid, rewatering after roots were exposed to localized 1974; Stark, 1994; Cui and Caldwell, 1997). Moisture drought for 14 and 43 d was examined. Roots were and temperature fluctuations may cause opportunities for observed non-destructively using small transparent nutrient uptake by roots in the litter, organic and surface tubes (2 cm diameter) and a rigid borescope. Soil water mineral layers to be brief and unpredictable (Grime, depletion was monitored using time-domain reflectro- 1994). There are at least two basic strategies for exploiting metry. Phosphorus (32P) was added at various depths resources in soil surface layers that periodically dry and in the soil in the upper compartment and uptake was which may develop high temperatures. One strategy is to assessed by non-destructively counting beta particle grow rapidly relatively thin inexpensive roots that have emissions from leaves using a scintillation probe. high absorptive capacity when the soil is wet, and then Similar to the first experiment, localized drought had rapidly shed these roots when the soil becomes dry or no effect on P uptake and soil water depletion in citrus otherwise unfavourable. This kind of root strategy is roots compared to continuously irrigated plants. Water commonly found in hot desert climates with desert succuand P uptake in the first few days apparently occurred lents (Huang and Nobel, 1992) and in certain desert from existing roots because of delayed production of half-shrubs like Cryptantha (personal observation). In new roots in the droughted treatment. Thus, citrus addition, many annual crops seem to exhibit this strategy ( Eissenstat and Yanai, 1997). roots exposed to extended periods of dry soil appar

Coupled surface–atmosphere models are being used with increased frequency to make predictions of tropospheric chemistry on a ‘future’ earth characterized by a warmer climate and elevated atmospheric CO 2 concentration. One of the key inputs to these models is the emission of isoprene from forest ecosystems. Most models in current use rely on a scheme by which global change is coupled to changes in terrestrial net primary productivity (NPP) which, in turn, is coupled to changes in the magnitude of isoprene emissions. In this study, we conducted measurements of isoprene emissions at three prominent global change experiments in the United States. Our results showed that growth in an atmosphere of elevated CO 2 inhibited the emission of isoprene at levels that completely compensate for possible increases in emission due to increases in aboveground NPP. Exposure to a prolonged drought caused leaves to increase their isoprene emissions despite reductions in photosynthesis, and presumably NPP. Thus, the current generation of models intended to predict the response of isoprene emission to future global change probably contain large errors. A framework is offered as a foundation for constructing new isoprene emission models based on the responses of leaf biochemistry to future climate change and elevated atmospheric CO 2 concentrations.

Changes in function as an individual root ages has important implications for understanding resource acquisition, competitive ability and optimal lifespan. Both nitrate uptake and respiration rates of differently aged fine roots of grape (Vitis rupestris x V. riparia cv. 3309 C) were measured. The resulting data were then used to simulate nitrate uptake efficiency and nutrient depletion as a function of root age. Both nitrate uptake and root respiration declined remarkably quickly with increasing root age. The decline in both N uptake and root respiration corresponded with a strong decline in root N concentration, suggesting translocation of nitrogen out of the roots. For simulations where no nutrient depletion occurs at the root surface, daily uptake efficiency was maximal at root birth and lifetime nitrate uptake efficiency slowly increased as the roots aged. Simulations of growth of roots into unoccupied soil using a solute transport model indicated the advantage of high uptake capacity in new roots under competitive conditions where nitrate availability is very transitory.

Shallow-rooted plants were studied on unirrigated modular green roof trays. Four species had 100% survival, six had varied survival rates and five had no survivors. These outcomes suggest that water conservation practices can be an effective approach for green roofs., Background and aims The application of green roof technology has become more common in the central, northwestern and eastern USA, and is now being employed across the southern USA as well. However, there is little research in the literature that evaluated plant survival on unirrigated green roofs in subtropical climates that experience frequent drought and heat stress. Here, we summarize the results of a study of plant establishment on a modular green roof in south-central Texas. Methodology Fifteen plant species were field tested in 11.4-cm-deep green roof modules on a four-storey building in College Station, Texas, with irrigation limited to the first several weeks of establishment. Climate data, plant growth and species survival were measured over three growing seasons. Principal results Four species survived growing seasons without any losses: Graptopetalum paraguayense, Malephora lutea, Manfreda maculosa and Phemeranthus calycinus. Six species experienced varying levels of mortality: Bulbine frutescens, Delosperma cooperi, Lampranthus spectabilis, Sedum kamtschaticum, Sedum mexicanum and Nassella tenuissima. Five species had no survivors: Dichondra argentea, Stemodia lanata, Myoporum parvifolium, Sedum moranense and Sedum tetractinum. Conclusions The establishment and survival of several plant species without any mortality suggests that irrigation limited to the first few weeks after planting may be an effective approach on green roofs in spite of the more challenging climatic conditions in the southern USA. Since the climate in south-central Texas had been consistently drier and warmer than normal during the study period, longer-term research on these species is recommended to expand knowledge of establishment requirements for these species under a wider range of conditions, including wetter than normal years.

An experiment was conducted to investigate the morphologic characteristics and photosynthetic response of sun and shade leaves of mature pecan [Carya illinoinensis (Wangenh.) K. Koch] trees. Treatments were established according to leaf type (sun or shade leaves) and cultivar (Pawnee and Stuart). Sun leaves were chosen from those growing on exterior portions of the tree canopy and exposed to full sunlight for most of the day [≥1500 μmol·m−2·s−1 photosynthetic photon flux (PPF)]. Shade leaves were those growing in interior parts of the tree canopy (≤100 μmol·m−2·s−1 PPF). Epidermis characteristics, leaf area, and chlorophyll (Chl) content were also measured. Results indicated that stomatal density (stomata/mm2), leaf area, and leaflet area were greater in sun leaves than in shade leaves in both cultivars investigated. Specific leaf area was greater in shade leaves than sun leaves. Chlorophyll fluorescence, total Chl content, Chl a, Chl b, and Chl a/b were unaffected by leaf type or cultivar. In both cultivars, photosynthetic light response curves showed that area-based maximum assimilation rate (Amax) in shade leaves was about half of that measured in sun leaves in June through August. However, in October, Amax of sun leaves dropped to values similar to those measured in shade leaves. Light compensation point of photosynthesis and dark respiration rate were always lower in shade leaves than in sun leaves. Overall, there were only minor differences between the cultivars. Pecan trees require careful canopy management to avoid self shading and to maintain productivity. These results could help determine optimal levels of canopy light interception and could be used to develop canopy and crop management practices.