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Flash flood early warning requires accurate rainfall forecasts with a high spatial and temporal resolution. As the first few hours ahead are already not sufficiently well captured by the rainfall forecasts of numerical weather prediction (NWP) models, rainfall nowcasting can provide an alternative. This observation-based method, however, quickly loses skill after the first few hours of the forecast due to growth and dissipation processes that are not accounted for. In addition, providing an additional forecasting method can let users drown in the amount of available information. A promising way forward is a seamless forecasting system, which combines the aforementioned forecasting methods. By optimally combining (blending) rainfall nowcasts with NWP forecasts, we can extend the skillful lead time of short-term rainfall forecasts and provide users with more consistent, seamless forecasts.We implemented an adaptive scale-dependent ensemble blending method in the open-source pysteps library. In this implementation, the blending of the extrapolation (ensemble) nowcast, (ensemble) NWP and noise components is performed level-by-level, which means that the blending weights vary per spatial cascade level. These scale-dependent blending weights are computed from the recent skill of the forecast components, and converge to a climatological value, which is computed from a multi-day rolling window and can be adjusted to the (operational) needs of the user. To constrain the (dis)appearance of rain in the ensemble members to regions around the rainy areas, we have developed a Lagrangian blended probability matching scheme and incremental masking strategy.We evaluate the method using three heavy and extreme (July 2021) rainfall events in four Belgian and Dutch catchments, focusing on both the rainfall forecasts and the resulting discharge forecasts using the fully distributed wflow_sbm hydrological model. We benchmark the results of the 48-member blended forecasts against the deterministic Belgian NWP forecast, a 48-member nowcast and a simple 48-member linear blending approach. When focusing on the resulting rainfall forecasts, the introduced blending approach predominantly performs similarly or better than only nowcasting (in terms of event-averaged CRPS and CSI values) and adds value compared to NWP for the first hours of the forecast. This holds for both the radar domain and catchment scale, although the difference, particularly with the linear blending method, reduces when we focus on catchment-average cumulative rainfall sums instead of instantaneous rainfall rates. We find similar results for the resulting discharge forecasts, although the effect of the catchment size and corresponding lag times becomes influential and determines the added value of nowcasting over NWP. By properly combining observations and NWP forecasts, blending methods such as these are a crucial component of seamless hydrometeorological forecasting systems.

Study aim: To investigate the effect of fatigue, induced by a volleyball training game on the risk of Anterior Cruciate Ligament (ACL) injury. Material and methods: Thirteen female volleyball college athletes, ages 18 to 21 years old, completed jump landings from a box 30 cm height, prior and post a 60-minute volleyball training game. The clinical tool Landing Error Scoring System (LESS) was employed in order to evaluate the technique of landing prior and post the game. The level of fatigue induced by the volleyball game was assessed by vertical jump test and Borg Rating of Perceived Exertion (RPE) Scale pre and post-game. In order to compare measurements pre and post-game t-tests for dependent samples were used. Results: Participants performed lower vertical jumps post-game with a Confidence Interval of 26.2 ± 2.3 cm (pre-game) and 24.9 ± 2.2 cm (post game). The difference between pre and post-game was found to be statistically significant with a t12 = 2.55 and a p-value of 0.026. In the case of assessing fatigue, the Borg RPE scale scores were found to be statistically significant (t12 = 14.05, p < 0.001) higher post-game (10.2 ± 0.6), as compared to pre-game (6.5 ± 0.4). Similarly, LESS scores increased significantly (t12 = 2.21, p = 0.047), post-game (6.3 ± 1.1) compared to pre-game (5.8 ± 1.0) that prove poorer landing ability. Conclusion: It seems that a short duration volleyball training game induces fatigue and negatively affects the jumping and landing ability.

This paper examines the effect of match–induced fatigue on lower limb biomechanics, in the case of a basketball game. For this purpose, sixteen male basketball athletes, ages 18 to 22, performed a jump-landing task prior and post a recreational basketball game. The Landing Error Scoring System (LESS) was used to examine the biomechanics of landing. The Vertical jump (VJ) and the Borg Rating of Perceived Exertion (RPE) scale pre- and post-game were employed to assess the level of fatigue induced by the basketball game. In order to compare pre and post measurements, t-tests for dependent samples were used. The performance of the VJ test post-game was found to be significantly lower (t (15) = 3.83, p = 0.002) showing a large effect (Cohen’s d = 0.9) compared to pre-game measurements. Further, the LESS scores were significantly (t (15) = 2.33, p = 0.034) higher post-game with a medium effect (d = 0.5). The differences in LESS scores were due to errors in the landing technique which is bound to be influenced by biomechanics. Moreover, the Borg RPE scale was found to be significantly higher (t (15) = 10.77, p < 0.001) postgame showing a very large effect (d =2.6). It is important to note, that these significant differences occurred with a merely medium level of fatigue (6.6 ± 0.3 pre-game vs 11.9 ± 1.0 post-game). The results of this study would be of great benefit to sports science teams and coaches for formulating effective strategies to improve athletes’ performance and reduce the likelihood of injury.

A single bout of exercise can result in inflammatory responses, increased oxidative stress and upregulation of enzymatic antioxidant mechanisms. Although low-volume high-intensity interval training (HIIT) has become popular, its acute responses on the above mechanisms have not been adequately studied. The present study evaluated the effects of HIIT on hematological profile and redox status compared with those following traditional continuous aerobic exercise (CET). Twelve healthy young men participated in a randomized crossover design under HIIT and CET. In HIIT session, participants performed four 30-sec sprints on a cycle-ergometer with 4 min of recovery against a resistance of 0.375 kg/kg of body mass. CET consisted of 30-min cycling on a cycle-ergometer at 70% of their VO2max. Blood was drawn at baseline, immediately post, 24h, 48h and 72h post-exercise and was analyzed for complete blood count and redox status (thiobarbituric acid reactive substances, [TBARS]; protein carbonyls, [PC]; total antioxidant capacity, [TAC]; catalase and uric acid). White blood cells (WBC) increased after both exercise protocols immediately post-exercise (HIIT: 50% and CET: 31%, respectively). HIIT increased (+22%) PC post-exercise compared to baseline and CET (p < 0.05). HIIT increased TAC immediately post-exercise (16%) and at 24h post-exercise (11%, p < 0.05), while CET increased TAC only post-exercise (12%, p < 0.05) compared to baseline, and TAC was higher following HIIT compared to CET (p < 0.05). Both HIIT and CET increased uric acid immediately post- (21% and 5%, respectively, p < 0.05) and 24h (27% and 5%, respectively, p < 0.05) post-exercise and the rise was greater following HIIT (p < 0.05). There were no significant changes (p > 0.05) for TBARS and catalase following either exercise protocol. Low-volume HIIT is associated with a greater acute phase leukocyte count and redox response than low-volume CET, and this should be considered when an exercise training program is developed and complete blood count is performed for health purposes.

The present study investigated the effect of Corinthian currant pre-exercise supplementation on metabolism, performance and blood redox status during, and after prolonged exercise. Eleven healthy participants (21-45y) performed a 90-min constant-intensity (60–70% VO2max) submaximal-trial, plus a time-trial (TT) to exhaustion (95% VO2max) after consuming an isocaloric (1.5g CHO/kg BM) amount of randomly assigned Corinthian currant or glucose-drink, or water (control). Blood was drawn at baseline, pre-exercise, 30min, 60min, 90min of submaximal-trial, post-TT, and 1h post-TT. Post-ingestion blood glucose (GLU) under Corinthian currant was higher compared with water, and similar compared with glucose-drink throughout the study. Respiratory quotient under Corinthian currant was similar with glucose-drink and higher than water throughout the submaximal trial. Accordingly, higher CHO and lower fat oxidation were observed under Corinthian currant compared with water. The TT performance was similar betwee...

Spirulina plantensis is a popular supplement which has been shown to have antioxidant and performance enhancing properties. The purpose of this study was to evaluate the effects of spirulina supplementation on (a) redox status (b) muscle performance and (c) muscle damage following an eccentric bout of exercise that would induce muscle damage. Twenty-four healthy, recreationally trained males participated in the study and were randomly separated into two groups: a spirulina supplementation (6 g per day) and a placebo group. Both groups performed an eccentric bout of exercise consisting of 5 sets and 15 maximum reps per set. Blood was collected at 24, 48, 72 and 96 h after the bout and total antioxidant capacity (TAC) and protein carbonyls (PC) were assessed in plasma. Delayed onset muscle soreness (DOMS) was also assessed at the same aforementioned time points. Eccentric peak torque (EPT) was evaluated immediately after exercise, as well as at 24, 48, 72 and 96 h post exercise. Redox status indices (TAC and PC) did not change significantly at any time point post exercise. DOMS increased significantly 24 h post exercise and remained elevated until 72 h and 96 h post exercise for the placebo and spirulina group, respectively. EPT decreased significantly and immediately post exercise and remained significantly lower compared to baseline until 72 h post exercise. No significant differences between groups were found for DOMS and EPT. These results indicate that spirulina supplementation following a muscle damaging protocol does not confer beneficial effects on redox status, muscle performance or damage.

Eccentric exercise has been shown to exert beneficial effects in both lipid profile and insulin sensitivity. Antioxidant supplementation during chronic exercise is controversial as it may prevent the physiological training-induced adaptations. The aim of this study was to investigate: 1) the minimum duration of the eccentric exercise training required before changes on metabolic parameters are observed and 2) whether antioxidant supplementation during training would interfere with these adaptations. Sixteen young healthy men were randomized into the Vit group (1 g of vitamin C and 400 IU vitamin E daily) and the placebo (PL) group. Subjects received the supplementation for 9 weeks. During weeks 5-9 all participants went through an eccentric exercise training protocol consisting of two exercise sessions (5 sets of 15 eccentric maximal voluntary contractions) per week. Plasma triglycerides (TG), total cholesterol (TC), high density lipoprotein (HDL), low density lipoprotein (LDL), apolipoproteins (Apo A1, Apo B and Lpa) and insulin sensitivity (HOMA) were assessed before the supplementation (week 0), at weeks 5, 6, 7, 8 and 9. TG, TC and LDL were significantly lower compared to pre supplementation at both weeks 8 and 9 (P

Background: For control and effective management of training process in women’s 100-m hurdles event, the coaches, in addition to detailed biomechanical parameters, need also overall, more comprehensive technical parameters, called direct performance descriptors which are used for planning the distribution of an athlete’s efforts over the race. Purpose: The aim of this study was the investigation of the race behavior of elite women sprint hurdlers, on the basis of selected time parameters, and the examination of the existence of a common race pattern in high level hurdle performance. Method: The time data of the race performance between two consecutive Olympic Games were compared. The analyzing subjects consisted of all women 100-m hurdle finalists in Athens 2004 (n = 6) and all women 100-m hurdle finalists (n = 8) and semi-finalists (n = 14) in Beijing 2008. Results: No significant differences were revealed between the two competitions concerning to the means of approach run time, run-in time, intermediate touchdown times, interval times for the hurdle units and the corresponding average velocities. Significant relationship exists between the intermediate times and final performance. The time contribution of the first half of the race to the formation of the final performance is approximately equal to the second one and, generally the standardised time parameters show the existence of a common race pattern in high level hurdle performance. Conclusion: The presented biomechanical data provide coaches and athletes with valuable information about hurdle technique for effective interventions in the training process.

Exercise-induced skeletal muscle microtrauma is characterized by loss of muscle cell integrity, marked aseptic inflammatory response, and oxidative stress. We examined if iron supplementation would alter redox status after eccentric exercise. In a randomized, double blind crossover study, that was conducted in two cycles, healthy adults (n=14) and children (n=11) received daily either 37 mg of elemental iron or placebo for 3 weeks prior to and up to 72 h after an acute eccentric exercise bout. Blood was drawn at baseline, before exercise, and 72 h after exercise for the assessment of iron status, creatine kinase activity (CK), and redox status. Iron supplementation at rest increased iron concentration and transferrin saturation (p<0.01). In adults, CK activity increased at 72 h after exercise, while no changes occurred in children. Iron supplementation increased TBARS at 72 h after exercise in both adults and children; no changes occurred under placebo condition. Eccentric exercise decreased bilirubin concentration at 72 h in all groups. Iron supplementation can alter redox responses after muscle-damaging exercise in both adults and children. This could be of great importance not only for healthy exercising individuals, but also in clinical conditions which are characterized by skeletal muscle injury and inflammation, yet iron supplementation is crucial for maintaining iron homeostasis. This study was registered at Clinicaltrials.gov Identifier:NCT02374619.

Studies of the fat mass and obesity-associated (FTO) gene provide compelling evidence of genetic variation in the general population that influences fat levels and obesity risk. Studies of the interaction between genetic and environmental factors such as physical activity (PA) will promote the understanding of how lifestyle can modulate genetic contributions to obesity. In this study, we investigated the effect of FTO genotype, and interactions with PA or energy intake, in young children and adolescents. In all, 1-5-year-old children from the Growth, Exercise and Nutrition Epidemiological Study in preSchoolers (GENESIS) study (N=1980) and 11-18-year-old Greek adolescents (N=949) were measured for adiposity-related phenotypes and genotyped at the FTO single-nucleotide polymorphism (SNP) marker, rs17817449. Adolescents were classified as physically active or inactive based on self-reported levels of PA. In adolescents, FTO genotype influenced weight (P=0.001) and BMI (P=0.007). There was also a significant SNP(*)PA(*)gender interaction (P=0.028) on BMI, which reflected the association between FTO genotype and BMI in males (P=0.016), but not females (P=0.15), and significant SNP(*)PA interaction in males (P=0.007), but not females (P=0.74). The FTO genotype effect was more pronounced in inactive than active males. Inactive males homozygous for the G allele had a mean BMI 3 kg/m(2) higher than T carriers (P=0.008). In the GENESIS study, no significant association between FTO genotype and adiposity was found. The present findings highlight PA as an important factor modifying the effect of FTO genotype.

The procedure for blood sampling in doping control, implemented for the first time as part of the official foren- sic testing programme in the Athens 2004 Summer Olympic Games is described. 743 whole blood and serum samples from 37 sports were analyzed. In serum human growth hormone and hemoglobin based oxygen carriers were screened for. In whole blood samples heterologous blood transfusion was detected. Routine haematological parameters (hematocrit, haemoglobin) were followed in a very limited range only in Modern Pentathlon under the responsibility of the respective International Federation. The majority of the blood samples collected concerned events in athletics. Endurance sports accounted for 81% of the whole blood sampling. Athletics, swimming and rowing represented 55% of the serum samples analyzed. Extension of testing for oxygen transport enhancing agents in anaerobic sports should be seriously considered by future organizers and the International Olympic Committee. The mean time for an athlete to report to the Doping Control Station for blood collection was 6.1 hours; 27% of the ath- letes tested (from 19 sports) failed to appear within the 12-hours time limit set. Training of the phlebotomists was proven effective. Only 25 athletes of those tested (3%) reported to have felt uncomfort- able. The majority of complaints (47%) concerned the prolonged waiting period and the long blood collection time (48%). Finally, an earlier agreement between all involved parties, attempts to decrease the time to appear at the site of blood col- lection and shortening of the duration of blood collection and sealing of the samples are recommended.

The functional allele (577R) of ACTN3, which encodes human alpha-actinin-3, has been reported to be associated with elite athletic status and with response to resistance training, while the nonfunctional allele (577X) has been proposed as a candidate metabolically thrifty allele. In a study of 992 adolescent Greeks, we show that there is a significant association (P=0.003) between the ACTN3 R577X polymorphism and 40 m sprint time in males that accounts for 2.3% of phenotypic variance, with the 577R allele contributing to faster times in an additive manner. The R577X polymorphism is not associated with other power phenotypes related to 40 m sprint, nor with an endurance phenotype. Furthermore, the polymorphism is not associated with obesity-related phenotypes in our population, suggesting that the 577X allele is not a thrifty allele, and thus the persistence of this null allele must be explained in other terms.

Genetic variation in the human Angiotensin I-Converting Enzyme (ACE) gene has been associated with many heritable traits, including physical performance. Herein we report the results of a study of several physical, physiological and skill parameters and lifestyle in 1027 teenage Greeks. We show that there is a strong association (P less than 0.001) between the ACE I/D (insertion/deletion) polymorphism and both handgrip strength and vertical jump in females, homozygotes for the I-allele exhibiting higher performance-related phenotype scores, accounting for up to 4.5% of the phenotypic variance. The association is best explained by a model in which the D-allele is dominant, with the mean phenotypic value in the I/D heterozygotes being close to that of the mean of the DD homozygotes. The association acts across the phenotype distribution in a classical polygenic manner. Other polymorphisms that define major ACE haplotypes in European populations (rs4424958, rs4311) show weaker associations with these performance-related phenotypes than does I/D. Similarly, diplotypes defined by these polymorphisms do not explain significantly larger amounts of the variance than I/D alone. As ACE I/D is the polymorphism most strongly associated with circulating ACE activity in European populations, we propose that the functional allelic differences that influence ACE activity also mediate the associations with the performance-related phenotypes studied here.

Genetic variation in the human angiotensin I-converting enzyme (ACE) gene has been associated with many heritable traits, including obesity. Herein, we report the results of a study of obesity-related phenotypes and lifestyle in 1016 teen-aged Greeks. We show that there is a strong association (p = 0.001) between subcutaneous fat and the ACE insertion/deletion (I/D) polymorphism in females, possession of genotypes containing the D allele being associated with increased fat thickness. This association is strongest in females who participate in no extra exercise and accounts for 6.5% of the phenotypic variance in fat thickness by ANOVA. The association is additive, with the mean phenotypic values in heterozygotes intermediate between the means of the two homozygotes, and the association acts at both extremes of the fat thickness distribution in a classical polygenic manner. Other ACE polymorphisms (rs4424958, rs4311) that define major haplotypes in European populations fail to provide stronger associations with the subcutaneous fat phenotype. Because ACE I/D is the polymorphism most strongly associated with circulating ACE levels in European populations, we propose that the functional allelic differences that influence circulating ACE levels also mediate the associations with the obesity-related phenotypes studied here.

Objective: The aim of this study was to investigate physical fitness in relation to fatness in urban and rural Greek children by means of allometric scaling. Methods: The sample consisted of 360 (189 urban and 171 rural; age 12.3±0.42 years) boys and 247 (125 urban and 122 rural; age 12.3±0.43 years) girls. The sample was highly representative (32–64%) of all 12 year old children registered in the prefecture of Trikala, Greece. All volunteers were assessed for BMI and % body fat, as well as sit and reach, basketball throw (BT), vertical jump (VJ), handgrip strength (HG), 40 m sprint, agility run, and 20 m shuttle run. To correct for possible associations between fatness and fitness, a single cause allometric scaling was employed using the natural logarithms (ln) of fitness parameters that were significantly correlated with the ln body fat. Results: Independent-samples t tests revealed that VJ (p

We investigated the effects of a single bout of aerobic and resistance exercise of similar relative intensity and duration on resting energy expenditure (REE) and substrate utilisation. Ten young healthy males volunteered [age 22 (1.8) years, weight 76 (7.9) kg, height 176 (4.1) cm, percentage body fat 10.5 (4.0)%; mean (SEM)]. They randomly underwent three conditions in which they either lifted weights for 60 min at 70-75% of 1-RM (WL), ran for 60 min at 70-75% of maximal oxygen intake (R) or did not exercise (C). REE and substrate utilisation, determined via respiratory exchange ratio ( R), were measured prior to exercise, and 10, 24, 48 and 72 h post-exercise. It was revealed that REE was significantly elevated ( P