1,468 results on '"Bozzetti, A."'
Search Results
2. Perioperative management of Takotsubo cardiomyopathy: an overview
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Marta Pillitteri, Etrusca Brogi, Chiara Piagnani, Giuseppe Bozzetti, and Francesco Forfori
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Takotsubo cardiomyopathy ,Takotsubo syndrome ,Broken heart syndrome ,Left ventricular apical ballooning syndrome ,Stress cardiomyopathy ,Perioperative period ,Anesthesiology ,RD78.3-87.3 ,Medical emergencies. Critical care. Intensive care. First aid ,RC86-88.9 - Abstract
Abstract Resembling the morphology of Japanese polyp vessels, the classic form of Takotsubo cardiomyopathy is characterized by the presence of systolic dysfunction of the mid-apical portion of the left ventricle associated with basal hyperkinesia. It is believed that this may be due to a higher density of β-adrenergic receptors in the context of the apical myocardium, which could explain the greater sensitivity of the apex to fluctuations in catecholamine levels. The syndrome is precipitated by significant emotional stress or acute severe pathologies, and it is increasingly diagnosed during the perioperative period. Indeed, surgery, induction of general anaesthesia and critical illness represent potential harmful trigger of stress cardiomyopathy. No universally accepted guidelines are currently available, and, generally, the treatment of TTS relies on health care personal experience and/or local practice. In our daily practice, anaesthesiologists can be asked to manage patients with the diagnosis of new-onset Takotsubo before elective surgery or an emergent surgery in a patient with a concomitant stress cardiomyopathy. Even more, stress cardiomyopathy can arise as a complication during the operation. In this paper, we aim to provide an overview of Takotsubo syndrome and to discuss how to manage Takotsubo during surgery and in anaesthesiologic special settings.
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- 2024
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3. Efficacia degli interventi non farmacologici anti-brivido nei pazienti sottoposti a gestione mirata della temperatura: risultati di una Scoping Review
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Dina Mahmoud Mahmoud El Lahlah, Carla Maestrini, and Mattia Bozzetti
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Terapia Intensiva ,gestione mirata della temperatura ,ipotermia da brivido ,infermieri ,infermieristica ,Nursing ,RT1-120 - Abstract
La gestione mirata della temperatura (TTM) è un intervento neuroprotettivo cruciale impiegato nelle unità di terapia intensiva per migliorare la prognosi dei pazienti dopo un arresto cardiaco. Il brivido, un effetto collaterale comune della TTM, mette in discussione i suoi benefici neuroprotettivi. Questa scoping review mira a valutare la letteratura disponibile sulle terapie non farmacologiche per il brivido nei pazienti con TTM, identificando le lacune e le potenziali aree di ricerca future. Sono stati analizzati 13 studi sul tema, che hanno coinvolto 743 soggetti, con particolare attenzione alla TTM neuroprotettiva. La revisione ha evidenziato la natura incoerente della letteratura esistente sui trattamenti non farmacologici del brivido, sottolineando la necessità di interventi efficaci supportati da studi clinici. Le coperte ad aria forzata sono emerse come un approccio frequentemente esplorato ed efficace. Lo studio fornisce una base per la ricerca futura sulle terapie non farmacologiche, sottolineando l'importanza di una valutazione oggettiva del brivido e di strumenti di misurazione validati.
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- 2024
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4. Characterization of Chemoresistance in Pancreatic Cancer: A Look at MDR-1 Polymorphisms and Expression in Cancer Cells and Patients
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Giulia Girolimetti, Barbara Balena, Paola Cordella, Tiziano Verri, Leonardo Henry Eusebi, Maria Pia Bozzetti, Cecilia Bucci, and Flora Guerra
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drug resistance ,pancreatic cancer ,transmembrane drug transporter ,ABC (MDR/TAP subfamily) transporter ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
Pancreatic malignancy is the fourth cause of cancer-related death in Western countries and is predicted to become the second leading cause of cancer-related mortality by 2030. The standard therapies (FOLFIRINOX and gemcitabine with nab-paclitaxel) are not resolutive because this type of cancer is also characterized by a high chemoresistance, due in part to the activity of the ATP Binding Cassette (ABC) pumps accounting for the reduction in the intracellular concentration of the drugs. In this work, we analyze the occurrence of single-nucleotide polymorphisms (SNPs) in the MDR-1 gene, in different pancreatic cancer cell lines, and in tissues from pancreatic cancer patients by DNA sequencing, as well as the expression levels of MDR-1 mRNA and protein, by qRT-PCR and Western Blot analysis. We found that gemcitabine-resistant cells, in conjunction with homozygosis of analyzed SNPs, showed high MDR-1 basal levels with further increases after gemcitabine treatment. Nevertheless, we did not observe in the human PDAC samples a correlation between the level of MDR-1 mRNA and protein expression and SNPs. Preliminary, we conclude that in our small cohort, these SNPs cannot be used as molecular markers for predicting the levels of MDR-1 mRNA/protein levels and drug responses in patients with PDAC.
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- 2024
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5. Technological traits and mitigation activity of autochthonous lactic acid bacteria from mediterranean fermented meat-products
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Milani, Giovanni, Tabanelli, Giulia, Barbieri, Federica, Montanari, Chiara, Gardini, Fausto, Belloso Daza, Mireya Viviana, Castellone, Vincenzo, Bozzetti, Marianna, Cocconcelli, Pier Sandro, and Bassi, Daniela
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- 2024
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6. Lesion mapping and functional characterization of hemiplegic children with different patterns of hand manipulation
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Errante, Antonino, Bozzetti, Francesca, Piras, Alessandro, Beccani, Laura, Filippi, Mariacristina, Costi, Stefania, Ferrari, Adriano, and Fogassi, Leonardo
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- 2024
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7. Lesion mapping and functional characterization of hemiplegic children with different patterns of hand manipulation
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Antonino Errante, Francesca Bozzetti, Alessandro Piras, Laura Beccani, Mariacristina Filippi, Stefania Costi, Adriano Ferrari, and Leonardo Fogassi
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Cerebral palsy ,Lesion classification ,Voxel-based morphometry ,Upper limb ,Action execution ,Functional reorganization ,Computer applications to medicine. Medical informatics ,R858-859.7 ,Neurology. Diseases of the nervous system ,RC346-429 - Abstract
Brain damage in children with unilateral cerebral palsy (UCP) affects motor function, with varying severity, making it difficult the performance of daily actions. Recently, qualitative and semi-quantitative methods have been developed for lesion classification, but studies on mild to moderate hand impairment are lacking. The present study aimed to characterize lesion topography and preserved brain areas in UCP children with specific patterns of hand manipulation. A homogeneous sample of 16 UCP children, aged 9 to 14 years, was enrolled in the study. Motor assessment included the characterization of the specific pattern of hand manipulation, by means of unimanual and bimanual measures (Kinematic Hand Classification, KHC; Manual Ability Classification System, MACS; House Functional Classification System, HFCS; Melbourne Unilateral Upper Limb Assessment, MUUL; Assisting Hand Assessment, AHA). The MRI morphological study included multiple methods: (a) qualitative lesion classification, (b) semi-quantitative classification (sq-MRI), (c) voxel-based morphometry comparing UCP and typically developed children (VBM-DARTEL), and (d) quantitative brain tissue segmentation (q-BTS). In addition, functional MRI was used to assess spared functional activations and cluster lateralization in the ipsilesional and contralesional hemispheres of UCP children during the execution of simple movements and grasping actions with the more affected hand. Lesions most frequently involved the periventricular white matter, corpus callosum, posterior limb of the internal capsule, thalamus, basal ganglia and brainstem. VMB-DARTEL analysis allowed to detect mainly white matter lesions. Both sq-MRI classification and q-BTS identified lesions of thalamus, brainstem, and basal ganglia. In particular, UCP patients with synergic hand pattern showed larger involvement of subcortical structures, as compared to those with semi-functional hand. Furthermore, sparing of gray matter in basal ganglia and thalamus was positively correlated with MUUL and AHA scores. Concerning white matter, q-BTS revealed a larger damage of fronto-striatal connections in patients with synergic hand, as compared to those with semi-functional hand. The volume of these connections was correlated to unimanual function (MUUL score). The fMRI results showed that all patients, but one, including those with cortical lesions, had activation in ipsilesional areas, regardless of lesion timing. Children with synergic hand showed more lateralized activation in the ipsilesional hemisphere both during grasping and simple movements, while children with semi-functional hand exhibited more bilateral activation during grasping. The study demonstrates that lesion localization, rather than lesion type based on the timing of their occurrence, is more associated with the functional level of hand manipulation. Overall, the preservation of subcortical structures and white matter can predict a better functional outcome. Future studies integrating different techniques (structural and functional imaging, TMS) could provide further evidence on the relation between brain reorganization and specific pattern of manipulation in UCP children.
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- 2024
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8. TBC1D32 variants disrupt retinal ciliogenesis and cause retinitis pigmentosa
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Béatrice Bocquet, Caroline Borday, Nejla Erkilic, Daria Mamaeva, Alicia Donval, Christel Masson, Karine Parain, Karolina Kaminska, Mathieu Quinodoz, Irene Perea-Romero, Gema Garcia-Garcia, Carla Jimenez-Medina, Hassan Boukhaddaoui, Arthur Coget, Nicolas Leboucq, Giacomo Calzetti, Stefano Gandolfi, Antonio Percesepe, Valeria Barili, Vera Uliana, Marco Delsante, Francesca Bozzetti, Hendrik P.N. Scholl, Marta Corton, Carmen Ayuso, Jose M. Millan, Carlo Rivolta, Isabelle Meunier, Muriel Perron, and Vasiliki Kalatzis
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Genetics ,Ophthalmology ,Medicine - Abstract
Retinitis pigmentosa (RP) is the most common inherited retinal disease (IRD) and is characterized by photoreceptor degeneration and progressive vision loss. We report 4 patients presenting with RP from 3 unrelated families with variants in TBC1D32, which to date has never been associated with an IRD. To validate TBC1D32 as a putative RP causative gene, we combined Xenopus in vivo approaches and human induced pluripotent stem cell–derived (iPSC-derived) retinal models. Our data showed that TBC1D32 was expressed during retinal development and that it played an important role in retinal pigment epithelium (RPE) differentiation. Furthermore, we identified a role for TBC1D32 in ciliogenesis of the RPE. We demonstrated elongated ciliary defects that resulted in disrupted apical tight junctions, loss of functionality (delayed retinoid cycling and altered secretion balance), and the onset of an epithelial-mesenchymal transition–like phenotype. Last, our results suggested photoreceptor differentiation defects, including connecting cilium anomalies, that resulted in impaired trafficking to the outer segment in cones and rods in TBC1D32 iPSC-derived retinal organoids. Overall, our data highlight a critical role for TBC1D32 in the retina and demonstrate that TBC1D32 mutations lead to RP. We thus identify TBC1D32 as an IRD-causative gene.
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- 2023
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9. Undergraduate nurses attitudes towards organ donation: a cross-sectional survey in a north Italian university.
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Mattia Bozzetti, Martina Caraffini, Chiara Agosti, Ilaria Marcomini, and Roberta Pendoni
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Organ donation ,donors ,transplant ,attitudes ,nursing students ,Nursing ,RT1-120 - Abstract
BACKGROUND: Although research reveals that there are many complex factors influencing the low organ donation rate in addition to nurses, literature suggests that nurses and doctors' active engagement and support have a significant impact on donation rates. Donation rates can be influenced by a variety of factors, including health professionals' attitudes, knowledge, confidence, participation, and training. OBJECTIVES: To investigate the attitudes of undergraduate nursing students at the University of Brescia in the Campus of Cremona regarding organ and tissue donation. MATERIALS AND METHODS: A cross-sectional survey was conducted by administering the Organ Donation Attitudes Scale (ODAS). RESULTS: ODAS showed that most students know someone who has donated an organ during their lifetime or after death, and over half know someone who has received an organ transplant. However, 63.3% of students indicated that they had not received specific training, and attitudes ranged from 45 to 71 with a median score of 61. Bivariate correlation analysis revealed that received academic training does not correlate with students' attitudes (p=.37). Knowing someone who has received a transplant correlates with having expressed willingness to donate (p=.047), and academic training correlates with perceived knowledge about the donation process (p=.0001). CONCLUSIONS: The study found mainly positive attitudes toward organ and tissue donation among nursing undergraduate students from the Cremona campus. Further research involving nursing universities is needed to raise awareness of organ donation among the public and healthcare workers.
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- 2023
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10. La qualità dell’assistenza infermieristica in ambito oncologico: protocollo di validazione dello strumento Quality of Oncology Nursing Care Scale (QONCS)
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Ilaria Marcomini, Francesca Riboni, Maria Mallio, Mattia Bozzetti, Roberta Pendoni, and Laura Milani
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qualità dell'assistenza infermieristica ,oncologia ,QONCS ,Strumenti di valutazione ,validazione ,Nursing ,RT1-120 - Abstract
BACKGROUND: Gli infermieri hanno un ruolo fondamentale nella cura delle persone affette da patologia oncologica. Fornire un’assistenza infermieristica di qualità significa provvedere a rispondere in modo pertinente ai bisogni fisici e psicosociali dell’assistito. Lo strumento Quality of Oncology Nursing Care Scale (QONCS) è il primo strumento ad essere costruito su un modello concettuale consolidato che include i principali temi emersi da studi precedenti aventi per oggetto la qualità dell’assistenza infermieristica in ambito oncologico. Non esiste ad oggi in Italia uno strumento capace di misurare la qualità dell’assistenza infermieristica percepita dalla persona affetta da patologia oncologica. OBIETTIVI: Finalità generale dello studio è adattare nel contesto italiano lo strumento QONCS al fine di indagare la qualità dell’assistenza infermieristica percepita dagli assistiti oncologici e i fattori che influiscono su di essa. METODI: Lo studio è articolato in quattro fasi: (a) l’adattamento culturale dello strumento al contesto italiano; (b) la valutazione della validità di facciata e contenuto dello strumento; (c) la valutazione della validità di costrutto e affidabilità dello strumento e (d) la descrizione della qualità dell’assistenza infermieristica come percepita dagli assistiti includendo fattori/item confermati nel processo di validazione. ESITI ATTESI: Si intende validare lo strumento su più di 400 persone affette da patologia oncologica. Il costrutto che lo strumento intende misurare sarà confermato attraverso analisi fattoriale esplorativa e confermativa e riporterà una elevata consistenza interna e affidabilità test-retest.
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- 2023
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11. Molecular Signature of Monocytes Shaped by the Shigella sonnei 1790-Generalized Modules for Membrane Antigens Vaccine
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Serena Tondi, Emilio Siena, Ahmed Essaghir, Benoît Bozzetti, Viviane Bechtold, Aline Scaillet, Bruna Clemente, Mariateresa Marrocco, Chiara Sammicheli, Simona Tavarini, Francesca Micoli, Davide Oldrini, Alfredo Pezzicoli, Martina Di Fede, Michela Brazzoli, Cristina Ulivieri, and Francesca Schiavetti
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Generalized Modules for Membrane Antigens (GMMA) ,Shigella sonnei ,vaccine ,immunity ,monocytes ,transcriptomics ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
Shigellosis, an acute gastroenteritis infection caused by Shigella species, remains a public health burden in developing countries. Recently, many outbreaks due to Shigella sonnei multidrug-resistant strains have been reported in high-income countries, and the lack of an effective vaccine represents a major hurdle to counteract this bacterial pathogen. Vaccine candidates against Shigella sonnei are under clinical development, including a Generalized Modules for Membrane Antigens (GMMA)-based vaccine. The mechanisms by which GMMA-based vaccines interact and activate human immune cells remain elusive. Our previous study provided the first evidence that both adaptive and innate immune cells are targeted and functionally shaped by the GMMA-based vaccine. Here, flow cytometry and confocal microscopy analysis allowed us to identify monocytes as the main target population interacting with the S. sonnei 1790-GMMA vaccine on human peripheral blood. In addition, transcriptomic analysis of this cell population revealed a molecular signature induced by 1790-GMMA mostly correlated with the inflammatory response and cytokine-induced processes. This also impacts the expression of genes associated with macrophages’ differentiation and T cell regulation, suggesting a dual function for this vaccine platform both as an antigen carrier and as a regulator of immune cell activation and differentiation.
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- 2024
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12. Notch-Jagged1 signaling and response to bevacizumab therapy in advanced colorectal cancer: A glance to radiomics or back to physiopathology?
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Francesca Negri, Lorena Bottarelli, Giuseppe Pedrazzi, Michele Maddalo, Ludovica Leo, Gianluca Milanese, Roberto Sala, Michele Lecchini, Nicoletta Campanini, Cecilia Bozzetti, Andrea Zavani, Gianluca Di Rienzo, Cinzia Azzoni, Enrico Maria Silini, Nicola Sverzellati, Federica Gaiani, Gian Luigi de’ Angelis, and Letizia Gnetti
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Notch signaling pathway ,bevacizumab ,colorectal cancer ,Jagged-1 ,therapy resistance ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
IntroductionThe Notch intracellular domain (NICD) and its ligands Jagged-1(Jag1), Delta-like ligand (DLL-3) and DLL4 play an important role in neoangiogenesis. Previous studies suggest a correlation between the tissue levels of NICD and response to therapy with bevacizumab in colorectal cancer (CRC). Another marker that may predict outcome in CRC is radiomics of liver metastases. The aim of this study was to investigate the expression of NICD and its ligands and the role of radiomics in the selection of treatment-naive metastatic CRC patients receiving bevacizumab.MethodsImmunohistochemistry (IHC) for NICD, Jag1 and E-cadherin was performed on the tissue microarrays (TMAs) of 111 patients with metastatic CRC treated with bevacizumab and chemotherapy. Both the intensity and the percentage of stained cells were evaluated. The absolute number of CD4+ and CD8+ lymphocytes was counted in three different high-power fields and the mean values obtained were used to determine the CD4/CD8 ratio. The positivity of tumor cells to DLL3 and DLL4 was studied. The microvascular density (MVD) was assessed in fifteen cases by counting the microvessels at 20x magnification and expressed as MVD score. Abdominal CT scans were retrieved and imported into a dedicated workstation for radiomic analysis. Manually drawn regions of interest (ROI) allowed the extraction of radiomic features (RFs) from the tumor.ResultsA positive association was found between NICD and Jag1 expression (p < 0.001). Median PFS was significantly shorter in patients whose tumors expressed high NICD and Jag1 (6.43 months vs 11.53 months for negative cases; p = 0.001). Those with an MVD score ≥5 (CD31-high, NICD/Jag1 positive) experienced significantly poorer survival. The radiomic model developed to predict short and long-term survival and PFS yielded a ROC-AUC of 0.709; when integrated with clinical and histopathological data, the integrated model improved the predictive score (ROC-AUC of 0.823).DiscussionThese results show that high NICD and Jag1 expression are associated with progressive disease and early disease progression to anti VEGF-based therapy; the preliminary radiomic analyses show that the integration of quantitative information with clinical and histological data display the highest performance in predicting the outcome of CRC patients.
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- 2023
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13. Effectiveness of action observation therapy based on virtual reality technology in the motor rehabilitation of paretic stroke patients: a randomized clinical trial
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Errante, Antonino, Saviola, Donatella, Cantoni, Matteo, Iannuzzelli, Katia, Ziccarelli, Settimio, Togni, Fabrizio, Simonini, Marcello, Malchiodi, Carolina, Bertoni, Debora, Inzaghi, Maria Grazia, Bozzetti, Francesca, Menozzi, Roberto, Quarenghi, Annamaria, Quarenghi, Paola, Bosone, Daniele, Fogassi, Leonardo, Salvi, Gian Piero, and De Tanti, Antonio
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- 2022
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14. Effectiveness of action observation therapy based on virtual reality technology in the motor rehabilitation of paretic stroke patients: a randomized clinical trial
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Antonino Errante, Donatella Saviola, Matteo Cantoni, Katia Iannuzzelli, Settimio Ziccarelli, Fabrizio Togni, Marcello Simonini, Carolina Malchiodi, Debora Bertoni, Maria Grazia Inzaghi, Francesca Bozzetti, Roberto Menozzi, Annamaria Quarenghi, Paola Quarenghi, Daniele Bosone, Leonardo Fogassi, Gian Piero Salvi, and Antonio De Tanti
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Action observation therapy ,Virtual reality ,Mirror neuron system ,Stroke ,Motor learning ,Neurology. Diseases of the nervous system ,RC346-429 - Abstract
Abstract Background The rehabilitation of paretic stroke patients uses a wide range of intervention programs to improve the function of impaired upper limb. A new rehabilitative approach, called action observation therapy (AOT) is based on the discovery of mirror neurons and has been used to improve the motor functions of adult stroke patients and children with cerebral palsy. Recently, virtual reality (VR) has provided the potential to increase the frequency and effectiveness of rehabilitation treatment by offering challenging and motivating tasks. Methods The purpose of the present project is to design a randomized controlled six-month follow-up trial (RCT) to evaluate whether action observation (AO) added to standard VR (AO + VR) is effective in improving upper limb function in patients with stroke, compared with a control treatment consisting of observation of naturalistic scenes (CO) without any action content, followed by VR training (CO + VR). Discussion AO + VR treatment may provide an addition to the rehabilitative interventions currently available for recovery after stroke and could be utilized within standard sensorimotor training or in individualized tele-rehabilitation. Trial registration The trial has been prospectively registered on ClinicalTrials.gov. NCT05163210 . 17 December 2021.
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- 2022
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15. Seasonal observation and source apportionment of carbonaceous aerosol from forested rural site (Lithuania)
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Masalaite, A., Byčenkienė, S., Pauraitė, J., Garbariene, I., Haddad, I. el, Bozzetti, C., Jaffrezo, J.L., Besombes, J.L., Plauškaitė-Šukienė, K., Garbaras, A., Šapolaitė, J., Ežerinskis, Ž., Dudoitis, V., Bariseviciute, R., Ulevičius, V., Prevot, A.S.H., and Remeikis, V.
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- 2022
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16. Coverings for $4$-dimensional almost complex manifolds with non-degenerate torsion
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Bozzetti, Cristina and Medori, Costantino
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Mathematics - Differential Geometry ,Mathematics - Complex Variables - Abstract
An almost complex manifolds $(M^4,J)$ of real dimension 4 with non-degenerate torsion bundle admit a double absolute parallelism and it is provided the classification of homogeneous $(M^4,J)$ having an associated non-solvable Lie algebra. We extend such a classification to the analysis of the manifolds having an associated solvable Lie algebra, up-to-coverings. Moreover, for homogeneous $(M^4,J)$ we provide examples with connected and non-connected double covering, thus proving that in general the double absolute parallelism is not the restriction of two absolute parallelisms. Furthermore, it is given the definition of a natural metric induced by the absolute parallelisms on $(M^4,J)$ and an example of an almost complex manifold with non-degenerate torsion endowed with that metric such that it becomes an almost K\"ahler manifold., Comment: 17 pp
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- 2017
17. Characterization of Chemoresistance in Pancreatic Cancer: A Look at MDR-1 Polymorphisms and Expression in Cancer Cells and Patients.
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Girolimetti, Giulia, Balena, Barbara, Cordella, Paola, Verri, Tiziano, Eusebi, Leonardo Henry, Bozzetti, Maria Pia, Bucci, Cecilia, and Guerra, Flora
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DRUG resistance in cancer cells ,GENE expression ,WESTERN immunoblotting ,SINGLE nucleotide polymorphisms ,PANCREATIC cancer - Abstract
Pancreatic malignancy is the fourth cause of cancer-related death in Western countries and is predicted to become the second leading cause of cancer-related mortality by 2030. The standard therapies (FOLFIRINOX and gemcitabine with nab-paclitaxel) are not resolutive because this type of cancer is also characterized by a high chemoresistance, due in part to the activity of the ATP Binding Cassette (ABC) pumps accounting for the reduction in the intracellular concentration of the drugs. In this work, we analyze the occurrence of single-nucleotide polymorphisms (SNPs) in the MDR-1 gene, in different pancreatic cancer cell lines, and in tissues from pancreatic cancer patients by DNA sequencing, as well as the expression levels of MDR-1 mRNA and protein, by qRT-PCR and Western Blot analysis. We found that gemcitabine-resistant cells, in conjunction with homozygosis of analyzed SNPs, showed high MDR-1 basal levels with further increases after gemcitabine treatment. Nevertheless, we did not observe in the human PDAC samples a correlation between the level of MDR-1 mRNA and protein expression and SNPs. Preliminary, we conclude that in our small cohort, these SNPs cannot be used as molecular markers for predicting the levels of MDR-1 mRNA/protein levels and drug responses in patients with PDAC. [ABSTRACT FROM AUTHOR]
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- 2024
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18. Time-dependent source apportionment of submicron organic aerosol for a rural site in an alpine valley using a rolling positive matrix factorisation (PMF) window
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G. Chen, Y. Sosedova, F. Canonaco, R. Fröhlich, A. Tobler, A. Vlachou, K. R. Daellenbach, C. Bozzetti, C. Hueglin, P. Graf, U. Baltensperger, J. G. Slowik, I. El Haddad, and A. S. H. Prévôt
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Physics ,QC1-999 ,Chemistry ,QD1-999 - Abstract
We collected 1 year of aerosol chemical speciation monitor (ACSM) data in Magadino, a village located in the south of the Swiss Alpine region, one of Switzerland's most polluted areas. We analysed the mass spectra of organic aerosol (OA) by positive matrix factorisation (PMF) using Source Finder Professional (SoFi Pro) to retrieve the origins of OA. Therein, we deployed a rolling algorithm, which is closer to the measurement, to account for the temporal changes in the source profiles. As the first-ever application of rolling PMF with multilinear engine (ME-2) analysis on a yearlong dataset that was collected from a rural site, we resolved two primary OA factors (traffic-related hydrocarbon-like OA (HOA) and biomass burning OA (BBOA)), one mass-to-charge ratio (m/z) 58-related OA (58-OA) factor, a less oxidised oxygenated OA (LO-OOA) factor, and a more oxidised oxygenated OA (MO-OOA) factor. HOA showed stable contributions to the total OA through the whole year ranging from 8.1 % to 10.1 %, while the contribution of BBOA showed an apparent seasonal variation with a range of 8.3 %–27.4 % (highest during winter, lowest during summer) and a yearly average of 17.1 %. OOA (sum of LO-OOA and MO-OOA) contributed 71.6 % of the OA mass, varying from 62.5 % (in winter) to 78 % (in spring and summer). The 58-OA factor mainly contained nitrogen-related variables which appeared to be pronounced only after the filament switched. However, since the contribution of this factor was insignificant (2.1 %), we did not attempt to interpolate its potential source in this work. The uncertainties (σ) for the modelled OA factors (i.e. rotational uncertainty and statistical variability in the sources) varied from ±4 % (58-OA) to a maximum of ±40 % (LO-OOA). Considering that BBOA and LO-OOA (showing influences of biomass burning in winter) had significant contributions to the total OA mass, we suggest reducing and controlling biomass-burning-related residential heating as a mitigation strategy for better air quality and lower PM levels in this region or similar locations. In Appendix A, we conduct a head-to-head comparison between the conventional seasonal PMF analysis and the rolling mechanism. We find similar or slightly improved results in terms of mass concentrations, correlations with external tracers, and factor profiles of the constrained POA factors. The rolling results show smaller scaled residuals and enhanced correlations between OOA factors and corresponding inorganic salts compared to those of the seasonal solutions, which was most likely because the rolling PMF analysis can capture the temporal variations in the oxidation processes for OOA components. Specifically, the time-dependent factor profiles of MO-OOA and LO-OOA can well explain the temporal viabilities of two main ions for OOA factors, m/z 44 (CO2+) and m/z 43 (mostly C2H3O+). Therefore, this rolling PMF analysis provides a more realistic source apportionment (SA) solution with time-dependent OA sources. The rolling results also show good agreement with offline Aerodyne aerosol mass spectrometer (AMS) SA results from filter samples, except for in winter. The latter discrepancy is likely because the online measurement can capture the fast oxidation processes of biomass burning sources, in contrast to the 24 h filter samples. This study demonstrates the strengths of the rolling mechanism, provides a comprehensive criterion list for ACSM users to obtain reproducible SA results, and is a role model for similar analyses of such worldwide available data.
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- 2021
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19. Diagnostic criteria for cancer cachexia: reduced food intake and inflammation predict weight loss and survival in an international, multi‐cohort analysis
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Lisa Martin, Maurizio Muscaritoli, Isabelle Bourdel‐Marchasson, Catherine Kubrak, Barry Laird, Bruno Gagnon, Martin Chasen, Ioannis Gioulbasanis, Ola Wallengren, Anne C. Voss, Francois Goldwasser, R. Thomas Jagoe, Chris Deans, Federico Bozzetti, Florian Strasser, Lene Thoresen, Sean Kazemi, Vickie Baracos, and Pierre Senesse
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Cancer ,Cachexia ,Malnutrition ,Reduced food intake ,Inflammation ,Weight loss ,Diseases of the musculoskeletal system ,RC925-935 ,Human anatomy ,QM1-695 - Abstract
Abstract Background Cancer‐associated weight loss (WL) associates with increased mortality. International consensus suggests that WL is driven by a variable combination of reduced food intake and/or altered metabolism, the latter often represented by the inflammatory biomarker C‐reactive protein (CRP). We aggregated data from Canadian and European research studies to evaluate the associations of reduced food intake and CRP with cancer‐associated WL (primary endpoint) and overall survival (OS, secondary endpoint). Methods The data set included a total of 12,253 patients at risk for cancer‐associated WL. Patient‐reported WL history (% in 6 months) and food intake (normal, moderately, or severely reduced) were measured in all patients; CRP (mg/L) and OS were measured in N = 4960 and N = 9952 patients, respectively. All measures were from a baseline assessment. Clinical variables potentially associated with WL and overall survival (OS) including age, sex, cancer diagnosis, disease stage, and performance status were evaluated using multinomial logistic regression MLR and Cox proportional hazards models, respectively. Results Patients had a mean weight change of −7.3% (±7.1), which was categorized as: ±2.4% (stable weight; 30.4%), 2.5–5.9% (19.7%), 6.0–10.0% (23.2%), 11.0–14.9% (12.0%), ≥15.0% (14.6%). Normal food intake, moderately, and severely reduced food intake occurred in 37.9%, 42.8%, and 19.4%, respectively. In MLR, severe WL (≥15%) (vs. stable weight) was more likely (P 100 mg/L: OR 2.30 (95% CI 1.62–3.26)]. Diagnosis, stage, and performance status, but not age or sex, were significantly associated with WL. Median OS was 9.9 months (95% CI 9.5–10.3), with median follow‐up of 39.7 months (95% CI 38.8–40.6). Moderately and severely reduced food intake and CRP independently predicted OS (P
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- 2021
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20. Chronic fatigue syndrome: an emerging sequela in COVID-19 survivors?
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Mantovani, Elisa, Mariotto, Sara, Gabbiani, Daniele, Dorelli, Gianluigi, Bozzetti, Silvia, Federico, Angela, Zanzoni, Serena, Girelli, Domenico, Crisafulli, Ernesto, Ferrari, Sergio, and Tamburin, Stefano
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- 2021
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21. Supplemental parenteral nutrition in cancer care: why, who, when
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Paolo Cotogni, Federico Bozzetti, François Goldwasser, Paula Jimenez-Fonseca, Sine Roelsgaard Obling, and Juan W. Valle
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Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
Malnutrition is an often-overlooked challenge for patients with cancer. It is associated with muscle mass reduction, poor compliance and response to cancer treatments, decreased quality of life, and reduced survival time. The nutritional assessment and intervention should be a vital part of any comprehensive cancer treatment plan. However, data on artificial nutrition supplied based on caloric needs during cancer care are scarce. In this review, we discuss the recommendations of the European and American societies for clinical nutrition on the use of nutritional interventions in malnourished patients with cancer in the context of current clinical practice. In particular, when enteral nutrition (oral or tube feeding) is not feasible or fails to meet the complete nutritional needs, supplemental parenteral nutrition (SPN) can bridge the gap. We report the available evidence on SPN in cancer patients and identify the perceived barriers to the wider application of this intervention. Finally, we suggest a ‘permissive’ role of SPN in cancer care but highlight the need for rigorous clinical studies to further evaluate the use of SPN in different populations of cancer patients.
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- 2022
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22. Quality of life in young and middle age adult temporomandibular disorders patients and asymptomatic subjects: a systematic review and meta-analysis
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Lucas Bozzetti Pigozzi, Duziene Denardini Pereira, Marcos Pascoal Pattussi, Carmen Moret-Tatay, Tatiana Quarti Irigaray, João Batista Blessmann Weber, Patrícia Krieger Grossi, and Márcio Lima Grossi
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Systematic review ,Meta-analysis ,Quality of life ,Temporomandibular joint disorders ,Epidemiology ,Computer applications to medicine. Medical informatics ,R858-859.7 - Abstract
Abstract Aims To compare the difference in the quality of life between temporomandibular disorders (TMD) patients and non-TMD subjects diagnosed with the Research Diagnostic Criteria for Temporomandibular Disorders (RDC/TMD) or the Diagnostic Criteria for Temporomandibular Disorders (DC/TMD). Methods Medical Literature Analysis and Retrieval System Online (MEDLINE), Excerpta Medica database (EMBASE) and Latin American and Caribbean Health Sciences Literature (LILACS) databases were searched in studies published in English and Portuguese. The search was performed by two independent reviewers in duplicate. A manual search and the gray literature were also included. The inclusion criteria were clinical studies that used the RDC/TMD axis I and quality of life with standard questionnaires in young and middle-aged adult population (18–55 years). The data were analyzed quantitatively by combining the results in a meta-analysis using forest plots. The measure of effect used was the standardized mean difference (SMD) in depression levels. The Newcastle–Ottawa Scale (NOS) was used to evaluate the quality of the studies. The publication bias was assessed by funnel plots. The initial search included 806 articles without duplications. Results Twenty-four articles were included in the final systematic review. Of these, 9 were included in the meta-analysis, where it was shown a statistically significant in all axis I groups: (a) global TMD—groups I, II and III combined, N = 3829, SMD (95% CI) = 1.06 (0.65–1.51), p = 0.000; (b) group I—muscle disorders, N = 3,056, SMD (95% CI) = 0.82 (0.45–1.18), p = 0.000; (c) group II—disc displacements, N = 3,184, SMD (95% CI) = 0.59 (0.26–0.91), p = 0.000; and (d) group III—arthralgia/arthritis/arthrosis, N = 2781, SMD (95% CI) = 0.98 (0.59–1.36), p = 0.000. When compared to controls. Conclusions Quality of life is affected in all axis I TMD patients, especially in groups I and III with higher pain intensity and disability as compared to group II.
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- 2021
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23. A new method for long-term source apportionment with time-dependent factor profiles and uncertainty assessment using SoFi Pro: application to 1 year of organic aerosol data
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F. Canonaco, A. Tobler, G. Chen, Y. Sosedova, J. G. Slowik, C. Bozzetti, K. R. Daellenbach, I. El Haddad, M. Crippa, R.-J. Huang, M. Furger, U. Baltensperger, and A. S. H. Prévôt
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Environmental engineering ,TA170-171 ,Earthwork. Foundations ,TA715-787 - Abstract
A new methodology for performing long-term source apportionment (SA) using positive matrix factorization (PMF) is presented. The method is implemented within the SoFi Pro software package and uses the multilinear engine (ME-2) as a PMF solver. The technique is applied to a 1-year aerosol chemical speciation monitor (ACSM) dataset from downtown Zurich, Switzerland. The measured organic aerosol mass spectra were analyzed by PMF using a small (14 d) and rolling PMF window to account for the temporal evolution of the sources. The rotational ambiguity is explored and the uncertainties of the PMF solutions were estimated. Factor–tracer correlations for averaged seasonal results from the rolling window analysis are higher than those retrieved from conventional PMF analyses of individual seasons, highlighting the improved performance of the rolling window algorithm for long-term data. In this study four to five factors were tested for every PMF window. Factor profiles for primary organic aerosol from traffic (HOA), cooking (COA) and biomass burning (BBOA) were constrained. Secondary organic aerosol was represented by either the combination of semi-volatile and low-volatility organic aerosol (SV-OOA and LV-OOA, respectively) or by a single OOA when this separation was not robust. This scheme led to roughly 40 000 PMF runs. Full visual inspection of all these PMF runs is unrealistic and is replaced by predefined user-selected criteria, which allow factor sorting and PMF run acceptance/rejection. The selected criteria for traffic (HOA) and BBOA were the correlation with equivalent black carbon from traffic (eBCtr) and the explained variation of m/z 60, respectively. COA was assessed by the prominence of a lunchtime concentration peak within the diurnal cycle. SV-OOA and LV-OOA were evaluated based on the fractions of m/z 43 and 44 in their respective factor profiles. Seasonal pre-tests revealed a non-continuous separation of OOA into SV-OOA and LV-OOA, in particular during the warm seasons. Therefore, a differentiation between four-factor solutions (HOA, COA, BBOA and OOA) and five-factor solutions (HOA, COA, BBOA, SV-OOA and LV-OOA) was also conducted based on the criterion for SV-OOA. HOA and COA contribute between 0.4–0.7 µg m−3 (7.8 %–9.0 %) and 0.7–1.2 µg m−3 (12.2 %–15.7 %) on average throughout the year, respectively. BBOA shows a strong yearly cycle with the lowest mean concentrations in summer (0.6 µg m−3, 12.0 %), slightly higher mean concentrations during spring and fall (1.0 and 1.5 µg m−3, or 15.6 % and 18.6 %, respectively), and the highest mean concentrations during winter (1.9 µg m−3, 25.0 %). In summer, OOA is separated into SV-OOA and LV-OOA, with mean concentrations of 1.4 µg m−3 (26.5 %) and 2.2 µg m−3 (40.3 %), respectively. For the remaining seasons the seasonal concentrations of SV-OOA, LV-OOA and OOA range from 0.3 to 1.1 µg m−3 (3.4 %–15.9 %), from 0.6 to 2.2 µg m−3 (7.7 %–33.7 %) and from 0.9 to 3.1 µg m−3 (13.7 %–39.9 %), respectively. The relative PMF errors modeled for this study for HOA, COA, BBOA, LV-OOA, SV-OOA and OOA are on average ±34 %, ±27 %, ±30 %, ±11 %, ±25 % and ±12 %, respectively.
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- 2021
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24. Efficacy of non-pharmacological antishivering interventions in patients undergoing targeted temperature management: findings from a Scoping Review.
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El Lahlah, Dina Mahmoud Mahmoud, Maestrini, Carla, and Bozzetti, Mattia
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- 2024
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25. Non-traditional Students Between Online and Offline: Which Way Forward for Higher Education?
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Bozzetti, Alessandro, De Luigi, Nicola, and Vergolini, Loris
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NONFORMAL education ,NONTRADITIONAL college students ,TEACHING methods ,COVID-19 pandemic ,HIGHER education ,ONLINE education - Abstract
The expansion of higher education has led to a more diverse student population, theorized around the concept of the non-traditional student. This term is used to describe students whose socio-demographic characteristics, motivations, study engagement and experiences differ from those of traditional higher education participants. The non-traditional student population is a highly heterogeneous group in which the individual student presents with specific motivations, needs, and constraints, but a common requirement is for more flexible teaching and learning methods to meet their complex educational needs. We here examine this demand for flexibility through the preferences students express for online teaching methods, and we investigate whether differences between traditional and non-traditional students are mainly due to inequalities, the role of parental education in particular, or on the contrary, whether they are related to certain characteristics such as age, employment and residential status. The data used in this investigation was collected during the period characterized by the containment measures linked to the Covid-19 pandemic from students enrolled at the University of Bologna. The results of the investigation presented below confirm that non-traditional students exhibit a clear preference for online as opposed to face-to-face learning and that parental education is particularly relevant for those under 25. [ABSTRACT FROM AUTHOR]
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- 2024
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26. Does nutrition for cancer patients feed the tumour? A clinical perspective
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Bozzetti, F. and Stanga, Z.
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- 2020
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27. Molecular Signature of Monocytes Shaped by the Shigella sonnei 1790-Generalized Modules for Membrane Antigens Vaccine
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Tondi, Serena, primary, Siena, Emilio, additional, Essaghir, Ahmed, additional, Bozzetti, Benoît, additional, Bechtold, Viviane, additional, Scaillet, Aline, additional, Clemente, Bruna, additional, Marrocco, Mariateresa, additional, Sammicheli, Chiara, additional, Tavarini, Simona, additional, Micoli, Francesca, additional, Oldrini, Davide, additional, Pezzicoli, Alfredo, additional, Di Fede, Martina, additional, Brazzoli, Michela, additional, Ulivieri, Cristina, additional, and Schiavetti, Francesca, additional
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- 2024
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28. Quality of life in young and middle age adult temporomandibular disorders patients and asymptomatic subjects: a systematic review and meta-analysis
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Pigozzi, Lucas Bozzetti, Pereira, Duziene Denardini, Pattussi, Marcos Pascoal, Moret-Tatay, Carmen, Irigaray, Tatiana Quarti, Weber, João Batista Blessmann, Grossi, Patrícia Krieger, and Grossi, Márcio Lima
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- 2021
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29. The Drosophila simulans Genome Lacks the crystal-Stellate System
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Anna De Grassi, Patrizia Tritto, Valeria Palumbo, Maria Pia Bozzetti, and Maria Francesca Berloco
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Drosophila ,heterochromatin ,cry-Ste ,evolution ,Cytology ,QH573-671 - Abstract
The cry-Ste system is a genetic interaction system between heterochromatin and euchromatin in Drosophila melanogaster, regulated via the piRNA pathway. Deregulation of this system leads to meiotic defects and male sterility. Although the cry-Ste system is peculiar to D. melanogaster, ancestors of Ste and Su(Ste) elements are present in the three closely related species, D. simulans, D. sechellia, and D. mauritiana. The birth, evolution, and maintenance of this genetic system in Drosophila melanogaster are of interest. We investigate the presence of sequences homologous to cry and Ste elements in the simulans complex and describe their chromosomal distribution. The organization and expression of cry- and Ste-like sequences were further characterized in the D. simulans genome. Our results allow us to conclude that the cry-Ste genetic interaction system is absent in the D. simulans genome.
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- 2022
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30. Jugular Foramen Metastasis from Lung Cancer: A Case of 'A Mass without His Syndrome'
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Giovanni Ciavarro, Francesca Bozzetti, and Maurizio Falcioni
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Otorhinolaryngology ,RF1-547 - Published
- 2019
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31. TBC1D32 variants disrupt retinal ciliogenesis and cause retinitis pigmentosa
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Bocquet, Béatrice, primary, Borday, Caroline, additional, Erkilic, Nejla, additional, Mamaeva, Daria, additional, Donval, Alicia, additional, Masson-Garcia, Christel, additional, Parain, Karine, additional, Kaminska, Karolina, additional, Quinodoz, Mathieu, additional, Perea-Romero, Irene, additional, Garcia-Garcia, Gema, additional, Jimenez-Medina, Carla, additional, Boukhaddaoui, Hassan, additional, Coget, Arthur, additional, Leboucq, Nicolas, additional, Calzetti, Giacomo, additional, Gandolfi, Stefano A., additional, Percesepe, Antonio, additional, Barili, Valeria, additional, Uliana, Vera, additional, Delsante, Marco, additional, Bozzetti, Francesca, additional, Scholl, Hendrik P.N., additional, Corton, Marta, additional, Ayuso, Carmen, additional, Millan, Jose M., additional, Rivolta, Carlo, additional, Meunier, Isabelle, additional, Perron, Muriel, additional, and Kalatzis, Vasiliki, additional
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- 2023
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32. A case of external auditory canal osteoma complicated with cholesteatoma, mastoiditis, labyrinthitis and internal auditory canal pachymeningitis
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I. Iaccarino, F. Bozzetti, L. O. Piccioni, and M. Falcioni
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Otorhinolaryngology ,RF1-547 - Published
- 2019
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33. Predictive value of skeletal muscle mass for immunotherapy with nivolumab in non‐small cell lung cancer patients: A 'hypothesis‐generator' preliminary report
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Alessio Cortellini, Lucilla Verna, Giampiero Porzio, Federico Bozzetti, Pierpaolo Palumbo, Carlo Masciocchi, Katia Cannita, Alessandro Parisi, Davide Brocco, Nicola Tinari, and Corrado Ficorella
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Immunotherapy ,nivolumab ,NSCLC ,sarcopenia ,skeletal muscle ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
Sarcopenia represents one of the hallmarks of all chronic disease, including non‐small cell lung cancer (NSCLC). A computed tomography scan is an easy modality to estimate the skeletal muscle mass through cross‐sectional image analysis at the level of the third lumbar vertebra (L3). Baseline skeletal muscle mass (SMM) was evaluated using gender‐specific cutoffs for skeletal muscle index in NSCLC patients administered immunotherapy with nivolumab to evaluate its possible correlations with clinical outcomes. From April 2015 to August 2018, 23 stage IV NSCLC patients were eligible for image analysis. Nine patients (39.1%) had low SMM. Among patients with baseline low and non‐low SMM, median progression free survival was 3.1 and 3.8 months, respectively (P = 0.0560), while median overall survival was 4.1 and 13 months, respectively (P = 0.2866). This hypothesis‐generating preliminary report offers the opportunity to speculate about the negative influence of sarcopenia on immune response. In our opinion, nutritional status could affect the clinical outcomes of immunotherapy, even if we cannot make definitive conclusions here. Further studies on the topic are required.
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- 2019
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34. Primary emissions versus secondary formation of fine particulate matter in the most polluted city (Shijiazhuang) in North China
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R.-J. Huang, Y. Wang, J. Cao, C. Lin, J. Duan, Q. Chen, Y. Li, Y. Gu, J. Yan, W. Xu, R. Fröhlich, F. Canonaco, C. Bozzetti, J. Ovadnevaite, D. Ceburnis, M. R. Canagaratna, J. Jayne, D. R. Worsnop, I. El-Haddad, A. S. H. Prévôt, and C. D. O'Dowd
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Physics ,QC1-999 ,Chemistry ,QD1-999 - Abstract
Particulate matter (PM) pollution is a severe environmental problem in the Beijing–Tianjin–Hebei (BTH) region in North China. PM studies have been conducted extensively in Beijing, but the chemical composition, sources, and atmospheric processes of PM are still relatively less known in nearby Tianjin and Hebei. In this study, fine PM in urban Shijiazhuang (the capital of Hebei Province) was characterized using an Aerodyne quadrupole aerosol chemical speciation monitor (Q-ACSM) from 11 January to 18 February in 2014. The average mass concentration of non-refractory submicron PM (diameter µm, NR-PM1) was 178±101 µg m−3, and it was composed of 50 % organic aerosol (OA), 21 % sulfate, 12 % nitrate, 11 % ammonium, and 6 % chloride. Using the multilinear engine (ME-2) receptor model, five OA sources were identified and quantified, including hydrocarbon-like OA from vehicle emissions (HOA, 13 %), cooking OA (COA, 16 %), biomass burning OA (BBOA, 17 %), coal combustion OA (CCOA, 27 %), and oxygenated OA (OOA, 27 %). We found that secondary formation contributed substantially to PM in episodic events, whereas primary emissions were dominant (most significant) on average. The episodic events with the highest NR-PM1 mass range of 300–360 µg m−3 were comprised of 55 % of secondary species. On the contrary, a campaign-average low OOA fraction (27 %) in OA indicated the importance of primary emissions, and a low sulfur oxidation degree (FSO4) of 0.18 even at RH >90 % hinted at insufficient oxidation. These results suggested that in Shijiazhuang in wintertime fine PM was mostly from primary emissions without sufficient atmospheric aging, indicating opportunities for air quality improvement by mitigating direct emissions. In addition, secondary inorganic and organic (OOA) species dominated in pollution events with high-RH conditions, most likely due to enhanced aqueous-phase chemistry, whereas primary organic aerosol (POA) dominated in pollution events with low-RH and stagnant conditions. These results also highlighted the importance of meteorological conditions for PM pollution in this highly polluted city in North China.
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- 2019
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35. Helical axis analysis to quantify humeral kinematics during shoulder rotation
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Corrado Cescon, Marco Barbero, Marco Conti, Francesco Bozzetti, and Jeremy Lewis
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helical axis ,shoulder ,joint ,kinematics ,Biotechnology ,TP248.13-248.65 ,Physiology ,QP1-981 - Abstract
Information pertaining to the helical axis during humeral kinematics during shoulder rotation may be of benefit to better understand conditions such as shoulder instability. The aim of this study is to quantify the behavior of humeral rotations using helical axis (HA) parameters in three different conditions. A total of 19 people without shoulder symptoms participated in the experiment. Shoulder kinematics was measured with an optoelectric motion capture system. The subjects performed three different full range rotations of the shoulder. The shoulder movements were analyzed with the HA technique. Four parameters were extracted from the HA of the shoulder during three different full-range rotations: range of movement (RoM), mean angle (MA), axis dispersion (MDD), and distance of their center from the shoulder (D). No significant differences were observed in the RoM for each condition between left and right side. The MA of the axis was significantly lower on the right side compared to the left in each of the three conditions. The MDD was also lower for the right side compared to the left side in each of the three conditions.The four parameters proposed for the analysis of shoulder kinematics showed to be promising indicators of shoulder instability.
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- 2019
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36. ESPEN practical guideline: Home parenteral nutrition
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Loris Pironi, Kurt Boeykens, Federico Bozzetti, Francisca Joly, Stanislaw Klek, Simon Lal, Marek Lichota, Stefan Mühlebach, Andre Van Gossum, Geert Wanten, Carolyn Wheatley, and Stephan C. Bischoff
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All institutes and research themes of the Radboud University Medical Center ,lnfectious Diseases and Global Health Radboud Institute for Health Sciences [Radboudumc 4] ,Nutrition and Dietetics ,Critical Care and Intensive Care Medicine - Abstract
Contains fulltext : 290886.pdf (Publisher’s version ) (Closed access) This guideline will inform physicians, nurses, dieticians, pharmacists, caregivers and other home parenteral nutrition (HPN) providers, as well as healthcare administrators and policy makers, about appropriate and safe HPN provision. This guideline will also inform patients requiring HPN. The guideline is based on previous published guidelines and provides an update of current evidence and expert opinion; it consists of 71 recommendations that address the indications for HPN, central venous access device (CVAD) and infusion pump, infusion catheter and CVAD site care, nutritional admixtures, program monitoring and management. Meta-analyses, systematic reviews and single clinical trials based on clinical questions were searched according to the PICO format. The evidence was evaluated and used to develop clinical recommendations implementing Scottish Intercollegiate Guidelines Network methodology. The guideline was commissioned and financially supported by ESPEN and members of the guideline group were selected by ESPEN.
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- 2023
37. High contributions of vehicular emissions to ammonia in three European cities derived from mobile measurements
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Elser, Miriam, El-Haddad, Imad, Maasikmets, Marek, Bozzetti, Carlo, Wolf, Robert, Ciarelli, Giancarlo, Slowik, Jay G., Richter, Rene, Teinemaa, Erik, Hüglin, Christoph, Baltensperger, Urs, and Prévôt, André S.H.
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- 2018
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38. RESUMOS DE DISSERTAÇÕES DE MESTRADO DEFENDIDAS EM 2004
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ALEXANDRE BOZZETTI BALDI
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Medicine - Abstract
PROGRAMA DE PÓS-GRADUAÇÃO EM MEDICINA: CARDIOLOGIA
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- 2020
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39. Advanced source apportionment of carbonaceous aerosols by coupling offline AMS and radiocarbon size-segregated measurements over a nearly 2-year period
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A. Vlachou, K. R. Daellenbach, C. Bozzetti, B. Chazeau, G. A. Salazar, S. Szidat, J.-L. Jaffrezo, C. Hueglin, U. Baltensperger, I. E. Haddad, and A. S. H. Prévôt
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Physics ,QC1-999 ,Chemistry ,QD1-999 - Abstract
Carbonaceous aerosols are related to adverse human health effects. Therefore, identification of their sources and analysis of their chemical composition is important. The offline AMS (aerosol mass spectrometer) technique offers quantitative separation of organic aerosol (OA) factors which can be related to major OA sources, either primary or secondary. While primary OA can be more clearly separated into sources, secondary (SOA) source apportionment is more challenging because different sources – anthropogenic or natural, fossil or non-fossil – can yield similar highly oxygenated mass spectra. Radiocarbon measurements provide unequivocal separation between fossil and non-fossil sources of carbon. Here we coupled these two offline methods and analysed the OA and organic carbon (OC) of different size fractions (particulate matter below 10 and 2.5 µm – PM10 and PM2.5, respectively) from the Alpine valley of Magadino (Switzerland) during the years 2013 and 2014 (219 samples). The combination of the techniques gave further insight into the characteristics of secondary OC (SOC) which was rather based on the type of SOC precursor and not on the volatility or the oxidation state of OC, as typically considered. Out of the primary sources separated in this study, biomass burning OC was the dominant one in winter, with average concentrations of 5.36 ± 2.64 µg m−3 for PM10 and 3.83 ± 1.81 µg m−3 for PM2.5, indicating that wood combustion particles were predominantly generated in the fine mode. The additional information from the size-segregated measurements revealed a primary sulfur-containing factor, mainly fossil, detected in the coarse size fraction and related to non-exhaust traffic emissions with a yearly average PM10 (PM2.5) concentration of 0.20 ± 0.24 µg m−3 (0.05 ± 0.04 µg m−3). A primary biological OC (PBOC) was also detected in the coarse mode peaking in spring and summer with a yearly average PM10 (PM2.5) concentration of 0.79 ± 0.31 µg m−3 (0.24 ± 0.20 µg m−3). The secondary OC was separated into two oxygenated, non-fossil OC factors which were identified based on their seasonal variability (i.e. summer and winter oxygenated organic carbon, OOC) and a third anthropogenic OOC factor which correlated with fossil OC mainly peaking in winter and spring, contributing on average 13 % ± 7 % (10 % ± 9 %) to the total OC in PM10 (PM2.5). The winter OOC was also connected to anthropogenic sources, contributing on average 13 % ± 13 % (6 % ± 6 %) to the total OC in PM10 (PM2.5). The summer OOC (SOOC), stemming from oxidation of biogenic emissions, was more pronounced in the fine mode, contributing on average 43 % ± 12 % (75 % ± 44 %) to the total OC in PM10 (PM2.5). In total the non-fossil OC significantly dominated the fossil OC throughout all seasons, by contributing on average 75 % ± 24 % to the total OC. The results also suggested that during the cold period the prevailing source was residential biomass burning while during the warm period primary biological sources and secondary organic aerosol from the oxidation of biogenic emissions became important. However, SOC was also formed by aged fossil fuel combustion emissions not only in summer but also during the rest of the year.
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- 2018
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40. Large contribution of fossil fuel derived secondary organic carbon to water soluble organic aerosols in winter haze in China
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Y.-L. Zhang, I. El-Haddad, R.-J. Huang, K.-F. Ho, J.-J. Cao, Y. Han, P. Zotter, C. Bozzetti, K. R. Daellenbach, J. G. Slowik, G. Salazar, A. S. H. Prévôt, and S. Szidat
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Physics ,QC1-999 ,Chemistry ,QD1-999 - Abstract
Water-soluble organic carbon (WSOC) is a large fraction of organic aerosols (OA) globally and has significant impacts on climate and human health. The sources of WSOC remain very uncertain in polluted regions. Here we present a quantitative source apportionment of WSOC, isolated from aerosols in China using radiocarbon (14C) and offline high-resolution time-of-flight aerosol mass spectrometer measurements. Fossil emissions on average accounted for 32–47 % of WSOC. Secondary organic carbon (SOC) dominated both the non-fossil and fossil derived WSOC, highlighting the importance of secondary formation to WSOC in severe winter haze episodes. Contributions from fossil emissions to SOC were 61 ± 4 and 50 ± 9 % in Shanghai and Beijing, respectively, significantly larger than those in Guangzhou (36 ± 9 %) and Xi'an (26 ± 9 %). The most important primary sources were biomass burning emissions, contributing 17–26 % of WSOC. The remaining primary sources such as coal combustion, cooking and traffic were generally very small but not negligible contributors, as coal combustion contribution could exceed 10 %. Taken together with earlier 14C source apportionment studies in urban, rural, semi-urban and background regions in Asia, Europe and the USA, we demonstrated a dominant contribution of non-fossil emissions (i.e., 75 ± 11 %) to WSOC aerosols in the Northern Hemisphere; however, the fossil fraction is substantially larger in aerosols from East Asia and the eastern Asian pollution outflow, especially during winter, due to increasing coal combustion. Inclusion of our findings can improve a modelling of effects of WSOC aerosols on climate, atmospheric chemistry and public health.
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- 2018
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41. Improved source apportionment of organic aerosols in complex urban air pollution using the multilinear engine (ME-2)
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Q. Zhu, X.-F. Huang, L.-M. Cao, L.-T. Wei, B. Zhang, L.-Y. He, M. Elser, F. Canonaco, J. G. Slowik, C. Bozzetti, I. El-Haddad, and A. S. H. Prévôt
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Environmental engineering ,TA170-171 ,Earthwork. Foundations ,TA715-787 - Abstract
Organic aerosols (OAs), which consist of thousands of complex compounds emitted from various sources, constitute one of the major components of fine particulate matter. The traditional positive matrix factorization (PMF) method often apportions aerosol mass spectrometer (AMS) organic datasets into less meaningful or mixed factors, especially in complex urban cases. In this study, an improved source apportionment method using a bilinear model of the multilinear engine (ME-2) was applied to OAs collected during the heavily polluted season from two Chinese megacities located in the north and south with an Aerodyne high-resolution aerosol mass spectrometer (HR-ToF-AMS). We applied a rather novel procedure for utilization of prior information and selecting optimal solutions, which does not necessarily depend on other studies. Ultimately, six reasonable factors were clearly resolved and quantified for both sites by constraining one or more factors: hydrocarbon-like OA (HOA), cooking-related OA (COA), biomass burning OA (BBOA), coal combustion (CCOA), less-oxidized oxygenated OA (LO-OOA) and more-oxidized oxygenated OA (MO-OOA). In comparison, the traditional PMF method could not effectively resolve the appropriate factors, e.g., BBOA and CCOA, in the solutions. Moreover, coal combustion and traffic emissions were determined to be primarily responsible for the concentrations of PAHs and BC, respectively, through the regression analyses of the ME-2 results.
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- 2018
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42. Long-term chemical analysis and organic aerosol source apportionment at nine sites in central Europe: source identification and uncertainty assessment
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K. R. Daellenbach, G. Stefenelli, C. Bozzetti, A. Vlachou, P. Fermo, R. Gonzalez, A. Piazzalunga, C. Colombi, F. Canonaco, C. Hueglin, A. Kasper-Giebl, J.-L. Jaffrezo, F. Bianchi, J. G. Slowik, U. Baltensperger, I. El-Haddad, and A. S. H. Prévôt
- Subjects
Physics ,QC1-999 ,Chemistry ,QD1-999 - Abstract
Long-term monitoring of organic aerosol is important for epidemiological studies, validation of atmospheric models, and air quality management. In this study, we apply a recently developed filter-based offline methodology using an aerosol mass spectrometer (AMS) to investigate the regional and seasonal differences of contributing organic aerosol sources. We present offline AMS measurements for particulate matter smaller than 10 µm at nine stations in central Europe with different exposure characteristics for the entire year of 2013 (819 samples). The focus of this study is a detailed source apportionment analysis (using positive matrix factorization, PMF) including in-depth assessment of the related uncertainties. Primary organic aerosol (POA) is separated in three components: hydrocarbon-like OA related to traffic emissions (HOA), cooking OA (COA), and biomass burning OA (BBOA). We observe enhanced production of secondary organic aerosol (SOA) in summer, following the increase in biogenic emissions with temperature (summer oxygenated OA, SOOA). In addition, a SOA component was extracted that correlated with an anthropogenic secondary inorganic species that is dominant in winter (winter oxygenated OA, WOOA). A factor (sulfur-containing organic, SC-OA) explaining sulfur-containing fragments (CH3SO2+), which has an event-driven temporal behaviour, was also identified. The relative yearly average factor contributions range from 4 to 14 % for HOA, from 3 to 11 % for COA, from 11 to 59 % for BBOA, from 5 to 23 % for SC-OA, from 14 to 27 % for WOOA, and from 15 to 38 % for SOOA. The uncertainty of the relative average factor contribution lies between 2 and 12 % of OA. At the sites north of the alpine crest, the sum of HOA, COA, and BBOA (POA) contributes less to OA (POA / OA = 0.3) than at the southern alpine valley sites (0.6). BBOA is the main contributor to POA with 87 % in alpine valleys and 42 % north of the alpine crest. Furthermore, the influence of primary biological particles (PBOAs), not resolved by PMF, is estimated and could contribute significantly to OA in PM10.
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- 2017
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43. Neurologic sequelae of COVID-19 are determined by immunologic imprinting from previous coronaviruses
- Author
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Spatola, Marianna, primary, Nziza, Nadège, additional, Jung, Wonyeong, additional, Deng, Yixiang, additional, Yuan, Dansu, additional, Dinoto, Alessandro, additional, Bozzetti, Silvia, additional, Chiodega, Vanessa, additional, Ferrari, Sergio, additional, Lauffenburger, Douglas A, additional, Mariotto, Sara, additional, and Alter, Galit, additional
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- 2023
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44. Expression of Transposable Elements in the Brain of the Drosophila melanogaster Model for Fragile X Syndrome
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De Donno, Maria Dolores, primary, Puricella, Antonietta, additional, D’Attis, Simona, additional, Specchia, Valeria, additional, and Bozzetti, Maria Pia, additional
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- 2023
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45. Chemical composition, sources and secondary processes of aerosols in Baoji city of northwest China
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Wang, Y.C., Huang, R.-J., Ni, H.Y., Chen, Y., Wang, Q.Y., Li, G.H., Tie, X.X., Shen, Z.X., Huang, Y., Liu, S.X., Dong, W.M., Xue, P., Fröhlich, R., Canonaco, F., Elser, M., Daellenbach, K.R., Bozzetti, C., El Haddad, I., Prévôt, A.S.H., Canagaratna, M.R., Worsnop, D.R., and Cao, J.J.
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- 2017
- Full Text
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46. Weighing the role of skeletal muscle mass and muscle density in cancer patients receiving PD-1/PD-L1 checkpoint inhibitors: a multicenter real-life study
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Cortellini, Alessio, Bozzetti, Federico, Palumbo, Pierpaolo, Brocco, Davide, Di Marino, Pietro, Tinari, Nicola, De Tursi, Michele, Agostinelli, Veronica, Patruno, Leonardo, Valdesi, Cristina, Mereu, Manuela, Verna, Lucilla, Lanfiuti Baldi, Paola, Venditti, Olga, Cannita, Katia, Masciocchi, Carlo, Barile, Antonio, McQuade, Jennifer Leigh, Ficorella, Corrado, and Porzio, Giampiero
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- 2020
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47. Neurologic sequelae of COVID-19 are determined by immunologic imprinting from previous coronaviruses
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Marianna Spatola, Nadège Nziza, Wonyeong Jung, Yixiang Deng, Dansu Yuan, Alessandro Dinoto, Silvia Bozzetti, Vanessa Chiodega, Sergio Ferrari, Douglas A Lauffenburger, Sara Mariotto, and Galit Alter
- Subjects
Fc receptor ,SARS-CoV-2 ,antibody-mediated complement activation ,long-COVID ,Neurology (clinical) ,PASC - Abstract
Coronavirus disease 2019 (COVID-19), which is caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), remains a global public health emergency. Although SARS-CoV-2 is primarily a respiratory pathogen, extra-respiratory organs, including the central nervous system (CNS), can also be affected. Neurologic symptoms have been observed not only during acute SARS-CoV-2 infection, but also at distance from respiratory disease, also known as long-COVID or neurological post-acute sequelae of COVID-19 (neuroPASC). The pathogenesis of neuroPASC is not well understood, but hypotheses include SARS-CoV-2-induced immune dysfunctions, hormonal dysregulations, and persistence of SARS-CoV-2 reservoirs. In this prospective cohort study, we used a high throughput systems serology approach to dissect the humoral response to SARS-CoV-2 (and other common Coronaviruses - 229E, HKU1, NL63, OC43) in the serum and cerebrospinal fluid (CSF) from 112 infected individuals who developed (n = 18) or did not develop (n = 94) neuroPASC. Unique SARS-CoV-2 humoral profiles were observed in the CSF of neuroPASC, compared to serum responses. All antibody isotypes (IgG, IgM, IgA) and subclasses (IgA1-2; IgG1-4) were detected in serum, whereas CSF was characterized by focused IgG1 (and absence of IgM). These data argue in favor of compartmentalized brain-specific responses against SARS-CoV-2 through selective transfer of antibodies from the serum to the CSF across the blood-brain-barrier, rather than intrathecal synthesis, where more diversity in antibody classes/subclasses would be expected. Compared to individuals who did not develop post-acute complications following infection, individuals with neuroPASC had similar demographic features (median age 65 vs 66.5 years, respectively, p = 0.55; females 33% vs 44%, p = 0.52), but exhibited attenuated systemic antibody responses against SARS-CoV-2, characterized by decreased capacity to activate antibody-dependent complement deposition (ADCD), NK cell activation (ADNKA) and to bind Fcγ receptors. However, surprisingly, neuroPASC individuals showed significantly expanded antibody responses to other common Coronaviruses, including 229E, HKU1, NL63, and OC43. This biased humoral activation across coronaviruses was particularly enriched in neuroPASC individuals with poor outcome, suggesting an original antigenic sin (or immunologic imprinting), where pre-existing immune responses against related viruses shape the response to current infection, as a key prognostic marker of neuroPASC disease. Overall, these findings point to a pathogenic role for compromised anti-SARS-CoV-2 responses in the CSF, likely resulting in incomplete virus clearance from the brain and persistent neuroinflammation, in the development of post-acute neurologic complications of SARS-CoV-2 infection.
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- 2023
48. Surgical Navigation in Mandibular Reconstruction: Accuracy Evaluation of an Innovative Protocol
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Sozzi, D, Filippi, A, Canzi, G, De Ponti, E, Bozzetti, A, Novelli, G, Sozzi, Davide, Filippi, Andrea, Canzi, Gabriele, De Ponti, Elena, Bozzetti, Alberto, Novelli, Giorgio, Sozzi, D, Filippi, A, Canzi, G, De Ponti, E, Bozzetti, A, Novelli, G, Sozzi, Davide, Filippi, Andrea, Canzi, Gabriele, De Ponti, Elena, Bozzetti, Alberto, and Novelli, Giorgio
- Abstract
Aim: the purpose of this work is to present an innovative protocol for virtual planning and surgical navigation in post-oncological mandibular reconstruction through fibula free flap. In order to analyze its applicability, an evaluation of accuracy for the surgical protocol has been performed. Methods: 21 patients surgically treated for mandibular neoplasm have been included in the analysis. The Brainlab Vector Vision 3.0® software for surgical navigation has been used for preoperative surgical planning and intra-operative navigation. A post-operative accuracy evaluation has been performed matching the position of mandibular landmarks between pre-operative and post-operative CT scans. Results: the maximal discrepancy observed was included between −3.4 mm and +3.2 mm, assuming negative values for under correction and positive values for overcorrection. An average grade of accuracy included between 0.06 ± 0.58 mm and 0.43 ± 0.68 mm has been observed for every mandibular landmark examined, except for mandibular angles that showed a mean discrepancy value included between 1.36 ± 1.73 mm and 1.46 ± 1.02 mm when compared to preoperative measurements. Conclusion: a satisfying level of accuracy has been observed in the protocol presented, which appears to be more versatile if compared to closed custom-made systems. The technique described may represent a valid option for selected patients, but it cannot be considered for routine activity because of the complexity of the method, the mobility of the jaw, the necessity of surgical navigator and the long surgical learning curve that is required.
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- 2022
49. The burden of facial trauma on mortality in patients with multiple injuries: A single-center analysis of 1862 motorcycle accidents
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Canzi, G, De Ponti, E, Filippi, A, Bozzetti, A, Sozzi, D, Novelli, G, Canzi G., De Ponti E., Filippi A., Bozzetti A., Sozzi D., Novelli G., Canzi, G, De Ponti, E, Filippi, A, Bozzetti, A, Sozzi, D, Novelli, G, Canzi G., De Ponti E., Filippi A., Bozzetti A., Sozzi D., and Novelli G.
- Abstract
The study aims to assess the influence on mortality of motorcycle road accidents, caused by injuries to the head, chest, abdomen, face, skin, pelvis and extremities. Road motorcycle accidents consecutively admitted to Level I Trauma Center were retrospectively analyzed. Each body site involvement was classified through Abbreviated Injury Score (AIS-98), and Comprehensive Facial Injury (CFI) score; Injury Severity Score (ISS) was also calculated. The data collected were subjected to a descriptive analysis and inferential statistic, with uni- and multivariate analysis; mortality was the main outcome examined. 1862 patients were studied. Limbs (53.9%) and Head (53.8%) are the most involved body site, facial trauma regards 19.4% of the sample. Only 4.4% of Facial injuries occurred as isolated, 71.6% were associated to Head involvement. The overall mortality was 4.6% and 80.0% of dead patients were affected by Head injury. Multivariate analysis shows that head (OR=3.06, p <0.0001), thoracic (OR=1.82, p <0.0001) and abdominal trauma (OR=1.41, p =0.019) are predicting the risk of death. Facial trauma does not directly influence mortality and, however severe and distracting it may be, becomes secondary to the management of frequently associated brain injuries. Severity scores targeted to the risk of death, such as AIS and AIS-derived, are ineffective in describing the true characteristics of facial injuries. The CFI score has been shown to predict the weight of surgical treatment and the outcome of the hospital stay, therefore its use is recommended.
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- 2022
50. “6 Anatomical Landmarks” Technique for Satisfactory Free-Hand Orbital Reconstruction With Standard Preformed Titanium Mesh
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Canzi, G, Corradi, F, Novelli, G, Bozzetti, A, Sozzi, D, Canzi, Gabriele, Corradi, Federica, Novelli, Giorgio, Bozzetti, Alberto, Sozzi, Davide, Canzi, G, Corradi, F, Novelli, G, Bozzetti, A, Sozzi, D, Canzi, Gabriele, Corradi, Federica, Novelli, Giorgio, Bozzetti, Alberto, and Sozzi, Davide
- Abstract
Objective: Resolution of clinical signs and symptoms following orbital fractures depends on the accurate restoration of the orbital volume. Computer-Assisted procedures and Patient Specific Implants represent modern solutions, but they require additional resources. A more reproducible option is the use of standard preformed titanium meshes, widely available and cheaper; with their use quality of results is proportional to the accuracy with which they are positioned. This work identifies 6 reproducible and constant anatomical landmarks, as an intraoperative guide for the precise positioning of titanium preformed meshes. Methods: 90 patients treated at the Maxillofacial Surgery Department, Niguarda Trauma Center, Milan, for unilateral orbital reconstruction (January 2012 to December 2018), were studied. In all cases reconstruction was performed respecting the 6 proposed anatomical landmarks. The outcomes analyzed are: post-operative CT adherence to the 6 anatomical markers and symmetry achieved respect to controlateral orbit; number/year of re-interventions and duration of surgery; resolution of clinical defects (at least 12-months follow-up); incidence of complications. Results: Satisfactory results were obtained in terms of restoration of orbital size, shape and volume. Clinical defects early recovered with a low incidence of complications and re-interventions. Operating times and radiological accuracy have shown a progressive improvement during years of application of this technique. Conclusions: The proposed "6 anatomical landmarks" is an easy free-hand technique that allows everyone to obtain high levels of reconstructive accuracy and it should be a skill of all surgeons who deal with orbital reconstruction in daily clinical activity.
- Published
- 2022
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