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4 results on '"Chen, Yuanhuang"'

1. Multiplexed mapping of the interactome of GPCRs with receptor activity-modifying proteins

Author

Kotliar, Ilana B., Bendes, Annika, Dahl, Leo, Chen, Yuanhuang, Saarinen, Marcus, Ceraudo, Emilie, Dodig-Crnkovic, Tea, Uhlén, Mathias, Svenningsson, Per, Schwenk, Jochen M., Sakmar, Thomas P., Kotliar, Ilana B., Bendes, Annika, Dahl, Leo, Chen, Yuanhuang, Saarinen, Marcus, Ceraudo, Emilie, Dodig-Crnkovic, Tea, Uhlén, Mathias, Svenningsson, Per, Schwenk, Jochen M., and Sakmar, Thomas P.

Abstract

Receptor activity-modifying proteins (RAMPs) form complexes with G protein-coupled receptors (GPCRs) and may regulate their cellular trafficking and pharmacology. RAMP interactions have been identified for about 50 GPCRs, but only a few GPCR-RAMP complexes have been studied in detail. To elucidate a comprehensive GPCR-RAMP interactome, we created a library of 215 dual epitope-tagged (DuET) GPCRs representing all GPCR subfamilies and coexpressed each GPCR with each of the three RAMPs. Screening the GPCR-RAMP pairs with customized multiplexed suspension bead array (SBA) immunoassays, we identified 122 GPCRs that showed strong evidence for interaction with at least one RAMP. We screened for interactions in three cell lines and found 23 endogenously expressed GPCRs that formed complexes with RAMPs. Mapping the GPCR-RAMP interactome expands the current system-wide functional characterization of RAMP-interacting GPCRs to inform the design of selective therapeutics targeting GPCR-RAMP complexes., QC 20240820

Published
2024
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3. Multiplexed mapping of the interactome of GPCRs with receptor activity-modifying proteins.

Author

Kotliar IB, Bendes A, Dahl L, Chen Y, Saarinen M, Ceraudo E, Dodig-Crnković T, Uhlén M, Svenningsson P, Schwenk JM, and Sakmar TP

Subjects
Humans, Protein Interaction Mapping methods, HEK293 Cells, Protein Interaction Maps, Receptors, G-Protein-Coupled metabolism, Receptor Activity-Modifying Proteins metabolism, Protein Binding
Abstract

Receptor activity-modifying proteins (RAMPs) form complexes with G protein-coupled receptors (GPCRs) and may regulate their cellular trafficking and pharmacology. RAMP interactions have been identified for about 50 GPCRs, but only a few GPCR-RAMP complexes have been studied in detail. To elucidate a comprehensive GPCR-RAMP interactome, we created a library of 215 dual epitope-tagged (DuET) GPCRs representing all GPCR subfamilies and coexpressed each GPCR with each of the three RAMPs. Screening the GPCR-RAMP pairs with customized multiplexed suspension bead array (SBA) immunoassays, we identified 122 GPCRs that showed strong evidence for interaction with at least one RAMP. We screened for interactions in three cell lines and found 23 endogenously expressed GPCRs that formed complexes with RAMPs. Mapping the GPCR-RAMP interactome expands the current system-wide functional characterization of RAMP-interacting GPCRs to inform the design of selective therapeutics targeting GPCR-RAMP complexes.

Published
2024
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4. Expanding the GPCR-RAMP interactome.

Author

Kotliar IB, Bendes A, Dahl L, Chen Y, Saarinen M, Ceraudo E, Dodig-Crnković T, Uhle'n M, Svenningsson P, Schwenk JM, and Sakmar TP

Abstract

Receptor activity-modifying proteins (RAMPs) can form complexes with G protein-coupled receptors (GPCRs) and regulate their cellular trafficking and pharmacology. RAMP interactions have been identified for about 50 GPCRs, but only a few GPCR-RAMP complexes have been studied in detail. To elucidate a complete interactome between GPCRs and the three RAMPs, we developed a customized library of 215 Dual Epitope-Tagged (DuET) GPCRs representing all GPCR subfamilies. Using a multiplexed suspension bead array (SBA) assay, we identified 122 GPCRs that showed strong evidence for interaction with at least one RAMP. We screened for native interactions in three cell lines and found 23 GPCRs that formed complexes with RAMPs. Mapping the GPCR-RAMP interactome expands the current system-wide functional characterization of RAMP-interacting GPCRs to inform the design of selective GPCR-targeted therapeutics., Competing Interests: M.U. is a cofounder of Atlas Antibodies AB, the commercial distributor of some of the Abs used in this study. J.M.S. acknowledges a relationship with Atlas Antibodies AB. J.M.S. has, unrelated to this study, conducted contract research with Luminex Corp., received travel and accommodation expenses from Luminex Corp., and received speaker fees from Roche Diagnostics. The other authors declare that they have no competing interests.

Published
2023
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