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This work investigated the implications that mediation in a Science Center can promote in initial education of future physics teachers. The qualitative research was carried out with undergraduate who act as mediators in a Science Center integrated with the formative activities of a Federal University in the State of Minas Gerais, and semi-structured interviews were used as a data collection instrument. The interviews were analyzed qualitatively, and it was possible to notice that the performance in the non-formal teaching space has direct implications in the performance of the undergraduates in the classroom, either during the internship period or during their training, in addition to contributing to a broader understanding of teaching and learning processes.