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15 results on '"Di Giorgio M"'

1. Biological Dosimetry Intercomparison Exercise: An Evaluation of Triage and Routine Mode Results by Robust Methods

Author

Di Giorgio, M., Barquinero, J. F., Vallerga, M. B., Radl, A., Taja, M. R., Seoane, A., De Luca, J., Oliveira, M. Stuck, Valdivia, P., Lima, O. García, Lamadrid, A., Mesa, J. González, Aguilera, I. Romero, Cardoso, T. Mandina, Carvajal, Y. C. Guerrero, Maldonado, C. Arceo, Espinoza, M. E., Martínez-López, W., Méndez-Acuña, L., Di Tomaso, M. V., Roy, L., Lindholm, C., Romm, H., Güçlü, I., and Lloyd, D. C.

Published
2011
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3. BIODOSIMETRY AND BIODOSIMETRY NETWORKS FOR MANAGING RADIATION EMERGENCY

Author

Kulka, U., Wojcik, Andrzej, Di Giorgio, M., Wilkins, R., Suto, Y., Jang, S., Quing-Jie, L., Jiaxiang, L., Ainsbury, E., Woda, C., Roy, L., Li, C., Lloyd, D., Carr, Z., Kulka, U., Wojcik, Andrzej, Di Giorgio, M., Wilkins, R., Suto, Y., Jang, S., Quing-Jie, L., Jiaxiang, L., Ainsbury, E., Woda, C., Roy, L., Li, C., Lloyd, D., and Carr, Z.

Abstract

Biological dosimetry enables individual dose reconstruction in the case of unclear or inconsistent radiation exposure situations, especially when a direct measurement of ionizing radiation is not or is no longer possible. To be prepared for large-scale radiological incidents, networking between well-trained laboratories has been identified as a useful approach for provision of the fast and trustworthy dose assessments needed in such circumstances. To this end, various biodosimetry laboratories worldwide have joined forces and set up regional and/or nationwide networks either on a formal or informal basis. Many of these laboratories are also a part of global networks such as those organized by World Health Organization, International Atomic Energy Agency or Global Health Security Initiative. In the present report, biodosimetry networks from different parts of the world are presented, and the partners, activities and cooperation actions are detailed. Moreover, guidance for situational application of tools used for individual dosimetry is given.

Published
2018
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4. Liver transplantation for early-stage hepatocellular carcinoma: An intention to treat analysis

Author

Dezza, M, Corno, V, Lucianetti, A, Zambelli, M, Guizzetti, M, Pinelli, D, Sonzogni, A, Gaffuri, G, Vigano, R, Pasulo, L, Di Giorgio, M, Luca, M, Colpani, M, Verga, G, Fagiuoli, S, Colledan, M, Dezza MC, Corno V, Lucianetti A, Zambelli M, Guizzetti M, Pinelli D, Sonzogni A, Gaffuri G, Vigano R, Pasulo L, Di Giorgio M, Luca MG, Colpani M, Verga G, Fagiuoli S, Colledan M, Dezza, M, Corno, V, Lucianetti, A, Zambelli, M, Guizzetti, M, Pinelli, D, Sonzogni, A, Gaffuri, G, Vigano, R, Pasulo, L, Di Giorgio, M, Luca, M, Colpani, M, Verga, G, Fagiuoli, S, Colledan, M, Dezza MC, Corno V, Lucianetti A, Zambelli M, Guizzetti M, Pinelli D, Sonzogni A, Gaffuri G, Vigano R, Pasulo L, Di Giorgio M, Luca MG, Colpani M, Verga G, Fagiuoli S, and Colledan M

Published
2007

8. TERAPIA DELL'ANDROPAUSA

Author

Romanelli, Francesco, Fattorini, Giorgio, Latini, M, DI GIORGIO, M. R., Sofia, A, and Lenzi, Andrea

Published
2006

10. Assessment of individual radiosensitivity in human lymphocytes of cancer patients and its correlation with adverse side effects to radiation therapy

Author

Di Giorgio, M, Bonomi, M, Busto, E, Mairal, L, Menendez, P, Roth, B, Sardi, M, Taja, M R, and Vallerga, M B

Subjects
Health Physics and Radiation Effects
Abstract

Background and purpose: Individual radiosensitivity is an inherent characteristic, associated with an increased reaction to ionizing radiation on the human body. Biological endpoints such as clonogenic survival, chromosome aberration formation and repair capacity of radiation-induced damage have been applied to evaluate individual radiosensitivity in vitro. 5%-7% of cancer patients develop adverse side effects to radiation therapy in normal tissues within the treatment field, which are referred as 'clinical radiation reactions' and include acute effects, late effects and cancer induction. It has been hypothesized that the occurrence and severity of these reactions are mainly influenced by genetic susceptibility to radiation. Additionally, the nature of the genetic disorders associated with hypersensitivity to radiotherapy suggests that DNA repair mechanisms are involved. Consequently, the characterization of DNA repair in lymphocytes through cytokinesis blocked micronucleus (MN) and alkaline single-cell micro gel electrophoresis (comet) assays could be suitable approaches to evaluate individual radiosensitivity in vitro. The MN assay is an established cytogenetic technique to evaluate intrinsic cell radiosensitivity in tumor cells and lymphocytes; comet assay is a sensitive and rapid method for measuring DNA damage and repair in individual cells. The aims of this study were: 1) To assess the in vitro radiosensitivity of peripheral blood lymphocytes from two groups of cancer patients (retrospectively and prospectively studied), using MN and comet assays, in comparison with the observed clinical response; and 2) To test the predictive potential of both techniques. Materials and methods: 38 cancer patients receiving radiation therapy were enrolled in this study. The tumor sites were: head and neck (n 25) and cervix (n = 13). Nineteen patients were evaluated about 6-18 month after radiotherapy (retrospective group) and 19 patients were evaluated prior, mid-way an of a treatment (prospective group). In the retrospective group, blood samples were irradiated in vitro with 0 (control) or 2 Gy and evaluated using MN test. Cytogenetic data were analyzed comparing expected MN frequencies (calibration curve from healthy donors) with values observed after in vitro irradiation. One over reactor was also evaluated trough comet assay. DNA damage and repair capacity were quantified by the tail moment. Lymphocytes of healthy individuals were used as reference sample. Cytogenetic data from the prospective group were analyzed using a mathematical model to evaluate the attenuation of the cytogenetic effect as a function of the time between a single exposure and blood sampling, estimating a cytogenetic recovery factor k. Results: In the retrospective evaluation, lymphocytes of 3 from 4 patients that had developed late reactions were significantly more radiosensitive than lymphocytes from the rest of the patients and normal donors. The individual cytogenetic response suggests a correlation with the maximum grade of late reaction (osteonecrosis, fibrosis and trismus). The patient additionally evaluated by comet assay showed reduced DNA repair capacity. In the prospective evaluation, factor k correlated with the individual radiosensitivity. Patients with low recovery from the cytogenetic effect (k tending to zero) developed late toxicity (fibrosis and actinic rectitis). Conclusions: MN and comet tests could be suitable predictive assays to evaluate individual radiosensitivity in vitro, contributing with the detection of patients that develop late adverse reactions. However, further studies are required to confirm the validity of these tests.

Published
2003

11. Intra- and inter-laboratory variation in the scoring of micronuclei and nucleoplasmic bridges in binucleated human lymphocytes Results of an international slide-scoring exercise by the HUMN project

Author

Fenech, M, Bonassi, S, Turner, J, Lando, C, Ceppi, M, Chang, WP, Holland, N, Kirsch-Volders, M, Zeiger, E, Bigatti, MP, Bolognesi, C, Cao, J, De Luca, G, Di Giorgio, M, Ferguson, LR, Fucic, A, Lima, OG, Hadjidekova, VV, Hrelia, P, Jaworska, A, Joksić, Gordana, Krishnaja, AP, Lee, TK, Martelli, A, McKay, MJ, Migliore, L, Mirkova, E, Muller, WU, Odagiri, Y, Orsiere, T, Scarfi, MR, Silva, MJ, Sofuni, T, Suralles, J, Trenta, G, Vorobtsova, I, Vral, A, Zijno, A, Fenech, M, Bonassi, S, Turner, J, Lando, C, Ceppi, M, Chang, WP, Holland, N, Kirsch-Volders, M, Zeiger, E, Bigatti, MP, Bolognesi, C, Cao, J, De Luca, G, Di Giorgio, M, Ferguson, LR, Fucic, A, Lima, OG, Hadjidekova, VV, Hrelia, P, Jaworska, A, Joksić, Gordana, Krishnaja, AP, Lee, TK, Martelli, A, McKay, MJ, Migliore, L, Mirkova, E, Muller, WU, Odagiri, Y, Orsiere, T, Scarfi, MR, Silva, MJ, Sofuni, T, Suralles, J, Trenta, G, Vorobtsova, I, Vral, A, and Zijno, A

Abstract

One of the objectives of the HUman MicroNucleus (HUMN) project is to identify the methodological variables that have an important impact on micronucleus (MN) or micronucleated (MNed) cell frequencies measured in human lymphocytes using the cytokinesis-block micronucleus assay, In a previous study we had shown that the scoring criteria used were likely to be an important variable. To determine the extent of residual variation when laboratories scored cells from the same cultures using the same set of standard scoring criteria, an inter-laboratory slide-scoring exercise was performed among 34 laboratories from 21 countries with a total of 51 slide scorers involved. The results of this StUdy show that even under these optimized conditions there is a great variation in the MN frequency or MNed cell frequency obtained by individual laboratories and scorers. All laboratories ranked correctly the MNed cell frequency in cells from cultures that were unirradiated, or exposed to 1 or 2 Gy of gamma rays. The study also estimated that the intra-scorer median coefficient of variation for duplicate MNed cell frequency scores is 29% for unexposed cultures and 14 and 11% for cells exposed to I and 2 Gy, respectively, These values can be used as a standard for quality or acceptability of data in future studies. Using a Poisson regression model it was estimated that radiation dose explained 67% of the variance, while staining method. cell sample, laboratory, and covariance explained 0.6, 0.3, 6.5. and 25.6% of the variance, respectively, leaving only 3.1% of the variance unexplained. As part of this exercise. nucleoplasmic bridges were also estimated by the laboratories however, inexperience in the use of this biomarker of chromosome rearrangement was reflected in the much greater heterogeneity in the data and the unexplained variation estimated by the Poisson model. The results of these studies indicate clearly that even after standardizing culture and scoring conditions it will be

Published
2003

12. Intra and inter-laboratory variation in the scoring of micronucici and nucleoplasmic bridges in binucleated human lymphocytes - Results of an international slide-scoring exercise by the HUMN project (vol 534, pg 45, 2002)

Author

Fenech, M, Bonassi, S, Turner, J, Lando, C, Ceppi, M, Chang, WP, Holland, N, Kirsch-Volders, M, Zeiger, E, Bigatti, MP, Bolognesi, C, Cao, J, De Luca, G, Di Giorgio, M, Ferguson, LR, Fucic, A, Lima, OG, Hadjidekova, VV, Hrelia, P, Jaworska, A, Joksić, Gordana, Krishnaja, AP, Lee, TK, Martelli, A, McKay, MJ, Migliore, L, Mirkova, E, Muller, WU, Odagiri, Y, Orsiere, T, Scarfi, MR, Silva, MJ, Sofuni, T, Surralles, J, Trenta, G, Vorobtsova, I, Vral, A, Zijno, A, Fenech, M, Bonassi, S, Turner, J, Lando, C, Ceppi, M, Chang, WP, Holland, N, Kirsch-Volders, M, Zeiger, E, Bigatti, MP, Bolognesi, C, Cao, J, De Luca, G, Di Giorgio, M, Ferguson, LR, Fucic, A, Lima, OG, Hadjidekova, VV, Hrelia, P, Jaworska, A, Joksić, Gordana, Krishnaja, AP, Lee, TK, Martelli, A, McKay, MJ, Migliore, L, Mirkova, E, Muller, WU, Odagiri, Y, Orsiere, T, Scarfi, MR, Silva, MJ, Sofuni, T, Surralles, J, Trenta, G, Vorobtsova, I, Vral, A, and Zijno, A

Published
2003

13. Erratum: Intra and inter-laboratory variation in the scoring of micronucici and nucleoplasmic bridges in binucleated human lymphocytes - Results of an international slide-scoring exercise by the HUMN project (vol 534, pg 45, 2002)

Author

Fenech, M, Bonassi, S, Turner, J, Lando, C, Ceppi, M, Chang, WP, Holland, N, Kirsch-Volders, M, Zeiger, E, Bigatti, MP, Bolognesi, C, Cao, J, De Luca, G, Di Giorgio, M, Ferguson, LR, Fucic, A, Lima, OG, Hadjidekova, VV, Hrelia, P, Jaworska, A, Joksić, Gordana, Krishnaja, AP, Lee, TK, Martelli, A, McKay, MJ, Migliore, L, Mirkova, E, Muller, WU, Odagiri, Y, Orsiere, T, Scarfi, MR, Silva, MJ, Sofuni, T, Surralles, J, Trenta, G, Vorobtsova, I, Vral, A, Zijno, A, Fenech, M, Bonassi, S, Turner, J, Lando, C, Ceppi, M, Chang, WP, Holland, N, Kirsch-Volders, M, Zeiger, E, Bigatti, MP, Bolognesi, C, Cao, J, De Luca, G, Di Giorgio, M, Ferguson, LR, Fucic, A, Lima, OG, Hadjidekova, VV, Hrelia, P, Jaworska, A, Joksić, Gordana, Krishnaja, AP, Lee, TK, Martelli, A, McKay, MJ, Migliore, L, Mirkova, E, Muller, WU, Odagiri, Y, Orsiere, T, Scarfi, MR, Silva, MJ, Sofuni, T, Surralles, J, Trenta, G, Vorobtsova, I, Vral, A, and Zijno, A

Published
2003

15. ESTUDIO COORDINADO DE PAISES DE AMERICA LATINA EN DOSIMETRIA CITOGENETICA.

Author

García, O., Manzano, J., Lamadrid, A., Di Giorgio, M., Nasazzi, N., Taja, M. R., Ferraro, M., Ramalho, A., Nascimiento, A. C., Sanhueza, S., López, I., and Espinoza, M.

Subjects
*RADIATION dosimetry, *HUMAN cytogenetics, *NUCLEOLUS, *LYMPHOCYTES, *GAMMA rays
Abstract

The main objective of the study was the evaluation of the efficacy and homogeneity with which the laboratories of the region carry out biological dosimetry using the two most useful at the present time cytogenetic indicators: the frequency of dicentrics (dicentrics and rings) and that of micronuclei in human lymphocytes. The intercomparison was carried out from codes slides. The gamma radiation doses employed were 0.7,5, 1.5 and 3 Gy. One sample was not irradiated. In the ± 30% of the true dose interval were included 11 out of 15 doses calculated by dicentrics frequency and 9 out of 12 doses calculated by micronuclei frequency. The true doses were in the interval established with 95% confidence limit in 10 out of 12 dose intervals estimated by micronuclei frequency and in 9 out of 15 dose intervals estimated by dicentrics frequency. The laboratory results were similar. [ABSTRACT FROM AUTHOR]

Published
1994

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