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8. Recommendations on ELISpot assay validation by the GCC

Author

Islam, Rafiq, primary, Vance, Jennifer, additional, Poirier, Martin, additional, Zimmer, Jennifer, additional, Khadang, Ardeshir, additional, Williams, Dave, additional, Zemo, Jennifer, additional, Lester, Todd, additional, Fjording, Marianne, additional, Hays, Amanda, additional, Hughes, Nicola, additional, Garofolo, Fabio, additional, Sheldon, Curtis, additional, Guilbaud, Rudolf, additional, Satterwhite, Christina, additional, Colletti, Kelly, additional, Groeber, Elizabeth, additional, Renfrew, Heidi, additional, Yu, Mathilde, additional, Lin, Jenny, additional, Fang, Xinping, additional, Wissel, Mark, additional, Beadnell, Thomas, additional, Lin, John, additional, Shah, Santosh, additional, Garofolo, Wei, additional, Savoie, Natasha, additional, Hayes, Roger, additional, Pirro, John, additional, Kane, Cheikh, additional, Luna, Marsha, additional, Xu, Allan, additional, Cape, Stephanie, additional, O'Dell, Mark, additional, Wheller, Robert, additional, Ritzen, Hanna, additional, Farley, Esme, additional, Kierstead, Lisa, additional, Mylott, William, additional, Tabler, Edward, additional, Yuan, Moucun, additional, Karnik, Shane, additional, Voelker, Troy, additional, DuBey, Ira, additional, Williard, Clark, additional, Dong, Kelly, additional, Shi, Jing, additional, and Yamashita, Jim, additional

Published
2022
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12. Recommendations for the content and management of Certificates of Analysis for reference standards from the GCC for bioanalysis

Author

Bower, Joseph, primary, Zimmer, Jennifer, additional, McCown, Stacie, additional, Tabler, Edward, additional, Karnik, Shane, additional, Kar, Sumit, additional, Xu, Allan, additional, Hays, Amanda, additional, Keyhani, Anahita, additional, Brant, Ashley, additional, Hoffpauir, Brian, additional, Kane, Cheikh, additional, Beaver, Chris, additional, Satterwhite, Christina, additional, Barry, Colin, additional, Desai-Krieger, Daksha, additional, Williams, Dave, additional, Dufield, Dawn, additional, Groeber, Elizabeth, additional, Farley, Esme, additional, Garofolo, Fabio, additional, Renfrew, Heidi, additional, Vance, Jennifer, additional, Lin, Jenny, additional, Dong, Kelly, additional, Sales, Kurt, additional, O'Dell, Mark, additional, Luna, Marsha, additional, Sanghvi, Mitesh, additional, Savoie, Natasha, additional, Bravo, Orlando, additional, Sun, Rachel, additional, Islam, Rafiq, additional, Hayes, Roger, additional, Simchik, Sarah, additional, Cape, Stephanie, additional, Lowes, Steve, additional, Lester, Todd, additional, Garofolo, Wei, additional, and Fang, Xinping, additional

Published
2021
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18. Comparative strategies for stem cell biodistribution in a preclinical study

Author

Wang, Fang, primary, Wang, Zhe, additional, Wang, Fen, additional, Dong, Kelly, additional, Zhang, Jing, additional, Sun, Yun-juan, additional, Liu, Chun-feng, additional, Xing, Mei-jie, additional, Cheng, Xue, additional, Wei, Su, additional, Zheng, Jia-wei, additional, Zhao, Xiong-fei, additional, Wang, Xiao-ming, additional, Fu, Jie, additional, and Song, Hai-feng, additional

Published
2019
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23. Drug Distribution into Peripheral Nerve

Author

Liu, Houfu, primary, Chen, Yan, additional, Huang, Liang, additional, Sun, Xueying, additional, Fu, Tingting, additional, Wu, Shengqian, additional, Zhu, Xiaoyan, additional, Zhen, Wei, additional, Liu, Jihong, additional, Lu, Gang, additional, Cai, Wei, additional, Yang, Ting, additional, Zhang, Wandong, additional, Yu, Xiaohong, additional, Wan, Zehong, additional, Wang, Jianfei, additional, Summerfield, Scott G., additional, Dong, Kelly, additional, and Terstappen, Georg C., additional

Published
2018
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29. Discovery of CNS Penetrant CXCR2 Antagonists for the Potential Treatment of CNS Demyelinating Disorders

Author

Xu, Heng, primary, Lu, Hongfu, additional, Xu, Zhongmiao, additional, Luan, Linbo, additional, Li, Chengyong, additional, Xu, Yan, additional, Dong, Kelly, additional, Zhang, Jinqiang, additional, Li, Xiong, additional, Li, Yvonne, additional, Liu, Gentao, additional, Gong, Sophie, additional, Zhao, Yong-Gang, additional, Liu, Ailian, additional, Zhang, Yueting, additional, Zhang, Wei, additional, Cai, Xin, additional, Xiang, Jia-Ning, additional, Elliott, John D., additional, and Lin, Xichen, additional

Published
2016
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34. Hypoxia in Drosophila Embronic Development.

Author

DONG, KELLY

Subjects
*HYPOXEMIA, *EFFECT of temperature on embryos, *DROSOPHILA melanogaster
Abstract

In organ preservation, the prevalent problem has always been time: time to analyze the organ, match the organ to a recipient, store the organ, transport the organ, and transplant the organ. Due to the quick rate at which tissue deteriorates outside the body, whether that be a result of loss of blood flow or cellular damage from the hypothermic method of storage. The experiment investigates the effect that low oxygen (hypoxia) and low temperature has on the development of embryos as an alternative that may be less detrimental. As demonstrated on the test subject Drosophila melanogaster (fruit flies), both factors can induce a state of suspended animation, which is a condition in which the test subject stops aging during development. Hypoxia can successfully arrest development for up to three times the regular development time, which is around 22 hours at 25°C. At lower temperatures, a greater number of oxygen concentrations are viable and are able to induce suspended animation for a greater period of time. The results of the experiment may suggest that hypoxia is a possible alternative that may extend the time and reduce tissue damage for organ preservation. [ABSTRACT FROM AUTHOR]

Published
2015

36. Antivascular and antitumor evaluation of 2-amino-4-(3-bromo-4,5-dimethoxy-phenyl)-3-cyano-4H-chromenes, a novel series of anticancer agents

Author

Gourdeau, Henriette, primary, Leblond, Lorraine, additional, Hamelin, Bettina, additional, Desputeau, Clemence, additional, Dong, Kelly, additional, Kianicka, Irenej, additional, Custeau, Dominique, additional, Boudreau, Chantal, additional, Geerts, Lilianne, additional, Cai, Sui-Xiong, additional, Drewe, John, additional, Labrecque, Denis, additional, Kasibhatla, Shailaja, additional, and Tseng, Ben, additional

Published
2004
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38. Genetic Changes in Experimental Populations of a Hybrid in the Cryptococcus neoformans Species Complex.

Author

Dong, Kelly, You, Man, and Xu, Jianping

Subjects
CRYPTOCOCCUS neoformans, HETEROZYGOSITY, CHROMOSOME duplication, GENETIC markers, ALLELES, SPECIES, DEMOGRAPHIC change
Abstract

Hybrids between Cryptococcus neoformans and Cryptococcus deneoformans are commonly found in patients and the environment. However, the genetic stability of these hybrids remains largely unknown. Here, we established mutation accumulation lines of a diploid C. neoformans × C. deneoformans laboratory hybrid and analyzed the genotypes at 33 markers distributed across all 14 chromosomes. Our analyses found that under standard culture conditions, heterozygosity at most loci was maintained over 800 mitotic generations, with an estimated 6.44 × 10−5 loss-of-heterozygosity (LoH) event per mitotic division. However, under fluconazole stress, the observed LoH frequency increased by > 50 folds for the two markers on Chromosome 1, all due to the loss of the fluconazole susceptible allele on this chromosome. Flow cytometry analyses showed that after the 40th transfer (120 days), 19 of the 20 lines maintained the original ploidy level (2N), while one line was between 2N and 3N. The combined flow cytometry, genotyping at 33 markers, and quantitative PCR analyses showed the allelic loss was compensated for by amplification of the resistant ERG11 allele in eight of the ten fluconazole-stress lines. Our results suggest that hybrids in C. neoformans species complex are generally stable but that they can undergo rapid adaptation to environmental stresses through LoH and gene duplication. [ABSTRACT FROM AUTHOR]

Published
2020
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