49 results on '"Fattori, M"'
Search Results
2. Spatial Bloch oscillations of a quantum gas in a 'beat-note' superlattice
- Author
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Masi, L., Petrucciani, T., Ferioli, G., Semeghini, G., Modugno, G., Inguscio, M., and Fattori, M.
- Subjects
Condensed Matter - Quantum Gases - Abstract
We report the experimental realization of a new kind of optical lattice for ultra-cold atoms where arbitrarily large separation between the sites can be achieved without renouncing to the stability of ordinary lattices. Two collinear lasers, with slightly different commensurate wavelengths and retroreflected on a mirror, generate a superlattice potential with a periodic "beat-note" profile where the regions with large amplitude modulation provide the effective potential minima for the atoms. To prove the analogy with a standard large spacing optical lattice we study Bloch oscillations of a Bose Einstein condensate with negligible interactions in the presence of a small force. The observed dynamics between sites separated by ten microns for times exceeding one second proves the high stability of the potential. This novel lattice is the ideal candidate for the coherent manipulation of atomic samples at large spatial separations and might find direct application in atom-based technologies like trapped atom interferometers and quantum simulators., Comment: 5 pages, 4 figures
- Published
- 2021
- Full Text
- View/download PDF
3. Sensitivity bounds of a spatial Bloch-oscillations Atom Interferometer
- Author
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Nałȩcz, I., Masi, L., Ferioli, G., Petrucciani, T., Fattori, M., and Chwedeńczuk, J.
- Subjects
Quantum Physics ,Condensed Matter - Quantum Gases - Abstract
We study the ultimate bounds on the sensitivity of a Bloch-oscillation atom interferometer where the external force is estimated from the measurement of the on-site atomic density. For external forces such that the energy difference between lattice sites is smaller than the tunneling energy, the atomic wave-function spreads over many lattice sites, increasing the separation between the occupied modes of the lattice and naturally enhancing the sensitivity of the interferometer. To investigate the applicability of this scheme we estimate the effect of uncontrolled fluctuations of the tunneling energy and the finite resolution of the atom detection. Our analysis shows that a horizontal lattice combined with a weak external force allow for high sensitivities. Therefore, this setup is a promising solution for compact devices or for measurements with high spatial resolution., Comment: 10 pages, 5 figures
- Published
- 2020
4. Dynamical formation of quantum droplets in a $^{39}$K mixture
- Author
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Ferioli, G., Semeghini, G., Terradas-Briansó, S., Masi, L., Fattori, M., and Modugno, M.
- Subjects
Condensed Matter - Quantum Gases ,Physics - Atomic Physics ,Quantum Physics - Abstract
We report on the dynamical formation of self-bound quantum droplets in attractive mixtures of $^{39}$K atoms. Considering the experimental observations of Semeghini et al., Phys. Rev. Lett. 120, 235301 (2018), we perform numerical simulations to understand the relevant processes involved in the formation of a metastable droplet from an out-of-equilibrium mixture. We first analyze the so-called self-evaporation mechanism, where the droplet dissipates energy by releasing atoms, and then we consider the effects of losses due to three-body recombinations and to the balancing of populations in the mixture. We discuss the importance of these three mechanisms in the observed droplet dynamics and their implications for future experiments.
- Published
- 2019
- Full Text
- View/download PDF
5. Self-bound quantum droplets in atomic mixtures
- Author
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Semeghini, G., Ferioli, G., Masi, L., Mazzinghi, C., Wolswijk, L., Minardi, F., Modugno, M., Modugno, G., Inguscio, M., and Fattori, M.
- Subjects
Condensed Matter - Quantum Gases ,Physics - Atomic and Molecular Clusters ,Physics - Atomic Physics ,Quantum Physics - Abstract
Self-bound quantum droplets are a newly discovered phase in the context of ultracold atoms. In this work we report their experimental realization following the original proposal by Petrov [Phys. Rev. Lett. 115, 155302 (2015)], using an attractive bosonic mixture. In this system spherical droplets form due to the balance of competing attractive and repulsive forces, provided by the mean-field energy close to the collapse threshold and the first-order correction due to quantum fluctuations. Thanks to an optical levitating potential with negligible residual confinement we observe self-bound droplets in free space and we characterize the conditions for their formation as well as their equilibrium properties. This work sets the stage for future studies on quantum droplets, from the measurement of their peculiar excitation spectrum, to the exploration of their superfluid nature.
- Published
- 2017
- Full Text
- View/download PDF
6. Crossing Over from Attractive to Repulsive Interactions in a Tunneling Bosonic Josephson Junction
- Author
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Spagnolli, G., Semeghini, G., Masi, L., Ferioli, G., Trenkwalder, A., Coop, S., Landini, M., Pezze', L., Modugno, G., Inguscio, M., Smerzi, A., and Fattori, M.
- Subjects
Quantum Physics ,Condensed Matter - Quantum Gases - Abstract
We explore the interplay between tunneling and interatomic interactions in the dynamics of a bosonic Josephson junction. We tune the scattering length of an atomic $^{39}$K Bose-Einstein condensate confined in a double-well trap to investigate regimes inaccessible to other superconducting or superfluid systems. In the limit of small-amplitude oscillations, we study the transition from Rabi to plasma oscillations by crossing over from attractive to repulsive interatomic interactions. We observe a critical slowing down in the oscillation frequency by increasing the strength of an attractive interaction up to the point of a quantum phase transition. With sufficiently large initial oscillation amplitude and repulsive interactions the system enters the macroscopic quantum self-trapping regime, where we observe coherent undamped oscillations with a self-sustained average imbalance of the relative well population. The exquisite agreement between theory and experiments enables the observation of a broad range of many body coherent dynamical regimes driven by tunable tunneling energy, interactions and external forces, with applications spanning from atomtronics to quantum metrology., Comment: 10 pages, 8 figures, supplemental materials are included
- Published
- 2017
- Full Text
- View/download PDF
7. Observation of Quantum Phase Transitions with Parity-Symmetry Breaking and Hysteresis
- Author
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Trenkwalder, A., Spagnolli, G., Semeghini, G., Coop, S., Landini, M., Castilho, P., Pezzè, L., Modugno, G., Inguscio, M., Smerzi, A., and Fattori, M.
- Subjects
Condensed Matter - Quantum Gases ,Physics - Atomic Physics ,Quantum Physics - Abstract
Symmetry-breaking quantum phase transitions play a key role in several condensed matter, cosmology and nuclear physics theoretical models. Its observation in real systems is often hampered by finite temperatures and limited control of the system parameters. In this work we report for the first time the experimental observation of the full quantum phase diagram across a transition where the spatial parity symmetry is broken. Our system is made of an ultra-cold gas with tunable attractive interactions trapped in a spatially symmetric double-well potential. At a critical value of the interaction strength, we observe a continuous quantum phase transition where the gas spontaneously localizes in one well or the other, thus breaking the underlying symmetry of the system. Furthermore, we show the robustness of the asymmetric state against controlled energy mismatch between the two wells. This is the result of hysteresis associated with an additional discontinuous quantum phase transition that we fully characterize. Our results pave the way to the study of quantum critical phenomena at finite temperature, the investigation of macroscopic quantum tunneling of the order parameter in the hysteretic regime and the production of strongly quantum entangled states at critical points., Comment: 16 pages, 5 figures. A new revised version has been accepted for publication in Nature Physics
- Published
- 2016
- Full Text
- View/download PDF
8. Phase-noise protection in quantum-enhanced differential interferometry
- Author
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Landini, M., Fattori, M., Pezze, L., and Smerzi, A.
- Subjects
Quantum Physics - Abstract
Differential interferometry (DI) with two coupled sensors is a most powerful approach for precision measurements in presence of strong phase noise. However DI has been studied and implemented only with classical resources. Here we generalize the theory of differential interferometry to the case of entangled probe states. We demonstrate that, for perfectly correlated interferometers and in the presence of arbitrary large phase noise, sub-shot noise sensitivities -- up to the Heisenberg limit -- are still possible with a special class of entangled states in the ideal lossless scenario. These states belong to a decoherence free subspace where entanglement is passively protected. Our work pave the way to the full exploitation of entanglement in precision measurements in presence of strong phase noise., Comment: 18 pages, 5 figures, remarks and comments are welcome
- Published
- 2014
- Full Text
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9. Sub-Doppler laser cooling of potassium atoms
- Author
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Landini, M., Roy, S., Carcagni', L., Trypogeorgos, D., Fattori, M., Inguscio, M., and Modugno, G.
- Subjects
Physics - Atomic Physics ,Condensed Matter - Quantum Gases - Abstract
We investigate sub-Doppler laser cooling of bosonic potassium isotopes, whose small hyperfine splitting has so far prevented cooling below the Doppler temperature. We find instead that the combination of a dark optical molasses scheme that naturally arises in this kind of systems and an adiabatic ramping of the laser parameters allows to reach sub-Doppler temperatures for small laser detunings. We demonstrate temperatures as low as 25(3)microK and 47(5)microK in high-density samples of the two isotopes 39K and 41K, respectively. Our findings will find application to other atomic systems., Comment: 7 pages, 9 figures
- Published
- 2011
- Full Text
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10. Delocalization of a disordered bosonic system by repulsive interactions
- Author
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Deissler, B., Zaccanti, M., Roati, G., D'Errico, C., Fattori, M., Modugno, M., Modugno, G., and Inguscio, M.
- Subjects
Condensed Matter - Quantum Gases ,Condensed Matter - Disordered Systems and Neural Networks - Abstract
Clarifying the interplay of interactions and disorder is fundamental to the understanding of many quantum systems, including superfluid helium in porous media, granular and thin-film superconductors, and light propagating in disordered media. One central aspect for bosonic systems is the competition between disorder, which tends to localize particles, and weak repulsive interactions, which instead have a delocalizing effect. Since the required degree of independent control of the disorder and of the interactions is not easily achievable in most available physical systems, a systematic experimental investigation of this competition has so far not been possible. Here we employ an ultracold atomic Bose-Einstein condensate with tunable repulsive interactions in a quasi-periodic lattice potential to study this interplay in detail. We characterize the entire delocalization crossover through the study of the average local shape of the wavefunction, the spatial correlations, and the phase coherence. Three different regimes are identified and compared with theoretical expectations: an exponentially localized Anderson glass, the formation of locally coherent fragments, as well as a coherent, extended state. Our results illuminate the role of weak repulsive interactions on disordered bosonic systems and show that the system and the techniques we employ are promising for further investigations of disordered systems with interactions, also in the strongly correlated regime., Comment: 8 pages, 10 figures
- Published
- 2009
- Full Text
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11. Observation of an Efimov spectrum in an atomic system
- Author
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Zaccanti, M., Deissler, B., D'Errico, C., Fattori, M., Jona-Lasinio, M., Müller, S., Roati, G., Inguscio, M., and Modugno, G.
- Subjects
Condensed Matter - Quantum Gases ,Quantum Physics - Abstract
In 1970 V. Efimov predicted a puzzling quantum-mechanical effect that is still of great interest today. He found that three particles subjected to a resonant pairwise interaction can join into an infinite number of loosely bound states even though each particle pair cannot bind. Interestingly, the properties of these aggregates, such as the peculiar geometric scaling of their energy spectrum, are universal, i.e. independent of the microscopic details of their components. Despite an extensive search in many different physical systems, including atoms, molecules and nuclei, the characteristic spectrum of Efimov trimer states still eludes observation. Here we report on the discovery of two bound trimer states of potassium atoms very close to the Efimov scenario, which we reveal by studying three-particle collisions in an ultracold gas. Our observation provides the first evidence of an Efimov spectrum and allows a direct test of its scaling behaviour, shedding new light onto the physics of few-body systems., Comment: 10 pages, 3 figures, 1 table
- Published
- 2009
- Full Text
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12. Magnetic dipolar interaction in an atomic Bose Einstein condensate interferometer
- Author
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Fattori, M., Roati, G., Deissler, B., D'Errico, C., Zaccanti, M., Jona-Lasinio, M., Santos, L., Inguscio, M., and Modugno, G.
- Subjects
Condensed Matter - Other Condensed Matter - Abstract
We study the role played by the magnetic dipole interaction in an atomic interferometer based on an alkali Bose-Einstein condensate with tunable scattering length. We tune the s-wave interaction to zero using a magnetic Feshbach resonance and measure the decoherence of the interferometer induced by the weak residual interaction between the magnetic dipoles of the atoms. We prove that with a proper choice of the scattering length it is possible to compensate for the dipolar interaction and extend the coherence time of the interferometer. We put in evidence the anisotropic character of the dipolar interaction by working with two different experimental configurations for which the minima of decoherence are achieved for a positive and a negative value of the scattering length, respectively. Our results are supported by a theoretical model we develop. This model indicates that the magnetic dipole interaction should not represent a serious source of decoherence in atom interferometers based on Bose-Einstein condensates., Comment: 4 pages, 3 figures
- Published
- 2008
- Full Text
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13. Anderson localization of a non-interacting Bose-Einstein condensate
- Author
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Roati, G., D'Errico, C., Fallani, L., Fattori, M., Fort, C., Zaccanti, M., Modugno, G., Modugno, M., and Inguscio, M.
- Subjects
Condensed Matter - Disordered Systems and Neural Networks - Abstract
One of the most intriguing phenomena in physics is the localization of waves in disordered media. This phenomenon was originally predicted by Anderson, fifty years ago, in the context of transport of electrons in crystals. Anderson localization is actually a much more general phenomenon, and it has been observed in a large variety of systems, including light waves. However, it has never been observed directly for matter waves. Ultracold atoms open a new scenario for the study of disorder-induced localization, due to high degree of control of most of the system parameters, including interaction. Here we employ for the first time a noninteracting Bose-Einstein condensate to study Anderson localization. The experiment is performed with a onedimensional quasi-periodic lattice, a system which features a crossover between extended and exponentially localized states as in the case of purely random disorder in higher dimensions. Localization is clearly demonstrated by investigating transport properties, spatial and momentum distributions. We characterize the crossover, finding that the critical disorder strength scales with the tunnelling energy of the atoms in the lattice. Since the interaction in the condensate can be controlled at will, this system might be employed to solve open questions on the interplay of disorder and interaction and to explore exotic quantum phases., Comment: 5 pages
- Published
- 2008
- Full Text
- View/download PDF
14. Accurate near-threshold model for ultracold KRb dimers from interisotope Feshbach spectroscopy
- Author
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Simoni, A., Zaccanti, M., D'Errico, C., Fattori, M., Roati, G., Inguscio, M., and Modugno, G.
- Subjects
Condensed Matter - Other Condensed Matter - Abstract
We investigate magnetic Feshbach resonances in two different ultracold K-Rb mixtures. Information on the K(39)-Rb(87) isotopic pair is combined with novel and pre-existing observations of resonance patterns for K(40)-Rb(87). Interisotope resonance spectroscopy improves significantly our near-threshold model for scattering and bound-state calculations. Our analysis determines the number of bound states in singlet/triplet potentials and establishes precisely near threshold parameters for all K-Rb pairs of interest for experiments with both atoms and molecules. In addition, the model verifies the validity of the Born-Oppenheimer approximation at the present level of accuracy., Comment: 9 pages, 7 figures
- Published
- 2008
- Full Text
- View/download PDF
15. Feshbach resonances in ultracold K(39)
- Author
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D'Errico, C., Zaccanti, M., Fattori, M., Roati, G., Inguscio, M., Modugno, G., and Simoni, A.
- Subjects
Physics - Atomic Physics - Abstract
We discover several magnetic Feshbach resonances in collisions of ultracold K(39) atoms, by studying atom losses and molecule formation. Accurate determination of the magnetic-field resonance locations allows us to optimize a quantum collision model for potassium isotopes. We employ the model to predict the magnetic-field dependence of scattering lengths and of near-threshold molecular levels. Our findings will be useful to plan future experiments on ultracold potassium atoms and molecules., Comment: 7 pages, 6 figures
- Published
- 2007
- Full Text
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16. A two-frequency acousto-optic modulator driver to improve the beam pointing stability during intensity ramps
- Author
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Fröhlich, B., Lahaye, T., Kaltenhäuser, B., Kübler, H., Müller, S., Koch, T., Fattori, M., and Pfau, T.
- Subjects
Physics - Optics ,Physics - Instrumentation and Detectors - Abstract
We report on a scheme to improve the pointing stability of the first order beam diffracted by an acousto-optic modulator (AOM). Due to thermal effects inside the crystal, the angular position of the beam can change by as much as 1 mrad when the radio-frequency power in the AOM is reduced to decrease the first order beam intensity. This is done for example to perform forced evaporative cooling in ultracold atom experiments using far-off-resonant optical traps. We solve this problem by driving the AOM with two radio-frequencies $f_1$ and $f_2$. The power of $f_2$ is adjusted relative to the power of $f_1$ to keep the total power constant. Using this, the beam displacement is decreased by a factor of twenty. The method is simple to implement in existing experimental setups, without any modification of the optics., Comment: Submitted to Review of Scientific Instruments, 4 pages
- Published
- 2007
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- View/download PDF
17. Spinor condensates with a laser-induced quadratic Zeeman effect
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Santos, L., Fattori, M., Stuhler, J., and Pfau, T.
- Subjects
Condensed Matter - Other Condensed Matter - Abstract
We show that an effective quadratic Zeeman effect can be generated in $^{52}$Cr by proper laser configurations, and in particular by the dipole trap itself. The induced quadratic Zeeman effect leads to a rich ground-state phase diagram, can be used to induce topological defects by controllably quenching across transitions between phases of different symmetries, allows for the observability of the Einstein-de Haas effect for relatively large magnetic fields, and may be employed to create $S=1/2$ systems with spinor dynamics. Similar ideas could be explored in other atomic species opening an exciting new control tool in spinor systems., Comment: 4 pages, 2 eps figures
- Published
- 2006
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18. Damagnetization cooling of a gas
- Author
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Fattori, M., Koch, T., Goetz, S., Griesmaier, A., Hensler, S., Stuhler, J., and Pfau, T.
- Subjects
Condensed Matter - Other Condensed Matter - Abstract
We demonstrate demagnetization cooling of a gas of ultracold $^{52}$Cr atoms. Demagnetization is driven by inelastic dipolar collisions which couple the motional degrees of freedom to the spin degree. By that kinetic energy is converted into magnetic work with a consequent temperature reduction of the gas. Optical pumping is used to magnetize the system and drive continuous demagnetization cooling. Applying this technique, we can increase the phase space density of our sample by one order of magnitude, with nearly no atom loss. This method can be in principle extended to every dipolar system and could be used to achieve quantum degeneracy via optical means., Comment: 10 pages, 5 figures
- Published
- 2006
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19. Atom interferometry gravity-gradiometer for the determination of the Newtonian gravitational constant G
- Author
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Bertoldi, A., Lamporesi, G., Cacciapuoti, L., de Angelis, M., Fattori, M., Petelski, T., Peters, A., prevedelli, M., Stuhler, J., and Tino, G. M.
- Subjects
Physics - Atomic Physics ,Physics - General Physics - Abstract
We developed a gravity-gradiometer based on atom interferometry for the determination of the Newtonian gravitational constant \textit{G}. The apparatus, combining a Rb fountain, Raman interferometry and a juggling scheme for fast launch of two atomic clouds, was specifically designed to reduce possible systematic effects. We present instrument performances and show that the sensor is able to detect the gravitational field induced by source masses. A discussion of projected accuracy for \textit{G} measurement using this new scheme shows that the results of the experiment will be significant to discriminate between previous inconsistent values., Comment: 9 pages,9 figures, Submitted
- Published
- 2006
- Full Text
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20. Expansion dynamics of a dipolar Bose-Einstein condensate
- Author
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Giovanazzi, S., Pedri, P., Santos, L., Griesmaier, A., Fattori, M., Koch, T., Stuhler, J., and Pfau, T.
- Subjects
Condensed Matter - Other Condensed Matter - Abstract
Our recent measurements on the expansion of a chromium dipolar condensate after release from an optical trapping potential are in good agreement with an exact solution of the hydrodynamic equations for dipolar Bose gases. We report here the theoretical method used to interpret the measurement data as well as more details of the experiment and its analysis. The theory reported here is a tool for the investigation of different dynamical situations in time-dependent harmonic traps., Comment: 12 pages. Submitted to PRA
- Published
- 2006
- Full Text
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21. Observation of dipole-dipole interaction in a degenerate quantum gas
- Author
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Stuhler, J., Griesmaier, A., Koch, T., Fattori, M., Pfau, T., Giovanazzi, S., Pedri, P., and Santos, L.
- Subjects
Condensed Matter - Other Condensed Matter - Abstract
We have investigated the expansion of a Bose-Einstein condensate (BEC) of strongly magnetic chromium atoms. The long-range and anisotropic magnetic dipole-dipole interaction leads to an anisotropic deformation of the expanding Cr-BEC which depends on the orientation of the atomic dipole moments. Our measurements are consistent with the theory of dipolar quantum gases and show that a Cr-BEC is an excellent model system to study dipolar interactions in such gases., Comment: 4 pages, 2 figures
- Published
- 2005
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22. Long‐Range Optical Wireless Communication System Based on a Large‐Area, Q‐Dots Fluorescent Antenna
- Author
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Umair, M, Seminara, M, Meucci, M, Fattori, M, Bruni, F, Brovelli, S, Meinardi, F, Catani, J, Umair, Muhammad Ali, Seminara, Marco, Meucci, Marco, Fattori, Marco, Bruni, Francesco, Brovelli, Sergio, Meinardi, Francesco, Catani, Jacopo, Umair, M, Seminara, M, Meucci, M, Fattori, M, Bruni, F, Brovelli, S, Meinardi, F, Catani, J, Umair, Muhammad Ali, Seminara, Marco, Meucci, Marco, Fattori, Marco, Bruni, Francesco, Brovelli, Sergio, Meinardi, Francesco, and Catani, Jacopo
- Abstract
Fluorescent concentrators (FCs) have been recently proposed as optical condensers for visible light communications (VLC) and optical wireless communication (OWC) receivers, with advantages over conventional optical stages in terms of optical gain and field of view (FoV). However, the use of FC-based receivers in real-world scenarios is hampered by the need for large resilience of the involved chromophores to sunlight exposure, and availability of large-area FC substrates with suitable optochemical properties. This paper presents an innovative OWC system, based on a high-power blue LED and a large-area FC-based on CuInS2 quantum dots (Q-Dots) as receiving optical stage. A thorough characterization of the FC material in terms of conversion efficiency, temporal response, and FoV is provided, along with a full set of outdoor measurements. The combination of the Q-Dots fluorophores chemico-physical properties with the strong DC rejection granted by the design receiver stage allows error-free VLC link distances up to 60 m and baud rates up to 1 Mb/s. This work represents the first demonstration of long-range VLC links under strong solar irradiance using large-area FC antennas and paves the way to deployment of long-range free-space optical links with minimal susceptibility to misalignments and pointing instabilities between receiver and transmitter.
- Published
- 2023
23. The prohibition of torture: legal certainty and interpretation of peremptory norms in international law
- Author
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Fattori, M., Fattori, M., Fattori, M., and Fattori, M.
- Published
- 2019
24. Sensitivity bounds of a spatial Bloch-oscillations Atom Interferometer
- Author
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Na����cz, I., Masi, L., Ferioli, G., Petrucciani, T., Fattori, M., and Chwede��czuk, J.
- Subjects
Quantum Physics ,Quantum Gases (cond-mat.quant-gas) ,FOS: Physical sciences ,Quantum Physics (quant-ph) ,Condensed Matter - Quantum Gases - Abstract
We study the ultimate bounds on the sensitivity of a Bloch-oscillation atom interferometer where the external force is estimated from the measurement of the on-site atomic density. For external forces such that the energy difference between lattice sites is smaller than the tunneling energy, the atomic wave-function spreads over many lattice sites, increasing the separation between the occupied modes of the lattice and naturally enhancing the sensitivity of the interferometer. To investigate the applicability of this scheme we estimate the effect of uncontrolled fluctuations of the tunneling energy and the finite resolution of the atom detection. Our analysis shows that a horizontal lattice combined with a weak external force allow for high sensitivities. Therefore, this setup is a promising solution for compact devices or for measurements with high spatial resolution., 10 pages, 5 figures
- Published
- 2020
25. Polyamine depletion inhibits etoposide-induced NF-κB activation in transformed mouse fibroblasts
- Author
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Tantini, B., Pignatti, C., Fattori, M., Fiumana, E., Facchini, A., Stefanelli, C., Caldarera, C. M., Pegg, A. E., and Flamigni, F.
- Published
- 2004
- Full Text
- View/download PDF
26. Nota linguistico-esegetica ad Aen. 2, 121
- Author
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Fattori, M and Fattori, M
- Subjects
Virgil ,Aeneid 2.121 ,paro ,intransitivization ,anticausativization - Abstract
Linguistic-exegetical note on Aen. 2, 121. This paper aims to offer a linguistic explanation for the unexpected use of the verb paro in Aen. 2.121. Drawing on parallel passages in Homer, and comparing other cases of intransitive constructions of usually transitive verbs in Latin, the author argues that paro assumes here a reflexive meaning while maintaining an active intransitive construction, which is technically called an 'anticausative' value.
- Published
- 2019
27. Dynamical formation of quantum droplets in a K39 mixture
- Author
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Ferioli, G., primary, Semeghini, G., additional, Terradas-Briansó, S., additional, Masi, L., additional, Fattori, M., additional, and Modugno, M., additional
- Published
- 2020
- Full Text
- View/download PDF
28. The identification of Bradyrhizobium japonicum strains isolated from Italian soils
- Author
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Kay, H.E., Coutinho, H.L.C., Fattori, M., Manfio, G.P., Goodacre, R., Nuti, M.P., Basaglia, M., and Beringer, J.E.
- Subjects
Rhizobium -- Research ,Polymerase chain reaction -- Analysis ,Biological sciences - Abstract
Polymerase chain reaction analysis with arbitrary primers and pyrolysis mass spectrometry (PYMS) help isolate and characterize Bradyrhizobium japonicum strains from Northern Italian fields. The two techniques help distinguish whether the strains are inoculate derivatives and indicate the presence of a CB1809 strain as an inoculant. PYMS data also reveal that two specific derivative populations arise from the inoculant, one exhibiting a modified phenotype and the other resembling the parent strain.
- Published
- 1994
29. Quantum phase transitions with parity-symmetry breaking and hysteresis
- Author
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Trenkwalder, A., Spagnolli, G., Semeghini, Giulia, Coop, S., Landini, M., Castilho, P., Pezzè, L., Modugno, Giovanni, Inguscio, Massimo, Smerzi, M. Fattori, A. Smerzi, and Fattori, M.
- Subjects
Transizioni di fase quantistiche ,Condensed Matter::Quantum Gases ,Quantum phase transition ,Physics ,Condensed matter physics ,Condensed Matter::Other ,Cavity quantum electrodynamics ,General Physics and Astronomy ,Quantum simulator ,Parity (physics) ,Quantum phases ,Condensed Matter::Mesoscopic Systems and Quantum Hall Effect ,01 natural sciences ,Article ,010305 fluids & plasmas ,FÍSICA ATÔMICA ,Quantum mechanics ,Quantum critical point ,0103 physical sciences ,Principal quantum number ,Bose-Einstein condensate ,double-well ,gas ,Physics::Atomic Physics ,Symmetry breaking ,010306 general physics - Abstract
Symmetry-breaking quantum phase transitions play a key role in several condensed matter, cosmology and nuclear physics theoretical models(1-3). Its observation in real systems is often hampered by finite temperatures and limited control of the system parameters. In this work we report, for the first time, the experimental observation of the full quantum phase diagram across a transition where the spatial parity symmetry is broken. Our system consists of an ultracold gas with tunable attractive interactions trapped in a spatially symmetric double-well potential. At a critical value of the interaction strength, we observe a continuous quantum phase transition where the gas spontaneously localizes in one well or the other, thus breaking the underlying symmetry of the system. Furthermore, we show the robustness of the asymmetric state against controlled energy mismatch between the two wells. This is the result of hysteresis associated with an additional discontinuous quantum phase transition that we fully characterize. Our results pave the way to the study of quantum critical phenomena at finite temperature(4), the investigation of macroscopic quantum tunnelling of the order parameter in the hysteretic regime and the production of strongly quantum entangled states at critical points(5).
- Published
- 2016
- Full Text
- View/download PDF
30. Carbon dioxide extraction of canola seed: Oil solubility and effect of seed treatment
- Author
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Fattori, M., Bulley, N. R., and Meisen, A.
- Published
- 1988
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31. Modelling of supercritical carbon dioxide extraction of canola oilseed in fixed beds
- Author
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Lee, A. K. K., Bulley, N. R., Fattori, M., and Meisen, A.
- Published
- 1986
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32. Supercritical fluid extraction of vegetable oil seeds
- Author
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Bulley, N. R., Fattori, M., Meisen, A., and Moyls, L.
- Published
- 1984
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33. Phase-noise protection in quantum-enhanced differential interferometry
- Author
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Landini, M, primary, Fattori, M, additional, Pezzè, L, additional, and Smerzi, A, additional
- Published
- 2014
- Full Text
- View/download PDF
34. Polyamines, nitric oxide and cyclic GMP mediate stimulation of DNA synthesis by tumor necrosis factor and lipopolysaccharide in chick embryo cardiomyocytes
- Author
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Giordano, E., primary, Tantini, B., additional, Flamigni, F., additional, Pignatti, C., additional, Stefanelli, C., additional, Fattori, M., additional, Facchini, A., additional, Clo', C., additional, Caldarera, C.M., additional, and Guarnieri, C., additional
- Published
- 2000
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35. Soldadura dos ferros fundidos cinzentos, nodulares e bainíticos
- Author
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Amaral, Henrique M. Fattori M.
- Subjects
Materiais e processos de fabrico ,Ferro fundido ,Soldadura ,Engenharia mecânica - Abstract
Dissertação apresentada para obtenção do grau de Mestre em Materiais e Processos de Fabrico, na Faculdade de Engenharia da Universidade do Porto
- Published
- 1989
36. L'evento conciliare nell'analisi degli osservatori ortodossi russi e del Consiglio per gli affari della chiesa ortodossa russa. L'esperienza della prima sessione
- Author
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ROCCUCCI, ADRIANO, FATTORI M T, MELLONI A, and Roccucci, Adriano
- Published
- 1997
37. CLARE ET DISTINCTE NELLE MEDITATIONS/MEDITATIONES DI DESCARTES
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BELGIOIOSO, Giulia, Fattori, M. (ed.), and Belgioioso, Giulia
- Published
- 1997
38. Lessico di Giordano Bruno
- Author
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CILIBERTO, Michele, Ciliberto, Michele, Aa. Vv., and FATTORI M, BIANCHI M. L
- Published
- 1979
39. Brain-Computer Interfaces Using Flexible Electronics: An a-IGZO Front-End for Active ECoG Electrodes.
- Author
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van Oosterhout K, Chilundo A, Branco MP, Aarnoutse EJ, Timmermans M, Fattori M, Ramsey NF, and Cantatore E
- Abstract
Brain-computer interfaces (BCIs) are evolving toward higher electrode count and fully implantable solutions, which require extremely low power densities (<15mW cm
-2 ). To achieve this target, and allow for a large and scalable number of channels, flexible electronics can be used as a multiplexing interface. This work introduces an active analog front-end fabricated with amorphous Indium-Gallium-Zinx-Oxide (a-IGZO) Thin-Film Transistors (TFTs) on foil capable of active matrix multiplexing. The circuit achieves only 70nV per sqrt(Hz) input referred noise, consuming 46µW, or 3.5mW cm-2 . It demonstrates for the first time in literature a flexible front-end with a noise efficiency factor comparable with Silicon solutions (NEF = 9.8), which is more than 10X lower compared to previously reported flexible front-ends. These results have been achieved using a modified bootstrap-load amplifier. The front end is tested by playing through it recordings obtained from a conventional BCI system. A gesture classification based on the flexible front-end outputs achieves 94% accuracy. Using a flexible active front end can improve the state-of-the-art in high channel count BCI systems by lowering the multiplexer noise and enabling larger areas of the brain to be monitored while reducing power density. Therefore, this work enables a new generation of high channel-count active BCI electrode grids., (© 2024 The Author(s). Advanced Science published by Wiley‐VCH GmbH.)- Published
- 2024
- Full Text
- View/download PDF
40. Hardware implementation of backpropagation using progressive gradient descent for in situ training of multilayer neural networks.
- Author
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van Doremaele ERW, Stevens T, Ringeling S, Spolaor S, Fattori M, and van de Burgt Y
- Abstract
Neural network training can be slow and energy-expensive due to the frequent transfer of weight data between digital memory and processing units. Neuromorphic systems can accelerate neural networks by performing multiply-accumulate operations in parallel using nonvolatile analog memory. However, executing the widely used backpropagation training algorithm in multilayer neural networks requires information-and therefore storage-of the partial derivatives of the weight values preventing suitable and scalable implementation in hardware. Here, we propose a hardware implementation of the backpropagation algorithm that progressively updates each layer using in situ stochastic gradient descent, avoiding this storage requirement. We experimentally demonstrate the in situ error calculation and the proposed progressive backpropagation method in a multilayer hardware-implemented neural network. We confirm identical learning characteristics and classification performance compared to conventional backpropagation in software. We show that our approach can be scaled to large and deep neural networks, enabling highly efficient training of advanced artificial intelligence computing systems.
- Published
- 2024
- Full Text
- View/download PDF
41. Vitality surveillance at distance using thin-film tandem-like narrowband near-infrared photodiodes with light-enhanced responsivity.
- Author
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Ollearo R, Ma X, Akkerman HB, Fattori M, Dyson MJ, van Breemen AJJM, Meskers SCJ, Dijkstra W, Janssen RAJ, and Gelinck GH
- Abstract
Remote measurement of vital sign parameters like heartbeat and respiration rate represents a compelling challenge in monitoring an individual's health in a noninvasive way. This could be achieved by large field-of-view, easy-to-integrate unobtrusive sensors, such as large-area thin-film photodiodes. At long distances, however, discriminating weak light signals from background disturbance demands superior near-infrared (NIR) sensitivity and optical noise tolerance. Here, we report an inherently narrowband solution-processed, thin-film photodiode with ultrahigh and controllable NIR responsivity based on a tandem-like perovskite-organic architecture. The device has low dark currents (<10
-6 mA cm-2 ), linear dynamic range >150 dB, and operational stability over time (>8 hours). With a narrowband quantum efficiency that can exceed 200% at 850 nm and intrinsic filtering of other wavelengths to limit optical noise, the device exhibits higher tolerance to background light than optically filtered silicon-based sensors. We demonstrate its potential in remote monitoring by measuring the heart rate and respiration rate from distances up to 130 cm in reflection.- Published
- 2023
- Full Text
- View/download PDF
42. Ultralow dark current in near-infrared perovskite photodiodes by reducing charge injection and interfacial charge generation.
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Ollearo R, Wang J, Dyson MJ, Weijtens CHL, Fattori M, van Gorkom BT, van Breemen AJJM, Meskers SCJ, Janssen RAJ, and Gelinck GH
- Abstract
Metal halide perovskite photodiodes (PPDs) offer high responsivity and broad spectral sensitivity, making them attractive for low-cost visible and near-infrared sensing. A significant challenge in achieving high detectivity in PPDs is lowering the dark current density (J
D ) and noise current (in ). This is commonly accomplished using charge-blocking layers to reduce charge injection. By analyzing the temperature dependence of JD for lead-tin based PPDs with different bandgaps and electron-blocking layers (EBL), we demonstrate that while EBLs eliminate electron injection, they facilitate undesired thermal charge generation at the EBL-perovskite interface. The interfacial energy offset between the EBL and the perovskite determines the magnitude and activation energy of JD . By increasing this offset we realized a PPD with ultralow JD and in of 5 × 10-8 mA cm-2 and 2 × 10-14 A Hz-1/2 , respectively, and wavelength sensitivity up to 1050 nm, establishing a new design principle to maximize detectivity in perovskite photodiodes., (© 2021. The Author(s).)- Published
- 2021
- Full Text
- View/download PDF
43. Clinical assessment instruments validated for nursing practice in the Italian context: a systematic review of the literature.
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Palese A, Tameni A, Ambrosi E, Albanese S, Barausse M, Benazzi B, De Togni S, Doro R, Eccher C, Fattori M, Franchini P, Girlanda M, Gobbetti D, Guarino L, Lazzeri R, Moreale R, Ricci N, Venturini M, Villa G, Zonzini E, and Saiani L
- Subjects
- Humans, Italy, Reproducibility of Results, Nursing standards, Professional Practice standards
- Abstract
Aims: With the aim to identify the instruments validated for Italian nursing practice, a systematic review of the literature was undertaken., Results: A total of 101 instruments emerged. The majority (89; 88.1%) were developed in other countries; the remaining (14; 13.9%) were developed and validated in the Italian context. The instruments were developed to measure patient's problems (63/101; 62.4%), outcomes (27/101; 26.7%), risks (4/101; 4%) and others issues (7/101; 6.9%). The majority of participants involved in the validation processes were younger adults (49; 48.5%), older adults (40; 39.5%), children (4; 4%), adolescents (3; 3%), and children/adolescents (1; 1%). The instruments were structured primarily in the form of questionnaires (61; 60.4%), as a grid for direct observation (27; 26.7%) or in other forms (12; 11.9%). Among the 101 instruments emerged, there were 1 to 7 validation measures documented with on average 3.2 (95% CI 2.86-3.54) for each instrument., Conclusions: Developing validation studies giving priority to those instruments widely adopted in the clinical nursing practice is recommended.
- Published
- 2014
- Full Text
- View/download PDF
44. Strong dipolar effects in a quantum ferrofluid.
- Author
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Lahaye T, Koch T, Fröhlich B, Fattori M, Metz J, Griesmaier A, Giovanazzi S, and Pfau T
- Abstract
Symmetry-breaking interactions have a crucial role in many areas of physics, ranging from classical ferrofluids to superfluid (3)He and d-wave superconductivity. For superfluid quantum gases, a variety of new physical phenomena arising from the symmetry-breaking interaction between electric or magnetic dipoles are expected. Novel quantum phases in optical lattices, such as chequerboard or supersolid phases, are predicted for dipolar bosons. Dipolar interactions can also enrich considerably the physics of quantum gases with internal degrees of freedom. Arrays of dipolar particles could be used for efficient quantum information processing. Here we report the realization of a chromium Bose-Einstein condensate with strong dipolar interactions. By using a Feshbach resonance, we reduce the usual isotropic contact interaction, such that the anisotropic magnetic dipole-dipole interaction between 52Cr atoms becomes comparable in strength. This induces a change of the aspect ratio of the atom cloud; for strong dipolar interactions, the inversion of ellipticity during expansion (the usual 'smoking gun' evidence for a Bose-Einstein condensate) can be suppressed. These effects are accounted for by taking into account the dipolar interaction in the superfluid hydrodynamic equations governing the dynamics of the gas, in the same way as classical ferrofluids can be described by including dipolar terms in the classical hydrodynamic equations. Our results are a first step in the exploration of the unique properties of quantum ferrofluids.
- Published
- 2007
- Full Text
- View/download PDF
45. IRAK-M is involved in the pathogenesis of early-onset persistent asthma.
- Author
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Balaci L, Spada MC, Olla N, Sole G, Loddo L, Anedda F, Naitza S, Zuncheddu MA, Maschio A, Altea D, Uda M, Pilia S, Sanna S, Masala M, Crisponi L, Fattori M, Devoto M, Doratiotto S, Rassu S, Mereu S, Giua E, Cadeddu NG, Atzeni R, Pelosi U, Corrias A, Perra R, Torrazza PL, Pirina P, Ginesu F, Marcias S, Schintu MG, Del Giacco GS, Manconi PE, Malerba G, Bisognin A, Trabetti E, Boner A, Pescollderungg L, Pignatti PF, Schlessinger D, Cao A, and Pilia G
- Subjects
- Adolescent, Age of Onset, Alleles, Alternative Splicing, Amino Acid Substitution, Asthma diagnosis, Asthma pathology, Case-Control Studies, Chromosome Mapping, Chromosomes, Human, Pair 12, Cohort Studies, Female, Founder Effect, Gene Frequency, Genetic Linkage, Genetic Markers, Genetic Predisposition to Disease, Haplotypes, Humans, Immunohistochemistry, Interleukin-1 Receptor-Associated Kinases metabolism, Italy epidemiology, Linkage Disequilibrium, Lod Score, Lung metabolism, Lung surgery, Male, Microsatellite Repeats, Mutation, Missense, Polymorphism, Single Nucleotide, Siblings, Asthma epidemiology, Asthma etiology, Asthma genetics, Interleukin-1 Receptor-Associated Kinases genetics
- Abstract
Asthma is a multifactorial disease influenced by genetic and environmental factors. In the past decade, several loci and >100 genes have been found to be associated with the disease in at least one population. Among these loci, region 12q13-24 has been implicated in asthma etiology in multiple populations, suggesting that it harbors one or more asthma susceptibility genes. We performed linkage and association analyses by transmission/disequilibrium test and case-control analysis in the candidate region 12q13-24, using the Sardinian founder population, in which limited heterogeneity of pathogenetic alleles for monogenic and complex disorders as well as of environmental conditions should facilitate the study of multifactorial traits. We analyzed our cohort, using a cutoff age of 13 years at asthma onset, and detected significant linkage to a portion of 12q13-24. We identified IRAK-M as the gene contributing to the linkage and showed that it is associated with early-onset persistent asthma. We defined protective and predisposing SNP haplotypes and replicated associations in an outbred Italian population. Sequence analysis in patients found mutations, including inactivating lesions, in the IRAK-M coding region. Immunohistochemistry of lung biopsies showed that IRAK-M is highly expressed in epithelial cells. We report that IRAK-M is involved in the pathogenesis of early-onset persistent asthma. IRAK-M, a negative regulator of the Toll-like receptor/IL-1R pathways, is a master regulator of NF- kappa B and inflammation. Our data suggest a mechanistic link between hyperactivation of the innate immune system and chronic airway inflammation and indicate IRAK-M as a potential target for therapeutic intervention against asthma.
- Published
- 2007
- Full Text
- View/download PDF
46. Caspase activation in etoposide-treated fibroblasts is correlated to ERK phosphorylation and both events are blocked by polyamine depletion.
- Author
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Stefanelli C, Tantini B, Fattori M, Stanic' I, Pignatti C, Clo C, Guarnieri C, Caldarera CM, Mackintosh CA, Pegg AE, and Flamigni F
- Subjects
- Animals, Apoptosis drug effects, Apoptosis physiology, Caspase Inhibitors, Caspases drug effects, Coumarins pharmacology, Eflornithine pharmacology, Enzyme Activation drug effects, Enzyme Inhibitors pharmacology, Fibroblasts drug effects, Fibroblasts pathology, Mice, Mitogen-Activated Protein Kinase 1 drug effects, Mitogen-Activated Protein Kinase 1 metabolism, Mitogen-Activated Protein Kinase 3, Mitogen-Activated Protein Kinases drug effects, Oligopeptides pharmacology, Ornithine Decarboxylase Inhibitors, Phosphorylation, Putrescine pharmacology, Signal Transduction, Caspases metabolism, Etoposide pharmacology, Fibroblasts metabolism, Mitogen-Activated Protein Kinases metabolism, Polyamines metabolism
- Abstract
Activation of the extracellular signal-regulated kinases (ERKs) 1 and 2 is correlated to cell survival, but in some cases ERKs can act in signal transduction pathways leading to apoptosis. Treatment of mouse fibroblasts with 20 microM etoposide elicited a sustained phosphorylation of ERK 1/2, that increased until 24 h from the treatment in parallel with caspase activity. The inhibitor of ERK activation PD98059 abolished caspase activation, but caspase inhibition did not reduce ERK 1/2 phosphorylation, suggesting that ERK activation is placed upstream of caspases. Both ERK and caspase activation were blocked in cells depleted of polyamines by the ornithine decarboxylase inhibitor alpha-difluoromethylornithine (DFMO). In etoposide-treated cells, DFMO also abolished phosphorylation of c-Jun NH(2)-terminal kinases triggered by the drug. Polyamine replenishment with exogenous putrescine restored the ability of the cells to undergo caspase activation and ERK 1/2 phosphorylation in response to etoposide. Ornithine decarboxylase activity decreased after etoposide, indicating that DFMO exerts its effect by depleting cellular polyamines before induction of apoptosis. These results reveal a role for polyamines in the transduction of the death signal triggered by etoposide.
- Published
- 2002
- Full Text
- View/download PDF
47. NF-kappaB and ERK cooperate to stimulate DNA synthesis by inducing ornithine decarboxylase and nitric oxide synthase in cardiomyocytes treated with TNF and LPS.
- Author
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Tantini B, Pignatti C, Fattori M, Flamigni F, Stefanelli C, Giordano E, Menegazzi M, Clô C, and Caldarera CM
- Subjects
- Animals, Cell Division, Chick Embryo, Enzyme Induction, Lipopolysaccharides pharmacology, Mitogens pharmacology, Myocardium metabolism, Signal Transduction, Tumor Necrosis Factor-alpha pharmacology, Mitogen-Activated Protein Kinases metabolism, Myocardium cytology, NF-kappa B metabolism, Nitric Oxide Synthase biosynthesis, Ornithine Decarboxylase biosynthesis
- Abstract
We previously reported that tumor necrosis factor-alpha (TNF) and lipopolysaccharide (LPS) stimulate DNA synthesis in chick embryo cardiomyocytes (CM) via nitric oxide and polyamine biosynthesis. Here we show an involvement of nuclear factor-kappaB (NF-kappaB) in the induction of nitric oxide synthase (NOS) and ornithine decarboxylase (ODC), the key enzyme in polyamine biosynthesis. In addition NF-kappaB activation appears to favor survival of CM by reducing caspase activation. TNF and LPS also stimulate phosphorylation of extracellular signal-regulated kinase (ERK), which is required for the changes in ODC and caspase activity, but not for NOS induction or NF-kappaB activation. In conclusion, these results indicate that NF-kappaB, in cooperation with ERK, plays a pivotal role in the growth stimulating effects of TNF and LPS, leading to the induction of both ODC and NOS and to the reduction of caspase activity.
- Published
- 2002
- Full Text
- View/download PDF
48. Effect of polyamine depletion on caspase activation: a study with spermine synthase-deficient cells.
- Author
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Stefanelli C, Pignatti C, Tantini B, Fattori M, Stanic I, Mackintosh CA, Flamigni F, Guarnieri C, Caldarera CM, and Pegg AE
- Subjects
- Animals, Blotting, Western, Cells, Cultured, Cycloheximide pharmacology, Eflornithine pharmacology, Enzyme Activation, Fibroblasts drug effects, Fibroblasts enzymology, Fibroblasts metabolism, Male, Mice, Mice, Mutant Strains, Protein Synthesis Inhibitors pharmacology, Spermine Synthase genetics, Caspases metabolism, Polyamines metabolism, Spermine Synthase metabolism
- Abstract
Activation of the caspase proteases represents a central point in apoptosis. The requirement for spermine for the processes leading to caspase activation has been studied in transformed embryonic fibroblasts obtained from gyro (Gy) mutant male mice. These cells lack spermine synthase activity and thus provide a valuable model to study the role of spermine in cell processes. Gy fibroblasts do not contain spermine and have a higher spermidine content. However, when compared with fibroblasts obtained from normal male littermates (N cells), Gy fibroblasts were observed to grow normally. The lack of spermine did not affect the expression of Bcl-2, and caspases 3 and 9 were activated by etoposide in both N and Gy cells, indicating that spermine is dispensable for caspase activation. Spermine deficiency did not significantly influence caspase activity in cells treated with etoposide, cycloheximide or staurosporine, but sensitized the cells to UV irradiation, which triggered significantly higher caspase activity in Gy cells compared with N cells. alpha-Difluoromethylornithine (DFMO), an inhibitor of polyamine synthesis that is able to deplete cells of putrescine and spermidine, but usually does not influence spermine content, was able to produce a more complete polyamine depletion in Gy cells. This depletion, which included spermine deficiency, dramatically increased caspase activation and cell death in Gy fibroblasts exposed to UV irradiation. On the other hand, in either N or Gy cells, DFMO treatment did not influence caspase activity triggered by staurosporine, but inhibited it when the inducers were cycloheximide or etoposide. In Gy cells depleted of polyamines by DFMO, polyamine replenishment with either spermidine or spermine was sufficient to restore caspase activity induced by etoposide, indicating that, in this model, polyamines have an interchangeable role in supporting caspase activation. Therefore, spermine is not required for such activation, and the effect and specificity of polyamine depletion on caspase activity may be very different, depending on the role of polyamines in the specific death pathways engaged by different stimuli. Some inducers of apoptosis, for example etoposide, absolutely require polyamines for caspase activation, yet the lack of polyamines, particularly spermine, strongly increases caspase activation when induced by UV irradiation.
- Published
- 2001
- Full Text
- View/download PDF
49. Polyamines, NO and cGMP mediate stimulation of DNA synthesis by tumor necrosis factor and lipopolysaccharide in chick embryo cardiomyocytes.
- Author
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Tantini B, Flamigni F, Pignatti C, Stefanelli C, Fattori M, Facchini A, Giordano E, Clô C, and Caldarera CM
- Subjects
- Alkaloids pharmacology, Aminoquinolines pharmacology, Animals, Cells, Cultured, Chick Embryo, Eflornithine pharmacology, Enzyme Activation, Enzyme Inhibitors pharmacology, Guanylate Cyclase antagonists & inhibitors, Guanylate Cyclase metabolism, Lipopolysaccharides pharmacology, Methylene Blue pharmacology, Nitric Oxide Synthase antagonists & inhibitors, Nitric Oxide Synthase metabolism, Ornithine Decarboxylase metabolism, Ornithine Decarboxylase Inhibitors, Protein Kinase Inhibitors, Stimulation, Chemical, Tumor Necrosis Factor-alpha pharmacology, omega-N-Methylarginine pharmacology, Carbazoles, Cyclic GMP metabolism, DNA biosynthesis, Indoles, Myocardium metabolism, Nitric Oxide metabolism, Polyamines metabolism
- Abstract
Objective: We have recently shown that tumor necrosis factor-alpha (TNFalpha) and lipopolysaccharide (LPS) stimulate DNA synthesis in chick embryo cardiomyocytes (CMs). The aim of the present research was to investigate the pathways involved in this mitogenic response., Methods: CMs were isolated from 10-day-old chick embryos and grown to confluence. After 20 h of serum starvation the cells were treated with TNFalpha and LPS, and/or specific agonists and antagonists to manipulate the levels of polyamines, NO, cGMP and their biosynthetic enzymes ornithine decarboxylase (ODC), nitric oxide synthase (NOS) and soluble guanylate cyclase (sGC). ODC, NOS, sGC activities and cGMP contents were determined by radiochemical procedures. DNA synthesis was determined by incorporation of [3H]-thymidine., Results: Treatment of CMs with TNFalpha and LPS increased cell number and [3H]-thymidine incorporation. Addition of TNFalpha and LPS provoked an induction of ODC, with consequent polyamine accumulation, and a more delayed enhancement of NOS activity, which appeared to be independent of the activation of the ODC-polyamine system. TNFalpha and LPS treatment also enhanced cGMP level in CMs and both polyamine and NO biosyntheses appeared to be required. Experiments with specific inhibitors of ODC and NOS, as well as with inhibitors of sGC and cGMP-dependent protein kinase (PKG), showed that polyamine-, NO- and cGMP-dependent pathways are required for the mitogenic action of TNFalpha and LPS. Moreover, addition of exogenous polyamines to untreated cells raised the cGMP level in a NO-dependent fashion, and enhanced [3H]-thymidine incorporation. The latter effect was inhibited by sGC or PKG inhibitors. Treatment of quiescent cells with NO donors, 8-bromo-cGMP or YC-1, an sGC activator, also promoted DNA synthesis. Furthermore, putrescine and NO donor can additively activate sGC in cell-free extracts., Conclusion: TNFalpha and LPS stimulate DNA synthesis in chick embryo CMs and this effect is mediated by polyamines, NO and intracellular cGMP.
- Published
- 2001
- Full Text
- View/download PDF
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