33 results on '"Frankel, T"'
Search Results
2. P57.03 Cellular Engagement and Interaction in the Tumor Microenvironment (TME) Predicts Response to ICI in Metastatic NSCLC
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Qin, A., primary, Lima, F., additional, Bell, S., additional, Kalemkerian, G., additional, Schneider, B., additional, Ramnath, N., additional, Lew, M., additional, Rao, A., additional, and Frankel, T., additional
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- 2021
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3. On the Fundamental Group of a Compact Minimal Submanifold
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Frankel, T.
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- 1966
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4. Murine-and human-derived autologous organoid/immune cell co-cultures as pre-clinical models of pancreatic ductal adenocarcinoma.
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Adhikary P., Scott A., Kuester R., Steele N., Woodson C., Holokai L., Chakrabarti J., Lundy J., Croagh D., Richards S.S., Zavros Y., Ahmad S.A., Shroff R.T., Wang J., Jenkins B.J., Merchant J., Frankel T., Khreiss M., Adhikary P., Scott A., Kuester R., Steele N., Woodson C., Holokai L., Chakrabarti J., Lundy J., Croagh D., Richards S.S., Zavros Y., Ahmad S.A., Shroff R.T., Wang J., Jenkins B.J., Merchant J., Frankel T., and Khreiss M.
- Abstract
Purpose: Pancreatic ductal adenocarcinoma (PDAC) has the lowest five-year survival rate of all cancers in the United States. Programmed death 1 receptor (PD-1)-programmed death ligand 1 (PD-L1) immune checkpoint inhibition has been unsuccessful in clinical trials. Myeloid-derived suppressor cells (MDSCs) are known to block anti-tumor CD8+ T cell immune responses in various cancers including pancreas. This has led us to our objective that was to develop a clinically relevant in vitro organoid model to specifically target mechanisms that deplete MDSCs as a therapeutic strategy for PDAC. Method(s): Murine and human pancreatic ductal adenocarcinoma (PDAC) autologous organoid/immune cell co-cultures were used to test whether PDAC can be effectively treated with combinatorial therapy involving PD-1 inhibition and MDSC depletion. Result(s): Murine in vivo orthotopic and in vitro organoid/immune cell co-culture models demonstrated that polymorphonuclear (PMN)-MDSCs promoted tumor growth and suppressed cytotoxic T lymphocyte (CTL) proliferation, leading to diminished efficacy of checkpoint inhibition. Mouse-and human-derived organoid/immune cell co-cultures revealed that PD-L1-expressing organoids were unresponsive to nivolumab in vitro in the presence of PMN-MDSCs. Depletion of arginase 1-expressing PMN-MDSCs within these co-cultures rendered the organoids susceptible to anti-PD-1/PD-L1-induced cancer cell death. Conclusion(s): Here we use mouse-and human-derived autologous pancreatic cancer organoid/immune cell co-cultures to demonstrate that elevated infiltration of polymorphonuclear (PMN)-MDSCs within the PDAC tumor microenvironment inhibit T cell effector function, regardless of PD-1/PD-L1 inhibition. We present a pre-clinical model that may predict the efficacy of targeted therapies to improve the outcome of patients with this aggressive and otherwise unpredictable malignancy.Copyright © 2020 by the authors. Licensee MDPI, Basel, Switzerland.
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- 2021
5. Adjuvant Therapy in Distal Cholangiocarcinoma Following Pancreaticoduodenectomy: A National Cancer Database Analysis
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Kamarajah, S., primary, Frankel, T., additional, Bednar, F., additional, Cho, C., additional, and Nathan, H., additional
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- 2021
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6. Essential fatty acid deficiency in the cat (Felis catus L.)
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Frankel, T. L.
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590 ,Zoology - Published
- 1980
7. Validation of the american joint commission on cancer (AJCC) 8th staging edition in patients with pancreatic adenocarcinoma: a SEER database analysis
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Kathir Kamarajah, S., primary, Burns, W., additional, Frankel, T., additional, Cho, C., additional, and Nathan, H., additional
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- 2019
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8. On Theorems of Hurwitz and Bochner
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FRANKEL, T.
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- 1966
9. 10-year stroke prevention after successful carotid endarterectomy for asymptomatic stenosis (ACST-1): a multicentre randomised trial
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Halliday, A, Harrison, M, Hayter, E, Kong, X, Mansfield, A, Marro, J, Pan, H, Peto, R, Potter, J, Rahimi, K, Rau, A, Robertson, S, Streifler, J, Thomas, D, Fraedrich G, Asymptomatic Carotid Surgery Trial Collaborative G. r. o. u. p., Schmidauer, C, Hölzenbein, Th, Huk, I, Haumer, M, Kretschmer, G, Metz, V, Polterauer, P, Teufelsbauer, H, Cras, P, Hendriks, J, Lauwers, P, Van Schil, P, de Souza EB, Dourado, Me, Gurgel, G, Rocha, Gm, Petrov, V, Slabakov, G, Cooper, Me, Gubitz, G, Holness, R, Howes, W, Langille, R, Legg, K, Nearing, S, Mackean, G, Mackay, M, Phillips, Sj, Sullivan, J, Wood, J, Erdelez, L, Sosa, T, Angelides, Ns, Christopoulos, G, Malikidou, A, Pesta, A, Ambler, Z, Mracek, J, Polivka, J, Rohan, V, Sevcik, P, Simaná, J, Benes, V, Kramár, F, Kaste, M, Lepäntalo, M, Soinne, L, Cardon, Jm, Legalou, A, Gengenbach, B, Pfadenhauer, K, Wölfl, Kd, Flessenkämper, I, Klumpp, Bf, Marsch, J, Kolvenbach, R, Pfeiff, T, Sandmann, W, Beyersdorf, F, Hetzel, A, Sarai, K, Schöllhorn, J, Spillner, G, Lutz, Hj, Böckler, D, Maeder, N, Busse, O, Grönniger, J, Haukamp, F, Balzer, K, Knoob, Hg, Roedig, G, Virreira, L, Franke, S, Moll, R, Schneider, J, Dayantas, J, Sechas, Mn, Tsiaza, S, Kiskinis, D, Apor, A, Dzinich, C, Entz, L, Hüttl, K, Jàrànyi, Z, Mogan, I, Nagy, Z, Szabo, A, Varga, D, Juhász, G, Mátyás, L, Hutchinson, M, Mehigan, D, Aladjem, Z, Harah, E, Elmakias, S, Gurvich, D, Yoffe, B, Ben Meir, H, Dagan, L, Karmeli, R, Keren, G, Shimony, A, Weller, B, Avrahami, R, Koren, R, Streifler, Jy, Tabachnik, S, Zelikovski, A, Angiletta, D, Federico, F, Impedovo, G, Marotta, V, Pascazio, L, Regina, G, Andreoli, A, Pozzati, E, Bonardelli, S, Giulini, Sm, Guarneri, B, Caiazzo, P, Mascoli, F, Becchi, G, Masini, R, Santoro, E, Simoni, G, Ventura, M, Scarpelli, P, Spartera, C, Arena, O, Collice, M, Puttini, M, Romani, F, Santilli, I, Segramora, V, Sterzi, R, Deriu, G, Verlato, F, Cao, Pg, Cieri, Enrico, De Rango, P, Moggi, L, Ricci, S, Antico, A, Spigonardo, F, Malferrari, G, Tusini, N, Vecchiati, E, Cavallaro, A, Kasemi, H, Marino, M, Sbarigia, E, Speziale, F, Zinicola, N, Alò, Fp, Bartolini, M, Carbonari, L, Caporelli, S, Grili Cicilioni, C, Lagalla, G, Ioannidis, G, Pagliariccio, G, Silvestrini, M, Palombo, D, Peinetti, F, Adovasio, R, Chiodo Grandi, F, Mase, G, Zamolo, F, Fregonese, V, Gonano, N, Mozzon, L, Blair, R, Chuen, J, Ferrar, D, Garbowski, M, Hamilton, Mj, Holdaway, C, Muthu, S, Shakibaie, F, Vasudevan, Tm, Kroese, A, Slagsvold, Ce, Dahl, T, Johnsen, Hj, Lange, C, Myhre, Ho, Gniadek, J, Andziak, P, Elwertowski, M, Leszczynski, J, Malek, Ak, Mieszkowski, J, Noszczyk, W, Szostek, M, Toutounchi, S, Correia, C, Pereira, Mc, Akchurin, Rs, Flis, V, Miksic, K, Stirn, B, Tetickovic, E, Cairols, M, Capdevila, Jm, Iborra Ortega, E, Obach, V, Riambau, V, Vidal Barraquer, F, Vila Coll, R, Diaz Vidal, E, Iglesias Negreia JI, Tovar Pardo, A, Iglesias, Rj, Alfageme, Af, Barba Velez, A, Estallo Laliena, L, Garcia Monco JC, Gonzalez, Lr, Corominas, C, Julia, J, Lozano, P, Marti Masso JF, Porta, Rm, Carrera, Ar, Gomez, J, Blomstrand, C, Gelin, J, Holm, J, Karlström, L, Mattsson, E, Bornhov, S, Dahlstrom, J, De Pedis, G, Jensen, Sm, Pärsson, H, Plate, G, Qvarfordt, P, Arvidsson, B, Brattström, L, Forssell, C, Potemkowski, A, Skiöldebrand, C, Stoor, P, Blomqvist, M, Calander, M, Lundgren, F, Almqvist, H, Norgren, L, Norrving, B, Ribbe, E, Thörne, J, Gottsäter, A, Mätzsch, T, Nilsson, Me, Lonsson, M, Stahre, B, Stenberg, B, Konrad, P, Jarl, L, Lundqvist, L, Olofsson, P, Rosfors, S, Swedenborg, J, Takolander, R, Bergqvist, D, Ljungman, C, Kniemeyer, Hw, Widmer, Mk, Kuster, R, Kaiser, R, Nagel, W, Sege, D, Weder, B, De Nie, J, Doelman, J, Yilmaz, N, Buth, J, Stultiens, G, Boiten, J, Boon, A, van der Linden, F, Busman, Dc, Sinnige, Ha, Yo, Ti, de Borst GJ, Eikelboom, Bc, Kappelle, Lj, Moll, F, Dortland, Rw, Westra, Te, Jaber, H, Manaa, J, Meftah, Rb, Nabil, Br, Sraieb, T, Bateman, D, Budd, J, Horrocks, M, Kivela, M, Shaw, L, Walker, R, D'Sa, Aa, Fullerton, K, Hannon, R, Hood, Jm, Lee, B, Mcguigan, K, Morrow, J, Reid, J, Soong, Cv, Simms, M, Baird, R, Campbell, M, Cole, S, Ferguson, It, Lamont, P, Mitchell, D, Sassano, A, Smith, Fc, Blake, K, Kirkpatrick, Pj, Martin, P, Turner, C, Clegg, Jf, Crosley, M, Hall, J, De Cossart, L, Edwards, P, Fletcher, D, Rosser, S, Mccollum, Pt, Davidson, D, Levison, R, Bradbury, Aw, Chalmers, Rt, Dennis, M, Murie, J, Ruckley, Cv, Sandercock, P, Campbell, Wb, Frankel, T, Gardner Thorpe, C, Gutowski, N, Hardie, R, Honan, W, Niblett, P, Peters, A, Ridler, B, Thompson, Jf, Bone, I, Welch, G, Grocott, Ec, Overstall, P, Aldoori, Mi, Dafalla, Be, Bryce, J, Clarke, C, Ming, A, Wilkinson, Ar, Bamford, J, Berridge, D, Scott, J, Abbott, Rj, Naylor, R, Harris, P, Humphrey, P, Adiseshiah, M, Aukett, M, Baker, D, Bishop, Cc, Boutin, A, Brown, M, Burke, P, Burnand, Kg, Colchester, A, Coward, L, Davies, Ah, Espasandin, M, Giddings, Ae, Hamilton, G, Judge, C, Kakkos, S, Mcguiness, C, Morris Vincent, P, Nicolaides, A, Padayachee, Ts, Riordan, H, Sullivan, E, Taylor, P, Thompson, M, Wolfe, Jh, Mccollum, Cn, O'Neill, Pa, Welsh, S, Barnes, J, Cleland, P, Davis, M, Gholkar, A, Jones, R, Jaykishnam, V, Mendelow, Ad, O'Connell, Je, Siddique, Ms, Stansby, G, Vivar, R, Ashley, S, Cosgrove, C, Gibson, J, Wilkins, Dc, Chant, Ad, Frankel, J, Shearman, Cp, Williams, J, Hall, G, Holdsworth, R, Davies, Jn, Mclean, B, Woodburn, Kr, Brown, G, Curley, P, Loizou, L, Chaturvedi, S, Diaz, F, Radak, D, Todorovic, Pr, Kamugasha, D, Baxter, A, Berry, C, Burrett, J, Collins, R, Crowther, J, Davies, C, Farrell, B, Godwin, J, Gray, R, Harwood, C, Hirt, L, Hope, C, Knight, S, Lay, M, Munday, A, Murawska, A, Peto, Cg, Radley, A, Richards, S., Cras, Patrick, van Schil, Paul, et al., Asymptomatic Carotid Surgery Trial (ACST) Collaborative Group, Halliday, A, Harrison, M, Hayter, E, Kong, X, Mansfield, A, Marro, J, Pan, H, Peto, R, Potter, J, Rahimi, K, Rau, A, Robertson, S, Streifler, J, Thomas, D, Adovasio, Roberto, and Asymptomatic Carotid Surgery Trial Collaborative, Group
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Male ,Time Factors ,medicine.medical_treatment ,Kaplan-Meier Estimate ,Carotid endarterectomy ,Aged ,80 and over ,Carotid Stenosis ,Endarterectomy ,Carotid ,Female ,Humans ,Incidence ,Middle Aged ,Primary Prevention ,Stroke ,Treatment Outcome ,Stroke/epidemiology ,law.invention ,Randomized controlled trial ,law ,Aged, 80 and over ,Endarterectomy, Carotid ,endarterectomy ,Carotid Stenosis/mortality ,Incidence (epidemiology) ,Carotid*/mortality ,General Medicine ,Carotid Stenosis | Internal Carotid Artery | Endarterectomy ,medicine.symptom ,medicine.medical_specialty ,Asymptomatic ,Internal medicine ,asymptomatic carotid artery stenosi ,medicine ,asymptomatic carotid artery stenosis ,business.industry ,Carotid Stenosis/complications ,Stroke/prevention & control ,Perioperative ,medicine.disease ,Surgery ,Stenosis ,Human medicine ,business - Abstract
SummaryBackgroundIf carotid artery narrowing remains asymptomatic (ie, has caused no recent stroke or other neurological symptoms), successful carotid endarterectomy (CEA) reduces stroke incidence for some years. We assessed the long-term effects of successful CEA.MethodsBetween 1993 and 2003, 3120 asymptomatic patients from 126 centres in 30 countries were allocated equally, by blinded minimised randomisation, to immediate CEA (median delay 1 month, IQR 0·3–2·5) or to indefinite deferral of any carotid procedure, and were followed up until death or for a median among survivors of 9 years (IQR 6–11). The primary outcomes were perioperative mortality and morbidity (death or stroke within 30 days) and non-perioperative stroke. Kaplan-Meier percentages and logrank p values are from intention-to-treat analyses. This study is registered, number ISRCTN26156392.Findings1560 patients were allocated immediate CEA versus 1560 allocated deferral of any carotid procedure. The proportions operated on while still asymptomatic were 89·7% versus 4·8% at 1 year (and 92·1% vs 16·5% at 5 years). Perioperative risk of stroke or death within 30 days was 3·0% (95% CI 2·4–3·9; 26 non-disabling strokes plus 34 disabling or fatal perioperative events in 1979 CEAs). Excluding perioperative events and non-stroke mortality, stroke risks (immediate vs deferred CEA) were 4·1% versus 10·0% at 5 years (gain 5·9%, 95% CI 4·0–7·8) and 10·8% versus 16·9% at 10 years (gain 6·1%, 2·7–9·4); ratio of stroke incidence rates 0·54, 95% CI 0·43–0·68, p
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- 2010
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10. Risk factors for hemorrhage-related reexploration and blood transfusion after conventional versus coronary revascularization without cardiopulmonary bypass
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FRANKEL, T, primary, STAMOU, S, additional, LOWERY, R, additional, KAPETANAKIS, E, additional, HILL, P, additional, HAILE, E, additional, and CORSO, P, additional
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- 2005
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11. Validation of the doubly-labelled water technique in the domestic dog (Canis familiaris)
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Speakman, J. R., primary, Perez-Camargo, G., additional, McCappin, T., additional, Frankel, T., additional, Thomson, P., additional, and Legrand-Defretin, V., additional
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- 2001
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12. Recurrence of hepatocellular carcinoma at surgical incision site: case series and review of literature.
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Barrett, M., Nathan, H., Vankayala, H., Bieliauskas, S. L., Viglianti, B. L., and Frankel, T. L.
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- 2017
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13. On the Growth of Waves on Manifolds
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Driver, B., primary and Frankel, T., additional
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- 1993
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14. Luring the English-speaking world: Hungarian History diverted.
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Frankel, T.
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- VAN Bethlen, Count
- Abstract
Discusses the work of the former Hungarian Prime Minister, Count Istvan Bethlen, who believed that only a rearrangement in European relations could assist in realizing the revisionist aspirations of Hungary. How Count Bethlen set out to restore the integrity of `historical' Hungary; Reviews the pamphlet, `Treaty Revision and the Hungarian Frontiers,' by R.W. Seton-Watson; More.
- Published
- 1991
15. Models for the human brain.
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Crawford, M. A. and Frankel, T.
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- 1980
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16. Protein digestion in rainbow lorikeets, Trichoglossus haematodus.
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Delia, D. and Frankel, T. L.
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- *
RAINBOW lorikeet , *PROTEINS , *TRICHOGLOSSUS , *LORIES , *BIRD food - Abstract
Background -- Rainbow lorikeets are nectarivorous birds whose natural diet is low in protein and relatively high in free amino acids. Protein metabolisability (PM) of egg white (EW) protein is lower in rainbow lorikeets (6.9%) than whole egg protein in white leghorn roosters (13.9%). Objective -- (1) To determine whether PM of other sources of protein fed to lorikeets is higher than that of EW. (2) To compare the general proteolytic activity (GPA) (pepsin) of the proventriculus of the rainbow lorikeet and a granivorous bird, the domestic chicken, Gallus gallus domesticus L. Design -- (1) Five lorikeets were fed one of three diets, an EW diet, an EW and casein hydrolysate (CH) diet and a commercial "lorikeet and honeyeater" (L/H) feed (Wombaroo Food Products, Glen Osmond, SA). Lorikeets were kept in metabolism cages for 3d for feed intake measurements and excreta collection. Samples were freeze dried and analysed for nitrogen. (2) The GPA of the proventriculus of three lorikeets and three chickens was measured using haemoglobin as a substrate. Outcomes -- (1) The PM (mean ± SD, n = 5) was 4.3 ± 2.6% for the EW diet, 5.6 ± 2.5% for the EW/CH diet and 7.3 ± 3.2% for the L/H feed. The PM for the EW diet was not significantly different from the EW/CH diet and L/H feed (P>0.05). (2) The GPA of the proventriculus of lorikeets at pH 1.0 was significantly lower (P<0.01) than that of the chicken at each incubation period. Conclusions -- (1) The results of the feeding experiments with lorikeets confirm that PM of artificial protein sources is low. (2) The GPA of the proventriculus of lorikeets is lower than that of the chicken. This may contribute to the low PM by lorikeets. [ABSTRACT FROM AUTHOR]
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- 2005
17. Heart rate of pet dogs: effects of overweight and exercise.
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Kuruvilla, A. and Frankel, T. L.
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- *
HEART beat , *ANIMAL health , *DOGS , *OBESITY in animals , *EXERCISE , *BODY weight - Abstract
Background - In Australia, about 40% of pet dogs are overweight (1). High fat diets and obesity affect heart function of dogs (2). In people, exercise has been shown to improve heart function. There is no information on body condition and level of exercise on heart rate in pet dogs. Objective - To determine the effects of body condition and level of exercise on heart rate (HR) of pet dogs carrying out a three-stage exercise test. Design - Owners of dogs in the Melbourne metropolitan area volunteered their pets for the study. The exercise test, carried out over a 21 m circuit of four ramps, consisted of three periods of 2 min exercise followed by 2 min rest. Circuit speeds varied with size: dogs less than 47 cm in height ran at 3, 4 and 5 km/h, those above 47 cm ran at 4, 6 and 8 km/h. A Polar heart rate monitor (Kempele, Finland) was used to record HR at rest, during exercise and during recovery. Body condition was assessed using the Purina body condition system (3). Outcomes - Resting HR of smaller dogs (n=16) was significantly (P<0.05) greater, 135.4 ± 25.7 beats/min (bpm), that of larger dogs, 103.3 ± 20.3 bpm (n=32). Although overweight large dogs (n=20) had a significantly (P<0.05) greater HR (111.8 ± 20.3 bpm) than lean dogs (96.4 ± 18.2, n=28), recovery HR (average HR during recovery as a percentage of HR during exercise) of overweight dogs exercised every day was faster that of lean dogs with limited exercise. Conclusions -- Heart function of pet dogs can be affected by body condition and exercise. [ABSTRACT FROM AUTHOR]
- Published
- 2003
18. Characterization of undifferentiated carcinomas of the pancreas with and without osteoclast-like giant cells.
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Mills JN, Gunchick V, McGue J, Qin Z, Kumar-Sinha C, Bednar F, Brown N, Shi J, Udager AM, Frankel T, Zalupski MM, and Sahai V
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- Humans, Male, Female, Aged, Middle Aged, Carcinoma pathology, Carcinoma genetics, Carcinoma mortality, Carcinoma surgery, Sequence Analysis, RNA, Sequence Analysis, DNA, Aged, 80 and over, Pancreatic Neoplasms genetics, Pancreatic Neoplasms pathology, Pancreatic Neoplasms mortality, Pancreatic Neoplasms surgery, Osteoclasts pathology, Giant Cells pathology, Carcinoma, Pancreatic Ductal pathology, Carcinoma, Pancreatic Ductal genetics, Carcinoma, Pancreatic Ductal mortality, Carcinoma, Pancreatic Ductal surgery, Carcinoma, Pancreatic Ductal immunology
- Abstract
Background: Undifferentiated carcinoma (UC) is a rare subtype of pancreatic cancer distinguished from UC with osteoclast-like giant cells (UC-OGC) in 2019, affecting interpretation of literature that does not distinguish these subtypes. We sought to identify translationally relevant differences between these 2 variants and compared with pancreatic ductal adenocarcinoma., Methods: We characterized clinical and multiomic differences between UC (n = 32) and UC-OGC (n = 15) using DNA sequencing, RNA sequencing, and multiplex immunofluorescence and compared these findings with pancreatic ductal adenocarcinoma., Results: Characteristics at diagnosis were similar between UC and UC-OGC, though the latter was more resectable (P = .009). Across all stages, median overall survival was shorter for UC than for UC-OGC (0.4 years vs 10.8 years, respectively; P = .003). This shorter survival was retained after stratification by resection, albeit without statistical significance (1.8 years vs 11.9 years, respectively; P = .08). In a subset of patients with available tissue, the genomic landscape was similar among UC (n = 9), UC-OGC (n = 5), and pancreatic ductal adenocarcinoma (n = 159). Bulk RNA sequencing was deconvoluted and, along with multiplex immunofluorescence in UC (n = 13), UC-OGC (n = 5), and pancreatic ductal adenocarcinoma (n = 16), demonstrated statistically significantly increased antigen-presenting cells, including M2 macrophages and natural killer cells, and decreased cytotoxic and regulatory T cells in UC and UC-OGC vs pancreatic ductal adenocarcinoma. Findings were similar between UC and UC-OGC , except for decreased regulatory T cells in UC-OGC (P = .04)., Conclusions: In this series, UC was more aggressive than UC-OGC, with these variants having more antigen-presenting cells and fewer regulatory T cells than pancreatic ductal adenocarcinoma, suggesting potential for immune-modulating therapies in the treatment of these pancreatic cancer subtypes., (© The Author(s) 2024. Published by Oxford University Press.)
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- 2025
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19. Isolation and characterization of microbiota from human pancreatic tumors and small intestine.
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Awad D, Attebury H, Hong R, Kim K, Zhang L, Bischoff A, deDekker A, Hoostal M, Nieto Carrion JA, Nelson NS, Strayhorn C, Frankel T, di Magliano MP, Lyssiotis CA, Schmidt TM, and Daley D
- Abstract
Pancreatic ductal adenocarcinoma has a unique tumor microbiome and the systemic depletion of bacteria or fungi using antibiotic/antifungal cocktails leads to a decrease in pancreatic tumor burden in mice. However, functional studies remain rare due to the limited availability of clinically relevant microbiota. Here, we describe in detail the isolation of bacteria and fungi from the small intestine and tumor of pancreatic cancer patients at the Rogel Cancer Center. We then further characterized the impact of a newly isolated Klebsiella oxytoca strain ( UMKO1 ) on the pancreatic tumor microenvironment using bacterial genome sequencing, untargeted and targeted metabolomics, as well as an ex vivo tumor transplant system. We found that UMKO1 possesses a gene for the long form of cytidine deaminase, which can inactivate the standard PDAC chemotherapeutic agent gemcitabine. In addition, we found that UMKO1 can produce several indoles when grown in tumor-like conditions, metabolites that can lead to an immune suppressive environment and interfere with therapy outcome. To test this in detail, we assessed changes in immune populations in pancreatic tumor explants upon exposure to the supernatant of UMKO1 and other isolated bacteria grown in tumor Interstitial fluid media (TIFM). We found that while none of the bacterial supernatants changed the abundance of CD8 T cells, granzyme B positive CD8 T cells were the lowest in tumor explants exposed to UMKO1 , and not other isolated Klebsiella species or the non-pathogenic laboratory strain E. coli K12 . In summary, the isolated collection of bacteria and fungi from this study are a valuable toolbox to study the impact of microbiota on pancreatic cancer.
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- 2024
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20. Mouse Models for Pancreatic Ductal Adenocarcinoma are Affected by the cre-driver Used to Promote KRAS G12D Activation.
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Mousavi F, Thompson J, Lau J, Renollet N, Martin MB, McGue J, Hassan O, Frankel T, Shooshtari P, Pin CL, and Bednar F
- Abstract
Background & Aims: The fundamental biology of pancreatic ductal adenocarcinoma has been greatly impacted by the characterization of genetically engineered mouse models that allow temporal and spatial activation of oncogenic KRAS (KRAS
G12D ). One of the most commonly used models involves targeted insertion of a cre-recombinase into the Ptf1a gene. However, this approach disrupts the Ptf1a gene, resulting in haploinsufficiency that likely affects sensitivity to oncogenic KRAS (KRASG12D ). This study aims to determine if Ptf1a haploinsufficiency affected the acinar cell response to KRASG12D before and after induction of pancreatic injury., Methods: We performed morphological and molecular analysis of 3 genetically engineered mouse models that express a tamoxifen-inducible cre-recombinase to activate KrasG12D in acinar cells of the pancreas. The cre-recombinase was targeted to the acinar-specific transcription factor genes, Ptf1a or Mist1/Bhlha15, or expressed within a BAC-derived Elastase transgene. Histological and RNA-seq analyses were used to delineate differences between the models., Results: Up to 2 months after tamoxifen induction of KRASG12D , morphological changes were negligible. However, induction of pancreatic injury by cerulein resulted in widespread PanIN lesions in Ptf1acreERT pancreata within 7 days and maintained for at least 5 weeks post-injury, which was not seen in the models with 2 functional Ptf1a alleles. RNA-sequencing analysis prior to injury induction suggested Ptf1acreERT and Mist1creERT mice have unique profiles of gene expression that predict a differential response to injury. Multiplex analysis of pancreatic tissue confirmed different inflammatory responses between the models., Conclusions: These findings suggest Ptf1a haploinsufficiency in Ptf1acreERT mouse models promotes KRASG12D priming of genes for promotion of pancreatic ductal adenocarcinoma., (Copyright © 2024 The Authors. Published by Elsevier Inc. All rights reserved.)- Published
- 2024
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21. Assessing the presence, concentration, and impacts of trace element contamination in a Chesapeake Bay tributary adjacent to a coal ash landfill (Possum Point, VA).
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Frankel TE, Tyler E, Willmore C, Odhiambo BK, and Giancarlo L
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- Coal Ash analysis, Cadmium analysis, Ecosystem, Bays, Environmental Monitoring methods, Trace Elements analysis, Water Pollutants, Chemical analysis
- Abstract
Coal ash (CA) is an industrial waste product that has been shown to contain several neurotoxic constituents such as cadmium, selenium, mercury, lead, and arsenic. Contaminant-laced leachates enter the environment via seepage, runoff, permitted discharge, or accidental spills from CA storage ponds or landfills which may pose a risk to wildlife residing in receiving waterways. In this study, we assessed 1) the presence and concentration of thirteen trace elements (Al, Ca, Mg, Cr, Cd, As, Se, Pb, Cu, Zn, Mn, Fe, B) in surface water and sediment grab samples using ICP-OES, 2) the temporal variability of trace elements using Pb-210 dated sediment core samples, 3) differences in species diversity using environmental DNA (eDNA) analyses, and 4) the presence and concentration of trace metals in banded killifish (Fundulus diaphanus) epaxial muscle tissue collected from waterways surrounding the Possum Point Power Station (Stafford, VA). Results showed the highest concentrations of As, Cd, Cr, Cu, Fe, Mg, Se, Zn, and B in Quantico Creek (QC) adjacent to the coal ash ponds and elevated average cadmium and zinc concentrations compared to both upstream and downstream locations along the Potomac River. Sediment core profiles and Pb-210 analyses showed historical enrichment of several trace elements in QC beginning after the commissioning of the power plant in 1948. When compared to upstream and downstream sites, species diversity was drastically reduced in Quantico Creek based on eDNA identification. Muscle tissues of banded killifish collected in Quantico Creek displayed increased Al, Cd, and Zn concentrations compared to upstream and downstream sites. Collectively, our results demonstrate the potential impacts of coal ash landfills on aquatic ecosystems and suggest that further research is needed to fully inform risk assessment and remediation efforts., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier Ltd. All rights reserved.)
- Published
- 2023
- Full Text
- View/download PDF
22. Investigating the potential impacts of coal ash runoff on the freshwater Seminole ramshorn snail (Planorbella duryi) under laboratory conditions.
- Author
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Frankel TE, Crowell C, Giancarlo L, Hydorn D, and Odhiambo BK
- Subjects
- Animals, Coal Ash chemistry, Cadmium, Ecosystem, Snails, Coal, Fresh Water, Trace Elements, Metals, Heavy
- Abstract
Coal fly ash is an industrial waste product generated by coal fired powerplants which has been shown to contain elevated concentrations of several toxic trace metals. When stored in landfills or other repositories, these trace metals can enter nearby surface waters via a number of routes including leaching or runoff. Our study examined 1) the presence and concentration of eleven trace elements in a range of lab-created coal ash leachate solutions at neutral pH using ICP-OES, 2) the physiological effects of these leachate solutions on a freshwater gastropod (Planorbella duryi), and 3) the ability of these trace metals to bioaccumulate in the tissues of exposed individuals. As, Cd, Cu, Mg, Mn, and Pb were detected in solutions at increasing concentrations concurrent with ash concentration. Exposure to leachates caused significant delays in embryonic development, reduced juvenile shell growth, decreases in egg and clutch production, and the display of avoidance behaviors. Tissues of exposed snails contained elevated concentrations of As, Cd, Cu, and Cr, with bioconcentration factors 177,550 times higher in cadmium and 85,468 times higher in arsenic in the highest treatment compared to control organisms. Our results highlight the potential harmful effects of coal ash leachates on a novel freshwater invertebrate species using several unique methodologies, providing key information regarding their potential impacts on surrounding aquatic ecosystems., Competing Interests: Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests:Tyler Edward Frankel reports financial support was provided by Morris Animal Foundation., (Copyright © 2022 Elsevier Ltd. All rights reserved.)
- Published
- 2023
- Full Text
- View/download PDF
23. CGAT: Cell Graph ATtention Network for Grading of Pancreatic Disease Histology Images.
- Author
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Baranwal M, Krishnan S, Oneka M, Frankel T, and Rao A
- Subjects
- Adult, Deep Learning, Female, Humans, Male, Middle Aged, Phenotype, Models, Theoretical, Pancreatic Diseases classification, Pancreatic Diseases pathology
- Abstract
Early detection of Pancreatic Ductal Adenocarcinoma (PDAC), one of the most aggressive malignancies of the pancreas, is crucial to avoid metastatic spread to other body regions. Detection of pancreatic cancer is typically carried out by assessing the distribution and arrangement of tumor and immune cells in histology images. This is further complicated due to morphological similarities with chronic pancreatitis (CP), and the co-occurrence of precursor lesions in the same tissue. Most of the current automated methods for grading pancreatic cancers rely on extensive feature engineering involving accurate identification of cell features or utilising single number spatially informed indices for grading purposes. Moreover, sophisticated methods involving black-box approaches, such as neural networks, do not offer insights into the model's ability to accurately identify the correct disease grade. In this paper, we develop a novel cell-graph based Cell-Graph Attention (CGAT) network for the precise classification of pancreatic cancer and its precursors from multiplexed immunofluorescence histology images into the six different types of pancreatic diseases. The issue of class imbalance is addressed through bootstrapping multiple CGAT-nets, while the self-attention mechanism facilitates visualization of cell-cell features that are likely responsible for the predictive capabilities of the model. It is also shown that the model significantly outperforms the decision tree classifiers built using spatially informed metric, such as the Morisita-Horn (MH) indices., Competing Interests: AR has a consulting agreement with Voxel analytics LLC and consults for Genophyll, LLC. MB is currently employed with the Division of Data and Decision Sciences, Tata Consultancy Services, India. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. The funders were not involved in the study design, collection, analysis, and interpretation of data, the writing of this article or the decision to submit it for publication., (Copyright © 2021 Baranwal, Krishnan, Oneka, Frankel and Rao.)
- Published
- 2021
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24. Murine- and Human-Derived Autologous Organoid/Immune Cell Co-Cultures as Pre-Clinical Models of Pancreatic Ductal Adenocarcinoma.
- Author
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Holokai L, Chakrabarti J, Lundy J, Croagh D, Adhikary P, Richards SS, Woodson C, Steele N, Kuester R, Scott A, Khreiss M, Frankel T, Merchant J, Jenkins BJ, Wang J, Shroff RT, Ahmad SA, and Zavros Y
- Abstract
Purpose : Pancreatic ductal adenocarcinoma (PDAC) has the lowest five-year survival rate of all cancers in the United States. Programmed death 1 receptor (PD-1)-programmed death ligand 1 (PD-L1) immune checkpoint inhibition has been unsuccessful in clinical trials. Myeloid-derived suppressor cells (MDSCs) are known to block anti-tumor CD8+ T cell immune responses in various cancers including pancreas. This has led us to our objective that was to develop a clinically relevant in vitro organoid model to specifically target mechanisms that deplete MDSCs as a therapeutic strategy for PDAC. Method : Murine and human pancreatic ductal adenocarcinoma (PDAC) autologous organoid/immune cell co-cultures were used to test whether PDAC can be effectively treated with combinatorial therapy involving PD-1 inhibition and MDSC depletion. Results : Murine in vivo orthotopic and in vitro organoid/immune cell co-culture models demonstrated that polymorphonuclear (PMN)-MDSCs promoted tumor growth and suppressed cytotoxic T lymphocyte (CTL) proliferation, leading to diminished efficacy of checkpoint inhibition. Mouse- and human-derived organoid/immune cell co-cultures revealed that PD-L1-expressing organoids were unresponsive to nivolumab in vitro in the presence of PMN-MDSCs. Depletion of arginase 1-expressing PMN-MDSCs within these co-cultures rendered the organoids susceptible to anti-PD-1/PD-L1-induced cancer cell death. Conclusions : Here we use mouse- and human-derived autologous pancreatic cancer organoid/immune cell co-cultures to demonstrate that elevated infiltration of polymorphonuclear (PMN)-MDSCs within the PDAC tumor microenvironment inhibit T cell effector function, regardless of PD-1/PD-L1 inhibition. We present a pre-clinical model that may predict the efficacy of targeted therapies to improve the outcome of patients with this aggressive and otherwise unpredictable malignancy.
- Published
- 2020
- Full Text
- View/download PDF
25. Biological and pathological activities of interleukin-22.
- Author
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Perusina Lanfranca M, Lin Y, Fang J, Zou W, and Frankel T
- Subjects
- Animals, Autoimmune Diseases complications, Autoimmune Diseases genetics, Autoimmune Diseases immunology, Autoimmune Diseases metabolism, Autoimmunity, Cell Transformation, Neoplastic genetics, Cell Transformation, Neoplastic immunology, Cell Transformation, Neoplastic metabolism, Humans, Inflammation complications, Inflammation etiology, Inflammation metabolism, Interleukins genetics, Neoplasms pathology, Receptors, Interleukin genetics, Receptors, Interleukin metabolism, Signal Transduction, Interleukin-22, Interleukins metabolism, Neoplasms etiology, Neoplasms metabolism
- Abstract
Interleukin (IL)-22, a member of the IL-10 family, is a cytokine secreted by several types of immune cells including IL-22(+)CD4(+) T cells (Th22) and IL-22 expressing innate leukocytes (ILC22). Recent studies have demonstrated that IL-22 is a key component in mucosal barrier defense, tissue repair, epithelial cell survival, and proliferation. Furthermore, accumulating evidence has defined both protective and pathogenic properties of IL-22 in a number of conditions including autoimmune disease, infection, and malignancy. In this review, we summarize the expression and signaling pathway and functional characteristics of the IL-22 and IL-22 receptor axis in physiological and pathological scenarios and discuss the potential to target IL-22 signaling to treat human diseases.
- Published
- 2016
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26. PRC2 Epigenetically Silences Th1-Type Chemokines to Suppress Effector T-Cell Trafficking in Colon Cancer.
- Author
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Nagarsheth N, Peng D, Kryczek I, Wu K, Li W, Zhao E, Zhao L, Wei S, Frankel T, Vatan L, Szeliga W, Dou Y, Owens S, Marquez V, Tao K, Huang E, Wang G, and Zou W
- Subjects
- Cell Movement genetics, Cell Movement immunology, Cell Proliferation genetics, Colonic Neoplasms immunology, Colonic Neoplasms pathology, Epigenesis, Genetic, Humans, Polycomb Repressive Complex 2 immunology, T-Lymphocytes metabolism, Th1 Cells metabolism, Transfection, Chemokines immunology, Colonic Neoplasms genetics, Polycomb Repressive Complex 2 genetics, T-Lymphocytes immunology, Th1 Cells immunology
- Abstract
Infiltration of tumors with effector T cells is positively associated with therapeutic efficacy and patient survival. However, the mechanisms underlying effector T-cell trafficking to the tumor microenvironment remain poorly understood in patients with colon cancer. The polycomb repressive complex 2 (PRC2) is involved in cancer progression, but the regulation of tumor immunity by epigenetic mechanisms has yet to be investigated. In this study, we examined the relationship between the repressive PRC2 machinery and effector T-cell trafficking. We found that PRC2 components and demethylase JMJD3-mediated histone H3 lysine 27 trimethylation (H3K27me3) repress the expression and subsequent production of Th1-type chemokines CXCL9 and CXCL10, mediators of effector T-cell trafficking. Moreover, the expression levels of PRC2 components, including EZH2, SUZ12, and EED, were inversely associated with those of CD4, CD8, and Th1-type chemokines in human colon cancer tissue, and this expression pattern was significantly associated with patient survival. Collectively, our findings reveal that PRC2-mediated epigenetic silencing is not only a crucial oncogenic mechanism, but also a key circuit controlling tumor immunosuppression. Therefore, targeting epigenetic programs may have significant implications for improving the efficacy of current cancer immunotherapies relying on effective T-cell-mediated immunity at the tumor site., (©2015 American Association for Cancer Research.)
- Published
- 2016
- Full Text
- View/download PDF
27. Long-term outcomes of tricuspid valve replacement in the current era.
- Author
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Filsoufi F, Anyanwu AC, Salzberg SP, Frankel T, Cohn LH, and Adams DH
- Subjects
- Adult, Aged, Aged, 80 and over, Bioprosthesis statistics & numerical data, Boston epidemiology, Female, Follow-Up Studies, Heart Valve Prosthesis statistics & numerical data, Heart Valve Prosthesis Implantation adverse effects, Hospital Mortality, Humans, Longitudinal Studies, Male, Middle Aged, Prosthesis Failure, Reoperation statistics & numerical data, Retrospective Studies, Survival Analysis, Thromboembolism etiology, Heart Valve Diseases surgery, Heart Valve Prosthesis Implantation statistics & numerical data, Tricuspid Valve surgery
- Abstract
Background: Regardless of the indication, tricuspid valve replacement (TVR) has historically been associated with high mortality and morbidity. We report the results of our experience in a high-risk patient population with an emphasis on operative mortality, long-term survival, and valve related events according to the type of prosthesis., Methods: Between 1985 and 1999 TVR was performed in 81 patients (isolated n = 25, combined with valve surgery n = 44, combined with CABG or other n = 12). The mean age was 61 years old (range 19-83 years old). Risk factors included New York Heart Association functional class III/IV (n = 73, 90%), reoperation (n = 58, 72%), urgent/emergent indication (n = 62, 76%), and hepatic dysfunction (n = 13, 16%). Mean pulmonary artery pressure was 34 mmHg. Etiology of tricuspid regurgitation was classified as functional (n = 18, 22%) or organic (n = 52, 64%), or failed previous tricuspid valve surgery (n = 11, 14%)., Results: Tricuspid valve replacement was performed with either a bioprosthetic (n = 34, 42%) or mechanical valve (n = 47, 58%). The overall operative mortality was 22% (n = 18). Risk factors for mortality included urgent/emergent status, age greater than 50 years old, functional etiology, and elevated pulmonary artery pressure. Of the 60 survivors, 26 (43%) died during follow up. After univariate analysis, organic etiology was the only predictor of late death (p = 0.01). Kaplan-Meier survival at 2.5, 5, and 10 years was 80%, 60%, and 45% for bioprosthetic, and 84%, 69%, and 59% for mechanical valves, respectively., Conclusions: Patients requiring TVR are typically high-risk with a high-percentage of reoperations, concomitant cardiac procedures, and end-stage functional class. Operative and overall mortality remains high. Heart failure was the predominant cause of early and late deaths, emphasizing importance of timely referral before the development of end-stage cardiac impairment.
- Published
- 2005
- Full Text
- View/download PDF
28. Sulfate incorporation into organic bone matrix of the tibiotarsus of broiler chicks is reduced by excess dietary methionine.
- Author
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Frankel TL
- Subjects
- Animals, Bone Matrix drug effects, Food, Fortified, Random Allocation, Tarsus, Animal drug effects, Tibia drug effects, Bone Matrix metabolism, Chickens metabolism, Methionine administration & dosage, Sulfates metabolism, Tarsus, Animal metabolism, Tibia metabolism, Vitamin A administration & dosage
- Abstract
Two experiments were conducted in broiler chicks to determine whether dietary imbalances of sulfur amino acids (SAA), vitamin A, or interactions between the two nutrients could influence organic bone matrix metabolism measured with L-[35S]-methionine. In the first experiment, in vivo incorporation of 35S into the tibiotarsal bone matrix of 2-wk-old birds was unaffected by vitamin A treatment of 10 and 100 times the requirement when compared with that of birds receiving recommended amounts of vitamin A. However, 35S incorporation was significantly reduced by increasing the SAA concentration of the diet to 1.5 times the requirement relative to lysine. In the second experiment, in vitro incorporation of 35S, derived from L-[35S]-methionine, into bone matrix was reduced in birds consuming a diet containing 1.5 times the methionine requirement relative to lysine (Diet HS) when compared with those receiving .75 (Diet LS), 1.0 (Diet NS), or 1.25 (Diet MS) times the requirement. Birds consuming Diet LS incorporated significantly more 35S into organic bone matrix than birds consuming the other three diets. Although the ratio of SAA to lysine was that recommended (.76:1), on a weight basis the concentration of SAA in diet NS was relatively high (11.48 g/kg diet) compared with the NRC (1984) recommendation of 9.3 g/kg diet. The results show that excess SAA can affect organic bone matrix metabolism and suggest that SAA may play a role in the etiology of tibial dyschondroplasia. They also indicate the importance of distinguishing between nutrient content of the diet expressed as a ratio and that expressed on a weight basis.
- Published
- 1995
- Full Text
- View/download PDF
29. Quantitative SPECT for indium-111-labeled antibodies in the livers of beagle dogs.
- Author
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Leichner PK, Vriesendorp HM, Hawkins WG, Quadri SM, Yang NC, Stinson RL, Loudenslager DM, Frankel TL, Chen XY, and Klein JL
- Subjects
- Animals, Dogs, Antibodies, Indium Radioisotopes, Liver diagnostic imaging, Tomography, Emission-Computed, Single-Photon methods
- Abstract
Results are presented for SPECT computations of liver volumes and 111In-labeled antibody activities in the livers of eight normal beagle dogs. Administered activities ranged from 1 to 2 mCi. SPECT studies were acquired 1 day postinjection using a rotating gamma camera system with elliptical orbits in a 360-degree rotation (128 views, 15 sec/view, 64 x 64 matrices). Uniformity-corrected images were reconstructed by use of the circular harmonic transform algorithm with computer software developed in-house. Liver volumes and activities were computed from transverse slices, 1 pixel (6.25 mm) in thickness. Comparison of SPECT and autopsy data demonstrated that absolute values of percent differences between measured and computed liver volumes ranged from 1.0% to 7.2%. Absolute values of percent differences between autopsy data and computed 111In activities in the liver ranged from 2.3% to 7.5%. These results suggest that quantitative SPECT has the potential of becoming an important tool in clinical trials for determining activities and localization volumes of radiolabeled antibodies directly from radionuclide images.
- Published
- 1991
30. The nutritional and metabolic impact of gamma-linolenic acid (18:3omega6) on cats deprived of animal lipid.
- Author
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Frankel TL and Rivers JP
- Subjects
- Animals, Cats, Dietary Fats metabolism, Fatty Acid Desaturases deficiency, Fatty Acids analysis, Female, Phosphatidylcholines blood, Fats deficiency, Linolenic Acids metabolism
- Abstract
1. The syndrome induced by depriving cats of animal lipid is partially cured by feeding 18:3omega6. This is associated with an increase in levels of 20:3omega6, but not 20:4omega6, in plasma phospholipids. 2. It is concluded that the cat lacks delta5 desaturase activity and has a dietary requirement for 18:3omega6 and possible 20:4omega6.
- Published
- 1978
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- View/download PDF
31. Hypervitaminosis A and calcium-regulating hormones in the rat.
- Author
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Frankel TL, Seshadri MS, McDowall DB, and Cornish CJ
- Subjects
- Acute Disease, Animals, Body Weight drug effects, Bone and Bones drug effects, Calcium blood, Chronic Disease, In Vitro Techniques, Male, Parathyroid Hormone blood, Phosphorus blood, Rats, Rats, Inbred Strains, Vitamin D metabolism, Vitamin D poisoning, Calcium metabolism, Hypervitaminosis A
- Abstract
The effect of vitamin A on calcium-regulating hormones was studied in rats. A single oral dose of 30 mg retinol equivalents (RE) given to adult rats caused no change to serum biologically active parathyroid hormone (bioactive-PTH) concentrations. Bioactive-PTH secretion from rat thyroparathyroid gland complexes was not significantly altered after in vitro incubation with 1.18 X 10(-6) M retinol. Chronically intoxicated rats given 15 mg RE 3 times a week for 6 wk, showed higher osteoclast numbers and lower osteoid than controls. Serum bioactive-PTH was not detectable and serum 25-hydroxyvitamin D (25-OHD) (25.2 +/- 12.5 nmol/L) was significantly (P less than 0.03) lower than controls (43.3 +/- 3.1). In acutely intoxicated rats (60 mg RE/d for 2 d), serum bioactive-PTH levels were significantly lower (0.02 +/- 0.05 ng/ml, P less than 0.03) than in control animals (0.14 +/- 0.08). Lower doses of vitamin A, 7.5 mg RE 3 times a week for 3 wk, suppressed serum bioactive-PTH to undetectable levels but had no significant effect on serum 25-OHD. Serum calcium and 25-OHD levels were significantly lower in vitamin D-intoxicated rats given 7.5 mg RE 3 times a week (ca. 3.16 +/- 0.19 mmol/L; 25-OHD 599.7 +/- 110.6 nmol/L) than vitamin D-intoxicated controls (3.42 +/- 0.17; 789.3 +/- 17.7). These results suggest that hypervitaminosis A can alter the metabolism of calcium-regulating hormones.
- Published
- 1986
- Full Text
- View/download PDF
32. Vitamin D in the nutrition of the cat.
- Author
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Rivers JP, Frankel TL, Juttla S, and Hay AW
- Subjects
- Aging, Animals, Calcinosis chemically induced, Hydroxycholecalciferols blood, Nutritional Requirements, Ultraviolet Rays, Vitamin D Deficiency metabolism, Cats metabolism, Vitamin D metabolism
- Published
- 1979
33. Correlations between thickness of skin-folds and body density in 88 soldiers.
- Author
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PASCALE LR, GROSSMAN MI, SLOANE HS, and FRANKEL T
- Subjects
- Humans, Anthropometry, Body Composition, Military Personnel
- Published
- 1956
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