6 results on '"Guang-Yong Wang"'
Search Results
2. High-throughput discovery of chloroplast and mitochondrial DNA polymorphisms in Brassicaceae species by ORG-EcoTILLING.
- Author
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Chang-Li Zeng, Guang-Yong Wang, Jian-Bo Wang, Gui-Xin Yan, Bi-Yun Chen, Kun Xu, Jun Li, Gui-Zhen Gao, Xiao-Ming Wu, Bo Zhao, and Lei Liu
- Subjects
Medicine ,Science - Abstract
BackgroundInformation on polymorphic DNA in organelle genomes is essential for evolutionary and ecological studies. However, it is challenging to perform high-throughput investigations of chloroplast and mitochondrial DNA polymorphisms. In recent years, EcoTILLING stands out as one of the most universal, low-cost, and high-throughput reverse genetic methods, and the identification of natural genetic variants can provide much information about gene function, association mapping and linkage disequilibrium analysis and species evolution. Until now, no report exists on whether this method is applicable to organelle genomes and to what extent it can be used.Methodology/principal findingsTo address this problem, we adapted the CEL I-based heteroduplex cleavage strategy used in Targeting Induced Local Lesions in Genomes (TILLING) for the discovery of nucleotide polymorphisms in organelle genomes. To assess the applicability and accuracy of this technology, designated ORG-EcoTILLING, at different taxonomic levels, we sampled two sets of taxa representing accessions from the Brassicaceae with three chloroplast genes (accD, matK and rbcL) and one mitochondrial gene (atp6). The method successfully detected nine, six and one mutation sites in the accD, matK and rbcL genes, respectively, in 96 Brassica accessions. These mutations were confirmed by DNA sequencing, with 100% accuracy at both inter- and intraspecific levels. We also detected 44 putative mutations in accD in 91 accessions from 45 species and 29 genera of seven tribes. Compared with DNA sequencing results, the false negative rate was 36%. However, 17 SNPs detected in atp6 were completely identical to the sequencing results.Conclusions/significanceThese results suggest that ORG-EcoTILLING is a powerful and cost-effective alternative method for high-throughput genome-wide assessment of inter- and intraspecific chloroplast and mitochondrial DNA polymorphisms. It will play an important role in evolutionary and ecological biology studies, in identification of related genes associated with agronomic importance such as high yield and improved cytoplasmic quality, and for identifying mitochondrial point mutations responsible for diseases in humans and other animals.
- Published
- 2012
- Full Text
- View/download PDF
3. Numerical Simulation of Underwater Shock Wave Propagation in the Vicinity of Rigid Wall Based on Ghost Fluid Method
- Author
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Yong-Chi Li, Rui-Yuan Huang, Guang-Yong Wang, You-Kai Wang, and Ru-Chao Shi
- Subjects
Shock wave ,Physics ,Article Subject ,Computer simulation ,Mechanical Engineering ,Mechanics ,Geotechnical Engineering and Engineering Geology ,Condensed Matter Physics ,lcsh:QC1-999 ,Exact solutions in general relativity ,Ghost fluid ,Mechanics of Materials ,Numerical tests ,Underwater ,Rigid wall ,lcsh:Physics ,Civil and Structural Engineering - Abstract
This paper presents numerical simulation of underwater shock wave propagation nearby complex rigid wall. The Ghost Fluid Method (GFM) for the treatment of complex rigid wall is developed. The theoretical analysis on the GFM-based algorithm and relevant numerical tests demonstrate that the GFM-based algorithm is first-order accurate as applied to complex rigid wall. A large number of challenging numerical tests show that the GFM-based algorithm is robust and quite simple in various practical problems. The numerical results on shock wave propagation in the vicinity of rigid wall are verified by comparing to exact solution and the results by body-fitted-grid method.
- Published
- 2015
4. High-Throughput Discovery of Chloroplast and Mitochondrial DNA Polymorphisms in Brassicaceae Species by ORG-EcoTILLING
- Author
-
Xiaoming Wu, Jianbo Wang, Chang-li Zeng, Kun Xu, Bo Zhao, Guixin Yan, Guang-Yong Wang, Jun Li, Guizhen Gao, Lei Liu, and Biyun Chen
- Subjects
Linkage disequilibrium ,Plant Science ,medicine.disease_cause ,Genome ,Biochemistry ,Molecular Cell Biology ,Genome Sequencing ,Association mapping ,Energy-Producing Organelles ,Phylogeny ,Genetics ,Electrophoresis, Agar Gel ,Mutation ,Multidisciplinary ,DNA, Chloroplast ,food and beverages ,High-Throughput Nucleotide Sequencing ,Genomics ,Cellular Structures ,Medicine ,Genome, Plant ,Research Article ,Mitochondrial DNA ,Science ,Plant Cell Biology ,Molecular Sequence Data ,Computational biology ,Biology ,Bioenergetics ,Genes, Plant ,Chloroplast ,DNA, Mitochondrial ,Polymorphism, Single Nucleotide ,Species Specificity ,Phylogenetics ,Genome Analysis Tools ,Genes, Chloroplast ,medicine ,Genome-Wide Association Studies ,Gene ,Ecotype ,Organelles ,Base Sequence ,Computational Biology ,Reproducibility of Results ,Subcellular Organelles ,Mutagenesis ,Brassicaceae ,Genetic Polymorphism ,Population Genetics - Abstract
BackgroundInformation on polymorphic DNA in organelle genomes is essential for evolutionary and ecological studies. However, it is challenging to perform high-throughput investigations of chloroplast and mitochondrial DNA polymorphisms. In recent years, EcoTILLING stands out as one of the most universal, low-cost, and high-throughput reverse genetic methods, and the identification of natural genetic variants can provide much information about gene function, association mapping and linkage disequilibrium analysis and species evolution. Until now, no report exists on whether this method is applicable to organelle genomes and to what extent it can be used.Methodology/principal findingsTo address this problem, we adapted the CEL I-based heteroduplex cleavage strategy used in Targeting Induced Local Lesions in Genomes (TILLING) for the discovery of nucleotide polymorphisms in organelle genomes. To assess the applicability and accuracy of this technology, designated ORG-EcoTILLING, at different taxonomic levels, we sampled two sets of taxa representing accessions from the Brassicaceae with three chloroplast genes (accD, matK and rbcL) and one mitochondrial gene (atp6). The method successfully detected nine, six and one mutation sites in the accD, matK and rbcL genes, respectively, in 96 Brassica accessions. These mutations were confirmed by DNA sequencing, with 100% accuracy at both inter- and intraspecific levels. We also detected 44 putative mutations in accD in 91 accessions from 45 species and 29 genera of seven tribes. Compared with DNA sequencing results, the false negative rate was 36%. However, 17 SNPs detected in atp6 were completely identical to the sequencing results.Conclusions/significanceThese results suggest that ORG-EcoTILLING is a powerful and cost-effective alternative method for high-throughput genome-wide assessment of inter- and intraspecific chloroplast and mitochondrial DNA polymorphisms. It will play an important role in evolutionary and ecological biology studies, in identification of related genes associated with agronomic importance such as high yield and improved cytoplasmic quality, and for identifying mitochondrial point mutations responsible for diseases in humans and other animals.
- Published
- 2012
5. Cap-assisted hemoclip application with forward-viewing endoscope for hemorrhage induced by endoscopic sphincterotomy: a prospective case series study.
- Author
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Feng Liu, Guang-Yong Wang, and Zhao-Shen Li
- Abstract
Background: Endoscopic sphincterotomy (ES) is a therapeutic technique developed as an advanced application of endoscopic retrograde cholangiopancreatography (ERCP). An important adverse event associated with this procedure is hemorrhage, which may sometimes be uncontrollable. We sought to examine whether cap-assisted hemoclip application is effective in controlling ES-induced hemorrhage. Methods: In this prospective study, we investigated the outcomes in 10 patients who had uncontrolled ES-induced hemorrhage and were treated by cap-assisted application of hemoclip with a forward-viewing endoscope. Results: Nine of the 10 investigated patients were successfully treated using the cap-assisted hemoclip technique with forward-viewing endoscope, yielding a success rate of 90 %. The patient with hemorrhage non-responsive to hemoclipping required catheter embolization of the bleeding artery after its identification by digital subtraction angiography. One of the 10 patients developed mild pancreatitis after the procedure, but was successfully managed conservatively. Conclusions: Cap-assisted hemoclip application with a forward-viewing endoscope appears to be an effective therapeutic modality for achieving hemostasis in cases of ES-induced hemorrhage, without the occurrence of any severe adverse events; we believe that this method should be considered as an option in the management of ES-induced hemorrhage. [ABSTRACT FROM AUTHOR]
- Published
- 2015
- Full Text
- View/download PDF
6. Cap-assisted hemoclip application with forward-viewing endoscope for hemorrhage induced by endoscopic sphincterotomy: a prospective case series study
- Author
-
Feng Liu, Zhao-Shen Li, and Guang-Yong Wang
- Subjects
Male ,medicine.medical_specialty ,Endoscope ,medicine.medical_treatment ,Cap-assisted endoscopic hemoclip ,Hemorrhage ,Postoperative Hemorrhage ,Sphincterotomy, Endoscopic ,medicine ,Humans ,Prospective Studies ,Endoscopic sphincterotomy ,Embolization ,Adverse effect ,Prospective cohort study ,Aged ,Endoscopic retrograde cholangiopancreatography ,medicine.diagnostic_test ,business.industry ,Hemostasis, Endoscopic ,Gastroenterology ,General Medicine ,Digital subtraction angiography ,Middle Aged ,Surgical Instruments ,medicine.disease ,Surgery ,Endoscopes, Gastrointestinal ,Catheter ,Choledocholithiasis ,Treatment Outcome ,Pancreatitis ,Female ,Radiology ,business ,Research Article - Abstract
Background Endoscopic sphincterotomy (ES) is a therapeutic technique developed as an advanced application of endoscopic retrograde cholangiopancreatography (ERCP). An important adverse event associated with this procedure is hemorrhage, which may sometimes be uncontrollable. We sought to examine whether cap-assisted hemoclip application is effective in controlling ES–induced hemorrhage. Methods In this prospective study, we investigated the outcomes in 10 patients who had uncontrolled ES–induced hemorrhage and were treated by cap-assisted application of hemoclip with a forward-viewing endoscope. Results Nine of the 10 investigated patients were successfully treated using the cap-assisted hemoclip technique with forward-viewing endoscope, yielding a success rate of 90 %. The patient with hemorrhage non-responsive to hemoclipping required catheter embolization of the bleeding artery after its identification by digital subtraction angiography. One of the 10 patients developed mild pancreatitis after the procedure, but was successfully managed conservatively. Conclusions Cap-assisted hemoclip application with a forward-viewing endoscope appears to be an effective therapeutic modality for achieving hemostasis in cases of ES–induced hemorrhage, without the occurrence of any severe adverse events; we believe that this method should be considered as an option in the management of ES–induced hemorrhage.
- Full Text
- View/download PDF
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