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Pancreatic ductal adenocarcinoma (PDAC) is one of the most lethal types of cancer. Various microRNAs have been identified to play an important role in PDAC. The study aimed to explore the role of miR-429 in PDAC.The expression and prognostic value of miR-429 were first analyzed using The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) databases. Next, miR-429 expression was evaluated in the tissues and serum of 90 patients with PDAC. CCK8, SRB, wound healing and transwell assays were used to determine the effect of miR-429 on the proliferation, invasion, and migration of PDAC cells, respectively. Weighted gene co-expression network analysis (WGCNA), correlation analysis, TargetScan, and miRDB databases were used to screen and identify the target genes of miR-429.The results revealed that the expression of miR-429 was downregulated in PDAC tissues and the serum compared with those in normal tissues and the serum of healthy volunteers, respectively. The decreased expression of miR-429 was significantly associated with shorter overall survival. The overexpression of miR-429 significantly inhibited the proliferation, invasion, and migration of PDAC cells. Potential target genes of miR-429 were identified using WGCNA and bioinformatics analysis, and the results showed that cadherin 11 (CDH11), inositol polyphosphate-4-phosphatase type I (INPP4A), laminin gamma 1 (LAMC1), low density lipoprotein receptor-related protein 1 (LRP1), and quaking (QKI) were potential target genes of miR-429 in PDAC. Lower expression of CDH11 and QKI was associated with a more favorable prognosis in patients with PDAC. The overexpression of miR-429 could inhibit the expression of CDH11 and QKI. A nomogram model, involving miR-429, CDH11, and QKI, was subsequently constructed to determine their ability to accurately predict overall and disease-free survival in patients with PDAC.Taken together, miR-429 is involved in the development and progress of PDAC. MiR-429 could be recommended as a prognostic biomarker and therapeutic indicator in PDAC diagnosis.

The aim of this research was to identify and localise re-peat sequences in Korean cattle (Hanwoo), which may be a basis for future genetic studies. To achieve this, a Ha n-woo BAC library containing a total of 150,000 clones with an average size of 130–140 kb was constructed us-ing two different restriction enzymes Eco RI and Hind III. A total of 2,794 clones were sequenced successfully at both 5′ and 3′ terminals using RP2 and T7 primers, ge n-erating 5,588 mate-pairs end sequences amounting to a total of 3.3 × 10

To isolate the microsatellites from the chromosomal DNA of the Korean cattle (Hanwoo) and to use those for the genetic selection, four bacteriophage genomic libraries containing the chromosomal DNA of six Hanwoo steers showing the differences in meat quality and quantity were used. Screening of the genomic libraries using 32 P-radiolabeled 5`-(CA)12-3' nucleotide as a probe, resulted in isolation of about 3,000 positive candidate bacteriophage clones that contain (CA)n-type dinucleotide microsatellites. After confirming the presence of microsatellite in each positive candidate clone by Southern blot analysis, the DNA fragments that include microsatellite and flanking sequences possessing less than 2 kb in size, were subcloned into plasmid vector. Results from the analysis of microsatellite length polymorphism, using twenty-two PCR primers designed from flanking region of each microsatellite DNA, demonstrated that 208 and 210 alleles of HW-YU-MS#3 were closely related to the economic traits such as marbling score, daily gain, backfat thickness and M. longissimus dorsi area in Hanwoo. Interestingly, HW-YU-MS#3 microsatellite was localized in bovine chromosome 17 on which QTLs related to regulation of the body fat content and muscle hypertrophy locus are previously known to exist. Taken together, the results from the present study suggest the possible use of the two alleles as a DNA marker related to economic trait to select the Hanwoo in the future. (Asian-Aust. J. Anim. Sci. 2004. Vol 17, No. 10 : 1329-1333)

This paper considers a scheduling problem for a single burn-in oven in the semiconductor manufacturing industry where the oven is a batch processing machine and each batch processing time is represented by the largest processing time among those of all the jobs contained in the batch. Each job belongs to one of the given number of families. Moreover, the release times of the jobs are different from one another. The objective measure of the problem is the maximum completion time (makespan) of all jobs. A dynamic programming algorithm is proposed in the order of polynomial time complexity for a situation where the number of job families is given (fixed). A computational experiment is performed to compare the time complexity of the proposed algorithm with that of another exact algorithm evaluating all possible job sequences based on batching-dynamic programming (BDP). The results of the experiment show that the proposed algorithm is superior to the other.

This paper considers a multicast routing problem to find the minimum cost tree where the whole communication link delay on each path(route) of the tree is subject to a given delay allowance. The problem is formulated as an integer programming problem by using path variables. An associated problem reduction property is then characterised to reduce the solution space. Moreover, a polynomial time column generation procedure is exploited to solve the associated linear programming relaxation with such solution space reduced. Therefore a branch-and-price algorithm is derived to obtain the optimal integer solution(tree) for the problem. Computational results show that the algorithm can solve practical size problems in a reasonable length of time.

The Aharonov-Bohm (AB) interference patterns in ring-shaped conductors are usually dominated by random features. The amplitude of the oscillations is random from sample to sample and from point to point on the magnetic field axis owing to random scattering of the electron trajectories by impurities within the wires. We report experiments on devices made wet etching and global gates, which have shown major progress towards removing the random features. In loops that exhibit ballistic conductance plateaus and cyclotron orbit trapping at 4.2 K, the random pattern of AB oscillations (observed for T

A new electronic trapping effect has been observed in a small GaAs/Al x Ga 1-x As ring, which takes place when the cyclotron orbits match the loop geometry. The trapping causes peaks in the magnetoresistance R(B) at low fields, and the peak positions were used to study the dispersion relation E n (k) of the one-dimensional magnetoelectric subbands. This allows the ring to be used as a mesoscopic mass spectrometer. The electron effective mass increases with magnetic field by a factor of 50 at field of 0.5 T. General agreement obtains between the experiment and the subband calculations for one-dimensional channels, indicating that true solid-state interferometers are possible

We present the design, fabrication and in-vivo testing of a new micromachined probe for skin cancer detection based on oblique incidence reflectometry (OIR). The device miniaturization and fabrication precision provided by micromachining ensure reliable and repeatable high performance of the probe. The developed probe has been tested in a pre-clinical condition. An overall sensitivity of 100% and specificity of 93% haven been achieved in the classification of malignant and benign skin lesions.

Glandular odontogenic cyst (GOC) is an extremely rare lesion occurring in the jawbones. Sialo-odontogenic cyst was first described as a multicystic lesion resembling a botryoid odontogenic cyst (BOC) or a central mucoepidermoid carcinoma by Padayachee in 1987. In 1988, Gardner used the term "glandular odontogenic cyst" and considered it as a histologic variant of BOC. Most authors agreed that GOC was odontogenic because of the concurrent ball-like epithelial structure, ameloblastoma, squamous odontogenic tumor-like proliferation in its wall, or hyaline bodies in the epithelium lining. Recently, immunohistochemical studies of the cytokeratin profiles have also supported this concept. Its aggressive behavior and the recurrent tendency make it important. A new case of GOC in a 59-year-old male presented as a multilocular radiolucency in the anterior region of the mandible, invading the marrow space by epithelial islands is described with other clinicopathologic features and the literature is briefly discussed.

Liposomes, artificial membranous lipid vesicles, have been used as model structures for biological calcification processes. However, there is no definite conclusion that liposomes can be like matrix vesicles for inducing bone calcification and bone-like tissue formation on primary cultured cells. To determine whether liposomes can promote bone cell growth and mineralization by inducing crystal nucleation, liposomes composed of egg phosphatidylcholine, cholesterol, and bovine brain phosphatidylserine were added to 21-day-old Sprague-Dawley fetal rat calvarial cell cultures from day 1. The aims were to observe proliferation and the phenotype of osteoblasts by measuring cell numbers and alkaline phosphatase (ALP) activity and, when added at confluence, to observe calcification. The data were analyzed with two-way ANOVA. During the 16-day culture period, cell numbers were not significantly affected by liposomes (100 mumol/L). However, ALP activity was significantly inhibited by liposomes (p0.05) at day 16 and thereafter. Calcified particles were detected by von Kossa's method, and were larger and more abundant (p0.05) in the liposomes groups than in the control from days 12-24. This response depended on liposomes dose. These findings suggest that liposomes promote calcification and accelerate the formation of bone-like tissue. Liposomes slightly reduce the expression of the osteoblast phenotype and do not affect cell growth in primary rat osteoblast-enriched cultures.