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Phytohormones, epigenetic regulation and environmental factors regulate fruit ripening but their interplay during strawberry fruit ripening remains to be determined. In this study, bagged strawberry fruit exhibited delayed ripening compared with fruit grown in normal light, correlating with reduced abscisic acid (ABA) accumulation. Transcription of the key ABA catabolism gene, ABA 8′‐hydroxylase FaCYP707A4, was induced in bagged fruit. With light exclusion whole genome DNA methylation levels were up‐regulated, corresponding to a delayed ripening process, while DNA methylation levels in the promoter of FaCYP707A4 were suppressed, correlating with increases in transcript and decreased ABA content. Experiments indicated FaCRY1, a blue light receptor repressed in bagged fruit and FaAGO4, a key protein involved in RNA‐directed DNA methylation, could bind to the promoter of FaCYP707A4. The interaction between FaCRY1 and FaAGO4, and an increased enrichment of FaAGO4 directed to the FaCYP707A4 promoter in fruit grown under light suggests FaCRY1 may influence FaAGO4 to modulate the DNA methylation status of the FaCYP707A4 promoter. Furthermore, transient overexpression of FaCRY1, or an increase in FaCRY1 transcription by blue light treatment, increases the methylation level of the FaCYP707A4 promoter, while transient RNA interference of FaCRY1 displayed opposite phenotypes. These findings reveal a mechanism by which DNA methylation influences ABA catabolism, and participates in light‐mediated strawberry ripening. [ABSTRACT FROM AUTHOR]

Highlights: Cutting path Planning System for wafer images without Mark points in different imaging states. Use the interlayer in the chip region as an auxiliary location to determine the cutting path. The determined cutting path is located in the middle of the streets, away from the chip region. Microscopic imaging is easily affected by the strength of illumination, and the chip surface qualities of different wafers are different. Therefore, wafer images have defects such as uneven brightness distribution, obvious differences in chip region characteristics, etc., which affect the positioning accuracy of the wafer cutting path. For this reason, this thesis proposes an automatic chip-cutting path-planning method in the wafer image of the Glass Passivation Parts (GPPs) process without a mark. First, the wafer image is calibrated for brightness. Then, the template matching algorithm is used to determine the chip region and the center of gravity position of the chip region. We find the position of the geometric feature (interlayer) in the chip region, and the interlayer is used as an auxiliary location to determine the final cutting path. The experiment shows that the image quality can be improved, and chip region features can be highlighted when preprocessing the image with brightness calibration. The results show that the average deviation of the gravity coordinates of the chip region in the x direction is 2.82 pixels. We proceeded by finding the interlayer in the chip region, marking it with discrete points, and using the improved Random Sample Consensus (RANSAC) algorithm to remove the abnormal discrete points and fit the remaining discrete points. The average fitting error is 0.8 pixels, which is better than the least squares method (LSM). The cutting path location algorithm proposed in this paper can adapt to environmental brightness changes and different qualities of chips, accurately and quickly determine the cutting path, and improve the chip cutting yield. [ABSTRACT FROM AUTHOR]

E or pyriminobac-methyl (EPM), a pyrimidine benzoic acid esters herbicide, has a high potential as weedicide; nevertheless, its environmental behaviors are still not well understood. In this study, we systematically investigated, for the first time, the adsorption–desorption, degradation, and leaching behaviors of EPM in agricultural soils from five exemplar sites in China (Phaeozems – S1 ; Anthrosol – S2 ; Ferralsol – S3 ; Alisol – S4 ; Plinthosol – S5) through laboratory simulation experiments. Our results show that the EPM adsorption–desorption results were well fitted by the Freundlich model (R2>0.9999). In the analyzed soils, the Freundlich adsorption (i.e., Kf ads) and desorption (i.e., Kf des) coefficients of EPM varied between 0.85 and 32.22 mg 1-1/n L 1/n kg -1 and 0.78–5.02 mg 1-1/n L 1/n kg -1 , respectively. The mobility of EPM in soils S1 – S5 was categorized as immobile, slightly immobile, highly mobile, slightly mobile, and slightly mobile, respectively. Moreover, the degradation of EPM reflected first-order kinetics, where its half-life ranged between 37.46 and 66.00 d, depending on the environmental conditions, and abiotic degradation was predominant in the degradation of this compound. Overall, the high leaching ability and desorption capacity of EPM were accompanied by a low adsorption capacity, and there were no significant relationships between pH and the leaching rate of EPM in the five types of soils. In contrast, the organic matter content, cation exchange capacity, and soil clay content were the main components responsible for the observed leaching rates. We found that EPM degrades easily, has a high adsorption affinity, and a low mobility in S1 , which results in a low contamination risk for groundwater systems. On the contrary, this compound degrades slowly in S2 , S3 , S4 , and S5 , due to a low adsorption affinity and moderate mobility, which results in a high contamination risk for groundwater systems. Therefore, our results may serve as a reference for evaluating the risks involved in the increasingly wide application of this compound. [ABSTRACT FROM AUTHOR]

In two experiments conducted over a 2-year period, oat straw was ensiled with different levels of perennial ryegrass and wet hulless-barley distillers' grains ( WHDG) to improve utilization efficiency of oat straw as ruminants feed. Firstly, oat straw was ensiled with varying proportions of perennial ryegrass (0, 20, 40 or 60% of fresh weight [FW]). The lactic acid ( LA) and lactic/acetic acid ( LA/ AA) were increased (L, Q, P < 0.01) whereas pH value, propionic acid, butyric acid and volatile fatty acids were decreased (L, Q, P < 0.01) with increasing perennial ryegrass levels. From the perspective of maximum use oat straw, it was suggested that 40% perennial ryegrass inclusions was proper for further study. Then, mixtures of oat straw and perennial ryegrass (60/40) were ensiled with WHDG at 0, 100, 200 or 300 g kg-1 FW (defined as WHDG0, WHDG100, WHDG200 and WHDG300, respectively), the fermentation characteristics and in vitro rumen degradability were analyzed. The dry matter ( DM) and pH value declined (L, Q, P < 0.01), while the LA increased (L, Q, P < 0.01) with the increase of WHDG ratios. The inclusion of WHDG significantly decreased ammonia nitrogen concentrations (L, Q, P < 0.01). The crude protein content linearly increased ( P < 0.01), whereas the acid detergent fiber ( ADF) content linearly decreased ( P < 0.01) with increasing levels of WHDG. The WHDG inclusion significantly increased in vitro neutral detergent fiber degradability (L, Q, P < 0.01) with the highest value in WHDG200. The in vitro DM and ADF degradability had a Q effect ( P < 0.01) in response to increasing WHDG levels. Overall inclusion of WHDG can improve the fermentation and in vitro degradability of mixed silages of oat straw and perennial ryegrass. [ABSTRACT FROM AUTHOR]

Aspergillus fumigatus is a saprophytic mold and an opportunistic pathogen with a broad geographic and ecological distribution. A. fumigatus is the most common etiological agent of aspergillosis, affecting over 8,000,000 individuals worldwide. Due to the rising number of infections and increasing reports of resistance to antifungal therapy, there is an urgent need to understand A. fumigatus populations from local to global levels. However, many geographic locations and ecological niches remain understudied, including soil environments from arctic regions. In this study, we isolated 32 and 52 A. fumigatus strains from soils in Iceland and the Northwest Territories of Canada (NWT), respectively. These isolates were genotyped at nine microsatellite loci and the genotypes were compared with each other and with those in other parts of the world. Though significantly differentiated from each other, our analyses revealed that A. fumigatus populations from Iceland and NWT contained evidence for both clonal and sexual reproductions, and shared many alleles with each other and with those collected from across Europe, Asia, and the Americas. Interestingly, we found one triazole-resistant strain containing the TR34 /L98H mutation in the cyp51A gene from NWT. This strain is closely related to a triazole-resistant genotype broadly distributed in India. Together, our results suggest that the northern soil populations of A. fumigatus are significantly influenced by those from other geographic regions. [ABSTRACT FROM AUTHOR]

The lung is an important site of extramedullary platelet formation, and megakaryocytes in the lung participate in immune responses in addition to platelet production. In acute lung injury and chronic lung injury, megakaryocytes and platelets play a promoting or protective role through different mechanisms. The authors reviewed the role of megakaryocytes and platelets in common clinical lung injuries with different course of disease and different pathogenic factors in order to provide new thinking for the diagnosis and treatment of lung injuries.