13 results on '"Kempa, Sally"'
Search Results
2. Autologous Fat Grafting—A Panacea for Scar Tissue Therapy?
- Author
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Ahmad, Nura, Anker, Alexandra, Klein, Silvan, Dean, Jillian, Knoedler, Leonard, Remy, Katya, Pagani, Andrea, Kempa, Sally, Terhaag, Amraj, and Prantl, Lukas
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HEPATOCYTE growth factor ,VASCULAR endothelial growth factors ,BRAIN-derived neurotrophic factor ,FIBROBLAST growth factors ,SCARS ,WOUND healing - Abstract
Scars may represent more than a cosmetic concern for patients; they may impose functional limitations and are frequently associated with the sensation of itching or pain, thus impacting both psychological and physical well-being. From an aesthetic perspective, scars display variances in color, thickness, texture, contour, and their homogeneity, while the functional aspect encompasses considerations of functionality, pliability, and sensory perception. Scars located in critical anatomic areas have the potential to induce profound impairments, including contracture-related mobility restrictions, thereby significantly impacting daily functioning and the quality of life. Conventional approaches to scar management may suffice to a certain extent, yet there are cases where tailored interventions are warranted. Autologous fat grafting emerges as a promising therapeutic avenue in such instances. Fundamental mechanisms underlying scar formation include chronic inflammation, fibrogenesis and dysregulated wound healing, among other contributing factors. These mechanisms can potentially be alleviated through the application of adipose-derived stem cells, which represent the principal cellular component utilized in the process of lipofilling. Adipose-derived stem cells possess the capacity to secrete proangiogenic factors such as fibroblast growth factor, vascular endothelial growth factor and hepatocyte growth factor, as well as neurotrophic factors, such as brain-derived neurotrophic factors. Moreover, they exhibit multipotency, remodel the extracellular matrix, act in a paracrine manner, and exert immunomodulatory effects through cytokine secretion. These molecular processes contribute to neoangiogenesis, the alleviation of chronic inflammation, and the promotion of a conducive milieu for wound healing. Beyond the obvious benefit in restoring volume, the adipose-derived stem cells and their regenerative capacities facilitate a reduction in pain, pruritus, and fibrosis. This review elucidates the regenerative potential of autologous fat grafting and its beneficial and promising effects on both functional and aesthetic outcomes when applied to scar tissue. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
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3. Health Implications of Lipedema: Analysis of Patient Questionnaires and Population-Based Matched Controls
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Kempa, Sally, primary, Gross, Mascha, additional, Oliinyk, Dmytro, additional, Siegmund, Andreas, additional, Müller, Martina, additional, Prantl, Lukas, additional, and Tews, Hauke C., additional
- Published
- 2024
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4. Preliminary Single-Cell RNA-Sequencing Analysis Uncovers Adipocyte Heterogeneity in Lipedema.
- Author
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Pagani, Andrea, Duscher, Dominik, Kempa, Sally, Ghods, Mojtaba, and Prantl, Lukas
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LIPEDEMA ,FAT cells ,RNA sequencing ,ADIPOSE tissues ,COMPLEMENTARY DNA - Abstract
Background: Despite its increasing incidence and prevalence throughout Western countries, lipedema continues to be a very enigmatic disease, often misunderstood or misdiagnosed by the medical community and with an intrinsic pathology that is difficult to trace. The nature of lipedemic tissue is one of hypertrophic adipocytes and poor tissue turnover. So far, there are no identified pathways responsible, and little is known about the cell populations of lipedemic fat. Methods: Adipose tissue samples were collected from affected areas of both lipedema and healthy participants. For single-cell RNA sequencing analysis, the samples were dissociated into single-cell suspensions using enzymatic digestion and then encapsulated into nanoliter-sized droplets containing barcoded beads. Within each droplet, cellular mRNA was converted into complementary DNA. Complementary DNA molecules were then amplified for downstream analysis. Results: The single-cell RNA-sequencing analysis revealed three distinct adipocyte populations at play in lipedema. These populations have unique gene signatures which can be characterized as a lipid generating adipocyte, a disease catalyst adipocyte, and a lipedemic adipocyte. Conclusions: The single-cell RNA sequencing of lipedemic tissue samples highlights a triad of distinct adipocyte subpopulations, each characterized by unique gene signatures and functional roles. The interplay between these adipocyte subtypes offers promising insights into the complex pathophysiology of lipedema. [ABSTRACT FROM AUTHOR]
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- 2024
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5. Serum Metabolomic Profiling of Patients with Lipedema
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Kempa, Sally, primary, Buechler, Christa, additional, Föh, Bandik, additional, Felthaus, Oliver, additional, Prantl, Lukas, additional, Günther, Ulrich L., additional, Müller, Martina, additional, Derer-Petersen, Stefanie, additional, Sina, Christian, additional, Schmelter, Franziska, additional, and Tews, Hauke C., additional
- Published
- 2023
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6. The effect of radiotherapy on fat engraftment for complete breast reconstruction using lipofilling only
- Author
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Heine, Norbert, Eigenberger, Andreas, Brebant, Vanessa, Kempa, Sally, Seitz, Stephan, Prantl, Lukas, and Kuehlmann, Britta
- Subjects
ddc:610 ,610 Medizin ,Obstetrics and Gynecology ,General Medicine ,skin and connective tissue diseases ,Breast augmentation · Radiotherapy · Breast cancer · Lipofilling · Fat graft · Reconstruction - Abstract
Purpose Lipofilling has been established as a standard technique for contour enhancement following breast reconstruction. However, there is a paucity in current literature regarding the use of this technique for complete reconstruction of the female breast as an alternative to conventional techniques, such as expander or flap-based procedures. In particular, the influence of pre-operative irradiation for successful reconstruction has rarely been examined in published studies. Here, the authors describe their experience with successful fat injection in pre-radiated breasts in comparison with non-pre-radiated patients. Methods In this retrospective study, we examined a total of 95 lipofilling treatments on 26 patients (28 breasts). All of them experienced mastectomy following breast cancer; local breast defects after partial resection of the gland were not included in this study. In total, 47 lipofilling procedures in 12 non-irradiated patients (14 breasts) and 48 procedures in 14 irradiated women (also 14 breasts) were performed. Per session, approximately 297 ± 112 cc of adipose tissue was grafted in group A (no radiotherapy) and approximately 259 ± 93 cc was grafted in group B (radiotherapy). Results Among the group of women without pre-operative radiation, 71% of breast reconstructions limited to lipofilling only showed constant engraftment of fat tissue with a successful reconstructive result, whereas only 21% of the patients with pre-radiated breasts showed complete reconstruction of the breast with a permanent fat in-growth. Conclusion Preoperative radiotherapy significantly impedes successful completion of breast reconstructions planned only by autologous fat transfer. Patients should be selected individually and carefully for complete breast reconstruction using lipofilling only.
- Published
- 2022
7. The LRINEC Score—An Indicator for the Course and Prognosis of Necrotizing Fasciitis?
- Author
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Hoesl, Vanessa, Kempa, Sally, Prantl, Lukas, Ochsenbauer, Kathrin, Hoesl, Julian, Kehrer, Andreas, and Bosselmann, Talia
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ddc:610 ,necrotizing fasciitis ,soft tissue infection ,LRINEC ,debridement ,610 Medizin ,General Medicine - Abstract
Background: The Laboratory Risk Indicator for Necrotizing Fasciitis score (LRINEC) is a simple tool used to support early diagnosis of Necrotizing Fasciitis (NF). The aim of this study was to investigate whether the LRINEC is suitable as a progression and prognosis parameter in patients with NF. Methods: In this retrospective study, laboratory data of 70 patients with NF were analyzed. The LRINEC was calculated for every patient at the time of hospital admission and postoperatively after surgical interventions. Furthermore, the LRINEC was examined as a prognostic factor for survival. Results: The overall lethality of our series was 20 out of 70 (28.6%). A highly significant LRINEC decrease was found for serial debridements. The largest decrease was observed after the first debridement. There was a significant difference between the initial LRINEC of deceased and surviving patients. A cut off value of >6.5 (7 LRINEC points) resulted in an optimal constellation of sensitivity (70%) and specificity (60%) to predict lethality in patients with NF. Conclusions: The LRINEC significantly decreases after surgical debridement. An initial LRINEC equal or greater than seven is an independent prognostic marker for lethality and can help to identify high-risk patients.
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- 2022
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8. Adipose Tissue-Derived Stem Cell Yield Depends on Isolation Protocol and Cell Counting Method
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Prantl, Lukas, Eigenberger, Andreas, Brix, Eva, Kempa, Sally, Baringer, Magnus, and Felthaus, Oliver
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ddc:610 ,QH301-705.5 ,adipocyte viability ,Primary Cell Culture ,610 Medizin ,Mesenchymal Stem Cells ,lipoaspirate ,Flow Cytometry ,plastic surgery ,adipose tissue ,stem cells ,fat grafting ,cell yield ,Article ,Lipectomy ,Tissue and Organ Harvesting ,Humans ,Biology (General) ,Cells, Cultured - Abstract
In plastic surgery, lipofilling is a frequent procedure. Unsatisfactory vascularization and impaired cell vitality can lead to unpredictable take rates in the fat graft. The proliferation and neovascularization inducing properties of adipose tissue-derived stem cells may contribute to solve this problem. Therefore, the enrichment of fat grafts with stem cells is studied intensively. However, it is difficult to compare these studies because many factors—often not precisely described—are influencing the results. Our study summarizes some factors which influence the cell yield like harvesting, isolation procedure and quantification. Stem cells were isolated after liposuction. Quantification was done using a cell chamber, colony counting, or flow cytometry with changes to one parameter, only, for each comparison. Quantification of cells isolated after liposuction at the same harvesting site from the same patient can vary greatly depending on the details of the isolation protocol and the method of quantification. Cell yield can be influenced strongly by many factors. Therefore, a comparison of different studies should be handled with care.
- Published
- 2021
9. Facial Rejuvenation with Concentrated Lipograft—A 12 Month Follow-Up Study
- Author
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Strauss, Catharina, Prantl, Lukas, Brix, Eva, Kempa, Sally, Felthaus, Oliver, Eigenberger, Andreas, Brebant, Vanessa, and Anker, Alexandra
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Adult ,Male ,Nasolabial Fold ,610 Medizin ,Centrifugation ,Transplantation, Autologous ,Article ,Lipectomy ,facial rejuvenation ,Germany ,Adipocytes ,Humans ,Rejuvenation ,lcsh:QH301-705.5 ,Aged ,ddc:610 ,adipose-derived stem cells ,patient’s satisfaction ,fat grafting ,stromal vascular fraction ,Middle Aged ,Plastic Surgery Procedures ,Skin Aging ,Adipose Tissue ,lcsh:Biology (General) ,Patient Satisfaction ,Quality of Life ,Female ,Stromal Cells ,Follow-Up Studies - Abstract
Lipofilling is a popular technique to treat volume loss in aging patients. The isolated adipose tissue is composed of adipocytes and stromal vascular fraction cells, which include adipose-derived stem cells (ASC). We hypothesize that the patient’s wrinkle severity scale (WSS) and patient’s satisfaction on the global aesthetic improvement scale (GAIS) can be improved after using concentrated lipoaspirate. Fourteen patients (54 years ± 11.09 years) with volume loss in the midface area underwent waterjet-assisted liposuction (Human Med AG, Schwerin, Germany). Fat was centrifuged in an ACP Double Syringe (Arthrex GmbH, Munich, Germany) using Rotofix 32A centrifuge (Andreas Hettich, GmbH &, Co.KG, Tuttlingen, Germany). Homogenization was performed using the double syringe and a 1.4 mm female–female luerlock connector. After a second centrifugation, patients received periorbital (PO) and nasolabial (NL) lipografting. ASC count was performed after enzymatical digestion. Vitality of cells was assessed using a resazurin assay. During long-term follow up (12 months, n = 10), we found a high patient’s satisfaction (GAIS 1+/−0.52) and a good improvement of the WSS during short- and long-term follow-up. The ASC count of processed lipoaspirate was 2.1-fold higher than of unprocessed lipoaspirate (p <, 0.001). The difference of ASC in sedimented and simply centrifuged lipoaspirate was also significant (p <, 0.05). Facial rejuvenation with concentrated fat graft offers good results concerning objective aesthetic outcome and patient’s satisfaction.
- Published
- 2021
10. Facial rejuvenation with concentrated lipograft: a 12 month follow-up study
- Author
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Prantl, Lukas, Brix, Eva, Kempa, Sally, Felthaus, Oliver, Eigenberger, Andreas, Brébant, Vanessa, Anker, Alexandra, and Strauss, Catharina
- Subjects
ddc:610 - Published
- 2021
11. Facial Rejuvenation with Concentrated Lipograft—A 12 Month Follow-Up Study
- Author
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Prantl, Lukas, primary, Brix, Eva, additional, Kempa, Sally, additional, Felthaus, Oliver, additional, Eigenberger, Andreas, additional, Brébant, Vanessa, additional, Anker, Alexandra, additional, and Strauss, Catharina, additional
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- 2021
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12. High-resolution Visualization of Intestinal Microcirculation using Ultra-microangiography in Patients with Inflammatory Bowel Disease: A Pilot Study.
- Author
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Albaladejo-Fuertes, Sheila, Jung, Ernst Michael, Büchler, Christa, Gülow, Karsten, Kandulski, Arne, Kempa, Sally, Müller, Martina, and Tews, Hauke Christian
- Subjects
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INFLAMMATORY bowel diseases , *MICROCIRCULATION , *INTESTINES , *FLOW visualization , *INTESTINAL diseases - Abstract
Background & Aims: Ultra-microangiography (UMA) is a novel Doppler technique with optimized wall filtering that provides high sensitivity to low-velocity blood flows and optimized visualization of microcirculation. The aim of this pilot study was to compare intestinal vascularization assessed by color Doppler signals (CDS) and UMA. Methods: We investigated intestinal vascularization using UMA and CDS in 13 patients with confirmed inflammatory bowel disease (IBD). A cohort of 28 patients without structural bowel disease served as the control. Results: Microcirculation and dysregulated microcirculation in patients without and with inflammatory bowel disease can be visualized and quantified using UMA. In 83 % of IBD patients and 76% of non-IBD patients, a high resolution of intestinal perfusion could be achieved using UMA. Conclusions: To the best of our knowledge, this is the first study to investigate intestinal vascularization using UMA in patients with and without structural bowel disease. Quantification and visualization of intestinal vascularization should be further investigated in prospective studies and could help guide our therapy of patients with IBD. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
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13. Think big - Plastic surgical treatment of complicated large sacral ulcerations: A case comparison.
- Author
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Neis JP, Cordesmeyer S, Kempa S, Kisch T, Stang F, Kalousis K, Wenzel E, and Mailānder P
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- Cicatrix complications, Critical Care, Debridement, Humans, Middle Aged, Paraplegia complications, Spinal Cord Injuries complications, Wound Healing, Cicatrix surgery, Pressure Ulcer surgery, Plastic Surgery Procedures methods, Sacrococcygeal Region surgery, Surgical Flaps
- Abstract
Objective: Large pressure ulcers are a well know problem occurring frequently on immobilized patients. They can develop rapidly especially over bony prominences on the elderly, ICU patients and on patients after spinal cord injury. Plastic surgical treatment can be challenging if the defects are large and complications occur like affection of anal region or development of a Marjolin's scar ulcer. Large defects of the sacral region are well known in our university hospital. Common local flaps like gluteal rotation or (double) V-Y advancement flap are often used for the treatment of smaller defects. In special cases these therapies are not sufficient. Rarely we use fillet flap of the lower extremity to cover large sacral defects on patients who were unable to walk before., Subjects and Method: In this case report we demonstrate two relatively young paraplegic patients (49 and 57years old) with large sacral defect wounds. One case occurred in 2017, the other in 2019. After spinal cord injury many years ago both of them developed chronic pressure ulcers of the sacral region. In the case of 2017 a Marjolin's scar ulcer developed as a complication. Both patients had previously lost a leg during the surgical treatment. We used the other remaining leg as a fillet flap in combination with interdisciplinary rectum extirpation for sufficient surgical treatment., Results: In both cases adequate coverage of the sacral defect was achieved after interdisciplinary surgical treatment including rectum extirpation. Fillet flaps were safe, even after necessary surgical revisions. In one of the cases a vacuum wound therapy and several debridements were needed. After rehabilitation the patient of the earlier case is able to fully mobilize himself in everyday life and is even able to use public transport., Conclusion: Using a fillet flap of the lower extremity to cover large sacral ulcers is often the last possibility of surgical treatment. Though many complications can occur, full rehabilitation and social participation is possible after fillet flap surgery even with loss of both legs. Depending on patient's motivation and availability of orthopedic technology like special electric wheel chairs and other tools full mobility can be achieved.
- Published
- 2019
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