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144 results on '"Kong, Lingchao"'

3. Vision detection and feature extraction of molten pool behavior in powder laser cladding

Author

DONG Fangyu, CHEN Yongxiong, KONG Lingchao, LIANG Xiubing, and WANG Kaixin

Subjects
laser cladding, molten pool monitoring, image segmentation, feature extraction, time domain analysis, Materials of engineering and construction. Mechanics of materials, TA401-492
Abstract

Molten pool monitoring is the basis of forming process optimization, playing a pivotal role in improving the formation quality. A machine vision monitoring system was set up for the laser cladding process. Based on the OpenCV software library, a method combining K-means image segmentation and dual threshold Otsu image segmentation was proposed, which realizes the accurate distinction between molten pool and plume with the accuracy of extracting geometric features of molten pool contour up to 95%. By designing an orthogonal experiment of laser cladding Ti-6Al-4V metal powder, typical cladding samples were selected to analyze the width and shape change of the weld pool in time domain, obtaining the fluctuation rule of the weld pool of the cladding layer. The results show that the fluctuation frequency and amplitude of the molten pool are affected by the process parameters and their combinations, and gradually stabilize with the progress of the cladding. Abnormal fluctuations in the molten pool during the cladding process are conducive to the positioning and identification of cladding defects, helping to optimize process routes.

Published
2023
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42. Delta Opioid Peptide Targets Brain Microvascular Endothelial Cells Reducing Apoptosis to Relieve Hypoxia-Ischemic/Reperfusion Injury.

Author

Zhang, Ran, Chen, Meixuan, Deng, Zhongfang, Kong, Lingchao, Shen, Bing, and Zhang, Lesha

Subjects
OPIOID receptors, PEPTIDES, ENDOTHELIAL cells, DENTATE gyrus, OPIOIDS, WESTERN immunoblotting, APOPTOSIS, ASTROCYTES
Abstract

Stroke is one of the leading causes of death. (D-ala2, D-leu5) enkephalin (DADLE) is a synthetic peptide and highly selective delta opioid receptor (δOR) agonist that has exhibited protective properties in ischemia. However, the specific target and mechanism are still unclear. The present study explores the expression of δOR on brain microvascular endothelial cells (BMECs) and whether DADLE could relieve I/R-induced injury by reducing apoptosis. A lateral ventricular injection of DADLE for pretreatment, the neurofunctional behavior score, and TTC staining, were used to evaluate the protective effect of DADLE. Immunofluorescence technology was used to label different types of cells with apoptosis-positive signals to test co-localization status. Primary cultured BMECs were separated and treated with DADLE, accompanied by OGD/R. The CCK-8 test was conducted to evaluate cell viability and TdT-mediated dUTP Nick-end Labelling (TUNEL) staining to test apoptosis levels. The levels of apoptosis-related proteins were analyzed by Western blotting. The co-localization results showed that BMECs, but not astrocytes, microglia, or neurons, presented mostly TUNEL-positive signals, especially in the Dentate gyrus (DG) area of the hippocampus. Either activation of δORs on rats' brains or primary BMECs mainly reduce cellular apoptosis and relieve the injury. Interference with the expression δOR could block this effect. DADLE also significantly increased levels of Bcl-2 and reduced levels of Bax. δOR's expressions can be detected on the BMECs, but not on the HEK293 cells, by Western blotting and IFC. Therefore, DADLE exerts a cytoprotective effect, primarily under hypoxia-ischemic injury/reperfusion conditions, by targeting BMECs to inhibit apoptosis. [ABSTRACT FROM AUTHOR]

Published
2023
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46. The Influence of Main Bearing Parameters on The Bearing Wear in Rotary Compressor

Author

Kong, Lingchao, Zhao, Qingfu, Ren, Liping, Xu, Jia, Cheng, Xiaotong, and Hu, Yusheng

Subjects
contact stress, rotary compressor, finite element method, bearing wear
Abstract

The main bearing and sub bearing which support the crankshaft rotation often have wear in the rotary compressor, and the exceptional wear will cause a series of problems which contain the vibration, noises, frictional power rising and reliability reduced. The process of improving the actual exceptional wear problems of bearings is analyzed. And based on the finite element method (FEM), the results of the original and improved bearings are compared with each other; contact stress is chosen to be used to evaluate the wear condition of bearings. Then the influence of height, diameter and clearance of main bearing on the wear of the bearing is analyzed by the accelerated life test and the FEM simulation, and the feasibility of the bearing contact stress to evaluate the wear condition of bearings is further verified, at the same time it provides a theoretical basis for the design of compressor bearing.

Published
2016

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