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4. An integrated transcriptomics and metabolomics study of the immune response of newly hatched chicks to the cytosine-phosphate-guanine oligonucleotide stimulation

Author

Ouattara, Djomangan Adama, primary, Remolue, Lydie, additional, Becker, Jérémie, additional, Perret, Magali, additional, Bunescu, Andrei, additional, Hennig, Kristin, additional, Biliaut, Emeline, additional, Badin, Annemanuelle, additional, Giacomini, Cesarino, additional, Reynier, Frédéric, additional, Andreoni, Christine, additional, Béquet, Frédéric, additional, Lecine, Patrick, additional, and De Luca, Karelle, additional

Published
2020
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5. EFA6 proteins regulate lumen formation through α-actinin 1

Author

Milanini, Julie, Fayad, Racha, Partisani, Mariagrazia, Lecine, Patrick, Borg, Jean-Paul, Franco, Michel, Luton, Frédéric, MITOYAN, Louciné, Institut de pharmacologie moléculaire et cellulaire (IPMC), Université Nice Sophia Antipolis (1965 - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Centre National de la Recherche Scientifique (CNRS)-Université Côte d'Azur (UCA), Centre de Recherche en Cancérologie de Marseille (CRCM), Aix Marseille Université (AMU)-Institut Paoli-Calmettes, Fédération nationale des Centres de lutte contre le Cancer (FNCLCC)-Fédération nationale des Centres de lutte contre le Cancer (FNCLCC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS)-Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Université Côte d'Azur (UCA), Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut Paoli-Calmettes, and Fédération nationale des Centres de lutte contre le Cancer (FNCLCC)-Fédération nationale des Centres de lutte contre le Cancer (FNCLCC)-Aix Marseille Université (AMU)

Subjects
[SDV] Life Sciences [q-bio], [SDV.CAN] Life Sciences [q-bio]/Cancer, [SDV]Life Sciences [q-bio], Lumen, [SDV.CAN]Life Sciences [q-bio]/Cancer, Contractility, EFA6, ACTN1, Epithelium
Abstract

International audience; A key step of epithelial morphogenesis is the creation of the lumen. Luminogenesis by hollowing proceeds through the fusion of apical vesicles at cell–cell contacts. The small nascent lumens grow through extension, coalescence and enlargement, coordinated with cell division, to give rise to a single central lumen. Here, by using MDCK cells grown in 3D-culture, we show that EFA6A (also known as PSD) participates in luminogenesis. EFA6A recruits α-actinin 1 (ACTN1) through direct binding. In polarized cells, ACTN1 was found to be enriched at the tight junction where it acts as a primary effector of EFA6A for normal luminogenesis. Both proteins are essential for the lumen extension and enlargement, where they mediate their effect by regulating the cortical acto-myosin contractility. Finally, ACTN1 was also found to act as an effector for the isoform EFA6B (also known as PSD4) in the human mammary tumoral MCF7 cell line. EFA6B restored the glandular morphology of this tumoral cell line in an ACTN1-dependent manner. Thus, we identified new regulators of cyst luminogenesis essential for the proper maturation of a newly-formed lumen into a single central lumen.

Published
2018

6. Clinical, virological, and biological parameters associated with outcomes of Ebola virus infection in Macenta, Guinea

Author

Vernet, Marie-Astrid, Reynard, Stéphanie, Fizet, Alexandra, Schaeffer, Justine, Pannetier, Delphine, Guedj, Jeremie, Rives, Max, Georges, Nadia, Garcia-Bonnet, Nathalie, Sylla, Aboubacar I., Grovogui, Péma, Kerherve, Jean-Yves, Savio, Christophe, Savio-Coste, Sylvie, Séverac, Marie-Laure, Zloczewski, Philippe, Linares, Sandrine, Harouna, Souley, Abdoul, Bing M'Lebing, Petitjean, Frederic, Samake, Nenefing, Shepherd, Susan, Kinda, Moumouni, Koundouno, Fara Roger, Joxe, Ludovic, Mateo, Mathieu, Lecine, Patrick, Page, Audrey, Tchamdja, Tang Maleki, Schoenhals, Matthieu, Barbe, Solenne, Simon, Bernard, Tran-Minh, Tuan, Longuet, Christophe, L'Hériteau, François, Baize, Sylvain, Centre Pasteur du Cameroun, Réseau International des Instituts Pasteur (RIIP), Biologie des Infections Virales Émergentes - Biology of Emerging Viral Infections (UBIVE), Centre International de Recherche en Infectiologie - UMR (CIRI), École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut Pasteur [Paris], Laboratoire P4 Jean Mérieux-Inserm [Lyon] (Unité de service 3), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre Européen de Recherche en Virologie et Immunologie [Lyon] (Tour Inserm CERVI), Infection, Anti-microbiens, Modélisation, Evolution (IAME (UMR_S_1137 / U1137)), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris 13 (UP13)-Université Paris Diderot - Paris 7 (UPD7)-Université Sorbonne Paris Cité (USPC), AP-HP - Hôpital Bichat - Claude Bernard [Paris], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Ministère de la santé, Ministère de la Santé [Conakry, Guinea], Alliance for International medical Action (ALIMA), BIOASTER Microbiology Technology Institute [Lyon], Croix rouge française, Fondation Mérieux, Centre de Coordination de Lutte contre les Infections Nosocomiales PARIS NORD (CCLIN PARIS NORD), Centre International de Recherche en Infectiologie (CIRI), École normale supérieure de Lyon (ENS de Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-École normale supérieure de Lyon (ENS de Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut Pasteur [Paris] (IP), and Université Paris 13 (UP13)-Université Paris Diderot - Paris 7 (UPD7)-Université Sorbonne Paris Cité (USPC)-Institut National de la Santé et de la Recherche Médicale (INSERM)

Subjects
Adult, Male, Adolescent, viruses, virus diseases, Infant, Hemorrhagic Fever, Ebola, Middle Aged, Viral Load, Ebolavirus, Prognosis, [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, Cohort Studies, Young Adult, Child, Preschool, Outcome Assessment, Health Care, [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology, Humans, [SDV.IMM]Life Sciences [q-bio]/Immunology, Female, Guinea, Survivors, Clinical Medicine, Child
Abstract

International audience; BACKGROUND. The pathogenesis of Ebola virus (EBOV) disease (EVD) is poorly characterized. The establishment of well-equipped diagnostic laboratories close to Ebola treatment centers (ETCs) has made it possible to obtain relevant virological and biological data during the course of EVD and to assess their association with the clinical course and different outcomes of the disease. METHODS. We were responsible for diagnosing EBOV infection in patients admitted to two ETCs in forested areas of Guinea. The pattern of clinical signs was recorded, and an etiological diagnosis was established by RT-PCR for EBOV infection or a rapid test for malaria and typhoid fever. Biochemical analyses were also performed. RESULTS. We handled samples from 168 patients between November 29, 2014, and January 31, 2015; 97 patients were found to be infected with EBOV, with Plasmodium falciparum coinfection in 18%. Overall mortality for EVD cases was 58%, rising to 86% if P. falciparum was also present. Viral load was higher in fatal cases of EVD than in survivors, and fatal cases were associated with higher aspartate aminotransferase (AST) and alanine aminotransferase (ALT), C-reactive protein (CRP), and IL-6 levels. Furthermore, regardless of outcome, EVD was characterized by higher creatine kinase (CPK), amylase, and creatinine levels than in febrile patients without EVD, with higher blood urea nitrogen (BUN) levels in fatal cases of EVD only. CONCLUSION. These findings suggest that a high viral load at admission is a marker of poor EVD prognosis. In addition, high AST, ALT, CRP, and IL-6 levels are associated with a fatal outcome of EVD. Damage to the liver and other tissues, with massive rhabdomyolysis and, probably, acute pancreatitis, is associated with EVD and correlated with disease severity. Finally, biochemical analyses provide substantial added value at ETCs, making it possible to improve supportive rehydration and symptomatic care for patients. FUNDING. The French Ministry of Foreign Affairs, the Agence Française de Développement, and Institut Pasteur.

Published
2017

8. A modular transcriptional signature identifies phenotypic heterogeneity of human tuberculosis infection

Author

Singhania, Akul, primary, Verma, Raman, additional, Graham, Christine M., additional, Lee, Jo, additional, Tran, Trang, additional, Richardson, Matthew, additional, Lecine, Patrick, additional, Leissner, Philippe, additional, Berry, Matthew P. R., additional, Wilkinson, Robert J., additional, Kaiser, Karine, additional, Rodrigue, Marc, additional, Woltmann, Gerrit, additional, Haldar, Pranabashis, additional, and O’Garra, Anne, additional

Published
2018
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12. Characterization and Genetic Analyses of New Genes Coding for NOD2 Interacting Proteins

Author

Thiébaut, Raphaële, primary, Esmiol, Sophie, additional, Lecine, Patrick, additional, Mahfouz, Batoul, additional, Hermant, Aurelie, additional, Nicoletti, Cendrine, additional, Parnis, Stephane, additional, Perroy, Julie, additional, Borg, Jean-Paul, additional, Pascoe, Leigh, additional, Hugot, Jean-Pierre, additional, and Ollendorff, Vincent, additional

Published
2016
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15. Brucella β 1,2 Cyclic Glucan Is an Activator of Human and Mouse Dendritic Cells

Author

Martirosyan, Anna, primary, Pérez-Gutierrez, Camino, additional, Banchereau, Romain, additional, Dutartre, Hélène, additional, Lecine, Patrick, additional, Dullaers, Melissa, additional, Mello, Marielle, additional, Pinto Salcedo, Suzana, additional, Muller, Alexandre, additional, Leserman, Lee, additional, Levy, Yves, additional, Zurawski, Gerard, additional, Zurawski, Sandy, additional, Moreno, Edgardo, additional, Moriyón, Ignacio, additional, Klechevsky, Eynav, additional, Banchereau, Jacques, additional, Oh, SangKon, additional, and Gorvel, Jean-Pierre, additional

Published
2012
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16. Hook2 is involved in the morphogenesis of the primary cilium

Author

Baron Gaillard, Carole L., primary, Pallesi-Pocachard, Emilie, additional, Massey-Harroche, Dominique, additional, Richard, Fabrice, additional, Arsanto, Jean-Pierre, additional, Chauvin, Jean-Paul, additional, Lecine, Patrick, additional, Krämer, Helmut, additional, Borg, Jean-Paul, additional, and Le Bivic, André, additional

Published
2011
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23. Hematopoietic-specific ß1 tubulin participates in a pathway of platelet biogenesis dependent on the transcription factor NF-E2

Author

Lecine, Patrick, Italiano, Joseph E., Kim, Sang-We, Villeval, Jean-Luc, and Shivdasani, Ramesh A.

Abstract

The cellular and molecular bases of platelet release by terminally differentiated megakaryocytes represent important questions in cell biology and hematopoiesis. Mice lacking the transcription factor NF-E2 show profound thrombocytopenia, and their megakaryocytes fail to produce proplatelets, the microtubule-based precursors of blood platelets. Using mRNA subtraction between normal and NF-E2–deficient megakaryocytes, cDNA was isolated encoding ß1 tubulin, the most divergent ß tubulin isoform. In NF-E2–deficient megakaryocytes, ß1 tubulin mRNA and protein are virtually absent. The expression of ß1 tubulin is exquisitely restricted to platelets and megakaryocytes, where it appears late in differentiation and localizes to microtubule shafts and coils within proplatelets. Restoring NF-E2 activity in a megakaryoblastic cell line or in NF-E2–deficient primary megakaryocytes rescues the expression of ß1 tubulin. Re-expressing ß1 tubulin in isolation does not, however, restore proplatelet formation in the defective megakaryocytes, indicating that other critical factors are required; indeed, other genes identified by mRNA subtraction also encode structural and regulatory components of the cytoskeleton. These findings provide critical mechanistic links between NF-E2, platelet formation, and selected microtubule proteins, and they also provide novel molecular insights into thrombopoiesis.

Published
2000
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24. EFA6 proteins regulate lumen formation through α-actinin 1.

Author

Milanini J, Fayad R, Partisani M, Lecine P, Borg JP, Franco M, and Luton F

Subjects
Animals, Dogs, Guanine Nucleotide Exchange Factors, Humans, MCF-7 Cells, Madin Darby Canine Kidney Cells, Protein Binding, Actinin metabolism, Morphogenesis, Nerve Tissue Proteins metabolism
Abstract

A key step of epithelial morphogenesis is the creation of the lumen. Luminogenesis by hollowing proceeds through the fusion of apical vesicles at cell-cell contacts. The small nascent lumens grow through extension, coalescence and enlargement, coordinated with cell division, to give rise to a single central lumen. Here, by using MDCK cells grown in 3D-culture, we show that EFA6A (also known as PSD) participates in luminogenesis. EFA6A recruits α-actinin 1 (ACTN1) through direct binding. In polarized cells, ACTN1 was found to be enriched at the tight junction where it acts as a primary effector of EFA6A for normal luminogenesis. Both proteins are essential for the lumen extension and enlargement, where they mediate their effect by regulating the cortical acto-myosin contractility. Finally, ACTN1 was also found to act as an effector for the isoform EFA6B (also known as PSD4) in the human mammary tumoral MCF7 cell line. EFA6B restored the glandular morphology of this tumoral cell line in an ACTN1-dependent manner. Thus, we identified new regulators of cyst luminogenesis essential for the proper maturation of a newly-formed lumen into a single central lumen., Competing Interests: Competing interestsThe authors declare no competing or financial interests., (© 2018. Published by The Company of Biologists Ltd.)

Published
2018
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