1. Bronchial gene expression alterations associated with radiological bronchiectasis
- Author
-
Xu, Ke, Diaz, Alejandro A, Duan, Fenghai, Lee, Minyi, Xiao, Xiaohui, Liu, Hanqiao, Liu, Gang, Cho, Michael H, Gower, Adam C, Alekseyev, Yuriy O, Spira, Avrum, Aberle, Denise R, Washko, George R, Billatos, Ehab, and Lenburg, Marc E
- Subjects
Biomedical and Clinical Sciences ,Cardiovascular Medicine and Haematology ,Lung ,Genetics ,2.1 Biological and endogenous factors ,Aetiology ,Humans ,Bronchiectasis ,Bronchi ,Radiography ,Tomography ,X-Ray Computed ,Gene Expression ,DECAMP investigators ,Medical and Health Sciences ,Respiratory System ,Cardiovascular medicine and haematology - Abstract
ObjectivesDiscovering airway gene expression alterations associated with radiological bronchiectasis may improve the understanding of the pathobiology of early-stage bronchiectasis.MethodsPresence of radiological bronchiectasis in 173 individuals without a clinical diagnosis of bronchiectasis was evaluated. Bronchial brushings from these individuals were transcriptomically profiled and analysed. Single-cell deconvolution was performed to estimate changes in cellular landscape that may be associated with early disease progression.Results20 participants have widespread radiological bronchiectasis (three or more lobes). Transcriptomic analysis reflects biological processes associated with bronchiectasis including decreased expression of genes involved in cell adhesion and increased expression of genes involved in inflammatory pathways (655 genes, false discovery rate 0.25). Deconvolution analysis suggests that radiological bronchiectasis is associated with an increased proportion of ciliated and deuterosomal cells, and a decreased proportion of basal cells. Gene expression patterns separated participants into three clusters: normal, intermediate and bronchiectatic. The bronchiectatic cluster was enriched by participants with more lobes of radiological bronchiectasis (p
- Published
- 2023