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1. Shifted, the Drosophila Ortholog of Wnt Inhibitory Factor-1, Controls the Distribution and Movement of Hedgehog

Author

Michael A. Halbisen, Bruno Glise, Alain Vincent, Michèle Crozatier, Steve Wise, Catherine A. Miller, David J. Olson, Seth S. Blair, Institut de Recherches et d'Etudes sur le Monde Arabe et Musulman (IREMAM), Sciences Po Aix - Institut d'études politiques d'Aix-en-Provence (IEP Aix-en-Provence)-Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS), Centre de biologie du développement (CBD), Centre National de la Recherche Scientifique (CNRS)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, Laboratoire Bordelais de Recherche en Informatique (LaBRI), Centre National de la Recherche Scientifique (CNRS)-École Nationale Supérieure d'Électronique, Informatique et Radiocommunications de Bordeaux (ENSEIRB)-Université Sciences et Technologies - Bordeaux 1-Université Bordeaux Segalen - Bordeaux 2, Institut de Génomique Fonctionnelle (IGF), Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Montpellier 2 - Sciences et Techniques (UM2)-Centre National de la Recherche Scientifique (CNRS), Anatomopathologie Haut-Lévêque (ANATOMOPATHOLOGIE), CHU, Clinical Neurology Oxford (CLINICAL NEUROLOGY), Hospital, Departement /u661 : Endocrinologie, Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de synthèse organique (DCSO), École polytechnique (X)-Centre National de la Recherche Scientifique (CNRS), FLAveur, VIsion et Comportement du consommateur (FLAVIC), Etablissement National d'Enseignement Supérieur Agronomique de Dijon (ENESAD)-Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB), Institut de Recherches et d'Etudes sur les Mondes Arabes et Musulmans (IREMAM), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre de Biologie Intégrative (CBI), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Université de Bordeaux (UB)-Centre National de la Recherche Scientifique (CNRS)-École Nationale Supérieure d'Électronique, Informatique et Radiocommunications de Bordeaux (ENSEIRB), École polytechnique (X)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Institut de Recherches et d'Etudes sur le Monde Arabe et Musulman ( IREMAM ), Sciences Po Aix - Institut d'études politiques d'Aix-en-Provence ( IEP Aix-en-Provence ) -Aix Marseille Université ( AMU ) -Centre National de la Recherche Scientifique ( CNRS ), Centre de biologie du développement ( CBD ), Université Paul Sabatier - Toulouse 3 ( UPS ) -Centre National de la Recherche Scientifique ( CNRS ), Laboratoire Bordelais de Recherche en Informatique ( LaBRI ), Centre National de la Recherche Scientifique ( CNRS ) -École Nationale Supérieure d'Électronique, Informatique et Radiocommunications de Bordeaux (ENSEIRB)-Université Sciences et Technologies - Bordeaux 1-Université Bordeaux Segalen - Bordeaux 2, Institut de Génomique Fonctionnelle ( IGF ), Centre National de la Recherche Scientifique ( CNRS ) -Université Montpellier 2 - Sciences et Techniques ( UM2 ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Université Montpellier 1 ( UM1 ) -Université de Montpellier ( UM ), Anatomopathologie Haut-Lévêque ( ANATOMOPATHOLOGIE ), Clinical Neurology Oxford ( CLINICAL NEUROLOGY ), Université Montpellier 1 ( UM1 ) -Université Montpellier 2 - Sciences et Techniques ( UM2 ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Centre National de la Recherche Scientifique ( CNRS ), Laboratoire de synthèse organique ( DCSO ), École polytechnique ( X ) -Centre National de la Recherche Scientifique ( CNRS ), FLAveur, VIsion et Comportement du consommateur ( FLAVIC ), Etablissement National d'Enseignement Supérieur Agronomique de Dijon ( ENESAD ) -Institut National de la Recherche Agronomique ( INRA ) -Université de Bourgogne ( UB ), Université de Toulouse (UT)-Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS)-Centre de Biologie Intégrative (CBI), Université de Toulouse (UT)-Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Université de Bordeaux (UB)-École Nationale Supérieure d'Électronique, Informatique et Radiocommunications de Bordeaux (ENSEIRB)-Centre National de la Recherche Scientifique (CNRS), and Institut National de la Recherche Agronomique (INRA)-Etablissement National d'Enseignement Supérieur Agronomique de Dijon (ENESAD)-Université de Bourgogne (UB)

Subjects
Male, MESH: Signal Transduction, MESH : Molecular Sequence Data, MESH: Sequence Homology, Amino Acid, MESH: Drosophila, MESH : Animals, Genetically Modified, Sequence Homology, MESH : Models, Biological, MESH: Amino Acid Sequence, Wing, MESH: Cholesterol, 0302 clinical medicine, Models, Drosophila Proteins, MESH : Female, MESH: Animals, MESH : Cholesterol, Genetics, 0303 health sciences, biology, MESH : Amino Acid Sequence, Wnt signaling pathway, Signal transducing adaptor protein, MESH : Sequence Homology, Amino Acid, Hedgehog signaling pathway, Cell biology, MESH : Phenotype, Amino Acid, Imaginal disc, Cholesterol, Phenotype, MESH: Repressor Proteins, Drosophila, Female, MESH : Mutation, MESH : Repressor Proteins, MESH : Carrier Proteins, Drosophila melanogaster, Signal transduction, MESH : Heparan Sulfate Proteoglycans, Drosophila Protein, Signal Transduction, MESH: Mutation, animal structures, MESH: Drosophila Proteins, MESH : Male, Molecular Sequence Data, MESH : Wing, Genetically Modified, MESH: Carrier Proteins, MESH: Phenotype, General Biochemistry, Genetics and Molecular Biology, MESH: Animals, Genetically Modified, 03 medical and health sciences, Animals, Humans, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, Amino Acid Sequence, MESH : Drosophila, [ SDV.BBM ] Life Sciences [q-bio]/Biochemistry, Molecular Biology, Molecular Biology, Hedgehog, Alleles, 030304 developmental biology, MESH : Signal Transduction, MESH: Humans, MESH: Molecular Sequence Data, MESH: Alleles, MESH : Humans, MESH: Models, Biological, Cell Biology, MESH : Drosophila Proteins, Biological, MESH: Wing, biology.organism_classification, MESH: Male, Repressor Proteins, Mutation, MESH: Heparan Sulfate Proteoglycans, MESH : Animals, MESH : Alleles, Carrier Proteins, MESH: Female, Heparan Sulfate Proteoglycans, 030217 neurology & neurosurgery, Developmental Biology
Abstract

SummaryWe here identify and characterize an extracellular modulator of Hedgehog signaling in Drosophila, Shifted. Shifted is required for high levels of long-range signaling in the developing wing imaginal disc. Surprisingly, shifted encodes the only Drosophila ortholog of the secreted vertebrate protein Wnt Inhibitory Factor-1 (WIF-1), whose known role is to bind to extracellular Wnts and inhibit their activity. However, Shifted does not regulate Hedgehog signaling by affecting Wingless or Wnt signaling. We show instead that Shifted is a secreted protein that acts over a long distance and is required for the normal accumulation of Hh protein and its movement in the wing. Our data further indicate that Shf interacts with Hh and the heparan sulfate proteoglycans. Therefore, we propose that Shf stabilizes the interaction between Hh and the proteoglycans, an unexpected role for a member of the WIF-1 family.

Published
2005
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2. Efficient PhiC31 integrase-mediated site-specific germline transformation of Anopheles gambiae

Author

Kenneth D. Vernick, Nicolas Puchot, Catherine Bourgouin, Gareth J Lycett, Emilie Pondeville, Amy Lynd, J. M. Meredith, Paul Eggleston, Génétique et Génomique des Insectes vecteurs, Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), Keele University [Keele], Liverpool School of Tropical Medicine (LSTM), Support to E.P. was from an ANR-07-MIME-O25-01 award to C.B., from the Roux Foundation (Pasteur Institute) and from a UE323173 Anopath award to K.D.V., to C.B. from award no. ANR-10-LABX-62-IBEID, to P.E. from a Wellcome Trust Programme grant (0084582) and the EU FP7 (INFRAVEC), and to G.J.L. from European Commission grant no. 223736 (TransMalariaBloc) and a Biotechnology and Biological Sciences Research Council grant (no. BB/F021933/1)., We are grateful to the Photography Department of Institut Pasteur, especially F. Gardy and J.-M. Panaud for the production of the video for embryo alignment, and to M. Calos for pET11-ΦC31 and original attP and attB constructs. We thank A. James, A. Handler and N. Windbichler for helpful technical discussions., ANR-10-LABX-0062,IBEID,Integrative Biology of Emerging Infectious Diseases(2010), European Project: 323173,EC:FP7:ERC,ERC-2012-ADG_20120314,ANOPATH(2013), European Project: 228421,EC:FP7:INFRA,FP7-INFRASTRUCTURES-2008-1,INFRAVEC(2009), European Project: 223736,EC:FP7:HEALTH,FP7-HEALTH-2007-B,TRANSMALARIABLOC(2008), Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), Institut Pasteur [Paris]-Centre National de la Recherche Scientifique ( CNRS ), Liverpool School of Tropical Medicine ( LSTM ), ANR-10-LABX-62-IBEID,IBEID,Laboratoire d'Excellence 'Integrative Biology of Emerging Infectious Diseases' ( 2010 ), European Project : 323173,EC:FP7:ERC,ERC-2012-ADG_20120314,ANOPATH ( 2013 ), European Project : 228421,EC:FP7:INFRA,FP7-INFRASTRUCTURES-2008-1,INFRAVEC ( 2009 ), European Project : 223736,EC:FP7:HEALTH,FP7-HEALTH-2007-B,TRANSMALARIABLOC ( 2008 ), and Centre National de la Recherche Scientifique ( CNRS ) -Institut Pasteur [Paris]

Subjects
MESH : RNA, Messenger, MESH: Integrases, [ SDV.BA.ZI ] Life Sciences [q-bio]/Animal biology/Invertebrate Zoology, Anopheles gambiae, MESH : Animals, Genetically Modified, Messenger/metabolism, Genome, Germline, Animals, Genetically Modified, Genetic Engineering/*methods, Plasmid, MESH: Animals, Genetics, [ SDV.SPEE ] Life Sciences [q-bio]/Santé publique et épidémiologie, 3. Good health, Genetically modified organism, Transformation, MESH : Anopheles, Larva, MESH: Genetic Engineering, Genetic Engineering, Functional genomics, [ SDV.MP.PAR ] Life Sciences [q-bio]/Microbiology and Parasitology/Parasitology, Transgene, Genetically Modified, MESH: Transformation, Genetic, Biology, Integrases/*genetics, General Biochemistry, Genetics and Molecular Biology, MESH : Genetic Engineering, MESH: Animals, Genetically Modified, MESH: Anopheles, MESH : Integrases, Transformation, Genetic, Genetic, Anopheles, parasitic diseases, Animals, [SDV.MP.PAR]Life Sciences [q-bio]/Microbiology and Parasitology/Parasitology, RNA, Messenger, Anopheles/*genetics, MESH: RNA, Messenger, MESH : Larva, Integrases, fungi, Larva/genetics, biology.organism_classification, [SDV.BA.ZI]Life Sciences [q-bio]/Animal biology/Invertebrate Zoology, Transformation (genetics), RNA, [SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie, MESH : Animals, MESH : Transformation, Genetic, MESH: Larva
Abstract

International audience; Current transgenic methodology developed for mosquitoes has not been applied widely to the major malaria vector Anopheles gambiae, which has proved more difficult to genetically manipulate than other mosquito species and dipteran insects. In this protocol, we describe PhiC31-mediated site-specific integration of transgenes into the genome of A. gambiae. The PhiC31 system has many advantages over 'classical' transposon-mediated germline transformation systems, because it allows integration of large transgenes at specific, characterized genomic locations. Starting from a general protocol, we have optimized steps from embryo collection to co-injection of transgene-containing plasmid and in vitro-produced PhiC31 integrase mRNA. We also provide tips for screening transgenic larvae. The outlined procedure provides robust transformation in A. gambiae, resulting in homozygous transgenic lines in approximately 2-3 months.

Published
2014
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3. Genes involved in sex pheromone discrimination in Drosophila melanogaster and their background-dependent effect

Author

Jean-François Ferveur, Ralph J. Greenspan, Stéphane Fraichard, Benjamin Houot, Centre des Sciences du Goût et de l'Alimentation [Dijon] (CSGA), Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Centre National de la Recherche Scientifique (CNRS), Department of Biology, West Virginia University, Kavli Institute for Brain and Mind, University of California [San Diego] (UC San Diego), University of California-University of California, This work was supported by Le Centre National de la Recherche Scientifique, the Burgundy Regional Council, the ANR (INSAVEL) to J-FF, and a National Science Foundation grant IOS-0840717 to RJG., Centre des Sciences du Goût et de l'Alimentation [Dijon] ( CSGA ), Institut National de la Recherche Agronomique ( INRA ) -Université de Bourgogne ( UB ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Centre National de la Recherche Scientifique ( CNRS ), and University of California [San Diego] ( UC San Diego )

Subjects
Male, MESH: Olfactory Perception, [ SDV.AEN ] Life Sciences [q-bio]/Food and Nutrition, MESH : Animals, Genetically Modified, lcsh:Medicine, Genes, Insect, MESH: Genes, Insect, Breeding, MESH : Behavior, Animal, MESH: Reproduction, Courtship, Animals, Genetically Modified, Sexual Behavior, Animal, 0302 clinical medicine, MESH : Drosophila melanogaster, MESH: Behavior, Animal, MESH : Female, MESH: Animals, Mating, Sex Attractants, MESH: Sexual Behavior, Animal, 10. No inequality, lcsh:Science, media_common, Genetics, 0303 health sciences, Multidisciplinary, Ecology, Behavior, Animal, Reproduction, MESH : Genes, Insect, Animal Models, MESH : Reproduction, Sensory Systems, Drosophila melanogaster, MESH: Sex Attractants, Mate choice, Sex pheromone, Alimentation et Nutrition, Female, MESH : Mutation, Research Article, MESH: Mutation, media_common.quotation_subject, MESH : Breeding, MESH : Male, MESH: Courtship, Context (language use), MESH: Breeding, Biology, MESH: Drosophila melanogaster, MESH: Animals, Genetically Modified, 03 medical and health sciences, Model Organisms, Species Specificity, MESH : Olfactory Perception, Food and Nutrition, Animals, MESH : Species Specificity, MESH: Species Specificity, Allele, MESH : Sexual Behavior, Animal, 030304 developmental biology, Evolutionary Biology, MESH : Sex Attractants, Genetically Modified, Behavior, Animal, Genes, Insect, Mutation, Olfactory Perception, Sexual Behavior, MESH : Courtship, lcsh:R, biology.organism_classification, MESH: Male, lcsh:Q, MESH : Animals, MESH: Female, [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition, 030217 neurology & neurosurgery, Neuroscience
Abstract

International audience; Mate choice is based on the comparison of the sensory quality of potential mating partners, and sex pheromones play an important role in this process. In Drosophila melanogaster, contact pheromones differ between male and female in their content and in their effects on male courtship, both inhibitory and stimulatory. To investigate the genetic basis of sex pheromone discrimination, we experimentally selected males showing either a higher or lower ability to discriminate sex pheromones over 20 generations. This experimental selection was carried out in parallel on two different genetic backgrounds: wild-type and desat1 mutant, in which parental males showed high and low sex pheromone discrimination ability respectively. Male perception of male and female pheromones was separately affected during the process of selection. A comparison of transcriptomic activity between high and low discrimination lines revealed genes not only that varied according to the starting genetic background, but varied reciprocally. Mutants in two of these genes, Shaker and quick-to-court, were capable of producing similar effects on discrimination on their own, in some instances mimicking the selected lines, in others not. This suggests that discrimination of sex pheromones depends on genes whose activity is sensitive to genetic context and provides a rare, genetically defined example of the phenomenon known as "allele flips," in which interactions have reciprocal effects on different genetic backgrounds.

Published
2012
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4. Complementary function and integrated wiring of the evolutionarily distinct Drosophila olfactory subsystems

Author

Raphael Rytz, Liliane Abuin, Richard Benton, Ana F. Silbering, Yael Grosjean, Gregory S.X.E. Jefferis, Pavan Ramdya, Center for Integrative Genomics - Institute of Bioinformatics, Génopode (CIG), Swiss Institute of Bioinformatics [Lausanne] (SIB), Université de Lausanne (UNIL)-Université de Lausanne (UNIL), Centre des Sciences du Goût et de l'Alimentation [Dijon] (CSGA), Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Centre National de la Recherche Scientifique (CNRS), Medical Research Council Laboratory of Molecular Biology, Centre intégratif de génomique, Université de Lausanne (UNIL), Université de Lausanne ( UNIL ), Centre des Sciences du Goût et de l'Alimentation [Dijon] ( CSGA ), Institut National de la Recherche Agronomique ( INRA ) -Université de Bourgogne ( UB ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Centre National de la Recherche Scientifique ( CNRS ), and Centre National de la Recherche Scientifique (CNRS)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)

Subjects
MESH: Olfactory Perception, histology growth, MESH: Drosophila, receptor, Chemosensory Receptors, MESH : Animals, Genetically Modified, Ligands, Receptors, Odorant, Protein-Coupled Receptors, ligand, Animals, Genetically Modified, MESH : Maze Learning, 0302 clinical medicine, MESH : Biological Evolution, Odorant Receptors, MESH: Ligands, Drosophila Proteins, MESH: Receptors, Odorant, animal, MESH: Animals, Receptor, Antennal Lobe, 0303 health sciences, MESH : Ligands, MESH: Receptors, Ionotropic Glutamate, biology, General Neuroscience, MESH : Receptors, Ionotropic Glutamate, Articles, Olfactory Pathways, MESH : Odors, Olfactory transduction, development physiology, medicine.anatomical_structure, Local Interneurons, Drosophila, [SDV.NEU]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC], MESH : Mutation, In-Vivo, Evolutionary Robotics, maze learning physiology, Ionotropic effect, anatomy, MESH: Mutation, odor, MESH: Drosophila Proteins, Sensory system, glutamate, MESH: Biological Evolution, biological evolution, drosophila genetic, physiology, mutation, olfactory pathway, ionotropic, odorant, Receptors, Ionotropic Glutamate, Olfactory Receptor Neurons, MESH: Animals, Genetically Modified, 03 medical and health sciences, MESH : Olfactory Perception, MESH : Receptors, Odorant, medicine, Animals, Biological Evolution, Drosophila/genetics, Drosophila Proteins/agonists, Drosophila Proteins/genetics, Maze Learning/physiology, Mutation, Odors, Olfactory Pathways/anatomy & histology, Olfactory Pathways/growth & development, Olfactory Perception/genetics, Olfactory Perception/physiology, Olfactory Receptor Neurons/anatomy & histology, Olfactory Receptor Neurons/growth & development, Receptors, Ionotropic Glutamate/agonists, Receptors, Ionotropic Glutamate/genetics, Receptors, Odorant/genetics, Receptors, Odorant/physiology, MESH : Drosophila, Maze Learning, Ionotropic Glutamate Receptors, 030304 developmental biology, Mosquito Anopheles-Gambiae, Communication, Malaria Mosquito, MESH: Odors, MESH : Olfactory Pathways, business.industry, MESH: Maze Learning, MESH: Olfactory Receptor Neurons, biology.organism_classification, Olfactory Perception, MESH : Drosophila Proteins, [ SDV.NEU ] Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC], Odorants, MESH : Olfactory Receptor Neurons, Antennal lobe, Neuron, MESH : Animals, business, Neuroscience, 030217 neurology & neurosurgery, Function (biology), MESH: Olfactory Pathways, Male Courtship Behavior
Abstract

author can archive publisher's version/PDF (embargo 6 months) ; 1529-2401 (Electronic ISSN); International audience; To sense myriad environmental odors, animals have evolved multiple, large families of divergent olfactory receptors. How and why distinct receptor repertoires and their associated circuits are functionally and anatomically integrated is essentially unknown. We have addressed these questions through comprehensive comparative analysis of the Drosophila olfactory subsystems that express the ionotropic receptors (IRs) and odorant receptors (ORs). We identify ligands for most IR neuron classes, revealing their specificity for select amines and acids, which complements the broader tuning of ORs for esters and alcohols. IR and OR sensory neurons exhibit glomerular convergence in segregated, although interconnected, zones of the primary olfactory center, but these circuits are extensively interdigitated in higher brain regions. Consistently, behavioral responses to odors arise from an interplay between IR- and OR-dependent pathways. We integrate knowledge on the different phylogenetic and developmental properties of these receptors and circuits to propose models for the functional contributions and evolution of these distinct olfactory subsystems.

Published
2011
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5. The consequences of regulation of desat1 expression for pheromone emission and detection in Drosophila melanogaster

Author

François Bousquet, Benjamin Houot, Jean-François Ferveur, Centre des Sciences du Goût et de l'Alimentation [Dijon] ( CSGA ), Institut National de la Recherche Agronomique ( INRA ) -Université de Bourgogne ( UB ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Centre National de la Recherche Scientifique ( CNRS ), Centre des Sciences du Goût et de l'Alimentation [Dijon] (CSGA), and Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Centre National de la Recherche Scientifique (CNRS)

Subjects
Fatty Acid Desaturases, Male, Transcription, Genetic, [ SDV.AEN ] Life Sciences [q-bio]/Food and Nutrition, MESH : Animals, Genetically Modified, MESH : Genotype, MESH: Genotype, Animals, Genetically Modified, Sexual Behavior, Animal, MESH : Hydrocarbons, MESH: Reverse Transcriptase Polymerase Chain Reaction, MESH : Drosophila melanogaster, Drosophila Proteins, MESH: Animals, MESH : Female, MESH: Sexual Behavior, Animal, Sex Attractants, Genetics, MESH: Nursing Assessment, MESH : Craniocerebral Trauma, biology, MESH : Gene Expression Regulation, Reverse Transcriptase Polymerase Chain Reaction, MESH : Fatty Acid Desaturases, MESH : Reverse Transcriptase Polymerase Chain Reaction, MESH: Fatty Acid Desaturases, MESH: Gene Expression Regulation, Phenotype, MESH: Intracranial Pressure, MESH: Sex Attractants, Drosophila melanogaster, Sex pheromone, Pheromone, Female, MESH : Mutation, MESH: Mutation, Genotype, MESH : Coma, MESH: Drosophila Proteins, MESH : Male, MESH: Craniocerebral Trauma, Sensory system, Locus (genetics), Investigations, MESH: Drosophila melanogaster, MESH: Animals, Genetically Modified, MESH: Hydrocarbons, MESH: Education, Nursing, Continuing, MESH : Nursing Assessment, Animals, MESH : Sexual Behavior, Animal, Gene, MESH: Coma, Transcriptional activity, MESH : Sex Attractants, MESH: Humans, MESH: Transcription, Genetic, MESH : Humans, MESH : Transcription, Genetic, biology.organism_classification, MESH : Drosophila Proteins, MESH: Male, Hydrocarbons, MESH : Intracranial Pressure, Gene Expression Regulation, Mutation, MESH : Animals, MESH : Education, Nursing, Continuing, MESH: Female, [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition
Abstract

Sensory communication depends on the precise matching between the emission and the perception of sex- and species-specific signals; understanding both the coevolutionary process and the genes involved in both production and detection is a major challenge. desat1 determines both aspects of communication—a mutation in desat1 simultaneously alters both sex pheromone emission and perception in Drosophila melanogaster flies. We investigated whether the alteration of pheromonal perception is a consequence of the altered production of pheromones or if the two phenotypes are independently controlled by the same locus. Using several genetic tools, we were able to separately manipulate the two pheromonal phenotypes, implying that desat1 is the sole gene responsible, exerting a pleiotropic effect on both transmission and detection. The levels of the five desat1 trancripts, measured in the head and body of manipulated flies, were related to variation in pheromone production. This suggests that the pleiotropic action of desat1 on pheromonal communication depends on the fine regulation of its transcriptional activity.

Published
2010
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6. Expression of human CD81 differently affects host cell susceptibility to malaria sporozoites depending on the Plasmodium species

Author

Geert-Jan van Gemert, Jean-François Franetich, Laurent Hannoun, Anne Dubart-Kupperschmitt, Shoshana Levy, Olivier Silvie, Eric Rubinstein, Claude Boucheix, Dominique Mazier, Céline Greco, Robert W. Sauerwein, Immunobiologie Cellulaire et Moléculaire des Infections Parasitaires, Université Pierre et Marie Curie - Paris 6 (UPMC)-IFR113-Institut National de la Santé et de la Recherche Médicale (INSERM), Microenvironnement et Physiopathologie de la Differenciation, Université Paris-Sud - Paris 11 (UP11)-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut Cochin (UMR_S567 / UMR 8104), Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), CHU Pitié-Salpêtrière [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Department of Medical Microbiology, Radboud University Medical Center [Nijmegen], Division of Oncology, Department of Medicine, Stanford University Medical Center, Service de Chirurgie Digestive, Hépato-Bilio-pancréatique et Transplantation Hépatique [CHU Pitié-Salpétrière], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Garcia, Marie, Université Pierre et Marie Curie - Paris 6 ( UPMC ) -IFR113-Institut National de la Santé et de la Recherche Médicale ( INSERM ), Université Paris-Sud - Paris 11 ( UP11 ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ), Institut Cochin ( UMR_S567 / UMR 8104 ), Université Paris Descartes - Paris 5 ( UPD5 ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Centre National de la Recherche Scientifique ( CNRS ), Service de chirurgie digestive et hépato-bilio-pancréatique [CHU Pitié-Salpétrière], and Assistance publique - Hôpitaux de Paris (AP-HP)-CHU Pitié-Salpêtrière [APHP]

Subjects
MESH : Sporozoites, [SDV.BIO]Life Sciences [q-bio]/Biotechnology, MESH : Animals, Genetically Modified, Host tropism, MESH: Sporozoites, MESH: Host-Parasite Relations, Plasmodium, Animals, Genetically Modified, Mice, Tetraspanin, MESH : Plasmodium falciparum, MESH: Animals, [INFO.INFO-BT]Computer Science [cs]/Biotechnology, MESH: Antigens, CD, MESH: Plasmodium falciparum, MESH: Plasmodium yoelii, MESH : Cell Nucleus, 0303 health sciences, biology, 3. Good health, Sporozoites, Infection and autoimmunity [NCMLS 1], [ INFO.INFO-BT ] Computer Science [cs]/Biotechnology, Plasmodium yoelii, MESH: Cell Nucleus, MESH: Cell Line, Tumor, Plasmodium falciparum, Immunology, Auto-immunity, transplantation and immunotherapy [N4i 4], Microbiology, Host-Parasite Interactions, Tetraspanin 28, MESH: Animals, Genetically Modified, 03 medical and health sciences, Species Specificity, Antigen, Antigens, CD, Cell Line, Tumor, Virology, MESH : Mice, parasitic diseases, MESH : Antigens, CD, Animals, Humans, MESH : Species Specificity, MESH: Species Specificity, MESH: Mice, 030304 developmental biology, Cell Nucleus, MESH: Humans, MESH : Cell Line, Tumor, 030306 microbiology, MESH : Humans, fungi, Poverty-related infectious diseases [N4i 3], [ SDV.BIO ] Life Sciences [q-bio]/Biotechnology, MESH : Plasmodium yoelii, MESH : Host-Parasite Relations, biology.organism_classification, [SDV.BIO] Life Sciences [q-bio]/Biotechnology, [INFO.INFO-BT] Computer Science [cs]/Biotechnology, Cell culture, MESH : Animals, Microbial pathogenesis and host defense [UMCN 4.1], Immunity, infection and tissue repair [NCMLS 1], CD81
Abstract

Contains fulltext : 49897.pdf (Publisher’s version ) (Closed access) Plasmodium sporozoites can enter host cells by two distinct pathways, either through disruption of the plasma membrane followed by parasite transmigration through cells, or by formation of a parasitophorous vacuole (PV) where the parasite further differentiates into a replicative exo-erythrocytic form (EEF). We now provide evidence that following invasion without PV formation, transmigrating Plasmodium falciparum and Plasmodium yoelii sporozoites can partially develop into EEFs inside hepatocarcinoma cell nuclei. We also found that rodent P. yoelii sporozoites can infect both mouse and human hepatocytes, while human P. falciparum sporozoites infect human but not mouse hepatocytes. We have previously reported that the host tetraspanin CD81 is required for PV formation by P. falciparum and P. yoelii sporozoites. Here we show that expression of human CD81 in CD81-knockout mouse hepatocytes is sufficient to confer susceptibility to P. yoelii but not P. falciparum sporozoite infection, showing that the narrow P. falciparum host tropism does not rely on CD81 only. Also, expression of CD81 in a human hepatocarcinoma cell line is sufficient to promote the formation of a PV by P. yoelii but not P. falciparum sporozoites. These results highlight critical differences between P. yoelii and P. falciparum sporozoite infection, and suggest that in addition to CD81, other molecules are specifically required for PV formation during infection by the human malaria parasite.

Published
2006
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7. A P-insertion screen identifying novel X-linked essential genes in Drosophila

Author

Geneviève Gonzy-Treboul, Marc Haenlin, Jean S. Deutsch, David L. Cribbs, Frédérique Peronnet, Pierre Ferrer, Stéphane Noselli, Claude Ardourel, Alain Vincent, Henri-Marc Bourbon, Marie-Francoise Alin, Corinne Benassayag, Jean-Antoine Lepesant, Centre de biologie du développement (CBD), Centre National de la Recherche Scientifique (CNRS)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, Institut Jacques Monod (IJM (UMR_7592)), Centre National de la Recherche Scientifique (CNRS)-Université Paris Diderot - Paris 7 (UPD7), Laboratoire de Biologie du Développement (LBD), Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut de Biologie Paris Seine (IBPS), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Génomique et épigénétique des pathologies placentaires (Inserm U709), Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM), Biologie moléculaire et génie génétique (IGBMC), Institut National de la Santé et de la Recherche Médicale (INSERM), Laboratoire Bordelais de Recherche en Informatique (LaBRI), Centre National de la Recherche Scientifique (CNRS)-École Nationale Supérieure d'Électronique, Informatique et Radiocommunications de Bordeaux (ENSEIRB)-Université Sciences et Technologies - Bordeaux 1-Université Bordeaux Segalen - Bordeaux 2, Institut de Génomique Fonctionnelle (IGF), Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Montpellier 2 - Sciences et Techniques (UM2)-Centre National de la Recherche Scientifique (CNRS), Anatomopathologie Haut-Lévêque (ANATOMOPATHOLOGIE), CHU, Clinical Neurology Oxford (CLINICAL NEUROLOGY), Hospital, Departement /u661 : Endocrinologie, Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de synthèse organique (DCSO), École polytechnique (X)-Centre National de la Recherche Scientifique (CNRS), FLAveur, VIsion et Comportement du consommateur (FLAVIC), Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-Etablissement National d'Enseignement Supérieur Agronomique de Dijon (ENESAD), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre de Biologie Intégrative (CBI), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Université Paris Diderot - Paris 7 (UPD7)-Centre National de la Recherche Scientifique (CNRS), Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Biologie structurale, Université de Bordeaux (UB)-Centre National de la Recherche Scientifique (CNRS)-École Nationale Supérieure d'Électronique, Informatique et Radiocommunications de Bordeaux (ENSEIRB), École polytechnique (X)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Institut National de la Recherche Agronomique (INRA)-Etablissement National d'Enseignement Supérieur Agronomique de Dijon (ENESAD)-Université de Bourgogne (UB), Université de Toulouse (UT)-Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS)-Centre de Biologie Intégrative (CBI), Université de Toulouse (UT)-Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Université de Bordeaux (UB)-École Nationale Supérieure d'Électronique, Informatique et Radiocommunications de Bordeaux (ENSEIRB)-Centre National de la Recherche Scientifique (CNRS), Etablissement National d'Enseignement Supérieur Agronomique de Dijon (ENESAD)-Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris Descartes - Paris 5 (UPD5), Centre de biologie du développement ( CBD ), Université Toulouse III - Paul Sabatier ( UPS ), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique ( CNRS ), Institut Jacques Monod ( IJM ), Université Paris Diderot - Paris 7 ( UPD7 ) -Centre National de la Recherche Scientifique ( CNRS ), Laboratoire de Biologie du Développement ( LBD ), Centre National de la Recherche Scientifique ( CNRS ) -Université Pierre et Marie Curie - Paris 6 ( UPMC ), Génomique et épigénétique des pathologies placentaires ( Inserm U709 ), Université Paris Descartes - Paris 5 ( UPD5 ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ), Biologie moléculaire et génie génétique ( IGBMC ), Institut National de la Santé et de la Recherche Médicale ( INSERM ), Laboratoire Bordelais de Recherche en Informatique ( LaBRI ), Centre National de la Recherche Scientifique ( CNRS ) -École Nationale Supérieure d'Électronique, Informatique et Radiocommunications de Bordeaux (ENSEIRB)-Université Sciences et Technologies - Bordeaux 1-Université Bordeaux Segalen - Bordeaux 2, Institut de Génomique Fonctionnelle ( IGF ), Centre National de la Recherche Scientifique ( CNRS ) -Université Montpellier 2 - Sciences et Techniques ( UM2 ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Université Montpellier 1 ( UM1 ) -Université de Montpellier ( UM ), Anatomopathologie Haut-Lévêque ( ANATOMOPATHOLOGIE ), Clinical Neurology Oxford ( CLINICAL NEUROLOGY ), Université Montpellier 1 ( UM1 ) -Université Montpellier 2 - Sciences et Techniques ( UM2 ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Centre National de la Recherche Scientifique ( CNRS ), Laboratoire de synthèse organique ( DCSO ), École polytechnique ( X ) -Centre National de la Recherche Scientifique ( CNRS ), FLAveur, VIsion et Comportement du consommateur ( FLAVIC ), and Etablissement National d'Enseignement Supérieur Agronomique de Dijon ( ENESAD ) -Institut National de la Recherche Agronomique ( INRA ) -Université de Bourgogne ( UB )

Subjects
Male, Embryology, Genetic Linkage, MESH : Animals, Genetically Modified, Genes, Insect, MESH: Genes, Insect, medicine.disease_cause, Lethal, Genome, Animals, Genetically Modified, 0302 clinical medicine, MESH : Crosses, Genetic, Genes, Reporter, MESH : Drosophila melanogaster, MESH: Gene Expression Regulation, Developmental, MESH : Female, Developmental, MESH: Animals, MESH : Lac Operon, X chromosome, Genetics, 0303 health sciences, Mutation, biology, MESH : Enhancer Elements (Genetics), MESH: Enhancer Elements (Genetics), MESH : Genes, Insect, Linkage (Genetics), Gene Expression Regulation, Developmental, MESH: Lac Operon, MESH : Genes, Reporter, MESH: Crosses, Genetic, 3. Good health, Enhancer Elements, Genetic, Drosophila melanogaster, Lac Operon, Female, MESH : X Chromosome, X Chromosome, MESH : Male, Mutagenesis (molecular biology technique), Genetically Modified, Crosses, MESH: Drosophila melanogaster, MESH: Animals, Genetically Modified, 03 medical and health sciences, Genetic, Insertional, medicine, Animals, Enhancer Elements (Genetics), [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, Enhancer, Gene, [ SDV.BBM ] Life Sciences [q-bio]/Biochemistry, Molecular Biology, Reporter, Crosses, Genetic, 030304 developmental biology, Reporter gene, MESH : Mutagenesis, Insertional, MESH: X Chromosome, MESH: Genes, Reporter, biology.organism_classification, MESH: Male, Mutagenesis, Insertional, Gene Expression Regulation, Genes, MESH: Mutagenesis, Insertional, MESH : Genes, Lethal, Mutagenesis, Genes, Lethal, MESH : Animals, MESH : Gene Expression Regulation, Developmental, MESH: Genes, Lethal, Insect, MESH: Linkage (Genetics), MESH: Female, 030217 neurology & neurosurgery, MESH : Linkage (Genetics), Developmental Biology
Abstract

The recent determination and annotation of the entire euchromatic sequence of the Drosophila melanogaster genome predicted the existence of about 13600 different genes (Science 287 (2000) 2185; http://www.fruitfly.org/annot/index.html). In parallel, the Berkeley Drosophila Genome Project (BDGP) has undertaken systematic P-insertion screens, to isolate new lethals and misexpressing lines. To date, however, the genes of the X chromosome have been under-represented in the screens performed. In order both to characterize several X-linked genes of prime interest to our laboratories and contribute to the collection of lethal P-insertions available to the community, we performed a P-insertion mutagenesis of the X chromosome. Using the PlacW and PGawB P-elements as mutagens, we generated two complementary sets of enhancer-trap lines, l(1)(T)PL and l(1)(T)PG, respectively, which both contain a reporter gene whose developmental expression can be monitored when driven by nearby enhancer sequences. We report here the characterization of 260 new insertions, mapping to 133 different genes or predicted CGs. Of these, 83 correspond to genes for which no lethal mutation had yet been reported. For 64 of those, we could confirm that lethality was solely due to the P-element insertion. The primary molecular data, reporter gene expression patterns (observed in embryos, third instar larvae and adult ovaries) and proposed CG assignment for each strain can be accessed and updated on our website at the following address: http://www-cbd.ups-tlse.fr:8080/screen.

Published
2002
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8. Genetic identification of neurons controlling a sexually dimorphic behaviour

Author

Gatti , S., Ferveur , J. F., Martin , J. R., Neurobiologie de l'apprentissage, de la mémoire et de la communication ( NAMC ), Université Paris-Sud - Paris 11 ( UP11 ) -Centre National de la Recherche Scientifique ( CNRS ), Développement et Communication Chimique chez les Insectes ( DCCI ), and Centre National de la Recherche Scientifique ( CNRS ) -Université de Bourgogne ( UB ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement

Subjects
MESH : Recombinant Fusion Proteins, MESH : Transcription Factors, MESH : Male, fungi, MESH : Animals, Genetically Modified, MESH : Sex Characteristics, MESH : Nuclear Proteins, MESH : Neurons, MESH : Nervous System Physiology, MESH : Saccharomyces cerevisiae Proteins, MESH : Locomotion, [ SDV.NEU ] Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC], MESH : Drosophila melanogaster, MESH : Fungal Proteins, MESH : Female, MESH : Animals, MESH : Sexual Behavior, Animal, MESH : Motor Activity
Abstract

In the fruit fly Drosophila melanogaster, locomotor activity is sexually dimorphic: female flies constantly modulate their activity pattern whereas males show a steadier, stereotyped walking pace [1]. Here, we mapped the area of the brain controlling this behavioural dimorphism. Adult male Drosophila expressing a dominant feminising transgene in a small cluster of neurons in the pars intercerebralis exhibited a female-like pattern of locomotor activity. Genetic ablation of these neurons prevented the feminisation of the locomotor activity of transgenic males. The results suggest that this cluster of neurons modulates sex-specific activity, but is not involved in initiating fly locomotion. Nor does it control male courtship behaviour, because feminisation of courtship was not correlated with the feminisation of locomotor activity.

Published
2000
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