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Your search keyword '"Mariette, Lokate"' showing total 3 results
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3 results on '"Mariette, Lokate"'

1. Detection of Legionella Anisa in Water from Hospital Dental Chair Units and Molecular Characterization by Whole-Genome Sequencing

Author

Giuseppe Fleres, Natacha Couto, Mariette Lokate, Luc W. M. van der Sluis, Christophe Ginevra, Sophie Jarraud, Ruud H. Deurenberg, John W. Rossen, Silvia García-Cobos, and Alex W. Friedrich

Subjects
Legionella spp., whole-genome sequencing (WGS), core-genome multi-locus sequence typing (cgMLST), whole-genome multi-locus sequence typing (wgMLST), next-generation sequencing (NGS), Biology (General), QH301-705.5
Abstract

This study aims to assess contamination with Legionella spp. in water from dental chair units (DCUs) of a hospital dental ward and to perform its molecular characterization by whole-genome sequencing (WGS). We collect eight water samples (250 mL) from four DCUs (sink and water-syringe). Samples are tested for the presence of Legionella spp. (CFUs/mL) by culturing according to the Nederland Norm (NEN) 6265. Three DCUs are found positive for Legionella anisa, and four isolates are cultured (sink n = 2, water-syringe n = 1; two isolates from the same chair) with 1 × 102 CFU/mL. Whole-genome multi-locus sequence typing (wgMLST) results indicate that all strains belong to the same cluster with two to four allele differences. Classical culture combined with WGS allows the identification of a unique clone of L. anisa in several DCUs in the same hospital dental ward. This may indicate a common contamination source in the dental unit waterlines, which was fixed by replacing the chairs and main pipeline of the unit. Our results reveal tap water contamination in direct contact with patients and the usefulness of WGS to investigate bacterial molecular epidemiology.

Published
2018
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2. Detection of

Author

Giuseppe, Fleres, Natacha, Couto, Mariette, Lokate, Luc W M, van der Sluis, Christophe, Ginevra, Sophie, Jarraud, Ruud H, Deurenberg, John W, Rossen, Silvia, García-Cobos, and Alex W, Friedrich

Subjects
Legionella spp, core-genome multi-locus sequence typing (cgMLST), whole-genome multi-locus sequence typing (wgMLST), next-generation sequencing (NGS), whole-genome sequencing (WGS), Article
Abstract

This study aims to assess contamination with Legionella spp. in water from dental chair units (DCUs) of a hospital dental ward and to perform its molecular characterization by whole-genome sequencing (WGS). We collect eight water samples (250 mL) from four DCUs (sink and water-syringe). Samples are tested for the presence of Legionella spp. (CFUs/mL) by culturing according to the Nederland Norm (NEN) 6265. Three DCUs are found positive for Legionella anisa, and four isolates are cultured (sink n = 2, water-syringe n = 1; two isolates from the same chair) with 1 × 102 CFU/mL. Whole-genome multi-locus sequence typing (wgMLST) results indicate that all strains belong to the same cluster with two to four allele differences. Classical culture combined with WGS allows the identification of a unique clone of L. anisa in several DCUs in the same hospital dental ward. This may indicate a common contamination source in the dental unit waterlines, which was fixed by replacing the chairs and main pipeline of the unit. Our results reveal tap water contamination in direct contact with patients and the usefulness of WGS to investigate bacterial molecular epidemiology.

Published
2018

3. Assessing the impact of infection prevention – an incremental cost-benefit analysis

Author

Jan-Willem Dik, Mariette Lokate, Ariane Kirste, Ron Hendrix, Lo-Ten-Foe, J. R., Maarten Postma, Bhanu Sinha, Alex Friedrich, Microbes in Health and Disease (MHD), Methods in Medicines evaluation & Outcomes research (M2O), PharmacoTherapy, -Epidemiology and -Economics, Critical care, Anesthesiology, Peri-operative and Emergency medicine (CAPE), Real World Studies in PharmacoEpidemiology, -Genetics, -Economics and -Therapy (PEGET), and Value, Affordability and Sustainability (VALUE)

Subjects
Cost-Benefit Analysis, ComputingMethodologies_DOCUMENTANDTEXTPROCESSING, NOSOCOMIAL OUTBREAKS, infection prevention, GeneralLiterature_REFERENCE(e.g.,dictionaries,encyclopedias,glossaries)

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