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Background: The dysregulation of microRNAs (miRNAs) has been detected in patients with gastric cancer (GC), which inspired the use of miRNAs as a novel biomarker for GC. In this study, we investigated the previously reported miRNA dysfunction in cancer tissues as a potential plasma biomarker for GC using quantitative reverse transcriptase polymerase chain reaction (RT-PCR). Methods: The published miRNA abnormalities were searched in the microRNA Cancer Association Database. Plasma samples were collected from patients with GC (n = 26) and controls (n = 17). The sensitivity and specificity of polyadenylation RT-PCR (PA-RT) and stem-loop RT-PCR (SL-RT) were compared. Statistical comparisons between patients with GC and controls were performed to identify miRNA biomarkers, and correlation analyses between the threshold cycle (Ct) values of miRNAs and various blood biochemical parameters were performed to elucidate the confounding factors. Results: mir-17, mir-21, mir-31, mir-99b, mir-222, and U6 were selected. PA-RT showed greater sensitivity and lower specificity than SL-RT (PA-RT vs. SL-RT, mean Ct: 19.6 vs. 29.2; coefficient of variation: 0.42 vs. 0.10). Adopting SL-RT owing to its higher specificity, only mir-222 was significantly upregulated in patients with GC (GC vs. control, miRNA expression: 15.4 vs. 5.27, p = 0.0098). Regarding the correlation between blood biochemical parameters and cells with miRNA expression, mir-31 and mir-99b were correlated with blood urea nitrogen, mir-17, mir-21, and mir-99b were negatively correlated with platelets, and mir-21 was correlated with neutrophils. No obvious correlations were noted between mir-222 expression and blood parameters. Receiver operating characteristic (ROC) curve analysis indicated that mir-222 identified GC patients with a maximum area under the curve (0.73, 95% confidence interval 0.57–0.89). Conclusions: Plasma mir-222 was confirmed to be dysregulated in patients with GC, irrespective of blood biochemical parameters. [ABSTRACT FROM AUTHOR]

Not much is known about the changes in bone density due to fish reproductive migration. We used micro-CT and inferential statistics to determine whether the relative bone density in the skulls of adult Three-lips fish, that seasonally upstream migrated to a Lake Biwa tributary, changed across their known reproductive season. The relative bone density significantly decreased as standard length and condition factor (K) increased in both sexes. This negative relationship is likely due to age and hormonal effects in the fish. Results from the bone density analysis also revealed that male Three-lips fish had potentially lower relative bone density (although not significantly different) than females during peak reproductive migration, i.e., July to August. On average, male Three-lips fish are larger in length and weight than females, and in many species, females prefer larger males to smaller males, viewing their size as an indicator of genetic fitness and their ability to provide protection. Resources in the skulls of Three-lips males may be distributed in such a way that increases reproductive success, i.e., size at the expense of quality. In addition, individuals with slightly less dense bones, particularly males, appeared later than those with denser bones during the peak of the reproductive season. The high energy demands involved with aggression in males, often requires resource mobilization from various tissue compartments and could explain the slightly lower density in the latter half of the peak migration. Furthermore, Three-lips individuals that migrated earlier and later during the reproductive season may have more energy reserves than those that had been in the river for some time, hence the variable bone density between individuals. This study serves as a foundation for future studies on the effects of migration, changes in physiology and age on bone density analysis of Three-lips fish and other species in various ecosystems. [ABSTRACT FROM AUTHOR]

The changing climate makes it more difficult to manage water resources and food production sustainably. Various adaptation measures have been proposed to moderate the negative impacts of climate change; however, implementation of these measures may be hampered by other factors even if the benefits are acknowledged—a situation termed "soft adaptation limits" by the IPCC. We hypothesized that societal rules can be a potential barrier to adaptive action if they are too fixed, because such rules have coevolved with the interactions between the past climate and human activities. To test this hypothesis, we present a framework based on the assumption that associated societal rules of Japan's matured irrigation systems are potential barriers to adaptation. The framework consisted of two process‐based models, one to evaluate the water deficit risk and one to evaluate the benefits of optimizing rice yield and quality. We applied each model to an experiment in which we shifted the current transplantation date by 1 week for up to 5 weeks before and after the current date under the historical (1981–2000) and RCP 2.6 and 8.5 (2011–2030 and 2031–2050) scenarios. We revealed two contrasting development pathways in the study watershed. Soft adaptation limits imposed by water availability will occur by 2030 if farmers optimize for quality, whereas mutual benefits to farmers and river administrators will be achieved if farmers seek yield. We argue that more participatory research with stakeholder engagement, as well as policy discussions about these possible developments, is needed to ensure successful adaptation. Key Points: We present a framework for examining whether societal rules of Japan's matured irrigation systems are potential barriers to adaptationWe show that potential barriers to adaptation imposed by water availability will occur if farmers optimize for rice qualityWe argue that more participatory research with stakeholder engagement is needed to ensure successful adaptation [ABSTRACT FROM AUTHOR]

The Hanford Site in Washington State is the most contaminated area in the United States and is one of the biggest decontamination efforts in the world. During the Cold War, the area was shrouded by secrecy, including denials of any dangers to the environment, workers, and local communities downwind of the site. Efforts to regenerate neighboring areas are ongoing, including establishing the area as a national park and national monument, investing in local communities, plans to re-establish a town, encouraging viticulture and other agricultural activities, and encouraging the return of spawning salmon to the Columbia River. Rising costs and delays have hampered efforts in the clean-up process. Through an online survey, this study examines the local people's attitudes toward these various efforts at urban and agricultural regeneration, their attitude toward information from the authorities, and budgeting for decontamination. It was found that there is widespread support for the establishment of park status for the site and that organic certification of produce has had a positive impact on purchasing intentions. However, there remains a degree of skepticism about the information about the decontamination process, and support for budgeting plans falls largely along partisan lines. [ABSTRACT FROM AUTHOR]