32 results on '"Medica D"'
Search Results
2. Role of Lipids in the Transmission of the Infective Stage (L3) of Strongylus vulgaris (Nematoda: Strongylida)
- Author
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Medica, D. L. and Sukhdeo, M. V. K.
- Published
- 1997
- Full Text
- View/download PDF
3. Loss of Nephrin Expression in Glomeruli of Kidney‐Transplanted Patients Under m‐TOR Inhibitor Therapy
- Author
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Biancone, L., Bussolati, B., Mazzucco, G., Barreca, A., Gallo, E., Rossetti, M., Messina, M, Nuschak, B., Fop, F., Medica, D., Cantaluppi, V., Camussi, G., and Segoloni, G.P
- Published
- 2010
- Full Text
- View/download PDF
4. Extracranial Organ Dysfunction Due to Systemic Inflammation in Critically Ill Patients With Traumatic Brain Injury: A Potential Cause of Subclinical Acute Renal Damage in Kidney Transplantation Donors.: Abstract# B814
- Author
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Cantaluppi, V., Civiletti, F., Medica, D., Mazzeo, A., Assenzio, B., Mastromauro, I., Deambrosis, I., Giaretta, F., Fanelli, V., and Mascia, L.
- Published
- 2014
5. Plasma NGAL Is an Early Biomarker of Graft Function, Calcineurin Inhibitor Nephrotoxicity and Tubular Regeneration in Kidney Transplantation from Extended Criteria Donors.: Abstract# 1147 Poster Board #-Session: P14-III
- Author
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Cantaluppi, V., Tamagnone, M., Dellepiane, S., Manzione, A. M., Messina, M., Ranghino, A., Medica, D., Biancone, L., Camussi, G., and Segoloni, G. P.
- Published
- 2012
6. Microvesicles Derived from Endothelial Progenitor Cells Protect from Antibody- and Complement-Mediated Endothelial Injury through the Horizontal Transfer of Specific mRNAs and microRNAs.: Abstract# 78
- Author
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Cantaluppi, V., Delena, M., Medica, D., Figliolini, F., Beltramo, S., Tognarelli, G., Biancone, L., Segoloni, G. P., and Camussi, G.
- Published
- 2012
7. EXPERIMENTAL KIDNEY INJURY MECHANISMS
- Author
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Oliveira-Salles, E. B., primary, Maquigussa, E., additional, Semedo, P., additional, Pereira, L. G., additional, Ferreira, V. M., additional, Camara, N. O., additional, Bergamaschi, C. T., additional, Campos, R. R., additional, Boim, M. A., additional, Cantaluppi, V., additional, Medica, D., additional, Figliolini, F., additional, Quercia, A. D., additional, Dellepiane, S., additional, Virzi', G. M., additional, Ronco, C., additional, Tetta, C., additional, Biancone, L., additional, Camussi, G., additional, Wada, Y., additional, Iyoda, M., additional, Matsumoto, K., additional, Shindo-Hirai, Y., additional, Kuno, Y., additional, Suzuki, T., additional, Yamamoto, Y., additional, Saito, T., additional, Iseri, K., additional, Shibata, T., additional, Albertoni, G., additional, Reis, L. A., additional, Schor, N., additional, Fonseca, C. D., additional, Watanabe, M., additional, Mendonca, M. H., additional, Fernandes, S. M., additional, and Vattimo, M. D. F. F., additional
- Published
- 2014
- Full Text
- View/download PDF
8. TRANSPLANTATION CLINICAL 1
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Schachtner, T., primary, Reinke, P., additional, Dorje, C., additional, Mjoen, G., additional, Midtvedt, K., additional, Strom, E. H., additional, Oyen, O., additional, Jenssen, T., additional, Reisaeter, A. V., additional, Smedbraaten, Y. V., additional, Sagedal, S., additional, Fagerland, M. W., additional, Hartmann, A., additional, Thiel, S., additional, Zulkarnaev, A., additional, Vatazin, A., additional, Vincenti, F., additional, Harel, E., additional, Kantor, A., additional, Thurison, T., additional, Hoyer-Hansen, G., additional, Craik, C., additional, Kute, V. B., additional, Shah, P. S., additional, Vanikar, A. V., additional, Modi, P. R., additional, Shah, P. R., additional, Gumber, M. R., additional, Patel, H. V., additional, Engineer, D. P., additional, Shah, V. R., additional, Rizvi, J., additional, Trivedi, H. L., additional, Malheiro, J., additional, Dias, L., additional, Martins, L. S., additional, Fonseca, I., additional, Pedroso, S., additional, Almeida, M., additional, Castro-Henriques, A., additional, Cabrita, A., additional, Costa, C., additional, Ritta, M., additional, Sinesi, F., additional, Sidoti, F., additional, Mantovani, S., additional, Di Nauta, A., additional, Messina, M., additional, Cavallo, R., additional, Verflova, A., additional, Svobodova, E., additional, Slatinska, J., additional, Slavcev, A., additional, Pokorna, E., additional, Viklicky, O., additional, Yagan, J., additional, Chandraker, A., additional, Diena, D., additional, Tognarelli, G., additional, Ranghino, A., additional, Bussolino, S., additional, Fop, F., additional, Segoloni, G. P., additional, Biancone, L., additional, Leone, F., additional, Mauro, M. V., additional, Gigliotti, P., additional, Lofaro, D., additional, Greco, F., additional, Perugini, D., additional, Papalia, T., additional, Perri, A., additional, Vizza, D., additional, Giraldi, C., additional, Bonofilgio, R., additional, Luis-Lima, S., additional, Marrero, D., additional, Gonzalez-Rinne, A., additional, Torres, A., additional, Salido, E., additional, Jimenez-Sosa, A., additional, Aldea-Perona, A., additional, Gonzalez-Posada, J. M., additional, Perez-Tamajon, L., additional, Rodriguez-Hernandez, A., additional, Negrin-Mena, N., additional, Porrini, E., additional, Pihlstrom, H., additional, Dahle, D. O., additional, Holdaas, H., additional, Von Der Lippe, N., additional, Waldum, B., additional, Brekke, F., additional, Amro, A., additional, Os, I., additional, Klin, P., additional, Sanabria, H., additional, Bridoux, P., additional, De Francesco, J., additional, Fortunato, R. M., additional, Raffaele, P., additional, Kong, J., additional, Son, S. H., additional, Kwon, H. Y., additional, Whang, E. J., additional, Choi, W. Y., additional, Yoon, C. S., additional, Thanaraj, V., additional, Theakstone, A., additional, Stopper, K., additional, Ferraro, A., additional, Bhattacharjya, S., additional, Devonald, M., additional, Williams, A., additional, Mella, A., additional, Gallo, E., additional, Di Vico, M. C., additional, Pagani, F., additional, Gai, M., additional, Cho, H. J., additional, Nho, K. W., additional, Park, S.-K., additional, Kim, S. B., additional, Yoshida, K., additional, Ishii, D., additional, Ohyama, T., additional, Kohguchi, D., additional, Takeuchi, Y., additional, Varga, A., additional, Sandor, B., additional, Kalmar-Nagy, K., additional, Toth, A., additional, Toth, K., additional, Szakaly, P., additional, Kildushevsky, A., additional, Fedulkina, V., additional, Kantaria, R., additional, Staeck, O., additional, Halleck, F., additional, Rissling, O., additional, Naik, M., additional, Neumayer, H.-H., additional, Budde, K., additional, Khadzhynov, D., additional, Bhadauria, D., additional, Kaul, A., additional, Prasad, N., additional, Sharma, R. K., additional, Sezer, S., additional, Bal, Z., additional, Erkmen Uyar, M., additional, Guliyev, O., additional, Erdemir, B., additional, Colak, T., additional, Ozdemir, N., additional, Haberal, M., additional, Caliskan, Y., additional, Yazici, H., additional, Artan, A. S., additional, Oto, O. A., additional, Aysuna, N., additional, Bozfakioglu, S., additional, Turkmen, A., additional, Yildiz, A., additional, Sever, M. S., additional, Yagisawa, T., additional, Nukui, A., additional, Kimura, T., additional, Nannmoku, K., additional, Kurosawa, A., additional, Sakuma, Y., additional, Miki, A., additional, Damiano, F., additional, Ligabue, G., additional, De Biasi, S., additional, Granito, M., additional, Cossarizza, A., additional, Cappelli, G., additional, Henriques, A. C., additional, Davide, J., additional, Von During, M. E., additional, Jenssen, T. G., additional, Bollerslev, J., additional, Godang, K., additional, Asberg, A., additional, Bachelet, T., additional, Martinez, C., additional, Bello, A., additional, Kejji, S., additional, Couzi, L., additional, Guidicelli, G., additional, Lepreux, S., additional, Visentin, J., additional, Congy-Jolivet, N., additional, Rostaing, L., additional, Taupin, J.-L., additional, Kamar, N., additional, Merville, P., additional, Ozdemir, H., additional, Yildirim, S., additional, Tutal, E., additional, Sayin, B., additional, Ozdemir Acar, N., additional, Banasik, M., additional, Boratynska, M., additional, Koscielska-Kasprzak, K., additional, Kaminska, D., additional, Bartoszek, D., additional, Mazanowska, O., additional, Krajewska, M., additional, Zmonarski, S., additional, Chudoba, P., additional, Dawiskiba, T., additional, Protasiewicz, M., additional, Halon, A., additional, Sas, A., additional, Kaminska, M., additional, Klinger, M., additional, Stefanovic, N., additional, Cvetkovic, T., additional, Velickovic - Radovanovic, R., additional, Jevtovic - Stoimenov, T., additional, Vlahovic, P., additional, Rungta, R., additional, Das, P., additional, Ray, D. S., additional, Gupta, S., additional, Kolonko, A., additional, Szotowska, M., additional, Kuczera, P., additional, Chudek, J., additional, Wiecek, A., additional, Sikora-Grabka, E., additional, Adamczak, M., additional, Madej, P., additional, Amanova, A., additional, Kendi Celebi, Z., additional, Bakar, F., additional, Caglayan, M. G., additional, Keven, K., additional, Massimetti, C., additional, Imperato, G., additional, Zampi, G., additional, De Vincenzi, A., additional, Fabbri, G. D. D., additional, Brescia, F., additional, Feriozzi, S., additional, Filipov, J. J., additional, Zlatkov, B. K., additional, Dimitrov, E. P., additional, Svinarov, D. A., additional, Poesen, R., additional, De Vusser, K., additional, Evenepoel, P., additional, Kuypers, D., additional, Naesens, M., additional, Meijers, B., additional, Kocak, H., additional, Yilmaz, V. T., additional, Yilmaz, F., additional, Uslu, H. B., additional, Aliosmanoglu, I., additional, Ermis, H., additional, Dinckan, A., additional, Cetinkaya, R., additional, Ersoy, F. F., additional, Suleymanlar, G., additional, Oliveira, J.-C., additional, Santos, J., additional, Lobato, L., additional, Mendonca, D., additional, Watarai, Y., additional, Yamamoto, T., additional, Tsujita, M., additional, Hiramitsu, T., additional, Goto, N., additional, Narumi, S., additional, Kobayashi, T., additional, Line, P.-D., additional, Housawi, A., additional, House, A., additional, Ng, C., additional, Denesyk, K., additional, Rehman, F., additional, Moist, L., additional, Musetti, C., additional, Battista, M., additional, Izzo, C., additional, Guglielmetti, G., additional, Airoldi, A., additional, Stratta, P., additional, Cena, T., additional, Quaglia, M., additional, Fenoglio, R., additional, Cagna, D., additional, Amoroso, A., additional, Palmisano, A., additional, Degli Antoni, A. M., additional, Vaglio, A., additional, Piotti, G., additional, Cremaschi, E., additional, Buzio, C., additional, Maggiore, U., additional, Lee, M.-C., additional, Hsu, B.-G., additional, Zalamea Jarrin, F., additional, Sanchez Sobrino, B., additional, Lafuente Covarrubias, O., additional, Karsten Alvarez, S., additional, Dominguez Apinaniz, P., additional, Llopez Carratala, R., additional, Portoles Perez, J., additional, Yildirim, T., additional, Yilmaz, R., additional, Turkmen, E., additional, Altindal, M., additional, Arici, M., additional, Altun, B., additional, Erdem, Y., additional, Dounousi, E., additional, Mitsis, M., additional, Naka, K., additional, Pappas, H., additional, Lakkas, L., additional, Harisis, H., additional, Pappas, K., additional, Koutlas, V., additional, Tzalavra, I., additional, Spanos, G., additional, Michalis, L., additional, Siamopoulos, K., additional, Iwabuchi, T., additional, Nanmoku, K., additional, Yasunaru, S., additional, Yoshikawa, M., additional, Kitamura, K., additional, Fuji, H., additional, Fujisawa, M., additional, Nishi, S., additional, Carta, P., additional, Zanazzi, M., additional, Buti, E., additional, Larti, A., additional, Caroti, L., additional, Di Maria, L., additional, Minetti, E. E., additional, Shi, Y., additional, Luo, L., additional, Cai, B., additional, Wang, T., additional, Zou, Y., additional, Wang, L., additional, Kim, Y., additional, Kim, H. S., additional, Choi, B. S., additional, Park, C. W., additional, Yang, C. W., additional, Kim, Y.-S., additional, Chung, B. H., additional, Baek, C. H., additional, Kim, M., additional, Kim, J.-S., additional, Yang, W. S., additional, Han, D. J., additional, Mikolasevic, I., additional, Racki, S., additional, Lukenda, V., additional, Persic, M. P., additional, Colic, M., additional, Devcic, B., additional, Orlic, L., additional, Gurlek Demirci, B., additional, Say N, C. B., additional, Ozdemir Acar, F. N., additional, Vali, S., additional, Ismal, K., additional, Sahay, M., additional, Civiletti, F., additional, Cantaluppi, V., additional, Medica, D., additional, Mazzeo, A. T., additional, Assenzio, B., additional, Mastromauro, I., additional, Deambrosis, I., additional, Giaretta, F., additional, Fanelli, V., additional, Mascia, L., additional, Gkirdis, I., additional, Bechlioulis, A., additional, Evangelou, D., additional, Zarzoulas, F., additional, Kotsia, A., additional, Balafa, O., additional, Tzeltzes, G., additional, Nakas, G., additional, Kalaitzidis, R., additional, Katsouras, C., additional, Uyanik, S., additional, Toprak, S. K., additional, Ilhan, O., additional, Ekmen Uyar, M., additional, Hernandez Vargas, H., additional, Artamendi Larranaga, M., additional, Ramalle Gomara, E., additional, Gil Catalinas, F., additional, Bello Ovalle, A., additional, Pimentel Guzman, G., additional, Coloma Lopez, A., additional, Sierra Carpio, M., additional, Gil Paraiso, A., additional, Dall Anesse, C., additional, Beired Val, I., additional, Huarte Loza, E., additional, Choy, B. Y., additional, Kwan, L., additional, Mok, M., additional, Chan, T. M., additional, Yamakawa, T., additional, Kobayashi, A., additional, Yamamoto, I., additional, Mafune, A., additional, Nakada, Y., additional, Tannno, Y., additional, Tsuboi, N., additional, Yamamoto, H., additional, Yokoyama, K., additional, Ohkido, I., additional, Yokoo, T., additional, Luque, Y., additional, Anglicheau, D., additional, Rabant, M., additional, Clement, R., additional, Kreis, H., additional, Sartorius, A., additional, Noel, L.-H., additional, Timsit, M.-O., additional, Legendre, C., additional, Rancic, N., additional, Vavic, N., additional, Dragojevic-Simic, V., additional, Katic, J., additional, Jacimovic, N., additional, Kovacevic, A., additional, Mikov, M., additional, Veldhuijzen, N. M. H., additional, Rookmaaker, M. B., additional, Van Zuilen, A. D., additional, Nquyen, T. Q., additional, Boer, W. H., additional, Sahtout, W., additional, Ghezaiel, H., additional, Azzebi, A., additional, Ben Abdelkrim, S., additional, Guedri, Y., additional, Mrabet, S., additional, Nouira, S., additional, Ferdaws, S., additional, Amor, S., additional, Belarbia, A., additional, Zellama, D., additional, Mokni, M., additional, Achour, A., additional, Parikova, A., additional, Hanzal, V., additional, Fronek, J., additional, Orandi, B. J., additional, James, N. T., additional, Montgomery, R. A., additional, Desai, N. M., additional, Segev, D. L., additional, Fontana, F., additional, Ballestri, M., additional, and Magistroni, R., additional
- Published
- 2014
- Full Text
- View/download PDF
9. Diabetes - experimental models
- Author
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Blanco-Gozalo, V., primary, Blazquez-Medela, A., additional, Garcia-Sanchez, O., additional, Quiros, Y., additional, Montero, M., additional, Martinez-Salgado, C., additional, Lopez-Hernandez, F., additional, Lopez-Novoa, J., additional, Yao, L., additional, Qing, Z., additional, Hua, X., additional, Min, F., additional, Fei, M., additional, Ning, W., additional, Cantaluppi, V., additional, Figliolini, F., additional, Delena, M., additional, Beltramo, S., additional, Medica, D., additional, Tetta, C., additional, Segoloni, G., additional, Biancone, L., additional, Camussi, G., additional, Cunha, J. S., additional, Ferreira, V. M., additional, Naves, M. A., additional, Boim, M. A., additional, Zitman-Gal, T., additional, Golan, E., additional, Green, J., additional, Pasmanik-Chor, M., additional, Bernheim, J., additional, Benchetrit, S., additional, Riera, M., additional, Clotet, S., additional, Pascual, J., additional, Soler, M., additional, Nakai, K., additional, Fujii, H., additional, Kono, K., additional, Goto, S., additional, Hirata, M., additional, Shinohara, M., additional, Fukagawa, M., additional, Nishi, S., additional, Fan, Q., additional, Du, S., additional, Jiang, Y., additional, Wang, L., additional, Fang, L., additional, Radovits, T., additional, Mozes, M. M., additional, Rosivall, L., additional, Kokeny, G., additional, Aoki, R., additional, Tateoka, R., additional, Sekine, F., additional, Kikuchi, K., additional, Yamashita, Y., additional, Itoh, Y., additional, Cappuccino, L., additional, Garibotto, G., additional, D'Amato, E., additional, Villaggio, B., additional, Gianiorio, F., additional, Mij, M., additional, Viazzi, F., additional, Salvidio, G., additional, Verzola, D., additional, Piwkowska, A., additional, Rogacka, D., additional, Audzeyenka, I., additional, Kasztan, M., additional, Angielski, S., additional, Jankowski, M., additional, Gaber, E. W., additional, El-Attar, H. A., additional, Liu, J., additional, Zhang, W., additional, He, Y., additional, Macsai, E., additional, Takats, Z., additional, Derzbach, L., additional, Korner, A., additional, Vasarhelyi, B., additional, Huang, M. S., additional, Bo, H., additional, Liu, F., additional, Fu, P., additional, Tsotakos, N. E., additional, Tsilibary, E. C., additional, Drossopoulou, G. I., additional, Thawho, N., additional, Farid, N., additional, Peleg, A., additional, Levy, A., additional, Nakhoul, N., additional, Lenghel, A. R., additional, Borza, G., additional, Catoi, C., additional, Bondor, C. I., additional, Muresan, A., additional, Kacso, I. M., additional, Song, J.-S., additional, Song, J.-H., additional, Ahn, S.-H., additional, Choi, B. S., additional, Hong, Y. a., additional, Kim, M. Y., additional, Lim, J. H., additional, Yang, K.-S., additional, Chung, S., additional, Shin, S. J., additional, Kim, H. W., additional, Chang, Y. S., additional, Kim, Y. S., additional, Park, C. W., additional, Takayanagi, K., additional, Hasegawa, H., additional, Shimizu, T., additional, Ikari, A., additional, Noiri, C., additional, Iwashita, T., additional, Tayama, Y., additional, Asakura, J., additional, Anzai, N., additional, Kanozawa, K., additional, Kato, H., additional, Mitarai, T., additional, Huang, M., additional, Ashour, R. H., additional, Fouda, A. E.-M. M., additional, Saad, M. A., additional, El-Banna, F. M., additional, Moustafa, F. A., additional, Fouda, M. I., additional, Sanchez-Nino, M. D., additional, Sanz, A. B., additional, Poveda, J., additional, Saleem, M., additional, Mathieson, P., additional, Ruiz-Ortega, M., additional, Selgas, R., additional, Egido, J., additional, Ortiz, A., additional, Soler, M. J., additional, Rebull, M., additional, Marquez, E., additional, Okazaki, S., additional, Kogure, Y., additional, Sano, T., additional, Hatano, M., additional, Kreft, E., additional, Kowalski, R., additional, Szczepansk-Konkel, M., additional, Liu, X., additional, Yang, G., additional, Osman, N. A., additional, NasrAllah, M. M., additional, Kamal, M. M., additional, Ahmed, A. I., additional, Fekih-Mrissa, N., additional, Mrad, M., additional, Baffoun, A., additional, Sayeh, A., additional, Hmida, J., additional, Gritli, N., additional, Galchinskaya, V., additional, Topchii, I., additional, Semenovykh, P., additional, Yefimova, N., additional, Zheng, D., additional, Hu, D., additional, Li, X., additional, Peng, A. I., additional, Olea-Herrero, N., additional, Arenas, M., additional, Munoz-Moreno, C., additional, Moreno-Gomez-Toledano, R., additional, Gonzalez-Santander, M., additional, Arribas, I., additional, and Bosch, R., additional
- Published
- 2013
- Full Text
- View/download PDF
10. Tubular ischemia and toxicity
- Author
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Cantaluppi, V., primary, Medica, D., additional, Figliolini, F., additional, Gatti, S., additional, Bruno, S., additional, Quercia, A. D., additional, Dellepiane, S., additional, Biancone, L., additional, Tetta, C., additional, Camussi, G., additional, Zhou, L., additional, Dai, X., additional, Feng, M., additional, Huang, X., additional, Fu, P., additional, Lan, H. Y., additional, de Ramon, L., additional, Ripoll, E., additional, Luzardo, L., additional, Merino, A., additional, Bolanos, N., additional, Lloberas, N., additional, Cruzado, J. M., additional, Grinyo, J. M., additional, Torras, J., additional, Kaucsar, T., additional, Revesz, C., additional, Godo, M., additional, Racz, Z., additional, Tarszabo, R., additional, Hamar, P., additional, Banki, N. F., additional, Hosszu, A., additional, Antal, Z., additional, Koszegi, S., additional, Wagner, L., additional, Gellai, R., additional, Lenart, L., additional, Vannay, A., additional, Muller, V., additional, Szabo, A. J., additional, Tulassay, T., additional, and Fekete, A., additional
- Published
- 2013
- Full Text
- View/download PDF
11. Glomerular injury
- Author
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Djudjaj, S., primary, Lue, H., additional, Urzinicok, T., additional, Engel, D., additional, Martin, I. V., additional, Buhl, E. M., additional, Floege, J., additional, Ostendorf, T., additional, Bernhagen, J., additional, Boor, P., additional, Cantaluppi, V., additional, Medica, D., additional, Mannari, C., additional, Figliolini, F., additional, Migliori, M., additional, Panichi, V., additional, Tetta, C., additional, Camussi, G., additional, Schulte, K., additional, Berger, K., additional, Sicking, E. M., additional, Jirak, P., additional, Thevissen, L., additional, Fuss, A., additional, Kriz, W., additional, Smeets, B., additional, Moeller, M. J., additional, Santhosh Kumar, V. R., additional, Kulkarni, O. P., additional, Darisipudi, N. M., additional, Mulay, S. R., additional, Anders, H.-J., additional, Assady, S., additional, Alter, J., additional, Litvak, M., additional, Ilan, N., additional, Vlodavsky, I., additional, and Abassi, Z., additional
- Published
- 2013
- Full Text
- View/download PDF
12. Kidney in sepsis
- Author
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Fortrie, G., primary, de Geus, H. R., additional, Betjes, M. G., additional, van Schaik, R. H., additional, Groeneveld, J., additional, Cantaluppi, V., additional, Figliolini, F., additional, Medica, D., additional, Quercia, A. D., additional, Inguaggiato, P., additional, Pacitti, A., additional, Camussi, G., additional, and Tetta, C., additional
- Published
- 2013
- Full Text
- View/download PDF
13. Experimental pathology
- Author
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Yi Chun, D. X., primary, Alexandre, H., additional, Edith, B., additional, Nacera, O., additional, Julie, P., additional, Chantal, J., additional, Eric, R., additional, Zhang, X., additional, Jin, Y., additional, Miravete, M., additional, Dissard, R., additional, Klein, J., additional, Gonzalez, J., additional, Caubet, C., additional, Pecher, C., additional, Pipy, B., additional, Bascands, J.-L., additional, Mercier-Bonin, M., additional, Schanstra, J., additional, Buffin-Meyer, B., additional, Claire, R., additional, Rigothier, C., additional, Richard, D., additional, Sebastien, L., additional, Moin, S., additional, Chantal, B., additional, Christian, C., additional, Jean, R., additional, Migliori, M., additional, Cantaluppi, V., additional, Mannari, C., additional, Medica, D., additional, Giovannini, L., additional, Panichi, V., additional, Goldwich, A., additional, Alexander, S., additional, Andre, G., additional, Amann, K., additional, Migliorini, A., additional, Sagrinati, C., additional, Angelotti, M. L., additional, Mulay, S. R., additional, Ronconi, E., additional, Peired, A., additional, Romagnani, P., additional, Anders, H.-J., additional, Chiang, W. C., additional, Lai, C. F., additional, Peng, W.-H., additional, Wu, C. F., additional, Chang, F.-C., additional, Chen, Y.-T., additional, Lin, S.-L., additional, Chen, Y. M., additional, Wu, K. D., additional, Lu, K.-S., additional, Tsai, T. J., additional, Virgine, O., additional, Qing Feng, F., additional, Zhang, S.-Y., additional, Dominique, D., additional, Vincent, A., additional, Marina, C., additional, Philippe, L., additional, Georges, G., additional, Pawlak, A., additional, Sahali, D., additional, Matsumoto, S., additional, Kiyomoto, H., additional, Ichimura, A., additional, Dan, T., additional, Nakamichi, T., additional, Tsujita, T., additional, Akahori, K., additional, Ito, S., additional, Miyata, T., additional, Xie, S., additional, Zhang, B., additional, Shi, W., additional, Yang, Y., additional, Nagasu, H., additional, Satoh, M., additional, Kidokoro, K., additional, Nishi, Y., additional, Ihoriya, C., additional, Kadoya, H., additional, Sasaki, T., additional, Kashihara, N., additional, Wu, C.-F., additional, Chou, Y.-H., additional, Duffield, J., additional, Rocca, C., additional, Gregorini, M., additional, Corradetti, V., additional, Valsania, T., additional, Bedino, G., additional, Bosio, F., additional, Pattonieri, E. F., additional, Esposito, P., additional, Sepe, V., additional, Libetta, C., additional, Rampino, T., additional, Dal Canton, A., additional, Omori, H., additional, Kawada, N., additional, Inoue, K., additional, Ueda, Y., additional, Yamamoto, R., additional, Matsui, I., additional, Kaimori, J., additional, Takabatake, Y., additional, Moriyama, T., additional, Isaka, Y., additional, Rakugi, H., additional, Wasilewska, A., additional, Taranta-Janusz, K., additional, Deebek, W., additional, Kuroczycka-Saniutycz, E., additional, Lee, A. S., additional, Lee, J. E., additional, Jung, Y. J., additional, Kang, K. P., additional, Lee, S., additional, Kim, W., additional, Arfian, N., additional, Emoto, N., additional, Yagi, K., additional, Nakayama, K., additional, Hartopo, A. B., additional, Nugrahaningsih, D. A., additional, Yanagisawa, M., additional, Hirata, K.-I., additional, Munoz-Felix, J. M., additional, Lopez-Novoa, J. M., additional, Martinez-Salgado, C., additional, Oujo, B., additional, Arevalo, M., additional, Bernabeu, C., additional, Perez-Barriocanal, F., additional, Jesper, K., additional, Nathalie, V., additional, Pierre, G., additional, Yi Chun, D. X., additional, Iyoda, M., additional, Shibata, T., additional, Matsumoto, K., additional, Shindo-Hirai, Y., additional, Kuno, Y., additional, Wada, Y., additional, Akizawa, T., additional, Schwartz, I., additional, Schwartz, D., additional, Prot Bertoye, C., additional, Terryn, S., additional, Claver, J., additional, Beghdadi, W. B., additional, Monteiro, R., additional, Blank, U., additional, Devuyst, O., additional, Daugas, E., additional, Van Beneden, K., additional, Geers, C., additional, Pauwels, M., additional, Mannaerts, I., additional, Van den Branden, C., additional, Van Grunsven, L. A., additional, Seckin, I., additional, Pekpak, M., additional, Uzunalan, M., additional, Uruluer, B., additional, Kokturk, S., additional, Ozturk, Z., additional, Sonmez, H., additional, Yaprak, E., additional, Furuno, Y., additional, Tsutsui, M., additional, Morishita, T., additional, Shimokawa, H., additional, Otsuji, Y., additional, Yanagihara, N., additional, Kabashima, N., additional, Ryota, S., additional, Kanegae, K., additional, Miyamoto, T., additional, Nakamata, J., additional, Ishimatsu, N., additional, Tamura, M., additional, Nakagawa, T., additional, Ichikawa, K., additional, Miyamoto, M., additional, Takabayashi, D., additional, Yamazaki, H., additional, Kakeshita, K., additional, Koike, T., additional, Kagitani, S., additional, Tomoda, F., additional, Hamashima, T., additional, Ishii, Y., additional, Inoue, H., additional, Sasahara, M., additional, El Machhour, F., additional, Kerroch, M., additional, Mesnard, L., additional, Chatziantoniou, C., additional, Dussaule, J.-C., additional, Inui, K., additional, Sasai, F., additional, Maruta, Y., additional, Nishiwaki, H., additional, Kawashima, E., additional, Inoue, Y., additional, Yoshimura, A., additional, Musacchio, E., additional, Priante, G., additional, Valvason, C., additional, Sartori, L., additional, Baggio, B., additional, Kim, J. H., additional, Gross, O., additional, Diana, R., additional, Gry, D. H., additional, Asimal, B., additional, Johanna, T., additional, Imke, S.-E., additional, Lydia, W., additional, Gerhard-Anton, M., additional, Hassan, D., additional, Cano, J. L., additional, Griera, M., additional, Olmos, G., additional, Martin, P., additional, Cortes, M. A., additional, Lopez-Ongil, S., additional, Rodriguez-Puyol, D., additional, DE Frutos, S., additional, Gonzalez, M., additional, Luengo, A., additional, Rodriguez-Puyol, M., additional, Calleros, L., additional, Lupica, R., additional, Lacquaniti, A., additional, Donato, V., additional, Maggio, R., additional, Mastroeni, C., additional, Lucisano, S., additional, Cernaro, V., additional, Fazio, M. R., additional, Quartarone, A., additional, Buemi, M., additional, Kacik, M., additional, Goedicke, S., additional, Eggert, H., additional, Hoyer, J. D., additional, Wurm, S., additional, Steege, A., additional, Banas, M., additional, Kurtz, A., additional, Banas, B., additional, Lasagni, L., additional, Lazzeri, E., additional, Romoli, S., additional, Schaefer, I., additional, Teng, B., additional, Worthmann, K., additional, Haller, H., additional, Schiffer, M., additional, Prattichizzo, C., additional, Netti, G. S., additional, Rocchetti, M. T., additional, Cormio, L., additional, Carrieri, G., additional, Stallone, G., additional, Grandaliano, G., additional, Ranieri, E., additional, Gesualdo, L., additional, Kucher, A., additional, Smirnov, A., additional, Parastayeva, M., additional, Beresneva, O., additional, Kayukov, I., additional, Zubina, I., additional, Ivanova, G., additional, Abed, A., additional, Schlekenbach, L., additional, Foglia, B., additional, Kwak, B., additional, Chadjichristos, C., additional, Queisser, N., additional, Schupp, N., additional, Brand, S., additional, Himer, L., additional, Szebeni, B., additional, Sziksz, E., additional, Saijo, S., additional, Kis, E., additional, Prokai, A., additional, Banki, N. F., additional, Fekete, A., additional, Tulassay, T., additional, Vannay, A., additional, Hegner, B., additional, Schaub, T., additional, Lange, C., additional, Dragun, D., additional, Klinkhammer, B. M., additional, Rafael, K., additional, Monika, M., additional, Anna, M., additional, Van Roeyen, C., additional, Boor, P., additional, Eva Bettina, B., additional, Simon, O., additional, Esther, S., additional, Floege, J., additional, Kunter, U., additional, Janke, D., additional, Jankowski, J., additional, Hayashi, M., additional, Takamatsu, I., additional, Horimai, C., additional, Yoshida, T., additional, Seno DI Marco, G., additional, Koenig, M., additional, Stock, C., additional, Reiermann, S., additional, Amler, S., additional, Koehler, G., additional, Fobker, M., additional, Buck, F., additional, Pavenstaedt, H., additional, Lang, D., additional, Brand, M., additional, Plotnikov, E., additional, Morosanova, M., additional, Pevzner, I., additional, Zorova, L., additional, Pulkova, N., additional, Zorov, D., additional, Wornle, M., additional, Ribeiro, A., additional, Belling, F., additional, Merkle, M., additional, Nakazawa, D., additional, Nishio, S., additional, Shibasaki, S., additional, Tomaru, U., additional, Akihiro, I., additional, Kobayashi, I., additional, Imanishi, Y., additional, Kurajoh, M., additional, Nagata, Y., additional, Yamagata, M., additional, Emoto, M., additional, Michigami, T., additional, Ishimura, E., additional, Inaba, M., additional, Wu, C.-C., additional, Lu, K.-C., additional, Chen, J.-S., additional, Chu, P., additional, Lin, Y.-F., additional, Eller, K., additional, Schroll, A., additional, Kirsch, A., additional, Huber, J., additional, Weiss, G., additional, Theurl, I., additional, Rosenkranz, A. R., additional, Zawada, A., additional, Rogacev, K., additional, Achenbach, M., additional, Fliser, D., additional, Held, G., additional, Heine, G. H., additional, Miyamoto, Y., additional, Iwao, Y., additional, Watanabe, H., additional, Kadowaki, D., additional, Ishima, Y., additional, Chuang, V. T. G., additional, Sato, K., additional, Otagiri, M., additional, Maruyama, T., additional, Iwatani, H., additional, Honda, D., additional, Noguchi, T., additional, Tanaka, M., additional, Tanaka, H., additional, Fukagawa, M., additional, Pircher, J., additional, Koppel, S., additional, Mannell, H., additional, Krotz, F., additional, Virzi, G. M., additional, Bolin, C., additional, Cruz, D., additional, Scalzotto, E., additional, De Cal, M., additional, Vescovo, G., additional, Ronco, C., additional, Grobmayr, R., additional, Lech, M., additional, Ryu, M., additional, Aoshima, Y., additional, Mizobuchi, M., additional, Ogata, H., additional, Kumata, C., additional, Nakazawa, A., additional, Kondo, F., additional, Ono, N., additional, Koiwa, F., additional, Kinugasa, E., additional, Freisinger, W., additional, Lale, N., additional, Lampert, A., additional, Ditting, T., additional, Heinlein, S., additional, Schmieder, R. E., additional, Veelken, R., additional, Nave, H., additional, Perthel, R., additional, Suntharalingam, M., additional, Bode-Boger, S., additional, Beutel, G., additional, Kielstein, J., additional, Rodrigues-Diez, R., additional, Rayego-Mateos, S., additional, Lavoz, C., additional, Stark Aroeira, L. G., additional, Orejudo, M., additional, Alique, M., additional, Ortiz, A., additional, Egido, J., additional, Ruiz-Ortega, M., additional, Oskar, W., additional, Rusan, C., additional, Padberg, J.-S., additional, Wiesinger, A., additional, Reuter, S., additional, Grabner, A., additional, Kentrup, D., additional, Lukasz, A., additional, Oberleithner, H., additional, Pavenstadt, H., additional, Kumpers, P., additional, Eberhardt, H. U., additional, Skerka, C., additional, Chen, Q., additional, Hallstroem, T., additional, Hartmann, A., additional, Kemper, M. J., additional, Zipfel, P. F., additional, N'gome-Sendeyo, K., additional, Fan, Q.-F., additional, Toblli, J., additional, Cao, G., additional, Giani, J. F., additional, Dominici, F. P., additional, Kim, J. S., additional, Yang, J. W., additional, Kim, M. K., additional, Han, B. G., additional, and Choi, S. O., additional
- Published
- 2012
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14. AKI and stem cells
- Author
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Simone, S., primary, Cariello, M., additional, Cosola, C., additional, Sallustio, F., additional, Loverre, A., additional, Schena, F. P., additional, Grandaliano, G., additional, Gesualdo, L., additional, Pertosa, G., additional, Castellano, G., additional, Curci, C., additional, Stasi, A., additional, Simone, S., additional, Montinaro, V., additional, Ditonno, P., additional, Battaglia, M., additional, Staffieri, F., additional, Crovace, A., additional, Oortwjin, B., additional, Van Amersfoort, E., additional, Weissgarten, J., additional, Efrati, S., additional, Berman, S., additional, Abu Hamad, R., additional, Christo, J. S., additional, Aparecida Reis, L., additional, Borges, F., additional, Simoes, M. d. J., additional, Schor, N., additional, Cantaluppi, V., additional, Bruno, S., additional, Figliolini, F., additional, Medica, D., additional, Tetta, C., additional, and Camussi, G., additional
- Published
- 2012
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15. Transplantation - basic
- Author
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Adamczak, M., primary, Koleganova, N., additional, Nyengaard, J. R., additional, Ritz, E., additional, Wiecek, A., additional, Slabiak Blaz, N., additional, Yi Chun, D. X., additional, Alexandre, H., additional, Sandrine, G.-S., additional, Olivier, T., additional, Isabelle, E., additional, Christophe, L., additional, Guy, T., additional, Pierre Francois, W., additional, Jean-Philippe, R., additional, Yvon, L., additional, Eric, R., additional, Muller-Krebs, S., additional, Weber, L., additional, Tsobaneli, J., additional, Reiser, J., additional, Zeier, M., additional, Schwenger, V., additional, Tinel, C., additional, Samson, M., additional, Bonnotte, B., additional, Mousson, C., additional, Machcinska, M., additional, Bocian, K., additional, Wyzgal, M., additional, Korczak-Kowalska, G., additional, Ju, M. K., additional, Huh, K. H., additional, Park, K. T., additional, Kim, S. J., additional, Cho, B. H., additional, Kim, C. D., additional, So, B. J., additional, Leee, S., additional, Kang, C. M., additional, Joo, D. J., additional, Kim, Y. S., additional, Zarzycki, M., additional, Sobich, A., additional, Matsuyama, M., additional, Hase, T., additional, Yoshimura, R., additional, Koshino, K., additional, Sakai, K., additional, Suzuki, T., additional, Nobori, S., additional, Ushigome, H., additional, Brikci-Nigassa, L., additional, Chargui, J., additional, Touraine, J.-L., additional, Yoshimura, N., additional, Cantaluppi, V., additional, Medica, D., additional, Figliolini, F., additional, Migliori, M., additional, Mannari, C., additional, Dellepiane, S., additional, Quercia, A. D., additional, Randone, O., additional, Tamagnone, M., additional, Messina, M., additional, Manzione, A. M., additional, Ranghino, A., additional, Biancone, L., additional, Segoloni, G. P., additional, Camussi, G., additional, Turk, T. R., additional, Zou, X., additional, Rauen, U., additional, De Groot, H., additional, Amann, K., additional, Kribben, A., additional, Eckardt, K.-U., additional, Bernhardt, W. M., additional, Witzke, O., additional, Lidia, G., additional, Wouter, C., additional, Eric, A., additional, Yann, L. M., additional, Christian, N., additional, Marie, E., additional, Pierre, M., additional, Zineb, A., additional, Miriana, D., additional, Annick, M., additional, Marc, A., additional, Daniel, A., additional, Wornle, M., additional, Ribeiro, A., additional, Motamedi, N., additional, Grone, H. J., additional, Cohen, C. D., additional, Schlondorff, D., additional, Schmid, H., additional, Teplan, V., additional, Banas, M., additional, Banas, B., additional, Steege, A., additional, Bergler, T., additional, Kruger, B., additional, Schnulle, P., additional, Yard, B., additional, Kramer, B. K., additional, Hoger, S., additional, Xavier, M. P., additional, Sampaio-Norton, S., additional, Gaiao, S., additional, Alves, H., additional, Oliveira, G., additional, Zaza, G., additional, Rascio, F., additional, Pontrelli, P., additional, Granata, S., additional, Rugiu, C., additional, Grandaliano, G., additional, Lupo, A., additional, Wohlfahrtova, M., additional, Brabcova, I., additional, Balaz, P., additional, Janousek, L., additional, Lodererova, A., additional, Honsova, E., additional, Wohlfahrt, P., additional, Viklicky, O., additional, Grabner, A., additional, Kentrup, D., additional, Edemir, B., additional, Sirin, Y., additional, Pavenstadt, H., additional, Schober, O., additional, Schlatter, E., additional, Schafers, M., additional, Schnockel, U., additional, Reuter, S., additional, Accetturo, M., additional, Gigante, M., additional, Tataranni, T., additional, Zito, A., additional, Schena, A., additional, Schena, F. P., additional, Stallone, G., additional, Gesualdo, L., additional, Maillard, N., additional, Masson, I., additional, Lena, A., additional, Manolie, M., additional, Christophe, M., additional, Lassen, C. K., additional, Keller, A. K., additional, Moldrup, U., additional, Bibby, B. M., additional, Jespersen, B., additional, Cvetkovic, T., additional, Velickovic Radovanovic, R., additional, Pavlovic, R., additional, Djordjevic, V., additional, Vlahovic, P., additional, Stefanovic, N., additional, Sladojevic, N., additional, Ignjatovic, A., additional, Rong, S., additional, Menne, J., additional, Haller, H., additional, Suszdak, P., additional, Tomczuk, P., additional, Gueler, F., additional, Nelli, S., additional, Sara, D., additional, Salma, E. K., additional, Naoufal, M., additional, Tarik, M., additional, Mohamed, Z., additional, Guislaine, M., additional, Mohamed Gharbi, B., additional, Benyounes, R., additional, Lu, X., additional, Shushakova, N., additional, Kirsch, T., additional, Bockmeyer, C. L., additional, Ramackers, W., additional, Wittig, J., additional, Agustian, P. A., additional, Klose, J., additional, Dammrich, M. E., additional, Kreipe, H., additional, Brocker, V., additional, Winkler, M., additional, and Becker, J. U., additional
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- 2012
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16. Transplantation basic
- Author
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Cantaluppi, V., primary, De Lena, M., additional, Figliolini, F., additional, Beltramo, S., additional, Medica, D., additional, Tognarelli, G., additional, Biancone, L., additional, Tetta, C., additional, Segoloni, G. P., additional, Camussi, G., additional, Pontrelli, P., additional, Cariello, M., additional, Verrienti, R., additional, Tataranni, T., additional, Gigante, M., additional, Loverre, A., additional, Stallone, G., additional, Schena, F. P., additional, Ranieri, E., additional, Gesualdo, L., additional, Grandaliano, G., additional, Coupel, S., additional, Charreau, B., additional, Canet, E., additional, Gerard, N., additional, Segalen, I., additional, Alexandre, N., additional, Grall, A., additional, Jacques-Olivier, P., additional, Hillion, S., additional, Le Meur, Y., additional, Alexandre, H., additional, Dany, A., additional, Michel, D., additional, Denis, G., additional, Christophe, L., additional, Nacera, O., additional, Isabelle, B., additional, Eric, R., additional, Yi Chun, D. X., additional, Buob, D., additional, Grimbert, P., additional, Glowacki, F., additional, Labalette, M., additional, Dufosse, F., additional, Nochy, D., additional, Copin, M.-C., additional, Boleslawski, E., additional, Noel, C., additional, and Hazzan, M., additional
- Published
- 2012
- Full Text
- View/download PDF
17. AKI - Experimental
- Author
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Kaynar, K., primary, Kaynar, K., additional, Ersoz, S., additional, Aliyazioglu, R., additional, Uzun, A., additional, Ulusoy, S., additional, Al, S., additional, Ozkan, G., additional, Cansiz, M., additional, Bertocchio, J.-P., additional, Lancon, J., additional, El Moghrabi, S., additional, Galmiche, G., additional, Duong Van Huyen, J.-P., additional, Rieu, P., additional, Jaisser, F., additional, Albertoni, G., additional, Andrade, S., additional, Barreto, J. A., additional, Borges, F., additional, Schor, N., additional, Ho, W.-Y., additional, Chen, S.-H., additional, Tseng, C.-J., additional, Bienholz, A., additional, Feldkamp, T., additional, Weinberg, J. M., additional, Suller Garcia, J., additional, Naves, M., additional, Aparecida Reis, L., additional, Simoes, M. d. J., additional, S Almeida, W., additional, Moreau Longo, V., additional, Segreto, H. R. C., additional, Ghoneim, A., additional, Elkholy, A., additional, Medhat Abbas, T., additional, El Hadeedy, M., additional, Elhusseini, F., additional, Elessawey, B., additional, Eltanaihy, E., additional, Lotfy, A., additional, Eldesoky, S., additional, Sheashaa, H., additional, Sobh, M., additional, Minning, D. M., additional, Warnock, D., additional, Mohamed, A. S., additional, Wirthlin, J. B., additional, Chintalacharuvu, S. R., additional, Boone, L., additional, Brenner, R. M., additional, Santina Christo, J., additional, Dos Santos Passos, C., additional, Rene de Alencar, D., additional, De Braganca, A. C., additional, Canale, D., additional, Goncalves, J. G., additional, Brandao, T. P. B., additional, Shimizu, M. H. M., additional, Volpini, R. A., additional, Seguro, A. C., additional, Andrade, L., additional, Lee, J.-W., additional, Kim, H. K., additional, Cho, W. Y., additional, Jo, S.-K., additional, Cho, E., additional, Hocherl, K., additional, Schmidt, C., additional, Mulay, S. R., additional, Kulkarni, O. P., additional, Rupanagudi, K. V., additional, Migliorini, A., additional, Liapis, H., additional, Anders, H.-J., additional, Pevzner, I., additional, Chupyrkina, A., additional, Plotnikov, E., additional, Zorov, D., additional, Lopez-Novoa, J.-M., additional, Eleno, N., additional, Perez-Barriocanal, F., additional, Arevalo, M., additional, Docherty, N., additional, Castellano, G., additional, Divella, C., additional, Loverre, A., additional, Stasi, A., additional, Curci, C., additional, Rossini, M., additional, Ditonno, P., additional, Battaglia, M., additional, Daha, M. R., additional, Van Kooten, C., additional, Gesualdo, L., additional, Schena, F. P., additional, Grandaliano, G., additional, Tsuda, H., additional, Kawada, N., additional, Iwatani, H., additional, Moriyama, T., additional, Takahara, S., additional, Rakugi, H., additional, Isaka, Y., additional, Schley, G., additional, Kalucka, J., additional, Klanke, B., additional, Jantsch, J., additional, Olbrich, S., additional, Baumgartl, J., additional, Amann, K., additional, Eckardt, K.-U., additional, Weidemann, A., additional, Dolgolikova, A., additional, Pilotovich, V., additional, Ivanchik, G., additional, Shved, I., additional, Banki, N. F., additional, Antal, Z., additional, Hosszu, A., additional, Koszegi, S., additional, Vannay, A., additional, Wagner, L., additional, Prokai, A., additional, Muller, V., additional, Szabo, A. J., additional, Fekete, A., additional, Farrag, S., additional, Abulasrar, S., additional, Salama, , M., additional, Amin, M., additional, Ali, A., additional, Rubera, I., additional, Duranton, C., additional, Cougnon, M., additional, Melis, N., additional, Tauc, M., additional, Jankauskas, S., additional, Morosanova, M., additional, Pulkina, N., additional, Zorova, L., additional, Shin, Y. T., additional, Kim, S. S., additional, Chang, Y. K., additional, Choi, D. E., additional, Na, K.-R., additional, Lee, K. W., additional, Choi, J.-Y., additional, Jin, D.-C., additional, Cha, J.-H., additional, Schneider, R., additional, Betz, B., additional, Meusel, M., additional, Held, C., additional, Wanner, C., additional, Gekle, M., additional, Sauvant, C., additional, Pisani, A., additional, Rossano, R., additional, Mancini, A., additional, Arfian, N., additional, Yagi, K., additional, Nakayama, K., additional, Ali, H., additional, Mayasari, D. S., additional, Purnomo, E., additional, Emoto, N., additional, Efrati, S., additional, Berman, S., additional, Abu Hamad, R., additional, Weissgarten, J., additional, Scherbaum, C. R., additional, Allam, R., additional, Lichtnekert, J., additional, Darisipudi, M. N., additional, Hagele, H., additional, Hohenstein, B., additional, Hugo, C., additional, Schaefer, L., additional, Corsi, C., additional, Ferramosca, E., additional, Grandi, E., additional, Pisoni, L., additional, Rivolta, I., additional, Dalpozzo, B., additional, Hoxha, E., additional, Severi, S., additional, Santoro, A., additional, Laurent, M., additional, Cedric, R., additional, Dominique, C., additional, Sophie, V., additional, Nochy, D., additional, Loic, G., additional, Patrice, C., additional, Chantal, J., additional, Marie-Christine, V., additional, Alexandre, H., additional, Eric, R., additional, Cantaluppi, V., additional, Medica, D., additional, Quercia, A. D., additional, Figliolini, F., additional, Dellepiane, S., additional, Randone, O., additional, Segoloni, G. P., additional, Camussi, G., additional, Ahn, B.-H., additional, Kim, S. H., additional, Yasue Saito Miyagi, M., additional, Camara, N., additional, Cerqueira Leite Seelaender, M., additional, Maceratesi Enjiu, L., additional, Estler Rocha Guilherme, P., additional, Pisciottano, M., additional, Hiyane, M., additional, Yuri Hayashida, C., additional, De Andrade Oliveira, V., additional, Olsen Saraiva Camara, N., additional, Tami Amano, M., additional, Sancho-Martinez, S. M., additional, Sanchez-Juanes, F., additional, Vicente, L., additional, Gonzalez-Buitrago, J. M., additional, Morales, A. I., additional, Lopez-Novoa, J. M., additional, Lopez-Hernandez, F. J., additional, Chen, J.-S., additional, Chang, L.-C., additional, Chen, C.-C., additional, Park, M. Y., additional, Choi, S. J., additional, Kim, J. G., additional, Hwang, S. D., additional, Vicente-Vicente, L., additional, Ferreira, L., additional, Prieto, M., additional, Garcia-Sanchez, O., additional, Sevilla, M. A., additional, Lopez-Novoa, F. J., additional, Christoph, K., additional, Kuper, C., additional, Maria-Luisa, F., additional, Franz-Xaver, B., additional, Neuhofer, W., additional, Vervaet, B., additional, Le Clef, N., additional, Verhulst, A., additional, D'haese, P., additional, Tanaka, T., additional, Yamaguchi, J., additional, Eto, N., additional, Kojima, I., additional, Fujita, T., additional, Nangaku, M., additional, Wystrychowski, A., additional, Wystrychowski, G., additional, Obuchowicz, E., additional, Grzeszczak, W., additional, Wiecek, A., additional, Esposito, C., additional, Torreggiani, M., additional, Castoldi, F., additional, Migotto, C., additional, Serpieri, N., additional, Grosjean, F., additional, Manini, A., additional, Pertile, E., additional, and Dal Canton, A., additional
- Published
- 2012
- Full Text
- View/download PDF
18. Acute Tubular Injury is Associated With Severe Traumatic Brain Injury: in Vitro Study on Human Tubular Epithelial Cells
- Author
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Vito Marco Ranieri, Luciana Mascia, Vincenzo Cantaluppi, Barbara Assenzio, Vito Fanelli, Davide Medica, Ilaria Deambrosis, Fulvia Giaretta, Federica Civiletti, Anna Teresa Mazzeo, Civiletti F., Assenzio B., Mazzeo A.T., Medica D., Giaretta F., Deambrosis I., Fanelli V., Ranieri V.M., Cantaluppi V., and Mascia L.
- Subjects
0301 basic medicine ,Male ,Pathology ,lcsh:Medicine ,Apoptosis ,Lipocalin ,Kidney Tubules, Proximal ,chemistry.chemical_compound ,0302 clinical medicine ,Brain Injuries, Traumatic ,Medicine ,Prospective Studies ,lcsh:Science ,Cells, Cultured ,Kidney ,Multidisciplinary ,Acute kidney injury ,Acute Kidney Injury ,Middle Aged ,Lipocalins ,Low Density Lipoprotein Receptor-Related Protein-2 ,medicine.anatomical_structure ,Cytokines ,Female ,Human ,Adult ,medicine.medical_specialty ,Traumatic brain injury ,Urinary system ,Acute Kidney Injury, Lipocalins, Neutrophil gelatinase-associated ,Article ,03 medical and health sciences ,Young Adult ,Lipocalin-2 ,Humans ,Viability assay ,Cytokine ,Creatinine ,Epithelial Cell ,business.industry ,lcsh:R ,Neutrophil gelatinase-associated ,Brain injuries, epithelial cells ,Apoptosi ,Epithelial Cells ,Biomarker ,medicine.disease ,Acute Kidney Injury | Lipocalins | Neutrophil gelatinase-associated ,Prospective Studie ,030104 developmental biology ,chemistry ,lcsh:Q ,business ,Biomarkers ,030217 neurology & neurosurgery - Abstract
Acute kidney injury following traumatic brain injury is associated with poor outcome. We investigated in vitro the effects of plasma of brain injured patients with acute tubular kidney injury on kidney tubular epithelial cell function. we performed a prospective observational clinical study in ICU in a trauma centre of the University hospital in Italy including twenty-three ICU patients with traumatic brain injury consecutively enrolled. Demographic data were recorded on admission: age 39 ± 19, Glasgow Coma Score 5 (3–8). Neutrophil Gelatinase-Associated Lipocalin and inflammatory mediators were measured in plasma on admission and after 24, 48 and 72 hours; urine were collected for immunoelectrophoresis having healthy volunteers as controls. Human renal proximal tubular epithelial cells were stimulated with patients or controls plasma. Adhesion of freshly isolated human neutrophils and trans-epithelial electrical resistance were assessed; cell viability (XTT assay), apoptosis (TUNEL staining), Neutrophil Gelatinase-Associated Lipocalin and Megalin expression (quantitative real-time PCR) were measured. All patients with normal serum creatinine showed increased plasmatic Neutrophil Gelatinase-Associated Lipocalin and increased urinary Retinol Binding Protein and α1-microglobulin. Neutrophil Gelatinase-Associated Lipocalin was significantly correlated with both inflammatory mediators and markers of tubular damage. Patient’ plasma incubated with tubular cells significantly increased adhesion of neutrophils, reduced trans-epithelial electrical resistance, exerted a cytotoxic effect and triggered apoptosis and down-regulated the endocytic receptor Megalin compared to control. Plasma of brain injured patients with increased markers of subclinical acute kidney induced a pro-inflammatory phenotype, cellular dysfunction and apoptotic death in tubular epithelial cells.
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- 2019
19. Individualized homeopathic medicines in preventing the progression from pre-diabetes to diabetes: A double-blind, randomized, placebo-controlled, parallel-arm trial.
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Banerjee A, Ganguly S, Saha S, Bhattacharyya P, Naskar S, Mukherjee D, Ghosh S, Maji P, Saha S, Shaikh AR, Ghosh P, Chatterjee C, Koley M, and Mukherjee SK
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- Humans, Double-Blind Method, Male, Female, Middle Aged, Adult, India, Homeopathy methods, Yoga, Glucose Tolerance Test, Diabetes Mellitus, Type 2 drug therapy, Treatment Outcome, Prediabetic State drug therapy, Blood Glucose drug effects, Blood Glucose metabolism, Glycated Hemoglobin metabolism, Disease Progression, Materia Medica therapeutic use
- Abstract
Context: Pre-diabetes is a significant public health problem worldwide. India has a very high rate of progression from pre-diabetes to diabetes, 75-78 per thousand persons per year., Objective: To study the efficacy of individualized homeopathic medicinal products (HMPs) against placebos in preventing the progression from pre-diabetes to diabetes., Design: Six-month, double-blind, randomized (1:1), two parallel arms, placebo-controlled trial., Setting: Outpatient departments of D. N. De Homoeopathic Medical College and Hospital, Kolkata, West Bengal, India., Patients: Sixty participants with pre-diabetes., Interventions: Verum: HMPs plus yoga therapy (YT; n = 30); control: identical-looking placebos plus YT (n = 30)., Main Outcome Measures: The primary efficacy endpoint was the proportion of participants progressing from pre-diabetes to diabetes, measured after three and six months. Secondary outcomes comprised of fasting blood glucose (FBS), oral glucose tolerance test (OGTT), glycated hemoglobin percentage (HbA1c%), lipid profile, liver enzymes (alanine transaminase, aspartate transaminase), urea and creatinine, and Measure Yourself Medical Outcome Profile version 2 (MYMOP-2); all measured after 3 and 6 months., Results: The proportion of participants converted from pre-diabetics to diabetics (n/N; n = diabetics, N = prediabetics) was significantly less in the verum group than control: HbA1C% (month 3: verum - 2/30 versus control - 11/30, p = 0.003; month 6: 3/30 vs. 2/30, p = 0.008), OGTT (month 3: 0/30 vs. 8/30, p = 0.015; month 6: 0/30 vs. 1/30, p = 0.008), but not according to FBS (month 3: 1/30 vs. 1/30, p = 0.779; month 6: 1/30 vs. 3/30, p = 0.469). Several secondary outcomes also revealed significant improvements in the verum group than in placebo: HbA1C% (p < 0.001), OGTT (p = 0.001), serum ALT (p = 0.031), creatinine (p = 0.012), and MYMOP-2 profile scores (p < 0.001). Sulphur, Bryonia alba, and Thuja occidentalis were the most frequently indicated medicines. Thus, HMPs outperformed placebos by successfully preventing the progression of pre-diabetes to diabetes., Trial Registration: Clinical Trials Registry - India CTRI/2022/04/042,026; UTN: U1111-1277-0021., (Copyright © 2024 Elsevier Inc. All rights reserved.)
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- 2024
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20. High-volume hemofiltration does not protect human kidney endothelial and tubular epithelial cells from septic plasma-induced injury.
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Medica D, Quercia AD, Marengo M, Fanelli V, Castellano G, Fabbrini P, Migliori M, Merlotti G, Camussi G, Joannes-Boyau O, Honorè PM, and Cantaluppi V
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- Humans, Male, Acute Kidney Injury therapy, Acute Kidney Injury etiology, Female, Middle Aged, Apoptosis, Aged, Kidney Tubules metabolism, Cytokines metabolism, Cytokines blood, Cell Adhesion, Sepsis therapy, Endothelial Cells metabolism, Hemofiltration methods, Epithelial Cells metabolism
- Abstract
High volume hemofiltration (HVHF) could remove from plasma inflammatory mediators involved in sepsis-associated acute kidney injury (SA-AKI). The IVOIRE trial did not show improvements of outcome and organ dysfunction using HVHF. The aim of this study was to evaluate in vitro the biological effects of plasma of patients treated by HVHF or standard volume hemofiltration (SVHF). We evaluated leukocyte adhesion, apoptosis and functional alterations of endothelial cells (EC) and tubular epithelial cells (TEC). In vitro data were correlated with plasma levels of TNF-α, Fas-Ligand (FasL), CD40-Ligand (CD40L), von Willebrand Factor (vWF) and endothelial-derived microparticles. An experimental model of in vitro hemofiltration using LPS-activated blood was established to assess cytokine mass adsorption during HVHF or SVHF. Plasma concentrations of TNF-ɑ, FasL, CD40L and von Willebrand Factor (vWF) were elevated at the start (d1h0) of both HVHF and SVHF, significantly decreased after 6 h (d1h6), remained stable after 12 h (d1h12) and then newly increased at 48 h (d3h0). Plasma levels of all these molecules were similar between HVHF- and SVHF-treated patients at all time points considered. In addition, the levels of endothelial microparticles remained always elevated, suggesting the presence of a persistent microvascular injury. Plasma from septic patients induced leukocyte adhesion on EC and TEC through up-regulation of adhesion receptors. Moreover, on EC, septic plasma induced a cytotoxic and anti-angiogenic effect. On TEC, septic plasma exerted a direct pro-apoptotic effect via Fas up-regulation and caspase activation, loss of polarity, altered expression of megalin and tight junction molecules with an impaired ability to internalize albumin. The inhibition of plasma-induced cell injury was concomitant to the decrease of TNF-α, Fas-Ligand and CD40-Ligand levels. The protective effect of both HVHF and SVHF was time-limited, since a further increase of circulating mediators and plasma-induced cell injury was observed after 48 h (d3h0). No significant difference of EC/TEC damage were observed using HVHF- or SVHF-treated plasma. The in vitro hemofiltration model confirmed the absence of a significant modulation of cytokine adsorption between HVHF and SVHF. In comparison to SVHF, HVHF did not increase inflammatory cytokine clearance and did not reverse the detrimental effects of septic plasma-induced EC and TEC injury. Further studies using adsorptive membranes are needed to evaluate the potential role of high dose convective therapies in the limitation of the harmful activity of plasma soluble factors involved in SA-AKI.Trial registration IVOIRE randomized clinical trial; ClinicalTrials.gov (NCT00241228) (18/10/2005)., (© 2024. The Author(s).)
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- 2024
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21. Finding the Needle in the Haystack: Serological and Urinary Biomarkers in Behçet's Disease: A Systematic Review.
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Arbrile M, Radin M, Medica D, Miraglia P, Rilat L, Cecchi I, Foddai SG, Barinotti A, Menegatti E, Roccatello D, and Sciascia S
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- Humans, Retrospective Studies, Prospective Studies, Case-Control Studies, Biomarkers, Behcet Syndrome diagnosis
- Abstract
Urinary and serological markers play an essential role in the diagnostic process of autoimmune diseases. However, to date, specific and reliable biomarkers for diagnosing Behçet's disease (BD) are still lacking, negatively affecting the management of these patients. To analyze the currently available literature on serological and urinary BD biomarkers investigated in the last 25 years, we performed a systematic literature review using the Population, Intervention, Comparison, and Outcomes (PICO) strategy. One hundred eleven studies met the eligibility criteria (6301 BD patients, 5163 controls). Most of them were retrospective, while five (5%) were prospective. One hundred ten studies (99%) investigated serological biomarkers and only two (2%) focused on urinary biomarkers. One hundred three studies (93%) explored the diagnostic potential of the biomolecules, whereas sixty-two (56%) tested their effect on disease activity monitoring. Most articles reported an increase in inflammatory markers and pro-oxidant molecules, with a decrease in antioxidants. Promising results have been shown by the omics sciences, offering a more holistic approach. Despite the vast number of investigated markers, existing evidence indicates a persistent gap in BD diagnostic/prognostic indices. While new steps have been taken in the direction of pathogenesis and disease monitoring, international efforts for the search of a diagnostic marker for BD are still needed.
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- 2023
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22. Extracellular Vesicles Derived from Endothelial Progenitor Cells Protect Human Glomerular Endothelial Cells and Podocytes from Complement- and Cytokine-Mediated Injury.
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Medica D, Franzin R, Stasi A, Castellano G, Migliori M, Panichi V, Figliolini F, Gesualdo L, Camussi G, and Cantaluppi V
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- Apoptosis drug effects, Apoptosis genetics, Cell Movement drug effects, Cell Proliferation drug effects, Coculture Techniques, Endothelial Progenitor Cells cytology, Endothelial Progenitor Cells metabolism, Extracellular Vesicles chemistry, Gene Expression Regulation, Hepatocyte Growth Factor genetics, Hepatocyte Growth Factor metabolism, Humans, Intercellular Adhesion Molecule-1 genetics, Intercellular Adhesion Molecule-1 metabolism, L-Selectin genetics, L-Selectin metabolism, MicroRNAs genetics, MicroRNAs metabolism, Neovascularization, Physiologic drug effects, Neovascularization, Physiologic genetics, Paracrine Communication drug effects, Podocytes cytology, Podocytes metabolism, Primary Cell Culture, Reactive Oxygen Species antagonists & inhibitors, Reactive Oxygen Species metabolism, Vascular Cell Adhesion Molecule-1 genetics, Vascular Cell Adhesion Molecule-1 metabolism, Vascular Endothelial Growth Factor A genetics, Vascular Endothelial Growth Factor A metabolism, Complement C5a pharmacology, Endothelial Progenitor Cells drug effects, Extracellular Vesicles metabolism, Interleukin-6 pharmacology, Podocytes drug effects, Tumor Necrosis Factor-alpha pharmacology
- Abstract
Glomerulonephritis are renal inflammatory processes characterized by increased permeability of the Glomerular Filtration Barrier (GFB) with consequent hematuria and proteinuria. Glomerular endothelial cells (GEC) and podocytes are part of the GFB and contribute to the maintenance of its structural and functional integrity through the release of paracrine mediators. Activation of the complement cascade and pro-inflammatory cytokines (CK) such as Tumor Necrosis Factor α (TNF-α) and Interleukin-6 (IL-6) can alter GFB function, causing acute glomerular injury and progression toward chronic kidney disease. Endothelial Progenitor Cells (EPC) are bone-marrow-derived hematopoietic stem cells circulating in peripheral blood and able to induce angiogenesis and to repair injured endothelium by releasing paracrine mediators including Extracellular Vesicles (EVs), microparticles involved in intercellular communication by transferring proteins, lipids, and genetic material (mRNA, microRNA, lncRNA) to target cells. We have previously demonstrated that EPC-derived EVs activate an angiogenic program in quiescent endothelial cells and renoprotection in different experimental models. The aim of the present study was to evaluate in vitro the protective effect of EPC-derived EVs on GECs and podocytes cultured in detrimental conditions with CKs (TNF-α/IL-6) and the complement protein C5a. EVs were internalized in both GECs and podocytes mainly through a L-selectin-based mechanism. In GECs, EVs enhanced the formation of capillary-like structures and cell migration by modulating gene expression and inducing the release of growth factors such as VEGF-A and HGF. In the presence of CKs, and C5a, EPC-derived EVs protected GECs from apoptosis by decreasing oxidative stress and prevented leukocyte adhesion by inhibiting the expression of adhesion molecules (ICAM-1, VCAM-1, E-selectin). On podocytes, EVs inhibited apoptosis and prevented nephrin shedding induced by CKs and C5a. In a co-culture model of GECs/podocytes that mimicked GFB, EPC-derived EVs protected cell function and permeselectivity from inflammatory-mediated damage. Moreover, RNase pre-treatment of EVs abrogated their protective effects, suggesting the crucial role of RNA transfer from EVs to damaged glomerular cells. In conclusion, EPC-derived EVs preserved GFB integrity from complement- and cytokine-induced damage, suggesting their potential role as therapeutic agents for drug-resistant glomerulonephritis.
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- 2021
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23. PMMA-Based Continuous Hemofiltration Modulated Complement Activation and Renal Dysfunction in LPS-Induced Acute Kidney Injury.
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Stasi A, Franzin R, Divella C, Sallustio F, Curci C, Picerno A, Pontrelli P, Staffieri F, Lacitignola L, Crovace A, Cantaluppi V, Medica D, Ronco C, de Cal M, Lorenzin A, Zanella M, Pertosa GB, Stallone G, Gesualdo L, and Castellano G
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- Acute Kidney Injury diagnosis, Acute Kidney Injury mortality, Animals, Biomarkers, C-Reactive Protein genetics, C-Reactive Protein metabolism, Disease Models, Animal, Fibrosis, Gene Expression, Humans, Immunohistochemistry, Inflammation Mediators, Kidney Function Tests, Renal Dialysis, Sepsis complications, Serum Amyloid P-Component genetics, Serum Amyloid P-Component metabolism, Swine, Treatment Outcome, Acute Kidney Injury etiology, Acute Kidney Injury therapy, Complement Activation drug effects, Hemofiltration adverse effects, Hemofiltration methods, Lipopolysaccharides adverse effects, Polymethyl Methacrylate administration & dosage
- Abstract
Sepsis-induced acute kidney injury (AKI) is a frequent complication in critically ill patients, refractory to conventional treatments. Aberrant activation of innate immune system may affect organ damage with poor prognosis for septic patients. Here, we investigated the efficacy of polymethyl methacrylate membrane (PMMA)-based continuous hemofiltration (CVVH) in modulating systemic and tissue immune activation in a swine model of LPS-induced AKI. After 3 h from LPS infusion, animals underwent to PMMA-CVVH or polysulfone (PS)-CVVH. Renal deposition of terminal complement mediator C5b-9 and of Pentraxin-3 (PTX3) deposits were evaluated on biopsies whereas systemic Complement activation was assessed by ELISA assay. Gene expression profile was performed from isolated peripheral blood mononuclear cells (PBMC) by microarrays and the results validated by Real-time PCR. Endotoxemic pigs presented oliguric AKI with increased tubulo-interstitial infiltrate, extensive collagen deposition, and glomerular thrombi; local PTX-3 and C5b-9 renal deposits and increased serum activation of classical and alternative Complement pathways were found in endotoxemic animals. PMMA-CVVH treatment significantly reduced tissue and systemic Complement activation limiting renal damage and fibrosis. By microarray analysis, we identified 711 and 913 differentially expressed genes with a fold change >2 and a false discovery rate <0.05 in endotoxemic pigs and PMMA-CVVH treated-animals, respectively. The most modulated genes were Granzyme B, Complement Factor B, Complement Component 4 Binding Protein Alpha, IL-12, and SERPINB-1 that were closely related to sepsis-induced immunological process. Our data suggest that PMMA-based CVVH can efficiently modulate immunological dysfunction in LPS-induced AKI., Competing Interests: GC reports research grant donation from TORAY (Toray Industries, Inc), during the conduct of the study. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2021 Stasi, Franzin, Divella, Sallustio, Curci, Picerno, Pontrelli, Staffieri, Lacitignola, Crovace, Cantaluppi, Medica, Ronco, de Cal, Lorenzin, Zanella, Pertosa, Stallone, Gesualdo and Castellano.)
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- 2021
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24. Targeted and untargeted quantification of quorum sensing signalling molecules in bacterial cultures and biological samples via HPLC-TQ MS techniques.
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Dal Bello F, Zorzi M, Aigotti R, Medica D, Fanelli V, Cantaluppi V, Amante E, Orlandi VT, and Medana C
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- Acyl-Butyrolactones chemistry, Humans, Limit of Detection, Molecular Structure, Multiple Organ Failure blood, Multiple Organ Failure etiology, Quinolones chemistry, Reproducibility of Results, Sepsis blood, Sepsis complications, Sepsis microbiology, Virulence Factors blood, Acyl-Butyrolactones analysis, Chromatography, High Pressure Liquid methods, Mass Spectrometry methods, Pseudomonas aeruginosa metabolism, Quinolones analysis, Quorum Sensing, Signal Transduction
- Abstract
Quorum sensing (QS) is the ability of some bacteria to detect and to respond to population density through signalling molecules. QS molecules are involved in motility and cell aggregation mechanisms in diseases such as sepsis. Few biomarkers are currently available to diagnose sepsis, especially in high-risk conditions. The aim of this study was the development of new analytical methods based on liquid chromatography-mass spectrometry for the detection and quantification of QS signalling molecules, including N-acyl homoserine lactones (AHL) and hydroxyquinolones (HQ), in biofluids. Biological samples used in the study were Pseudomonas aeruginosa bacterial cultures and plasma from patients with sepsis. We developed two MS analytical methods, based on neutral loss (NL) and product ion (PI) experiments, to identify and characterize unknown AHL and HQ molecules. We then established a multiple-reaction-monitoring (MRM) method to quantify specific QS compounds. We validated the HPLC-MS-based approaches (MRM-NL-PI), and data were in accord with the validation guidelines. With the NL and PI MS-based methods, we identified and characterized 3 and 13 unknown AHL and HQ compounds, respectively, in biological samples. One of the newly found AHL molecules was C12-AHL, first quantified in Pseudomonas aeruginosa bacterial cultures. The MRM quantitation of analytes in plasma from patients with sepsis confirmed the analytical ability of MRM for the quantification of virulence factors during sepsis. Graphical abstract.
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- 2021
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25. Citrate anion improves chronic dialysis efficacy, reduces systemic inflammation and prevents Chemerin-mediated microvascular injury.
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Dellepiane S, Medica D, Guarena C, Musso T, Quercia AD, Leonardi G, Marengo M, Migliori M, Panichi V, Biancone L, Pizzarelli F, Camussi G, and Cantaluppi V
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- C-Reactive Protein analysis, Chemokines blood, Endothelium, Vascular injuries, Endothelium, Vascular metabolism, Female, Fibrinogen analysis, Hemodialysis Solutions, Humans, Inflammation etiology, Interleukin-6 blood, Male, Microvessels metabolism, Middle Aged, Muscle, Smooth, Vascular injuries, Muscle, Smooth, Vascular metabolism, Renal Dialysis methods, Treatment Outcome, Chemokines metabolism, Citric Acid therapeutic use, Inflammation prevention & control, Microvessels injuries, Renal Dialysis adverse effects
- Abstract
Systemic inflammation and uremic toxins (UT) determine the increased cardiovascular mortality observed in chronic hemodialysis (HD) patients. Among UT, the adipokine Chemerin induces vascular dysfunction by targeting both endothelial and vascular smooth muscular cells (EC and VSMC). As Citrate anion modulates oxidative metabolism, systemic inflammation and vascular function, we evaluated whether citrate-buffered dialysis improves HD efficiency, inflammatory parameters and chemerin-mediated microvascular injury. 45 patients were treated in sequence with acetate, citrate and, again, acetate-buffered dialysis solution (3 months per interval). At study admission and after each treatment switch, we evaluated dialysis efficacy and circulating levels of chemerin and different inflammatory biomarkers. In vitro, we stimulated EC and VSMC with patients' plasma and we investigated the role of chemerin as UT. Citrate dialysis increased HD efficacy and reduced plasma levels of CRP, fibrinogen, IL6 and chemerin. In vitro, patients' plasma induced EC and VSMC dysfunction. These effects were reduced by citrate-buffered solutions and paralleled by the decrease of chemerin levels. Consistently, chemerin receptor knockdown reduced EC and VSMC dysfunction. In conclusion, Switching from acetate to citrate improved dialysis efficacy and inflammatory parameters; in vitro, chemerin-induced EC and VSMC injury were decreased by using citrate as dialysis buffer.
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- 2019
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26. Acute Tubular Injury is Associated With Severe Traumatic Brain Injury: in Vitro Study on Human Tubular Epithelial Cells.
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Civiletti F, Assenzio B, Mazzeo AT, Medica D, Giaretta F, Deambrosis I, Fanelli V, Ranieri VM, Cantaluppi V, and Mascia L
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- Adult, Apoptosis, Biomarkers blood, Cells, Cultured, Cytokines blood, Female, Humans, Kidney Tubules, Proximal pathology, Lipocalin-2 blood, Low Density Lipoprotein Receptor-Related Protein-2 blood, Male, Middle Aged, Prospective Studies, Young Adult, Acute Kidney Injury etiology, Brain Injuries, Traumatic complications, Epithelial Cells pathology, Kidney Tubules, Proximal cytology
- Abstract
Acute kidney injury following traumatic brain injury is associated with poor outcome. We investigated in vitro the effects of plasma of brain injured patients with acute tubular kidney injury on kidney tubular epithelial cell function. we performed a prospective observational clinical study in ICU in a trauma centre of the University hospital in Italy including twenty-three ICU patients with traumatic brain injury consecutively enrolled. Demographic data were recorded on admission: age 39 ± 19, Glasgow Coma Score 5 (3-8). Neutrophil Gelatinase-Associated Lipocalin and inflammatory mediators were measured in plasma on admission and after 24, 48 and 72 hours; urine were collected for immunoelectrophoresis having healthy volunteers as controls. Human renal proximal tubular epithelial cells were stimulated with patients or controls plasma. Adhesion of freshly isolated human neutrophils and trans-epithelial electrical resistance were assessed; cell viability (XTT assay), apoptosis (TUNEL staining), Neutrophil Gelatinase-Associated Lipocalin and Megalin expression (quantitative real-time PCR) were measured. All patients with normal serum creatinine showed increased plasmatic Neutrophil Gelatinase-Associated Lipocalin and increased urinary Retinol Binding Protein and α1-microglobulin. Neutrophil Gelatinase-Associated Lipocalin was significantly correlated with both inflammatory mediators and markers of tubular damage. Patient' plasma incubated with tubular cells significantly increased adhesion of neutrophils, reduced trans-epithelial electrical resistance, exerted a cytotoxic effect and triggered apoptosis and down-regulated the endocytic receptor Megalin compared to control. Plasma of brain injured patients with increased markers of subclinical acute kidney induced a pro-inflammatory phenotype, cellular dysfunction and apoptotic death in tubular epithelial cells.
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- 2019
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27. Perfluorocarbon solutions limit tubular epithelial cell injury and promote CD133+ kidney progenitor differentiation: potential use in renal assist devices for sepsis-associated acute kidney injury and multiple organ failure.
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Cantaluppi V, Medica D, Quercia AD, Dellepiane S, Figliolini F, Virzì GM, Brocca A, Quaglia M, Marengo M, Olivieri C, Senzolo M, Garzotto F, Della Corte F, Castellano G, Gesualdo L, Camussi G, and Ronco C
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- Acute Kidney Injury diagnosis, Acute Kidney Injury etiology, Aged, Aged, 80 and over, Animals, Cell Differentiation drug effects, Cell Proliferation drug effects, Cells, Cultured, Female, Humans, Kidney Tubules drug effects, Kidney Tubules metabolism, Kidney Tubules pathology, Male, Middle Aged, Multiple Organ Failure diagnosis, Multiple Organ Failure etiology, Sepsis pathology, Sepsis therapy, Stem Cells drug effects, Stem Cells metabolism, AC133 Antigen metabolism, Acute Kidney Injury therapy, Apoptosis drug effects, Fluorocarbons pharmacology, Multiple Organ Failure therapy, Sepsis complications, Stem Cells pathology
- Abstract
Background: The renal assist device (RAD) is a blood purification system containing viable renal tubular epithelial cells (TECs) that has been proposed for the treatment of acute kidney injury (AKI) and multiple organ failure. Perfluorocarbons (PFCs) are oxygen carriers used for organ preservation in transplantation. The aim of this study was to investigate the effect of PFCs on hypoxia- and sepsis-induced TEC injury and on renal CD133+ progenitor differentiation in a microenvironment similar to the RAD., Methods: TECs were seeded in a polysulphone hollow fibre under hypoxia or cultured with plasma from 10 patients with sepsis-associated AKI in the presence or absence of PFCs and were tested for cytotoxicity (XTT assay), apoptosis (terminal deoxynucleotidyl transferase dUTP nick end labeling assay, caspases, enzyme-linked immunosorbent assay, Fas/Fas Ligand pathway activation), mitochondrial activity, cell polarity [transepithelial electrical resistance (TEER)] and adenosine triphosphate production. The effect of PFCs on proliferation and differentiation of human CD133+ progenitors was also studied., Results: In the presence of PFCs, TECs seeded into the polysulphone hollow fibre showed increased viability and expression of insulin-like growth factor 1, hepatocyte growth factor and macrophage-stimulating protein. Plasma from septic patients induced TEC apoptosis, disruption of oxidative metabolism, alteration of cell polarity and albumin uptake, down-regulation of the tight junction protein ZO-1 and the endocytic receptor megalin on the TEC surface. These detrimental effects were significantly reduced by PFCs. Moreover, PFCs induced CD133+ renal progenitor cell proliferation and differentiation towards an epithelial/tubular-like phenotype., Conclusions: PFCs improved the viability and metabolic function of TECs seeded within a polysulphone hollow fibre and subjected to plasma from septic AKI patients. Additionally, PFCs promoted differentiation towards a tubular/epithelial phenotype of CD133+ renal progenitor cells.
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- 2018
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28. Neutrophil Gelatinase Associated Lipocalin Is an Early and Accurate Biomarker of Graft Function and Tissue Regeneration in Kidney Transplantation from Extended Criteria Donors.
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Cantaluppi V, Dellepiane S, Tamagnone M, Medica D, Figliolini F, Messina M, Manzione AM, Gai M, Tognarelli G, Ranghino A, Dolla C, Ferrario S, Tetta C, Segoloni GP, Camussi G, and Biancone L
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- Acute-Phase Proteins genetics, Aged, Apoptosis drug effects, Biomarkers blood, Cell Hypoxia, Cells, Cultured, Cohort Studies, Delayed Graft Function blood, Delayed Graft Function etiology, Donor Selection, Female, Gene Expression, Humans, Immunosuppressive Agents adverse effects, Kidney drug effects, Kidney physiopathology, Kidney Tubules drug effects, Kidney Tubules pathology, Kidney Tubules physiopathology, Lipocalin-2, Lipocalins genetics, Male, Middle Aged, Prospective Studies, Proto-Oncogene Proteins genetics, Regeneration, Tacrolimus adverse effects, Kidney Transplantation adverse effects, Lipocalins blood, Proto-Oncogene Proteins blood, Tissue Donors
- Abstract
Background: Delayed graft function (DGF) is an early complication of kidney transplantation (KT) associated with increased risk of early loss of graft function. DGF increases using kidneys from extended criteria donors (ECD). NGAL is a 25KDa protein proposed as biomarker of acute kidney injury. The aim of this study was to investigate the role of NGAL as an early and accurate indicator of DGF and Tacrolimus (Tac) toxicity and as a mediator of tissue regeneration in KT from ECD., Methods: We evaluated plasma levels of NGAL in 50 KT patients from ECD in the first 4 days after surgery or after Tac introduction., Results: Plasma levels of NGAL at day 1 were significantly higher in DGF group. In the non DGF group, NGAL discriminated between slow or immediate graft function and decreased more rapidly than serum creatinine. NGAL increased after Tac introduction, suggesting a role as marker of drug toxicity. In vitro, hypoxia and Tac induced NGAL release from tubular epithelial cells (TEC) favoring an autocrine loop that sustains proliferation and inhibits apoptosis (decrease of caspases and Bax/Bcl-2 ratio)., Conclusions: NGAL is an early and accurate biomarker of graft function in KT from ECD favoring TEC regeneration after ischemic and nephrotoxic injury.
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- 2015
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29. Caffeic acid, a phenol found in white wine, modulates endothelial nitric oxide production and protects from oxidative stress-associated endothelial cell injury.
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Migliori M, Cantaluppi V, Mannari C, Bertelli AA, Medica D, Quercia AD, Navarro V, Scatena A, Giovannini L, Biancone L, and Panichi V
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- Animals, Apoptosis drug effects, Cell Adhesion drug effects, Cell Hypoxia drug effects, Cell Proliferation drug effects, Gene Expression Regulation, Enzymologic drug effects, Granulocytes immunology, Human Umbilical Vein Endothelial Cells cytology, Humans, Kidney Tubules drug effects, Kidney Tubules immunology, Kidney Tubules injuries, Mice, Neovascularization, Physiologic drug effects, Nitric Oxide metabolism, Nitric Oxide Synthase Type III metabolism, Phosphorylation drug effects, Reactive Oxygen Species metabolism, Reperfusion Injury immunology, Reperfusion Injury metabolism, Reperfusion Injury pathology, Uremia metabolism, Uremia pathology, Antioxidants pharmacology, Caffeic Acids pharmacology, Human Umbilical Vein Endothelial Cells drug effects, Human Umbilical Vein Endothelial Cells metabolism, Nitric Oxide biosynthesis, Oxidative Stress drug effects, Wine analysis
- Abstract
Introduction: Several studies demonstrated that endothelium dependent vasodilatation is impaired in cardiovascular and chronic kidney diseases because of oxidant stress-induced nitric oxide availability reduction. The Mediterranean diet, which is characterized by food containing phenols, was correlated with a reduced incidence of cardiovascular diseases and delayed progression toward end stage chronic renal failure. Previous studies demonstrated that both red and white wine exert cardioprotective effects. In particular, wine contains Caffeic acid (CAF), an active component with known antioxidant activities., Aim of the Study: The aim of the present study was to investigate the protective effect of low doses of CAF on oxidative stress-induced endothelial injury., Results: CAF increased basal as well as acetylcholine-induced NO release by a mechanism independent from eNOS expression and phosphorylation. In addition, low doses of CAF (100 nM and 1 μM) increased proliferation and angiogenesis and inhibited leukocyte adhesion and endothelial cell apoptosis induced by hypoxia or by the uremic toxins ADMA, p-cresyl sulfate and indoxyl sulfate. The biological effects exerted by CAF on endothelial cells may be at least in part ascribed to modulation of NO release and by decreased ROS production. In an experimental model of kidney ischemia-reperfusion injury in mice, CAF significantly decreased tubular cell apoptosis, intraluminal cast deposition and leukocyte infiltration., Conclusion: The results of the present study suggest that CAF, at very low dosages similar to those observed after moderate white wine consumption, may exert a protective effect on endothelial cell function by modulating NO release independently from eNOS expression and phosphorylation. CAF-induced NO modulation may limit cardiovascular and kidney disease progression associated with oxidative stress-mediated endothelial injury.
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- 2015
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30. Endothelial progenitor cell-derived extracellular vesicles protect from complement-mediated mesangial injury in experimental anti-Thy1.1 glomerulonephritis.
- Author
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Cantaluppi V, Medica D, Mannari C, Stiaccini G, Figliolini F, Dellepiane S, Quercia AD, Migliori M, Panichi V, Giovannini L, Bruno S, Tetta C, Biancone L, and Camussi G
- Subjects
- Animals, Apoptosis, Cells, Cultured, Female, Fluorescent Antibody Technique, Glomerular Mesangium injuries, Glomerular Mesangium pathology, Glomerulonephritis pathology, Humans, RNA, Messenger genetics, Rats, Rats, Wistar, Real-Time Polymerase Chain Reaction, Reverse Transcriptase Polymerase Chain Reaction, Complement Membrane Attack Complex immunology, Endothelial Progenitor Cells immunology, Extracellular Vesicles immunology, Glomerular Mesangium immunology, Glomerulonephritis immunology, Isoantibodies immunology, Proteinuria immunology
- Abstract
Background: Endothelial progenitor cells (EPCs) are known to induce tissue repair by paracrine mechanisms including the release of growth factors and extracellular vesicles (EVs), nanoparticles able to carry proteins and genetic information to target cells. The aim of this study was to evaluate whether EVs derived from EPCs may protect from complement-mediated mesangial injury in experimental anti-Thy1.1 glomerulonephritis., Methods: EVs were isolated by serial ultracentrifugation from supernatants of cultured human EPCs and characterized for their protein and RNA content. In vivo, EVs were injected i.v. in the experimental rat model of mesangiolytic anti-Thy1.1 glomerulonephritis evaluating renal function, proteinuria, complement activity and histological lesions. In vitro, the biological effects of EPC-derived EVs were studied in cultured rat mesangial cells incubated with anti-Thy1.1 antibody and rat or human serum as complement source., Results: After i.v. injection in Thy1.1-treated rats, EVs localized within injured glomeruli and inhibited mesangial cell activation, leucocyte infiltration and apoptosis, decreased proteinuria, increased serum complement haemolytic activity (CH50) and ameliorated renal function. EV treatment decreased intraglomerular deposition of the membrane attack complex (MAC or C5b-9) and expression of smooth muscle cell actin and preserved the endothelial antigen RECA-1 and the podocyte marker synaptopodin. The protective effect of EVs was significantly reduced by pre-treatment with a high dose of RNase (1 U/mL), suggesting a key role for EV-carried RNAs in these mechanisms. Indeed, EPC-derived EVs contained different mRNAs coding for several anti-apoptotic molecules and for the complement inhibitors Factor H, CD55 and CD59 and the related proteins. The in vitro experiments aimed to investigate the mechanisms of EV protection indicated that EVs transferred to mesangial cell mRNAs coding for Factor H, CD55 and CD59 and inhibited anti-Thy1.1 antibody/complement-induced apoptosis and C5b-9/C3 mesangial cell deposition., Conclusions: EVs derived from EPCs exert a protective effect in Thy1.1 glomerulonephritis by inhibition of antibody- and complement-mediated injury of mesangial cells., (© The Author 2014. Published by Oxford University Press on behalf of ERA-EDTA. All rights reserved.)
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- 2015
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31. Microvesicles derived from endothelial progenitor cells protect the kidney from ischemia-reperfusion injury by microRNA-dependent reprogramming of resident renal cells.
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Cantaluppi V, Gatti S, Medica D, Figliolini F, Bruno S, Deregibus MC, Sordi A, Biancone L, Tetta C, and Camussi G
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- Acute Kidney Injury genetics, Acute Kidney Injury metabolism, Acute Kidney Injury pathology, Animals, Apoptosis, Capillaries metabolism, Capillaries pathology, Cell Hypoxia, Cell Proliferation, Cell-Derived Microparticles metabolism, Cell-Derived Microparticles pathology, Cells, Cultured, Chemotaxis, Leukocyte, Disease Models, Animal, Endothelial Cells metabolism, Endothelial Cells pathology, Epithelial Cells metabolism, Epithelial Cells pathology, Fibrosis, Gene Expression Regulation, Kidney blood supply, Kidney pathology, Kidney Tubules metabolism, Kidney Tubules pathology, Male, Oligonucleotides metabolism, RNA Interference, Rats, Rats, Wistar, Regeneration, Reperfusion Injury genetics, Reperfusion Injury metabolism, Reperfusion Injury pathology, Ribonuclease III genetics, Ribonuclease III metabolism, Time Factors, Transfection, Acute Kidney Injury prevention & control, Cell-Derived Microparticles transplantation, Endothelial Cells transplantation, Kidney metabolism, MicroRNAs metabolism, Reperfusion Injury prevention & control, Stem Cell Transplantation, Stem Cells metabolism, Stem Cells pathology
- Abstract
Endothelial progenitor cells are known to reverse acute kidney injury by paracrine mechanisms. We previously found that microvesicles released from these progenitor cells activate an angiogenic program in endothelial cells by horizontal mRNA transfer. Here, we tested whether these microvesicles prevent acute kidney injury in a rat model of ischemia-reperfusion injury. The RNA content of microvesicles was enriched in microRNAs (miRNAs) that modulate proliferation, angiogenesis, and apoptosis. After intravenous injection following ischemia-reperfusion, the microvesicles were localized within peritubular capillaries and tubular cells. This conferred functional and morphologic protection from acute kidney injury by enhanced tubular cell proliferation, reduced apoptosis, and leukocyte infiltration. Microvesicles also protected against progression of chronic kidney damage by inhibiting capillary rarefaction, glomerulosclerosis, and tubulointerstitial fibrosis. The renoprotective effect of microvesicles was lost after treatment with RNase, nonspecific miRNA depletion of microvesicles by Dicer knock-down in the progenitor cells, or depletion of pro-angiogenic miR-126 and miR-296 by transfection with specific miR-antagomirs. Thus, microvesicles derived from endothelial progenitor cells protect the kidney from ischemic acute injury by delivering their RNA content, the miRNA cargo of which contributes to reprogramming hypoxic resident renal cells to a regenerative program.
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- 2012
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32. Brain and muscle energy metabolism studied in vivo by 31P-magnetic resonance spectroscopy in NARP syndrome.
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Lodi R, Montagna P, Iotti S, Zaniol P, Barboni P, Puddu P, and Barbiroli B
- Subjects
- Adenosine Diphosphate analysis, Adolescent, Adult, Ataxia complications, Ataxia genetics, Brain Chemistry, Case-Control Studies, Female, Follow-Up Studies, Humans, Intellectual Disability complications, Intellectual Disability genetics, Muscles chemistry, Pedigree, Phosphocreatine analysis, Phosphorylation, Point Mutation genetics, Retinitis Pigmentosa complications, Retinitis Pigmentosa genetics, Syndrome, Ataxia metabolism, Brain metabolism, DNA, Mitochondrial genetics, Energy Metabolism, Intellectual Disability metabolism, Magnetic Resonance Spectroscopy, Muscles metabolism, Phosphorus Isotopes, Retinitis Pigmentosa metabolism
- Abstract
Phosphorus magnetic resonance spectroscopy (31P-MRS) was used to study in vivo the energy metabolism of brain and skeletal muscle in two members of an Italian pedigree with NARP syndrome due to a point mutation at bp 8993 of mtDNA. In the youngest patient, a 13 year old girl with retinitis pigmentosa, ataxia, and psychomotor retardation, there was an alteration of brain energy metabolism shown by a decreased phosphocreatine content, increased [ADP] and decreased phosphorylation potential. The energy metabolism of her skeletal muscle was also abnormal, as shown by resting higher inorganic phosphate and lower phosphocreatine concentrations than in normal subjects. Her mother, a 41 year old woman with minimal clinical involvement, showed a milder derangement of brain energy metabolism and normal skeletal muscle. Findings with MRS showed that this point mutation of mtDNA is responsible for a derangement of energy metabolism in skeletal muscle and even more so in the brain.
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- 1994
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