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2 results on '"Milieu INRA96"'

1. Freezing stallion semen in INRA96®-based extender improves fertility rates in comparison with INRA82

Author

Pillet, Elodie, Batellier, Florence, Duchamp, Guy, Furstoss, Vincent, Le Vern, Yves, Kerboeuf, Dominique, Vidament, Marianne, Magistrini, Michèle, Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS), IMV Technologies, Unité Pluri-espèces d'Expérimentation Animale en Physiologie de la Reproduction et des Comportements (TOURS UPEA PRC), Institut National de la Recherche Agronomique (INRA), Insémination Caprine et Porcine (ICP), and Infectiologie Animale et Santé Publique (UR IASP)

Subjects
Cryoconservation, [SDV.SA]Life Sciences [q-bio]/Agricultural sciences, Stallion, Fertility, Semen, Etalon, Milieu INRA96
Abstract

International audience; The chemical composition of freezing extenders plays a major role in sperm cell survival during cryopreservation. In this study we compared two extenders for freezing stallion semen: INRA82 extender (as a control) versus INRA96 (R) extender, both supplemented with egg yolk and glycerol. INRA82 contains milk, whereas INRA96 (R) is a chemically- defined extender developed for fresh semen storage at 4 degrees C or 15 degrees C. Semen from 3 stallions (7 ejaculates per stallion) was frozen in both extenders. In vitro analyses of post-thaw motility of sperm cells (computer-assisted analysis) and of membrane integrity (flow cytometry analysis) were performed. Then a fertility trial was conducted. Inseminations were conducted in a total of 84 mare cycles. INRA96 (R) extender supplemented with egg yolk and glycerol significantly improved per-cycle pregnancy rates compared with INRA82 (71% versus 40%, p < 0.01). In agreement with these fertility results, membrane integrity was better preserved in INRA96 (R) than in INRA82. In contrast, motility parameters were significantly higher in INRA82 than in INRA96 (R). Further research is needed to understand how INRA96 (R) components protect sperm cells during the cryopreservation process and highly increase their fertility potential compared with INRA82 components.

Published
2008
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2. La congélation de semence d'étalon dans un milieu à base d'INRA96® améliore le taux de fertilité en comparaison avec le milieu INRA82

Author

V. Furstoss, Florence Batellier, Elodie Pillet, Yves Le Vern, Dominique Kerboeuf, Guy Duchamp, Michèle Magistrini, Marianne Vidament, Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), IMV Technologies, Unité Pluri-espèces d'Expérimentation Animale en Physiologie de la Reproduction et des Comportements (TOURS UPEA PRC), Institut National de la Recherche Agronomique (INRA), Insémination Caprine et Porcine (ICP), and Infectiologie Animale et Santé Publique (UR IASP)

Subjects
Cryoconservation, [SDV.SA]Life Sciences [q-bio]/Agricultural sciences, food.ingredient, Etalon, Total fertility rate, media_common.quotation_subject, reproduction animale, Semen, Fertility, Milieu INRA96, Biochemistry, Cryopreservation, law.invention, Andrology, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, food, milieu de conservation, law, Yolk, Glycerol, conservation du sperme, media_common, Stallion, 030219 obstetrics & reproductive medicine, Chemistry, Extender, 0402 animal and dairy science, fertilité animale, 04 agricultural and veterinary sciences, 040201 dairy & animal science, Sperm, Agricultural sciences, cheval, semence, Sciences agricoles, Food Science, équin
Abstract

International audience; The chemical composition of freezing extenders plays a major role in sperm cell survival during cryopreservation. In this study we compared two extenders for freezing stallion semen: INRA82 extender (as a control) versus INRA96 (R) extender, both supplemented with egg yolk and glycerol. INRA82 contains milk, whereas INRA96 (R) is a chemically- defined extender developed for fresh semen storage at 4 degrees C or 15 degrees C. Semen from 3 stallions (7 ejaculates per stallion) was frozen in both extenders. In vitro analyses of post-thaw motility of sperm cells (computer-assisted analysis) and of membrane integrity (flow cytometry analysis) were performed. Then a fertility trial was conducted. Inseminations were conducted in a total of 84 mare cycles. INRA96 (R) extender supplemented with egg yolk and glycerol significantly improved per-cycle pregnancy rates compared with INRA82 (71% versus 40%, p < 0.01). In agreement with these fertility results, membrane integrity was better preserved in INRA96 (R) than in INRA82. In contrast, motility parameters were significantly higher in INRA82 than in INRA96 (R). Further research is needed to understand how INRA96 (R) components protect sperm cells during the cryopreservation process and highly increase their fertility potential compared with INRA82 components.

Published
2008
Full Text
View/download PDF

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