42 results on '"Mira, Sara"'
Search Results
2. Seed Longevity and Ageing: A Review on Physiological and Genetic Factors with an Emphasis on Hormonal Regulation
- Author
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Agencia Estatal de Investigación (España), Ministerio de Ciencia e Innovación (España), Universidad Politécnica de Madrid, Ministerio de Ciencia, Innovación y Universidades (España), Pirredda, Michela [0000-0002-8223-8260], Fañanás-Pueyo, Iris [0000-0001-7608-1538], Oñate-Sánchez, Luis [0000-0001-7047-5739], Mira, Sara [0000-0002-0164-5156], Pirredda, Michela, Fañanás-Pueyo, Iris, Oñate-Sánchez, Luis, Mira, Sara, Agencia Estatal de Investigación (España), Ministerio de Ciencia e Innovación (España), Universidad Politécnica de Madrid, Ministerio de Ciencia, Innovación y Universidades (España), Pirredda, Michela [0000-0002-8223-8260], Fañanás-Pueyo, Iris [0000-0001-7608-1538], Oñate-Sánchez, Luis [0000-0001-7047-5739], Mira, Sara [0000-0002-0164-5156], Pirredda, Michela, Fañanás-Pueyo, Iris, Oñate-Sánchez, Luis, and Mira, Sara
- Abstract
Upon storage, seeds inevitably age and lose their viability over time, which determines their longevity. Longevity correlates with successful seed germination and enhancing this trait is of fundamental importance for long-term seed storage (germplasm conservation) and crop improvement. Seed longevity is governed by a complex interplay between genetic factors and environmental conditions experienced during seed development and after-ripening that will shape seed physiology. Several factors have been associated with seed ageing such as oxidative stress responses, DNA repair enzymes, and composition of seed layers. Phytohormones, mainly abscisic acid, auxins, and gibberellins, have also emerged as prominent endogenous regulators of seed longevity, and their study has provided new regulators of longevity. Gaining a thorough understanding of how hormonal signalling genes and pathways are integrated with downstream mechanisms related to seed longevity is essential for formulating strategies aimed at preserving seed quality and viability. A relevant aspect related to research in seed longevity is the existence of significant differences between results depending on the seed equilibrium relative humidity conditions used to study seed ageing. Hence, this review delves into the genetic, environmental and experimental factors affecting seed ageing and longevity, with a particular focus on their hormonal regulation. We also provide gene network models underlying hormone signalling aimed to help visualize their integration into seed longevity and ageing. We believe that the format used to present the information bolsters its value as a resource to support seed longevity research for seed conservation and crop improvement.
- Published
- 2024
3. Complex control of seed germination timing by ERF50 involves RGL2 antagonism and negative feedback regulation of DOG1
- Author
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Agencia Estatal de Investigación (España), Ministerio de Ciencia e Innovación (España), European Commission, Universidad Politécnica de Madrid, Comunidad de Madrid, Carrera-Castaño, Gerardo [0000-0002-0393-3186], Mira, Sara [0000-0002-0164-5156], Fañanás-Pueyo, Iris [0000-0001-7608-1538], Sánchez, Rocío [0009-0000-5805-3496], Contreras, Ángela [0000-0003-3780-8428], Weiste, Christoph [0000-0003-1894-7924], Dröge-Laser, Wolfgang [0000-0003-4066-8024], Plaza Gómez, Luis Manuel [0000-0002-4346-4820], Oñate-Sánchez, Luis [0000-0001-7047-5739], Carrera-Castaño, Gerardo, Mira, Sara, Fañanás-Pueyo, Iris, Sánchez, Rocío, Contreras, Ángela, Weiste, Christoph, Dröge-Laser, Wolfgang, Plaza Gómez, Luis Manuel, Oñate-Sánchez, Luis, Agencia Estatal de Investigación (España), Ministerio de Ciencia e Innovación (España), European Commission, Universidad Politécnica de Madrid, Comunidad de Madrid, Carrera-Castaño, Gerardo [0000-0002-0393-3186], Mira, Sara [0000-0002-0164-5156], Fañanás-Pueyo, Iris [0000-0001-7608-1538], Sánchez, Rocío [0009-0000-5805-3496], Contreras, Ángela [0000-0003-3780-8428], Weiste, Christoph [0000-0003-1894-7924], Dröge-Laser, Wolfgang [0000-0003-4066-8024], Plaza Gómez, Luis Manuel [0000-0002-4346-4820], Oñate-Sánchez, Luis [0000-0001-7047-5739], Carrera-Castaño, Gerardo, Mira, Sara, Fañanás-Pueyo, Iris, Sánchez, Rocío, Contreras, Ángela, Weiste, Christoph, Dröge-Laser, Wolfgang, Plaza Gómez, Luis Manuel, and Oñate-Sánchez, Luis
- Abstract
Seed dormancy governs germination timing, with both evolutionary and applied consequences. Despite extensive studies on the hormonal and genetic control of these processes, molecular mechanisms directly linking dormancy and germination remain poorly understood. By screening a collection of lines overexpressing Arabidopsis transcription factors, we identified ERF50 as a key gene to control dormancy and germination. To study its regulation, we measured seed-related physiological parameters in loss-of-function mutants and carried out transactivation, protein interaction and ChIP-PCR analyses. We found direct ERF50-mediated repression of DOG1 and activation of EXPA2 transcription, which results in enhanced seed germination. Although ERF50 expression is increased by DOG1 in dormant seeds, ERF50 germination-promoting activity is blocked by RGL2. The physiological, genetic and molecular evidence gathered here supports that ERF50 controls germination timing by regulating DOG1 levels to leverage its role as enhancer of seed germination, via RGL2 antagonism on EXPA2 expression. Our results highlight the central role of ERF50 as a feedback regulator to couple and fine-tune seed dormancy and germination.
- Published
- 2024
4. Zn Oxide Nanoparticles and Fine Particles: Synthesis, Characterization and Evaluation of the Toxic Effect on Germination and Vigour of Solanum licopersicum L.
- Author
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de Francisco, Marina, primary, Mira, Sara, additional, Durães, Luisa, additional, Romeiro, Andreia, additional, Álvarez-Torrellas, Silvia, additional, and Almendros, Patricia, additional
- Published
- 2024
- Full Text
- View/download PDF
5. Complex control of seed germination timing by ERF50 involves RGL2 antagonism and negative feedback regulation of DOG1
- Author
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Carrera‐Castaño, Gerardo, primary, Mira, Sara, additional, Fañanás‐Pueyo, Iris, additional, Sánchez‐Montesino, Rocío, additional, Contreras, Ángela, additional, Weiste, Christoph, additional, Dröge‐Laser, Wolfgang, additional, Gómez, Luis, additional, and Oñate‐Sánchez, Luis, additional
- Published
- 2024
- Full Text
- View/download PDF
6. Seed Longevity and Ageing: A Review on Physiological and Genetic Factors with an Emphasis on Hormonal Regulation
- Author
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Pirredda, Michela, primary, Fañanás-Pueyo, Iris, additional, Oñate-Sánchez, Luis, additional, and Mira, Sara, additional
- Published
- 2023
- Full Text
- View/download PDF
7. Turismo científico em regiões turísticas de sol e mar: Contributos para um roteiro metodológico de investigação na região do Algarve (portugal)
- Author
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Nunes Henriques, Clàudia Helena, Afonso, Carlos, Rodrigues, Luís Azevedo, Varela, Maria de Lurdes Figueirinha, Mira, Sara, Nunes Henriques, Clàudia Helena, Afonso, Carlos, Rodrigues, Luís Azevedo, Varela, Maria de Lurdes Figueirinha, and Mira, Sara
- Abstract
This article presents a methodological roadmap for identifying tourist-scientific resources in the Algarve. With ten phases, it spans from conceptualization to the classification of scientific domains, aiming to promote tourism in the municipalities of Monchique, Lagos, Aljezur, and Vila do Bispo, both domestically and internationally. The stages include sustainable tourism policy, territorialization, creation of a theoretical model for inventorying cultural resources, tourist experiences/products, destination governance/management, and information collection methods. This roadmap seeks to provide a comprehensive approach to boost tourism in the region, emphasizing its scientific and touristic significance., Este artigo apresenta um roteiro metodológico para identificar recursos turísticocientíficos no Algarve. Com dez fases, abrange desde a conceptualização até a classificação de domínios científicos, visando promover o turismo nos concelhos de Monchique, Lagos, Aljezur e Vila do Bispo, tanto a nível doméstico quanto internacional. As etapas incluem a política de turismo sustentável, territorialização, criação de modelo teórico para inventariação de recursos culturais, experiências/ produtos turísticos, governança/gestão do destino e métodos de recolha de informação. Este roteiro busca oferecer uma abordagem abrangente para impulsionar o turismo na região, destacando sua relevância científica e turística
- Published
- 2023
8. Seed Longevity and Ageing: A Review on Physiological and Genetic Factors with an Emphasis on Hormonal Regulation.
- Author
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Pirredda, Michela, Fañanás-Pueyo, Iris, Oñate-Sánchez, Luis, and Mira, Sara
- Subjects
LONGEVITY ,SEED viability ,PLANT hormones ,SEED storage ,DNA ligases ,AGE ,COMPOSITION of seeds - Abstract
Upon storage, seeds inevitably age and lose their viability over time, which determines their longevity. Longevity correlates with successful seed germination and enhancing this trait is of fundamental importance for long-term seed storage (germplasm conservation) and crop improvement. Seed longevity is governed by a complex interplay between genetic factors and environmental conditions experienced during seed development and after-ripening that will shape seed physiology. Several factors have been associated with seed ageing such as oxidative stress responses, DNA repair enzymes, and composition of seed layers. Phytohormones, mainly abscisic acid, auxins, and gibberellins, have also emerged as prominent endogenous regulators of seed longevity, and their study has provided new regulators of longevity. Gaining a thorough understanding of how hormonal signalling genes and pathways are integrated with downstream mechanisms related to seed longevity is essential for formulating strategies aimed at preserving seed quality and viability. A relevant aspect related to research in seed longevity is the existence of significant differences between results depending on the seed equilibrium relative humidity conditions used to study seed ageing. Hence, this review delves into the genetic, environmental and experimental factors affecting seed ageing and longevity, with a particular focus on their hormonal regulation. We also provide gene network models underlying hormone signalling aimed to help visualize their integration into seed longevity and ageing. We believe that the format used to present the information bolsters its value as a resource to support seed longevity research for seed conservation and crop improvement. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
9. Volatile emission in dry seeds as a way to probe chemical reactions during initial asymptomatic deterioration
- Author
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Mira, Sara, Hill, Lisa M., González-Benito, M. Elena, Ibáñez, Miguel Angel, and Walters, Christina
- Published
- 2016
10. Characterization of volatile production during storage of lettuce (Lactuca sativa) seed
- Author
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Mira, Sara, González-Benito, M. Elena, Hill, Lisa M., and Walters, Christina
- Published
- 2010
11. Programa de intervención para la mejora de habilidades sociales en niños y niñas con altas capacidades
- Author
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González Mira, Sara, Soto Sanz, Victoria, and Departamentos de la UMH::Psicología de la Salud
- Subjects
desarrollo asincrónico ,high abilities ,social skills ,habilidades sociales ,1 - Filosofía y psicología::159.9 - Psicología [CDU] ,primera infancia ,programa de intervención ,asynchronous development ,altas capacidades ,early childhood ,intervention programme - Abstract
Los niños con Altas Capacidades (AACC) presentan un desarrollo asincrónico, en el ámbito intelectual se encuentran más desarrollados que la media, sin embargo, presentan déficits en las Habilidades Sociales (HHSS). El presente trabajo tiene como objetivo general diseñar un programa de intervención para promover la mejora de las habilidades sociales en niños de 6 a 8 años con diagnóstico de Altas Capacidades pertenecientes a la provincia de Alicante. Con esta finalidad, el presente proyecto se compone de 16 sesiones donde se realizarán actividades para la psicoeducación, regulación emocional, empatía, normas sociales, manejo de la ansiedad, asertividad, habilidad para la resolución de conflictos y autoestima. Se prevé que la implementación de un programa de este tipo consiga mejorar las habilidades sociales de dicho colectivo. Se considera relevante la aplicación y la comprobación de la eficacia del presente programa, para conseguir una mejora en las HHSS y prevenir posibles problemas evolutivos en niños con AACC que se encuentran en la primera infancia. Children with High Abilities (AACC) present an asynchronous development, in the intellectual area they are more developed than the average, however, they present deficits in Social Skills (HHSS). The general objective of this work is to design an intervention programme to promote the improvement of social skills in children aged 6 to 8 years with a diagnosis of High Abilities in the province of Alicante. To this end, this project consists of 16 sessions where activities for psychoeducation, emotional regulation, empathy, social norms, anxiety management, assertiveness, conflict resolution skills and self-esteem will be carried out. The implementation of such a programme is expected to improve the social skills of this group. The application and verification of the effectiveness of this programme is considered relevant to achieve an improvement in HHSS and prevent possible developmental problems in children with AACC who are in early childhood.
- Published
- 2022
12. The contribute of Occupational Therapy to the implementation of Augmentative and Alternative Communication Systems in children with Cerebral Palsy: a scoping review
- Author
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Duarte, Francisca, primary, Leal, Gabriela, additional, Mira, Sara, additional, Ribeiro, Sofia, additional, and Ribeiro, Jaime, additional
- Published
- 2022
- Full Text
- View/download PDF
13. Antioxidant, Mineralogenic and Osteogenic Activities of Spartina alterniflora and Salicornia fragilis Extracts Rich in Polyphenols
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Roberto, Vânia P., primary, Surget, Gwladys, additional, Le Lann, Klervi, additional, Mira, Sara, additional, Tarasco, Marco, additional, Guérard, Fabienne, additional, Poupart, Nathalie, additional, Laizé, Vincent, additional, Stiger-Pouvreau, Valérie, additional, and Cancela, M. Leonor, additional
- Published
- 2021
- Full Text
- View/download PDF
14. 'HISTORIAS ILUSTRADAS DE MI ABUELO'EL DIBUJO EXPRESIVO COMO LENGUAJE ARTÍSTICO EN LA ILUSTRACIÓN
- Author
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Gozalbo Mira, Sara
- Subjects
Història ,Illustration ,History ,DIBUJO ,Book ,Grado en Bellas Artes-Grau en Belles Arts ,Il·lustració ,Dibuix expressiu ,Grandfather ,Llibre ,Avi ,Expressive drawing - Abstract
[CA] Aquest treball és una recopilació d¿històries que el meu avi m¿ha contat sobre el seu passat i el seu entorn. Cadascuna de les històries del llibre va acompanyada d¿una il·lustració elaborada mitjançant la tècnica del dibuix expressiu representant la vitalitat de l¿ésser i el seu caràcter. A aquestes històries es dóna a conèixer la forma de vida des de l¿any 1936, data del seu naixement, fins a hui. Mitjançant anècdotes del meu avi i la seua família, podem trobar des d¿històries amb el seu pare i les seues aventures per a sobreviure, fins a moments del meu germà, acompanyat del nostre avi. Cada capítol tracta una època de la seua vida o d¿algun dels seus familiars propers. Així, podem vorer el recorregut de la seua història des d¿abans de nàixer fins ara, als seus 83 anys. El llibre consta d¿un pròleg, huit capítols i una conclusió., [EN] This final grade project is a compilation of stories which were told to me by my grandfather. Those stories are about his past and his surroundings. Each story of the book is accompanied of an illustration which is done by expressive drawing techniques and by the vitality and kind of the being. In these stories, lifestyle from 1936 ,his birth year, is shown til the present day. Trough my grandfather¿s and his family¿s memories, we can see anecdotes of his father¿s surviving adventures (before 1936) until my brother¿s memories with our grandfather. Each chapter deals with a different time of his life or his closest family. Thus, we can see, his historical route before he was born, til now at his 83 years old. The book has a prologue, made by eight chapters and a conclusion.
- Published
- 2020
15. Antioxidant, Mineralogenic and Osteogenic Activities of Spartina alterniflora and Salicornia fragilis Extracts Rich in Polyphenols
- Author
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Roberto, Vânia P., Surget, Gwladys, Le Lann, Klervi, Mira, Sara, Tarasco, Marco, Guérard, Fabienne, Poupart, Nathalie, Laizé, Vincent, Stiger-pouvreau, Valerie, Cancela, M. Leonor, Roberto, Vânia P., Surget, Gwladys, Le Lann, Klervi, Mira, Sara, Tarasco, Marco, Guérard, Fabienne, Poupart, Nathalie, Laizé, Vincent, Stiger-pouvreau, Valerie, and Cancela, M. Leonor
- Abstract
Osteoporosis is an aging-related disease and a worldwide health issue. Current therapeutics have failed to reduce the prevalence of osteoporosis in the human population, thus the discovery of compounds with bone anabolic properties that could be the basis of next generation drugs is a priority. Marine plants contain a wide range of bioactive compounds and the presence of osteoactive phytochemicals was investigated in two halophytes collected in Brittany (France): the invasive Spartina alterniflora and the native Salicornia fragilis. Two semi-purified fractions, prepared through liquid-liquid extraction, were assessed for phenolic and flavonoid contents, and for the presence of antioxidant, mineralogenic and osteogenic bioactivities. Ethyl acetate fraction (EAF) was rich in phenolic compounds and exhibited the highest antioxidant activity. While S. fragilis EAF only triggered a weak proliferative effect in vitro, S. alterniflora EAF potently induced extracellular matrix mineralization (7-fold at 250 μg/mL). A strong osteogenic effect was also observed in vivo using zebrafish operculum assay (2.5-fold at 10 μg/mL in 9-dpf larvae). Results indicate that polyphenol rich EAF of S. alterniflora has both antioxidant and bone anabolic activities. As an invasive species, this marine plant may represent a sustainable source of molecules for therapeutic applications in bone disorders.
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- 2021
- Full Text
- View/download PDF
16. Application of the MSAP Technique to Evaluate Epigenetic Changes in Plant Conservation
- Author
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González-Benito, María Elena, primary, Ibáñez, Miguel Ángel, additional, Pirredda, Michela, additional, Mira, Sara, additional, and Martín, Carmen, additional
- Published
- 2020
- Full Text
- View/download PDF
17. Neutrophil and Platelet to Lymphocyte Ratio for Detecting Early-onset Neonatal Sepsis
- Author
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mira, sara, primary, Alkhalegy, Hany, additional, Ibrahim, Sabah, additional, and elbakry, elbakry, additional
- Published
- 2020
- Full Text
- View/download PDF
18. Genetic and Epigenetic Stability in Rye Seeds under Different Storage Conditions: Ageing and Oxygen Effect
- Author
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Pirredda, Michela, primary, González-Benito, M. Elena, additional, Martín, Carmen, additional, and Mira, Sara, additional
- Published
- 2020
- Full Text
- View/download PDF
19. O contributo da Terapia Ocupacional para a implementação de Sistemas Aumentativos e Alternativos de Comunicação em crianças com Paralisia Cerebral: uma scoping review.
- Author
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Duarte, Francisca, Leal, Gabriela, Mira, Sara, Ribeiro, Sofia, and Ribeiro, Jaime
- Published
- 2022
20. Projeto A Minha Praia: A Ciência Cidadã enquanto promotora do civismo ambiental e conservação da Natureza
- Author
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Gouveia, Licínia, Lourenço, Carla, Afonso, João, Freitas, Ricardo, Cavaquinho, Vanessa, Fernandes, Aurélie, Domingues, Pedro, Veiga-Pires, Cristina, Reis, Emanuel, Rodrigues, Miguel, Gomes, Tiago, Rodrigues, Luís, Mira, Sara, Ferreira, Hélder, Pinto, Andreia, Vaz, Paula, Drago, Teresa, Soares, Isabel, and Ramos, Ana
- Abstract
À escala global, a conjugação das atividades humanas com o rápido incremento da população e os sucessivos avanços tecnológicos exercem uma crescente pressão sobre os ambientes marinhos. Atualmente, o lixo marinho é uma das maiores ameaças à vitalidade do Oceano Global: leva à destruição de habitats, à mortalidade de várias espécies, a efeitos adversos na nossa saúde e à quebra geral da produtividade económica providenciada pelo mar. Foi tendo em conta a magnitude e as consequências da disseminação do lixo marinho e a exposição que a região do Algarve tem ao problema, que o Centro Ciência Viva de Tavira assumiu a coordenação do Projeto A Minha Praia, entre Agosto de 2018 e Julho de 2019: um projeto de monitorização ambiental e de sensibilização para a conservação do meio marinho e promoção de hábitos de consumo sustentáveis, através de ações de civismo ambiental e de ciência cidadã. O Projeto A Minha Praia foi um dos vencedores da primeira edição do Orçamento Participativo Portugal (OPP) em 2017, e a sua execução foi garantida pela colaboração entre os três Centros Ciência Viva (CCVs) da região, com o envolvimento de várias entidades regionais e nacionais. Com este projeto, criou-se uma rede de monitorização do lixo marinho em seis praias do Algarve, na qual o público escolar e as camadas mais jovens da região realizaram ações periódicas de sensibilização para a problemática do lixo marinho, sua origem e alcance, bem como a sua catalogação e recolha. O lixo recolhido foi triado e classificado de acordo com a metodologia OSPAR, produzindo informação que foi entregue à Agência Portuguesa do Ambiente, a entidade responsável pela monitorização do lixo marinho em Portugal. O Projeto A Minha Praia também apresenta uma vertente técnica, já que nos CCVs aproveitamos para demonstrar que o plástico, que é o maior componente do lixo encontrado no mar e zonas costeiras, deve ser encarado como um matéria-prima a ser aproveitada, reciclada e transformada em novos objetos de carácter prático ou artístico que prolongam a vida útil deste material., Concretização deste poster no âmbito do projecto OPP-A Minha Praia (OPP220/437), financiado pela Ciência Viva - Agência Nacional para a Cultura Científica e Tecnológica e pela FCT - Fundação para a Ciência e Tecnologia
- Published
- 2019
21. Um ano de escola n'A Minha Praia – sensibilização para a problemática do lixo marinho utilizando a ciência-cidadã como ferramenta
- Author
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Gouveia, Licínia, Lourenço, Carla, Afonso, João, Freitas, Ricardo, Cavaquinho, Vanessa, Fernandes, Aurélie, Domingues, Pedro, Veiga-Pires, Cristina, Reis, Emanuel, Rodrigues, Miguel, Gomes, Tiago, Rodrigues, Luís, Mira, Sara, Ferreira, Hélder, Pinto, Andreia, Vaz, Paula, Drago, Teresa, Soares, Isabel, and Ramos, Ana A.
- Subjects
Ciência cidadã ,OPP- A Minha Praia, Lixo marinho, ciência cidadã ,Lixo marinho ,OPP- A Minha Praia - Abstract
Um ano de escola n’A Minha Praia – sensibilização para a problemática do lixo marinho utilizando a ciência-cidadã como ferramenta O Algarve reúne um conjunto de características que o tornam particularmente vulnerável à presença de lixo marinho e aos seus efeitos nefastos: uma orla costeira longa (aprox. 200km), um destino turístico muito popular, uma população residente concentrada no litoral e uma fração significativa da sociedade cuja subsistência depende de atividades económicas ligadas ao mar (pesca, marisqueio, extração de sal, passeios turísticos, etc.). Foi tendo em conta a magnitude e as consequências da propagação do lixo marinho na região que o Centro Ciência Viva de Tavira coordenou o projeto A Minha Praia, um dos vencedores da primeira edição do Orçamento Participativo Portugal (OPP) em 2017, e cuja execução foi garantida pela colaboração entre os três Centros Ciência Viva (CCVs) no Algarve, com o envolvimento de várias entidades regionais e nacionais. Este projeto permitiu que cerca de 933 alunos provenientes de 17 escolas fossem sensibilizados para a conservação do meio marinho, para hábitos de consumo sustentáveis e para o civismo ambiental, em simultâneo com ações de ciência-cidadã, nas quais periodicamente monitorizavam a presença de lixo marinho em seis praias da costa sul do Algarve. Ainda dentro da função didática do projeto, demonstramos a valorização do plástico (que é o maior componente do lixo marinho), aproveitando-o, reciclando-o e transformando-o em novos objetos que prolongam a sua utilidade., Esta apresentação foi dinamizada no âmbito do projecto OPP-A Minha (OPP220/437), financiado pela Ciência Viva - Agência Nacional para a Cultura Científica e Tecnológica e pela FCT - Fundação para a Ciência e Tecnologia.
- Published
- 2019
22. A ciência-cidadã ao serviço da mudança: o exemplo do projeto A Minha Praia
- Author
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Gouveia, Licínia, Lourenço, Carla, Afonso, João, Freitas, Ricardo, Veiga-Pires, Cristina, Reis, Emanuel, Rodrigues, Miguel, Gomes, Tiago, Rodrigues, Luís, Mira, Sara, Ferreira, Hélder, Pinto, Andreia, Vaz, Paula, Drago, Teresa, Soares, Isabel, and Ramos, Ana
- Abstract
Vencedor da 1ª edição do Orçamento Participativo Portugal (2017), A Minha Praia é um projeto de monitorização ambiental que pretende sensibilizar para a conservação do meio marinho e estimular hábitos de consumo responsáveis, através de ações de ciência cidadã e civismo ambiental., SFRH/BGCT/135195/2017
- Published
- 2019
- Full Text
- View/download PDF
23. 'HISTORIAS ILUSTRADAS DE MI ABUELO'EL DIBUJO EXPRESIVO COMO LENGUAJE ARTÍSTICO EN LA ILUSTRACIÓN
- Author
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Rodríguez León, Alejandro, Universitat Politècnica de València. Departamento de Dibujo - Departament de Dibuix, Universitat Politècnica de València. Facultad de Bellas Artes - Facultat de Belles Arts, Gozalbo Mira, Sara, Rodríguez León, Alejandro, Universitat Politècnica de València. Departamento de Dibujo - Departament de Dibuix, Universitat Politècnica de València. Facultad de Bellas Artes - Facultat de Belles Arts, and Gozalbo Mira, Sara
- Abstract
[CA] Aquest treball és una recopilació d¿històries que el meu avi m¿ha contat sobre el seu passat i el seu entorn. Cadascuna de les històries del llibre va acompanyada d¿una il·lustració elaborada mitjançant la tècnica del dibuix expressiu representant la vitalitat de l¿ésser i el seu caràcter. A aquestes històries es dóna a conèixer la forma de vida des de l¿any 1936, data del seu naixement, fins a hui. Mitjançant anècdotes del meu avi i la seua família, podem trobar des d¿històries amb el seu pare i les seues aventures per a sobreviure, fins a moments del meu germà, acompanyat del nostre avi. Cada capítol tracta una època de la seua vida o d¿algun dels seus familiars propers. Així, podem vorer el recorregut de la seua història des d¿abans de nàixer fins ara, als seus 83 anys. El llibre consta d¿un pròleg, huit capítols i una conclusió., [EN] This final grade project is a compilation of stories which were told to me by my grandfather. Those stories are about his past and his surroundings. Each story of the book is accompanied of an illustration which is done by expressive drawing techniques and by the vitality and kind of the being. In these stories, lifestyle from 1936 ,his birth year, is shown til the present day. Trough my grandfather¿s and his family¿s memories, we can see anecdotes of his father¿s surviving adventures (before 1936) until my brother¿s memories with our grandfather. Each chapter deals with a different time of his life or his closest family. Thus, we can see, his historical route before he was born, til now at his 83 years old. The book has a prologue, made by eight chapters and a conclusion.
- Published
- 2020
24. Projecto 'A minha praia': cidadania ambiental para sensibilização, monitorização e limpeza do lixo marinho na região do Algarve
- Author
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Ramos, Ana A, Lourenço, Carla, Afonso, João, Freitas, Ricardo, Veiga-Pires, Cristina, Reis, Emanuel, Rodrigues, Miguel, Gomes, Tiago, Rodrigues, Luís, Mira, Sara, Leote, Catarina, Ferreira, Hélder, Pinto, Andreia, Vaz, Paula, Drago, Teresa, and Borges, Rita
- Subjects
OPP 2017 ,Poluição por plásticos ,OPP 2017, A minha praia, lixo marinho, poluição por plásticos ,Lixo marinho ,A minha praia - Abstract
O projecto “A minha praia”, um dos vencedores do Orçamento Participativo OPP – Ciência 2017 pretende contribuir para a conservação do meio marinho e desenvolvimento sustentável com a criação de uma rede alargada de monitorização de lixo marinho ao longo da costa Algarvia. Através da cooperação de diferentes intervenientes (Centros Ciência Viva, comunidade escolar, Investigadores, municípios e entidades regionais) serão promovidas acções de ciência cidadã de: 1) limpeza e monitorização do lixo marinho de seis praias referenciadas da região; 2) actividades educativas de reutilização e reciclagem de materiais de plástico recolhidos em estações de reciclagem implementadas nos Centros Ciência Viva. Para além da obtenção de informação relevante para avaliação do lixo marinho encontrado nas praias (e.g. origem, quantidade, variação anual) este projecto irá contribuir para a sensibilização da sociedade e em particular da comunidade escolar para a problemática e impacto da poluição do meio marinho, nomeadamente por plásticos, nos ecossistemas e nos serviços por eles fornecidos e para a necessidade de um consumo sustentável e preservação dos Oceanos. Adicionalmente, contribuirá para a aproximação entre os cidadãos e a comunidade científica., Seminário Coastwatch 2018 – "Deixa a tua praia respirar" | 12 e 13 de Julho | Ericeira Painel 5 - Ciência cidadã e protecção do litoral
- Published
- 2018
- Full Text
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25. Inter-population variation in germination characteristics of 'Plantago lanceolata' seeds: effects of temperature, osmotic stress and salinity
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Mira, Sara, Veiga-Barbosa, Luciana, González-Benito, M. Elena, Pérez-García, Félix, Mira, Sara, Veiga-Barbosa, Luciana, González-Benito, M. Elena, and Pérez-García, Félix
- Abstract
Plantago lanceolata is a perennial herbaceous plant widely distributed throughout the Mediterranean region. Variation in germination requirements and tolerance to osmotic stress and salinity were tested using seeds from two wild populations. Germination experiments were conducted under controlled conditions at constant (15, 20 and 25ºC) and alternating (20/10 and 25/15ºC) temperatures, and under a 16 h/8 h light/dark photoperiod. Seeds of both populations were non-dormant and incubation temperature significantly affected the final germination percentage and germination rate. Germination percentages ranged from 27 to 85% for the different temperatures and populations. Significant inter-population variation was found for the germination percentages reached at alternate temperature regimes. To determine the effect of osmotic stress and salinity, four polyethylene glycol (PEG 6000: 10, 20, 30 and 40% w/v) and four NaCl concentrations (50, 100, 200 and 300 mmol·L-1) were tested. Seed germination significantly decreased with increasing in PEG and NaCl concentration. Seed germination recovery when they were transferred to distilled water after PEG or salinity treatments was variable depending on population, suggesting that P. lanceolata seeds are moderately tolerant to osmotic and salt stresses at germination stage., Plantago lanceolata es una planta herbácea perenne ampliamente distribuida por toda la región Mediterránea. Se estudió la variación en los requerimientos germinativos y la tolerancia al estrés osmótico y la salinidad de las semillas de dos poblaciones silvestres. Los ensayos de germinación se realizaron en condiciones controladas, a temperaturas constantes (15, 20 y 25 ºC) y alternas (20/10 y 25/15 ºC) y bajo un fotoperiodo de 16 h/8 h luz/oscuridad. Las semillas de ambas poblaciones fueron no durmientes y la temperatura de incubación afectó significativamente al porcentaje final de germinación y a la velocidad de germinación. Los porcentajes de germinación variaron entre un 27 y un 85%, dependiendo de la temperatura y población. Se encontró una variación interpoblacional significativa para los porcentajes de germinación alcanzados a los regimenes de temperaturas alternas. Para determinar el efecto del estrés osmótico y de la salinidad, se ensayaron cuatro concentraciones de polietilenglicol (PEG 6000: 10, 20, 30 y 40% w/v) y de NaCl (50, 100, 200 y 300 mmol L-1). La germinación de las semillas disminuyó significativamente con el incremento de la concentración de PEG y ClNa. La recuperación de la germinación cuando las semillas se transfirieron a agua destilada después de estar bajo los tratamientos de PEG o salinidad fue variable, dependiendo de la población, lo que sugiere que las semillas de P. lanceolata son moderadamente tolerantes a los estreses osmótico y salino en la etapa germinativa.
- Published
- 2018
26. Seed germination of Phillyrea angustifolia L., a species of difficult propagation
- Author
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Mira, Sara, primary, Arnal, Alberto, additional, and Pérez-García, Félix, additional
- Published
- 2017
- Full Text
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27. Marine green macroalgae: a source of natural compounds with mineralogenic and antioxidant activities
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Surget, Gwladys, Roberto, Vania P., Le Lann, Klervi, Mira, Sara, Guerard, Fabienne, Laize, Vincent, Poupart, Nathalie, Leonor Cancela, M., Stiger-pouvreau, Valerie, Surget, Gwladys, Roberto, Vania P., Le Lann, Klervi, Mira, Sara, Guerard, Fabienne, Laize, Vincent, Poupart, Nathalie, Leonor Cancela, M., and Stiger-pouvreau, Valerie
- Abstract
Marine macroalgae represent a valuable natural resource for bioactive phytochemicals with promising applications in therapeutics, although they remain largely under-exploited. In this work, the potential of two marine green macroalgae (Cladophora rupestris and Codium fragile) as a source of bioactive phenolic compounds was explored, and antioxidant, mineralogenic, and osteogenic activities were evaluated. For each species, a crude hydroalcoholic extract (CE) was prepared by solid/liquid extraction and fractionated by liquid/liquid purification into an ethyl acetate fraction (EAF) enriched in phenolic compounds and an aqueous fraction (AF). Antioxidant activity, assessed through radical scavenging activity and reducing power assay, was increased in EAF fraction of both species and closely related to the phenolic content in each fraction. Mineralogenic activity, assessed through extracellular matrix mineralization of a fish bone-derived cell line, was induced by EAF fractions (up to 600 % for C. rupestris EAF). Quantitative analysis of operculum formation in zebrafish larvae stained with alizarin red S further confirmed the osteogenic potential of EAF fractions in vivo, with an increase of more than 1.5-fold for both C. fragile and C. rupestris fractions, similar to vitamin D (control). Our results demonstrated a positive correlation between phenolic fractions and biological activity, suggesting that phenolic compounds extracted from marine green macroalgae may represent promising molecules toward therapeutic applications in the field of bone biology.
- Published
- 2017
- Full Text
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28. Seed germination of Phillyrea angustifolia L., a species of difficult propagation
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Mira, Sara, Lafarga Arnal, Alberto, Pérez García, Félix, Mira, Sara, Lafarga Arnal, Alberto, and Pérez García, Félix
- Abstract
Aim of study: The purpose was to determine the type of dormancy and the optimal germination conditions of Phillyrea angustifolia (Oleaceae) seeds.Area of study: Germination requirements of P. angustifolia seeds collected from wild plants growing in the province of Ávila (Central Spain) were studied.Materials and methods: Seed water uptake was measured. Seeds with and without an endocarp were germinated at different temperatures, and several treatments were tested.Main results: The lignified endocarp interferes mechanically with the emergence of the radicle, and the treatments that achieved the highest germination percentages were the total removal of the endocarp with pliers (84%) or the immersion in liquid nitrogen for 1 min (97%). Scarification with concentrated sulphuric acid did not significantly increase germination compared to the control seeds, and treatments with dry heat or wet heat were detrimental to seed germination. The optimum temperature for germination was 15 ºC. A pre-sowing treatment of soaking in distilled water for 24 h slightly increased germination speed. Neither cold stratification at 5 ºC nor soaking in a gibberellic acid solution improved seed germination. Research highlights: Phillyrea angustifolia seeds have physiological dormancy – that is, the embryo does not have enough growth potential to overcome the mechanical restriction of the lignified endocarp. The seeds do not exhibit physical dormancy, given their water-permeable lignified endocarp. Our results suggest that the optimum germination protocol for P. angustifolia would be the total removal of the endocarp or immersion in liquid nitrogen for 1 min, followed by immersion in distilled water for 24 h and then seed incubation at 15 ºC in light or darkness.
- Published
- 2017
29. Esclerites : quando a oftalmologia é a porta para uma doença sistémica
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Mira, Sara Lourenço Côrte-Real and Almeida, Leonor
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Oftalmologia ,Esclerite ,Ciências Médicas [Domínio/Área Científica] - Abstract
Trabalho Final do Curso de Mestrado Integrado em Medicina, Faculdade de Medicina, Universidade de Lisboa, 2015 Submitted by Biblioteca da Faculdade de Medicina da Universidade de Lisboa (repositorio@medicina.ulisboa.pt) on 2017-01-23T13:57:26Z No. of bitstreams: 1 SaraLRMira.pdf: 1363420 bytes, checksum: 86cb36c087a23fc3a10be81224f4ed5c (MD5) Made available in DSpace on 2017-01-23T13:57:40Z (GMT). No. of bitstreams: 1 SaraLRMira.pdf: 1363420 bytes, checksum: 86cb36c087a23fc3a10be81224f4ed5c (MD5) Previous issue date: 2015
- Published
- 2015
30. Sperm competition: effects on fertilization success of dusky grouper
- Author
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Engrola, Sofía, Cabrita, Elsa, Mira, Sara, Rocha, Filipa, Teixeira, Helena, Pousão-Ferreira, P., Cancela, M. Leonor, and Dinis, M. T.
- Abstract
Trabajo presentad en el World Aquaculture 2011, celebrado en Natal (Brasil) del 6 al 10 de junio de 2011., Currently, several species of grouper are already being produced in Asia for commercial purposes. Several individuals are kept as broodstock in research facilities throughout the Mediterranean.
- Published
- 2011
31. Conductivity test in seeds of different passion flower species
- Author
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Mira, Sara, primary, Veiga-Barbosa, Luciana, additional, González-Benito, María Elena, additional, and Pérez-García, Félix, additional
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- 2015
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32. Seed germination characteristics of Phillyrea angustifolia L. and P. latifolia L. (Oleaceae), two Mediterranean shrub species having lignified endocarp
- Author
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Mira, Sara, primary, Veiga-Barbosa, Luciana, additional, and Pérez-García, Félix, additional
- Published
- 2015
- Full Text
- View/download PDF
33. Avanços recentes em nutrição de larvas de peixes
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Conceição, Luís Eugénio Castanheira da, Aragão, Cláudia, Richard, Nadège, Engrola, Sofia, Gavaia, Paulo, Mira, Sara, and Dias, Jorge
- Subjects
proteómica ,proteomics ,programação nutricional ,genomics ,nutrição de larvas de peixes ,nutritional programming ,estudos com marcadores ,genómica ,fish larvae nutrition ,tracer studies - Abstract
Os requisitos nutricionais de larvas de peixes são ainda mal compreendidos, o que leva a altas mortalidades e problemas de qualidade no seu cultivo. Este trabalho pretende fazer uma revisão de novas metodologias de investigação, tais como estudos com marcadores, genómica populacional, programação nutricional, génomica e proteómica funcionais, e fornecer ainda alguns exemplos das utilizações presentes e perspectivas futuras em estudos de nutrição de larvas de peixes. Major gaps in knowledge on fish larval nutritional requirements still remain, what leads to high mortalities and quality problems in marine larviculture. This paper reviews a range of new tools, such as tracer studies, population genomics, nutritional programming, functional genomics and proteomics, as well as some examples of their present use, and potential future applications in the study of fish larvae nutrition.
- Published
- 2009
34. Recent advances in nutrition of fish larval | Avanços recentes em nutrição de larvas de peixes
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Conceição, L. E. C., Aragão, C., Richard, Nadège, Engrola, S., Gavaia, Paulo J., Mira, Sara, and Dias, J.
- Subjects
estudo com marcadores ,proteómica ,programação nutricional ,nutrição de larvas de peixes ,genómica - Abstract
Submitted by Paulo Gavaia (pgavaia@ualg.pt) on 2014-05-30T10:05:46Z No. of bitstreams: 1 Avanços recentes em nutrição de larvas de peixes.pdf: 75116 bytes, checksum: 728a03d131b35c9646da86008536f71d (MD5) Approved for entry into archive by Pedro Sousa (a19661@ualg.pt) on 2014-06-25T09:13:19Z (GMT) No. of bitstreams: 2 Avanços recentes em nutrição de larvas de peixes.pdf: 75116 bytes, checksum: 728a03d131b35c9646da86008536f71d (MD5) 8753041680192686.zip: 69092 bytes, checksum: 482c5f4899029bb21c5d8512b89342fe (MD5) Made available in DSpace on 2014-06-25T09:13:19Z (GMT). No. of bitstreams: 2 Avanços recentes em nutrição de larvas de peixes.pdf: 75116 bytes, checksum: 728a03d131b35c9646da86008536f71d (MD5) 8753041680192686.zip: 69092 bytes, checksum: 482c5f4899029bb21c5d8512b89342fe (MD5) Previous issue date: 2009
- Published
- 2009
35. Population genetics of an endangered species: the Bonelli's eagle (Hieraaetus fasciatus)
- Author
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Mira, Sara, Cancela, Maria Leonor, Dias, Pedro Beja, and Dias, Paula
- Subjects
Microsatélites ,Diversidade genética ,Águia de Bonelli ,Efeito gargalo ,Isolamento comportamental ,Conservação genética ,Ciências Naturais::Ciências da Terra e do Ambiente [Domínio/Área Científica] - Abstract
Tese de Doutoramento, Biologia especialidade Biologia Populacional, Faculdade de Ciências e Tecnologia, Universidade do Algarve, 2006 A águia de Bonelli é uma espécie ameaçada na Europa. No início dos anos 1980 elevadas taxas de mortalidade, devido a perseguição pela caça e electrocussão em linhas de alta tensão, provocaram um declínio acentuado nas populações. Actualmente existem cerca de 800 casais nidificantes, na Península Ibérica. As consequências genéticas de um decréscimo populacional são normalmente a perda de diversidade genética e o aumento dos níveis de consanguinidade, elevando assim o risco de extinção das populações. Com o objectivo de estudar a estrutura genética da ágia de Bonelli da Península Ibérica, foram desenvolvidos microsatélites para caracterizar quatro populações - Sudoeste de Portugal, Nordeste de Portugal, Cadiz e Estremadura. Os níveis de diversidade genética observados são moderados, excepto na população do Sudoeste de Portugal, onde foram observados baixos níveis de diversidade. Contrariamente ao esperado, nenhuma população revelou efeitos gargalo ou níveis de consanguinidade, o que sugere que a reprodução se faz de forma aleatória no interior de cada população. Contudo, as quatro populações estudadas apresentam uma forte estrutura espacial e genética significativa. Esta estrutura deve-se provavelmente ao comportamento filopátrico da espécie. Mais ainda a população do Sudoeste de Portugal apresenta indícios de um isolamento comportamental devido a apresentar uma estratégia de nidificação em árvores. Este trabalho termina sublinhando alguns aspectos importantes pra a preservação das populações estudadas de forma a manter a diversidade genética e comportamental existente.
- Published
- 2006
36. Diversité génétique et dynamique du recrutement chez l'huître plate Ostrea edulis L
- Author
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Lapegue, Sylvie, Boudry, Pierre, Mira, Sara, Taris, Nicolas, and Bonhomme, Francois
- Subjects
Diversité génétique ,Ostrea edulis ,Variabilité génétique ,Génétique population ,Huître plate ,Reproducteur - Abstract
In order to understand better some fundamental aspects of the bivalve biology, one has to pay attention to the variance of their breeding success, the time structure of the populations and their effective size. An abundant literature shows the existence of a heterozygote deficit as well as positive relationships between heterozygoty and fitness components. This last point is the subject of explanatory hypotheses which have never been clearly decided between until now. One of these hypotheses is the possible existence of a certain degree of inbreeding among those populations. This is only possible in the case of separated colonies coming from a small amount of spawners, thus creating blood relations between individuals recruited from the same area, and consequently a restricted local effective stock and the inbreeding of their offspring. In order to go deeper into this hypothesis, the interactions between genetics and demography at a population level have been studied on flat oyster larvae, spats and adults, through the analysis of five microsatellite markers. ..., Chez les Bivalves, la variance du succès reproducteur, la structure temporelle des populations et leur taille efficace sont autant d'éléments dont la connaissance est indispensable pour comprendre certains aspects fondamentaux de leur biologie. En particulier, une abondante littérature montre l'existence de déficits en hétérozygotes et de relations positives entre hétérozygotie et composantes de la valeur sélective. Ce dernier point fait l'objet d'hypothèses explicatives qui n'ont pas été clairement départagées jusqu'à présent. Une de ces hypothèses est l'existence possible d'un certain niveau de consanguinité dans les populations. Ceci n'est possible que s'il existe des cohortes séparées issues d'un nombre restreint de géniteurs, créant ainsi de l'apparentement entre individus recrutés au même endroit, et donc un effectif efficace local réduit et de la consanguinité dans leur descendance. Afin d'approfondir cette hypothèse, les interactions entre génétique et démographie à l'échelle de la population ont été étudiées chez des larves, naissains et adultes d'huître plate, grâce à l'analyse de 5 marqueurs microsatellites. Nous avons réalisé trois études complémentaires qui nous ont permis d'étudier : - la variance des fréquences alléliques au cours d'une saison de recrutement dans une population naturelle Les tailles efficaces des populations ont été estimées en comparant la variabilité observée dans les 3 cohortes recrutées à celle observée chez les adultes. - les contributions paternelles en réalisant des échantillonnages de larves non encore émises de femelles incubantes. La variabilité génétique de chaque "portée" (famille maternelle) a été analysée et comparée avec la variabilité observée au sein de la population adulte. - la variance du succès reproducteur individuel au sein d'une population expérimentale en écloserie.
- Published
- 2002
37. Large-scale population genetic structure in Bonelli's Eagle Aquila fasciata
- Author
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Mira, Sara, Arnaud-haond, Sophie, Palma, Luis, Cancela, Maria Leonor, Beja, Pedro, Mira, Sara, Arnaud-haond, Sophie, Palma, Luis, Cancela, Maria Leonor, and Beja, Pedro
- Abstract
In bird species that have a high movement capacity, dispersal may connect subpopulations over vast geographical regions, with important consequences for the design of conservation management strategies. Here we used a molecular approach to infer the patterns and rates of dispersal among eight Mediterranean subpopulations of the endangered Bonelli's Eagle, based on 245 individuals screened at 17 microsatellite loci. There was moderate genetic differentiation between subpopulations sampled in the western (Iberia and Morocco) and eastern (Cyprus) Mediterranean, whereas differentiation among subpopulations in the former region was weak to moderate and followed a pattern of isolation by distance. Within the western Mediterranean, the small, peripheral and ecologically unique population of southwest Portugal had the lowest genetic diversity and the highest differentiation. The remaining subpopulations formed two loose clusters, one including Morocco and southwest and eastern Spain, and the other northeast Portugal and western and central Spain. Few recent migrants were detected, and they originated primarily from adjacent subpopulations. Our findings suggest that western Mediterranean Bonelli's Eagles may have a large-scale metapopulation structure, with subpopulations connected to some extent by distance-dependent dispersal, probably influenced by natal philopatry and the geographical configuration of subpopulations. The combination of marked ecological and genetic divergence suggests that the peripheral subpopulation of southwest Portugal may be regarded as a distinct management unit.
- Published
- 2013
- Full Text
- View/download PDF
38. Germination and electrical conductivity tests on artificially aged seed lots of 2 wall-rocket species
- Author
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LAZAR, Simona-Laura, primary, MIRA, Sara, additional, PAMFIL, Doru, additional, and MARTÍNEZ-LABORDE, Juan Bautista, additional
- Published
- 2014
- Full Text
- View/download PDF
39. Population genetics of the European flat oyster (Ostrea edulis) : from larvae to populations
- Author
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Boudry, Pierre, Launey, Sophie, Diaz-almela, E., Naciri-graven, Yamama, Ledu, Christophe, Mira, Sara, Taris, Nicolas, Bonhomme, Francois, Lapegue, Sylvie, Boudry, Pierre, Launey, Sophie, Diaz-almela, E., Naciri-graven, Yamama, Ledu, Christophe, Mira, Sara, Taris, Nicolas, Bonhomme, Francois, and Lapegue, Sylvie
- Abstract
Nowadays, PCR-based DNA markers allow the study of diversity and differentiation of marine organisms at different stages of their life cycle. Our study of European flat oyster (Ostrea edulis) populations has aimed to describe their spatial and temporal dynamics. First, we analyzed adults sampled over the natural range of the species. An Atlantic/Mediterranean differentiation pattern was observed. Results were congruent between allozymes, microsatellites and mitochondrial DNA and support an isolation by distance model. High variability was observed in the breadth of diversity between populations using a mitochondrial marker, this highlights the small effective population sizes in some locations. A 10-fold quantitative difference was observed in Fst between the genomes, which may be due to an unbalanced sex ratio and/or differential reproductive success. Secondly, we investigated the dynamics of reproduction at a finer scale. Several experiments were performed to study (1) the variance of allele frequencies during a natural settlement period, (2) the paternal contribution to fertilization by analyzing larvae sampled at the brooding stage within individual females, (3) the individual reproductive success within an experimental population. The significance of our results will be discussed in terms of management of the genetic diversity and the potential impact of aquaculture and hatchery propagation.
- Published
- 2002
40. Avanços recentes em nutrição de larvas de peixes
- Author
-
Conceição, Luís Eugénio Castanheira da, primary, Aragão, Cláudia, additional, Richard, Nadège, additional, Engrola, Sofia, additional, Gavaia, Paulo, additional, Mira, Sara, additional, and Dias, Jorge, additional
- Published
- 2009
- Full Text
- View/download PDF
41. Lipid Thermal Fingerprints of Long-term Stored Seeds of Brassicaceae.
- Author
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Mira, Sara, Nadarajan, Jayanthi, Liu, Udayangani, González-Benito, Maria Elena, and Pritchard, Hugh W.
- Subjects
PLANT germplasm ,SEEDS ,DIFFERENTIAL scanning calorimetry ,BRASSICACEAE ,SEED viability - Abstract
Thermal fingerprints for seeds of 20 crop wild relatives of Brassicaceae stored for 8 to 44 years at the Plant Germplasm Bank—Universidad Politécnica de Madrid and the Royal Botanic Gardens, Kew's Millennium Seed Bank—were generated using differential scanning calorimetry (DSC) and analyzed in relation to storage stability. Relatively poor storing oily seeds at −20 °C tended to have lipids with crystallization and melting transitions spread over a wide temperature range (c. 40 °C) that spanned the storage temperature, plus a melting end temperature of around 15 °C. We postulated that in dry storage, the variable longevity in Brassicaceae seeds could be associated with the presence of a metastable lipid phase at the temperature at which they are being stored. Consistent with that, when high-quality seed samples of various species were assessed after banking at −5 to −10 °C for c. 40 years, melting end temperatures were observed to be much lower (c. 0 to −30 °C) and multiple lipid phases did not occur at the storage temperature. We conclude that multiple features of the seed lipid thermal fingerprint could be used as biophysical markers to predict potential poor performance of oily seeds during long-term, decadal storage. [ABSTRACT FROM AUTHOR]
- Published
- 2019
- Full Text
- View/download PDF
42. Population genetics of the European flat oyster (Ostrea edulis) : from larvae to populations
- Author
-
Pierre Boudry, Launey, Sophie, Diaz-Almela, E., Naciri-Graven, Yamama, Ledu, Christophe, Mira, Sara, Taris, Nicolas, Bonhomme, Francois, and Sylvie Lapegue
- Subjects
European flat oyster ,Larvae ,Genetic ,Ostrea edulis ,Population genetics ,Reproduction - Abstract
Nowadays, PCR-based DNA markers allow the study of diversity and differentiation of marine organisms at different stages of their life cycle. Our study of European flat oyster (Ostrea edulis) populations has aimed to describe their spatial and temporal dynamics. First, we analyzed adults sampled over the natural range of the species. An Atlantic/Mediterranean differentiation pattern was observed. Results were congruent between allozymes, microsatellites and mitochondrial DNA and support an isolation by distance model. High variability was observed in the breadth of diversity between populations using a mitochondrial marker, this highlights the small effective population sizes in some locations. A 10-fold quantitative difference was observed in Fst between the genomes, which may be due to an unbalanced sex ratio and/or differential reproductive success. Secondly, we investigated the dynamics of reproduction at a finer scale. Several experiments were performed to study (1) the variance of allele frequencies during a natural settlement period, (2) the paternal contribution to fertilization by analyzing larvae sampled at the brooding stage within individual females, (3) the individual reproductive success within an experimental population. The significance of our results will be discussed in terms of management of the genetic diversity and the potential impact of aquaculture and hatchery propagation.
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