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Your search keyword '"Mu, Xinhao"' showing total 11 results
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11 results on '"Mu, Xinhao"'

7. Rapid and visual detection of novel astroviruses causing fatal gout in goslings using one-step reverse transcription loop-mediated isothermal amplification

Author

Chaoge Sui, Lunguang Yao, Qingmei Xie, Qinxi Chen, Zhengli Yu, Yunchao Kan, Zhang Zhibin, Jun Ji, Mu Xinhao, Wen Hu, and Xin Xu

Subjects
Gout, DNA polymerase, Loop-mediated isothermal amplification, N-GoAstV, Sensitivity and Specificity, Article, law.invention, 03 medical and health sciences, chemistry.chemical_compound, law, Astroviridae Infections, Geese, Animals, Reverse Transcription Loop-mediated Isothermal Amplification, Polymerase chain reaction, 030304 developmental biology, lcsh:SF1-1100, Detection limit, Phenol red, 0303 health sciences, biology, 0402 animal and dairy science, one-step RT-LAMP, 04 agricultural and veterinary sciences, General Medicine, visible detection, 040201 dairy & animal science, Molecular biology, Reverse transcriptase, rapidity, chemistry, Molecular Diagnostic Techniques, biology.protein, Astroviridae, Animal Science and Zoology, lcsh:Animal culture, Primer (molecular biology), Nucleic Acid Amplification Techniques
Abstract

To visually and rapidly detect a novel goose astrovirus (N-GoAstV) causing fatal gout in goslings, an isothermal detection method based on one-step reverse transcription loop-mediated isothermal amplification (one-step RT-LAMP) was established. The one-step RT-LAMP assay for N-GoAstV detection, using Bst 3.0 DNA polymerase with strong reverse transcription activity and primer sets targeting the opening reading frame 1b (ORF1b) of N-GoAstV, could be completed in 30 min using a water bath at 61°C; the detection results could be visually observed by adding a pH-sensitive dye containing phenol red and cresol red. The detection limit of the one-step RT-LAMP assay was 57.8 copies/μL, which was similar to that of reverse transcription–quantitative polymerase chain reaction. The assay specifically detected N-GoAstV without any cross-reaction with other reference viruses, and this was further confirmed using enzyme digestion. These results indicated that the newly established RT-LAMP assay could accomplish reverse transcription, amplification, and visual result determination in one step, and the results obtained via this rapid and cost-effective method could be used to support disease control on farms in terms of N-GoAstV infection.

Published
2020

8. Simple and Visible Detection of Novel Astroviruses Causing Fatal Gout in Goslings Using One-Step Reverse Transcription Polymerase Spiral Reaction Method

Author

Xin Xu, Yingzuo Bi, Xiaoge Fu, Xiang Tian, Qinxi Chen, Zhengli Yu, Mu Xinhao, Qingmei Xie, Lunguang Yao, and Jun Ji

Subjects
Detection limit, 0303 health sciences, lcsh:Veterinary medicine, General Veterinary, biology, 030306 microbiology, Specific detection, Brief Research Report, N-GoAstV, simplicity, visible detection, Virology, Disease control, Reverse transcriptase, 03 medical and health sciences, rapidity, biology.protein, RT-PSR, lcsh:SF600-1100, Veterinary Science, Polymerase, 030304 developmental biology
Abstract

In this study, a one-step isothermal method combining polymerase spiral reaction (PSR) with reverse transcription (RT-PSR) was established for rapid and specific detection of novel astroviruses causing fatal gout in goslings (N-GoAstV). The one-step RT-PSR was accomplished at the optimal temperature of 62°C and time of 40 min and used primers simply designed as conventional PCR primers, and the results of detection were visible to the naked eye. The detection limit of PSR was above 34.7 copies/μL at a 95% probability level according to probit regression analysis. The assay specifically detected N-GoAstV, and no other reference viruses were detected. These results suggest that the newly established RT-PSR assay could, in one step, accomplish reverse-transcription, amplification, and result determination providing a visible, convenient, rapid, and cost-effective test that can be carried out onsite, in order to ensure timely quarantine of N-GoAstV-infected birds, leading to effective disease control.

Published
2020

11. Conservation and distribution of the DRACH motif for potential m 6 A sites in avian leukosis virus subgroup J.

Author

Ji J, Mu X, Xu S, Xu X, Zhang Z, Yao L, Xie Q, and Bi Y

Abstract

N 6 -methyladenosine (m 6 A) methylation is an internal post-transcriptional modification that has been linked to viral multiplication and pathogenicity. To elucidate the conservation patterns of potential 5'-DRACH-3' motifs in avian leukosis virus subgroup J (ALV-J), 149 ALV-J strains (139 isolates from China; ALV-J prototype HPRS-103 from the UK; and 9 strains from the USA, Russia, India, and Pakistan) available in GenBank before December 2023 were retrieved. According to the prediction results of the SRAMP web-server, these ALV-J genomes contained potential DRACH motifs, with the total number ranging from 43 to 64, which were not determined based on the isolation region and time. Conservative analysis suggested that 37 motifs exhibited a conservation of >80%, including 17 motifs with a grading above "high confidence." Although these motifs were distributed in the U5 region of LTRs and major coding regions, they were enriched in the coding regions of p27 , p68 , p32 , and gp85 . The most common m 6 A-motif sequence of the DRACH motif in the ALV-J genome was GGACU. The RNA secondary structure of each conserved motif predicted by SRAMP and RNAstructure web-server was mainly of two types-A-U pair (21/37) and hairpin loop (16/37)-based on the core adenosine. Considering the systematic comparative analysis performed in this study, future thorough biochemical research is warranted to determine the role of m 6 A modification during the replication and infection of ALV-J. These conservation and distribution analysis of the DRACH motif for potential m 6 A sites in ALV-J would provide a foundation for the future intervention of ALV-J infection and m 6 A modification., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2024 Ji, Mu, Xu, Xu, Zhang, Yao, Xie and Bi.)

Published
2024
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