67 results on '"Nánási PP"'
Search Results
2. Role of action potential configuration and the contribution of Ca2+and K+currents to isoprenaline-induced changes in canine ventricular cells
- Author
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Szentandrássy, N, primary, Farkas, V, additional, Bárándi, L, additional, Hegyi, B, additional, Ruzsnavszky, F, additional, Horváth, B, additional, Bányász, T, additional, Magyar, J, additional, Márton, I, additional, and Nánási, PP, additional
- Published
- 2012
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3. Analysis of the contribution of Ito to repolarization in canine ventricular myocardium
- Author
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Virág, L, primary, Jost, N, additional, Papp, R, additional, Koncz, I, additional, Kristóf, A, additional, Kohajda, Z, additional, Harmati, G, additional, Carbonell-Pascual, B, additional, Ferrero Jr, JM, additional, Papp, JG, additional, Nánási, PP, additional, and Varró, A, additional
- Published
- 2011
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4. Effects of rosiglitazone on the configuration of action potentials and ion currents in canine ventricular cells
- Author
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Szentandrássy, N, primary, Harmati, G, additional, Bárándi, L, additional, Simkó, J, additional, Horváth, B, additional, Magyar, J, additional, Bányász, T, additional, Lőrincz, I, additional, Szebeni, A, additional, Kecskeméti, V, additional, and Nánási, PP, additional
- Published
- 2011
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5. Role of action potential configuration and the contribution of C²⁺a and K⁺ currents to isoprenaline-induced changes in canine ventricular cells.
- Author
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Szentandrássy N, Farkas V, Bárándi L, Hegyi B, Ruzsnavszky F, Horváth B, Bányász T, Magyar J, Márton I, Nánási PP, Szentandrássy, N, Farkas, V, Bárándi, L, Hegyi, B, Ruzsnavszky, F, Horváth, B, Bányász, T, Magyar, J, Márton, I, and Nánási, P P
- Abstract
Background and Purpose: Although isoprenaline (ISO) is known to activate several ion currents in mammalian myocardium, little is known about the role of action potential morphology in the ISO-induced changes in ion currents. Therefore, the effects of ISO on action potential configuration, L-type Ca²⁺ current (I(Ca)), slow delayed rectifier K⁺ current (I(Ks)) and fast delayed rectifier K⁺ current (I(Kr)) were studied and compared in a frequency-dependent manner using canine isolated ventricular myocytes from various transmural locations.Experimental Approach: Action potentials were recorded with conventional sharp microelectrodes; ion currents were measured using conventional and action potential voltage clamp techniques.Key Results: In myocytes displaying a spike-and-dome action potential configuration (epicardial and midmyocardial cells), ISO caused reversible shortening of action potentials accompanied by elevation of the plateau. ISO-induced action potential shortening was absent in endocardial cells and in myocytes pretreated with 4-aminopyridine. Application of the I(Kr) blocker E-4031 failed to modify the ISO effect, while action potentials were lengthened by ISO in the presence of the I(Ks) blocker HMR-1556. Both action potential shortening and elevation of the plateau were prevented by pretreatment with the I(Ca) blocker nisoldipine. Action potential voltage clamp experiments revealed a prominent slowly inactivating I(Ca) followed by a rise in I(Ks) , both currents increased with increasing the cycle length.Conclusions and Implications: The effect of ISO in canine ventricular cells depends critically on action potential configuration, and the ISO-induced activation of I(Ks) - but not I(Kr) - may be responsible for the observed shortening of action potentials. [ABSTRACT FROM AUTHOR]- Published
- 2012
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6. Role of action potential configuration and the contribution of Ca2+ and K+ currents to isoprenaline-induced changes in canine ventricular cells.
- Author
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Szentandrássy, N, Farkas, V, Bárándi, L, Hegyi, B, Ruzsnavszky, F, Horváth, B, Bányász, T, Magyar, J, Márton, I, and Nánási, PP
- Subjects
ACTION potentials ,CALCIUM ions ,ELECTROPHYSIOLOGY ,POTASSIUM in the body ,MYOCARDIUM physiology ,ISOPROTERENOL ,HEART ventricles ,HEART cells ,LABORATORY dogs - Abstract
BACKGROUND AND PURPOSE Although isoprenaline (ISO) is known to activate several ion currents in mammalian myocardium, little is known about the role of action potential morphology in the ISO-induced changes in ion currents. Therefore, the effects of ISO on action potential configuration, L-type Ca
2+ current ( ICa ), slow delayed rectifier K+ current ( IKs ) and fast delayed rectifier K+ current ( IKr ) were studied and compared in a frequency-dependent manner using canine isolated ventricular myocytes from various transmural locations. EXPERIMENTAL APPROACH Action potentials were recorded with conventional sharp microelectrodes; ion currents were measured using conventional and action potential voltage clamp techniques. KEY RESULTS In myocytes displaying a spike-and-dome action potential configuration (epicardial and midmyocardial cells), ISO caused reversible shortening of action potentials accompanied by elevation of the plateau. ISO-induced action potential shortening was absent in endocardial cells and in myocytes pretreated with 4-aminopyridine. Application of the IKr blocker E-4031 failed to modify the ISO effect, while action potentials were lengthened by ISO in the presence of the IKs blocker HMR-1556. Both action potential shortening and elevation of the plateau were prevented by pretreatment with the ICa blocker nisoldipine. Action potential voltage clamp experiments revealed a prominent slowly inactivating ICa followed by a rise in IKs , both currents increased with increasing the cycle length. CONCLUSIONS AND IMPLICATIONS The effect of ISO in canine ventricular cells depends critically on action potential configuration, and the ISO-induced activation of IKs - but not IKr - may be responsible for the observed shortening of action potentials. [ABSTRACT FROM AUTHOR]- Published
- 2012
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7. Relationship between ion currents and membrane capacitance in canine ventricular myocytes.
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Horváth B, Kovács Z, Dienes C, Barta Z, Óvári J, Szentandrássy N, Magyar J, Bányász T, and Nánási PP
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- Animals, Dogs, Membrane Potentials physiology, Ion Channels metabolism, Cell Membrane metabolism, Myocytes, Cardiac metabolism, Myocytes, Cardiac physiology, Electric Capacitance, Heart Ventricles cytology, Heart Ventricles metabolism, Action Potentials physiology, Patch-Clamp Techniques
- Abstract
Current density, the membrane current value divided by membrane capacitance (C
m ), is widely used in cellular electrophysiology. Comparing current densities obtained in different cell populations assume that Cm and ion current magnitudes are linearly related, however data is scarce about this in cardiomyocytes. Therefore, we statistically analyzed the distributions, and the relationship between parameters of canine cardiac ion currents and Cm , and tested if dividing original parameters with Cm had any effect. Under conventional voltage clamp conditions, correlations were high for IK1 , moderate for IKr and ICa,L , while negligible for IKs . Correlation between Ito1 peak amplitude and Cm was negligible when analyzing all cells together, however, the analysis showed high correlations when cells of subepicardial, subendocardial or midmyocardial origin were analyzed separately. In action potential voltage clamp experiments IK1, IKr and ICa,L parameters showed high correlations with Cm . For INCX , INa,late and IKs there were low-to-moderate correlations between Cm and these current parameters. Dividing the original current parameters with Cm reduced both the coefficient of variation, and the deviation from normal distribution. The level of correlation between ion currents and Cm varies depending on the ion current studied. This must be considered when evaluating ion current densities in cardiac cells., (© 2024. The Author(s).)- Published
- 2024
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8. Astrocyte- and NMDA receptor-dependent slow inward currents differently contribute to synaptic plasticity in an age-dependent manner in mouse and human neocortex.
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Csemer A, Kovács A, Maamrah B, Pocsai K, Korpás K, Klekner Á, Szücs P, Nánási PP, and Pál B
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- Mice, Humans, Animals, Receptors, N-Methyl-D-Aspartate metabolism, Neurons metabolism, Neuronal Plasticity, Synapses metabolism, Astrocytes metabolism, Neocortex metabolism
- Abstract
Slow inward currents (SICs) are known as excitatory events of neurons elicited by astrocytic glutamate via activation of extrasynaptic NMDA receptors. By using slice electrophysiology, we tried to provide evidence that SICs can elicit synaptic plasticity. Age dependence of SICs and their impact on synaptic plasticity was also investigated in both on murine and human cortical slices. It was found that SICs can induce a moderate synaptic plasticity, with features similar to spike timing-dependent plasticity. Overall SIC activity showed a clear decline with aging in humans and completely disappeared above a cutoff age. In conclusion, while SICs contribute to a form of astrocyte-dependent synaptic plasticity both in mice and humans, this plasticity is differentially affected by aging. Thus, SICs are likely to play an important role in age-dependent physiological and pathological alterations of synaptic plasticity., (© 2023 The Authors. Aging Cell published by Anatomical Society and John Wiley & Sons Ltd.)
- Published
- 2023
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9. Selective Inhibition of Cardiac Late Na + Current Is Based on Fast Offset Kinetics of the Inhibitor.
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Naveed M, Mohammed ASA, Topal L, Kovács ZM, Dienes C, Ovári J, Szentandrássy N, Magyar J, Bányász T, Prorok J, Jost N, Virág L, Baczkó I, Varró A, Nánási PP, and Horváth B
- Abstract
The present study was designed to test the hypothesis that the selectivity of blocking the late Na
+ current (INaL ) over the peak Na+ current (INaP ) is related to the fast offset kinetics of the Na+ channel inhibitor. Therefore, the effects of 1 µM GS967 (INaL inhibitor), 20 µM mexiletine (I/B antiarrhythmic) and 10 µM quinidine (I/A antiarrhythmic) on INaL and INaP were compared in canine ventricular myocardium. INaP was estimated as the maximum velocity of action potential upstroke (V+ max ). Equal amounts of INaL were dissected by the applied drug concentrations under APVC conditions. The inhibition of INaL by mexiletine and quinidine was comparable under a conventional voltage clamp, while both were smaller than the inhibitory effect of GS967. Under steady-state conditions, the V+ max block at the physiological cycle length of 700 ms was 2.3% for GS967, 11.4% for mexiletine and 26.2% for quinidine. The respective offset time constants were 110 ± 6 ms, 456 ± 284 ms and 7.2 ± 0.9 s. These results reveal an inverse relationship between the offset time constant and the selectivity of INaL over INaP inhibition without any influence of the onset rate constant. It is concluded that the selective inhibition of INaL over INaP is related to the fast offset kinetics of the Na+ channel inhibitor.- Published
- 2023
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10. Conductance Changes of Na + Channels during the Late Na + Current Flowing under Action Potential Voltage Clamp Conditions in Canine, Rabbit, and Guinea Pig Ventricular Myocytes.
- Author
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Horváth B, Kovács ZM, Dienes C, Óvári J, Szentandrássy N, Magyar J, Bányász T, Varró A, and Nánási PP
- Abstract
Late sodium current (I
Na,late ) is an important inward current contributing to the plateau phase of the action potential (AP) in the mammalian heart. Although INa,late is considered as a possible target for antiarrhythmic agents, several aspects of this current remained hidden. In this work, the profile of INa,late , together with the respective conductance changes (GNa,late ), were studied and compared in rabbit, canine, and guinea pig ventricular myocytes using the action potential voltage clamp (APVC) technique. In canine and rabbit myocytes, the density of INa,late was relatively stable during the plateau and decreased only along terminal repolarization of the AP, while GNa,late decreased monotonically. In contrast, INa,late increased monotonically, while GNa,late remained largely unchanged during the AP in guinea pig. The estimated slow inactivation of Na+ channels was much slower in guinea pig than in canine or rabbit myocytes. The characteristics of canine INa,late and GNa,late were not altered by using command APs recorded from rabbit or guinea pig myocytes, indicating that the different shapes of the current profiles are related to genuine interspecies differences in the gating of INa,late . Both INa,late and GNa,late decreased in canine myocytes when the intracellular Ca2+ concentration was reduced either by the extracellular application of 1 µM nisoldipine or by the intracellular application of BAPTA. Finally, a comparison of the INa,late and GNa,late profiles induced by the toxin of Anemonia sulcata (ATX-II) in canine and guinea pig myocytes revealed profound differences between the two species: in dog, the ATX-II induced INa,late and GNa,late showed kinetics similar to those observed with the native current, while in guinea pig, the ATX-II induced GNa,late increased during the AP. Our results show that there are notable interspecies differences in the gating kinetics of INa,late that cannot be explained by differences in AP morphology. These differences must be considered when interpreting the INa,late results obtained in guinea pig.- Published
- 2023
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11. Omecamtiv mecarbil augments cardiomyocyte contractile activity both at resting and systolic Ca 2+ levels.
- Author
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Ráduly AP, Tóth A, Sárkány F, Horváth B, Szentandrássy N, Nánási PP, Csanádi Z, Édes I, Papp Z, and Borbély A
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- Animals, Dogs, Stroke Volume, Simendan pharmacology, Myosins, Myocytes, Cardiac metabolism, Heart Failure
- Abstract
Aims: Heart failure with reduced ejection fraction (HFrEF) is a disease with high mortality and morbidity. Recent positive inotropic drug developments focused on cardiac myofilaments, that is, direct activators of the myosin molecule and Ca
2+ sensitizers for patients with advanced HFrEF. Omecamtiv mecarbil (OM) is the first direct myosin activator with promising results in clinical studies. Here, we aimed to elucidate the cellular mechanisms of the positive inotropic effect of OM in a comparative in vitro investigation where Ca2+ -sensitizing positive inotropic agents with distinct mechanisms of action [EMD 53998 (EMD), which also docks on the myosin molecule, and levosimendan (Levo), which binds to troponin C] were included., Methods: Enzymatically isolated canine cardiomyocytes with intact cell membranes were loaded with Fura-2AM, a Ca2+ -sensitive, ratiometric, fluorescent dye. Changes in sarcomere length (SL) and intracellular Ca2+ concentration were recorded in parallel at room temperature, whereas cardiomyocyte contractions were evoked by field stimulation at 0.1 Hz in the presence of different OM, EMD, or Levo concentrations., Results: SL was reduced by about 23% or 9% in the presence of 1 μM OM or 1 μM EMD in the absence of electrical stimulation, whereas 1 μM Levo had no effect on resting SL. Fractional sarcomere shortening was increased by 1 μM EMD or 1 μM Levo to about 152%, but only to about 128% in the presence of 0.03 μM OM. At higher OM concentrations, no significant increase in fractional sarcomere shortening could be recorded. Contraction durations largely increased, whereas the kinetics of contractions and relaxations decreased with increasing OM concentrations. One-micromole EMD or 1 μM Levo had no effects on contraction durations. One-micromole Levo, but not 1 μM EMD, accelerated the kinetics of cardiomyocyte contractions and relaxations. Ca2+ transient amplitudes were unaffected by all treatments., Conclusions: Our data revealed major distinctions between the cellular effects of myofilament targeted agents (OM, EMD, or Levo) depending on their target proteins and binding sites, although they were compatible with the involvement of Ca2+ -sensitizing mechanisms for all three drugs. Significant part of the cardiotonic effect of OM relates to the prolongation of systolic contraction in combination with its Ca2+ -sensitizing effect., (© 2023 The Authors. ESC Heart Failure published by John Wiley & Sons Ltd on behalf of European Society of Cardiology.)- Published
- 2023
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12. ABT-333 (Dasabuvir) Increases Action Potential Duration and Provokes Early Afterdepolarizations in Canine Left Ventricular Cells via Inhibition of I Kr .
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Kovács ZM, Óvári J, Dienes C, Magyar J, Bányász T, Nánási PP, Horváth B, Feher A, Varga Z, and Szentandrássy N
- Abstract
ABT-333 (dasabuvir) is an antiviral agent used in hepatitis C treatment. The molecule, similarly to some inhibitors of hERG channels, responsible for the delayed rectifier potassium current (I
Kr ), contains the methanesulfonamide group. Reduced IKr current leads to long QT syndrome and early afterdepolarizations (EADs), therefore potentially causing life-threatening arrhythmias and sudden cardiac death. Our goal was to investigate the acute effects of ABT-333 in enzymatically isolated canine left ventricular myocardial cells. Action potentials (APs) and ion currents were recorded with a sharp microelectrode technique and whole-cell patch clamp, respectively. Application of 1 μM ABT-333 prolonged the AP in a reversible manner. The maximal rates of phases 0 and 1 were irreversibly decreased. Higher ABT-333 concentrations caused larger AP prolongation, elevation of the early plateau potential, and reduction of maximal rates of phases 0, 1, and 3. EADs occurred in some cells in 3-30 μM ABT-333 concentrations. The 10 μM ABT-333-sensitive current, recorded with AP voltage clamp, contained a late outward component corresponding to IKr and an early outward one corresponding to transient outward potassium current (Ito ). ABT-333 reduced hERG-channel-mediated ion current in a concentration-dependent, partially reversible manner with a half-inhibitory concentration of 3.2 μM. As the therapeutic plasma concentration of ABT-333 is 1 nM, the arrhythmic risk of ABT-333 is very low, even in the case of drug overdose.- Published
- 2023
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13. Antiarrhythmic and Inotropic Effects of Selective Na + /Ca 2+ Exchanger Inhibition: What Can We Learn from the Pharmacological Studies?
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Nagy N, Tóth N, and Nánási PP
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- Humans, Ion Channels metabolism, Arrhythmias, Cardiac drug therapy, Biological Transport physiology, Calcium metabolism, Sodium-Calcium Exchanger metabolism, Anti-Arrhythmia Agents pharmacology
- Abstract
Life-long stable heart function requires a critical balance of intracellular Ca
2+ . Several ion channels and pumps cooperate in a complex machinery that controls the influx, release, and efflux of Ca2+ . Probably one of the most interesting and most complex players of this crosstalk is the Na+ /Ca2+ exchanger, which represents the main Ca2+ efflux mechanism; however, under some circumstances, it can also bring Ca2+ into the cell. Therefore, the inhibition of the Na+ /Ca2+ exchanger has emerged as one of the most promising possible pharmacological targets to increase Ca2+ levels, to decrease arrhythmogenic depolarizations, and to reduce excessive Ca2+ influx. In line with this, as a response to increasing demand, several more or less selective Na+ /Ca2+ exchanger inhibitor compounds have been developed. In the past 20 years, several results have been published regarding the effect of Na+ /Ca2+ exchanger inhibition under various circumstances, e.g., species, inhibitor compounds, and experimental conditions; however, the results are often controversial. Does selective Na+ /Ca2+ exchanger inhibition have any future in clinical pharmacological practice? In this review, the experimental results of Na+ /Ca2+ exchanger inhibition are summarized focusing on the data obtained by novel highly selective inhibitors.- Published
- 2022
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14. Therapeutic Approaches of Ryanodine Receptor-Associated Heart Diseases.
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Szentandrássy N, Magyar ZÉ, Hevesi J, Bányász T, Nánási PP, and Almássy J
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- Arrhythmogenic Right Ventricular Dysplasia, Calcium metabolism, Calcium Signaling, Humans, Mutation, Sarcoplasmic Reticulum metabolism, Ryanodine Receptor Calcium Release Channel genetics, Ryanodine Receptor Calcium Release Channel metabolism, Tachycardia, Ventricular etiology, Tachycardia, Ventricular metabolism, Tachycardia, Ventricular therapy
- Abstract
Cardiac diseases are the leading causes of death, with a growing number of cases worldwide, posing a challenge for both healthcare and research. Therefore, the most relevant aim of cardiac research is to unravel the molecular pathomechanisms and identify new therapeutic targets. Cardiac ryanodine receptor (RyR2), the Ca
2+ release channel of the sarcoplasmic reticulum, is believed to be a good therapeutic target in a group of certain heart diseases, collectively called cardiac ryanopathies. Ryanopathies are associated with the impaired function of the RyR, leading to heart diseases such as congestive heart failure (CHF), catecholaminergic polymorphic ventricular tachycardia (CPVT), arrhythmogenic right ventricular dysplasia type 2 (ARVD2), and calcium release deficiency syndrome (CRDS). The aim of the current review is to provide a short insight into the pathological mechanisms of ryanopathies and discuss the pharmacological approaches targeting RyR2.- Published
- 2022
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15. Exploring the Coordination of Cardiac Ion Channels With Action Potential Clamp Technique.
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Horváth B, Szentandrássy N, Dienes C, Kovács ZM, Nánási PP, Chen-Izu Y, Izu LT, and Banyasz T
- Abstract
The patch clamp technique underwent continual advancement and developed numerous variants in cardiac electrophysiology since its introduction in the late 1970s. In the beginning, the capability of the technique was limited to recording one single current from one cell stimulated with a rectangular command pulse. Since that time, the technique has been extended to record multiple currents under various command pulses including action potential. The current review summarizes the development of the patch clamp technique in cardiac electrophysiology with special focus on the potential applications in integrative physiology., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2022 Horváth, Szentandrássy, Dienes, Kovács, Nánási, Chen-Izu, Izu and Banyasz.)
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- 2022
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16. Late Sodium Current of the Heart: Where Do We Stand and Where Are We Going?
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Horváth B, Szentandrássy N, Almássy J, Dienes C, Kovács ZM, Nánási PP, and Banyasz T
- Abstract
Late sodium current has long been linked to dysrhythmia and contractile malfunction in the heart. Despite the increasing body of accumulating information on the subject, our understanding of its role in normal or pathologic states is not complete. Even though the role of late sodium current in shaping action potential under physiologic circumstances is debated, it's unquestioned role in arrhythmogenesis keeps it in the focus of research. Transgenic mouse models and isoform-specific pharmacological tools have proved useful in understanding the mechanism of late sodium current in health and disease. This review will outline the mechanism and function of cardiac late sodium current with special focus on the recent advances of the area.
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- 2022
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17. Pharmacological Modulation and (Patho)Physiological Roles of TRPM4 Channel-Part 1: Modulation of TRPM4.
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Kovács ZM, Dienes C, Hézső T, Almássy J, Magyar J, Bányász T, Nánási PP, Horváth B, and Szentandrássy N
- Abstract
Transient receptor potential melastatin 4 is a unique member of the TRPM protein family and, similarly to TRPM5, is Ca
2+ -sensitive and permeable to monovalent but not divalent cations. It is widely expressed in many organs and is involved in several functions by regulating the membrane potential and Ca2+ homeostasis in both excitable and non-excitable cells. This part of the review discusses the pharmacological modulation of TRPM4 by listing, comparing, and describing both endogenous and exogenous activators and inhibitors of the ion channel. Moreover, other strategies used to study TRPM4 functions are listed and described. These strategies include siRNA-mediated silencing of TRPM4, dominant-negative TRPM4 variants, and anti-TRPM4 antibodies. TRPM4 is receiving more and more attention and is likely to be the topic of research in the future.- Published
- 2022
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18. Pharmacological Modulation and (Patho)Physiological Roles of TRPM4 Channel-Part 2: TRPM4 in Health and Disease.
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Dienes C, Kovács ZM, Hézső T, Almássy J, Magyar J, Bányász T, Nánási PP, Horváth B, and Szentandrássy N
- Abstract
Transient receptor potential melastatin 4 (TRPM4) is a unique member of the TRPM protein family and, similarly to TRPM5, is Ca
2+ sensitive and permeable for monovalent but not divalent cations. It is widely expressed in many organs and is involved in several functions; it regulates membrane potential and Ca2+ homeostasis in both excitable and non-excitable cells. This part of the review discusses the currently available knowledge about the physiological and pathophysiological roles of TRPM4 in various tissues. These include the physiological functions of TRPM4 in the cells of the Langerhans islets of the pancreas, in various immune functions, in the regulation of vascular tone, in respiratory and other neuronal activities, in chemosensation, and in renal and cardiac physiology. TRPM4 contributes to pathological conditions such as overactive bladder, endothelial dysfunction, various types of malignant diseases and central nervous system conditions including stroke and injuries as well as in cardiac conditions such as arrhythmias, hypertrophy, and ischemia-reperfusion injuries. TRPM4 claims more and more attention and is likely to be the topic of research in the future.- Published
- 2021
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19. Late sodium current and calcium homeostasis in arrhythmogenesis.
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Kistamás K, Hézső T, Horváth B, and Nánási PP
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- Humans, Animals, Action Potentials, Voltage-Gated Sodium Channels metabolism, Sodium-Calcium Exchanger metabolism, Calcium metabolism, Homeostasis, Arrhythmias, Cardiac metabolism, Arrhythmias, Cardiac physiopathology, Sodium metabolism
- Abstract
The cardiac late sodium current (I
Na,late ) is the small sustained component of the sodium current active during the plateau phase of the action potential. Several studies demonstrated that augmentation of the current can lead to cardiac arrhythmias; therefore, INa,late is considered as a promising antiarrhythmic target. Fundamentally, enlarged INa,late increases Na+ influx into the cell, which, in turn, is converted to elevated intracellular Ca2+ concentration through the Na+ /Ca2+ exchanger. The excessive Ca2+ load is known to be proarrhythmic. This review describes the behavior of the voltage-gated Na+ channels generating INa,late in health and disease and aims to discuss the physiology and pathophysiology of Na+ and Ca2+ homeostasis in context with the enhanced INa,late demonstrating also the currently accessible antiarrhythmic choices.- Published
- 2021
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20. Late Na + Current Is [Ca 2+ ] i -Dependent in Canine Ventricular Myocytes.
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Kiss D, Horváth B, Hézső T, Dienes C, Kovács Z, Topal L, Szentandrássy N, Almássy J, Prorok J, Virág L, Bányász T, Varró A, Nánási PP, and Magyar J
- Abstract
Enhancement of the late sodium current (I
NaL ) increases arrhythmia propensity in the heart, whereas suppression of the current is antiarrhythmic. In the present study, we investigated INaL in canine ventricular cardiomyocytes under action potential voltage-clamp conditions using the selective Na+ channel inhibitors GS967 and tetrodotoxin. Both 1 µM GS967 and 10 µM tetrodotoxin dissected largely similar inward currents. The amplitude and integral of the GS967-sensitive current was significantly smaller after the reduction of intracellular Ca2+ concentration ([Ca2+ ]i ) either by superfusion of the cells with 1 µM nisoldipine or by intracellular application of 10 mM BAPTA. Inhibiting calcium/calmodulin-dependent protein kinase II (CaMKII) by KN-93 or the autocamtide-2-related inhibitor peptide similarly reduced the amplitude and integral of INaL . Action potential duration was shortened in a reverse rate-dependent manner and the plateau potential was depressed by GS967. This GS967-induced depression of plateau was reduced by pretreatment of the cells with BAPTA-AM. We conclude that (1) INaL depends on the magnitude of [Ca2+ ]i in canine ventricular cells, (2) this [Ca2+ ]i -dependence of INaL is mediated by the Ca2+ -dependent activation of CaMKII, and (3) INaL is augmented by the baseline CaMKII activity.- Published
- 2021
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21. TRPM4 links calcium signaling to membrane potential in pancreatic acinar cells.
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Diszházi G, Magyar ZÉ, Lisztes E, Tóth-Molnár E, Nánási PP, Vennekens R, Tóth BI, and Almássy J
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- Animals, Calcium metabolism, Female, Ion Transport, Male, Mice, Mice, Inbred C57BL, Mice, Knockout, Pancreas, Exocrine cytology, Patch-Clamp Techniques, Phenanthrenes pharmacology, TRPM Cation Channels antagonists & inhibitors, TRPM Cation Channels genetics, Acinar Cells metabolism, Calcium Signaling, Membrane Potentials, Pancreas, Exocrine metabolism, TRPM Cation Channels metabolism
- Abstract
Transient receptor potential cation channel subfamily M member 4 (TRPM4) is a Ca
2+ -activated nonselective cation channel that mediates membrane depolarization. Although, a current with the hallmarks of a TRPM4-mediated current has been previously reported in pancreatic acinar cells (PACs), the role of TRPM4 in the regulation of acinar cell function has not yet been explored. In the present study, we identify this TRPM4 current and describe its role in context of Ca2+ signaling of PACs using pharmacological tools and TRPM4-deficient mice. We found a significant Ca2+ -activated cation current in PACs that was sensitive to the TRPM4 inhibitors 9-phenanthrol and 4-chloro-2-[[2-(2-chlorophenoxy)acetyl]amino]benzoic acid (CBA). We demonstrated that the CBA-sensitive current was responsible for a Ca2+ -dependent depolarization of PACs from a resting membrane potential of -44.4 ± 2.9 to -27.7 ± 3 mV. Furthermore, we showed that Ca2+ influx was higher in the TRPM4 KO- and CBA-treated PACs than in control cells. As hormone-induced repetitive Ca2+ transients partially rely on Ca2+ influx in PACs, the role of TRPM4 was also assessed on Ca2+ oscillations elicited by physiologically relevant concentrations of the cholecystokinin analog cerulein. These data show that the amplitude of Ca2+ signals was significantly higher in TRPM4 KO than in control PACs. Our results suggest that PACs are depolarized by TRPM4 currents to an extent that results in a significant reduction of the inward driving force for Ca2+ . In conclusion, TRPM4 links intracellular Ca2+ signaling to membrane potential as a negative feedback regulator of Ca2+ entry in PACs., Competing Interests: Conflict of interest The authors declare that they have no conflicts of interest with the contents of this article., (Copyright © 2021 The Authors. Published by Elsevier Inc. All rights reserved.)- Published
- 2021
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22. Electrophysiological Effects of the Transient Receptor Potential Melastatin 4 Channel Inhibitor (4-Chloro-2-(2-chlorophenoxy)acetamido) Benzoic Acid (CBA) in Canine Left Ventricular Cardiomyocytes.
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Dienes C, Hézső T, Kiss DZ, Baranyai D, Kovács ZM, Szabó L, Magyar J, Bányász T, Nánási PP, Horváth B, Gönczi M, and Szentandrássy N
- Subjects
- Action Potentials drug effects, Animals, Benzoic Acid pharmacology, Calcium metabolism, Cardiac Electrophysiology, Dogs, Electrophysiological Phenomena, Female, Heart Rate drug effects, Heart Ventricles pathology, Male, Myocytes, Cardiac metabolism, Patch-Clamp Techniques, Potassium metabolism, Sodium metabolism, TRPM Cation Channels antagonists & inhibitors, TRPM Cation Channels physiology, TRPM Cation Channels metabolism, Ventricular Function physiology
- Abstract
Transient receptor potential melastatin 4 (TRPM4) plays an important role in many tissues, including pacemaker and conductive tissues of the heart, but much less is known about its electrophysiological role in ventricular myocytes. Our earlier results showed the lack of selectivity of 9-phenanthrol, so CBA ((4-chloro-2-(2-chlorophenoxy)acetamido) benzoic acid) was chosen as a new, potentially selective inhibitor. Goal: Our aim was to elucidate the effect and selectivity of CBA in canine left ventricular cardiomyocytes and to study the expression of TRPM4 in the canine heart. Experiments were carried out in enzymatically isolated canine left ventricular cardiomyocytes. Ionic currents were recorded with an action potential (AP) voltage-clamp technique in whole-cell configuration at 37 °C. An amount of 10 mM BAPTA was used in the pipette solution to exclude the potential activation of TRPM4 channels. AP was recorded with conventional sharp microelectrodes. CBA was used in 10 µM concentrations. Expression of TRPM4 protein in the heart was studied by Western blot. TRPM4 protein was expressed in the wall of all four chambers of the canine heart as well as in samples prepared from isolated left ventricular cells. CBA induced an approximately 9% reduction in AP duration measured at 75% and 90% of repolarization and decreased the short-term variability of APD
90 . Moreover, AP amplitude was increased and the maximal rates of phase 0 and 1 were reduced by the drug. In AP clamp measurements, CBA-sensitive current contained a short, early outward and mainly a long, inward current. Transient outward potassium current (Ito ) and late sodium current (INa,L ) were reduced by approximately 20% and 47%, respectively, in the presence of CBA, while L-type calcium and inward rectifier potassium currents were not affected. These effects of CBA were largely reversible upon washout. Based on our results, the CBA induced reduction of phase-1 slope and the slight increase of AP amplitude could have been due to the inhibition of Ito . The tendency for AP shortening can be explained by the inhibition of inward currents seen in AP-clamp recordings during the plateau phase. This inward current reduced by CBA is possibly INa,L , therefore, CBA is not entirely selective for TRPM4 channels. As a consequence, similarly to 9-phenanthrol, it cannot be used to test the contribution of TRPM4 channels to cardiac electrophysiology in ventricular cells, or at least caution must be applied.- Published
- 2021
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23. Canine Myocytes Represent a Good Model for Human Ventricular Cells Regarding Their Electrophysiological Properties.
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Nánási PP, Horváth B, Tar F, Almássy J, Szentandrássy N, Jost N, Baczkó I, Bányász T, and Varró A
- Abstract
Due to the limited availability of healthy human ventricular tissues, the most suitable animal model has to be applied for electrophysiological and pharmacological studies. This can be best identified by studying the properties of ion currents shaping the action potential in the frequently used laboratory animals, such as dogs, rabbits, guinea pigs, or rats, and comparing them to those of human cardiomyocytes. The authors of this article with the experience of three decades of electrophysiological studies, performed in mammalian and human ventricular tissues and isolated cardiomyocytes, summarize their results obtained regarding the major canine and human cardiac ion currents. Accordingly, L-type Ca
2+ current (ICa ), late Na+ current (INa-late ), rapid and slow components of the delayed rectifier K+ current (IKr and IKs , respectively), inward rectifier K+ current (IK1 ), transient outward K+ current (Ito1 ), and Na+ /Ca2+ exchange current (INCX ) were characterized and compared. Importantly, many of these measurements were performed using the action potential voltage clamp technique allowing for visualization of the actual current profiles flowing during the ventricular action potential. Densities and shapes of these ion currents, as well as the action potential configuration, were similar in human and canine ventricular cells, except for the density of IK1 and the recovery kinetics of Ito . IK1 displayed a largely four-fold larger density in canine than human myocytes, and Ito recovery from inactivation displayed a somewhat different time course in the two species. On the basis of these results, it is concluded that canine ventricular cells represent a reasonably good model for human myocytes for electrophysiological studies, however, it must be borne in mind that due to their stronger IK1 , the repolarization reserve is more pronounced in canine cells, and moderate differences in the frequency-dependent repolarization patterns can also be anticipated.- Published
- 2021
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24. Mexiletine-like cellular electrophysiological effects of GS967 in canine ventricular myocardium.
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Hézső T, Naveed M, Dienes C, Kiss D, Prorok J, Árpádffy-Lovas T, Varga R, Fujii E, Mercan T, Topal L, Kistamás K, Szentandrássy N, Almássy J, Jost N, Magyar J, Bányász T, Baczkó I, Varró A, Nánási PP, Virág L, and Horváth B
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- Animals, Dogs, Female, Heart Rate drug effects, Male, Myocardium, Myocytes, Cardiac drug effects, Action Potentials drug effects, Anti-Arrhythmia Agents pharmacology, Heart drug effects, Mexiletine pharmacology, Pyridines pharmacology, Triazoles pharmacology
- Abstract
Enhancement of the late Na
+ current (INaL ) increases arrhythmia propensity in the heart, while suppression of the current is antiarrhythmic. GS967 is an agent considered as a selective blocker of INaL . In the present study, effects of GS967 on INaL and action potential (AP) morphology were studied in canine ventricular myocytes by using conventional voltage clamp, action potential voltage clamp and sharp microelectrode techniques. The effects of GS967 (1 µM) were compared to those of the class I/B antiarrhythmic compound mexiletine (40 µM). Under conventional voltage clamp conditions, INaL was significantly suppressed by GS967 and mexiletine, causing 80.4 ± 2.2% and 59.1 ± 1.8% reduction of the densities of INaL measured at 50 ms of depolarization, and 79.0 ± 3.1% and 63.3 ± 2.7% reduction of the corresponding current integrals, respectively. Both drugs shifted the voltage dependence of the steady-state inactivation curve of INaL towards negative potentials. GS967 and mexiletine dissected inward INaL profiles under AP voltage clamp conditions having densities, measured at 50% of AP duration (APD), of -0.37 ± 0.07 and -0.28 ± 0.03 A/F, and current integrals of -56.7 ± 9.1 and -46.6 ± 5.5 mC/F, respectively. Drug effects on peak Na+ current (INaP ) were assessed by recording the maximum velocity of AP upstroke (V+ max ) in multicellular preparations. The offset time constant was threefold faster for GS967 than mexiletine (110 ms versus 289 ms), while the onset of the rate-dependent block was slower in the case of GS967. Effects on beat-to-beat variability of APD was studied in isolated myocytes. Beat-to-beat variability was significantly decreased by both GS967 and mexiletine (reduction of 42.1 ± 6.5% and 24.6 ± 12.8%, respectively) while their shortening effect on APD was comparable. It is concluded that the electrophysiological effects of GS967 are similar to those of mexiletine, but with somewhat faster offset kinetics of V+ max block. However, since GS967 depressed V+ max and INaL at the same concentration, the current view that GS967 represents a new class of drugs that selectively block INaL has to be questioned and it is suggested that GS967 should be classified as a class I/B antiarrhythmic agent.- Published
- 2021
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25. Editorial: Perspectives of Antiarrhythmic Drug Therapy: Disappointing Past, Current Efforts, and Faint Hopes.
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Nánási PP, Pueyo E, and Virág L
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- 2020
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26. Late Sodium Current Inhibitors as Potential Antiarrhythmic Agents.
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Horváth B, Hézső T, Kiss D, Kistamás K, Magyar J, Nánási PP, and Bányász T
- Abstract
Based on recent findings, an increased late sodium current (I
Na,late ) plays an important pathophysiological role in cardiac diseases, including rhythm disorders. The article first describes what is INa,late and how it functions under physiological circumstances. Next, it shows the wide range of cellular mechanisms that can contribute to an increased INa,late in heart diseases, and also discusses how the upregulated INa,late can play a role in the generation of cardiac arrhythmias. The last part of the article is about INa,late inhibiting drugs as potential antiarrhythmic agents, based on experimental and preclinical data as well as in the light of clinical trials., (Copyright © 2020 Horváth, Hézső, Kiss, Kistamás, Magyar, Nánási and Bányász.)- Published
- 2020
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27. Calcium Handling Defects and Cardiac Arrhythmia Syndromes.
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Kistamás K, Veress R, Horváth B, Bányász T, Nánási PP, and Eisner DA
- Abstract
Calcium ions (Ca
2+ ) play a major role in the cardiac excitation-contraction coupling. Intracellular Ca2+ concentration increases during systole and falls in diastole thereby determining cardiac contraction and relaxation. Normal cardiac function also requires perfect organization of the ion currents at the cellular level to drive action potentials and to maintain action potential propagation and electrical homogeneity at the tissue level. Any imbalance in Ca2+ homeostasis of a cardiac myocyte can lead to electrical disturbances. This review aims to discuss cardiac physiology and pathophysiology from the elementary membrane processes that can cause the electrical instability of the ventricular myocytes through intracellular Ca2+ handling maladies to inherited and acquired arrhythmias. Finally, the paper will discuss the current therapeutic approaches targeting cardiac arrhythmias., (Copyright © 2020 Kistamás, Veress, Horváth, Bányász, Nánási and Eisner.)- Published
- 2020
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28. Handling of Ventricular Fibrillation in the Emergency Setting.
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Szabó Z, Ujvárosy D, Ötvös T, Sebestyén V, and Nánási PP
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Ventricular fibrillation (VF) and sudden cardiac death (SCD) are predominantly caused by channelopathies and cardiomyopathies in youngsters and coronary heart disease in the elderly. Temporary factors, e.g., electrolyte imbalance, drug interactions, and substance abuses may play an additive role in arrhythmogenesis. Ectopic automaticity, triggered activity, and reentry mechanisms are known as important electrophysiological substrates for VF determining the antiarrhythmic therapies at the same time. Emergency need for electrical cardioversion is supported by the fact that every minute without defibrillation decreases survival rates by approximately 7%-10%. Thus, early defibrillation is an essential part of antiarrhythmic emergency management. Drug therapy has its relevance rather in the prevention of sudden cardiac death, where early recognition and treatment of the underlying disease has significant importance. Cardioprotective and antiarrhythmic effects of beta blockers in patients predisposed to sudden cardiac death were highlighted in numerous studies, hence nowadays these drugs are considered to be the cornerstones of the prevention and treatment of life-threatening ventricular arrhythmias. Nevertheless, other medical therapies have not been proven to be useful in the prevention of VF. Although amiodarone has shown positive results occasionally, this was not demonstrated to be consistent. Furthermore, the potential proarrhythmic effects of drugs may also limit their applicability. Based on these unfavorable observations we highlight the importance of arrhythmia prevention, where echocardiography, electrocardiography and laboratory testing play a significant role even in the emergency setting. In the following we provide a summary on the latest developments on cardiopulmonary resuscitation, and the evaluation and preventive treatment possibilities of patients with increased susceptibility to VF and SCD., (Copyright © 2020 Szabó, Ujvárosy, Ötvös, Sebestyén and Nánási.)
- Published
- 2020
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29. Dantrolene Requires Mg 2+ and ATP To Inhibit the Ryanodine Receptor.
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Diszházi G, Magyar ZÉ, Mótyán JA, Csernoch L, Jóna I, Nánási PP, and Almássy J
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- Animals, Binding Sites, Calcium metabolism, Dantrolene chemistry, Male, Models, Molecular, Molecular Conformation, Muscle, Skeletal metabolism, Protein Binding, Rabbits, Ryanodine Receptor Calcium Release Channel chemistry, Adenosine Triphosphate metabolism, Dantrolene pharmacology, Magnesium metabolism, Ryanodine Receptor Calcium Release Channel metabolism
- Abstract
Dantrolene is a ryanodine receptor (RyR) inhibitor, which is used to relax muscles in malignant hyperthermia syndrome. Although dantrolene binds to the RyR protein, its mechanism of action is unknown, mainly because of the controversial results showing that dantrolene inhibited Ca
2+ release from intact fibers and sarcoplasmic reticulum (SR) vesicles, but failed to inhibit single RyR channel currents in bilayers. Accordingly, it was concluded that an important factor for dantrolene's action was lost during the purification procedure of RyR. Recently, Mg2+ was demonstrated to be the essential factor for dantrolene to inhibit Ca2+ release in skinned muscle fibers. The aim of the present study was to confirm these results in Ca2+ release and bilayer experiments, using SR vesicles and solubilized channels, respectively. Our Ca2+ release experiments demonstrated that the effect of dantrolene and Mg2+ was cooperative and that ATP enhanced the inhibiting effect of dantrolene. Namely, 10 µ M dantrolene reduced RyR channel open probability by ∼50% in the presence of 3 mM free Mg2+ and 1 mM ATP, whereas channel activity further decreased to ∼20% of control when [ATP] was increased to 2 mM. Our data provide important complementary information that supports the direct, Mg2+ -dependent mechanism of dantrolene's action and suggests that dantrolene also requires ATP to inhibit RyR., (Copyright © 2019 by The American Society for Pharmacology and Experimental Therapeutics.)- Published
- 2019
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30. Correction: Experimentally-Based Computational Investigation into Beat-To-Beat Variability in Ventricular Repolarization and Its Response to Ionic Current Inhibition.
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Pueyo E, Dangerfield CE, Britton OJ, Virág L, Kistamás K, Szentandrássy N, Jost N, Varró A, Nánási PP, Burrage K, and Rodríguez B
- Abstract
[This corrects the article DOI: 10.1371/journal.pone.0151461.].
- Published
- 2018
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31. Activation of TRPV3 Regulates Inflammatory Actions of Human Epidermal Keratinocytes.
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Szöllősi AG, Vasas N, Angyal Á, Kistamás K, Nánási PP, Mihály J, Béke G, Herczeg-Lisztes E, Szegedi A, Kawada N, Yanagida T, Mori T, Kemény L, and Bíró T
- Subjects
- Calcium metabolism, Cations, Divalent metabolism, Cell Proliferation, Dermatitis pathology, Epidermis metabolism, HEK293 Cells, Healthy Volunteers, Humans, Keratinocytes metabolism, TRPV Cation Channels metabolism, Apoptosis immunology, Dermatitis immunology, Epidermis immunology, Keratinocytes immunology, TRPV Cation Channels immunology
- Abstract
Transient receptor potential (TRP) ion channels were first characterized on neurons, where they are classically implicated in sensory functions; however, research in recent decades has shown that many of these channels are also expressed on nonneuronal cell types. Emerging findings have highlighted the role of TRP channels in the skin, where they have been shown to be important in numerous cutaneous functions. Of particular interest is TRPV3, which was first described on keratinocytes. Its functional importance was supported when its gain-of-function mutation was linked to Olmsted syndrome, which is characterized by palmoplantar keratoderma, periorifacial hyperkeratosis, diffuse hypotrichosis and alopecia, and itch. Despite these exciting results, we have no information about the role and functionality of TRPV3 on keratinocytes at the cellular level. In this study, we identified TRPV3 expression both on human skin and cultured epidermal keratinocytes. TRPV3 stimulation was found to function as a Ca
2+ -permeable ion channel that suppresses proliferation of epidermal keratinocytes and induces cell death. Stimulation of the channel also triggers a strong proinflammatory response via the NF-κB pathway. Collectively, our data show that TRPV3 is functionally expressed on human epidermal keratinocytes and that it plays a role in cutaneous inflammatory processes., (Copyright © 2017 The Authors. Published by Elsevier Inc. All rights reserved.)- Published
- 2018
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32. The Effect of a Novel Highly Selective Inhibitor of the Sodium/Calcium Exchanger (NCX) on Cardiac Arrhythmias in In Vitro and In Vivo Experiments.
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Kohajda Z, Farkas-Morvay N, Jost N, Nagy N, Geramipour A, Horváth A, Varga RS, Hornyik T, Corici C, Acsai K, Horváth B, Prorok J, Ördög B, Déri S, Tóth D, Levijoki J, Pollesello P, Koskelainen T, Otsomaa L, Tóth A, Baczkó I, Leprán I, Nánási PP, Papp JG, Varró A, and Virág L
- Subjects
- Animals, Anti-Arrhythmia Agents pharmacology, Arrhythmias, Cardiac metabolism, Arrhythmias, Cardiac pathology, Calcium metabolism, Cells, Cultured, Dogs, Drug Discovery, Guinea Pigs, Heart Ventricles drug effects, Heart Ventricles metabolism, Heart Ventricles pathology, Male, Myocytes, Cardiac drug effects, Myocytes, Cardiac metabolism, Myocytes, Cardiac pathology, Rats, Sprague-Dawley, Sodium-Calcium Exchanger metabolism, Action Potentials drug effects, Anti-Arrhythmia Agents chemistry, Anti-Arrhythmia Agents therapeutic use, Arrhythmias, Cardiac drug therapy, Sodium-Calcium Exchanger antagonists & inhibitors
- Abstract
Background: In this study the effects of a new, highly selective sodium-calcium exchanger (NCX) inhibitor, ORM-10962 were investigated on cardiac NCX current, Ca2+ transients, cell shortening and in experimental arrhythmias. The level of selectivity of the novel inhibitor on several major transmembrane ion currents (L-type Ca2+ current, major repolarizing K+ currents, late Na+ current, Na+/K+ pump current) was also determined., Methods: Ion currents in single dog ventricular cells (cardiac myocytes; CM), and action potentials in dog cardiac multicellular preparations were recorded utilizing the whole-cell patch clamp and standard microelectrode techniques, respectively. Ca2+ transients and cell shortening were measured in fluorescent dye loaded isolated dog myocytes. Antiarrhythmic effects of ORM-10962 were studied in anesthetized ouabain (10 μg/kg/min i.v.) pretreated guinea pigs and in ischemia-reperfusion models (I/R) of anesthetized coronary artery occluded rats and Langendorff perfused guinea pigs hearts., Results: ORM-10962 significantly reduced the inward/outward NCX currents with estimated EC50 values of 55/67 nM, respectively. The compound, even at a high concentration of 1 μM, did not modify significantly the magnitude of ICaL in CMs, neither had any apparent influence on the inward rectifier, transient outward, the rapid and slow components of the delayed rectifier potassium currents, the late and peak sodium and Na+/K+ pump currents. NCX inhibition exerted moderate positive inotropic effect under normal condition, negative inotropy when reverse, and further positive inotropic effect when forward mode was facilitated. In dog Purkinje fibres 1 μM ORM-10962 decreased the amplitude of digoxin induced delayed afterdepolarizations (DADs). Pre-treatment with 0.3 mg/kg ORM-10962 (i.v.) 10 min before starting ouabain infusion significantly delayed the development and recurrence of ventricular extrasystoles (by about 50%) or ventricular tachycardia (by about 30%) in anesthetized guinea pigs. On the contrary, ORM-10962 pre-treatment had no apparent influence on the time of onset or the severity of I/R induced arrhythmias in anesthetized rats and in Langendorff perfused guinea-pig hearts., Conclusions: The present study provides strong evidence for a high efficacy and selectivity of the NCX-inhibitory effect of ORM-10962. Selective NCX inhibition can exert positive as well as negative inotropic effect depending on the actual operation mode of NCX. Selective NCX blockade may contribute to the prevention of DAD based arrhythmogenesis, in vivo, however, its effect on I/R induced arrhythmias is still uncertain., Competing Interests: T. Koskaleinen, J. Levijoki, L. Otsomaa and P. Pollesello, employed by Orion Pharma, have been involved in the development of ORM-10962. Other authors have nothing to declare. The commercial adherence of the Orion Pharma does not alter our adherence to PLOS ONE policies on sharing data and materials.
- Published
- 2016
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33. Experimentally-Based Computational Investigation into Beat-To-Beat Variability in Ventricular Repolarization and Its Response to Ionic Current Inhibition.
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Pueyo E, Dangerfield CE, Britton OJ, Virág L, Kistamás K, Szentandrássy N, Jost N, Varró A, Nánási PP, Burrage K, and Rodríguez B
- Subjects
- 4-Aminopyridine pharmacology, Action Potentials drug effects, Algorithms, Animals, Calcium Channels, L-Type physiology, Cells, Cultured, Computer Simulation, Dogs, Humans, Ion Channel Gating drug effects, Kinetics, Models, Cardiovascular, Myocytes, Cardiac cytology, Myocytes, Cardiac drug effects, Potassium Channel Blockers pharmacology, Stochastic Processes, Action Potentials physiology, Computational Biology methods, Ion Channel Gating physiology, Myocytes, Cardiac physiology, Potassium Channels physiology
- Abstract
Beat-to-beat variability in repolarization (BVR) has been proposed as an arrhythmic risk marker for disease and pharmacological action. The mechanisms are unclear but BVR is thought to be a cell level manifestation of ion channel stochasticity, modulated by cell-to-cell differences in ionic conductances. In this study, we describe the construction of an experimentally-calibrated set of stochastic cardiac cell models that captures both BVR and cell-to-cell differences in BVR displayed in isolated canine action potential measurements using pharmacological agents. Simulated and experimental ranges of BVR are compared in control and under pharmacological inhibition, and the key ionic currents determining BVR under physiological and pharmacological conditions are identified. Results show that the 4-aminopyridine-sensitive transient outward potassium current, Ito1, is a fundamental driver of BVR in control and upon complete inhibition of the slow delayed rectifier potassium current, IKs. In contrast, IKs and the L-type calcium current, ICaL, become the major contributors to BVR upon inhibition of the fast delayed rectifier potassium current, IKr. This highlights both IKs and Ito1 as key contributors to repolarization reserve. Partial correlation analysis identifies the distribution of Ito1 channel numbers as an important independent determinant of the magnitude of BVR and drug-induced change in BVR in control and under pharmacological inhibition of ionic currents. Distributions in the number of IKs and ICaL channels only become independent determinants of the magnitude of BVR upon complete inhibition of IKr. These findings provide quantitative insights into the ionic causes of BVR as a marker for repolarization reserve, both under control condition and pharmacological inhibition.
- Published
- 2016
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34. Selective Na(+) /Ca(2+) exchanger inhibition prevents Ca(2+) overload-induced triggered arrhythmias.
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Nagy N, Kormos A, Kohajda Z, Szebeni Á, Szepesi J, Pollesello P, Levijoki J, Acsai K, Virág L, Nánási PP, Papp JG, Varró A, and Tóth A
- Subjects
- Animals, Arrhythmias, Cardiac etiology, Cnidarian Venoms pharmacology, Dogs, Hypercalcemia complications, Myocytes, Cardiac metabolism, Papillary Muscles metabolism, Patch-Clamp Techniques, Purkinje Fibers metabolism, Action Potentials drug effects, Aniline Compounds pharmacology, Benzopyrans pharmacology, Calcium metabolism, Myocytes, Cardiac drug effects, Papillary Muscles drug effects, Phenyl Ethers pharmacology, Purkinje Fibers drug effects, Pyridines pharmacology, Sodium-Calcium Exchanger antagonists & inhibitors
- Abstract
Background and Purpose: Augmented Na(+) /Ca(2+) exchanger (NCX) activity may play a crucial role in cardiac arrhythmogenesis; however, data regarding the anti-arrhythmic efficacy of NCX inhibition are debatable. Feasible explanations could be the unsatisfactory selectivity of NCX inhibitors and/or the dependence of the experimental model on the degree of Ca(2+) i overload. Hence, we used NCX inhibitors SEA0400 and the more selective ORM10103 to evaluate the efficacy of NCX inhibition against arrhythmogenic Ca(2+) i rise in conditions when [Ca(2+) ]i was augmented via activation of the late sodium current (INaL ) or inhibition of the Na(+) /K(+) pump., Experimental Approach: Action potentials (APs) were recorded from canine papillary muscles and Purkinje fibres by microelectrodes. NCX current (INCX ) was determined in ventricular cardiomyocytes utilizing the whole-cell patch clamp technique. Ca(2+) i transients (CaTs) were monitored with a Ca(2+) -sensitive fluorescent dye, Fluo-4., Key Results: Enhanced INaL increased the Ca(2+) load and AP duration (APD). SEA0400 and ORM10103 suppressed INCX and prevented/reversed the anemone toxin II (ATX-II)-induced [Ca(2+) ]i rise without influencing APD, CaT or cell shortening, or affecting the ATX-II-induced increased APD. ORM10103 significantly decreased the number of strophanthidin-induced spontaneous diastolic Ca(2+) release events; however, SEA0400 failed to restrict the veratridine-induced augmentation in Purkinje-ventricle APD dispersion., Conclusions and Implications: Selective NCX inhibition - presumably by blocking rev INCX (reverse mode NCX current) - is effective against arrhythmogenesis caused by [Na(+) ]i -induced [Ca(2+) ]i elevation, without influencing the AP waveform. Therefore, selective INCX inhibition, by significantly reducing the arrhythmogenic trigger activity caused by the perturbed Ca(2+) i handling, should be considered as a promising anti-arrhythmic therapeutic strategy., (© 2014 The British Pharmacological Society.)
- Published
- 2014
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35. Ionic mechanisms limiting cardiac repolarization reserve in humans compared to dogs.
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Jost N, Virág L, Comtois P, Ordög B, Szuts V, Seprényi G, Bitay M, Kohajda Z, Koncz I, Nagy N, Szél T, Magyar J, Kovács M, Puskás LG, Lengyel C, Wettwer E, Ravens U, Nánási PP, Papp JG, Varró A, and Nattel S
- Subjects
- Adult, Animals, Calcium metabolism, Cells, Cultured, Dogs, Female, Humans, Ion Transport, Male, Middle Aged, Myocytes, Cardiac metabolism, Potassium Channels genetics, Potassium Channels metabolism, RNA, Messenger genetics, RNA, Messenger metabolism, Sodium metabolism, Sodium-Calcium Exchanger metabolism, Species Specificity, Action Potentials, Heart physiology, Models, Cardiovascular, Myocytes, Cardiac physiology, Potassium metabolism
- Abstract
The species-specific determinants of repolarization are poorly understood. This study compared the contribution of various currents to cardiac repolarization in canine and human ventricle. Conventional microelectrode, whole-cell patch-clamp, molecular biological and mathematical modelling techniques were used. Selective IKr block (50-100 nmol l(-1) dofetilide) lengthened AP duration at 90% of repolarization (APD90) >3-fold more in human than dog, suggesting smaller repolarization reserve in humans. Selective IK1 block (10 μmol l(-1) BaCl2) and IKs block (1 μmol l(-1) HMR-1556) increased APD90 more in canine than human right ventricular papillary muscle. Ion current measurements in isolated cardiomyocytes showed that IK1 and IKs densities were 3- and 4.5-fold larger in dogs than humans, respectively. IKr density and kinetics were similar in human versus dog. ICa and Ito were respectively ~30% larger and ~29% smaller in human, and Na(+)-Ca(2+) exchange current was comparable. Cardiac mRNA levels for the main IK1 ion channel subunit Kir2.1 and the IKs accessory subunit minK were significantly lower, but mRNA expression of ERG and KvLQT1 (IKr and IKs α-subunits) were not significantly different, in human versus dog. Immunostaining suggested lower Kir2.1 and minK, and higher KvLQT1 protein expression in human versus canine cardiomyocytes. IK1 and IKs inhibition increased the APD-prolonging effect of IKr block more in dog (by 56% and 49%, respectively) than human (34 and 16%), indicating that both currents contribute to increased repolarization reserve in the dog. A mathematical model incorporating observed human-canine ion current differences confirmed the role of IK1 and IKs in repolarization reserve differences. Thus, humans show greater repolarization-delaying effects of IKr block than dogs, because of lower repolarization reserve contributions from IK1 and IKs, emphasizing species-specific determinants of repolarization and the limitations of animal models for human disease.
- Published
- 2013
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36. Tetrodotoxin blockade on canine cardiac L-type Ca²⁺ channels depends on pH and redox potential.
- Author
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Hegyi B, Komáromi I, Kistamás K, Ruzsnavszky F, Váczi K, Horváth B, Magyar J, Bányász T, Nánási PP, and Szentandrássy N
- Subjects
- Animals, Calcium Channel Blockers administration & dosage, Calcium Channels, L-Type metabolism, Dogs, Hydrogen-Ion Concentration, Inhibitory Concentration 50, Myocytes, Cardiac metabolism, Oxidation-Reduction, Patch-Clamp Techniques, Phosphorylation, Tetrodotoxin administration & dosage, Calcium Channel Blockers pharmacology, Calcium Channels, L-Type drug effects, Myocytes, Cardiac drug effects, Tetrodotoxin pharmacology
- Abstract
Tetrodotoxin (TTX) is believed to be one of the most selective inhibitors of voltage-gated fast Na⁺ channels in excitable tissues. Recently, however, TTX has been shown to block L-type Ca²⁺ current (I(Ca)) in canine cardiac cells. In the present study, the TTX-sensitivity of I(Ca) was studied in isolated canine ventricular myocytes as a function of (1) channel phosphorylation, (2) extracellular pH and (3) the redox potential of the bathing medium using the whole cell voltage clamp technique. Fifty-five micromoles of TTX (IC₅₀ value obtained under physiological conditions) caused 60% ± 2% inhibition of I(Ca) in acidic (pH = 6.4), while only a 26% ± 2% block in alkaline (pH = 8.4) milieu. Similarly, the same concentration of TTX induced 62% ± 6% suppression of ICa in a reductant milieu (containing glutathione + ascorbic acid + dithiothreitol, 1 mM each), in contrast to the 31% ± 3% blockade obtained in the presence of a strong oxidant (100 μM H₂O₂). Phosphorylation of the channel protein (induced by 3 μM forskolin) failed to modify the inhibiting potency of TTX; an IC₅₀ value of 50 ± 4 μM was found in forskolin. The results are in a good accordance with the predictions of our model, indicating that TTX binds, in fact, to the selectivity filter of cardiac L-type Ca channels.
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- 2013
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37. A multiscale investigation of repolarization variability and its role in cardiac arrhythmogenesis.
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Pueyo E, Corrias A, Virág L, Jost N, Szél T, Varró A, Szentandrássy N, Nánási PP, Burrage K, and Rodríguez B
- Subjects
- Animals, Computer Simulation, Dogs, Guinea Pigs, Humans, Species Specificity, Action Potentials, Arrhythmias, Cardiac physiopathology, Heart Conduction System physiopathology, Heart Rate, Models, Cardiovascular, Myocytes, Cardiac
- Abstract
Enhanced temporal and spatial variability in cardiac repolarization has been related to increased arrhythmic risk both clinically and experimentally. Causes and modulators of variability in repolarization and their implications in arrhythmogenesis are however not well understood. At the ionic level, the slow component of the delayed rectifier potassium current (I(Ks)) is an important determinant of ventricular repolarization. In this study, a combination of experimental and computational multiscale studies is used to investigate the role of intrinsic and extrinsic noise in I(Ks) in modulating temporal and spatial variability in ventricular repolarization in human and guinea pig. Results show that under physiological conditions: i), stochastic fluctuations in I(Ks) gating properties (i.e., intrinsic noise) cause significant beat-to-beat variability in action potential duration (APD) in isolated cells, whereas cell-to-cell differences in channel numbers (i.e., extrinsic noise) also contribute to cell-to-cell APD differences; ii), in tissue, electrotonic interactions mask the effect of I(Ks) noise, resulting in a significant decrease in APD temporal and spatial variability compared to isolated cells. Pathological conditions resulting in gap junctional uncoupling or a decrease in repolarization reserve uncover the manifestation of I(Ks) noise at cellular and tissue level, resulting in enhanced ventricular variability and abnormalities in repolarization such as afterdepolarizations and alternans., (Copyright © 2011 Biophysical Society. Published by Elsevier Inc. All rights reserved.)
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- 2011
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38. Activation of transient receptor potential vanilloid-3 inhibits human hair growth.
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Borbíró I, Lisztes E, Tóth BI, Czifra G, Oláh A, Szöllosi AG, Szentandrássy N, Nánási PP, Péter Z, Paus R, Kovács L, and Bíró T
- Subjects
- Alopecia pathology, Alopecia physiopathology, Anti-Infective Agents pharmacology, Apoptosis drug effects, Apoptosis physiology, Boron Compounds pharmacology, Calcium metabolism, Cell Division drug effects, Cell Division physiology, Cells, Cultured, Eugenol pharmacology, Female, Hair growth & development, Humans, Keratinocytes cytology, Keratinocytes drug effects, Keratinocytes physiology, Male, Organ Culture Techniques, Patch-Clamp Techniques, Scalp cytology, Alopecia drug therapy, Hair drug effects, Hair Follicle cytology, Hair Follicle drug effects, Hair Follicle physiology, TRPV Cation Channels agonists, TRPV Cation Channels physiology
- Abstract
In the current study, we aimed at identifying the functional role of transient receptor potential vanilloid-3 (TRPV3) ion channel in the regulation of human hair growth. Using human organ-cultured hair follicles (HFs) and cultures of human outer root sheath (ORS) keratinocytes, we provide the first evidence that activation of TRPV3 inhibits human hair growth. TRPV3 immunoreactivity was confined to epithelial compartments of the human HF, mainly to the ORS. In organ culture, TRPV3 activation by plant-derived (e.g., eugenol, 10-1,000 μM) or synthetic (e.g., 2-aminoethoxydiphenyl borate, 1-300 μM) agonists resulted in a dose-dependent inhibition of hair shaft elongation, suppression of proliferation, and induction of apoptosis and premature HF regression (catagen). Human ORS keratinocytes also expressed functional TRPV3, whose stimulation induced membrane currents, elevated intracellular calcium concentration, inhibited proliferation, and induced apoptosis. Of great importance, these effects on ORS keratinocytes were all mediated by TRPV3, as small interfering RNA-mediated silencing of TRPV3 effectively abrogated the cellular actions of the above agonists. These findings collectively support the concept that TRPV3 signaling is a significant player in human hair growth control. Therefore, TRPV3 and the related intracellular signaling mechanism might function as a promising target for pharmacological manipulations of clinically relevant hair growth disorders.
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- 2011
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39. Reverse rate dependency is an intrinsic property of canine cardiac preparations.
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Bányász T, Horváth B, Virág L, Bárándi L, Szentandrássy N, Harmati G, Magyar J, Marangoni S, Zaza A, Varró A, and Nánási PP
- Subjects
- 3-Pyridinecarboxylic acid, 1,4-dihydro-2,6-dimethyl-5-nitro-4-(2-(trifluoromethyl)phenyl)-, Methyl ester pharmacology, Action Potentials, Animals, Barium Compounds pharmacology, Cardiac Pacing, Artificial, Chlorides pharmacology, Computer Simulation, Dogs, Female, Heart Ventricles cytology, Heart Ventricles drug effects, In Vitro Techniques, Male, Models, Cardiovascular, Myocytes, Cardiac physiology, Nonlinear Dynamics, Phenethylamines pharmacology, Purkinje Fibers physiology, Sulfonamides pharmacology, Time Factors, Veratrine pharmacology, Anti-Arrhythmia Agents pharmacology, Myocytes, Cardiac drug effects, Purkinje Fibers drug effects
- Abstract
Aims: Class III antiarrhythmic agents exhibit reverse rate-dependent lengthening of the action potential duration (APD). In spite of the several theories developed so far to explain this reverse rate dependency (RRD), its mechanism has not yet been clarified. The aim of the present work was to further elucidate the mechanisms responsible for reverse rate-dependent drug effects., Methods and Results: Action potentials were recorded from multicellular canine ventricular preparations and isolated cardiomyocytes, at cycle lengths (CLs) varying from 0.3 to 5 s, using conventional sharp microelectrodes. APD was either modified by applying inward and outward current pulses, or by superfusion of agents known to lengthen and shorten APD. Net membrane current (I(m)) was calculated from action potential waveforms. The hypothesis that RRD may be implicit in the relationship between I(m) and APD was tested by numerical modelling. Both drug-induced lengthening (by veratrine, BAY-K 8644, dofetilide, and BaCl(2)) and shortening (by lidocaine and nicorandil) of action potentials displayed RRD, i.e. changes in APD were greater at longer than at shorter CL. A similar dependency of effect on CL was found when repolarization was modified by injection of inward or outward current pulses. I(m) measured at various points during repolarization was inversely proportional to APD and to CL. Model simulations showed that RRD is expected as a consequence of the non-linearity of the relationship between I(m) and APD., Conclusion: RRD of APD modulation is shared, although with differences in magnitude, by interventions of very different nature. RRD can be interpreted as a consequence of the relationship between I(m) and APD and, as such, is expected in all species having positive APD-CL relationship. This implies that the development of agents prolonging APD with direct rate dependency, or even completely devoid of RRD, may be difficult to achieve.
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- 2009
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40. The Na+/Ca2+ exchange blocker SEA0400 fails to enhance cytosolic Ca2+ transient and contractility in canine ventricular cardiomyocytes.
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Birinyi P, Tóth A, Jóna I, Acsai K, Almássy J, Nagy N, Prorok J, Gherasim I, Papp Z, Hertelendi Z, Szentandrássy N, Bányász T, Fülöp F, Papp JG, Varró A, Nánási PP, and Magyar J
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- Animals, Caffeine pharmacology, Calcium Channels, L-Type metabolism, Cardiac Pacing, Artificial, Cell Size drug effects, Cytosol metabolism, Dogs, Dose-Response Relationship, Drug, Female, In Vitro Techniques, Ion Channel Gating drug effects, Male, Membrane Potentials, Myocytes, Cardiac metabolism, Patch-Clamp Techniques, Ryanodine Receptor Calcium Release Channel drug effects, Ryanodine Receptor Calcium Release Channel metabolism, Sarcoplasmic Reticulum drug effects, Sarcoplasmic Reticulum metabolism, Sodium-Calcium Exchanger metabolism, Time Factors, Aniline Compounds pharmacology, Calcium Signaling drug effects, Myocardial Contraction drug effects, Myocytes, Cardiac drug effects, Phenyl Ethers pharmacology, Sodium-Calcium Exchanger antagonists & inhibitors
- Abstract
Aims: This study was designed to evaluate the effects of the Na(+)/Ca(2+) exchange (NCX) inhibitor SEA0400 on Ca(2+) handling in isolated canine ventricular myocytes., Methods and Results: Intracellular Ca(2+) ([Ca(2+)](i)) transients, induced by either field stimulation or caffeine flush, were monitored using Ca(2+) indicator dyes. [Ca(2+)](i)-dependent modulation of the inhibitory effect of SEA0400 on NCX was characterized by the changes in Ni(2+)-sensitive current in voltage-clamped myocytes. Sarcoplasmic reticulum (SR) Ca(2+) release and uptake were studied in SR membrane vesicles. Gating properties of single-ryanodine receptors were analysed in lipid bilayers. Ca(2+) sensitivity of the contractile machinery was evaluated in chemically skinned myocytes. In myocytes paced at 1 Hz, neither diastolic [Ca(2+)](i) nor the amplitude of [Ca(2+)](i) transients was significantly altered by SEA0400 up to the concentration of 1 microM, which was shown to inhibit the exchange current. The blocking effect of SEA0400 on NCX decreased with increasing [Ca(2+)](i), and it was more pronounced in reverse than in forward mode operation at every [Ca(2+)](i) examined. The rate of decay of the caffeine-induced [Ca(2+)](i) transients was decreased significantly by 1 microM SEA0400; however, this effect was only a fraction of that observed with 10 mM NiCl(2). Neither SR Ca(2+) release and uptake nor cell shortening and Ca(2+) sensitivity of the contractile proteins were influenced by SEA0400., Conclusion: The lack of any major SEA0400-induced shift in Ca(2+) transients or contractility of myocytes can well be explained by its limited inhibitory effect on NCX (further attenuated by elevated [Ca(2+)](i) levels) and a concomitant reduction in Ca(2+) influx due to the predominantly reverse mode blockade of NCX and suppression of L-type Ca(2+) current.
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- 2008
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41. Na(+)/Ca(2+) exchanger inhibition exerts a positive inotropic effect in the rat heart, but fails to influence the contractility of the rabbit heart.
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Farkas AS, Acsai K, Nagy N, Tóth A, Fülöp F, Seprényi G, Birinyi P, Nánási PP, Forster T, Csanády M, Papp JG, Varró A, and Farkas A
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- Action Potentials drug effects, Aniline Compounds pharmacology, Animals, Blood Pressure drug effects, Coronary Circulation drug effects, Electrocardiography drug effects, Heart Rate drug effects, Immunohistochemistry, Microscopy, Confocal, Myocytes, Cardiac drug effects, Patch-Clamp Techniques, Phenyl Ethers pharmacology, Rabbits, Rats, Species Specificity, Cardiotonic Agents pharmacology, Heart drug effects, Myocardial Contraction drug effects, Sodium-Calcium Exchanger antagonists & inhibitors
- Abstract
Background and Purpose: The Na(+)/Ca(2+) exchanger (NCX) may play a key role in myocardial contractility. The operation of the NCX is affected by the action potential (AP) configuration and the intracellular Na(+) concentration. This study examined the effect of selective NCX inhibition by 0.1, 0.3 and 1.0 microM SEA0400 on the myocardial contractility in the setting of different AP configurations and different intracellular Na(+) concentrations in rabbit and rat hearts., Experimental Approach: The concentration-dependent effects of SEA0400 on I(Na/Ca) were studied in rat and rabbit ventricular cardiomyocytes using a patch clamp technique. Starling curves were constructed for isolated, Langendorff-perfused rat and rabbit hearts. The cardiac sarcolemmal NCX protein densities of both species were compared by immunohistochemistry., Key Results: SEA0400 inhibited I(Na/Ca) with similar efficacy in the two species; there was no difference between the inhibitions of the forward or reverse mode of the NCX in either species. SEA0400 increased the systolic and the developed pressure in the rat heart in a concentration-dependent manner, for example, 1.0 microM SEA0400 increased the maximum systolic pressures by 12% relative to the control, whereas it failed to alter the contractility in the rabbit heart. No interspecies difference was found in the cardiac sarcolemmal NCX protein densities., Conclusions and Implications: NCX inhibition exerted a positive inotropic effect in the rat heart, but it did not influence the contractility of the rabbit heart. This implies that the AP configuration and the intracellular Na(+) concentration may play an important role in the contractility response to NCX inhibition.
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- 2008
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42. Effects of ropivacaine on action potential configuration and ion currents in isolated canine ventricular cardiomyocytes.
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Szabó A, Szentandrássy N, Birinyi P, Horváth B, Szabó G, Bányász T, Márton I, Magyar J, and Nánási PP
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- Action Potentials physiology, Animals, Dogs, Dose-Response Relationship, Drug, Female, Heart Ventricles cytology, Heart Ventricles drug effects, Ion Transport drug effects, Ion Transport physiology, Male, Myocytes, Cardiac physiology, Ropivacaine, Ventricular Function, Action Potentials drug effects, Amides pharmacology, Ion Channels physiology, Myocytes, Cardiac drug effects
- Abstract
Background: Despite the widespread clinical application of ropivacaine, there is little information on the cellular cardiac effects of the drug. In the current study, therefore, the concentration-dependent effects of ropivacaine on action potential morphology and the underlying ion currents were studied and compared with those of bupivacaine in isolated canine ventricular cardiomyocytes., Methods: Action potentials were recorded from the enzymatically dispersed cells using sharp microelectrodes. Conventional patch clamp and action potential voltage clamp arrangements were used to study the effects of ropivacaine on transmembrane ion currents., Results: Ropivacaine induced concentration- and frequency-dependent changes in action potential configuration, including shortening of the action potentials, reduction of their amplitude and maximum velocity of depolarization, suppression of early repolarization, and depression of plateau. Reduction in maximum velocity of depolarization was characterized with an EC50 value of 81 +/- 7 microm at 1 Hz. Qualitatively similar results were obtained with bupivacaine (EC50 = 47 +/- 3 microm). Under voltage clamp conditions, a variety of ion currents were blocked by ropivacaine: L-type calcium current (EC50 = 263 +/- 67 microm), transient outward current (EC50 = 384 +/- 75 microm), inward rectifier potassium current (EC50 = 372 +/- 35 microm), rapid delayed rectifier potassium current (EC50 = 303 +/- 47 microm), and slow delayed rectifier potassium current (EC50 = 106 +/- 18 microm)., Conclusions: Ropivacaine, similarly to bupivacaine, can modify cardiac action potentials and the underlying ion currents at concentrations higher than the usual therapeutic range. However, in cases of overdose, cardiac complications may be anticipated both during and after anesthesia due to the blockade of various ion currents.
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- 2008
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43. Effects of articaine on action potential characteristics and the underlying ion currents in canine ventricular myocytes.
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Szabó A, Szentandrássy N, Birinyi P, Horváth B, Szabó G, Bányász T, Márton I, Nánási PP, and Magyar J
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- Animals, Calcium Channels drug effects, Calcium Channels metabolism, Cells, Cultured, Dogs, Dose-Response Relationship, Drug, Female, Heart Ventricles drug effects, Heart Ventricles metabolism, Male, Microelectrodes, Myocytes, Cardiac metabolism, Myocytes, Cardiac physiology, Patch-Clamp Techniques, Potassium Channels drug effects, Potassium Channels metabolism, Action Potentials drug effects, Anesthetics, Local pharmacology, Carticaine pharmacology, Myocytes, Cardiac drug effects
- Abstract
Background: In spite of its widespread clinical application, there is little information on the cellular cardiac effects of articaine. In the present study, the concentration-dependent effects of articaine on action potential morphology and the underlying ion currents were studied in isolated canine ventricular cardiomyocytes., Methods: Action potentials were recorded from the enzymatically dispersed myocytes using sharp microelectrodes (16 cells from 3 dogs). Conventional patch clamp and action potential voltage clamp arrangements were used to study the effects of articaine on transmembrane ion currents (37 cells from 14 dogs)., Results: Articaine-induced concentration-dependent changes in action potential configuration including shortening of the action potentials, reduction of their amplitude and maximum velocity of depolarization (V(max)), suppression of early repolarization and depression of plateau. The EC50 value obtained for the V(max) block was 162 (sd 30) microM. Both the reduction of V(max) and action potential shortening were frequency dependent: the former was more prominent at shorter, while the latter at longer pacing cycle lengths. A rate dependent V(max) block, having rapid offset kinetics [tau = 91 (20) ms], was observed in addition to tonic block. Under voltage clamp conditions, a variety of ion currents were blocked by articaine: I(Ca) [EC50 = 471 (75) microM], I(to) [EC50 = 365 (62) microM], I(K1) [EC50 = 372 (46) microM], I(Kr) [EC50 = 278 (79) microM], and I(Ks) [EC50 = 326 (65) microM]. Hill coefficients were close to unity indicating a single binding site for articaine, except for I(K1)., Conclusions: Articaine can modify cardiac action potentials and ion currents at concentrations higher than the therapeutic range which can be achieved only by accidental venous injection. Since its suppressive effects on the inward and outward currents are relatively well balanced, the articaine-induced changes in action potential morphology may be moderate even in the case of overdose.
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- 2007
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44. Diabetes mellitus attenuates the repolarization reserve in mammalian heart.
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Lengyel C, Virág L, Bíró T, Jost N, Magyar J, Biliczki P, Kocsis E, Skoumal R, Nánási PP, Tóth M, Kecskeméti V, Papp JG, and Varró A
- Subjects
- Action Potentials, Animals, Blotting, Western methods, Calcium Channels, L-Type metabolism, Delayed Rectifier Potassium Channels metabolism, Diabetes Mellitus, Experimental, Diabetes Mellitus, Type 1 drug therapy, Dogs, Electrocardiography, Female, Insulin therapeutic use, KCNQ1 Potassium Channel metabolism, Kv1.4 Potassium Channel metabolism, Male, Patch-Clamp Techniques, Potassium Channels, Inwardly Rectifying metabolism, Shal Potassium Channels metabolism, Diabetes Mellitus, Type 1 metabolism, Diabetes Mellitus, Type 1 physiopathology, Myocardium metabolism
- Abstract
Objective: In diabetes mellitus several cardiac electrophysiological parameters are known to be affected. In rodent experimental diabetes models changes in these parameters were reported, but no such data are available in other mammalian species including the dog. The present study was designed to analyse the effects of experimental type 1 diabetes on ventricular repolarization and its underlying transmembrane ionic currents and channel proteins in canine hearts., Methods and Results: Diabetes was induced by a single injection of alloxan, a subgroup of dogs received insulin substitution. After the development of diabetes (8 weeks) electrophysiological studies were performed using conventional microelectrodes, whole cell voltage clamp, and ECG. Expression of ion channel proteins was evaluated by Western blotting. The QTc interval and the ventricular action potential duration in diabetic dogs were moderately prolonged. This was accompanied by significant reduction in the density of the transient outward K+ current (I(to)) and the slow delayed rectifier K+ current (I(Ks)), to 54.6% and 69.3% of control, respectively. No differences were observed in the density of the inward rectifier K+ current (I(K1)), rapid delayed rectifier K+ current (I(Kr)), and L-type Ca2+ current (I(Ca)). Western blot analysis revealed a reduced expression of Kv4.3 and MinK (to 25+/-21% and 48+/-15% of control, respectively) in diabetic dogs, while other channel proteins were unchanged (HERG, MiRP1, alpha(1c)) or increased (Kv1.4, KChIP2, KvLQT1). Insulin substitution fully prevented the diabetes-induced changes in I(Ks), KvLQT1 and MinK, however, the changes in I(to), Kv4.3, and Kv1.4 were only partially diminished by insulin., Conclusion: It is concluded that type 1 diabetes mellitus, although only moderately, lengthens ventricular repolarization, attenuates the repolarization reserve by decreasing I(to) and I(Ks) currents, and thereby may markedly enhance the risk of sudden cardiac death.
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- 2007
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45. Cardiomyopathies and sudden cardiac death caused by RyR2 mutations: are the channels the beginning and the end?
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Jóna I and Nánási PP
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- Genetic Predisposition to Disease, Humans, Cardiomyopathies genetics, Death, Sudden, Cardiac etiology, Mutation, Ryanodine Receptor Calcium Release Channel genetics
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- 2006
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46. Beta-adrenoceptor activation plays a role in the reverse rate-dependency of effective refractory period lengthening by dofetilide in the guinea-pig atrium, in vitro.
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Kovács A, Magyar J, Bányász T, Nánási PP, and Szénási G
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- Animals, Cardiac Pacing, Artificial, Dose-Response Relationship, Drug, Electric Stimulation, Guinea Pigs, Heart Atria drug effects, In Vitro Techniques, Male, Papillary Muscles drug effects, Papillary Muscles metabolism, Rabbits, Adrenergic beta-Antagonists pharmacology, Heart Atria metabolism, Myocardial Contraction drug effects, Phenethylamines pharmacology, Potassium Channel Blockers pharmacology, Receptors, Adrenergic, beta metabolism, Sulfonamides pharmacology
- Abstract
1. Blockers of the rapid component of the delayed rectifier potassium current (I(Kr)) prolong cardiac action potential duration (APD) and effective refractory period (ERP) in a reverse rate-dependent manner. Since activation of beta-adrenoceptors attenuates prolongation of APD evoked by I(Kr) blockers, rate-dependent neuronal noradrenaline liberation in the myocardium may contribute to the reverse rate-dependent nature of the effects of I(Kr) blockers. In order to test this hypothesis, we studied the effects of dofetilide, a pure I(Kr) blocker, on ERP after activation or blockade of beta-adrenoceptors and after catecholamine depletion in guinea-pig left atrial myocardium paced at 3, 2 and 1 Hz, in vitro. 2. Dofetilide (100 nM) lengthened ERP in a reverse rate-dependent manner in the left atrial myocardium of guinea-pigs. Strong activation of beta-adrenoceptors using 10 nM isoproterenol abolished the dofetilide-induced lengthening of ERP at all pacing rates. 3. Blockade of the beta-adrenoceptors with metoprolol (1 micro M), atenolol (3 micro M) or propranolol (300 nM) increased the dofetilide-evoked prolongation of ERP at 3 and 2 Hz, but not at 1 Hz. As a consequence, metoprolol attenuated while propranolol and atenolol fully eliminated the reverse rate-dependent nature of the dofetilide-induced ERP lengthening. In catecholamine-depleted atrial preparations of the guinea-pig (24 h pretreatment with 5 mg kg(-1) reserpine i.p.), the effect of dofetilide on ERP was not frequency dependent, and propranolol did not alter the effects of dofetilide. 4. In contrast to results obtained in guinea-pig atrial preparations, propranolol failed to change the reverse rate-dependent effect of dofetilide on ERP in the right ventricular papillary muscles of rabbits and guinea-pigs. 5. As an indication of the functional consequences of rate-dependent noradrenaline liberation, propranolol decreased twitch tension at 3 and 2 Hz but not at 1 Hz in the atrial myocardium of control guinea-pigs, whereas no such effect was detected in catecholamine-depleted atrial preparations. Propranolol failed to change contractility of ventricular myocardium in guinea-pigs and rabbits. 6. It is concluded that rate-dependent noradrenaline release and the ensuing beta-adrenoceptor activation contributed to the reverse rate-dependent nature of ERP prolongation caused by I(Kr) blockers in isolated guinea-pig atrial myocardium.
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- 2003
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47. Effect of thymol on kinetic properties of Ca and K currents in rat skeletal muscle.
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Szentandrássy N, Szentesi P, Magyar J, Nánási PP, and Csernoch L
- Subjects
- Animals, Culture Techniques, Dose-Response Relationship, Drug, Electric Conductivity, Female, Kinetics, Male, Muscle, Skeletal drug effects, Patch-Clamp Techniques, Rats, Antioxidants pharmacology, Calcium Channels metabolism, Muscle, Skeletal physiology, Potassium Channels metabolism, Thymol pharmacology
- Abstract
Background: Thymol is widely used as a general antiseptic and antioxidant compound in the medical practice and industry, and also as a stabilizer to several therapeutic agents, including halothane. Thus intoxication with thymol may occur in case of ingestion or improper anesthesia. In the present study, therefore, concentration-dependent effects of thymol (30-600 micro-grams) were studied on calcium and potassium currents in enzymatically isolated rat skeletal muscle fibers using the double vaseline gap voltage clamp technique., Results: Thymol suppressed both Ca and K currents in a concentration-dependent manner, the EC50 values were 193 +/- 26 and 93 +/- 11 microM, with Hill coefficients of 2.52 +/- 0.29 and 1.51 +/- 0.18, respectively. Thymol had a biphasic effect on Ca current kinetics: time to peak current and the time constant for inactivation increased at lower (100-200 microM) but decreased below their control values at higher (600 microM) concentrations. Inactivation of K current was also significantly accelerated by thymol (200-300 microM). These effects of thymol developed rapidly and were partially reversible. In spite of the marked effects on the time-dependent properties, thymol caused no change in the current-voltage relationship of Ca and K peak currents., Conclusions: Present results revealed marked suppression of Ca and K currents in skeletal muscle, similar to results obtained previously in cardiac cells. Furthermore, it is possible that part of the suppressive effects of halothane on Ca and K currents, observed experimentally, may be attributed to the concomitant presence of thymol in the superfusate.
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- 2003
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48. Endocardial versus epicardial differences in L-type calcium current in canine ventricular myocytes studied by action potential voltage clamp.
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Bányász T, Fülöp L, Magyar J, Szentandrássy N, Varró A, and Nánási PP
- Subjects
- Action Potentials drug effects, Action Potentials physiology, Animals, Calcium Channel Blockers pharmacology, Calcium Channels, L-Type drug effects, Dogs, Female, Male, Myocytes, Cardiac drug effects, Nisoldipine pharmacology, Patch-Clamp Techniques, Calcium Channels, L-Type physiology, Endocardium metabolism, Myocytes, Cardiac metabolism, Pericardium metabolism
- Abstract
Objectives: The aim of the present study was to assess and compare the dynamics of L-type Ca(2+) current (I(Ca,L)) during physiologic action potential (AP) in canine ventricular cardiomyocytes of epicardial (EPI) and endocardial (ENDO) origin., Methods: I(Ca,L) was recorded on cells derived from the two regions of the heart using both AP voltage clamp and conventional whole cell voltage clamp techniques., Results: AP voltage clamp experiments revealed that the decay of I(Ca,L) is monotonic during endocardial AP, whereas the current is double-peaked (displaying a second rise) during epicardial AP. The amplitude of the first peak was significantly greater in ENDO (-4.6+/-0.8 pA/pF) than in EPI cells (-2.8+/-0.3 pA/pF). Application of epicardial APs as command pulses to endocardial cells yielded double-peaked I(Ca,L) profiles, and increased the net charge entry carried by I(Ca,L) during the AP from 0.187+/-0.059 to 0.262+/-0.056 pC/pF (n=5, P<0.05). No differences were observed in current densities and inactivation kinetics of I(Ca,L) between EPI and ENDO cells when studied under conventional voltage clamp conditions. Nisoldipine shortened action potentials and eliminated the dome of the epicardial AP., Conclusion: I(Ca,L) was shown to partially inactivate before and deactivate during phase-1 repolarization and reopening of these channels is responsible for the formation of the dome in canine EPI cells. The transmural differences in the profile of I(Ca,L) could be well explained with differences in AP configuration.
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- 2003
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49. Differential effects of fluoxetine enantiomers in mammalian neural and cardiac tissues.
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Magyar J, Rusznák Z, Harasztosi C, Körtvély A, Pacher P, Bányász T, Pankucsi C, Kovács L, Szûcs G, Nánási PP, and Kecskeméti V
- Subjects
- Animals, Anticonvulsants pharmacology, Barium metabolism, Calcium metabolism, Dogs, Rats, Antidepressive Agents, Second-Generation pharmacology, Calcium Channels drug effects, Fluoxetine pharmacology, Myocytes, Cardiac drug effects, Neurons drug effects, Selective Serotonin Reuptake Inhibitors pharmacology
- Abstract
Racemic fluoxetine is a widely used SSRI antidepressant compound having also anticonvulsant effect. In addition, it was shown that it blocked several types of voltage gated ion channels including neural and cardiac calcium channels. In the present study the effects of enantiomers of fluoxetine (R(-)-fluoxetine and S(+)-fluoxetine) were compared on neuronal and cardiac voltage-gated Ca2+ channels using the whole cell configuration of patch clamp techniques, and the anticonvulsant action of these enantiomers was also evaluated in a mouse epilepsy model. In isolated pyramidal neurons of the dorsal cochlear nucleus of the rat the effect of fluoxetine (S(+), R(-) and racemic) was studied on the Ca2+ channels by measuring peak Ba2+ current during ramp depolarizations. All forms of fluoxetine reduced the Ba2+ current of the pyramidal cells in a concentration-dependent manner, with a Kd value of 22.3+/-3.6 microM for racemic fluoxetine. This value of Kd was higher by one order of magnitude than found in cardiac myocytes with fluoxetine enantiomers (2.4+/-0.1 and 2.8+/-0.2 microM). Difference between the effects of the two enantiomers on neuronal Ba2+ current was observed only at 5 microM concentration: R(-)-fluoxetine inhibited 28+/-3% of the peak current, while S(+)-fluoxetine reduced the current by 18+/-2% (n=13, P<0.05). In voltage clamped canine ventricular cardiomyocytes both enantiomers of fluoxetine caused a reversible concentration-dependent block of the peak Ca2+ current measured at 0 mV. Significant differences between the two enantiomers in this blocking effect was observed at low concentrations only: S(+)-fluoxetine caused a higher degree of block than R(-)-fluoxetine (56.3+/-2.2% versus 49.1+/-2.2% and 95.5+/-0.9% versus 84.5+/-3.1% block with 3 and 10 microM S(+) and R(-)-fluoxetine, respectively, P<0.05, n=5). Studied in current clamp mode, micromolar concentrations of fluoxetine shortened action potential duration of isolated ventricular cells, while higher concentrations also suppressed maximum velocity of depolarization and action potential amplitude. This shortening effect was significantly greater in the case of S(+) than R(-)-fluoxetine at 1 and 3 microM concentrations, whereas no differences in their effects on depolarization were observed. In pentylenetetrazole-induced mouse epilepsy model fluoxetine pretreatment significantly increased the 60 min survival rate, survival duration and seizure latency. These effects were more pronounced with the R(-) than the S(+) enantiomer. The results indicate that fluoxetine exerts much stronger suppressive effect on cardiac than neuronal calcium channels. At micromolar concentrations (between 1 and 10 microM) R(-)-fluoxetine is more effective than the S(+) enantiomer on neuronal, while less effective on cardiac calcium channels. The stronger anticonvulsant effect of the R(-) enantiomer may, at least partially, be explained by these differences. Used as an antidepressant or anticonvulsant drug, less severe cardiac side-effects are anticipated with the R(-) enantiomer.
- Published
- 2003
50. Effects of thymol on calcium and potassium currents in canine and human ventricular cardiomyocytes.
- Author
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Magyar J, Szentandrássy N, Bányász T, Fülöp L, Varró A, and Nánási PP
- Subjects
- Action Potentials drug effects, Action Potentials physiology, Animals, Dogs, Dose-Response Relationship, Drug, Heart Ventricles cytology, Heart Ventricles drug effects, Humans, Ventricular Function, Calcium Channels physiology, Myocardium cytology, Myocardium metabolism, Potassium Channels physiology, Thymol pharmacology
- Abstract
1. Concentration-dependent effects of thymol (1 - 1000 microM) was studied on action potential configuration and ionic currents in isolated canine ventricular cardiomyocytes using conventional microelectrode and patch clamp techniques. 2. Low concentration of thymol (10 microM) removed the notch of the action potential, whereas high concentrations (100 microM or higher) caused an additional shortening of action potential duration accompanied by progressive depression of plateau and reduction of V(max). 3. In the canine cells L-type Ca current (I(Ca)) was decreased by thymol in a concentration-dependent manner (EC(50): 158+/-7 microM, Hill coeff.: 2.96+/-0.43). In addition, thymol (50 - 250 microM) accelerated the inactivation of I(Ca), increased the time constant of recovery from inactivation, shifted the steady-state inactivation curve of I(Ca) leftwards, but voltage dependence of activation remained unaltered. Qualitatively similar results were obtained with thymol in ventricular myocytes isolated from healthy human hearts. 4. Thymol displayed concentration-dependent suppressive effects on potassium currents: the transient outward current, I(to) (EC(50): 60.6+/-11.4 microM, Hill coeff.: 1.03+/-0.11), the rapid component of the delayed rectifier, I(Kr) (EC(50): 63.4+/-6.1 microM, Hill coeff.: 1.29+/-0.15), and the slow component of the delayed rectifier, I(Ks) (EC(50): 202+/-11 microM, Hill coeff.: 0.72+/-0.14), however, K channel kinetics were not much altered by thymol. These effects on Ca and K currents developed rapidly (within 0.5 min) and were readily reversible. 5. In conclusion, thymol suppressed cardiac ionic channels in a concentration-dependent manner, however, both drug-sensitivities as well as the mechanism of action seems to be different when blocking calcium and potassium channels.
- Published
- 2002
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