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Your search keyword '"Neolyngbya"' showing total 4 results
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4 results on '"Neolyngbya"'

1. Metabolomic Characterization of a cf. Neolyngbya Cyanobacterium from the South China Sea Reveals Wenchangamide A, a Lipopeptide with In Vitro Apoptotic Potential in Colon Cancer Cells

Author

Lijian Ding, Rinat Bar-Shalom, Dikla Aharonovich, Naoaki Kurisawa, Gaurav Patial, Shuang Li, Shan He, Xiaojun Yan, Arihiro Iwasaki, Kiyotake Suenaga, Chengcong Zhu, Haixi Luo, Fuli Tian, Fuad Fares, C. Benjamin Naman, and Tal Luzzatto-Knaan

Subjects
metabolomics, secondary metabolites, natural products, cyanobacteria, Neolyngbya, anticancer, Biology (General), QH301-705.5
Abstract

Metabolomics can be used to study complex mixtures of natural products, or secondary metabolites, for many different purposes. One productive application of metabolomics that has emerged in recent years is the guiding direction for isolating molecules with structural novelty through analysis of untargeted LC-MS/MS data. The metabolomics-driven investigation and bioassay-guided fractionation of a biomass assemblage from the South China Sea dominated by a marine filamentous cyanobacteria, cf. Neolyngbya sp., has led to the discovery of a natural product in this study, wenchangamide A (1). Wenchangamide A was found to concentration-dependently cause fast-onset apoptosis in HCT116 human colon cancer cells in vitro (24 h IC50 = 38 μM). Untargeted metabolomics, by way of MS/MS molecular networking, was used further to generate a structural proposal for a new natural product analogue of 1, here coined wenchangamide B, which was present in the organic extract and bioactive sub-fractions of the biomass examined. The wenchangamides are of interest for anticancer drug discovery, and the characterization of these molecules will facilitate the future discovery of related natural products and development of synthetic analogues.

Published
2021
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2. Metabolomic Characterization of a cf. Neolyngbya Cyanobacterium from the South China Sea Reveals Wenchangamide A, a Lipopeptide with In Vitro Apoptotic Potential in Colon Cancer Cells

Author

Shuang Li, Shan He, C. Benjamin Naman, Chengcong Zhu, Lijian Ding, Fuad Fares, Naoaki Kurisawa, Haixi Luo, Arihiro Iwasaki, Kiyotake Suenaga, Fuli Tian, Rinat Bar-Shalom, Xiaojun Yan, Dikla Aharonovich, Tal Luzzatto-Knaan, and Gaurav Patial

Subjects
0301 basic medicine, Cyanobacteria, Aquatic Organisms, China, wenchangamide, Colorectal cancer, natural products, QH301-705.5, Pharmaceutical Science, South China Sea, anticancer, 01 natural sciences, cyanobacteria, Article, drug discovery, 03 medical and health sciences, chemistry.chemical_compound, Lipopeptides, Metabolomics, Cell Line, Tumor, medicine, Animals, Humans, Neolyngbya, Biology (General), Pharmacology, Toxicology and Pharmaceutics (miscellaneous), Cell Proliferation, Biological Products, Natural product, biology, 010405 organic chemistry, Drug discovery, secondary metabolites, Lipopeptide, biology.organism_classification, medicine.disease, metabolomics, In vitro, 0104 chemical sciences, 030104 developmental biology, chemistry, Biochemistry, Apoptosis
Abstract

Metabolomics can be used to study complex mixtures of natural products, or secondary metabolites, for many different purposes. One productive application of metabolomics that has emerged in recent years is the guiding direction for isolating molecules with structural novelty through analysis of untargeted LC-MS/MS data. The metabolomics-driven investigation and bioassay-guided fractionation of a biomass assemblage from the South China Sea dominated by a marine filamentous cyanobacteria, cf. Neolyngbya sp., has led to the discovery of a natural product in this study, wenchangamide A (1). Wenchangamide A was found to concentration-dependently cause fast-onset apoptosis in HCT116 human colon cancer cells in vitro (24 h IC50 = 38 μM). Untargeted metabolomics, by way of MS/MS molecular networking, was used further to generate a structural proposal for a new natural product analogue of 1, here coined wenchangamide B, which was present in the organic extract and bioactive sub-fractions of the biomass examined. The wenchangamides are of interest for anticancer drug discovery, and the characterization of these molecules will facilitate the future discovery of related natural products and development of synthetic analogues.

Published
2021

3. Isolation and Characterization of Potential Neurotoxic Cyanobacterial Metabolites Associated with Intoxication of Captive Bottlenose Dolphins in the Florida Keys

Author

Lydon, Christina and Lydon, Christina

Abstract

Bottlenose dolphins (Tursiops truncatus) living in captivity in the Florida Keys have recently been observed grazing macrophytic algal communities, and particularly, filamentous cyanobacteria, within their enclosures, and subsequently presenting apparent signs of intoxication. Collections of a mixed assemblage of marine filamentous cyanobacteria were morphologically related to the taxonomically complex and toxigenic polyphyletic genus, Lyngbya, sensu lato. Phylogenetic characterization, using 16S rDNA methods, identified an undescribed member of the recently accepted genus Neolyngbya (Oscillatoriales) among Neolyngbya arenicola, and an unknown Oscillatoriacean species within a poorly resolved clade clustering between the genera Limnoraphis and Capilliphycus. A dual approach was subsequently utilized to identify a putative neurotoxic metabolite from cyanobacteria associated with the intoxication events. Initial chemical screening was unable to detect recognized algal and cyanobacterial neurotoxins including anatoxin-a, brevetoxin-2 (PbTx-2), domoic acid, b-methylamino-L-alanine and saxitoxin. In tandem, however, toxicity testing – and concomitant bioassay-guided fractionation- based on early life stages (i.e., embryo, larvae) of the zebrafish (Danio rerio) as a vertebrate toxicological model, was employed to identify relevant metabolites from cyanobacterial collections. Using the latter approach, relevant toxicity including, particularly, endpoints of neurotoxicity were identified for extracts and subsequent fractions. Apparent neurotoxicity was comparatively assessed against the recognized algal neurotoxin, PbTx-2. Crude lipophilic extracts, and subsequent chemical fractions, notably aligned with observed neurotoxic effects of PbTx-2. Further bioassay-guided fractionation enabled purification and structural elucidation of a previously undocumented, neurotoxic metabolite, (4S,5R,6R,7S,10S)-eudesman-(4S,6R)-cyclocarbonate (i.e., eudesmacarbonate), from the mixed filam

Published
2020

4. Metabolomic Characterization of a cf. Neolyngbya Cyanobacterium from the South China Sea Reveals Wenchangamide A, a Lipopeptide with In Vitro Apoptotic Potential in Colon Cancer Cells.

Author

Ding, Lijian, Bar-Shalom, Rinat, Aharonovich, Dikla, Kurisawa, Naoaki, Patial, Gaurav, Li, Shuang, He, Shan, Yan, Xiaojun, Iwasaki, Arihiro, Suenaga, Kiyotake, Zhu, Chengcong, Luo, Haixi, Tian, Fuli, Fares, Fuad, Naman, C. Benjamin, and Luzzatto-Knaan, Tal

Abstract

Metabolomics can be used to study complex mixtures of natural products, or secondary metabolites, for many different purposes. One productive application of metabolomics that has emerged in recent years is the guiding direction for isolating molecules with structural novelty through analysis of untargeted LC-MS/MS data. The metabolomics-driven investigation and bioassay-guided fractionation of a biomass assemblage from the South China Sea dominated by a marine filamentous cyanobacteria, cf. Neolyngbya sp., has led to the discovery of a natural product in this study, wenchangamide A (1). Wenchangamide A was found to concentration-dependently cause fast-onset apoptosis in HCT116 human colon cancer cells in vitro (24 h IC50 = 38 μM). Untargeted metabolomics, by way of MS/MS molecular networking, was used further to generate a structural proposal for a new natural product analogue of 1, here coined wenchangamide B, which was present in the organic extract and bioactive sub-fractions of the biomass examined. The wenchangamides are of interest for anticancer drug discovery, and the characterization of these molecules will facilitate the future discovery of related natural products and development of synthetic analogues. [ABSTRACT FROM AUTHOR]

Published
2021
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