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(1) Background: Wounds with damages to the subcutaneous are difficult to regenerate because of the tissue damages and complications such as bacterial infection. (2) Methods: In this study, we created burn wounds on pigs and investigated the efficacy of three biomaterials: polycaprolactone‐gelatin‐silver membrane (PCLGelAg) and two commercial burn dressings, Aquacel® Ag and UrgoTul™ silver sulfadiazine. In vitro long‐term antibacterial property and in vivo wound healing performance were investigated. Agar diffusion assays were employed to evaluate bacterial inhibition at different time intervals. Minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC) and time‐kill assays were used to compare antibacterial strength among samples. Second‐degree burn wounds in the pig model were designed to evaluate the efficiency of all dressings in supporting the wound healing process. (3) Results: The results showed that PCLGelAg membrane was the most effective in killing both Gram‐positive and Gram-negative bacteria bacteria with the lowest MBC value. All three dressings (PCLGelAg, Aquacel, and UrgoTul) exhibited bactericidal effect during the first 24 h, supported wound healing as well as prevented infection and inflammation. (4) Conclusions: The results suggest that the PCLGelAg membrane is a practical solution for the treatment of severe burn injury and other infection‐related skin complications.

An injectable bone substitute (IBS) with well-controlled release mechanism of simvastatin (SIM) was fabricated from SIM loaded-porous poly(lactic-co-glycolic acid) microspheres (PLGA MSs), biphasic calcium phosphates (BCP) and CaCO3 nano powder as the solid phase and gelatin, citric acid and hydroxypropyl methylcellulose as the liquid phase. The ratios of PLGA MSs and BCP were varied to obtain IBS with optimal mechanical properties, biocompatibility and bioactivity. In vitro test with MG-63 pre-osteoblast cells showed that IBS with higher amount of PLGA MSs yield better cell adhesion and proliferation. In vivo study on rabbit femoral defect also suggested that the IBS promoted osteogenesis.

This study aims to investigate the effect of chitosan and gelatin as a stabilizer of silver nanoparticles (Ag NPs) and on their application designs. Ag NPs hydrogels was synthesized and stabilized by capping agents (chitosan and gelatin). Then, Ag NPs membrane was prepared by coating Ag NPs hydrogel on PCL fibrils. Optical and microscopic observation as well as structural properties were studied to compare two stabilizers. Antimicrobial testing was done on both Ag NPs hydrogels and membranes. The results suggest that gelatin provides better stabilizing activity and superior antimicrobial potency in membrane form while chitosan exhibits better antibacterial inhibition in hydrogel form.