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1. Reversibly immortalized keratinocytes (iKera) facilitate re-epithelization and skin wound healing: Potential applications in cell-based skin tissue engineering

Author

Jiamin Zhong, Hao Wang, Ke Yang, Huifeng Wang, Chongwen Duan, Na Ni, Liqin An, Yetao Luo, Piao Zhao, Yannian Gou, Shiyan Sheng, Deyao Shi, Connie Chen, William Wagstaff, Bryce Hendren-Santiago, Rex C. Haydon, Hue H. Luu, Russell R. Reid, Sherwin H. Ho, Guillermo A. Ameer, Le Shen, Tong-Chuan He, and Jiaming Fan

Subjects
Keratinocytes, Skin tissue engineering, Reversible immortalization, SV40 large T antigen, PPCN, Skin wound healing, Materials of engineering and construction. Mechanics of materials, TA401-492, Biology (General), QH301-705.5
Abstract

Skin injury is repaired through a multi-phase wound healing process of tissue granulation and re-epithelialization. Any failure in the healing process may lead to chronic non-healing wounds or abnormal scar formation. Although significant progress has been made in developing novel scaffolds and/or cell-based therapeutic strategies to promote wound healing, effective management of large chronic skin wounds remains a clinical challenge. Keratinocytes are critical to re-epithelialization and wound healing. Here, we investigated whether exogenous keratinocytes, in combination with a citrate-based scaffold, enhanced skin wound healing. We first established reversibly immortalized mouse keratinocytes (iKera), and confirmed that the iKera cells expressed keratinocyte markers, and were responsive to UVB treatment, and were non-tumorigenic. In a proof-of-principle experiment, we demonstrated that iKera cells embedded in citrate-based scaffold PPCN provided more effective re-epithelialization and cutaneous wound healing than that of either PPCN or iKera cells alone, in a mouse skin wound model. Thus, these results demonstrate that iKera cells may serve as a valuable skin epithelial source when, combining with appropriate biocompatible scaffolds, to investigate cutaneous wound healing and skin regeneration.

Published
2022
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2. Reversibly immortalized keratinocytes (iKera) facilitate re-epithelization and skin wound healing: Potential applications in cell-based skin tissue engineering

Author

Liqin An, Hao Wang, Le Shen, Bryce Hendren-Santiago, William Wagstaff, Shiyan Sheng, Yannian Gou, Huifeng Wang, Russell R. Reid, Jiaming Fan, Na Ni, Piao Zhao, Deyao Shi, Sherwin H. Ho, Connie Chen, Chongwen Duan, Hue H. Luu, Guillermo A. Ameer, Rex C. Haydon, Ke Yang, Yetao Luo, Tong-Chuan He, and Jiamin Zhong

Subjects
Keratinocytes, Scaffold, Skin wound, PPCN, QH301-705.5, Cell, Biomedical Engineering, Article, Biomaterials, Skin tissue, medicine, Skin wound healing, Reversible immortalization, Biology (General), Materials of engineering and construction. Mechanics of materials, Skin tissue engineering, integumentary system, business.industry, Regeneration (biology), medicine.anatomical_structure, Cancer research, TA401-492, SV40 large T antigen, Keratinocyte, Wound healing, business, Re epithelization, Biotechnology
Abstract

Skin injury is repaired through a multi-phase wound healing process of tissue granulation and re-epithelialization. Any failure in the healing process may lead to chronic non-healing wounds or abnormal scar formation. Although significant progress has been made in developing novel scaffolds and/or cell-based therapeutic strategies to promote wound healing, effective management of large chronic skin wounds remains a clinical challenge. Keratinocytes are critical to re-epithelialization and wound healing. Here, we investigated whether exogenous keratinocytes, in combination with a citrate-based scaffold, enhanced skin wound healing. We first established reversibly immortalized mouse keratinocytes (iKera), and confirmed that the iKera cells expressed keratinocyte markers, and were responsive to UVB treatment, and were non-tumorigenic. In a proof-of-principle experiment, we demonstrated that iKera cells embedded in citrate-based scaffold PPCN provided more effective re-epithelialization and cutaneous wound healing than that of either PPCN or iKera cells alone, in a mouse skin wound model. Thus, these results demonstrate that iKera cells may serve as a valuable skin epithelial source when, combining with appropriate biocompatible scaffolds, to investigate cutaneous wound healing and skin regeneration., Graphical abstract Image 1, Highlights • A reversibly immortalized mouse keratinocyte (i.e., iKera) line was established. • The iKera cells maintain the characteristics of keratinocytes without tumorigenecity. • A combination of thermoresponsive scaffold PPCN and iKera cells facilitates re-epithelization and cutaneous wound healing. • The iKera cells are a valuable cell source for skin tissue engineering studies.

Published
2022

3. Impact de différents supports respiratoires nasaux sur la sécurité de l’alimentation orale chez l’agneau nouveau-né

Author

Alain, Charles, Praud, Jean-Paul, Alain, Charles, and Praud, Jean-Paul

Abstract

Contexte : Les nourrissons avec bronchiolite virale aiguë sévère requièrent fréquemment un support respiratoire nasal (SRN) tel une pression positive continue nasale (PPCn) ou des lunettes nasales à haut débit (LNHD). L'alimentation orale de ces nourrissons sous SRN demeure controversée par crainte d'aspirations menant à des événements cardiorespiratoires, tels que des apnées et/ou bradycardies et/ou désaturations. L’objectif principal de cette étude était d’évaluer la sécurité de l’alimentation orale sous différents SRN chez un modèle ovin de nouveau-né à terme. Méthodes : huit agneaux âgés de 4 à 5 jours ont été étudiés. Une instrumentation chirurgicale était réalisée sous sédation consciente et anesthésie locale afin d’enregistrer la succion, la déglutition, la respiration, l'ECG, la saturation et les gaz artériels (cathéter carotidien). Chaque agneau prenait sous vidéofluoroscopie 60 mL de lait, soit 2 biberons de 30 mL séparés par une pause de 30 secondes, dans quatre conditions randomisées [PPCn 6 cmH 2 0, LNHD 7 L/min, LNHD PPCn et contrôle (sans SRN)]. L’étude se déroulait sur deux journées randomisées, avec et sans tachypnée standardisée induite par compression thoracique (brassard à tension). Des modèles linéaires généralisés multivariés ont permis de comparer les 4 SRN sur le plan de la sécurité (événements cardiorespiratoires et aspirations), la coordination succion-déglutition-respiration et l’efficacité de l’alimentation orale. Résultats : Des pénétrations laryngées et/ou aspirations trachéales sont survenus au cours de 6 séances d’alimentations au biberon parmi les 101 enregistrées sous vidéofluoroscopie. Bien qu’aucune différence statistiquement significative n’ait été retrouvée entre les SRN, 97% (104/107) des pénétrations laryngées ont été observées en condition LNHD ou LNHD PPCn . La condition LNHD a significativement altéré la coordination succion-déglutition-respiration sans toutefois compromettre la sécurité de l’alimentation orale. Conclusion

Published
2021

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