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Exosomal lncRNAs secreted by cancer cells can serve as potential biomarkers in the diagnosis and prognosis of various tumours. Here, we are committed to explore the diagnostic and prognostic value of serum exosomal XIST secreted by tumour cells to predict recurrence in patients with triple‐negative breast cancer (TNBC). Significant increments in XIST and exo‐XIST from tumour tissues and blood serum were found in reoccurring TNBC patients by comparison with non‐recurrences. Levels of serum exo‐XIST were only significantly increased in TNBC recurrence and no association with other clinicopathological parameters. Additionally, serum exo‐XIST levels could be served as an assessment of change in the load of triple‐negative breast cancer. Expressions of exo‐XIST were markedly decreased after resection of the primary breast tumours and obviously elevated at the time of recurrence. Finally, an obvious association was identified between serum exo‐XIST levels and a poorer overall survival (OS) in TNBC patients. Levels of serum exo‐XIST may serve as a diagnostic and prognostic biomarker to predict the recurrent TNBC‐loading status.

With the wide application of compost in agriculture, heavy metals (HMs) continue to accumulate in the soil environment, which poses a great threat to the health of the soil environment. Therefore, it is critical for effectively reduce the mobility of HMs. In this study, the influence of activated carbon (AC) addition time on mobility of HMs (Cu, Zn and Pb) and HMs resistant bacteria structure were evaluated during chicken manure composting. The result showed that the addition of AC in the thermophilic period could effectively reduce the mobility of HMs. Subsequently, high-throughput sequencing results showed that the dominant phyla were Proteobacteria, Firmicutes, Actinbacteria, Deinococcus-Thermus, Chloroflexi, Gemmatimonadetes and Bacteroidetes within the sample, which were ubiquitous and abundant in composting. The Redundancy analysis (RDA) results indicated that the mobility of HMs (Cu, Zn and Pb) by superior bacteria fate varied in AC amendment composting. Ultimately, a regulation method is proposed to influence the mobility of HMs by regulating the bacteria community in the AC compost. Our current studies suggest that the addition of AC during compost preparation (thermophilic period) is an effective strategy in regulating the mobility (bioavailability) of HMs, thereby significantly reducing environmental pollution problems.

The natural carotenoid lycopene (LYC) has strong antioxidant and neuroprotective capacities. This study investigated the effects and mechanisms of LYC on chronic stress-induced hippocampal lesions and learning and memory dysfunction. Rats were administered LYC and/or chronic restraint stress (CRS) for 21 days. Morris water maze results demonstrated that LYC prevented CRS-induced learning and memory dysfunction. Histopathological staining and transmission electron microscopy observations revealed that LYC ameliorated CRS-induced hippocampal microstructural and ultrastructural damage. Furthermore, LYC alleviated CRS-induced oxidative stress by reducing reactive oxygen species (ROS) production and enhancing antioxidant enzyme activities. LYC also improved CRS-induced hippocampal mitochondrial dysfunction by recovering mitochondrial membrane potential, and complex I (NADH dehydrogenase) and II (succinate dehydrogenase) activities. Moreover, LYC reduced CRS-induced apoptosis via the mitochondrial apoptotic pathway, and decreased the number of terminal deoxynucleotidyl transferase dUTP nick-end-labeled positive cells. Additionally, western blot analysis demonstrated that LYC inhibited CRS-induced activation of the c-Jun N-terminal kinase (JNK) signaling pathway. Correlation analysis indicated that ROS levels, JNK activation, and the mitochondrial apoptotic pathway were positively correlated. Further investigation of the underlying mechanisms revealed that the ROS scavenger N-acetyl-l-cysteine inhibited CRS-induced JNK activation. Furthermore, the JNK inhibitor SP600125 relieved CRS-induced hippocampal mitochondrial dysfunction, apoptosis via the mitochondrial apoptotic pathway, and learning and memory dysfunction. Together, these results suggest that LYC alleviates hippocampal oxidative stress, mitochondrial dysfunction, and apoptosis by inhibiting the ROS/JNK signaling pathway, thereby improving CRS-induced hippocampal injury and learning and memory dysfunction. This study provides a theoretical basis and new therapeutic strategies for the application of LYC to relieve chronic stress encephalopathy.

Ceramic thermal barrier coatings (TBCs) are widely used on the gas turbine engine. As one of the candidate TBC materials, LaPO4 has attracted much attention. In the present study, the bulk LaPO4 was prepared by spark plasma sintering (SPS). The effect of different preparation conditions on the properties of the obtained LaPO4 ceramic was studied. The phase composition, microstructure, thermal expansion and thermal conductivity of the LaPO4 ceramic with heat treatment at 1450°C for different time were studied by XRD and SEM.

China has long history in exploiting, processing and using of NORM/TENORM materials and has also paid attention to control and use properly of these materials. Although radiation level in these industry fields has been investigated and primary regulatory principles have been established, some problems still exist such as having not practicable criteria on exemption and clearance of residues and wastes. Considering the fact that NORM/TENORM is involved in a variety of industrial fields in China as well as its specific characteristics of large waste volume, low radioactivity concentration and long lived radionuclides contained in wastes. Regulatory polices should be considered carefully so that the contradiction between cost and benefit can be in balance.