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In this article, a Ka-band satellite communication (SATCOM) transceiver is first presented using a standard CMOS technology. The proposed Ka-band SATCOM transceiver consists of a high-linearity transmitter (TX) and dual-channel receiver (RX); both TX and RX are based on direct-conversion architecture. By implementing the dual-channel RX, multiple multiplexing modes, including polarization multiplexing and frequency multiplexing, can be enabled depending on the application. The RX variable gain is distributed in both RF blocks and baseband blocks to achieve a wide input dynamic range. The LNA employs a dual-coupling transformer for a low-noise figure (NF) and wideband input matching; moreover, it enhances the amplifier unilateralization characteristic to mitigate the loading effect from switched attenuators. An adjacent channel interference (ACI) cancellation scheme is proposed to further enhance the RX linearity in the frequency multiplexing mode. In the TX, single-turn high-quality-factor transformer is employed to realize matching network and four-way power combining. A prototype of the SATCOM transceiver is fabricated in a standard 65-nm CMOS process. Under 1.05-V supply voltage, the TX achieves a ${P{_{SAT}}}$ of 20.5 dBm and an average output power of 12 dBm with 2% error vector magnitude (EVM) and a 37.6-dB ACPR. The dual-channel RX achieves an NF of 5.4 dB, an IIP3 of -30 dBm on the high-gain mode, the ACI cancellation is measured of 7.9 dB with a 100-MHz signal bandwidth.

In this article, a mixed-signal, 32-kHz reference-based 2.4-GHz fractional-N over-sampling phase-locked loop (OSPLL) is proposed. Different from the conventional 1x sampling PLL, which only uses zero-crossing timing information of the reference signal, the proposed OSPLL fully utilizes both the voltage and timing domain information of the reference signal and realizes oversampling ratio (OSR) times phase detection (PD) in one reference cycle. The proposed OSPLL employs the digital-to-analog converter (DAC) to construct the reference-like feedback signal in the voltage domain and utilizes the digital-to-time converter (DTC) to improve PD resolution in the time domain. The adaptive lookup table (LuT)-based calibration is proposed to generate the correct information for DAC and DTC control. A clocked passive comparator works as a bang-bang phase detector (BBPD) for the PLL control and LuTs' construction. The co-design of low-noise analog circuits and digital calibrations enables good jitter and spur performance. The proposed OSPLL is fabricated in 65-nm CMOS technology, with the core area of 0.58 mm², and the power consumption is 4.97 mW with a 1-V power supply. It achieves 5.79-ps root-mean-square (rms) jitter in fractional-N modes with the loop-bandwidth (BW $_{loop}$ ) of 200 kHz, corresponding to the figures of merit (FoMs) of -217.8 dB. The measured fractional spur is less than -36 dBc, and the reference spur is -78 dBc, respectively.

Antisense long noncoding RNAs serve as important regulators of protein-coding genes and contribute to tumorigenesis and metastasis. AGAP2-AS1, an antisense lncRNA transcribed from AGAP2, is involved in various cancer types. However, the clinical significance, biological roles and regulatory mechanisms of AGAP2-AS1 in epithelial ovarian cancer (EOC) have not been thoroughly elucidated to date. In this study, we demonstrated the expression pattern and biological roles of AGAP2-AS1 in EOC. Clinically, AGAP2-AS1 expression was decreased in EOC tissues compared to that in the controls. Low expression of AGAP2-AS1 was associated with advanced FIGO stage, high histological grade, serous subtype and lymph node metastasis in patients with EOC. AGAP2-AS1 inhibited cell migration, invasion and proliferation in vitro. AGAP2-AS1 suppressed tumor growth in vivo. Mechanistically, AGAP2-AS1 inhibited cell metastasis and proliferation by downregulating KRAS, FGFR4, and CTSK and suppressing epithelial-mesenchymal transition. In conclusion, we provide the first evidence for the tumor-suppressing effect of AGAP2-AS1 in EOC and demonstrate that AGAP2-AS1 may represent a promising therapeutic target for EOC patients.

Additional file 4: Fig. S3. Two-factor model performance comparison

Additional file 3. Fig. S2. Three-factor model performance comparison

Additional file 16. Supplementary materials and methods.

Additional file 1: Table S1. Baseline characteristics of 345 patients with cervical adenocarcinoma. Table S2. The identification of cut-off value for intermediate risk factors in AC patients. Table S3. Performance comparison in best model. Table S4. Three-factor model performance comparison. Table S5. Three-factor model performance comparison

Xiaoyan Tang,1–3,* Songping Liu,4,* Yinglei Liu,5,* Xiaojing Lin,1–3 Tingting Zheng,1–3 Xin Liu,1–3 Junjun Qiu,1–3 Keqin Hua1–3 1Department of Gynecology, Obstetrics and Gynecology Hospital, Fudan University, Shanghai 200011, People’s Republic of China; 2Department of Obstetrics and Gynecology of Shanghai Medical College, Fudan University, Shanghai 200032, People’s Republic of China; 3Shanghai Key Laboratory of Female Reproductive Endocrine-Related Diseases, Obstetrics and Gynecology Hospital, Fudan University, Shanghai 200011, People’s Republic of China; 4Department of Obstetrics and Gynecology, Zhenjiang Maternal and Child Health Hospital, Zhenjiang, Jiangsu 212001, People’s Republic of China; 5Department of Obstetrics and Gynecology, Nantong First People’s Hospital, Nantong, Jiangsu 226001, People’s Republic of China*These authors contributed equally to this workCorrespondence: Junjun Qiu; Keqin HuaDepartment of Gynecology, Obstetrics and Gynecology Hospital of Fudan University, 419 Fangxie Road, Shanghai 200011, People’s Republic of ChinaTel +86 216 345 5050 ext 8261Fax +86 216 345 5090Email qiujunjun1113@163.com; huakeqin@fudan.edu.cnPurpose: Exosomes are key mediators of cellular communication by transporting molecules, including long noncoding RNAs (lncRNAs), and have been regarded as promising non-invasive biomarkers. This study aimed to evaluate the expression pattern and clinical significance of serum exosomal lncRNA antisense hypoxia inducible factor (aHIF) in epithelial ovarian cancer (EOC).Patients and methods: Sixty-two EOC patients in Obstetrics and Gynecology Hospital of Fudan University were enrolled. The expression levels of aHIF in tissues and serum exosomes were examined by RT-qPCR. The origin of serum exosomal aHIF was explored in vitro and in vivo. Univariate and multivariate Cox regression analyses were used to evaluate the prognostic factors of EOC. A prognostic predictive nomogram was formulated in R software.Results: We isolated exosomes, identified exosomal aHIF in the serum of EOC patients. The expression of serum exosomal aHIF was higher in EOC patients and was correlated with the aHIF level in EOC tissues. In vitro and in vivo, the results indicated that serum exosomal aHIF was derived from tumor cells. Kaplan-Meier survival analysis demonstrated that EOC patients with higher serum exosomal aHIF expression had poorer overall survival. Cox multivariate regression model revealed that FIGO stage, residual tumor size, and serum exosomal aHIF level were independent prognostic factors of EOC. Based on the prognostic value of serum exosomal aHIF, we established a nomogram model that showed a good predictive ability for EOC patients.Conclusion: Serum exosomal aHIF is overexpressed in EOC and can serve as a noninvasive predictive biomarker for unfavorable prognosis.Keywords: epithelial ovarian cancer;EOC, exosome, antisense hypoxia inducible factor, aHIF, nomogram, biomarkerCorrigendum for this paper has been published

Xiaoyan Tang,1– 3,* Songping Liu,4,* Yan Ding,1– 3,* Chenyan Guo,1– 3 Jingjing Guo,1– 3 Keqin Hua,1– 3 Junjun Qiu1– 3 1Department of Gynecology, Obstetrics and Gynecology Hospital, Fudan University, Shanghai 200011, People’s Republic of China; 2Department of Obstetrics and Gynecology of Shanghai Medical College, Fudan University, Shanghai 200032, People’s Republic of China; 3Shanghai Key Laboratory of Female Reproductive Endocrine-Related Diseases, Shanghai 200011, People’s Republic of China; 4Department of Obstetrics and Gynecology, Zhenjiang Maternal and Child Health Hospital, Zhenjiang, Jiangsu 212001, People’s Republic of China*These authors contributed equally to this workCorrespondence: Junjun Qiu; Keqin HuaDepartment of Gynecology, Obstetrics and Gynecology Hospital of Fudan University, 419 Fangxie Road, Shanghai 200011, People’s Republic of ChinaTel +86-21-63455050 Ext 8261Fax +86-21-63455090Email qiujunjun1113@163.com; huakeqin@fudan.edu.cnPurpose: Circular RNAs (circRNAs) are novel type of noncoding RNAs that play important roles and serve as noninvasive biomarkers in various cancers. In the present study, we focused on circFoxO3a and aimed to investigate its prognostic value as a novel serum biomarker for squamous cervical cancer (SCC).Patients and Methods: Our study included 103 SCC patients from Obstetrics and Gynecology Hospital of Fudan University. Expression levels of circFoxO3a in the serum of patients with SCC were examined by reverse transcription‑quantitative PCR (RT‑qPCR). The correlation between serum circFoxO3a expression and clinicopathologic factors was analyzed. The Kaplan–Meier method and multivariate Cox regression analysis were applied to evaluate the independent prognostic factors for SCC. A prognostic predictive nomogram was constructed using R software.Results: Levels of serum circFoxO3a were decreased in SCC patients compared with controls. Low expression of circFoxO3a was correlated with deeper stromal invasion and positive lymph node metastasis. Moreover, SCC patients with lower expression of serum circFoxO3a showed poorer prognosis, including both overall survival (OS) and recurrence-free survival (RFS). Multivariate Cox analysis indicated low serum circFoxO3a levels to be an unfavorable prognostic factor for both OS and RFS, independent of positive lymph node metastasis. Notably, the predictive nomogram we established further confirmed that serum circFoxO3a is a useful tool for predicting survival in SCC.Conclusion: Altogether, our findings demonstrated that serum circFoxO3a could serve as a potential novel noninvasive predictive prognostic biomarker and therapeutic target for SCC.Keywords: circular RNA, FoxO3a, squamous cervical cancer, biomarker &nbsp