1. Statistical Optimisation of Streptomyces sp. DZ 06 Keratinase Production by Submerged Fermentation of Chicken Feather Meal
- Author
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Samir Hamma, Nawel Boucherba, Zahra Azzouz, Marilize Le Roes-Hill, Ourdia-Nouara Kernou, Azzeddine Bettache, Rachid Ladjouzi, Rima Maibeche, Mohammed Benhoula, Hakim Hebal, Zahir Amghar, Narimane Allaoua, Kenza Moussi, Patricia Rijo, and Said Benallaoua
- Subjects
keratin residues ,chicken feather meal ,keratinase activity ,actinobacteria ,biodegradation ,Plackett–Burman ,Fermentation industries. Beverages. Alcohol ,TP500-660 - Abstract
This study focused on the isolation of actinobacteria capable of producing extracellular keratinase from keratin-rich residues, which led to the selection of an actinobacterial strain referenced as Streptomyces strain DZ 06 (ES41). The Plackett–Burman screening plan was used for the statistical optimization of the enzymatic production medium, leading to the identification of five key parameters that achieved a maximum activity of 180.1 U/mL. Further refinement using response surface methodology (RSM) with a Box–Behnken design enhanced enzyme production to approximately 458 U/mL. Model validation, based on the statistical predictions, demonstrated that optimal keratinase activity of 489.24 U/mL could be attained with 6.13 g/L of chicken feather meal, a pH of 6.25, incubation at 40.65 °C for 4.11 days, and an inoculum size of 3.98 × 107 spores/mL. The optimized culture conditions yielded a 21.67-fold increase in keratinase compared with the initial non-optimized standard conditions. The results show that this bacterium is an excellent candidate for industrial applications when optimal conditions are used to minimize the overall costs of the enzyme production process.
- Published
- 2024
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