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1. Peanut lipids influence the response of bronchial epithelial cells to the peanut allergens Ara h 1 and Ara h 2 by decreasing barrier permeability

Author

Palladino, Chiara, primary, Ellinger, Isabella, additional, Kalic, Tanja, additional, Humeniuk, Piotr, additional, Ret, Davide, additional, Mayr, Vanessa, additional, Hafner, Christine, additional, Hemmer, Wolfgang, additional, Hoffmann-Sommergruber, Karin, additional, Untersmayr, Eva, additional, Bublin, Merima, additional, Radauer, Christian, additional, and Breiteneder, Heimo, additional

Published
2023
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3. Pru du 1, the Bet v 1‐homologue from almond, is a major allergen in patients with birch pollen associated almond allergy

Author

Kabasser, Stefan, primary, Crvenjak, Nadja, additional, Schmalz, Stefanie, additional, Kalic, Tanja, additional, Hafner, Christine, additional, Dubiela, Pawel, additional, Kucharczyk, Aleksandra, additional, Bazan‐Socha, Stanislawa, additional, Lukaszyk, Mateusz, additional, Breiteneder, Heimo, additional, Radauer, Christian, additional, and Bublin, Merima, additional

Published
2022
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7. Development of a novel Ara h 2 hypoallergen with no IgE binding or anaphylactogenic activity

Author

Tscheppe, Angelika, Palmberger, Dieter, van Rijt, Leonie, Kalic, Tanja, Mayr, Vanessa, Palladino, Chiara, Kitzmüller, Claudia, Hemmer, Wolfgang, Hafner, Christine, Bublin, Merima, van Ree, Ronald, Grabherr, Reingard, Radauer, Christian, Breiteneder, Heimo, Ear, Nose and Throat, Experimental Immunology, AII - Inflammatory diseases, APH - Global Health, and APH - Personalized Medicine

Subjects
Adult, Male, Adolescent, Peanut allergy, T-Lymphocytes, mouse model, Lymphocyte Activation, Article, Mice, Ara h 2, Animals, Humans, Amino Acid Sequence, Child, Anaphylaxis, Infant, food and beverages, Antigens, Plant, Immunoglobulin E, Middle Aged, epitopes, Basophils, Child, Preschool, Mutation, Female, immunotherapy, hypoallergen, 2S Albumins, Plant
Abstract

Background: To date, no safe allergen-specific immunotherapy for patients with peanut allergy is available. Previous trials were associated with severe side effects. Objective: We sought to determine the relative importance of conformational and linear IgE-binding epitopes of the major peanut allergen Ara h 2 and to produce a hypoallergenic variant with abolished anaphylactogenic activity. Methods: Wild-type Ara h 2 and a mutant lacking the loops containing linear IgE epitopes were produced in insect cells. Conformational IgE epitopes were removed by unfolding these proteins through reduction and alkylation. IgE binding was tested by means of ELISA with sera from 48 Ara h 2–sensitized patients with peanut allergy. Basophil activation and T-cell proliferation were tested with blood samples from selected patients. Anaphylactogenic potency was tested by using intraperitoneal challenge of mice sensitized intragastrically to peanut extract. Results: Patients' IgE recognized conformational and linear epitopes in a patient-specific manner. The unfolded mutant lacking both types of epitopes displayed significantly lower IgE binding (median ELISA OD, 0.03; interquartile range, 0.01-0.06) than natural Ara h 2 (median ELISA OD, 0.99; interquartile range, 0.90-1.03; P < .01). Basophil activation by unfolded mutant Ara h 2 was low (median area under the curve, 72 vs 138 for native wild-type Ara h 2; P < .05), but its ability to induce T-cell proliferation was retained. Unfolded mutants without conformational epitopes did not induce anaphylaxis in peanut-sensitized mice. Conclusions: By removing conformational and linear IgE epitopes, a hypoallergenic Ara h 2 mutant with abolished IgE binding and anaphylactogenic potency but retained T-cell activation was generated.

Published
2020

8. Patients Allergic to Fish Tolerate Ray Based on the Low Allergenicity of Its Parvalbumin

Author

Kalic, Tanja, Morel-Codreanu, Francoise, Radauer, Christian, Ruethers, Thimo, Taki, Aya C, Swoboda, Ines, Hilger, Christiane, Hoffmann-Sommergruber, Karin, Ollert, Markus, Hafner, Christine, Lopata, Andreas L, Morisset, Martine, Breiteneder, Heimo, and Kuehn, Annette

Subjects
nervous system, Skin prick test, musculoskeletal, neural, and ocular physiology, Ray, mental disorders, Food challenge, natural sciences, IgE, Cod, Basophil activation, Fish allergy, Parvalbumin
Abstract

Background: Clinical reactions to bony fish species are common in patients with allergy to fish and are caused by parvalbumins of the β-lineage. Cartilaginous fish such as rays and sharks contain mainly α-parvalbumins and their allergenicity is not well understood. Objective: To investigate the allergenicity of cartilaginous fish and their α-parvalbumins in individuals allergic to bony fish. Methods: Sensitization to cod, salmon, and ray among patients allergic to cod, salmon, or both (n = 18) was explored by prick-to-prick testing. Clinical reactivity to ray was assessed in 11 patients by food challenges or clinical workup. IgE-binding to β-parvalbumins (cod, carp, salmon, barramundi, tilapia) and α-parvalbumins (ray, shark) was determined by IgE-ELISA. Basophil activation tests and skin prick tests were performed with β-parvalbumins from cod, carp, and salmon and α-parvalbumins from ray and shark. Results: Tolerance of ray was observed in 10 of 11 patients. Prick-to-prick test reactions to ray were markedly lower than to bony fish (median wheal diameter 2 mm with ray vs 11 mm with cod and salmon). IgE to α-parvalbumins was lower (median, 0.1 kU/L for ray and shark) than to β-parvalbumins (median, ≥1.65 kU/L). Furthermore, α-parvalbumins demonstrated a significantly reduced basophil activation capacity compared with β-parvalbumins (eg, ray vs cod, P

Published
2019

10. Molecular Immunology / WHO/IUIS Allergen Nomenclature : Providing a common language

Author

Pomés, Anna, Davies, Janet M., Gadermaier, Gabriele, Hilger, Christiane, Holzhauser, Thomas, Lidholm, Jonas, Lopata, Andreas L., Mueller, Geoffrey A., Nandy, Andreas, Radauer, Christian, Chan, Sanny K., Jappe, Uta, Kleine-Tebbe, Jörg, Thomas, Wayne R., Chapman, Martin D., van Hage, Marianne, van Ree, Ronald, Vieths, Stefan, Raulf, Monika, and Goodman, Richard E.

Subjects
immune system diseases, Isoallergen, otorhinolaryngologic diseases, IgE, respiratory system, WHO/IUIS Allergen Nomenclature, Allergen database, Taxonomic name, respiratory tract diseases
Abstract

A systematic nomenclature for allergens originated in the early 1980s, when few protein allergens had been described. A group of scientists led by Dr. David G. Marsh developed a nomenclature based on the Linnaean taxonomy, and further established the World Health Organization/International Union of Immunological Societies (WHO/IUIS) Allergen Nomenclature Sub-Committee in 1986. Its stated aim was to standardize the names given to the antigens (allergens) that caused IgE-mediated allergies in humans. The Sub-Committee first published a revised list of allergen names in 1986, which continued to grow with rare publications until 1994. Between 1994 and 2007 the database was a text table online, then converted to a more readily updated website. The allergen list became the Allergen Nomenclature database (www.allergen.org), which currently includes approximately 880 proteins from a wide variety of sources. The Sub-Committee includes experts on clinical and molecular allergology. They review submissions of allergen candidates, using evidence-based criteria developed by the Sub-Committee. The review process assesses the biochemical analysis and the proof of allergenicity submitted, and aims to assign allergen names prior to publication. The Sub-Committee maintains and revises the database, and addresses continuous challenges as new “omics” technologies provide increasing data about potential new allergens. Most journals publishing information on new allergens require an official allergen name, which involves submission of confidential data to the WHO/IUIS Allergen Nomenclature Sub-Committee, sufficient to demonstrate binding of IgE from allergic subjects to the purified protein. (VLID)2667976

Published
2018

11. Cor a 14, the allergenic 2S albumin from hazelnut, is highly thermostable and resistant to gastrointestinal digestion

Author

Pfeifer, Sabine, Bublin, Merima, Dubiela, Pawel, Hummel, Karin, Wortmann, Judith, Hofer, Gerhard, Keller, Walter, Radauer, Christian, and Hoffmann‐Sommergruber, Karin

Subjects
Hot Temperature, Protein Stability, Allergen, Molecular Sequence Data, Allergens, Antigens, Plant, Immunoglobulin E, Cor a 14, Protein Structure, Secondary, respiratory tract diseases, 2S albumin, Food allergy, Proteolysis, Humans, Protein Isoforms, Digestion, Amino Acid Sequence, Research Articles, Hazelnut (Corylus avellana), Research Article
Abstract

Scope Allergens from nuts frequently induce severe allergic reactions in sensitive individuals. The aim of this study was to elucidate the physicochemical characteristics of natural Cor a 14, the 2S albumin from hazelnut. Methods and results Cor a 14 was purified from raw hazelnuts using a combination of precipitation and chromatographic techniques. The protein was analyzed using gel electrophoresis, MS, and far‐UV circular dichroism (CD) analyses. The immunoglobulin E (IgE) binding of native, heat‐treated, and in vitro digested Cor a 14 was studied. We identified two different Cor a 14 isoforms and showed microclipping at the C‐terminus. CD spectra at room temperature showed the typical characteristics of 2S albumins, and temperatures of more than 80°C were required to start unfolding of Cor a 14 demonstrating its high stability to heat treatment. In vitro digestion experiments revealed that Cor a 14 is resistant to proteolytic degradation. Native and heat‐treated protein was recognized by sera from hazelnut allergic patients. However, denaturation of the allergen led to significantly reduced IgE binding. Conclusion We identified two different isoforms of Cor a 14 displaying high stability under heating and gastric and duodenal conditions. Data from IgE‐binding experiments revealed the existence of both, linear and conformational epitopes.

Published
2015

12. The Bet v 1 fold: an ancient, versatile scaffold for binding of large, hydrophobic ligands

Author

Breiteneder Heimo, Lackner Peter, and Radauer Christian

Subjects
Evolution, QH359-425
Abstract

Abstract Background The major birch pollen allergen, Bet v 1, is a member of the ubiquitous PR-10 family of plant pathogenesis-related proteins. In recent years, a number of diverse plant proteins with low sequence similarity to Bet v 1 was identified. In addition, determination of the Bet v 1 structure revealed the existence of a large superfamily of structurally related proteins. In this study, we aimed to identify and classify all Bet v 1-related structures from the Protein Data Bank and all Bet v 1-related sequences from the Uniprot database. Results Structural comparisons of representative members of already known protein families structurally related to Bet v 1 with all entries of the Protein Data Bank yielded 47 structures with non-identical sequences. They were classified into eleven families, five of which were newly identified and not included in the Structural Classification of Proteins database release 1.71. The taxonomic distribution of these families extracted from the Pfam protein family database showed that members of the polyketide cyclase family and the activator of Hsp90 ATPase homologue 1 family were distributed among all three superkingdoms, while members of some bacterial families were confined to a small number of species. Comparison of ligand binding activities of Bet v 1-like superfamily members revealed that their functions were related to binding and metabolism of large, hydrophobic compounds such as lipids, hormones, and antibiotics. Phylogenetic relationships within the Bet v 1 family, defined as the group of proteins with significant sequence similarity to Bet v 1, were determined by aligning 264 Bet v 1-related sequences. A distance-based phylogenetic tree yielded a classification into 11 subfamilies, nine exclusively containing plant sequences and two subfamilies of bacterial proteins. Plant sequences included the pathogenesis-related proteins 10, the major latex proteins/ripening-related proteins subfamily, and polyketide cyclase-like sequences. Conclusion The ubiquitous distribution of Bet v 1-related proteins among all superkingdoms suggests that a Bet v 1-like protein was already present in the last universal common ancestor. During evolution, this protein diversified into numerous families with low sequence similarity but with a common fold that succeeded as a versatile scaffold for binding of bulky ligands.

Published
2008
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14. 6th International Symposium on Molecular Allergology (ISMA)

Author

Hilger, Christiane, primary, Swiontek, Kyra, additional, Fischer, Jörg, additional, Hentges, François, additional, Lehners, Christiane, additional, Morisset, Martine, additional, Eberlein, Bernadette, additional, Biedermann, Tilo, additional, Ollert, Markus, additional, Wildner, Sabrina, additional, Stemeseder, Teresa, additional, Freier, Regina, additional, Briza, Peter, additional, Lang, Roland, additional, Batanero, Eva, additional, Villalba, Mayte, additional, Lidholm, Jonas, additional, Hawranek, Thomas, additional, Ferreira, Fatima, additional, Brandstetter, Hans, additional, Gadermaier, Gabriele, additional, Moingeon, Philippe, additional, Groeme, Rachel, additional, Bouley, Julien, additional, Bordas, Véronique, additional, Le Mignon, Maxime, additional, Bussières, Laetitia, additional, Lautrette, Aurélie, additional, Mascarell, Laurent, additional, Lombardi, Vincent, additional, Baron-Bodo, Véronique, additional, Chabre, Henri, additional, Batard, Thierry, additional, Nony, Emmanuel, additional, De Amicis, Karine Marafigo, additional, Watanabe, Alexandra Sayuri, additional, Figo, Daniele Danella, additional, Dos Santos-Pinto, José Roberto Aparecido, additional, Palma, Mario Sergio, additional, Castro, Fabio Fernandes Morato, additional, Kalil, Jorge, additional, Wohlschlager, Therese, additional, Ferreira-Briza, Fatima, additional, Santos, Keity Souza, additional, Faber, Margaretha, additional, Van Gasse, Athina, additional, Sabato, Vito, additional, Hagendorens, Margo M., additional, Bridts, Chris H., additional, De Clerck, Luc S., additional, Perales, Araceli Diaz, additional, Ebo, Didier, additional, Zavadakova, Petra, additional, Buchwalder, Aurélie, additional, Rebeaud, Fabien, additional, Märki, Iwan, additional, Gepp, Barbara, additional, Lengger, Nina, additional, Möbs, Christian, additional, Pfützner, Wolfgang, additional, Radauer, Christian, additional, Bohle, Barbara, additional, Galvao, Clovis Eduardo, additional, Santos-Pinto, Jose Roberto Aparecido, additional, Schwager, Christian, additional, Kull, Skadi, additional, Schocker, Frauke, additional, Behrends, Jochen, additional, Becker, Wolf-Meinhard, additional, Jappe, Uta, additional, Mastrorilli, Carla, additional, Tripodi, Salvatore, additional, Caffarelli, Carlo, additional, Asero, Riccardo, additional, Dondi, Arianna, additional, Ricci, Giampaolo, additional, Dascola, Carlotta Povesi, additional, Calamelli, Elisabetta, additional, Di Rienzo Businco, Andrea, additional, Bianchi, Annamaria, additional, Frediani, Tullio, additional, Verga, Carmen, additional, Iacono, Iride Dello, additional, Peroni, Diego, additional, Pingitore, Giuseppe, additional, Bernardini, Roberto, additional, Matricardi, Paolo Maria, additional, Hofer, Heidi, additional, Asam, Claudia, additional, Hauser, Michael, additional, Himly, Martin, additional, Ebner, Christof, additional, Lemoine, Pierrick, additional, Jain, Karine, additional, Abiteboul, Kathy, additional, Arvidsson, Monica, additional, Rak, Sabina, additional, Mota, Inês, additional, Garcia, Filipe Benito, additional, Gaspar, Angela, additional, Arêde, Cristina, additional, Piedade, Susana, additional, Sampaio, Graça, additional, Pires, Graça, additional, Borrego, Luís Miguel, additional, Santa-Marta, Cristina, additional, Morais-Almeida, Mário, additional, Popescu, Florin-Dan, additional, Vieru, Mariana, additional, Secureanu, Florin-Adrian, additional, Fernandes, Rosa Anita Rodrigues, additional, Carrapatoso, Isabel, additional, Gomes, Raquel, additional, Pereira, Celso, additional, Todo-Bom, Ana, additional, De Basoa, María Cecilia Martín Fernández, additional, Regio, Javier Barrios, additional, De Castro Cordova, Juan, additional, Ferreiro, Antón Fernández, additional, Tsilochristou, Olympia, additional, Perna, Serena, additional, Schwarz, Alina, additional, Rohrbach, Alexander, additional, Cappella, Antonio, additional, Hatzler, Laura, additional, Bauer, Carl-Peter, additional, Hoffmann, Ute, additional, Forster, Johannes, additional, Zepp, Fred, additional, Schuster, Antje, additional, D’amelio, Raffael, additional, Wahn, Ulrich, additional, Keil, Thomas, additional, Lau, Susanne, additional, Apoil, Pol André, additional, Mailhol, Claire, additional, Broué-Chabbert, Anne, additional, Juchet, Agnès, additional, Didier, Alain, additional, Carrer, Elodie, additional, Lanot, Thomas, additional, Blancher, Antoine, additional, Kurtaj, Almedina, additional, Hillebrand, Christoph, additional, Fichtinger, Gerda, additional, Danzer, Martin, additional, Gabriel, Christian, additional, Thalhamer, Theresa, additional, Scheiblhofer, Sandra, additional, Thalhamer, Josef, additional, Weiss, Richard, additional, Wolf, Martin, additional, Pichler, Ulrike, additional, Twaroch, Teresa, additional, Yokoi, Hidenori, additional, Takai, Toshiro, additional, Didierlaurent, Alain, additional, Mari, Adriano, additional, Behrendt, Heidrun, additional, Neubauer, Angela, additional, Stolz, Frank, additional, Ferreira, Fátima, additional, Wallner, Michael, additional, Carvalho, Sara, additional, Lourenço, Tatiana, additional, Cosme, Joana, additional, Duarte, Fátima Cabral, additional, Santos, Amélia Spínola, additional, Costa, Ana Célia, additional, Barbosa, Manuel Pereira, additional, Klinglmayr, Eva, additional, Schweidler, Bettina, additional, Lueftenegger, Lisa, additional, Moser, Stephanie, additional, Doppler, Patrick, additional, Oostingh, Gertie J., additional, Bathke, Arne, additional, Zumbach, Joerg, additional, Panzner, Petr, additional, Vachova, Martina, additional, Vlas, Tomas, additional, Maly, Marek, additional, Posa, Daniela, additional, Hofmaier, Stephanie, additional, Stock, Philippe, additional, Grabenhenrich, Linus, additional, Chen, Kuan-Wei, additional, Resch, Yvonne, additional, Vrtala, Susanne, additional, Valenta, Rudolf, additional, Abramidze, Tamar, additional, Lomidze, Nino, additional, Gotua, Maia, additional, Dapkeviciute, Austeja, additional, Einikyte, Ruta, additional, Norkuniene, Jolita, additional, Skrickiene, Laima, additional, Miskiniene, Asta, additional, Kvedariene, Violeta, additional, Schiener, Maximilian, additional, Moreno-Aguilar, Carmen, additional, Pietsch, Gunilla, additional, Intyre, Mareike Mc, additional, Schwarze, Lea, additional, Rußkamp, Dennis, additional, Spillner, Edzard, additional, Darsow, Ulf, additional, Schmidt-Weber, Carsten, additional, Blank, Simon, additional, Longé, Cyril, additional, Brazdova, Andrea, additional, Brunet, Jean-Louis, additional, Schwartz, Claire, additional, Girodet, Bruno, additional, Lavaud, François, additional, Birnbaum, Joelle, additional, Thi, Nhân Pham, additional, Duchateau, Magalie, additional, Chamot-Rooke, Julia, additional, Guilloux, Laurence, additional, Selva, Marie-Ange, additional, Couderc, Rémy, additional, Sénéchal, Hélène, additional, Sutra, Jean-Pierre, additional, Poncet, Pascal, additional, Augustin, Steffen, additional, Pump, Linda, additional, Wald, Martin, additional, Eichhorn, Thomas, additional, Fischer, Frank, additional, Willers, Christoph, additional, Miehe, Michaela, additional, Plum, Melanie, additional, Wolf, Sara, additional, Jabs, Frederic, additional, Raiber, Tim, additional, Bantleon, Frank, additional, Seismann, Henning, additional, Jakob, Thilo, additional, Apostolovic, Danijela, additional, Tran, Anh Thu, additional, Sanchez-Vidaurre, Sara, additional, Velickovic, Tanja Cirkovic, additional, Starkhammar, Maria, additional, Hamsten, Carl, additional, Van Hage, Marianne, additional, Dubiela, Pawel, additional, Humeniuk, Piotr, additional, Pfeifer, Sabine, additional, Bublin, Merima, additional, Borowski, Tomasz, additional, Hoffmann-Sommergruber, Karin, additional, Verschuren, Martie C. M., additional, Bastiaan-Net, Shanna, additional, Depoortere, Defien, additional, Foetisch, Kay, additional, Scheurer, Stephan, additional, Wichers, Harry J, additional, Noij, Theo, additional, Van Uden, Nikki M.E., additional, Vandenberghe, Karel, additional, Wichers, Harry J., additional, Noij, Theo H. M., additional, Roulias, Anargyros, additional, Parigiani, Maria Alejandra, additional, Ahammer, Linda, additional, Grutsch, Sarina, additional, Tollinger, Martin, additional, Moya, Raquel, additional, López-Matas, Mª Angeles, additional, Reyes, Raquel, additional, Carnés, Jerónimo, additional, Larré, Colette, additional, Rogniaux, Hélène, additional, Lupi, Roberta, additional, Denery-Papini, Sandra, additional, Pablos, Isabel Maria, additional, Eichhorn, Stephanie, additional, Machado, Yoan, additional, Park, Jung-Won, additional, Arora, Naveen, additional, Vieths, Stefan, additional, Tanaka, Charlene, additional, Pineau, Florence, additional, Drouet, Martine, additional, Beaudouin, Etienne, additional, Altenbach, Susan, additional, Mameri, Hamza, additional, Brossard, Chantal, additional, Gaudin, Jean Charles, additional, Moneret-Vautrin, Denise Anne, additional, Paty, Evelyne, additional, Tranquet, Olivier, additional, Masci, Stefania, additional, Moneret-Vautrin, Denise-Anne, additional, Petersen, Arnd, additional, Böttger, Marisa, additional, Rennert, Sandra, additional, Krause, Susanne, additional, Ernst, Martin, additional, Gutsmann, Thomas, additional, Bauer, Johann, additional, Lindner, Buko, additional, Koppelman, Stef, additional, Jayasena, Shyamali, additional, Luykx, Dion, additional, Schepens, Erik, additional, De Jong, Govardus, additional, Isleib, Tom, additional, Nordlee, Julie, additional, Baumert, Joe, additional, Taylor, Steve, additional, Maleki, Soheila, additional, Palladino, Chiara, additional, Sirvent, Sofía, additional, Angelina, Alba, additional, Eiwegger, Thomas, additional, Palomares, Oscar, additional, Breiteneder, Heimo, additional, Claude, Mathilde, additional, Bouchaud, Grégory, additional, Bodinier, Marie, additional, Korte, Robin, additional, Bräcker, Julia, additional, Brockmeyer, Jens, additional, Satoh, Rie, additional, Teshima, Reiko, additional, Tscheppe, Angelika, additional, Palmberger, Dieter, additional, Grabherr, Reingard, additional, Raith, Marianne, additional, Sonnleitner, Linda, additional, Zach, Doris, additional, Woroszylo, Konrad, additional, Focke-Tejkl, Margit, additional, Wank, Herbert, additional, Graf, Thorsten, additional, Kuehn, Annette, additional, Swoboda, Ines, additional, Huber, Sara, additional, Gay-Crosier, Fabienne, additional, Polak, Dominika, additional, Nagl, Birgit, additional, Kitzmüller, Claudia, additional, Samadi, Nazanin, additional, Geyeregger, Rene, additional, Jahn-Schmid, Beatrice, additional, Gomez, Ariel, additional, Haka, Jaana, additional, Hattara, Liisa, additional, Heikkinen, Marika, additional, Niemi, Merja H, additional, Rouvinen, Juha, additional, Saviranta, Petri, additional, Mattila, Pekka, additional, Takkinen, Kristiina, additional, Laukkanen, Marja-Leena, additional, Pablos, Isabel, additional, Kastner, Bianca, additional, Silar, Mira, additional, Selb, Julij, additional, Kogovsek, Rok, additional, Kosnik, Mitja, additional, Korosec, Peter, additional, Pestana, Leticia, additional, Melo, Alcinda Campos, additional, Mendes, Ana, additional, Pedro, Maria Elisa, additional, Santos, Maria Conceição Pereira, additional, Bienvenu, Françoise, additional, Goursaud, Claire, additional, Garnier, Lorna, additional, Jacquenet, Sandrine, additional, Degaud, Michaël, additional, Viel, Sébastien, additional, Barre, Annick, additional, Rougé, Pierre, additional, Bienvenu, Jacques, additional, Vitte, Joana, additional, Bensalah, Amel, additional, Cleach, Isabelle, additional, Mousseau, Laurent, additional, Agabriel, Chantal, additional, Liabeuf, Valérie, additional, Birnbaum, Joëlle, additional, Mège, Jean-Louis, additional, Gardner, James, additional, Gandhi, Minal, additional, Kariyawasam, Harsha, additional, Rotiroti, Giuseppina, additional, Regateiro, Frederico, additional, Faria, Emília, additional, Schmid, Johannes Martin, additional, Dahl, Ronald, additional, Hoffmann, Hans Juergen, additional, Pestana, Letícia, additional, Silva, Diana, additional, Vieira, Teresa, additional, Pereira, Ana Maria, additional, Moreira, André, additional, Delgado, Luís, additional, Prates, Sara, additional, Alves, Cátia, additional, Finelli, Elena, additional, Pinto, Paula Leiria, additional, Cardoso, Bárbara Kong, additional, Cruz, Cíntia, additional, Semedo, Filipa, additional, Tomaz, Elza, additional, Inácio, Filipe, additional, Maity, Santanu, additional, Baricevic-Jones, Ivona, additional, Marsh, Justin T., additional, Johnson, Phil E., additional, Balasundaram, Anuradha, additional, Hope, Anya-May, additional, Taekema, Aafke, additional, Simpson, Angela, additional, Semic-Jusufagic, Aida, additional, Clare Mills, E. N., additional, Nelly, Gourdon Dubois, additional, Laetitia, Sellam, additional, Bruno, Pereira, additional, Elodie, Michaud, additional, Khaled, Messaoudi, additional, Bertrand, Evrard, additional, Jean-Luc, Fauquert, additional, Goodman, Richard E., additional, Plata, Elena Rodríguez, additional, Amaral, Luis, additional, Bartolomé, Borja, additional, Coimbra, Alice, additional, Placido, Jose L, additional, Ganea, Carmen Saviana, additional, Costello, Carol Ann, additional, Sorensen, Martin, additional, Mills, Clare, additional, Rogers, Adrian, additional, Otherhals, Aage, additional, Kalic, Tanja, additional, Ellinger, Isabella, additional, Waltl, Eva, additional, Niederberger-Leppin, Verena, additional, Szczepankiewicz, Dawid, additional, Pruszynska-Oszmalek, Ewa, additional, Skrzypski, Marek, additional, Nowak, Krzysztof W., additional, Szczepankiewicz, Aleksandra, additional, Jang, Gwang-Cheon, additional, Markovic, Iva, additional, Borowski, Andreas, additional, Vetter, Tina, additional, Wohlmann, Andreas, additional, Kuepper, Michael, additional, Friedrich, Karlheinz, additional, Gracia, Ibon Eguiluz, additional, Bosco, Anthony, additional, Dollner, Ralph, additional, Melum, Guro Reinholt, additional, Jones, Anya C, additional, Lexberg, Maria, additional, Holt, Patrick G, additional, Bækkevold, Espen Sønderaal, additional, Jahnsen, Frode Lars, additional, Sobkowiak, Paulina, additional, Rachel, Marta, additional, Narozna, Beata, additional, Jenerowicz, Dorota, additional, Swiatowy, Witold, additional, Breborowicz, Anna, additional, Nestelbacher, Reinhard, additional, and Fukui, Hiroyuki, additional

Published
2016
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15. The major birch pollen allergen Bet v 1 induces different responses in dendritic cells of birch pollen allergic and healthy individuals

Author

Smole, Ursula, Radauer, Christian, Lengger, Nina, Svoboda, Martin, Rigby, Neil, Bublin, Merima, Gaier, Sonja, Hoffmann-Sommergruber, Karin, Jensen-Jarolim, Erika, Mechtcheriakova, Diana, and Breiteneder, Heimo

Subjects
Adult, Male, Transcription, Genetic, Science, Binding, Competitive, Monocytes, Th2 Cells, Hypersensitivity, Humans, Phosphorylation, Betula, Receptors, IgE, Dendritic Cells, Allergens, Antigens, Plant, Immunoglobulin E, Endocytosis, Enzyme Activation, Kinetics, Cross-Linking Reagents, Gene Expression Regulation, Case-Control Studies, Pollen, Medicine, Female, Mitogen-Activated Protein Kinases, Research Article
Abstract

Dendritic cells play a fundamental role in shaping the immune response to allergens. The events that lead to allergic sensitization or tolerance induction during the interaction of the major birch pollen allergen Bet v 1 and dendritic cells are not very well studied. Here, we analyzed the uptake of Bet v 1 and the cross-reactive celery allergen Api g 1 by immature monocyte-derived dendritic cells (iMoDCs) of allergic and normal donors. In addition, we characterized the allergen-triggered intracellular signaling and transcriptional events. Uptake kinetics, competitive binding, and internalization pathways of labeled allergens by iMoDCs were visualized by live-cell imaging. Surface-bound IgE was detected by immunofluorescence microscopy and flow cytometry. Allergen- and IgE-induced gene expression of early growth response genes and Th1 and Th2 related cytokines and chemokines were analyzed by real-time PCR. Phosporylation of signaling kinases was analyzed by Western blot. Internalization of Bet v 1 by iMoDCs of both donor groups, likely by receptor-mediated caveolar endocytosis, followed similar kinetics. Bet v 1 outcompeted Api g 1 in cell surface binding and uptake. MoDCs of allergic and healthy donors displayed surface-bound IgE and showed a pronounced upregulation of Th2 cytokine- and NFκB-dependent genes upon non-specific Fcε receptor cross-linking. In contrast to these IgE-mediated responses, Bet v 1-stimulation increased transcript levels of the Th2 cytokines IL-4 and IL-13 but not of NFκB-related genes in MoDCs of BP allergic donors. Cells of healthy donors were either unresponsive or showed elevated mRNA levels of Th1-promoting chemokines. Moreover, Bet v 1 was able to induce Erk1/2 and p38 MAPK activation in BP allergics but only a slight p38 activation in normal donors. In conclusion, our data indicate that Bet v 1 favors the activation of a Th2 program only in DCs of BP allergic individuals.

Published
2015

17. A Cross-Reactive Human Single-Chain Antibody for Detection of Major Fish Allergens, Parvalbumins, and Identification of a Major IgE-Binding Epitope

Author

Bublin, Merima, Kostadinova, Maria, Fuchs, Julian E., Ackerbauer, Daniela, Moraes, Adolfo H., Almeida, Fabio C. L., Lengger, Nina, Hafner, Christine, Ebner, Christof, Radauer, Christian, Liedl, Klaus R., Valente, Ana Paula, and Breiteneder, Heimo

Subjects
musculoskeletal, neural, and ocular physiology, lcsh:R, Fishes, lcsh:Medicine, Allergens, Cross Reactions, Immunoglobulin E, Epitopes, Parvalbumins, nervous system, mental disorders, Animals, Humans, lcsh:Q, lcsh:Science, Epitope Mapping, Food Hypersensitivity, Research Article, Single-Chain Antibodies
Abstract

Fish allergy is associated with moderate to severe IgE-mediated reactions to the calcium binding parvalbumins present in fish muscle. Allergy to multiple fish species is caused by parvalbumin-specific cross-reactive IgE recognizing conserved epitopes. In this study, we aimed to produce cross-reactive single chain variable fragment (scFv) antibodies for the detection of parvalbumins in fish extracts and the identification of IgE epitopes. Parvalbumin-specific phage clones were isolated from the human ETH-2 phage display library by three rounds of biopanning either against cod parvalbumin or by sequential biopanning against cod (Gad m 1), carp (Cyp c 1) and rainbow trout (Onc m 1) parvalbumins. While biopanning against Gad m 1 resulted in the selection of clones specific exclusively for Gad m 1, the second approach resulted in the selection of clones cross-reacting with all three parvalbumins. Two clones, scFv-gco9 recognizing all three parvalbumins, and scFv-goo8 recognizing only Gad m 1 were expressed in the E. coli non-suppressor strain HB2151 and purified from the periplasm. scFv-gco9 showed highly selective binding to parvalbumins in processed fish products such as breaded cod sticks, fried carp and smoked trout in Western blots. In addition, the scFv-gco9-AP produced as alkaline phosphatase fusion protein, allowed a single-step detection of the parvalbumins. In competitive ELISA, scFv-gco9 was able to inhibit binding of IgE from fish allergic patients' sera to all three β-parvalbumins by up to 80%, whereas inhibition by scFv-goo8 was up to 20%. 1H/15N HSQC NMR analysis of the rGad m 1:scFv-gco9 complex showed participation of amino acid residues conserved among these three parvalbumins explaining their cross-reactivity on a molecular level. In this study, we have demonstrated an approach for the selection of cross-reactive parvalbumin-specific antibodies that can be used for allergen detection and for mapping of conserved epitopes.

Published
2015

19. BMC Evolutionary Biology / The Bet v 1 fold : an ancient, versatile scaffold for binding of large, hydrophobic ligands

Author

Radauer, Christian, Lackner, Peter, and Breiteneder, Heimo

Subjects
chemical and pharmacologic phenomena, hemic and immune systems
Abstract

Background: The major birch pollen allergen, Bet v 1, is a member of the ubiquitous PR-10 family of plant pathogenesis-related proteins. In recent years, a number of diverse plant proteins with low sequence similarity to Bet v 1 was identified. In addition, determination of the Bet v 1 structure revealed the existence of a large superfamily of structurally related proteins. In this study, we aimed to identify and classify all Bet v 1-related structures from the Protein Data Bank and all Bet v 1-related sequences from the Uniprot database. Results: Structural comparisons of representative members of already known protein families structurally related to Bet v 1 with all entries of the Protein Data Bank yielded 47 structures with non-identical sequences. They were classified into eleven families, five of which were newly identified and not included in the Structural Classification of Proteins database release 1.71. The taxonomic distribution of these families extracted from the Pfam protein family database showed that members of the polyketide cyclase family and the activator of Hsp90 ATPase homologue 1 family were distributed among all three superkingdoms, while members of some bacterial families were confined to a small number of species. Comparison of ligand binding activities of Bet v 1-like superfamily members revealed that their functions were related to binding and metabolism of large, hydrophobic compounds such as lipids, hormones, and antibiotics. Phylogenetic relationships within the Bet v 1 family, defined as the group of proteins with significant sequence similarity to Bet v 1, were determined by aligning 264 Bet v 1-related sequences. A distance-based phylogenetic tree yielded a classification into 11 subfamilies, nine exclusively containing plant sequences and two subfamilies of bacterial proteins. ^Plant sequences included the pathogenesis-related proteins 10, the major latex proteins/ripening-related proteins subfamily, and polyketide cyclase-like sequences. Conclusion: The ubiquitous distribution of Bet v 1-related proteins among all superkingdoms suggests that a Bet v 1-like protein was already present in the last universal common ancestor. During evolution, this protein diversified into numerous families with low sequence similarity but with a common fold that succeeded as a versatile scaffold for binding of bulky ligands. SFB-F01802 (VLID)2211522

Published
2008

22. 95 Specific Recognition of the Major Birch Pollen Allergen BET V 1 Programs Dendritic Cells to Induce Either th2 or Tolerogenic Responses

Author

Smole, Ursula, primary, Balazs, Nina, additional, Radauer, Christian, additional, Sobanov, Yury, additional, Bublin, Merima, additional, Gaier, Sonja, additional, Hoffmann-Sommergruber, Karin, additional, Jensen-Jarolim, Erika, additional, Mechtcheriakova, Diana, additional, and Breiteneder, Heimo, additional

Published
2012
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26. A New Allergen from Ragweed (Ambrosia artemisiifolia) with Homology to Art v 1 from Mugwort

Author

Léonard, Renaud, primary, Wopfner, Nicole, additional, Pabst, Martin, additional, Stadlmann, Johannes, additional, Petersen, Bent O., additional, Duus, Jens Ø., additional, Himly, Martin, additional, Radauer, Christian, additional, Gadermaier, Gabriele, additional, Razzazi-Fazeli, Ebrahim, additional, Ferreira, Fatima, additional, and Altmann, Friedrich, additional

Published
2010
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28. NADP-dependent Mannitol Dehydrogenase, a Major Allergen of Cladosporium herbarum

Author

Simon-Nobbe, Birgit, primary, Denk, Ursula, additional, Schneider, Peter Bernhard, additional, Radauer, Christian, additional, Teige, Markus, additional, Crameri, Reto, additional, Hawranek, Thomas, additional, Lang, Roland, additional, Richter, Klaus, additional, Schmid-Grendelmeier, Peter, additional, Nobbe, Stephan, additional, Hartl, Arnulf, additional, and Breitenbach, Michael, additional

Published
2006
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30. Chimeras of Bet v 1 and Api g 1 reveal heterogeneous IgE responses in patients with birch pollen allergy

Author

Gepp, Barbara, Lengger, Nina, Bublin, Merima, Hemmer, Wolfgang, Breiteneder, Heimo, and Radauer, Christian

Subjects
Api g 1.0101, IgE epitope mapping, Immunology, Immunology and Allergy, chemical and pharmacologic phenomena, hemic and immune systems, Bet v 1.0101, chimera-based technology, patient-specific IgE repertoire, birch pollen allergy
Abstract

BackgroundCharacterization of IgE-binding epitopes of allergens and determination of their patient-specific relevance is crucial for the diagnosis and treatment of allergy.ObjectiveWe sought to assess the contribution of specific surface areas of the major birch pollen allergen Bet v 1.0101 to binding IgE of individual patients.MethodsFour distinct areas of Bet v 1 representing in total 81% of its surface were grafted onto the scaffold of its homolog, Api g 1.0101, to yield the chimeras Api-Bet-1 to Api-Bet-4. The chimeras were expressed in Escherichia coli and purified. IgE binding of 64 sera from Bet v 1–sensitized subjects with birch pollen allergy was determined by using direct ELISA. Specificity was assessed by means of inhibition ELISA.ResultsrApi g 1.0101, Api-Bet-1, Api-Bet-2, Api-Bet-3, and Api-Bet-4 bound IgE from 44%, 89%, 80%, 78%, and 48% of the patients, respectively. By comparing the amount of IgE binding to the chimeras and to rApi g 1.0101, 81%, 70%, 75%, and 45% of the patients showed significantly enhanced IgE binding to Api-Bet-1, Api-Bet-2, Api-Bet-3, and Api-Bet-4, respectively. The minority (8%) of the sera revealed enhanced IgE binding exclusively to a single chimera, whereas 31% showed increased IgE binding to all 4 chimeras compared with rApi g 1.0101. The chimeras inhibited up to 70% of IgE binding to rBet v 1.0101, confirming the specific IgE recognition of the grafted regions.ConclusionThe Bet v 1–specific IgE response is polyclonal, and epitopes are spread across the entire Bet v 1 surface. Furthermore, the IgE recognition profile of Bet v 1 is highly patient specific.

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