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Background: Microalbuminuria is an early sign of kidney disease in people with diabetes and indicates increased risk of cardiovascular disease. We tested whether a urinary proteomic risk classifier (CKD273) score was associated with development of microalbuminuria and whether progression to microalbuminuria could be prevented with the mineralocorticoid receptor antagonist spironolactone. Methods: In this multicentre, prospective, observational study with embedded randomised controlled trial (PRIORITY), we recruited people with type 2 diabetes, normal urinary albumin excretion, and preserved renal function from 15 specialist centres in ten European countries. All participants (observational cohort) were tested with the CKD273 classifier and classified as high risk (CKD273 classifier score >0·154) or low risk (≤0·154). Participants who were classified as high risk were entered into a randomised controlled trial and randomly assigned (1:1), by use of an interactive web-response system, to receive spironolactone 25 mg once daily or matched placebo (trial cohort). The primary endpoint was development of confirmed microalbuminuria in all individuals with available data (observational cohort). Secondary endpoints included reduction in incidence of microalbuminuria with spironolactone (trial cohort, intention-to-treat population) and association between CKD273 risk score and measures of impaired renal function based on estimated glomerular filtration rate (eGFR; observational cohort). Adverse events (particularly gynaecomastia and hyperkalaemia) and serious adverse events were recorded for the intention-to-treat population (trial cohort). This study is registered with the EU Clinical Trials Register (EudraCT 20120-004523-4) and ClinicalTrials.gov (NCT02040441) and is completed. Findings: Between March 25, 2014, and Sept 30, 2018, we enrolled and followed-up 1775 participants (observational cohort), 1559 (88%) of 1775 participants had a low-risk urinary proteomic pattern and 216 (12%) had a high-risk pattern, of whom 209 were included in the trial cohort and assigned to spironolactone (n=102) or placebo (n=107). The overall median follow-up time was 2·51 years (IQR 2·0–3·0). Progression to microalbuminuria was seen in 61 (28%) of 216 high-risk participants and 139 (9%) of 1559 low-risk participants (hazard ratio [HR] 2·48, 95% CI 1·80–3·42; p

A sample of 1107 fish served to age and to analyze growth of European conger eel (Conger conger) from NE Atlantic Ocean using otoliths and vertebrae.

Twenty-six isolates obtained from soybean crops (Glycine max) with typical anthracnose symptoms were identified as Colletotrichum truncatum (73 %) and C. destructivum (26 %). Their genetic relationships were studied using the AFLP method. A UPGMA phenogram divided the strains into two clusters corresponding with the two species. Genetic distances based on association coefficient were 0.71-0.89 among the 18 C. truncatum strains and 0.67-1 among the eight C. destructivum strains. Genetic variability within species, measured in terms of percentage of polymorphic loci, was high (

Simpósio Nacional de Olivicultura, 5º, Santarém, 24-26 de Setembro, 2009 Phenological development of the olive tree is important for studying its adaptability, for management and simulation of growth and yield of olive orchards. Nevertheless, most studies that predict the date of flowering of the olive do not consider its chilling requirements, that are low, but may not be satisfied in some years, under mild climates or when global warming takes effect. In a previous study De Melo-Abreu et al. (2004) a model designed to predict the date of flowering was presented along with appropriate parameters for some cultivars. It is a two phase model. In the first phase, chilling accumulation takes place using a four parameter sub-model for determination of chilling units. In the second phase, known as the forcing phase, that starts after chilling requirements were attained and bud-break was due to occur, temperature sum above a base temperature is used to simulate development. Furthermore, it was established that all the parameters for the chilling accumulation, except the required chilling units, are conservative for all cultivars that were studied. A custom-tailored algorithm was constructed for the determination of the total sum of chilling units for bud-break and for the temperature sum necessary for the forcing phase. The resulting calibrated model is used for the prediction of the impact of global warming in the dates of flowering of the olive trees ‘Arbequina’, ‘Gordal’, ‘Hojiblanca’, ‘Manzanilla’, ‘Moraiolo’, ‘Picual’ and ‘Verdial’ for the four locations that are representative of the main olive producing regions in Portugal. The present climate is represented by a series of 19 and 30 years of maximum and minimum daily temperatures (Cen0). Three scenarios of climate change were considered. Cen1, Cen2 and Cen3 correspond, respectively, to increases of 1°C, 2°C and 3°C to both maximum and minimum temperaturesIn Vila Real, projections indicate successive advances in the date of flowering, from 11 to 12 days in Cen1 until 33 to 37 days in Cen3. In Castelo Branco, the projections indicate advances of flowering date from 11 to 13 days in Cen1 until 23 to 36 days in Cen3. In both locations, no faulty of abnormal flowering were anticipated. In Beja, the o projections indicate that advances in the date of flowering for Cen1 and Cen2 are modest, that ‘Moraiolo’ has a single event of faulty or abnormal flowering in Cen2 and that all cultivars that were studied have years with faulty or abnormal flowerings in Cen3. In Faro, important delays in the date of flowering were anticipated in all scenarios, and in many years flowering is absent or abnormal. In Cen3, the projections indicate that flowering is likely to be absent or abnormal in almost all years. The results that were obtained are an indication that may help the farmer to include or reject cultivars in future olive orchards.

Pedosedimentary processes, recorded in a late Quaternary section near Zarate city, north Buenos Aires province are reconstructed in this study. Both field features and laboratory results were analyzed as a whole. Grain size, sand, silt and clay mineralogy, micromorphology, Rb and Sr concentrations, optical stimulated luminescence (OSL) ages as well as preliminary paleomagnetic analysis were performed in order to get a detailed information on processes involved. All studied paleosols were developed on loess horizons. Four definite breaks in sedimentation and a coupled development of paleosols were observed. On this basis four units showing different types of calcretes were defined. Welded paleosols at unit C, could be correlated to those studied at Baradero (Buenos Aires province) and Lozada (Córdoba province), which probably record the last interglacial stage before present, the Marine Isotopic Stage 5 (MIS5), studied on marine cores. The highest values of Rb/Sr ratio reflect changes due to chemical weathering processes and generally match paleosol levels occurrences. Development of ground water-related and pedogenic calcretes, as well as their association to smectite, illite and ferric oxide indicate drier weather conditions than present and strong seasonality. Finally, a tentative correlation between one of the paleosols and the marine isotope stage 11(MIS 11) is suggested. Fil:Tófalo, O.R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Orgeira, M.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Ramos, A.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Alonso, M.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.

Phytophthora sojae causes root and stem rot, one of the most important diseases of soybean worldwide. Genetic diversity of 32 Phytophthora sojae isolates of different geographic origin from Argentina was evaluated with RAPD markers. The isolates were collected from diseased soybean plants and soil samples from Santa Fe, Buenos Aires, Córdoba and Entre Ríos provinces, in the Pampeana Region. DNA was amplified with 20 decanucleotides primers. Seven primers amplified 49 fragments, of which 35 were polymorphic, indicating high variability. RAPD analysis detected intraspecific variability even among isolates of the same geographic origin. © 2007 by The Mycological Society of America. Fil:Gally, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Ramos, A.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Dokmetzian, D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Lopez, S.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.