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Zadovoljstvo zaposlenih pri delu je eden izmed tistih ključnih dejavnikov, ki vodi do organizacijskega uspeha in na katerega so dandanes pozorne praktično vse organizacije. Vedno bolj se poudarja pomen zadovoljstva zaposlenih pri delu, saj ta omogoča veliko pozitivnih učinkov tako za organizacijo kot tudi za zaposlene. Večja produktivnost (storilnost), boljši medosebni odnosi, manjša fluktuacija in s tem manjši stroški so le nekateri izmed teh učinkov. Poznavanje dejavnikov, ki vplivajo na zadovoljstvo zaposlenih pri delu pomaga identificirati razloge in področja, s katerimi in na katerih zaposleni niso zadovoljni. Na osnovi tega lahko organizacija uvede določene spremembe in prilagoditve ter tako izboljša raven zadovoljstva zaposlenih pri delu znotraj organizacije. Namen magistrskega dela je teoretično osvetliti področje delovnega okolja in z izvedbo raziskave v izbrani organizaciji državne uprave in izbrani organizaciji zasebnega sektorja ugotoviti, kako dejavniki delovnega okolja vplivajo na zadovoljstvo zaposlenih pri delu ter oblikovati priporočila za uvedbo sprememb v izbranih organizacijah. V magistrskem delu je bila izvedena mednarodna primerjava sekundarnih podatkov štirih držav. Izvedena je bila analiza izbranih dejavnikov kakovosti delovnega okolja, ki se meri tako s subjektivnimi ocenami zaposlenih kot z objektivnimi pokazatelji. Z metodo anketiranja je bila izvedena tudi raziskava na vzorcu dveh izbranih organizacij javnega in zasebnega sektorja. Osredotočilo se je predvsem na oceno dejavnikov zadovoljstva z delom in delovnim mestom ter na zaznavo dejavnikov delovnega okolja. Rezultati mednarodne primerjave kažejo, da med spremljanimi dejavniki delovnega okolja v Sloveniji, Avstriji, Nemčiji in na Danskem ni bistvenih razlik ter da so razmere za delo v vseh državah varne. Rezultati anketne raziskave so pokazali, da so sodelujoči v raziskavi v obeh sektorjih najbolj zadovoljni z ožjimi sodelavci, s katerimi delajo, medtem ko so najmanj zadovoljni z intenzivnostjo nagrajevanja, ki so je deležni. Obstaja kar nekaj statistično značilnih razlik med skupinama zaposlenih iz javnega in zasebnega sektorja, in sicer so povprečno višje ocene zadovoljstva zaposlenih pri delu v skupini iz zasebnega sektorja. Na podlagi rezultatov raziskave se predlaga, da vodje storijo več za dvig motiviranosti zaposlenih in za izboljšanje delovnega okolja, kar posledično vpliva tudi na višje zadovoljstvo zaposlenih pri delu in večjo uspešnost organizacij. Employee satisfaction at work is one of those key factors that enable organizational success and to which virtually all organizations pay attention today. The importance of job satisfaction is increasingly emphasized because it provides many positive effects for both the organization and employees. Higher productivity (performance), better interpersonal relationships, less fluctuation, and, thus, lower costs are just some of these effects. Knowing the factors that affect employee satisfaction at work helps to identify the reasons and areas with which and in which employees are dissatisfied. Based on this, the organization can introduce certain changes and adjustments. Thus, it improves the level of satisfaction within the organization. The purpose of the master's thesis is to highlight the field of work environment theoretically and, by researching a selected state administration organization and a selected private sector organization, determine how work environment factors affect employee satisfaction at work, as well as to make recommendations for changes in the selected organizations. In the master's thesis, an international comparison of secondary data from four countries was performed. An analysis of the selected factors of the quality of the working environment was performed. It is measured both by subjective assessments of employees and by objective indicators. A survey was also conducted on a sample of the two selected public and private sector organizations using the survey method. It focused mainly on the assessment of job satisfaction and satisfaction-with-work factors and the perception of work environment factors. The results of the international comparison show that there are no significant differences between the monitored factors of the work environment in Slovenia, Austria, Germany, and Denmark and that the working conditions in all countries are safe. The results of the survey showed that survey participants in both sectors are most satisfied with the close associates they work with while they are least satisfied with the intensity of rewards they receive. There are quite a few statistically significant differences between the groups of employees from the public and private sectors, i.e. on average, there are higher satisfaction ratings in the group of employees from the private sector. Based on the results of the research, it is proposed that managers do more to increase employee motivation and to improve the work environment, which, in turn, affects also higher employee satisfaction and greater performance of organizations.

Elektroporacija (EP) je fizikalna metoda s katero z dovajanjem električnih pulzov v celični membrani za kratek čas ustvarimo pore, kar olajša prehod v celice določenim molekulam. Za zdravljenje rakavih obolenj se vse bolj uporablja EP v kombinaciji s cisplatinom (CDDP) in bleomicinom, čemur rečemo elektrokemoterapija (EKT). Cisplatin je platinova (II) spojina, ki s kovalentnimi povezavami povzroči spremembe v strukturi DNA in vodi v smrt rakave celice. Kljub temu, da je CDDP eden najpogosteje uporabljenih kemoterapevtikov, pa povzroča hude stranske učinke in tudi odpornost rakavih celic. Zaradi tega se raziskave usmerjajo v razvoj novih platinovih (II) analogov s protitumorskim delovanjem, kamor spadata tudi [PtCl (5,7-dibromo-8-hidroksikinolinato)(S-dmso)] (spojina A) in njen analog, [PtCl (5,7-dibromo-8-hidroksikinolinato)(pta)] (spojina B). Citotoksičnost obeh spojin v kombinaciji z EP smo preverili na humani celični liniji raka debelega črevesja (HT29) in raka dojke (MCF7). S testom klonogenosti smo izračunali vrednosti IC50 in jih primerjali z vrednostmi IC50 CDDP, s pripravo citospinov in barvanjem po Giemsi pa smo na podlagi morfoloških značilnosti ugotovili delež apoptotičnih in nekrotičnih celic. Ugotovili smo, da spojini A in B delujeta citotoksično na tumorske celice in povzročata predvsem apoptozo ter deloma nekrozo. Elektroporacija statistično značilno poveča citotoksično delovanje vseh treh platinovih (II) spojin in je učinkovita metoda za izboljšanje vnosa ne samo CDDP, pač pa tudi spojine A in B. Spojina B se je v kombinaciji z in brez EP izkazala za bolj toksično od spojine A in hkrati tudi enako ali celo bolj citotoksično kot CDDP pri obeh celičnih linijah in je zato primerna za nadaljne testiranje, kot potencialni kemoterapevtik. Electroporation (EP) is a physical technique in which an electric field is applied to cells in order to increase the permeability of cell membrane for poorly permeating molecules. Electroporation in combination with cisplatin (CDDP), also called electrochemotherapy (ECT), is used for local treatment of several malignant tumors. Cisplatin is a well-known platinum (II) antitumor drug which antitumor activity has been linked to its ability to form DNA adducts, which subsequently lead to apoptosis of cancer cells. Although CDDP is broadly used, its main limitations are severe side effects and tumor cells’ resistance. Because of this, researchers are focusing on developing new platinum (II) complexes with antitumor activity, including [PtCl (5,7-dibromo-8-hydroxyquinolinato)(S-dmso)] (compound A) and it's analog, [PtCl (5,7-dibromo-8-hydroxyquinolinato)(pta)] (compound B). In the thesis, we analysed the cytotoxicity of compound A and B in combination with EP in human colon cancer (HT29) and human breast cancer cell line (MCF7). We determined IC50 values with clonogenic assay and compared it to IC50 values of CDDP. We used Giemsa staining to determine the percentage of apoptotic and necrotic cells. Results show that all compounds, A, B and CDDP have a cytotoxic effect on both tumor cell lines and cause primarily apoptotic cell death. Furthermore, EP has proved to be a very effective method for drug uptake as it has significantly improved cytotoxicity of all three platinum (II) complexes. Compound B has turned out to be more cytotoxic with and without EP than compound A and showed similar or better cytotoxicity than CDDP in both cell lines. Based on our results, compound B is very promising potential therapeutic agent.