Search

Rationale: ALL is the most common form of leukemia (75% to 80%), it is characterized by clonal expansion of the lymphoid blasts in bone marrow, blood, and other tissues, which can be divided into T lineage and B lineage. Although relapse of acute leukemia is common, a change of immunophenotype at relapse only occurs rarely. Some of these cases have been labeled “lineage switch”. Patient concerns: A 31-year-old man had multiple lymph nodes in the neck, and the lymph nodes on the right side adhered to the surrounding tissues. His lymphocytes ratio in blood was up to 86.3%. Flow cytometry of the bone marrow aspirate showed positive results for CD2, CD5, CD7, cCD3, TDT, CD4, CD8, and CD10, negative results for CD34, CD117, CD33, HLA-DR, CD19, and CD20. Twenty six months later, the patient felt pain in the neck and shoulder after touching. His lymphocytes of blood were 109.9×109 /L. 43 fusion genes and positive BCR/ABL was detected. Flow cytometry of the bone marrow aspirate showed pro B lymphocytes accounted for 85.54%, and positive expression of CD38, CD10, CD34, CD33, TDT, CD9, and HLA-DR. Moreover, the RT-PCR data showed the patient expressed high level of T cell and B cell development transcription factors. Diagnoses: Upon examination, the patient was initially diagnosed with T-lineage pro cell ALL. BM morphologic analysis presented complete remission (CR) after systemic chemotherapy. Twenty six months later, we discovered the patient was diagnosed with B-lineage acute lymphocytic leukemia. Interventions: Systemic chemotherapy is first given when a patient was diagnosed with T-cell acute lymphoblastic leukemia. After the patient happened linage switch, we adjusted the treatment plan, and the patient was complete remission after 1 course of treatment. Outcomes: Our case provides information of lineage switch from T-ALL to B-ALL in this report, which is never seen in our knowledge. Lessons: This lineage switch from T-ALL to B-ALL is never reported beforemoreover, the RT-PCR data showed the patient expressed high level of T cell and B cell development transcription factors. Its early recognition can let doctor provides appropriate therapy to patient.

eIF4E plays an important role in regulating tumor growth and angiogenesis, and eIF4E is highly expressed in a variety of lung cancer cell lines. siRNA eIF4E can significantly inhibit the proliferation of lung cancer cells, indicating that inhibition of eIF4E may become a novel anti-tumor target. In the previous study, we synthesized a series of small molecule compounds with the potential to inhibit eIF4E. Among them, the compound EGPI-1 significantly inhibited the proliferation of a variety of lung cancer cells such as A549, NCI-H460, NCI-H1650 and 95D without inhibiting the proliferation of HUVEC cells. Further studies found that EGPI-1 interfered with the eIF4E/eIF4G interaction and inhibited the phosphorylation of eIF4E in NCI-H460 cells. The results of flow cytometry showed that EGPI-1 induced apoptosis and G0/G1 cycle arrest in NCI-H460 cell. Interestingly, we also found that EGPI-1 induced autophagy and DNA damage in NCI-H460 cells. The mechanism results showed that EGPI-1 inhibited the Ras/MNK/ERK/eIF4E signaling pathway. Moreover, EGPI-1 inhibited tube formation of HUVECs, as well as inhibited the neovascularization of CAM, proving the anti-angiogenesis activity of EGPI-1. The NCI-H460 xenograft studies showed that EGPI-1 inhibited tumor growth and angiogenesis in vivo by regulating Ras/MNK/ERK/eIF4E pathway. Our studies proved that eIF4E was a novel target for regulating tumor growth, and the eIF4E/eIF4G interaction inhibitor EGPI-1 was promising to develop into a novel anti-lung cancer drug.

Rhodium phosphide (RhP) is utilized as a novel cocatalyst for CdS photocatalyst. The RhP cocatalyst greatly improves the photocatalytic performance, reusability and lifetime of CdS for H2 generation from splitting water under visible light illumination (λ > 420 nm). The optimal compound, 20 wt% RhP/CdS, produces the highest hydrogen amount of 325.4 μmol/mg after 10 h, which is about 196,024 times that of pure CdS. The apparent quantum yield of H2 evolution over the 20 wt% RhP/CdS after 100 h reaches 34.3%. Ultraviolet photoelectron spectrometer, fluorescence spectroscopy and photocurrent show that the RhP with strong electron attraction ability can accelerate the separation of photo-excited electron-hole pairs, which is responsible for the enhanced hydrogen evolution ability of CdS.

Litter size is an important economic traits in pigs. SLA-11 gene is a member of SLA (swine leukocyte antigen) complex. In our previous study, the SLA-11 gene was differentially expressed in PMSG-hCG stimulated preovulatory ovarian follicles of Chinese Taihu and Large White sows. Here, we identified two mutations (c.754-132 T > C and c.1421 + 38 T > C) in SLA-11 gene and analyzed the associations of two SNPs with litter size traits in Large White (n = 263) and DIV (n = 117) sows. The results showed that in Large White pigs, SLA-11 c.754-132 CC sows produced 0.74 and 0.87 more pigs per litter for TNB and NBA of all parities than did TT sows (p

The topological structures of complex networks play a crucial role in determining their evolutionary mechanisms and functional behaviors, and may have significant consequences for many real-world applications. Many researchers focused on the geometric features, collective behaviors and control of complex networks provided with precisely known structures. However, the exact topology of a network is usually unknown or uncertain in practical situations. Therefore, accurate and timely topology identification is of great necessity and importance. This paper presents a novel scheme for topology identification. Specifically, an auxiliary complex network with a very general form is constructed and some adaptive controllers are designed to recover the topology of the considered network upon generalized outer synchronization. Different from previous schemes, the network constructed can be composed of any kind of nodes. If the network with an unknown topology has very complicated node dynamics or a high node dimension, one can construct a response network consisting of nodes with much simpler dynamics, which is very practical for circuit design. The effectiveness of the theoretical findings has been illustrated by three numerical examples. This work provides a convenient approach to recovering network topology, which can facilitate the selection of practical circuits and reduce application costs.

Bi-doped Ag 3 PO 4 was synthesized by an ion exchange method, and its structure and properties were characterized systematically. Doping Bi 3+ ions into the crystal lattice of Ag 3 PO 4 changed the properties of particles, reduced the valence band position and widened the band gap of Ag 3 PO 4 . The doped Bi 3+ ions have replaced P 5+ ions of P–O tetrahedrons according to the XRD and XPS analysis. The testing of methyl orange (MO) photodegradation showed that 2 wt% Bi-doped Ag 3 PO 4 had the highest activity, with the degradation rate reaching 90.7% after 6 min (1 g/L catalyst) when pure Ag 3 PO 4 degraded only 27.3% of MO. Its degradation rate constant was 4.5 times that of undoped Ag 3 PO 4 . Particularly, 2 wt% Bi-doped Ag 3 PO 4 managed to photodegrade high-concentration (40 mg/L) MO (1 g/L catalyst), with the degradation rate constant 7.3 times that of undoped Ag 3 PO 4 . •OH radicals were the main active species in the oxidation of MO over Bi-doped Ag 3 PO 4 . Doping Bi 3+ substantially reduced OH defects on the surface of Ag 3 PO 4 , which significantly increased the degradation efficiency of MO over Ag 3 PO 4 . In addition, the density-functional theory calculation revealed that the influence of Bi dopants on the electronic structure may be an important reason for the enhanced photocatalytic performance of Ag 3 PO 4 .

A new AgSCN photocatalytic material with a novel nonmetal acid structure was synthesized by an ion-exchange method. Its structure and properties were characterized in detail. UV–vis absorption spectrum showed that AgSCN had the optical indirect band gap of 3.51 eV. The valence level of AgSCN was determined as 1.52 eV by X-ray photoelectron spectrometer. The photodegradation efficiency of rhodamine B over AgSCN was 2.74 times that of N-doped TiO2 (N-TiO2) under UV light radiation. The photodegradation rate constant was 4.8 times that of N-TiO2. The high photocatalytic activity mainly resulted from SCN− and the high position of valence band. OH radicals, as the dominant active species in photocatalysis, primarily originated from the multi-step reduction of O2 by photogenerated electron.

The Ag3PO4 and Ag3PO4 sonocatalysts with surface enrichment of Br− anions (Br–Ag3PO4) were synthesized using a facile precipitate process. Various analysis, including X-ray diffraction, transmission electron microscopy, scanning electron microscope, Brunauer–Emmett–Teller, photoluminescence (PL) spectra, and UV–Vis absorption spectra were carried out to investigate the property and structure. The effect of a variety of changing conditions on ultrasonic degradation of organic dye has been investigated. Both of them exhibited obvious activity for sonocatalytic degradation of organic dyes. However, Br–Ag3PO4 sonocatalysts showed much higher activity than that of Ag3PO4. By XRD analysis, the Br− anions of the surface of Ag3PO4 has been transformed into AgBr in the preparation process. The high activity could be attributed to AgBr for modifying Ag3PO4. By detecting hydroxyl radicals (·OH) in the reactive solution, we found that AgBr on the surface of Ag3PO4 can help to produce a lot of additional ·OH radicals. These ·OH radicals may be significant reason to improve Ag3PO4 activity, which further proved the ·OH radical mechanism of Ag3PO4 degradation of dyes under ultrasonic radiation. In addition, kinetic analysis and sonocatalytic mechanism were discussed in detail.

AgBr sonocatalysts were prepared by a simple method. The AgBr sonocatalysts exhibited excellent sonocatalytic activities against the decolorization of methylene blue, rhodamine B, and methyl orange. By determining the content of OH radicals in the ultrasonic degradation of organic dyes, many OH radicals were detected, which may be responsible for the high sonodegradation rate over AgBr under ultrasonic radiation. Based on the effects of the initial dye concentration on the sonodegradation, we also discussed and analyzed the kinetic data, and the results are consistent with the first-order kinetic rate equation.

A new catalyst of silver tyipolyphosphate (Ag5P3O10) for sonodegradation of dyes, was prepared by a hydrothermal route. The samples were characterized by X-ray diffraction (XRD), scanning electron microscopy (SEM), transmission electron microscopy (TEM), UV–vis absorption spectroscopy (UV–vis), photoluminescence spectra (PL) and N2 adsorption–desorption methods. The degradation of dyes in water was investigated under ultrasonic irradiation in the presence of Ag5P3O10. The result shows Ag5P3O10 has the excellent sonodegradation activity of dyes. A large number of hydroxyl ( OH) radicals formed under ultrasonic radiation, and the content of OH radicals increased with increased radiation time. This phenomenon provided the main basis for the high sonodegradation activity of Ag5P3O10 under ultrasonic radiation. The kinetic model and its parameters were also discussed in detail.

A composite semiconductor AgBr/Ag5P3O10 heterojunction has been synthesized by a simple one-step ion-exchange process in Br ionic liquids. All the as-obtained AgBr/Ag5P3O10 heterojunctions were characterized by X-ray powder diffraction (XRD), scanning electronic microscopy (SEM), transmission electronic microscopy (TEM), Brunauer–Emmett–Teller (BET), UV–vis absorption spectroscopy (UV–vis), photoluminescence (PL), and surface photovoltage (SPS) techniques. The as-prepared AgBr/Ag5P3O10 heterojunctions intensively absorb in the visible light region. The effects of different conditions on the photocatalytic properties were investigated systematically. The AgBr/Ag5P3O10 heterojunctions exhibited an enhanced photocatalytic activity than the individual Ag5P3O10 did in the degradation of methylene blue (MB) under visible-light irradiation. Much more OH radicals were found in the AgBr/Ag5P3O10 system in comparison with those in Ag5P3O10 alone by analyzing the formed OH radicals under visible light radiation. The SPS measurement shows that the SPS amplitude of AgBr/Ag5P3O10 heterojunctions was higher than that of Ag5P3O10, which indicates the higher charge separation efficiency of AgBr/Ag5P3O10 heterojunctions. Based on the above experimental results, the kinetic data and the photocatalytic mechanism of the AgBr/Ag5P3O10 heterojunctions were discussed in detail.

Highly ordered Mg(OH) 2 nanotube arrays were prepared inside the pores of porous anodic alumina membranes (AAM) by a simple chemical deposition method. The obtained Mg(OH) 2 /Al 2 O 3 composite membranes were used to remove nickel ions from wastewater with high removal efficiency. Furthermore, after heating the membranes that contained initial absorbed matter, the resulted MgO/NiO/Al 2 O 3 metal-oxides nanostructures still showed nice performance of removing Ni 2+ . This composite AAM system and its preparation on a large scale have been discussed, showing potential application in treatment of wastewaters containing heavy-metal ions.

Polycrystalline metal-organic frameworks Zn4O(1,3,5-benzenetribenzoate)2 (named as MOF-177) with different morphologies have been controlled synthesized through a solvothermal route. MOF-177 electrodes for lithium storage exhibit a relatively high irreversible capacity in the first discharge process and a much lower reversible discharge–charge capacity in the following electrochemical cycles. The performance of MOF-177 is found not impressive for application in reversible lithium storage. A preliminary reaction mechanism on the basis of transmission electron microscopy (TEM) and X-ray photoelectron spectroscopy (XPS) is proposed to understand the chemical behaviors of the electrode.

Aims : To study the anti-tumor effect on MCF-7 cells and the related mechanism of the alcohol extract of Rhodiola algida Var. tangutica Methods : The effective constituent of the extract was detected with the high efficiency liquid chromatography. The survival rate of MCF-7 cells was evaluated by MTT test. The morphological change of cells was observed with optical microscope. The apoptotic influence of the extract on cells was researched with Annexin V-FITC/PI kit and flow cytometry Results : The main constituent of the extract contained the salidroside and tyrosol. The extract caused decrease in the survival of MCF-7. The results of optical microscope showed the vacuole and apoptotic body in MCF-7 cells. The FCM analysis showed that lower concentrations of the extract caused much apoptotic cells and higher concentrations led to much necrotic cells Conclusion : The extract of Rhodiola algida Var. tangutica inhibits division of MCF-7 cells and the cytostatic and antiproliferative effect was related to apoptosis induction mechanism.

Objective To investigate the underlying mechanism of reduced myocardial ischemia-reperfusion (I/R) injury in rats using the traditional Tibetan medicine Sanweitanxiang powder (SWTX). Methods Rats were randomly divided into six groups ( n =10) as follows: (a) propranolol dinitrate control group, given propranolol dinitrate 0.02 g/kg for 10 days before I/R, (b) SWTX with a high dose group, given SWTX 1.5 g/kg for 10 days before I/R, (c) SWTX with a medium dose group, given SWTX 1.25 g/kg for 10 days before I/R, (d) sham group (Sham), in which the rat heart was exposed by pericardiotomy but without I/R, (e) SWTX with a low dose group, given SWTX 1.0 g/kg for 10 days before I/R, and (f) I/R injury group. Rats were intragastrically pretreated with propranolol dinitrate or SWTX. After that, the operation to cause ischemia and reperfusion was conducted. The histopathologic changes of rat hearts were observed by hematoxylin and eosin staining and transmission electron microscopy. Ca 2+ homeostasis protein expression was determined by western blot. Results After SWTX pretreatment, the development of ultrastructural pathological changes from IR injury was attenuated. A decrease in the expression of B-cell lymphoma 2 associated X protein, and an increase in the expression of B-cell lymphoma 2 were observed. An increased activation of extracellular signal regulated kinases were found. Compared with the sham group, the expression of sarcoplasmic reticulum calcium-ATPase, phospholamban, and calsequestrin were all up-regulated after pretreatment with SWTX. Conclusion The protective mechanism of SWTX pretreatment on myocardial I/R injury might be related to its effect on maintaining the balance of calcium homeostasis in rat heart.