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12 results on '"Singh, P.B."'

2. The role of community seed banks in achieving farmers’ rights

Author

Vernooy, Ronnie, Mulesa, Teshome Hunduma, Gupta, Arnab, Jony, Jahangir Alam, Koffi, Kouablan Edmond, Mbozi, Hilton, Singh, P.B., Shrestha, Pitambar, Tjikana, Thabo T., Wakkumbure, C.L.K., Vernooy, Ronnie, Mulesa, Teshome Hunduma, Gupta, Arnab, Jony, Jahangir Alam, Koffi, Kouablan Edmond, Mbozi, Hilton, Singh, P.B., Shrestha, Pitambar, Tjikana, Thabo T., and Wakkumbure, C.L.K.

Abstract

The core objectives of the Convention on Biological Diversity and the International Treaty on Plant Genetic Resources for Food and Agriculture (ITPGRFA) are the conservation and sustainable use of plant genetic resources. The ITPGRFA links these goals explicitly to farmers’ rights. Although farmers’ rights have been debated intensely at international level, their effective implementation at national level remains a major challenge. Community seed banks are good examples of effective implementation of those rights, but have received little attention in scientific literature and policy circles. Case studies in this article from Bangladesh, Côte d’Ivoire, India and Zimbabwe illustrate how this knowledge gap can be filled.

Published
2020

3. Age reprogramming and epigenetic rejuvenation

Author

Singh, P.B. and Newman, A.G.

Abstract

Age reprogramming represents a novel method for generating patient-specific tissues for transplantation. It bypasses the de-differentiation/redifferentiation cycle that is characteristic of the induced pluripotent stem (iPS) and nuclear transfer-embryonic stem (NT-ES) cell technologies that drive current interest in regenerative medicine. Despite the obvious potential of iPS and NT-ES cell-based therapies, there are several problems that must be overcome before these therapies are safe and routine. As an alternative, age reprogramming aims to rejuvenate the specialized functions of an old cell without de-differentiation; age reprogramming does not require developmental reprogramming through an embryonic stage, unlike the iPS and NT-ES cell-based therapies. Tests of age reprogramming have largely focused on one aspect, the epigenome. Epigenetic rejuvenation has been achieved in vitro in the absence of de-differentiation using iPS cell reprogramming factors. Studies on the dynamics of epigenetic age (eAge) reprogramming have demonstrated that the separation of eAge from developmental reprogramming can be explained largely by their different kinetics. Age reprogramming has also been achieved in vivo and shown to increase lifespan in a premature ageing mouse model. We conclude that age and developmental reprogramming can be disentangled and regulated independently in vitro and in vivo.

Published
2018

4. Variation in umami taste perception in the German and Norwegian population

Author

Singh, P.B., Schuster, B., and Seo, H.-S.

Subjects
Monosodium glutamate -- Public opinion, Food/cooking/nutrition, Health
Abstract

In this study, we investigated inter-individual differences in sensitivity to mono-sodium glutamate (MSG) and elucidated the familiarity to umami taste in two European populations. The study consisted of two parts: (1) a survey based on questionnaire and (2) psychophysical screening for inter-individual variation of MSG sensitivity. The psychophysical tests revealed that 3.2% of the German participants and 4.6% of the Norwegian participants were potential non-tasters of MSG. In conclusion, our study confirms inter-individual differences in sensitivity to MSG in humans. European Journal of Clinical Nutrition (2010) 64, 1248-1250; doi: 10.1038/ejcn.2010.133; published online 18 August 2010 Keywords: umami; non-taster; hypotaster; mono-sodium glutamate (MSG); taste, Introduction The specific taste is elicited by L-glutamate and is even more pronounced by monosodium glutamate (MSG; Ikeda, 1909). MSG is naturally present in different palatable foods and is used [...]

Published
2010
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11. Messenger RNA electroporation is highly efficient in mouse embryonic stem cells: successful FLPe- and Cre-mediated recombination.

Author

Ponsaerts, P., Brown, J.P, Van den Plas, D., Van den Eeden, L., Van Bockstaele, D.R, Jorens, P.G, Van Tendeloo, V.F.I, Merregaert, J., Singh, P.B, and Berneman, Z.N

Subjects
MESSENGER RNA, ELECTROPORATION, EMBRYONIC stem cells, LABORATORY mice, TRANSGENES, GENETIC transformation, GENE transfection
Abstract

Development of efficient short-term gene transfer technologies for embryonic stem (ES) cells is urgently needed for various existing and new ES cell-based research strategies. In this study, we present a highly efficient, nonviral non-DNA technology for genetic loading of mouse ES cells based on electroporation of defined mRNA. Here, we show that mouse ES cells can be efficiently loaded with mRNA encoding a green fluorescent reporter protein, resulting in a level of at least 90%of transgene expression without loss of cell viability and phenotype. To show that transgenes, introduced by mRNA electroporation, exert a specific cellular function in transfected cells, we electroporated stably transfected ES cell lines with mRNA encoding FLPe or Cre recombinase proteins in order to excise an FRT- or LoxP-flanked reporter gene. The results, as determined by the disappearance and/or appearance of a fluorescent reporter gene expression, show that FLPe and Cre recombinase proteins, introduced by mRNA electroporation, efficiently exert their function without influence on further culture of undifferentiated ES cell populations and their ability to differentiate towards a specific lineage.Gene Therapy (2004) 11, 1606-1610. doi:10.1038/sj.gt.3302342 Published online 5 August 2004 [ABSTRACT FROM AUTHOR]

Published
2004
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12. Modulatory Actions of MystusGonadotropin on γ-BHC-Induced Histological Changes, Cholesterol, and Sex Steroid Levels in Heteropneustes fossilis

Author

Singh, P.B., Kime, D.E., and Singh, T.P.

Abstract

Female specimens of Heteropneustes fossiliswere exposed to a sublethal concentration of the organochlorine agricultural pesticide γ-BHC (16 mg liter−1)for 4 weeks during the reproductively active preparatory and prespawning phases of the annual reproductive cycle. The effects of γ-BHC on the gonadosomatic index (GSI) and on the histological changes in ovary, cholesterol-free cholesterol (CF), esterified cholesterol (CE), and the sex steroids testosterone-estradiol-17β, and 17α-hydroxyprogesterone in plasma and ovary were monitored. Mystusgonadotropin (mGTH) was administered along with γ-BHC exposure during the preparatory and prespawning phases. γ-BHC decreases GSI during both phases; however, simultaneous administration of mGTH recovered the effect. γ-BHC exposure caused inhibition of ovarian growth, resulting in a large number of stage I oocytes and few stage II oocytes, while at higher doses, mGTH reversed the impact of γ-BHC, and a large number of stage III and vitellogenic oocytes were recorded. This pesticide also inhibited deesterification of CE to CF. Additionally, it impaired ovarian steroidogenesis and secretion of steroids into plasma. Lower doses of mGTH (0.1 and 1.0 μg/fish) were ineffective in modulating the impact of γ-BHC on lipids and steroids. However, higher doses (10 and 20 μg/fish) effectively revoked the impact of γ-BHC, routed through the hypothalamo- hypophyseal-ovarian axis and directly upon the ovary.

Published
1993
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