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Objective To investigate the effect and underlying mechanism of roxadustat on apoptosis and senescence of renal tubular epithelial cell line HK-2 induced by heat stress. Methods After HK-2 cells were treated with different concentrations of roxadustat (10, 20, 30, 40 and 50 μmol/L) for 24 h, CCK-8 assay was used to determine the optimal intervention concentration of roxadustat. HK-2 cells were divided into 4 groups (n=3): control group, roxadustat group (30 μmol/L, 24 h), heat-stress group (43 ℃, 2 h), and heat-stress+roxadustat group (30 μmol/L roxadustat treatmnet for 24 h followed by heat-stress 2 h). Cell viability was detected by CCK-8 assay. Expression of hypoxia-inducible factor-1α (HIF-1α), Cleaved Caspase-3, p16 and p21 at protein level was detected by Western blotting. Immunofluorescence assay was employed to observe the distribution of HIF-1α. β-galactosidase staining kit was utilized to detect SA-β-Gal activity. TUNEL staining was used to measure cell apoptosis. Results The highest cell viability was observed in the cells after 30 μmol/L roxadustat treatment. Heat stress resulted in a significant decrease in cell viability (P < 0.05), elevated protein levels of HIF-1α, Cleaved Caspase-3, p16 and p21(P < 0.05), enhanced SA-β-Gal activity (P < 0.05) and increased percentage of TUNEL-positive cells (P < 0.05) when compared with the cells in the control group. In comparison with the heat-stress group, the heat-stress+roxadustat group showed significant decrease in the protein levels of Cleaved Caspase-3, p16 and p21 (P < 0.05), reduced activity of SA-β-Gal [(65.44±5.00)% vs (77.15±2.61)%, P < 0.05] and decreased percentage of TUNEL-positive cells [(16.73±2.20)% vs (46.40±13.87)%, P < 0.05], but increase in cell viability [(86.33±4.51)% vs (66.33±8.50)%, P < 0.05] as well as HIF-1α protein expression (P < 0.05). Furthermore, immunofluorescence assay showed that HIF-1α was mainly distributed in the nucleus and perinucleus. Conclusion Roxadustat attenuates heat stress-induced apoptosis and senescence of renal tubular epithelial cells by upregulating HIF-1α.

Blasting vibration monitoring and analysis are the basis and premise of the research on the control of blasting vibration damage.Taking the blasting excavation of small spacing tunnel as the engineering background,Matlab was used to decompose the blasting vibration signals by 5 layers of wavelet packet transformation to obtain the wavelet packet frequency band energy of each node.The frequency band energy distribution characteristics of blasting vibration signal was studied,and the attenuation equation of total energy was proposed by regression analysis of total energy.The research shows that the energy of blasting vibration signal is mainly concentrated in the low frequency band(0~300 Hz).The higher the blasting vibration energy is,the faster the attenuation is.At the same time,energy exhibits that high frequency part attenuates faster and the low frequency part attenuates slower.Energy has a tendency toward low frequency with the increase of distance.The energy attenuation coefficient is twice of the peak velocity attenuation coefficient.When the distance from explosion center is in the range 0 to 30 m,energy attenuates rapidly,and energy attenuation tends to be gentle in the range of 30~53.5 m,while energy is almost zero after more than 53.5 m.The research results have important significance for the further study on the dynamic stability of the existing highway tunnels.

To study the ground pressure features of roadway under close range goaf，deformation laws of surrounding rock during mining are analyzed by deformation monitoring.Variations of vertical stress in surrounding rock during mining are obtained by numerical simulation.The results show that the convergence between roof and floor can be 2.02-3.09 times that between two side walls.The intense influence area is 10 m front of working face，where deformation velocity can become fast.The two side walls are influenced more significant in this area.Vertical stress in surrounding rock closed to coal pillar increases when adjacent to the working face.It can be found that the existence of goaf make curves no longer obey exponential function to the base.Working face under goaf has incomplete roof.So effects of abutment pressure decrease.The influence scope of abutment pressure is only 5-10 m，which reduces obviously compared to common roadway.The formation of side abutment pressure in upper coal pillars make a roadway under goaf has a larger deformation，and side walls supports are easy to damage because of a compressive expansion in surrounding rock.Roof sinking caused by the damage of support in side walls，and the seepage from goaf will aggravate the heave of Al-mudstones floor.

As the decision-making brain for power system operation, grid regulation and operation is a comprehensive decision-making control that combines a large amount of data, mechanism analysis, operating procedures and professional experience, and a new generation of artificial intelligence development ideas and evolution characterized by data-driven and knowledge-guided. The directions are very close. However, the current scheduling control is still based on experience and manual analysis. The massive and diverse data of the control center and the lack of logical models between the plans require a large amount of experience and knowledge associations by the control personnel. There are more repetitive human brain labor and relatively low intelligence. Therefore, deep learning is applied to the learning of power control knowledge, and a semantic understanding network based on deep Long Short Term Memory is proposed. It uses sequence labeling to extract in-depth semantic related information of different keywords and query questions, and finds key information about language problems in order to achieve fine-grained and precise query. Experiments show that the proposed network model is superior to the previous methods, and it achieves better performance in the joint extraction of fine-grained evaluation words and evaluation objects, extracts the key information and deep semantic information of query problems and corresponding cases, and realizes power scheduling based on voice interaction The model can be effectively applied in the field of power dispatching and solve a large number of problems in power dispatching and control. [ABSTRACT FROM AUTHOR]

Background: This paper is to describe percutaneous endoscopy in the treatment of lumbar spinal stenosis secondary to ligamentum flavum hypertrophy targeted and to investigate the efficacy and safety of percutaneous endoscopy in the treatment of this kind of lumbar spinal stenosis in elderly patients. Method: A retrospective analysis of 40 elderly patients with lumbar spinal stenosis secondary to ligamentum flavum hypertrophy admitted between January 2016 and January 2018 was performed. According to different surgical methods, they were divided into two groups: the control group and the endoscopy group (interlaminar approach), 20 people per group. There were 9 males and 11 females in the control group; the age of patients was 65.65 ± 4.44 years, and the average disease duration was 4.55 ± 1.85 years. Besides, there were 10 males and 10 females in the endoscopy group; the age of patients was 67.30 ± 4.23 years, and the average disease duration was 4.95 ± 2.04 years. Collect and count surgical-related indicators, preoperative and postoperative radiologic findings, incision visual analog scale (VAS), lumbar and leg pain VAS, lumbar Japanese Orthopaedic Association (JOA), and Oswestry disability index (ODI) scores of all patients. Result: A series of surgical indicators (including the operation time, the quantity of bleeding, and postoperative hospital stay) in the endoscopy group was significantly lower than that in the control group (p < 0.05). The incision VAS score in the endoscopy group was also significantly lower than that in the control group at each time after surgery (p < 0.05). Besides, compared with the control group, in the endoscopy group, the leg pain VAS score and lumbar ODI score after surgery were significantly decreased (p < 0.05). Compared with the control group, in the endoscopy group, the lumbar JOA score was significantly higher (p < 0.05). Conclusion: Percutaneous endoscopic technique is a small trauma, quick recovery, safe, and effective minimally invasive surgery for patients with lumbar spinal stenosis secondary to ligamentum flavum hypertrophy. [ABSTRACT FROM AUTHOR]

Purpose: Colon cancer ranks as the fourth common type of cancer and is responsible for significant morbidity and mortality throughout the world. Late diagnosis and the rarity of potent and safer chemotherapeutic drugs and efficient therapeutic targets create severe obstacle in the treatment of colon cancer. This study was undertaken to examine the anticancer effects of Evodiamine against human colon cancer cells., Methods: The proliferation rate of the SW480 colon cancer cells was monitored by MTT assay. Apoptosis was detected by Annexin V/propidium iodide (PI) and acridine orange (AO)/ethidium bromide (EB) staining. Transmission electron microscopy (TEM) was used for detection of autophagy. Cell migration and invasion was detected by wound healing and transwell assays, respectively. Protein expression was determined by western blotting., Results: Evodiamine suppressed the proliferation of the SW480 colon cancer cells and exhibited an IC50 of 10 µM. The cytotoxic effects of Evodiamine were found to be comparatively lower against the normal CDD-18Co colon cells as evidenced from the IC50 of 100 µM. AO/EB staining showed that Evodiamine caused apoptosis of the SW480 cells and the percentage of the apoptotic SW480 cells increased with increase in the Evodiamine concentration as indicated by annexin V/PI staining. Evodiamine-induced apoptosis was also accompanied by upregulation of caspase-3 and Bax and suppression of Bcl-2. TEM analysis showed that Evodiamine also activated autophagy in the SW480 cells by enhancing the expression of LC3 II and Beclin 1. The wound assay showed that Evodiamine suppressed the migration of the SW480 cells. Evodiamine also reduced the invasion potential of the SW480 cells as suggested by the transwell assay., Conclusion: The findings of the present study suggest that Evodiamine is a potent anticancer agent and may prove beneficial in the development of systemic therapy of colon cancer.

Schwertmannite is an environmental mineral material that can promote the natural passivation of heavy metal elements, thereby reducing environmental pollution from toxic elements. However, the fundamental reason for the difference between the chemically (H₂O₂-FeSO₄) and biologically ( Acidithiobacillus ferrooxidans -FeSO₄) synthesized schwertmannite is still unclear. In this study, X-ray diffraction, scanning electron microscopy, the Brunauer⁻Emmett⁻Teller method, and X-ray fluorescence spectrometry were used to compare the structure, specific surface area, and elemental composition of schwertmannite synthesized by biological and chemical methods. The removal capacity of As(III) by the two kinds of schwertmannite and the effects of extracellular polymeric substances (EPS) on biogenetic schwertmannite were also investigated. At a consistent Fe 2+ oxidation efficiency, the chemical method synthesized more schwertmannite than the biological method over a 60-h period. The biosynthesized schwertmannite had a "chestnut shell" shape, with a larger particle size and specific surface than the chemically synthesized schwertmannite, which was relatively smooth. The saturated adsorption capacities of the biologically and chemically synthesized schwertmannite were 117.0 and 87.0 mg·g -1 , respectively. After exfoliation of the EPS from A. ferrooxidans , the biosynthesized schwertmannite displayed a "wool ball" shape, with rough particle surfaces, many microporous structures, and a larger specific surface area. The schwertmannite yield also increased by about 45% compared with that before exfoliation, suggesting that the secretion of EPS by A. ferrooxidans can inhibit the formation of schwertmannite.

Purpose: Boswellic acid is an important plant-derived natural product with tremendous pharmacological potential and has been reported to inhibit the growth of several types of cancer cells. In this study we report the anticancer activity of boswellic acid against human colon cancer cells via induction of apoptosis, cell cycle arrest and inhibition of cell migration and PI3K/AKT signalling pathway., Methods: The antiproliferative effects of boswellic acid were assessed by MTT assay using different doses of the drug. The apoptotic effects were studied by DAPI and annexin V/PI staining assays, and cell cycle distribution was studied by flow cytometry. The effects of the drug on PI3K/AKT protein expression were studied using western blot analysis., Results: The results of this study showed that boswellic acid suppresses the growth of HCT-116 colon cancer cells. The anticancer effects were found to be dose-dependent and the IC50 value was 15 μM against the HCT-116 colon cancer cells. The inhibition of growth of these cancer cells was mainly due to apoptosis and G2/M cell cycle arrest. Besides, boswellic acid altered the Bax/Bcl-2 ratio in the HCT-116 cancer cells and inhibited their migration as indicated by the cell migration assay. It was observed that boswellic acid decreased the expression of p-PI3K and p-AKT in a concentration- dependent manner. However, the expression of PI3K and AKT remained almost unaltered., Conclusion: In conclusion, these results clearly suggest that boswellic acid could be employed in the treatment of colon cancer provided further in vivo and other in depth experiments are done.