188 results on '"T. Takenouchi"'
Search Results
2. 1218 Diagnostic performance for nail melanoma of melanin degradation products in the nail plate
- Author
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A. Minagawa, T. Omodaka, T. Takenouchi, T. Takai, S. Ito, K. Wakamatsu, and R. Okuyama
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Cell Biology ,Dermatology ,Molecular Biology ,Biochemistry - Published
- 2023
3. Anti-PD1 checkpoint inhibitor therapy in acral melanoma: a multicenter study of 193 Japanese patients
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Y. Nakamura, K. Namikawa, K. Yoshino, S. Yoshikawa, H. Uchi, K. Goto, S. Fukushima, Y. Kiniwa, T. Takenouchi, H. Uhara, T. Kawai, N. Hatta, T. Funakoshi, Y. Teramoto, A. Otsuka, H. Doi, D. Ogata, S. Matsushita, T. Isei, T. Hayashi, Y. Shibayama, and N. Yamazaki
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0301 basic medicine ,Oncology ,medicine.medical_specialty ,Skin Neoplasms ,medicine.medical_treatment ,Programmed Cell Death 1 Receptor ,Pembrolizumab ,03 medical and health sciences ,0302 clinical medicine ,Japan ,Internal medicine ,medicine ,Humans ,Stage (cooking) ,Melanoma ,Retrospective Studies ,business.industry ,Common Terminology Criteria for Adverse Events ,Hematology ,Immunotherapy ,medicine.disease ,Clinical trial ,030104 developmental biology ,030220 oncology & carcinogenesis ,Toxicity ,Nivolumab ,business - Abstract
Acral melanoma (AM) is an epidemiologically and molecularly distinct entity that is underrepresented in clinical trials on immunotherapy in melanoma. We aimed to analyze the efficacy of anti-programmed cell death 1 (anti-PD-1) antibodies in advanced AM.We retrospectively evaluated unresectable stage III or stage IV AM patients treated with an anti-PD-1 antibody in any line at 21 Japanese institutions between 2014 and 2018. The clinicobiologic characteristics, objective response rate (ORR, RECIST), survival estimated using Kaplan-Meier analysis, and toxicity (Common Terminology Criteria for Adverse Events 4.0.) were analyzed to estimate the efficacy of the anti-PD-1 antibodies.In total, 193 patients (nail apparatus, 70; palm and sole, 123) were included in the study. Anti-PD-1 antibody was used as first-line therapy in 143 patients (74.1%). Baseline lactate dehydrogenase (LDH) was within the normal concentration in 102 patients (52.8%). The ORR of all patients was 16.6% (complete response, 3.1%; partial response, 13.5%), and the median overall survival (OS) was 18.1 months. Normal LDH concentrations showed a significantly stronger association with better OS than abnormal concentrations (median OS 24.9 versus 10.7 months; P0.001). Although baseline characteristics were similar between the nail apparatus and the palm and sole groups, ORR was significantly lower in the nail apparatus group [6/70 patients (8.6%) versus 26/123 patients (21.1%); P = 0.026]. Moreover, the median OS in this group was significantly poorer (12.8 versus 22.3 months; P = 0.03).Anti-PD-1 antibodies have limited efficacy in AM patients. Notably, patients with nail apparatus melanoma had poorer response and survival, making nail apparatus melanoma a strong candidate for further research on the efficacy of novel combination therapies with immune checkpoint inhibitors.
- Published
- 2020
4. Laser-excited photoemission spectroscopy study of superconducting boron-doped diamond
- Author
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K. Ishizaka, R. Eguchi, S. Tsuda, T. Kiss, T. Shimojima, T. Yokoya, S. Shin, T. Togashi, S. Watanabe, C.-T. Chen, C.Q. Zhang, Y. Takano, M. Nagao, I. Sakaguchi, T. Takenouchi and H. Kawarada
- Subjects
Materials of engineering and construction. Mechanics of materials ,TA401-492 ,Biotechnology ,TP248.13-248.65 - Abstract
We have investigated the low-energy electronic state of boron-doped diamond thin film by the laser-excited photoemission spectroscopy. A clear Fermi-edge is observed for samples doped above the semiconductor–metal boundary, together with the characteristic structures at 150×n meV possibly due to the strong electron–lattice coupling effect. In addition, for the superconducting sample, we observed a shift of the leading edge below Tc indicative of a superconducting gap opening. We discuss the electron–lattice coupling and the superconductivity in doped diamond.
- Published
- 2006
5. Acoustic and optical phonons in metallic diamond
- Author
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M. Hoesch, T. Fukuda, T. Takenouchi, J.P. Sutter, S. Tsutsui, A.Q.R. Baron, M. Nagao, Y. Takano, H. Kawarada and J. Mizuki
- Subjects
Materials of engineering and construction. Mechanics of materials ,TA401-492 ,Biotechnology ,TP248.13-248.65 - Abstract
The dispersion of acoustic and optical phonons in highly boron-doped diamond has been measured by inelastic X-ray scattering at an energy resolution of 6.4 meV. The sample is doped in the metallic regime and shows superconductivity below 4.2 K (midpoint). The data are compared to pure and nitrogen-doped diamond that represent the non-metallic state. No difference is found for the acoustic phonons in the three samples, while the optical phonons show a shift of the dispersion (softening) in qualitative agreement with earlier results from Raman spectroscopy. The presence of boron and nitrogen incorporated into the diamond lattice leads to structural disorder. Evidence for this is found both in the observation of otherwise symmetry-forbidded Bragg intensity at (0 0 2) and intensity from acoustic phonon modes in the vicinity of (0 0 2).
- Published
- 2006
6. Soft X-ray angle-resolved photoemission spectroscopy of heavily boron-doped superconducting diamond films
- Author
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T. Yokoya, T. Nakamura, T. Matushita, T. Muro, H. Okazaki, M. Arita, K. Shimada, H. Namatame, M. Taniguchi, Y. Takano, M. Nagao, T. Takenouchi, H. Kawarada and T. Oguchi
- Subjects
Materials of engineering and construction. Mechanics of materials ,TA401-492 ,Biotechnology ,TP248.13-248.65 - Abstract
We have performed soft X-ray angle-resolved photoemission spectroscopy (SXARPES) of microwave plasma-assisted chemical vapor deposition diamond films with different B concentrations in order to study the origin of the metallic behavior of superconducting diamond. SXARPES results clearly show valence band dispersions with a bandwidth of ~23 eV and with a top of the valence band at gamma point in the Brillouin zone, which are consistent with the calculated valence band dispersions of pure diamond. Boron concentration-dependent band dispersions near the Fermi level (EF) exhibit a systematic shift of EF, indicating depopulation of electrons due to hole doping. These SXARPES results indicate that diamond bands retain for heavy boron doping and holes in the diamond band are responsible for the metallic states leading to superconductivity at low temperature. A high-resolution photoemission spectroscopy spectrum near EF of a heavily boron-doped diamond superconductor is also presented.
- Published
- 2006
7. 11B-NMR study in boron-doped diamond films
- Author
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H. Mukuda, T. Tsuchida, A. Harada, Y. Kitaoka, T. Takenouchi, Y. Takano, M. Nagao, I. Sakaguchi and H. Kawarada
- Subjects
Materials of engineering and construction. Mechanics of materials ,TA401-492 ,Biotechnology ,TP248.13-248.65 - Abstract
We have investigated an origin of the superconductivity discovered in boron (B)-doped diamonds by means of 11B-NMR on heteroepitaxially grown (1 1 1) and (1 0 0) films and polycrystalline film. The characteristic difference of B-NMR spectral shape for the (1 1 1) and (1 0 0) thin films is demonstrated as arising from the difference in the concentration (nB(1)) of boron substituted for carbon. It is revealed from a scaling between a superconducting transition temperature Tc and nB(1) that the holes doped into diamond via the substitution of boron for carbon are responsible for the onset of superconductivity. The result suggests that the superconductivity in boron-doped diamond is mediated by the electron–phonon interaction brought about a high Debye temperature ~1860 K characteristic for the diamond structure.
- Published
- 2006
8. Recent R&D Activities of Negative-Ion-Based Ion Source for JT-60SA
- Author
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T. Takenouchi, Kazuhiro Watanabe, Yasushi Yamano, L. R. Grisham, K. Oasa, T. Simizu, M. Kawai, A. Honda, S. Kobayashi, Kazuhiro Kobayashi, M. Komata, S. Sasaki, Masaya Hanada, K. Oshima, M. Kamada, Takashi Inoue, Y. Tanai, N. Akino, N. Ebisawa, Yujiro Ikeda, K. Noto, Naotaka Umeda, M. Takahashi, K. Kikuchi, K. Mogaki, M. Kazawa, and K. Usui
- Subjects
Physics ,Nuclear and High Energy Physics ,Tokamak ,Nuclear engineering ,Magnetic confinement fusion ,Condensed Matter Physics ,Grid ,Acceleration voltage ,Ion source ,law.invention ,Beamline ,law ,Active cooling ,Atomic physics ,Voltage - Abstract
The JT-60 Super Advanced (JT-60SA) tokamak aims to perform the ITER support and to demonstrate steady-state high-beta plasma project with the collaboration between Japan and EU. To attain these objectives, the negative-ion-based NBI (N-NBI) system is required to inject 10 MW for 100 s at the beam energy of 500 keV. On JT-60U, the present N-NBI ion source has injected 3.2 MW for 21 s at 320 keV; however, three key issues should be solved for the JT-60SA N-NBI ion source. One is to improve the voltage holding capability of the large negative ion source, where the available acceleration voltage has been limited to less than ~400 kV due to breakdowns. The accelerator of the JT-60U ion source is composed of large three-stage grids and three fiberglass reinforced plastic (FRP) insulators. Recent R&D tests suggested that the FRP insulators were not the main factor to trigger the breakdowns at the early conditioning stage. The accelerator with a large area of grids and their supporting structure may need a high margin in the design of electric field and a long time for conditioning. The second issue is to reduce the power loading of the acceleration grids. It was found that some beamlets were strongly deflected due to beamlet-beamlet interaction and strike on the grounded grid in the accelerator. Moreover, the electrons generated in the accelerator caused the grid loading and the overheating of the beamline components. The acceleration grids for JT-60SA are to be designed by taking account of the beamlet-beamlet interaction and the applied magnetic field in 3-D simulation. Third is to maintain the D production for 100 s. Although a constant D- beam power was confirmed on JT-60U for 21 s, an active cooling system is required to keep the temperature of the plasma grid (PG) under optimum condition during 100-s operation. A simple cooling structure is proposed for the active cooled PG, where a key is the temperature gradient on the PG for uniform D- production. In the present schedule, design work, reflecting the latest R&D progress, will continue until ~2011. The modified N-NBI ion source will start on JT-60SA in 2015.
- Published
- 2008
9. Application of PLC to dynamic control system for liquid He cryogenic pumping facility on JT-60U NBI system
- Author
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T. Takenouchi, Katsumi Ooshima, Fuminori Okano, K. Kikuchi, S. Numazawa, N. Akino, Yujiro Ikeda, Y. Tanai, and A. Honda
- Subjects
Function block diagram ,Computer science ,Mechanical Engineering ,Simatic S5 PLC ,Programmable logic controller ,ComputerApplications_COMPUTERSINOTHERSYSTEMS ,Control engineering ,Data acquisition ,Nuclear Energy and Engineering ,SCADA ,Control system ,General Materials Science ,Direct digital control ,Distributed control system ,Civil and Structural Engineering - Abstract
The control system of the cryogenic facility in the JT-60 NBI system has been replaced by employing the PLC (Programmable Logic Controller) and SCADA (Supervisory Control And Data Acquisition) system. The original control system was constructed about 20 years ago by specifying the DCS (Distributed Control System) computer to deal with ∼400 feedback loops. Recently, troubles on this control system have increased due to its age-induced deterioration. To maintain the high reliability of the cryogenic facility, a new control system has been planned with the PLC and SCADA systems. Their attractive features include high market availability and cost-effectiveness, however, the use of PLC for such a large facility with ∼400 feedback loops has not been established because of insufficient processing capability of the early PLC. Meanwhile, the recent progress in the PLC enables to use the FBD (function block diagram) programming language for 500 function blocks. By optimizing the function blocks and connecting them in the FBD language, the feedback loops have been successfully replaced from DCS to PLC without a software developer. Moreover, an oscillation of the liquid He level, which often occurs during the cooldown mode of the cryopumps, can be automatically stabilized by easily adding a new process program in the PLC. At present, the new control system has worked well.
- Published
- 2008
10. Technical design of NBI system for JT-60SA
- Author
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M. Kawai, Makoto Matsukawa, K. Kikuchi, M. Komata, M. Kazawa, Fuminori Okano, M. Kamada, T. Takenouchi, Y. Tanai, N. Ebisawa, N. Akino, Mitsuru Kikuchi, Naotaka Umeda, Hiroshi Tamai, Yujiro Ikeda, K. Oshima, K. Noto, A. Honda, T. Ohga, Kazuhiro Watanabe, Masaya Hanada, Takashi Inoue, K. Mogaki, K. Usui, and H. Yamazaki
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Tokamak ,Materials science ,Mechanical Engineering ,Nuclear engineering ,Time constant ,Fusion power ,Ion source ,Technical design ,Power (physics) ,law.invention ,Pulse (physics) ,Nuclear Energy and Engineering ,law ,Active cooling ,General Materials Science ,Civil and Structural Engineering - Abstract
Modification of JT-60U to a superconducting device (so called JT-60SA) has been planned to contribute to ITER and DEMO. The NBI system is required to inject D 0 beams of 34 MW for 100 s. The upgraded NBI system for JT-60SA consists of 12 positive-ion-based NBI (P-NBI) units and one negative-ion-based NBI (N-NBI) unit. The injection powers of each P-NBI and N-NBI units are 2 MW at 85 keV and 10 MW at 500 keV, respectively. On JT-60U, the long pulse operations of 30 s at 2 MW and 20 s at 3.2 MW have been achieved on the P-NBI and N-NBI units, respectively. Both units have demonstrated no injection power degradation for long pulse operation. It has been also found that the thermal time constants of the main key components with active cooling, such as the ion source of the present NBI system, are less than ∼20 s. Therefore, the pulse extension up to 100 s is expected to need some modifications mainly the power supply system. In addition, the voltage-holding capability of the negative ion source is required to be improved. The detailed technical design of the NBI system for JT-60SA is presented.
- Published
- 2007
11. Superconducting properties of homoepitaxial CVD diamond
- Author
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T. Okutsu, Hiroshi Kawarada, T. Takenouchi, Minoru Tachiki, Hitoshi Umezawa, S. Ueda, Yoshihiko Takano, Satoshi Ishii, and Isao Sakaguchi
- Subjects
inorganic chemicals ,Superconductivity ,Materials science ,Synthetic diamond ,Mechanical Engineering ,Transition temperature ,Analytical chemistry ,Diamond ,chemistry.chemical_element ,General Chemistry ,Chemical vapor deposition ,engineering.material ,Electronic, Optical and Magnetic Materials ,law.invention ,Carbon film ,chemistry ,law ,Plasma-enhanced chemical vapor deposition ,Materials Chemistry ,engineering ,Electrical and Electronic Engineering ,Boron - Abstract
Superconductivity was achieved above 10 K in heavily boron-doped diamond thin films deposited by the microwave plasma-assisted chemical vapor deposition (CVD) method. Advantages of the CVD method are the controllability of boron concentration in a wide range, and a high boron concentration, compared to those obtained using the high-pressure high-temperature method. The superconducting transition temperatures of homoepitaxial (111) films are determined to be 11.4 K for TC onset and 8.4 K for zero resistance from transport measurements. In contrast, the superconducting transition temperatures of (100) films TC onset = 6.3 K and TC zero = 3.2 K were significantly suppressed.
- Published
- 2007
12. The effects of FasL on inflammation and tumor survival are dependent on its expression levels
- Author
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Masatoshi Tagawa, Akihiko Wada, Yuji Tada, Takayuki Kuriyama, K. Tatsumi, Jiyang O-Wang, T Takenouchi, Kiyoko Kawamura, and Yasuo Takiguchi
- Subjects
Cytotoxicity, Immunologic ,Cancer Research ,Fas Ligand Protein ,Neutrophils ,T-Lymphocytes ,CD40 Ligand ,Melanoma, Experimental ,Mice, Nude ,Apoptosis ,chemical and pharmacologic phenomena ,Inflammation ,Transfection ,Fas ligand ,Carcinoma, Lewis Lung ,Mice ,Immune system ,In vivo ,Immunity ,In Situ Nick-End Labeling ,medicine ,Animals ,Humans ,Molecular Biology ,Mice, Inbred BALB C ,business.industry ,Lewis lung carcinoma ,hemic and immune systems ,Flow Cytometry ,Coculture Techniques ,Mice, Inbred C57BL ,Immunology ,Molecular Medicine ,Female ,Ectopic expression ,medicine.symptom ,business ,Spleen - Abstract
The apoptosis-inducing Fas ligand (FasL) is expressed in a variety of human cancers and has been implicated in tumor immune evasion. Paradoxically, ectopic expression of FasL in experimental tumors triggers a neutrophil-mediated inflammatory response and tumor rejection. To resolve these conflicting findings, we have established B16 melanoma and P29 Lewis lung carcinoma lines expressing different levels of FasL and examined their tumorigenicity in vivo. While tumors with a high level of FasL were rapidly rejected as previously reported, those expressing a low level of FasL were not rejected but grew faster than did FasL-negative parental cells. The growth enhancement of FasL(low) tumors was not observed in T-cell-deficient nude mice, suggesting that FasL expressed in tumors at low levels counteracted against T-cell-dependent antitumor responses. In support of this notion, FasL(low) tumors were found to grow faster than parental cells in mice that had acquired tumor-specific immunity. Furthermore, histological examinations revealed apoptosis of lymphocytes in tissue sections of FasL(low) tumors. These results collectively suggest that FasL on tumors is a double-edged sword: at high levels it triggers tumor rejection whereas at low levels it facilitates tumor growth possibly by suppressing antitumor immune responses.
- Published
- 2006
13. Scanning tunneling microscopy and spectroscopy studies of superconducting boron-doped diamond films
- Author
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Takayuki Kiss, Chuanguo Zhang, Isao Sakaguchi, Caiyun Chen, Syunsuke Tsuda, Kyoko Ishizaka, Shuntaro Watanabe, Yoshihiko Takano, Shik Shin, Hiroshi Kawarada, Takahiro Shimojima, Ritsuko Eguchi, Tadashi Togashi, Masanori Nagao, Takayoshi Yokoya, and T. Takenouchi
- Subjects
Superconductivity ,Materials science ,Condensed matter physics ,Photoemission spectroscopy ,Material properties of diamond ,Inverse photoemission spectroscopy ,Doping ,Analytical chemistry ,Diamond ,Angle-resolved photoemission spectroscopy ,02 engineering and technology ,engineering.material ,010402 general chemistry ,021001 nanoscience & nanotechnology ,01 natural sciences ,0104 chemical sciences ,Condensed Matter::Materials Science ,Condensed Matter::Superconductivity ,Excited state ,engineering ,Condensed Matter::Strongly Correlated Electrons ,General Materials Science ,0210 nano-technology - Abstract
We have investigated the low-energy electronic state of boron-doped diamond thin film by the laser-excited photoemission spectroscopy. A clear Fermi-edge is observed for samples doped above the semiconductor–metal boundary, together with the characteristic structures at 150 � n meV possibly due to the strong electron–lattice coupling effect. In addition, for the superconducting sample, we observed a shift of the leading edge below T c indicative of a superconducting gap opening. We discuss the electron–lattice coupling and the superconductivity in doped diamond. r 2006 NIMS and Elsevier Ltd. All rights reserved.
- Published
- 2006
14. Superconductivity and low temperature electrical transport in B-doped CVD nanocrystalline diamond
- Author
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Masanori Nagao, Alfred Q. R. Baron, Jun'ichiro Mizuki, Satoshi Tsutsui, Yoshihiko Takano, M. Hoesch, T. Takenouchi, Tatsuo Fukuda, J. P. Sutter, and Hiroshi Kawarada
- Subjects
Superconductivity ,Materials science ,Condensed matter physics ,Phonon ,Scattering ,Material properties of diamond ,Diamond ,engineering.material ,Condensed Matter::Materials Science ,symbols.namesake ,Condensed Matter::Superconductivity ,Dispersion (optics) ,symbols ,engineering ,Condensed Matter::Strongly Correlated Electrons ,General Materials Science ,Diamond cubic ,Raman spectroscopy - Abstract
The dispersion of acoustic and optical phonons in highly boron-doped diamond has been measured by inelastic X-ray scattering at an energy resolution of 6.4 meV. The sample is doped in the metallic regime and shows superconductivity below 4.2 K (midpoint). The data are compared to pure and nitrogen-doped diamond that represent the non-metallic state. No difference is found for the acoustic phonons in the three samples, while the optical phonons show a shift of the dispersion (softening) in qualitative agreement with earlier results from Raman spectroscopy. The presence of boron and nitrogen incorporated into the diamond lattice leads to structural disorder. Evidence for this is found both in the observation of otherwise symmetry-forbidded Bragg intensity at (0 0 2) and intensity from acoustic phonon modes in the vicinity of (0 0 2).
- Published
- 2006
15. Stereophotographs of diamond and graphite
- Author
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T. Takenouchi, Isao Sakaguchi, H. Mukuda, Akira Harada, Masanori Nagao, Y. Kitaoka, T. Tsuchida, Yoshihiko Takano, and Hiroshi Kawarada
- Subjects
Superconductivity ,Materials science ,Condensed matter physics ,Material properties of diamond ,Doping ,Analytical chemistry ,chemistry.chemical_element ,Diamond ,engineering.material ,symbols.namesake ,chemistry ,symbols ,engineering ,General Materials Science ,Diamond cubic ,Boron ,Carbon ,Debye model - Abstract
We have investigated an origin of the superconductivity discovered in boron (B)-doped diamonds by means of 11B-NMR on heteroepitaxially grown (111) and (100) films and polycrystalline film. The characteristic difference of B-NMR spectral shape for the (111Þ and (100) thin films is demonstrated as arising from the difference in the concentration (nB(1)) of boron substituted for carbon. It is revealed from a scaling between a superconducting transition temperature Tc and nB(1) that the holes doped into diamond via the substitution of boron for carbon ar responsible for the onset of superconductivity. The result suggests that the superconductivity in boron-doped diamond is mediated by the electron–phonon interaction brought about a high Debye temperature ˜ 1860 K characteristic for the diamond structure.
- Published
- 2006
16. Development of High Resolution TOF Detector for RI Beams Using Cherenkov Radiation
- Author
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A. Mizukami, A. Kitagawa, M. Tanaka, K. Abe, Toshio Suzuki, Takuji Izumikawa, Y. Kanke, Mitsunori Fukuda, J. Muraoka, K. Aoki, Takashi Ohtsubo, Ryosuke Kanbe, I. Kato, N. Tadano, Mototsugu Mihara, S. Ohmika, S. Yagi, S. Fukuda, Y. Takeuchi, Shinji Suzuki, E. Miyata, Yukichi Tanaka, M. Takechi, S. Yamaoka, Shinji Sato, H. Takahashi, K. Chikaato, T. Nishimura, K. Ohnishi, Takayuki Yamaguchi, Y. Takei, D. Nishimura, K. Yokoyama, H. Shimamura, H. Du, H. Kikuchi, R. Takagaki, T. Takenouchi, H. Oikawa, Jiro Shimaya, J. Nagumo, A. Ikeda, N. Kanda, M. Machida, and K. Nishizuka
- Subjects
Physics ,Optics ,Cherenkov detector ,law ,business.industry ,Detector ,General Physics and Astronomy ,High resolution ,business ,Cherenkov radiation ,law.invention - Published
- 2017
17. Two cases of vulval pigmented extramammary Paget’s disease: histochemical and immunohistochemical studies
- Author
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S. Nomoto, Satoshi Yamada, H. Chiba, T. Takenouchi, Masahiro Ito, O. Tago, and T. Kazama
- Subjects
Male ,Pathology ,medicine.medical_specialty ,Dermatology ,Melanocyte ,Extramammary Paget's disease ,Stain ,Vulva ,Melanin ,medicine ,Humans ,Aged ,Vulvar Neoplasms ,Epidermis (botany) ,business.industry ,Middle Aged ,medicine.disease ,Immunohistochemistry ,HMB-45 ,Paget Disease, Extramammary ,medicine.anatomical_structure ,Melanocytes ,Female ,business ,Pigmentation Disorders - Abstract
We describe two Japanese female patients with pigmented extramammary Paget’s disease (EMPD); one patient had a dark brown plaque and the other had a reddish patch with a pigmented area, both affecting the vulval region. Histochemical and immunohistochemical examinations confirmed EMPD with melanocyte colonization; plump tumour cells with a large nucleus and pale cytoplasm that were positive for CAM 5.2 and CEA proliferated singly or in nests in the epidermis, and scattered among the tumour cells were many dendritic cells with a large amount of melanin that were positive for HMB-45 and S-100 protein. Fontana–Masson (FM) stain showed many positive cells with well-developed dendritic processes within and around tumour nests. Histochemical and immunohistochemical studies of non-pigmented EMPD cases on the same region showed that HMB-45 positive cells were sparse or not detected at all, and that also FM staining-positive cells were decreased or not detected, and their dendritic processes were poorly formed. The present study suggests that there might be heterogeneity in EMPD in terms of relationships between Paget’s cells and melanocytes.
- Published
- 2000
18. Beamline performance of 500 keV negative ion-based NBI system for JT-60U
- Author
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H. Oohara, N. Ebisawa, A. Honda, R. Toyokawa, N. Isozaki, F. Satoh, T. Ohga, K. Mogaki, Yamamoto Masahiro, T. Itoh, Masaaki Kuriyama, Kazuhiro Watanabe, K. Usui, M. Kazawa, Mikito Kawai, Yoshihiro Ohara, Tetsuo Aoyagi, T. Takenouchi, J. Koizumi, N. Akino, Yoshikazu Okumura, T. Yamazaki, K. Ooshima, Takashi Inoue, and H. Seki
- Subjects
Tokamak ,Materials science ,Ion beam ,Mechanical Engineering ,Plasma ,Neutral beam injection ,law.invention ,Magnetic field ,Ion ,Nuclear Energy and Engineering ,Beamline ,law ,General Materials Science ,Atomic physics ,Beam (structure) ,Civil and Structural Engineering - Abstract
Construction of the 500 keV negative-ion-based NBI system (N-NBI) with its maximum capability of 10 MW and 10 s for NB current drive and plasma core heating in high-density plasmas in JT-60 was completed in March 1996. The neutral beam injection into JT-60 with N-NBI has been performed since then. One of the key issues in the injection was how to control the bending magnetic field for residual ion beams. In N-NBI for JT-60, a stray magnetic field from the tokamak was actively used for bending of the residual ion beams. Combination of the stray magnetic field from JT-60 and the magnetic field of the deflecting coils was controlled sufficiently to successfully deposit residual ion beams at a constant point on the ion dump surface. The deposited positive and negative beam profiles on the ion dump were measured with an array of thermocouples brazed on the dump surface. This report describes the performance test of bending the residual ion beams and the measurement of the heat load on the ion dump for JT-60 N-NBI experiments.
- Published
- 1998
19. Operation of the negative-ion based NBI for JT-60U
- Author
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Yamamoto Masahiro, T. Itoh, A. Honda, N. Isozaki, T. Takenouchi, N. Ebisawa, F. Satoh, K. Mogaki, M. Kazawa, Mikito Kawai, T. Ohga, H. Oohara, K. Ohshima, Yoshihiro Ohara, Kazuhiro Watanabe, N. Akino, Y. Toyokawa, K. Usui, J. Koizumi, Tetsuo Aoyagi, Yoshikazu Okumura, T. Yamazaki, Takashi Inoue, C. Zhou, and Masaaki Kuriyama
- Subjects
Materials science ,Hydrogen ,Mechanical Engineering ,chemistry.chemical_element ,Plasma ,Ion source ,Neutral beam injection ,Ion ,Nuclear Energy and Engineering ,Deuterium ,chemistry ,Physics::Plasma Physics ,Physics::Accelerator Physics ,General Materials Science ,Atomic physics ,Beam energy ,Beam (structure) ,Civil and Structural Engineering - Abstract
A beam injection experiment with the negative-ion based NBI system (N-NBI) started in March 1996 on JT-60U. After achieving the first neutral beam injection of 180 keV, ∼0.1 MW for 0.4 s into the JT-60U plasmas, the operation parameters of the ion source and power supply had been optimized for increasing the beam energy and beam current. In September 1996, a deuterium neutral beam of 2.5 MW at 350 keV was injected into JT-60U using two ion sources. In the operation with hydrogen at the beginning of 1997, a negative ion beam current of 18.4 A at 350 keV has been obtained, and a neutral beam of 3.2 MW at 350 keV for 1 s has been injected into the plasma with one ion source. A neutralization efficiency of negative ion beam has been confirmed to be about 60% at the beam energies of 250–385 keV as predicted theoretically.
- Published
- 1998
20. Hydrophilicity of quinolones is not an exclusive factor for decreased activity in efflux-mediated resistant mutants of Staphylococcus aureus
- Author
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Y Iwata, S Ohya, M Sugawara, H Hanzawa, F Tabata, and T Takenouchi
- Subjects
DNA Topoisomerase IV ,Carbonyl Cyanide m-Chlorophenyl Hydrazone ,Staphylococcus aureus ,Protonophore ,Microbial Sensitivity Tests ,Quinolones ,Biology ,medicine.disease_cause ,Carbonyl cyanide m-chlorophenyl hydrazone ,DNA gyrase ,Piperazines ,Microbiology ,Structure-Activity Relationship ,chemistry.chemical_compound ,Anti-Infective Agents ,Bacterial Proteins ,Escherichia coli ,medicine ,Point Mutation ,Pharmacology (medical) ,Norfloxacin ,Antibacterial agent ,Pharmacology ,Drug Resistance, Microbial ,DNA Topoisomerases, Type II ,Infectious Diseases ,chemistry ,DNA Gyrase ,Efflux ,Multidrug Resistance-Associated Proteins ,Fluoroquinolones ,Research Article ,medicine.drug - Abstract
The elevated expression of the norA gene is responsible for efflux-mediated resistance to quinolones in Staphylococcus aureus (E.Y.W. Ng, M. Trucksis, and D.C. Hooper, Antimicrob. Agents Chemother. 38:1345-1355, 1994). For S. aureus transformed with a plasmid containing the cloned norA gene, SA113(pTUS20) (H. Yoshida, M. Bogaki, S. Nakamura, K. Ubukata, and M. Konno, J. Bacteriol. 172:6942-6949, 1990), and an overexpressed mutant, SA-1199B (G.W. Kaatz, S.M. Seo, and C.A. Ruble, J. Infect. Dis. 163:1080-1086, 1991), the MICs of norfloxacin increased 16 and 64 times compared with its MICs for the recipient and wild-type strains, SA113 and SA-1199, respectively. MICs of CS-940, however, increased only two and eight times, even though these two fluoroquinolones are similarly hydrophilic (apparent logPs of approximately -1). No good correlation was found, among 15 developed and developing quinolones, between the increment ratio in MICs and hydrophobicity (r = 0.61). Analysis of the quantitative structure-activity relationship among 40 fluoroquinolones revealed that the MIC increment ratio was significantly correlated with the bulkiness of the C-7 substituent and bulkiness and hydrophobicity of the C-8 substituent of fluoroquinolones (r = 0.87) and not with its molecular hydrophobicity (r = 0.47). Cellular accumulation of norfloxacin in SA-1199B was significantly lower than that in SA-1199, and it was increased by addition of carbonyl cyanide m-chlorophenyl hydrazone. On the other hand, accumulations of CS-940 in these strains were nearly identical, and they were not affected by addition of the protonophore.
- Published
- 1996
21. Development of Negative-ion Based NBI System for JT-60
- Author
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Shuhichi Kawashima, Mamoru Matsuoka, T. Ohga, Naoki Miyamoto, Yoshihiro Ohara, Takashi Inoue, Y. Fujiwara, Shunji Takahashi, Yamamoto Masahiro, Junnichi Koizumi, Yoshikazu Okumura, Hirotugu Usami, K. Usui, Atushi Honda, T. Itoh, Noboru Ebisawac, H. Oohara, Tetsuo Aoyagi, Yohichi Ono, K. Miyamoto, K. Mogaki, T. Yamazaki, N. Akino, T. Takenouchi, Katumi Ohshima, M. Kazawa, Mikito Kawai, Nobumitu Isozaki, Masaaki Kuriyama, Hujio Satoh, Kazuhiro Watanabe, and Masahiro Tanii
- Subjects
Nuclear physics ,Physics ,Nuclear Energy and Engineering ,JT-60 ,Ion - Published
- 1996
22. A subnanosecond clock cryogenic system for Josephson computers
- Author
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Atsuki Inoue, T. Takenouchi, H. Suzuki, K. Fukase, S. Yoshida, Shinya Hasuo, Y. Kamioka, Seigo Kotani, F. Miyagawa, and T. Sano
- Subjects
Materials science ,business.industry ,Cryogenic system ,Electrical engineering ,Refrigerator car ,Cryogenics ,Condensed Matter Physics ,Electronic, Optical and Magnetic Materials ,law.invention ,Printed circuit board ,Microprocessor ,Semiconductor ,law ,Condensed Matter::Superconductivity ,Electrical and Electronic Engineering ,business - Abstract
Using Josephson chips maintained at 4.4 K by a closed-cycle 3-W desk-size refrigerator, the authors verified the correct operation of a 4-b Josephson microprocessor up to 1.1 GHz. Room temperature and liquid He temperature regions are connected with a 130-ps delay using I/O cables passing through sidewalls of a vacuum vessel. It is proposed that both semiconductor and Josephson devices can coexist on circuit boards that constitute the equivalent of a single board. >
- Published
- 1991
23. Phonon softening in superconducting diamond
- Author
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J. P. Sutter, T. Takenouchi, Yoshihiko Takano, Masanori Nagao, Jun'ichiro Mizuki, Moritz Hoesch, Hiroshi Kawarada, Satoshi Tsutsui, Tatsuo Fukuda, and Alfred Q. R. Baron
- Subjects
Superconductivity ,Materials science ,Condensed matter physics ,Scattering ,Phonon ,Diamond ,engineering.material ,Condensed Matter Physics ,Electronic, Optical and Magnetic Materials ,Brillouin zone ,Condensed Matter::Materials Science ,Coupling parameter ,Condensed Matter::Superconductivity ,Pairing ,engineering ,Condensed Matter::Strongly Correlated Electrons ,Softening - Abstract
We observe strong softening of optical phonon modes in superconducting (Tc = 4.2 K) borondoped diamond near the Brillouin zone center using inelastic x-ray scattering from a CVD-grown highly oriented sample. The magnitude of the softening, and our observation that it becomes stronger approaching zone center, supports theoretical models suggesting a phonon-mediated pairing mechanism via coupling of optical phonon modes to Fermi surfaces around the zone center. The electron-phonon coupling parameter is determined as approximately λ = 0.33.
- Published
- 2007
24. Microscopic evidence for evolution of superconductivity by effective carrier doping in boron-doped diamond:B11−NMRstudy
- Author
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Yoshio Kitaoka, Hiroshi Kawarada, Isao Sakaguchi, Masanori Nagao, Tamio Oguchi, Akira Harada, T. Tsuchida, Hidekazu Mukuda, Yoshihiko Takano, and T. Takenouchi
- Subjects
Superconductivity ,Boron doped diamond ,Materials science ,Hydrogen ,Condensed matter physics ,Doping ,chemistry.chemical_element ,Charge (physics) ,Condensed Matter Physics ,Spectral line ,Electronic, Optical and Magnetic Materials ,Condensed Matter::Materials Science ,chemistry ,Condensed Matter::Superconductivity ,Density of states ,Boron - Abstract
We have investigated the superconductivity discovered in boron-doped diamonds by means of $^{11}\mathrm{B}\text{\ensuremath{-}}\mathrm{NMR}$ on heteroepitaxially grown (111) and (100) films. $^{11}\mathrm{B}\text{\ensuremath{-}}\mathrm{NMR}$ spectra for all of the films are identified to arise from the substitutional B(1) site as single occupation and lower symmetric B(2) site substituted as $\text{boron}+\text{hydrogen}$ $(\mathrm{B}+\mathrm{H})$ complex, respectively. Clear evidence is presented that the effective carriers introduced by B(1) substitution are responsible for the superconductivity, whereas the charge neutral B(2) sites does not offer the carriers effectively. The result is also corroborated by the density of states deduced by $1∕{T}_{1}T$ measurement, indicating that the evolution of superconductivity is driven by the effective carrier introduced by substitution at B(1) site.
- Published
- 2007
25. Low-Energy Electrodynamics of Superconducting Diamond
- Author
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Masanori Nagao, Stefano Lupi, Ulrich Schade, Leonetta Baldassarre, Hiroshi Kawarada, Paolo Calvani, T. Takenouchi, Yoshihiko Takano, and Michele Ortolani
- Subjects
Superconductivity ,Physics ,Range (particle radiation) ,Condensed matter physics ,Condensed Matter - Superconductivity ,FOS: Physical sciences ,General Physics and Astronomy ,Synchrotron radiation ,Diamond ,engineering.material ,Lambda ,Omega ,novel superconductors ,Superconductivity (cond-mat.supr-con) ,Condensed Matter::Materials Science ,THIN-FILMS ,Low energy ,Condensed Matter::Superconductivity ,engineering ,Sum rule in quantum mechanics ,TEMPERATURE - Abstract
Heavily-boron-doped diamond films become superconducting with critical temperatures $T_c$ well above 4 K. Here we first measure the reflectivity of such a film down to 5 cm$^{-1}$, by also using Coherent Synchrotron Radiation. We thus determine the optical gap, the field penetration depth, the range of action of the Ferrell-Glover-Tinkham sum rule, and the electron-phonon spectral function. We conclude that diamond behaves as a dirty BCS superconductor., Comment: 4 pages including 3 figures
- Published
- 2006
26. Observation of superconducting gap in boron-doped diamond by laser-excited photoemission spectroscopy
- Author
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Isao Sakaguchi, Ritsuko Eguchi, Shunsuke Tsuda, C. T. Chen, Takayoshi Yokoya, Takahiro Shimojima, Tadashi Togashi, Hiroshi Kawarada, Masanori Nagao, Yoshihiko Takano, Shuntaro Watanabe, Kyoko Ishizaka, Takayuki Kiss, Chuanguo Zhang, T. Takenouchi, Shik Shin, and Ashish Chainani
- Subjects
Superconductivity ,Materials science ,Condensed matter physics ,Photoemission spectroscopy ,Condensed Matter - Superconductivity ,Inverse photoemission spectroscopy ,General Physics and Astronomy ,FOS: Physical sciences ,Angle-resolved photoemission spectroscopy ,Electronic structure ,Laser ,law.invention ,Superconductivity (cond-mat.supr-con) ,law ,Excited state ,Condensed Matter::Superconductivity ,Quasiparticle - Abstract
We have investigated the low energy electronic state of a boron-doped diamond thin film by the ultrahigh resolution laser-excited photoemission spectroscopy. We observed a clear shift of the leading edge below 11 K indicative of a superconducting gap opening ($\Delta \sim 0.78$ meV at 4.5 K). The gap feature is significantly broad and the well-defined quasiparticle peak is not recognizable even at the lowest temperature of measurement 4.5 K. We discuss our result in terms of possible disorder effect on superconductivity in this system., Comment: 12 pages, 4 figures
- Published
- 2006
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27. Origin of the metallic properties of heavily boron-doped superconducting diamond
- Author
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T. Takenouchi, Takayuki Muro, Tetsuya Nakamura, Tamio Oguchi, Tomohiro Matsushita, Hiroshi Kawarada, Takayoshi Yokoya, Yoshihiko Takano, and Masanori Nagao
- Subjects
Superconductivity ,Multidisciplinary ,Materials science ,Condensed matter physics ,business.industry ,Material properties of diamond ,Doping ,Diamond ,Electronic structure ,engineering.material ,Condensed Matter::Materials Science ,Semiconductor ,Condensed Matter::Superconductivity ,engineering ,Condensed Matter::Strongly Correlated Electrons ,Charge carrier ,business ,Electronic band structure - Abstract
The recent discovery that heavily boron-doped diamond is a superconductor with a transition temperature of 7.4 K raises the prospect of superconducting devices with the unique properties of diamond. A study of the electronic structure responsible for superconductivity in heavily boron-doped diamond supports the idea that superconductivity is phonon-mediated, and provides information on the electronic structure that must be retained in order to harness this effect in practical devices. The physical properties of lightly doped semiconductors are well described by electronic band-structure calculations and impurity energy levels1. Such properties form the basis of present-day semiconductor technology. If the doping concentration n exceeds a critical value nc, the system passes through an insulator-to-metal transition and exhibits metallic behaviour; this is widely accepted to occur as a consequence of the impurity levels merging to form energy bands2. However, the electronic structure of semiconductors doped beyond nc have not been explored in detail. Therefore, the recent observation of superconductivity emerging near the insulator-to-metal transition3 in heavily boron-doped diamond4,5 has stimulated a discussion on the fundamental origin of the metallic states responsible for the superconductivity. Two approaches have been adopted for describing this metallic state: the introduction of charge carriers into either the impurity bands6 or the intrinsic diamond bands7,8,9. Here we show experimentally that the doping-dependent occupied electronic structures are consistent with the diamond bands, indicating that holes in the diamond bands play an essential part in determining the metallic nature of the heavily boron-doped diamond superconductor. This supports the diamond band approach and related predictions, including the possibility of achieving dopant-induced superconductivity in silicon and germanium7. It should also provide a foundation for the possible development of diamond-based devices10.
- Published
- 2005
28. In vitro and in vivo antibacterial activities of CS-834, a novel oral carbapenem
- Author
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T Fukuoka, S Ohya, Y Utsui, H Domon, T Takenouchi, T Koga, N Masuda, H Kawada, M Kakuta, M Kubota, C Ishii, E Sakagawa, T Harasaki, A Hirasawa, T Abe, H Yasuda, M Iwata, and S Kuwahara
- Subjects
Imipenem ,Carbapenem ,Administration, Oral ,Microbial Sensitivity Tests ,Biology ,Muramoylpentapeptide Carboxypeptidase ,Cefpodoxime ,beta-Lactamases ,Microbiology ,Cefmenoxime ,Mice ,Cefteram ,Bacterial Proteins ,Drug Stability ,Pneumonia, Staphylococcal ,medicine ,Animals ,Penicillin-Binding Proteins ,Pharmacology (medical) ,Prodrugs ,Antibacterial agent ,Pharmacology ,Ceftizoxime ,Bacterial Infections ,Cefdinir ,Cephalosporins ,Infectious Diseases ,Carbapenems ,Hexosyltransferases ,Peptidyl Transferases ,Thienamycins ,Cefditoren Pivoxil ,Carrier Proteins ,Cefditoren ,medicine.drug ,Research Article - Abstract
CS-834 is a novel oral carbapenem antibiotic. This compound is an ester-type prodrug of the active metabolite R-95867. The antibacterial activity of R-95867 was tested against 1,323 clinical isolates of 35 species and was compared with those of oral cephems, i.e., cefteram, cefpodoxime, cefdinir, and cefditoren, and that of a parenteral carbapenem, imipenem. R-95867 exhibited a broad spectrum of activity covering both gram-positive and -negative aerobes and anaerobes. Its activity was superior to those of the other compounds tested against most of the bacterial species tested. R-95867 showed potent antibacterial activity against clinically significant pathogens: methicillin-susceptible Staphylococcus aureus including ofloxacin-resistant strains, Streptococcus pneumoniae including penicillin-resistant strains, Clostridium perfringens, Neisseria spp., Moraxella catarrhalis, most members of the family Enterobacteriaceae, and Haemophilus influenzae (MIC at which 90% of strains are inhibited, < or =0.006 to 0.78 microg/ml). R-95867 was quite stable to hydrolysis by most of the beta-lactamases tested except the metallo-beta-lactamases from Stenotrophomonas maltophilia and Bacteroides fragilis. R-95867 showed potent bactericidal activity against S. aureus and Escherichia coli. Penicillin-binding proteins 1 and 4 of S. aureus and 1Bs, 2, 3, and 4 of E. coli had high affinities for R-95867. The in vivo efficacy of CS-834 was evaluated in murine systemic infections caused by 16 strains of gram-positive and -negative pathogens. The efficacy of CS-834 was in many cases superior to those of cefteram pivoxil, cefpodoxime proxetil, cefdinir, and cefditoren pivoxil, especially against infections caused by S. aureus, penicillin-resistant S. pneumoniae, E. coli, Citrobacter freundii, and Proteus vulgaris. Among the drugs tested, CS-834 showed the highest efficacy against experimental pneumonia in mice caused by penicillin-resistant S. pneumoniae.
- Published
- 1998
29. Association of Epstein-Barr virus with gastric carcinoma with lymphoid stroma
- Author
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K, Oda, J, Tamaru, T, Takenouchi, A, Mikata, M, Nunomura, N, Saitoh, H, Sarashina, and N, Nakajima
- Subjects
Adult ,Aged, 80 and over ,Male ,Ribosomal Proteins ,Base Sequence ,Lymphoid Tissue ,Carcinoma ,Molecular Sequence Data ,RNA-Binding Proteins ,Genome, Viral ,Middle Aged ,Polymerase Chain Reaction ,Blotting, Southern ,Stomach Neoplasms ,hemic and lymphatic diseases ,Molecular Probes ,Carcinoma, Squamous Cell ,Humans ,Female ,In Situ Hybridization ,Aged ,Research Article - Abstract
Epstein-Barr virus (EBV) has been detected in lymphoepithelioma of nasopharynx and lymphoepitheliomalike carcinomas in various organs. To clarify the association of EBV with gastric carcinoma with lymphoid stroma, which often resembles lymphoepithelioma, the authors examined 22 such cases by polymerase chain reaction and in situ hybridization techniques. In 18 informative cases, EBV DNA was detected by polymerase chain reaction in 14 (77.8%) cases, including lymph node metastases. EBV RNA was detected within the nuclei of carcinoma cells by in situ hybridization in all cases that were positive by polymerase chain reaction. Infiltrating lymphocytes and normal epithelia adjacent to carcinoma were EBV-negative. Southern blot analysis indicated clonal proliferation of tumor cells and episomal form of EBV. These findings suggest that EBV infection occurs before transformation and may be related to oncogenesis of EBV-associated gastric carcinoma.
- Published
- 1993
30. Holes in the Valence Band of Superconducting Boron-Doped Diamond Film Studied by Soft X-ray Absorption and Emission Spectroscopy
- Author
-
Tamio Oguchi, Isao Sakaguchi, Nobuyoshi Yamada, Jin Nakamura, Rupert C. C. Perera, Kozo Okada, Hiroshi Kawarada, David L. Ederer, Yoshihiko Takano, Kazuhiko Kuroki, T. Takenouchi, and Masanori Nagao
- Subjects
X-ray absorption spectroscopy ,Materials science ,Absorption spectroscopy ,Doping ,Fermi level ,General Physics and Astronomy ,Diamond ,engineering.material ,Condensed Matter::Materials Science ,symbols.namesake ,Condensed Matter::Superconductivity ,Excited state ,engineering ,symbols ,Condensed Matter::Strongly Correlated Electrons ,Emission spectrum ,Atomic physics ,Electronic band structure - Abstract
Carbon- and boron-2 p states of superconducting and non-superconducting boron-doped diamond (BDD) samples are measured using soft X-ray emission and absorption spectroscopy (XES and XAS) near C- and B-K edges. The electronic structure of B 2 p does not show a marked boron-doping dependence, except that a considerable amount of in-gap state in empty states is observed. In C-K XAS spectra, two peaks H and I are observed around the Fermi level. The H peak is attributed to 2 p state of carbon first-nearest neighboring to the dopant boron atoms (1NN-C), and the I peak to 2 p state of carbon further than 1NN-C from an impurity boron. Incoherent (normal-excitation) XES spectra do not show a large chemical shift by B-doping, but its intensity just below the valence band maximum (VBM) decreases with B-doping. It cannot be interpreted within a simple rigid band model. An elastic peak of I -excited C-K XES spectrum and the width of I -peak of C-K XAS spectrum suggest that impurity state is localized in non-supercond...
- Published
- 2008
31. 1P160 Surface rupture analysis of sarcomere structure of skeltal muscle myofibrils by laser tweezer technique
- Author
-
M. Ohtsuki, Y. Kunioka, T. Yamada, T. Takenouchi, and D. Miyashiro
- Subjects
Surface rupture ,Materials science ,Optical tweezers ,Biophysics ,Anatomy ,Myofibril ,Sarcomere - Published
- 2004
32. Surface structures of skeletal muscle myofibrils studied by use of laser tweezer technique
- Author
-
T. Takenouchi, N. Miyake, T. Yamada, M. Ohtsuki, T. Kayamori, and Y. Kunioka
- Subjects
Surface (mathematics) ,Crystallography ,medicine.anatomical_structure ,Materials science ,Optical tweezers ,medicine ,Biophysics ,Skeletal muscle ,Myofibril - Published
- 2003
33. Renin-Angiotensin-Aldosterone System in Hyperthyroidism
- Author
-
S, Fukuchi, T, Takenouchi, K, Nakajima, T, Sakurada, and S, Saito
- Subjects
Information retrieval ,Computer science ,business.industry ,Angiotensin II ,Posture ,Hyperthyroidism ,Hypopituitarism ,Text mining ,Hypothyroidism ,Furosemide ,Hypertension ,Myxedema ,Renin ,Humans ,Female ,business ,Aldosterone - Published
- 1973
34. [Untitled]
- Author
-
T. Takenouchi, K. Kitamura, Y. Iwanami, and M. Funazaki
- Subjects
Applied Mathematics - Published
- 1984
35. Calculation of probability of paternity using DNA sequences
- Author
-
D W, Gjertson, M R, Mickey, J, Hopfield, T, Takenouchi, and P I, Terasaki
- Subjects
Genetic Markers ,Male ,Blotting, Southern ,Phenotype ,Humans ,Paternity ,DNA ,Probability ,Research Article - Abstract
This paper formally incorporates allele measurement error into the Essen-Möller version of the probability of paternity. For highly polymorphic genetic systems, an approximate solution to the problem is developed resulting in simple formulas. The DNA sequence of the D14S1 region provided a practical example for testing this approximation. For these sequences, allelic uncertainty arises from determining length of DNA fragments from mobility in gel electrophoresis. D14S1 and standard test results from 35 paternity cases establish the validity of our computational method.
- Published
- 1988
36. ASXL1-related Bohring-Optiz Syndrome complicated by Persistent Neonatal Pulmonary Hypertension and Abnormal Alveoli Formation.
- Author
-
Arioka M, Nakamura S, Nishioka K, Inoue K, Nakao Y, Miyai Y, Morita H, Koyano K, Takenouchi T, Yasuda S, Chiba Y, Iwase T, Ueno M, and Kusaka T
- Abstract
Bohring-Opitz syndrome (BOS) is a rare disease with a characteristic facial appearance and limb position. This report describes a case of BOS complicated by persistent pulmonary hypertension of the newborn (PPHN) and formation of abnormal alveoli that was confirmed by autopsy. A female neonate was born by cesarean section at 37 weeks and 2 days of gestation and found to have a nevus flammeus, exophthalmos, abnormal palate, retraction of the mandible, and a posture characteristic of BOS. The patients had severe PPHN requiring inhalation of nitric oxide. Genetic testing revealed a de novo frameshift variant in ASXL1. Autopsy revealed that the lung was at the saccular stage, equivalent to 28-34 weeks of gestation. This is the first report to present pathological evidence of immaturity of the lung that may be associated with PPHN in a patient with BOS caused by a variant in ASXL1., Competing Interests: Declaration of Competing Interest The authors declare no conflict of interests., (Copyright © 2024. Published by Elsevier Masson SAS.)
- Published
- 2024
- Full Text
- View/download PDF
37. Digital clubbing without hypoxia for lysinuric protein intolerance.
- Author
-
Watanabe D, Tsujioka Y, Nakato D, Yamada M, Suzuki H, Ohnishi T, Tamai N, Kijima T, Takenouchi T, Miya F, Narumi S, and Kosaki K
- Subjects
- Humans, Male, Child, Lung Diseases, Interstitial genetics, Lung Diseases, Interstitial pathology, Osteoarthropathy, Secondary Hypertrophic genetics, Osteoarthropathy, Secondary Hypertrophic pathology, Hypoxia genetics, Large Neutral Amino Acid-Transporter 1 genetics, Large Neutral Amino Acid-Transporter 1 metabolism, Mutation, Amino Acid Transport System y+L, Amino Acid Metabolism, Inborn Errors genetics, Amino Acid Metabolism, Inborn Errors pathology
- Abstract
Digital clubbing is characterized by bulbous enlargement of the terminal segments of the fingers. Hypotheses including hypoxia have been proposed for the pathogenesis of digital clubbing, but the exact pathogenesis of digital clubbing is still uncertain. Lysinuric protein intolerance (LPI) is caused by pathogenic variants in SLC7A7 and is often associated with interstitial lung disease. Previously two patients of LPI with digital clubbing but without hypoxia have been reported. It is unclear whether digital clubbing in LPI is secondary to hypoxia or directly related to SLC7A7 deficiency. Here we report a 6-year-old Japanese boy presented with digital clubbing without hypoxia. He had episodic vomiting, each episode consisting of a single vomiting event occurring once a month, and his growth had been delayed. He had interstitial lung disease and hepatomegaly. He had compound heterozygous pathogenic variants in the SLC7A7, leading to the diagnosis of LPI. Together with the two previously reported patients mentioned above, we conclude that digital clubbing can occur in the absence of hypoxia. Digital clubbing in the absence of hypoxia has been observed in two genetic disorders related to prostaglandin (PG) E2, HPGD and SLCO2A1. PGE2 synthesis is primarily regulated by the cyclooxygenase 2, which plays a critical role in the control of inflammation. A high urine PGE level in the patient was compatible with the notion that PGE2 production may be increased in LPI. The occurrence of digital clubbing in the absence of hypoxia in LPI patients with SLC7A7 may be attributed to the mechanism of increased PGE2 production., (Copyright © 2024 The Authors. Published by Elsevier Masson SAS.. All rights reserved.)
- Published
- 2024
- Full Text
- View/download PDF
38. Establishment and characterization of an immortalized red river hog blood-derived macrophage cell line.
- Author
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Takenouchi T, Masujin K, Ikeda R, Haraguchi S, Suzuki S, Uenishi H, Onda E, and Kokuho T
- Subjects
- Animals, Swine, Coculture Techniques, Cell Line, Transformed, Cell Line, African Swine Fever virology, African Swine Fever immunology, Macrophages immunology, Macrophages virology, Macrophages metabolism, African Swine Fever Virus
- Abstract
Red river hogs (RRHs) ( Potamochoerus porcus ), a wild species of Suidae living in Africa with a major distribution in the Guinean and Congolian forests, are natural reservoirs of African swine fever virus (ASFV) and typically are asymptomatic. Since blood and tissue macrophages of suid animals are target cell lineages of ASFV, RRH-derived macrophages are expected to play an important role in suppressing disease development in infected individuals. In the present study, we successfully isolated RRH-derived blood macrophages using co-culture techniques of RRH blood cells with porcine kidney-derived feeder cells and immortalized them by transferring SV40 large T antigen and porcine telomerase reverse transcriptase genes. The newly established macrophage cell line of the RRH-derived blood cell origin (RZJ/IBM) exhibited an Iba1-, CD172a-, and CD203a-positive typical macrophage-like phenotype and up-regulated the phosphorylation of nuclear factor-κB p65 subunit and p38 mitogen-activated protein kinase in response to the bacterial cell wall components, lipopolysaccharide (LPS) and muramyl dipeptide. In addition, RZJ/IBM cells produced the precursor form of interleukin (IL)-1β and IL-18 upon a stimulation with LPS, leading to the conversion of IL-18, but not IL-1β, into the mature form. Time-lapse live cell imaging with pHrodo dye-conjugated Escherichia coli BioParticles demonstrated the phagocytotic activity of RZJ/IBM cells. It is important to note that RZJ/IBM cells are clearly susceptible to ASFV infection and support viral replication in vitro . Therefore, the RZJ/IBM cell line provides a unique model for investigating the pathogenesis of ASFV., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. The author(s) declared that they were an editorial board member of Frontiers, at the time of submission. This had no impact on the peer review process and the final decision., (Copyright © 2024 Takenouchi, Masujin, Ikeda, Haraguchi, Suzuki, Uenishi, Onda and Kokuho.)
- Published
- 2024
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- View/download PDF
39. Biallelic structural variants in three patients with ERCC8-related Cockayne syndrome and a potential pitfall of copy number variation analysis.
- Author
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Watanabe D, Okamoto N, Kobayashi Y, Suzuki H, Kato M, Saitoh S, Kanemura Y, Takenouchi T, Yamada M, Nakato D, Sato M, Tsunoda T, Kosaki K, and Miya F
- Subjects
- Humans, Male, Female, Exons genetics, Exome Sequencing, Alleles, Child, Child, Preschool, Cockayne Syndrome genetics, DNA Copy Number Variations, DNA Repair Enzymes genetics, Transcription Factors genetics
- Abstract
Cockayne syndrome (CS) is a rare autosomal recessive disorder caused by mutations in ERCC8 or ERCC6. Most pathogenic variants in ERCC8 are single nucleotide substitutions. Structural variants (SVs) have been reported in patients with ERCC8-related CS. However, comprehensive molecular detection, including SVs of ERCC8, in CS patients remains problematic. Herein, we present three Japanese patients with ERCC8-related CS in whom causative SVs were identified using whole-exome-based copy number variation (CNV) detection tools. One patient showed compound heterozygosity for a 259-kb deletion and a deletion of exon 4 which has previously been reported as an Asia-specific variant. The other two patients were homozygous for the same exon 4 deletion. The exon 4 deletion was detected only by the ExomeDepth software. Intrigued by the discrepancy in the detection capability of various tools for the SVs, we evaluated the analytic performance of four whole-exome-based CNV detection tools using an exome data set from 337 healthy individuals. A total of 1,278,141 exons were predicted as being affected by the 4 CNV tools. Interestingly 95.1% of these affected exons were detected by one tool alone. Thus, we expect that the use of multiple tools may improve the detection rate of SVs from aligned exome data., (© 2024. The Author(s).)
- Published
- 2024
- Full Text
- View/download PDF
40. Identification of Nonsynonymous SNPs in Immune-Related Genes Associated with Pneumonia Severity in Pigs.
- Author
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Shinkai H, Suzuki K, Itoh T, Yoshioka G, Takenouchi T, Kitazawa H, and Uenishi H
- Subjects
- Swine, Receptors, Pattern Recognition genetics, Receptors, Pattern Recognition immunology, Male, Female, Genotype, Alleles, Severity of Illness Index, Pneumonia genetics, Pneumonia immunology, Pneumonia microbiology, Pneumonia veterinary, Polymorphism, Single Nucleotide, Swine Diseases genetics, Swine Diseases immunology, Swine Diseases microbiology
- Abstract
We previously showed that several polymorphisms in genes encoding pattern recognition receptors that cause amino acid substitutions alter pathogen recognition ability and disease susceptibility in pigs. In this study, we expanded our analysis to a wide range of immune-related genes and investigated polymorphism distribution and its influence on pneumonia in multiple commercial pig populations. Among the polymorphisms in 42 genes causing 634 amino acid substitutions extracted from the swine genome database, 80 in 24 genes were found to have a minor allele frequency of at least 10% in Japanese breeding stock pigs via targeted resequencing. Of these, 62 single nucleotide polymorphisms (SNPs) in 23 genes were successfully genotyped in 862 pigs belonging to four populations with data on pneumonia severity. Association analysis using a generalized linear mixed model revealed that 12 SNPs in nine genes were associated with pneumonia severity. In particular, SNPs in the cellular receptor for immunoglobulin G FCGR2B and the intracellular nucleic acid sensors IFI16 and LRRFIP1 were found to be associated with mycoplasmal pneumonia of swine or porcine pleuropneumonia in multiple populations and may therefore have wide applications in the improvement of disease resistance in pigs. Functional analyses at the cellular and animal levels are required to clarify the mechanisms underlying the effects of these SNPs on disease susceptibility.
- Published
- 2024
- Full Text
- View/download PDF
41. Biallelic loss-of-function variants in the centriolar protein CCP110 leads to a ciliopathy-like phenotype.
- Author
-
Suzuki H, Muramatsu Y, Miya F, Asada H, Yamada M, Nishimura G, Kosaki K, and Takenouchi T
- Subjects
- Humans, Male, Infant, Loss of Function Mutation, Microtubule-Associated Proteins genetics, Alleles, Cytoskeletal Proteins, Phenotype, Ciliopathies genetics, Ciliopathies pathology, Cell Cycle Proteins genetics
- Abstract
CCP110 (centriolar coiled coil protein 110, also known as CP110) is one of the essential proteins localized in the centrosome that plays critical roles in the regulation of the cell cycle and also in the initiation of ciliogenesis. So far, no human congenital disorders have been identified to be associated with pathogenic variants of CCP110. Mice with biallelic loss-of-function variants of Ccp110 (Ccp110
-/- ) are known to manifest multiple organ defects, including a small body size, polydactyly, omphalocele, congenital heart defects, cleft palate, short ribs, and a small thoracic cage, a pattern of abnormalities closely resembling that in "ciliopathies" in humans. Herein, we report a 7-month-old male infant who presented with growth failure and skeletal abnormalities, including a narrow thorax and severe brachydactyly. Trio exome analysis of the genomic DNA of the patient and his parents showed that the patient was a compound heterozygote for truncating variants of CCP110, including a frameshift variant NM_001323572.2:c.856_857del, p.(Val286Leufs*5) inherited from the father, and a nonsense variant NM_001323572.2:c.1129C>T, p.(Arg377*) inherited from the mother. The strikingly similar pattern of malformations between Ccp110-/- mice and the 7-month-old male infant reported herein carrying unequivocal truncating CCP110 variants strongly supports the contention that CCP110 is a novel disease-causative gene., (Copyright © 2024 The Authors. Published by Elsevier Masson SAS.. All rights reserved.)- Published
- 2024
- Full Text
- View/download PDF
42. Successful skipping of abnormal pseudoexon by antisense oligonucleotides in vitro for a patient with beta-propeller protein-associated neurodegeneration.
- Author
-
Yamada M, Maeta K, Suzuki H, Kurosawa R, Takenouchi T, Awaya T, Ajiro M, Takeuchi A, Nishio H, Hagiwara M, Miya F, Matsuo M, and Kosaki K
- Subjects
- Female, Humans, Adolescent, Mutation, Exons genetics, Carrier Proteins genetics, Oligonucleotides, Antisense genetics, Oligonucleotides, Antisense pharmacology, RNA Splicing
- Abstract
Pathogenic variants in WDR45 on chromosome Xp11 cause neurodegenerative disorder beta-propeller protein-associated neurodegeneration (BPAN). Currently, there is no effective therapy for BPAN. Here we report a 17-year-old female patient with BPAN and show that antisense oligonucleotide (ASO) was effective in vitro. The patient had developmental delay and later showed extrapyramidal signs since the age of 15 years. MRI findings showed iron deposition in the globus pallidus and substantia nigra on T2 MRI. Whole genome sequencing and RNA sequencing revealed generation of pseudoexon due to inclusion of intronic sequences triggered by an intronic variant that is remote from the exon-intron junction: WDR45 (OMIM #300526) chrX(GRCh37):g.48935143G > C, (NM_007075.4:c.235 + 159C > G). We recapitulated the exonization of intron sequences by a mini-gene assay and further sought antisense oligonucleotide that induce pseudoexon skipping using our recently developed, a dual fluorescent splicing reporter system that encodes two fluorescent proteins, mCherry, a transfection marker designed to facilitate evaluation of exon skipping and split eGFP, a splicing reaction marker. The results showed that the 24-base ASO was the strongest inducer of pseudoexon skipping. Our data presented here have provided supportive evidence for in vivo preclinical studies., (© 2024. The Author(s).)
- Published
- 2024
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43. Alopecia neoplastica: A manifestation of metastatic breast cancer.
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Kitayama S, Katsuumi K, Takatsuka S, Kanbayashi C, Shimizu T, and Takenouchi T
- Abstract
We herein report a typical case of alopecia neoplastica secondary to breast cancer. Alopecia neoplastica is a rare form of alopecia resulting from metastasis of a primary tumour to the scalp and is often misdiagnosed as alopecia areata., Competing Interests: None to declare., (© 2023 The Authors. Skin Health and Disease published by John Wiley & Sons Ltd on behalf of British Association of Dermatologists.)
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- 2023
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44. Gain-of-function MYCN causes a megalencephaly-polydactyly syndrome manifesting mirror phenotypes of Feingold syndrome.
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Nishio Y, Kato K, Tran Mau-Them F, Futagawa H, Quélin C, Masuda S, Vitobello A, Otsuji S, Shawki HH, Oishi H, Thauvin-Robinet C, Takenouchi T, Kosaki K, Takahashi Y, and Saitoh S
- Subjects
- Mice, Animals, Humans, Female, Gain of Function Mutation, N-Myc Proto-Oncogene Protein genetics, Phenotype, Microcephaly genetics, Polydactyly genetics, Megalencephaly genetics, Eyelids abnormalities, Intellectual Disability, Tracheoesophageal Fistula, Limb Deformities, Congenital
- Abstract
MYCN, a member of the MYC proto-oncogene family, regulates cell growth and proliferation. Somatic mutations of MYCN are identified in various tumors, and germline loss-of-function variants are responsible for Feingold syndrome, characterized by microcephaly. In contrast, one megalencephalic patient with a gain-of-function variant in MYCN, p.Thr58Met, has been reported, and additional patients and pathophysiological analysis are required to establish the disease entity. Herein, we report two unrelated megalencephalic patients with polydactyly harboring MYCN variants of p.Pro60Leu and Thr58Met, along with the analysis of gain-of-function and loss-of-function Mycn mouse models. Functional analyses for MYCN-Pro60Leu and MYCN-Thr58Met revealed decreased phosphorylation at Thr58, which reduced protein degradation mediated by FBXW7 ubiquitin ligase. The gain-of-function mouse model recapitulated the human phenotypes of megalencephaly and polydactyly, while brain analyses revealed excess proliferation of intermediate neural precursors during neurogenesis, which we determined to be the pathomechanism underlying megalencephaly. Interestingly, the kidney and female reproductive tract exhibited overt morphological anomalies, possibly as a result of excess proliferation during organogenesis. In conclusion, we confirm an MYCN gain-of-function-induced megalencephaly-polydactyly syndrome, which shows a mirror phenotype of Feingold syndrome, and reveal that MYCN plays a crucial proliferative role, not only in the context of tumorigenesis, but also organogenesis., Competing Interests: Declaration of interests The authors declare no competing interests., (Copyright © 2023 The Authors. Published by Elsevier Inc. All rights reserved.)
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- 2023
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45. Systemic therapy for Asian patients with advanced BRAF V600-mutant melanoma in a real-world setting: A multi-center retrospective study in Japan (B-CHECK-RWD study).
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Namikawa K, Ito T, Yoshikawa S, Yoshino K, Kiniwa Y, Ohe S, Isei T, Takenouchi T, Kato H, Mizuhashi S, Fukushima S, Yamamoto Y, Inozume T, Fujisawa Y, Yamasaki O, Nakamura Y, Asai J, Maekawa T, Funakoshi T, Matsushita S, Nakano E, Oashi K, Kato J, Uhara H, Miyagawa T, Uchi H, Hatta N, Tsutsui K, Maeda T, Matsuya T, Yanagisawa H, Muto I, Okumura M, Ogata D, and Yamazaki N
- Subjects
- Humans, CTLA-4 Antigen, Retrospective Studies, Programmed Cell Death 1 Receptor, Japan, Protein Kinase Inhibitors therapeutic use, Mitogen-Activated Protein Kinase Kinases, Proto-Oncogene Proteins B-raf genetics, Melanoma drug therapy, Melanoma genetics
- Abstract
Background: Anti-PD-1-based immunotherapy is considered a preferred first-line treatment for advanced BRAF V600-mutant melanoma. However, a recent international multi-center study suggested that the efficacy of immunotherapy is poorer in Asian patients in the non-acral cutaneous subtype. We hypothesized that the optimal first-line treatment for Asian patients may be different., Methods: We retrospectively collected data of Asian patients with advanced BRAF V600-mutant melanoma treated with first-line BRAF/MEK inhibitors (BRAF/MEKi), anti-PD-1 monotherapy (Anti-PD-1), and nivolumab plus ipilimumab (PD-1/CTLA-4) between 2016 and 2021 from 28 institutions in Japan., Results: We identified 336 patients treated with BRAF/MEKi (n = 236), Anti-PD-1 (n = 64) and PD-1/CTLA-4 (n = 36). The median follow-up duration was 19.9 months for all patients and 28.6 months for the 184 pa tients who were alive at their last follow-up. For patients treated with BRAF/MEKi, anti-PD-1, PD-1/CTLA-4, the median ages at baseline were 62, 62, and 53 years (p = 0.03); objective response rates were 69%, 27%, and 28% (p < 0.001); median progression-free survival (PFS) was 14.7, 5.4, and 5.8 months (p = 0.003), and median overall survival (OS) was 34.6, 37.0 months, and not reached, respectively (p = 0.535). In multivariable analysis, hazard ratios (HRs) for PFS of Anti-PD-1 and PD-1/CTLA-4 compared with BRAF/MEKi were 2.30 (p < 0.001) and 1.38 (p = 0.147), and for OS, HRs were 1.37 (p = 0.111) and 0.56 (p = 0.075), respectively. In propensity-score matching, BRAF/MEKi showed a tendency for longer PFS and equivalent OS with PD-1/CTLA-4 (HRs for PD-1/CTLA-4 were 1.78 [p = 0.149]) and 1.03 [p = 0.953], respectively). For patients who received second-line treatment, BRAF/MEKi followed by PD-1/CTLA-4 showed poor survival outcomes., Conclusions: The superiority of PD-1/CTLA-4 over BRAF/MEKi appears modest in Asian patients. First-line BRAF/MEKi remains feasible, but it is difficult to salvage at progression. Ethnicity should be considered when selecting systemic therapies until personalized biomarkers are available in daily practice. Further studies are needed to establish the optimal treatment sequence for Asian patients., (© 2023 The Authors. Cancer Medicine published by John Wiley & Sons Ltd.)
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- 2023
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46. Efficacy of salvage therapies for advanced acral melanoma after anti-PD-1 monotherapy failure: a multicenter retrospective study of 108 Japanese patients.
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Mori T, Namikawa K, Yamazaki N, Kiniwa Y, Yamasaki O, Yoshikawa S, Inozume T, Kato H, Nakai Y, Fukushima S, Takenouchi T, Maekawa T, Matsushita S, Otsuka A, Nomura M, Baba N, Isei T, Saito S, Fujimoto N, Tanaka R, Kaneko T, Kuwatsuka Y, Matsuya T, Nagase K, Onishi M, Onuma T, and Nakamura Y
- Abstract
Background: Anti-programmed cell death protein 1 (PD-1) monotherapy is one of the standard systemic therapies for advanced melanoma; however, the efficacy of salvage systemic therapies after PD-1 monotherapy failure (PD-1 MF), particularly in acral melanoma (AM), the main clinical melanoma type in Japanese patients, is unclear. This study aimed to investigate the efficacy of salvage systemic therapies in Japanese patients with AM after PD-1 MF., Patients and Methods: The study included 108 patients with advanced AM (palm and sole, 72; nail apparatus, 36) who underwent salvage systemic therapy at 24 Japanese institutions. We mainly assessed the objective response rate (ORR), progression-free survival (PFS), and overall survival (OS)., Results: Thirty-six (33%) patients received ipilimumab, 23 (21%) received nivolumab and ipilimumab (nivo/ipi), 10 (9%) received cytotoxic chemotherapy, 4 (4%) received BRAF and MEK inhibitors (BRAFi/MEKi), and the remaining 35 (32%) continued with PD-1 monotherapy after disease progression. The ORRs in the ipilimumab, nivo/ipi, cytotoxic chemotherapy, and BRAFi/MEKi groups were 8, 17, 0, and 100%, respectively. The nivo/ipi group showed the longest OS (median, 18.9 months); however, differences in ORR, PFS, and OS between the groups were insignificant. The OS in the nivo/ipi group was higher in the palm and sole groups than in the nail apparatus group (median: not reached vs. 8.7 months, p < 0.001). Cox multivariate analysis demonstrated that nail apparatus melanoma independently predicted unfavorable PFS and OS ( p = 0.006 and 0.001). The total OS (from PD-1 monotherapy initiation to death/last follow-up) was insignificant between the groups., Conclusion: Nivo/ipi was not more effective than cytotoxic chemotherapy and ipilimumab after PD-1 MF in patients with advanced AM. The prognosis after PD-1 MF would be poorer for nail apparatus melanoma than for palm and sole melanoma., Competing Interests: TMo and SS have received honoraria from Ono Pharma. KeN has served as a consultant and/or received honoraria from Novartis, Bristol-Myers Squibb (BMS), Merck Sharp & Dohme (MSD), Novartis, and Ono Pharma. NY has received research funding from BMS, MSD, Novartis, Ono Pharma, and Takara Bio, and has served as a consultant and/or received honoraria from BMS, MSD, Novartis, and Ono Pharma. YKi, SY, HK, and NF have received honoraria from Novartis and Ono Pharma. OY has received research funding and/or honoraria from Ono Pharma. TIn has received honoraria from BMS, MSD, and Ono Pharma. SF has received research funding from Ono Pharma and honoraria from BMS, MSD, and Novartis. TT, TMae, and SM have received honoraria from BMS, MSD, Novartis, and Ono Pharma. AO has served as a consultant and/or received honoraria from BMS, MSD, Novartis, and Ono Pharma, and has received research funding from Eisai. TIs has served as a consultant and/or received honoraria from Ono Pharma, Pfizer, BMS, and Novartis. YNakamura has served as a consultant and/or received honoraria from BMS, MSD, Maruho, Ono Pharma, Sanofi, Sun Pharma, Tanabe Mitsubishi Pharma, and Torii. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2023 Mori, Namikawa, Yamazaki, Kiniwa, Yamasaki, Yoshikawa, Inozume, Kato, Nakai, Fukushima, Takenouchi, Maekawa, Matsushita, Otsuka, Nomura, Baba, Isei, Saito, Fujimoto, Tanaka, Kaneko, Kuwatsuka, Matsuya, Nagase, Onishi, Onuma and Nakamura.)
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- 2023
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47. Heterozygous loss-of-function DHX9 variants are associated with neurodevelopmental disorders: Human genetic and experimental evidences.
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Yamada M, Nitta Y, Uehara T, Suzuki H, Miya F, Takenouchi T, Tamura M, Ayabe S, Yoshiki A, Maeno A, Saga Y, Furuse T, Yamada I, Okamoto N, Kosaki K, and Sugie A
- Subjects
- Animals, Humans, Mice, DEAD-box RNA Helicases genetics, Human Genetics, Neoplasm Proteins genetics, RNA genetics, RNA Helicases, Intellectual Disability genetics, Neurodevelopmental Disorders genetics
- Abstract
DExH-box helicases are involved in unwinding of RNA and DNA. Among the 16 DExH-box genes, monoallelic variants of DHX16, DHX30, DHX34, and DHX37 are known to be associated with neurodevelopmental disorders. In particular, DHX30 is well established as a causative gene for neurodevelopmental disorders. Germline variants of DHX9, the closest homolog of DHX30, have not been reported until now as being associated with congenital disorders in humans, except that one de novo heterozygous variant, p.(Arg1052Gln) of the gene was identified during comprehensive screening in a patient with autism; unfortunately, the phenotypic details of this individual are unknown. Herein, we report a patients with a heterozygous de novo missense variant, p.(Gly414Arg) of DHX9 who presented with a short stature, intellectual disability, and ventricular non-compaction cardiomyopathy. The variant was located in the glycine codon of the ATP-binding site, G-C-G-K-T. To assess the pathogenicity of these variants, we generated transgenic Drosophila lines expressing human wild-type and mutant DHX9 proteins: 1) the mutant proteins showed aberrant localization both in the nucleus and the cytoplasm; 2) ectopic expression of wild-type protein in the visual system led to the rough eye phenotype, whereas expression of the mutant proteins had minimal effect; 3) overexpression of the wild-type protein in the retina led to a reduction in axonal numbers, whereas expression of the mutant proteins had a less pronounced effect. Furthermore, in a gene-editing experiment of Dhx9 G416 to R416, corresponding to p.(Gly414Arg) in humans, heterozygous mice showed a reduced body size, reduced emotionality, and cardiac conduction abnormality. In conclusion, we established that heterozygosity for a loss-of-function variant of DHX9 can lead to a new neurodevelopmental disorder., Competing Interests: Declaration of competing interest The authors have no conflicts of interest to disclose., (Copyright © 2023. Published by Elsevier Masson SAS.)
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- 2023
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48. Aging trends in skin cancer: A long-term observational study in Japan.
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Nakamura A, Kataoka K, Takatsuka S, and Takenouchi T
- Abstract
Competing Interests: None disclosed.
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- 2023
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49. Oculofaciocardiodental syndrome caused by a novel BCOR variant.
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Yamashita T, Hotta J, Jogu Y, Sakai E, Ono C, Bamba H, Suzuki H, Yamada M, Takenouchi T, Kosaki K, Yorifuji T, Hamazaki T, and Seto T
- Abstract
Oculofaciocardiodental syndrome is caused by variants in the BCL6 corepressor (BCOR) gene. We identified a novel heterozygous frameshift variant, NM_001123385.2(BCOR):c.2326del, that arose de novo in a Japanese girl with characteristic facial features, congenital heart disease, bilateral syndactyly of toes 2 and 3, congenital cataracts, dental abnormalities, and mild intellectual disability. Reports of BCOR variants are rare, and further case accumulation is warranted., (© 2023. The Author(s).)
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- 2023
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50. Parkinsonism in spinocerebellar ataxia with axonal neuropathy caused by adult-onset COA7 variants: a case report.
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Ouchi S, Ishii K, Kosaki K, Suzuki H, Yamada M, Takenouchi T, and Tamaoka A
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- Humans, Adult, Middle Aged, Mutation, Charcot-Marie-Tooth Disease genetics, Spinocerebellar Ataxias complications, Spinocerebellar Ataxias diagnostic imaging, Spinocerebellar Ataxias genetics, Parkinsonian Disorders complications, Parkinsonian Disorders diagnostic imaging, Parkinsonian Disorders genetics
- Abstract
Background: Individuals with variants of cytochrome c oxidase assembly factor 7 (COA7), a mitochondrial functional-related gene, exhibit symptoms of spinocerebellar ataxia with axonal neuropathy before the age of 20. However, COA7 variants with parkinsonism or adult-onset type cases have not been described., Case Presentation: We report the case of a patient who developed cerebellar symptoms and slowly progressive sensory and motor neuropathy in the extremities, similar to Charcot-Marie-Tooth disease, at age 30, followed by parkinsonism at age 58. Exome analysis revealed COA7 missense mutation in homozygotes (NM_023077.2:c.17A > G, NP_075565.2: p.Asp6Gly). Dopamine transporter single-photon emission computed tomography using a
123 I-Ioflupane revealed clear hypo-accumulation in the bilateral striatum. However,123 I-metaiodobenzylguanidine myocardial scintigraphy showed normal sympathetic nerve function. Levodopa administration improved parkinsonism in this patient., Conclusions: COA7 gene variants may have caused parkinsonism in this case because mitochondrial function-related genes, such as parkin and PINK1, are known causative genes in some familial Parkinson's diseases., (© 2023. The Author(s).)- Published
- 2023
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