1. Anti-CD71 antibody immunohistochemistry in the diagnosis of acute myeloid leukemia, subtype acute erythroid leukemia with erythroid dominance (AML M6-Er), in a retrovirus-negative cat
- Author
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Ikki Mitsui, Takuro Kariya, Satoshi Suzuki, Naotaka Ogino, and Hiroyuki Ito
- Subjects
Pathology ,medicine.medical_specialty ,Feline immunodeficiency virus ,040301 veterinary sciences ,Spleen ,Cat Diseases ,Feline leukemia virus ,0403 veterinary science ,Diagnosis, Differential ,03 medical and health sciences ,0302 clinical medicine ,Antigens, CD ,Bone Marrow ,hemic and lymphatic diseases ,Receptors, Transferrin ,medicine ,Biomarkers, Tumor ,Animals ,CD20 ,General Veterinary ,biology ,business.industry ,Leukemia Virus, Feline ,Acute erythroid leukemia ,Myeloid leukemia ,Erythroid Hyperplasia ,04 agricultural and veterinary sciences ,biology.organism_classification ,medicine.disease ,Immunohistochemistry ,medicine.anatomical_structure ,Retroviridae ,030220 oncology & carcinogenesis ,biology.protein ,Cats ,Female ,Bone marrow ,Leukemia, Erythroblastic, Acute ,business ,Brief Communications - Abstract
CD71 is an immunohistochemical marker used in diagnosing acute myeloid leukemia (AML) M6-Er in humans; however, to our knowledge, it has not been reportedly used for immunohistochemistry in veterinary medicine. We evaluated the pathologic features of AML M6-Er in a retrovirus-negative cat and used CD71 to support the diagnosis. A 4-y-old spayed female Scottish Fold cat was presented with lethargy, anorexia, and fever. Whole-blood PCR assay results for pro feline leukemia virus/pro feline immunodeficiency virus and feline vector-borne diseases were negative. Early erythroid precursors were observed in the peripheral blood smear. Fine-needle aspiration of the enlarged spleen and splenic lymph node showed many early erythroid precursors. Bone marrow aspirate smears revealed erythroid hyperplasia with 68.4% erythroid lineage and 3.6% rubriblasts. Dysplastic cells infiltrated other organs. The patient was diagnosed with myelodysplastic syndrome, progressing to the early phase of AML M6-Er. The patient died on day 121 despite multidrug treatments. Postmortem examination revealed neoplastic erythroblasts infiltrating the bone marrow and other organs. Neoplastic cells were immunopositive for CD71 but immunonegative for CD3, CD20, granzyme B, von Willebrand factor, CD61, myeloperoxidase, and Iba-1. Although further studies are necessary for the application of CD71, our results supported the morphologic diagnosis of AML M6-Er.
- Published
- 2020