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Background: Dermatophytosis are the most common types of cutaneous fungal infection seen in human and animals affecting skin, hair and nails caused by dermatophytes. The diagnosis of dermatophytes is based on the clinical observation and laboratory diagnosis by direct microscopic examination and fungal cultures. The present study is undertaken to isolate different type of dermatophytes causing fungal infection.Methods: A prospective cross-sectional study design was used in a total of 90 clinically suspected cases of dermatophytic infection attending the out patient department of Kathmandu Medical College and Teaching hospital (KMCTH). Skin scraping, hair and nail samples were collected from the patients and were processed by direct microscopy and culture using standard protocol. Dermatophytes were identified based on the microscopic arrangement of microconidia and macroconidia.Results: Dermatophytosis was more common in the age group of 21-40 years and was more predominant among male with male to female ratio of 1.7: 1. Among the total clinically suspected cases of dermatophytosis, 53 were positive in direct microscopy and only a total of 20 were positive by culture. Most common clinical type observed in our study was Tinea corporis(25%) followed by Tinea cruris. Trichophyton rubrum(50%) was the commonest aetiological agent in majority of clinical types followed by Trychophyton mentagrophytes(35%). Conclusions: The study highlighted T. corporis followed by T. cruris and T. unguim as the most common clinical pattern of dermatophytosis with a male predominance and 21-40 years being the most affected age group. T. rubram was the most common aetiological agent causing dermatophytosis.Keywords: Dermatophytes; dermatophytosis; epidermophyton; tinea; trychophyton.

The study investigated the fungal composition of honey samples collected from six states in Southwestern Nigeria. Eighteen honey samples were screened in each state giving an overall total of 108 samples for the six states. The number of fungal species were determined and the microphotograph of colonial appearance of each was taken with Toshiba camera HD 1080P, 4X Digital Zoom. The morphological appearance of the fungal isolates as seen under the microscope were drawn and described.Seven species of fungi were isolated. The occurrence of the different species varied from one state to another. However, out of the seven species, only one, Trychophyton rubrum is pathogenic, and this is the first record of a pathogenic fungus from honey samples in Nigeria.

Outbreak of acute bovine dermatophytosis in a large scale dairy herd in Arusha region of Tanzania is described. Clinical history of the condition complimented with detailed examination of the affected animals (33.3%, 14/42) revealed that animals were pastured during the day and padlocked at night. The main clinical signs presented were gray-white, discrete, crusty, circumscribed extensive lesion all over the body. Skin scrapings were taken from the lesions for mites and fungal isolation and characterization. Pinched off lesion were examined in 30 % KOH preparations and scrapping were directly streaked on 0 Trichophyton Agar and incubated at 37 C. Bacteriological and fungal stain of the colonies revealed positive mycotic structures spherical, pyriform to calvate often of irregular shape which is characteristic of Trychophyton verrucosum. The isolation and morphological identification of T. verrucosum supported the clinical diagnosis. Considering the large number of animals involved, and the severity of the lesions, that necessitated veterinary intervention, it would be necessary to asses the prevalence and economic importance of the disease in dairy herd establishments in Tanzania. Arusha, Cattle, Tanzania, T. verrucosum