16 results on '"Varisco M"'
Search Results
2. From production planning flows to manufacturing operation management KPIs: linking ISO18828 & ISO22400 standards
- Author
-
Varisco, M., Deuse, J., Johnsson, C., Nöhring, F., Schiraldi, M.M., and Wöstmann, R.
- Published
- 2018
- Full Text
- View/download PDF
3. Electromyographic Evaluation of Masticatory Muscles in a Young Patient with Crossbite Treated with Rapid Palatal Expander: A Case Report
- Author
-
Maddalone, M, Nanussi, A, Varisco, M, Cortese, M, Scali, J, Bianco, E, Maddalone M., Nanussi A., Varisco M., Cortese M., Scali J., Bianco E., Maddalone, M, Nanussi, A, Varisco, M, Cortese, M, Scali, J, Bianco, E, Maddalone M., Nanussi A., Varisco M., Cortese M., Scali J., and Bianco E.
- Abstract
Aim and objective: The purpose of this work is to investigate by electromyographic (EMG) surface analysis whether and how the variations in the occlusion due to the correction of the posterior crossbite using a rapid palatal expander (RPE) is accompanied by changes in the activity of the elevator muscles in the pediatric patients. Background: Posterior crossbite is a disgnathic jaw relationship common in patients undergoing growth. In the last 10 years, several studies demonstrated the effectiveness and reproducibility of surface EMG in the objective evaluation of temporal muscle and masseter activity and how this evidence can bean interesting aid in dental clinical practice. Case description: The case subject BF is an 11-year-old boy with a right I class relationship and a left II class relationship treated with a rapid palatal expansion protocol with a turn of the transversal screw twice a day (0.25 mm each turn) for a week. In this case report, EMG was used to evaluate the temporal muscle and masseter activity immediately before, immediately after, and 4 months after the RPE protocol. Conclusion: Four months after the treatment, a condition of well-being and neuromuscular equilibrium such as that of starting was preserved. Clinical significance: Electromyography is a noninvasive exam which evaluates the masticatory muscle activity by facial application of electrodes on masseter and temporal muscles. It can be an interesting aid in orthodontic clinical practice to evaluate preservation of a good muscular balance following orthodontic and orthognathic movements.
- Published
- 2020
4. From production planning flows to manufacturing operation management KPIs: linking ISO18828 & ISO22400 standards
- Author
-
Varisco, M, Deuse, J, Johnsson, C, Nöhring, F, Schiraldi, MM, Wöstmann, R, Varisco, M, Deuse, J, Johnsson, C, Nöhring, F, Schiraldi, MM, and Wöstmann, R
- Abstract
International standards are playing a key role in leading and shaping the smart manufacturing landscape. The integration and consistency among different standards is therefore essential to effectively support industrial automation evolution and to ensure their applicability. This paper focuses on the ISO18828 and ISO22400 standards, related to the production planning process and manufacturing, consequential phases in product lifecycle. In this paper the connections between the information related to production planning process (ISO18828) and the KPI main basic elements in manufacturing operation management (ISO22400) are analysed. The analysis aims at supporting the standards’ users, underlining the aspects that should be taken into account in order to consolidate and improve the considered lifecycle phases.
- Published
- 2018
5. Fashion luxury retail supply chain: Determining target stock levels and lost sale probability
- Author
-
Lucci, G., Massimiliano M. Schiraldi, and Varisco, M.
- Subjects
Base stock policy ,Fashion luxury industry ,Inventory control ,Supply chain ,Target stock level ,Settore ING-IND/17 - Impianti Industriali Meccanici
6. Electromyographic Evaluation of Masticatory Muscles in a Young Patient with Crossbite Treated with Rapid Palatal Expander: A Case Report
- Author
-
Marina Cortese, Jessica Scali, Edoardo Bianco, Marcello Maddalone, Alessandro Nanussi, Margherita Varisco, Maddalone, M, Nanussi, A, Varisco, M, Cortese, M, Scali, J, and Bianco, E
- Subjects
Male ,Palatal Expansion Technique ,Posture ,Temporal Muscle ,Gnathology ,Electromyography ,Temporal muscle ,Temporomandibular joint ,03 medical and health sciences ,0302 clinical medicine ,Occlusion ,medicine ,Orthodontic ,Humans ,Clinical significance ,Child ,General Dentistry ,Balance (ability) ,Orthodontics ,medicine.diagnostic_test ,Masseter Muscle ,Crossbite ,business.industry ,Reproducibility of Results ,030206 dentistry ,medicine.disease ,Masticatory force ,Masticatory Muscles ,business ,Malocclusion ,030217 neurology & neurosurgery - Abstract
Aim and objective The purpose of this work is to investigate by electromyographic (EMG) surface analysis whether and how the variations in the occlusion due to the correction of the posterior crossbite using a rapid palatal expander (RPE) is accompanied by changes in the activity of the elevator muscles in the pediatric patients. Background Posterior crossbite is a disgnathic jaw relationship common in patients undergoing growth. In the last 10 years, several studies demonstrated the effectiveness and reproducibility of surface EMG in the objective evaluation of temporal muscle and masseter activity and how this evidence can be an interesting aid in dental clinical practice. Case description The case subject BF is an 11-year-old boy with a right I class relationship and a left II class relationship treated with a rapid palatal expansion protocol with a turn of the transversal screw twice a day (0.25 mm each turn) for a week. In this case report, EMG was used to evaluate the temporal muscle and masseter activity immediately before, immediately after, and 4 months after the RPE protocol. Conclusion Four months after the treatment, a condition of well-being and neuromuscular equilibrium such as that of starting was preserved. Clinical significance Electromyography is a noninvasive exam which evaluates the masticatory muscle activity by facial application of electrodes on masseter and temporal muscles. It can be an interesting aid in orthodontic clinical practice to evaluate preservation of a good muscular balance following orthodontic and orthognathic movements. How to cite this article Maddalone M, Nanussi A, Varisco M, et al. Electromyographic Evaluation of Masticatory Muscles in a Young Patient with Crossbite Treated with Rapid Palatal Expander: A Case Report. J Contemp Dent Pract 2020;21(11):1279–1283.
- Published
- 2020
7. Gene Expression of Disease-related Genes in Alzheimer's Disease is Impaired by Tau Aggregation
- Author
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K. Dunville, Maria Claudia Caiazza, Martina Varisco, Antonino Cattaneo, C. Di Primio, A. Scarlatti, Laura Pancrazi, Giacomo Siano, Mario Costa, F. Cremisi, Siano, G., Varisco, M., Scarlatti, A., Caiazza, M. C., Dunville, K., Cremisi, F., Costa, M., Pancrazi, L., Di Primio, C., and Cattaneo, A.
- Subjects
Amyloid ,Amino Acid Transport System X-AG ,Active Transport, Cell Nucleus ,Prefrontal Cortex ,tau Proteins ,Biology ,Neurotransmission ,tauopathie ,Protein Aggregation, Pathological ,03 medical and health sciences ,Glutamatergic ,Settore BIO/06 - Anatomia Comparata e Citologia ,Mice ,0302 clinical medicine ,Downregulation and upregulation ,VGluT1 ,Structural Biology ,Alzheimer Disease ,Gene expression ,Animals ,Humans ,Prefrontal cortex ,Molecular Biology ,Embryonic Stem Cells ,030304 developmental biology ,Neurons ,0303 health sciences ,prefrontal cortex ,tau aggregation ,Glutamate receptor ,Brain ,Cell biology ,Gene Expression Regulation ,Tauopathies ,Synapses ,Vesicular Glutamate Transport Protein 1 ,gene expression ,Glutamatergic synapse ,030217 neurology & neurosurgery - Abstract
Neuronal hyperexcitability linked to an increase in glutamate signalling is a peculiar trait of the early stages of Alzheimer's disease (AD) and tauopathies, however, a progressive reduction in glutamate release follows in advanced stages. We recently reported that in the early phases of the neurodegenerative process, soluble, non-aggregated Tau accumulates in the nucleus and modulates the expression of disease-relevant genes directly involved in glutamatergic transmission, thus establishing a link between Tau instability and altered neurotransmission. Here we report that while the nuclear translocation of Tau in cultured cells is not impaired by its own aggregation, the nuclear amyloid inclusions of aggregated Tau abolish Tau-dependent increased expression of the glutamate transporter. Remarkably, we observed that in the prefrontal cortex (PFC) of AD patient brain, the glutamate transporter is upregulated at early stages and is downregulated at late stages. The Gene Set Enrichment Analysis indicates that the modulation of Tau-dependent gene expression along the disease progression can be extended to all protein pathways of the glutamatergic synapse. Together, this evidence links the altered glutamatergic function in the PFC during AD progression to the newly discovered function of nuclear Tau.
- Published
- 2020
8. Identification of an ERK Inhibitor as a Therapeutic Drug Against Tau Aggregation in a New Cell-Based Assay
- Author
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Giacomo Siano, Maria Claudia Caiazza, Ivana Ollà, Martina Varisco, Giuseppe Madaro, Valentina Quercioli, Mariantonietta Calvello, Antonino Cattaneo, Cristina Di Primio, Siano, G., Caiazza, M. C., Olla, I., Varisco, M., Madaro, G., Quercioli, V., Calvello, M., Cattaneo, A., and Di Primio, C.
- Subjects
0301 basic medicine ,Drug ,MAPK/ERK pathway ,media_common.quotation_subject ,Cell ,lcsh:RC321-571 ,03 medical and health sciences ,Cellular and Molecular Neuroscience ,0302 clinical medicine ,medicine ,Cytotoxicity ,lcsh:Neurosciences. Biological psychiatry. Neuropsychiatry ,Original Research ,CST ,media_common ,Tau biosensor ,Kinase ,Chemistry ,Drug discovery ,aggregation ,Cell biology ,ERK ,030104 developmental biology ,medicine.anatomical_structure ,Förster resonance energy transfer ,FRET ,Tau ,030217 neurology & neurosurgery ,Neuroscience ,Cell based - Abstract
Formation of Tau aggregates is a common pathological feature of tauopathies and their accumulation directly correlates with cytotoxicity and neuronal degeneration. Great efforts have been made to understand Tau aggregation and to find therapeutics halting or reversing the process, however, progress has been slowed due to the lack of a suitable method for monitoring Tau aggregation. We developed a cell-based assay allowing to detect and quantify Tau aggregation in living cells. The system is based on the FRET biosensor CST able to monitor the molecular dynamic of Tau aggregation in different cellular conditions. We probed candidate compounds that could block Tau hyperphosphorylation. In particular, to foster the drug discovery process, we tested kinase inhibitors approved for the treatment of other diseases. We identified the ERK inhibitor PD-901 as a promising therapeutic molecule since it reduces and prevents Tau aggregation. This evidence establishes the CST cell-based aggregation assay as a reliable tool for drug discovery and suggests that PD-901 might be a promising compound to be tested for further preclinical studies on AD.
- Published
- 2019
9. Modulation of Tau Subcellular Localization as a Tool to Investigate the Expression of Disease-related Genes
- Author
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Maria Claudia Caiazza, Valentina Quercioli, Cristina Di Primio, Giacomo Siano, Antonino Cattaneo, Mariantonietta Calvello, Martina Varisco, Siano, G., Caiazza, M. C., Varisco, M., Calvello, M., Quercioli, V., Cattaneo, A., and Di Primio, C.
- Subjects
Nucleolus ,General Chemical Engineering ,Cellular differentiation ,Tau protein ,tau Proteins ,Biology ,General Biochemistry, Genetics and Molecular Biology ,03 medical and health sciences ,0302 clinical medicine ,Alzheimer Disease ,Cell Line, Tumor ,Humans ,Gene ,030304 developmental biology ,Cell Nucleus ,Neurons ,Regulation of gene expression ,0303 health sciences ,General Immunology and Microbiology ,General Neuroscience ,Binding protein ,Cell Differentiation ,Subcellular localization ,RRNA transcription ,Cell biology ,Gene Expression Regulation ,Vesicular Glutamate Transport Protein 1 ,biology.protein ,030217 neurology & neurosurgery ,Subcellular Fractions - Abstract
Tau is a microtubule binding protein expressed in neurons and its main known function is related to the maintenance of cytoskeletal stability. However, recent evidence indicated that Tau is present also in other subcellular compartments including the nucleus where it is implicated in DNA protection, in rRNA transcription, in the mobility of retrotransposons and in the structural organization of the nucleolus. We have recently demonstrated that nuclear Tau is involved in the expression of the VGluT1 gene, suggesting a molecular mechanism that could explain the pathological increase of glutamate release in the early stages of Alzheimer's disease. Until recently, the involvement of nuclear Tau in modulating the expression of target genes has been relatively uncertain and ambiguous due to technical limitations that prevented the exclusion of the contribution of cytoplasmic Tau or the effect of other downstream factors not related to nuclear Tau. To overcome this uncertainty, we developed a method to study the expression of target genes specifically modulated by the nuclear Tau protein. We employed a protocol that couples the use of localization signals and the subcellular fractionation, allowing the exclusion of the interference from the cytoplasmic Tau molecules. Most notably, the protocol is easy and is composed of classic and reliable methods that are broadly applicable to study the nuclear function of Tau in other cell types and cellular conditions.
- Published
- 2019
10. Tau-dependent HDAC1 nuclear reduction is associated with altered VGluT1 expression.
- Author
-
Siano G, Madaro G, Caiazza MC, Allouch A, Varisco M, Mignanelli M, Cattaneo A, and Di Primio C
- Abstract
During AD pathology, Tau protein levels progressively increase from early pathological stages. Tau altered expression causes an unbalance of Tau subcellular localization in the cytosol and in the nuclear compartment leading to synaptic dysfunction, neuronal cell death and neurodegeneration as a consequence. Due to the relevant role of epigenetic remodellers in synaptic activity in physiology and in neurodegeneration, in particular of TRIM28 and HDAC1, we investigated the relationship between Tau and these epigenetic factors. By molecular, imaging and biochemical approaches, here we demonstrate that Tau altered expression in the neuronal cell line SH-SY5y does not alter TRIM28 and HDAC1 expression but it induces a subcellular reduction of HDAC1 in the nuclear compartment. Remarkably, HDAC1 reduced activity modulates the expression of synaptic genes in a way comparable to that observed by Tau increased levels. These results support a competitive relationship between Tau levels and HDAC1 subcellular localization and nuclear activity, indicating a possible mechanism mediating the alternative role of Tau in the pathological alteration of synaptic genes expression., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2023 Siano, Madaro, Caiazza, Allouch, Varisco, Mignanelli, Cattaneo and Di Primio.)
- Published
- 2023
- Full Text
- View/download PDF
11. Crystal structure of di-μ-chlorido-bis-{chlorido-[(-)-5,6-pinenebi-pyridine]-cobalt(II)} aqua-dichlorido[(-)-5,6-pinenebi-pyridine]cobalt(II).
- Author
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Varisco M, Crochet A, and Mamula Steiner O
- Abstract
The crystal structure of [Co
2 Cl4 (C17 H18 N2 )2 ][CoCl2 (C17 H18 N2 )(H2 O)] or [Co( L )Cl(μ-Cl)]2 [Co( L )(Cl)2 (OH2 )], where L is the enanti-opure bidentate ligand (-)-5,6-pinenebi-pyridine (C17 H18 N2 ), has been determined. Crystals suitable for X-ray structure analysis were obtained by slow evaporation of an ethano-lic solution containing equimolar amounts of L and CoCl2 ·6H2 O. The CoII cations all have a coordination number of five, and in each case the coordination polyhedron is a trigonal bipyramid. The Co-N bonds lengths range from 2.037 (7) to 2.195 (7) Å, and Co-Cl bonds lengths range from 2.284 (2) to 2.509 (2) Å. The asymmetric unit contains two discrete complexes, one dinuclear and the other mononuclear. Between the two mol-ecules, two types of inter-molecular inter-actions have been evidenced: π-π stackings involving the bi-pyridine units, and O-H⋯Cl hydrogen bonds between the hydrogen atoms of the aqua ligand coordinating to the mononuclear complex and the non-bridging chlorido ligand coordinating to the dinuclear mol-ecule. These inter-actions lead to a two-dimensional supra-molecular arrangement parallel to the ab plane., (© Varisco et al. 2022.)- Published
- 2022
- Full Text
- View/download PDF
12. Jet Colliding and Mixing Efficiency.
- Author
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De Lucia G, Varisco M, Eastes RE, and Allemann C
- Subjects
- Research Design
- Abstract
Two experimental methods, the Nile Red dye extraction and the Williamson ether synthesis in biphasic conditions, were used to characterize the mixing performance of a new cheap impinging jet colliding mixer from Gjosa and to compare it to other commercial micromixers (Caterpillar CPMM-R300, T-mixer, LTF MR-MX and LTF MR-MS). The Nile Red method shows that the Caterpillar mixer is the best one. Excellent results are also achieved with two Gjosa mixers in series. These results are not reflected in the Williamson ether synthesis, where the best mixer is the Gjosa one.
- Published
- 2021
- Full Text
- View/download PDF
13. Electromyographic Evaluation of Masticatory Muscles in a Young Patient with Crossbite Treated with Rapid Palatal Expander: A Case Report.
- Author
-
Maddalone M, Nanussi A, Varisco M, Cortese M, Scali J, and Bianco E
- Subjects
- Child, Electromyography, Humans, Male, Masseter Muscle, Masticatory Muscles, Reproducibility of Results, Temporal Muscle, Malocclusion therapy, Palatal Expansion Technique
- Abstract
Aim and Objective: The purpose of this work is to investigate by electromyographic (EMG) surface analysis whether and how the variations in the occlusion due to the correction of the posterior crossbite using a rapid palatal expander (RPE) is accompanied by changes in the activity of the elevator muscles in the pediatric patients., Background: Posterior crossbite is a disgnathic jaw relationship common in patients undergoing growth. In the last 10 years, several studies demonstrated the effectiveness and reproducibility of surface EMG in the objective evaluation of temporal muscle and masseter activity and how this evidence can be an interesting aid in dental clinical practice., Case Description: The case subject BF is an 11-year-old boy with a right I class relationship and a left II class relationship treated with a rapid palatal expansion protocol with a turn of the transversal screw twice a day (0.25 mm each turn) for a week. In this case report, EMG was used to evaluate the temporal muscle and masseter activity immediately before, immediately after, and 4 months after the RPE protocol., Conclusion: Four months after the treatment, a condition of well-being and neuromuscular equilibrium such as that of starting was preserved., Clinical Significance: Electromyography is a noninvasive exam which evaluates the masticatory muscle activity by facial application of electrodes on masseter and temporal muscles. It can be an interesting aid in orthodontic clinical practice to evaluate preservation of a good muscular balance following orthodontic and orthognathic movements.
- Published
- 2020
14. Identification of an ERK Inhibitor as a Therapeutic Drug Against Tau Aggregation in a New Cell-Based Assay.
- Author
-
Siano G, Caiazza MC, Ollà I, Varisco M, Madaro G, Quercioli V, Calvello M, Cattaneo A, and Di Primio C
- Abstract
Formation of Tau aggregates is a common pathological feature of tauopathies and their accumulation directly correlates with cytotoxicity and neuronal degeneration. Great efforts have been made to understand Tau aggregation and to find therapeutics halting or reversing the process, however, progress has been slowed due to the lack of a suitable method for monitoring Tau aggregation. We developed a cell-based assay allowing to detect and quantify Tau aggregation in living cells. The system is based on the FRET biosensor CST able to monitor the molecular dynamic of Tau aggregation in different cellular conditions. We probed candidate compounds that could block Tau hyperphosphorylation. In particular, to foster the drug discovery process, we tested kinase inhibitors approved for the treatment of other diseases. We identified the ERK inhibitor PD-901 as a promising therapeutic molecule since it reduces and prevents Tau aggregation. This evidence establishes the CST cell-based aggregation assay as a reliable tool for drug discovery and suggests that PD-901 might be a promising compound to be tested for further preclinical studies on AD.
- Published
- 2019
- Full Text
- View/download PDF
15. Synthesis of hydrophilic and hydrophobic carbon quantum dots from waste of wine fermentation.
- Author
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Varisco M, Zufferey D, Ruggi A, Zhang Y, Erni R, and Mamula O
- Abstract
Wine lees are one of the main residues formed in vast quantities during the fermentation of wine. While toxic when applied to plants and wetlands, it is a biodegradable material, and several alternatives have been proposed for its valorization as: dietary supplement in animal feed, source for various yeast extracts and bioconversion feedstock. The implementation of stricter environment protection regulations resulted in increasing costs for wineries as their treatment process constitutes an unavoidable and expensive step in wine production. We propose here an alternative method to reduce waste and add value to wine production by exploiting this rich carbon source and use it as a raw material for producing carbon quantum dots (CQDs). A complete synthetic pathway is discussed, comprising the carbonization of the starting material, the screening of the most suitable solvent for the extraction of CQDs from the carbonized mass and their hydrophobic or hydrophilic functionalization. CQDs synthesized with the reported procedure show a bright blue emission ( λ
max = 433 ± 13 nm) when irradiated at 366 nm, which is strongly shifted when the wavelength is increased (e.g. emission at around 515 nm when excited at 460 nm). Yields and luminescent properties of CQDs, obtained with two different methods, namely microwave and ultrasound-based extraction, are discussed and compared. This study shows how easy a residue can be converted into an added-value material, thus not only reducing waste and saving costs for the wine-manufacturing industry but also providing a reliable, affordable and sustainable source for valuable materials., Competing Interests: The authors declare no competing interests.- Published
- 2017
- Full Text
- View/download PDF
16. Preventing Implant-Associated Infections by Silver Coating.
- Author
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Kuehl R, Brunetto PS, Woischnig AK, Varisco M, Rajacic Z, Vosbeck J, Terracciano L, Fromm KM, and Khanna N
- Subjects
- Animals, Anti-Bacterial Agents pharmacokinetics, Cell Survival drug effects, Daptomycin pharmacology, Drug Liberation, Female, Kinetics, Leukocytes drug effects, Methicillin-Resistant Staphylococcus aureus drug effects, Methicillin-Resistant Staphylococcus aureus growth & development, Mice, Mice, Inbred C57BL, Microbial Sensitivity Tests, Silver pharmacokinetics, Skin, Staphylococcus epidermidis drug effects, Staphylococcus epidermidis growth & development, Vancomycin pharmacology, Anti-Bacterial Agents pharmacology, Coated Materials, Biocompatible pharmacology, Dental Alloys pharmacology, Prostheses and Implants microbiology, Silver pharmacology, Staphylococcal Infections prevention & control
- Abstract
Implant-associated infections (IAIs) are a dreaded complication mainly caused by biofilm-forming staphylococci. Implant surfaces preventing microbial colonization would be desirable. We examined the preventive effect of a silver-coated titanium-aluminum-niobium (TiAlNb) alloy. The surface elicited a strong, inoculum-dependent activity againstStaphylococcus epidermidisandStaphylococcus aureusin an agar inhibition assay. Gamma sterilization and alcohol disinfection did not alter the effect. In a tissue cage mouse model, silver coating of TiAlNb cages prevented perioperative infections in an inoculum-dependent manner and led to a 100% prevention rate after challenge with 2 × 10(6)CFU ofS. epidermidisper cage. InS. aureusinfections, silver coating had only limited effect. Similarly, daptomycin or vancomycin prophylaxis alone did not preventS. aureusinfections. However, silver coating combined with daptomycin or vancomycin prophylaxis thwarted methicillin-resistantS. aureusinfections at a prevention rate of 100% or 33%, respectively. Moreover, silver release from the surface was independent of infection and occurred rapidly after implantation. On day 2, a peak of 82 μg Ag/ml was reached in the cage fluid, corresponding to almost 6× the MIC of the staphylococci. Cytotoxicity toward leukocytes in the cage was low and temporary. Surrounding tissue did not reveal histological signs of silver toxicity.In vitro, no emergence of silver resistance was observed in several clinical strains of staphylococci upon serial subinhibitory silver exposures. In conclusion, our data demonstrate that silver-coated TiAlNb is potent for prevention of IAIs and thus can be considered for clinical application., (Copyright © 2016, American Society for Microbiology. All Rights Reserved.)
- Published
- 2016
- Full Text
- View/download PDF
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