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One of the most devastating adverse effects of cancer treatments including bone marrow transplantation is damage to the reproductive system, which in young girls and women less than 40 years old is frequently associated with premature menopause and infertility, although adult bone marrow stem cells (BMSCs) are able to generate cells of all three germ layers under appropriate experimental conditions. These outcomes seem to be a result of cytotoxic effects on oocytes housed within the ovaries. Recent studies showed that female BMSCs (FBMSC) could differentiate into granulosa cells (GCs) and oocyte and form primordial follicles in ovary after FBMSCs transplantation and might have repaired chemotherapy-induced damage to the niche or the ovarian stroma. The formation of new primordial follicles in adult mammal ovaries is a controversial issue because some new studies have concluded that all offspring were derived from the recipient germ line after FBMSCs transplantation. To explore the effects of FBMSCs on female reproductive function, the experiment is to investigate whether adult female BMSCs are able to differentiate into Follicle-stimulating hormone receptor (FSHR, a GCs specific marker in the ovary cells) positive GCs and stra8(stimulated by retinoic acid gene 8, a specific expression gene in mammalian germ cell's transition from mitosis to meiosis) expression oogonia in vitroinduced by all-trans retinoic acid (RA).