Search

Your search keyword '"Yadav, Bhawna"' showing total 23 results
Start Over Author "Yadav, Bhawna" Search Limiters Available in Library Collection
23 results on '"Yadav, Bhawna"'

13. Gene Essentiality Analyzed by In Vivo Transposon Mutagenesis and Machine Learning in a Stable Haploid Isolate of Candida albicans

Author

Segal, Ella Shtifman, primary, Gritsenko, Vladimir, additional, Levitan, Anton, additional, Yadav, Bhawna, additional, Dror, Naama, additional, Steenwyk, Jacob L., additional, Silberberg, Yael, additional, Mielich, Kevin, additional, Rokas, Antonis, additional, Gow, Neil A. R., additional, Kunze, Reinhard, additional, Sharan, Roded, additional, and Berman, Judith, additional

Published
2018
Full Text
View/download PDF

20. Constitutive expression of SMAR1 confers susceptibility to Mycobacterium tuberculosis infection in a transgenic mouse model.

Author

Yadav, Bhawna, Malonia, Sunil K., Majumdar, Subeer S., Gupta, Pushpa, Wadhwa, Neerja, Badhwar, Archana, Gupta, Umesh D., Katoch, Vishwa M., and Chattopadhyay, Samit

Subjects
*MYCOBACTERIUM tuberculosis, *MYCOBACTERIUM, *TUBERCULIN, *TUBERCULOSIS, *TRANSGENIC mice
Abstract

Background & objectives: Studies involving animal models of experimental tuberculosis have elucidated the predominant role of cytokines secreted by T cells and macrophages to be an essential component of the immune response against Mycobacterium tuberculosis infection. The immune activities of CD4+ T cells are mediated in part by Th1 cytokine interferon gamma (IFN-γ) which is produced primarily by T cells and natural killer (NK) cells and critical for initiating the immune response against intracellular pathogen such as M. tuberculosis. Nuclear matrix protein SMAR1 plays an important role in V(D)J recombination, T helper cell differentiation and inflammatory diseases. In this study a transgenic mouse model was used to study the role of SMAR1 in M. tuberculosis infection. Methods: Wild type BALB/c, C57BL/6, BALB/c-EGFP-SMAR1 and C57BL/6-SMAR1 transgenic mice were infected with M. tuberculosis (H37Rv). A dose of 100 bacilli was used for infection via respiratory route. Bacterial load in lung and spleen of infected mice was determined at 2, 4, 6 and 8 wk post-infection. Gene expression analysis for Th1 cytokines and inducible nitric oxide synthase (iNOS) was performed in infected lung tissues by quantitative reverse transcription (RT)-PCR. Results: SMAR1 transgenic mice from both BALB/c and C57BL/6 genetic background displayed higher bacillary load and susceptibility to M. tuberculosis infection compared to wild type mice. This susceptibility was attributed due to compromised of Th1 response exhibited by transgenic mice. Interpretation & conclusions: SMAR1 transgenic mice exhibited susceptibility to M. tuberculosis infection in vivo irrespective of genetic background. This susceptibility was attributed to downregulation of Th1 response and its hallmark cytokine IFN-γ. Hence, SMAR1 plays an important role in modulating host immune response after M. tuberculosis infection. [ABSTRACT FROM AUTHOR]

Published
2015
Full Text
View/download PDF

23. Gene Essentiality Analyzed by In VivoTransposon Mutagenesis and Machine Learning in a Stable Haploid Isolate of Candida albicans

Author

Segal, Ella Shtifman, Gritsenko, Vladimir, Levitan, Anton, Yadav, Bhawna, Dror, Naama, Steenwyk, Jacob L., Silberberg, Yael, Mielich, Kevin, Rokas, Antonis, Gow, Neil A. R., Kunze, Reinhard, Sharan, Roded, and Berman, Judith

Abstract

Comprehensive understanding of an organism requires that we understand the contributions of most, if not all, of its genes. Classical genetic approaches to this issue have involved systematic deletion of each gene in the genome, with comprehensive sets of mutants available only for very-well-studied model organisms. We took a different approach, harnessing the power of in vivotransposition coupled with deep sequencing to identify >500,000 different mutations, one per cell, in the prevalent human fungal pathogen Candida albicansand to map their positions across the genome. The transposition approach is efficient and less labor-intensive than classic approaches. Here, we describe the production and analysis (aided by machine learning) of a large collection of mutants and the comprehensive identification of 1,610 C. albicansgenes that are essential for growth under standard laboratory conditions. Among these C. albicansessential genes, we identify those that are also essential in two distantly related model yeasts as well as those that are conserved in all four major human fungal pathogens and that are not conserved in the human genome. This list of genes with functions important for the survival of the pathogen provides a good starting point for the development of new antifungal drugs, which are greatly needed because of the emergence of fungal pathogens with elevated resistance and/or tolerance of the currently limited set of available antifungal drugs.

Published
2018
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results