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25 results on '"Yang, Shun-Kai"'

3. Cryo electron tomography with volta phase plate reveals novel structural foundations of the 96-nm axonemal repeat in the pathogen Trypanosoma brucei.

Author

Imhof, Simon, Zhang, Jiayan, Wang, Hui, Bui, Khanh Huy, Nguyen, Hoangkim, Atanasov, Ivo, Hui, Wong H, Yang, Shun Kai, Zhou, Z Hong, and Hill, Kent L

Subjects
Trypanosoma brucei brucei, Protozoan Proteins, Imaging, Three-Dimensional, Cryoelectron Microscopy, Protein Binding, Axoneme, Electron Microscope Tomography, axoneme, cell biology, cilium, dynein, infectious disease, microbiology, motility, parasite, trypanosome, Vector-Borne Diseases, Infectious Diseases, 2.2 Factors relating to the physical environment, Infection, Biochemistry and Cell Biology
Abstract

The 96-nm axonemal repeat includes dynein motors and accessory structures as the foundation for motility of eukaryotic flagella and cilia. However, high-resolution 3D axoneme structures are unavailable for organisms among the Excavates, which include pathogens of medical and economic importance. Here we report cryo electron tomography structures of the 96-nm repeat from Trypanosoma brucei, a protozoan parasite in the Excavate lineage that causes African trypanosomiasis. We examined bloodstream and procyclic life cycle stages, and a knockdown lacking DRC11/CMF22 of the nexin dynein regulatory complex (NDRC). Sub-tomogram averaging yields a resolution of 21.8 Å for the 96-nm repeat. We discovered several lineage-specific structures, including novel inter-doublet linkages and microtubule inner proteins (MIPs). We establish that DRC11/CMF22 is required for the NDRC proximal lobe that binds the adjacent doublet microtubule. We propose that lineage-specific elaboration of axoneme structure in T. brucei reflects adaptations to support unique motility needs in diverse host environments.

Published
2019

16. The inner junction complex of the cilia is an interaction hub that involves tubulin post-translational modifications

Author

Khalifa, Ahmad Abdelzaher Zaki, primary, Ichikawa, Muneyoshi, additional, Dai, Daniel, additional, Kubo, Shintaroh, additional, Black, Corbin Steven, additional, Peri, Katya, additional, McAlear, Thomas S, additional, Veyron, Simon, additional, Yang, Shun Kai, additional, Vargas, Javier, additional, Bechstedt, Susanne, additional, Trempe, Jean-François, additional, and Bui, Khanh Huy, additional

Published
2020
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24. Performance Enhancement in N 2 Plasma Modified AlGaN/AlN/GaN MOS-HEMT Using HfAlO X Gate Dielectric with Γ-Shaped Gate Engineering.

Author

Yang, Shun-Kai, Mazumder, Soumen, Wu, Zhan-Gao, and Wang, Yeong-Her

Subjects
*GALLIUM nitride, *MODULATION-doped field-effect transistors, *PASSIVATION, *METAL oxide semiconductors, *DIELECTRICS, *SURFACE preparation
Abstract

In this paper, we have demonstrated the optimized device performance in the Γ-shaped gate AlGaN/AlN/GaN metal oxide semiconductor high electron mobility transistor (MOS-HEMT) by incorporating aluminum into atomic layer deposited (ALD) HfO2 and comparing it with the commonly used HfO2 gate dielectric with the N2 surface plasma treatment. The inclusion of Al in the HfO2 increased the crystalline temperature (~1000 °C) of hafnium aluminate (HfAlOX) and kept the material in the amorphous stage even at very high annealing temperature (>800 °C), which subsequently improved the device performance. The gate leakage current (IG) was significantly reduced with the increasing post deposition annealing (PDA) temperature from 300 to 600 °C in HfAlOX-based MOS-HEMT, compared to the HfO2-based device. In comparison with HfO2 gate dielectric, the interface state density (Dit) can be reduced significantly using HfAlOX due to the effective passivation of the dangling bond. The greater band offset of the HfAlOX than HfO2 reduces the tunneling current through the gate dielectric at room temperature (RT), which resulted in the lower IG in Γ-gate HfAlOX MOS-HEMT. Moreover, IG was reduced more than one order of magnitude in HfAlOX MOS-HEMT by the N2 surface plasma treatment, due to reduction of N2 vacancies which were created by ICP dry etching. The N2 plasma treated Γ-shaped gate HfAlOX-based MOS-HEMT exhibited a decent performance with IDMAX of 870 mA/mm, GMMAX of 118 mS/mm, threshold voltage (VTH) of −3.55 V, higher ION/IOFF ratio of approximately 1.8 × 109, subthreshold slope (SS) of 90 mV/dec, and a high VBR of 195 V with reduced gate leakage current of 1.3 × 10−10 A/mm. [ABSTRACT FROM AUTHOR]

Published
2021
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25. Transcriptomic analysis of multi-drug resistant Escherichia coliK-12 strain in response to Lavandula angustifoliaessential oil

Author

Lai, Pey-Jiun, Ng, Ee-Von, Yang, Shun-Kai, Moo, Chew-Li, Low, Wai Yee, Yap, Polly Soo-Xi, Lim, Swee-Hua Erin, and Lai, Kok-Song

Abstract

To better understand the synergistic antibacterial activity between piperacillin and Lavandula angustifoliaessential oil (LEO) against multidrug-resistant Escherichia coli, we performed microarray transcriptomic analysis of LEO when used alone and in combination with piperacillin against the non-treated control. In total, 90 genes were differentially expressed after the combination of LEO and piperacillin treatment. Among the up-regulated genes, nfsB, nemA, fruA, nfsB, nemAare known to control microbial metabolism and nitrotoluene degradation, which were observed only in the LEO–piperacillin combinatory treatment. Four candidate genes from the microarray result, srIA, srID, waaR and nfsB,were validated by qRT-PCR as these genes showed differential expression consistently in the two methods. Biochemical pathway analysis showed that there was upregulation of genes involved in several biological processes including fructose and mannose metabolism, phosphotransferase system (PTS), lipopolysaccharide biosynthesis and nitrotoluene degradation. Genes involved in microbial metabolism in diverse environments were found both up- and down-regulated in LEO–piperacillin combinatory treatment. Our study provides new information concerning the transcriptional changes that occur during the LEO and piperacillin interaction against the multidrug-resistant bacteria and contributes to unravel the mechanisms underlying this synergism.

Published
2020
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