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4. Synthesis and calcination–temperature-dependent gas-sensing performance of g-C3N4/Co3O4 heterojunctions for toluene gas sensors.

Author

Yue, J. H., Xu, J. C., Hong, B., Li, J., Zeng, Y. X., Gong, J., Peng, X. L., Ge, H. L., Chen, H. W., and Wang, X. Q.

Subjects
GAS detectors, P-N heterojunctions, HETEROJUNCTIONS, CALCINATION (Heat treatment), TOLUENE, SURFACE area, NANOSTRUCTURED materials
Abstract

G-C3N4 nanosheets were synthesized by hydrothermal method and then were anchored on the surface of mesoporous Co3O4 nanowires (NWs) to from g-C3N4/Co3O4 heterojunctions. After calcination, the influence of calcined temperature on the microstructures and gas-sensing performance is investigated in detail. All results show that g-C3N4 nanosheets affect the microstructure of Co3O4 NWs and are decorated on the surface of Co3O4 NWs. With the increasing calcination temperature, the specific surface area decreased from 64 m2/g for Co3O4 NWs to about 20 m2/g. The responses of g-C3N4/Co3O4 sensors are improved from 11.01 for Co3O4 sensor to about 20–100 ppm toluene gas, and CNC-500 presents excellent response values of 25.8 at the operating temperature of 220 °C. Although g-C3N4/Co3O4 heterojunctions exhibit the low specific surface area, the p-n heterojunctions at the interface of g-C3N4 and Co3O4 greatly increase the resistance in toluene gas. As the result, g-C3N4 nanosheets greatly improve the toluene gas-sensing performance of g-C3N4/Co3O4 sensors due to p-n heterojunctions. [ABSTRACT FROM AUTHOR]

Published
2023
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7. Synthesis and enhanced formaldehyde gas-sensing performance of mesoporous SnO2 nanospheres doped with trivalent metal-cations.

Author

Zhou, F. Y., Hong, B., Xu, J. C., Han, Y. B., Jin, H. X., Jin, D. F., Zeng, Y. X., Peng, X. L., Ge, H. L., and Wang, X. Q.

Subjects
CHEMICAL synthesis, FORMALDEHYDE, SURFACE area, CRYSTAL structure, DOPING agents (Chemistry), POROUS metals
Abstract

Mesoporous SnO2, Sn0.861Al0.186O2, Sn0.921In0.106O2 and Sn0.959La0.055O2 nanospheres were successfully synthesized by a one-step solvothermal method. The compositions, crystal structures, microstructures, morphology and gas-sensing performance of the as-synthesized Al-, In- and La-doped SnO2 nanospheres were characterized by XRD, EDS, TEM, SEM, FT-IR, UV–vis and BET. All samples present the similar mesoporous-structural nanospheres due to the same chemical synthesis conditions. The gas-sensing results indicate that the cations-doping greatly affects the formaldehyde gas-sensing performance, and the response increases with the increasing radius of Al, In and La. Owing to the higher specific surface area and larger La3+ radius, Sn0.959La0.055O2 sensor presents the best gas-sensing performance with the response value of 149.59 to formaldehyde gas at 200 °C. It is concluded that metal-cation doping not only causes lattice distortion to increase oxygen vacancies, but also could refine the crystalline grain to improve the specific surface area of metal-cations doped SnO2 nanospheres. [ABSTRACT FROM AUTHOR]

Published
2023
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20. Need for global action for cancer control

Author

Boyle, P., Anderson, B. O., Andersson, L. C., Ariyaratne, Y., Auleley, G.-R., Barbacid, M., Bartelink, H., Baselga, J., Behbehani, K., Belardelli, F., Berns, A., Bishop, J., Brawley, O., Burns, H., Clanton, M., Cox, B., Currow, D., Dangou, J.-M., de Valeriola, D., Dinshaw, K., Eggermont, A., Fitzpatrick, J., Forstmane, M., Garaci, E., Gavin, A. T., Kakizoe, T., Kasler, M., Keita, N., Kerr, D., Khayat, D., Khleif, S., Khuhaprema, T., Knezevic, T., Kubinova, R., Mallath, M., Martin-Moreno, J., McCance, D., McVie, J. G., Merriman, A., Ngoma, T., Nowacki, M., Orgelbrand, J., Park, J.-G., Pierotti, M., Ashton, L. P., Puska, P., Escobar, C. V. R., Rajan, B., Rajkumar, T., Ringborg, U., Robertson, C., Rodger, A., Roovali, L., Santini, L. A., Sarhan, M., Seffrin, J., Semiglazov, V., Shrestha, B. M., Soo, K. C., Stamenic, V., Tamblyn, C., Thomas, R., Tuncer, M., Tursz, T., Vaitkiene, R., Vallejos, C., Veronesi, U., Wojtyla, A., Yach, D., Yoo, K.-Y., Zatonski, W., Zaridze, D., Zeng, Y.-X., Zhao, P., and Zheng, T.

Published
2008

21. BDNF activates TrkB/PLCγ1 signaling pathway to promote proliferation and invasion of ovarian cancer cells through inhibition of apoptosis

Author

Xu, Y., Jiang, W. -G., Wang, H. -C., Martin, T., Zeng, Y. -X., Zhang, J., and Qi, Y. -S.

Subjects
nervous system, embryonic structures
Abstract

OBJECTIVE: Abnormal expression and activation of tropomyosin-related kinase receptor B (TrkB) are observed in many pathological conditions, including many types of cancer. We try to explore the relationship between ovarian cancer and Brain-derived neurotrophic factor (BDNF), a ligand of TrkB. MATERIALS AND METHODS: Human ovarian cancer cell line SKOV-3 was used in this study. qPCR, immunohistochemistry, and immunoblot were used to assay BDNF and TrkB expression level. Scratch assay was used to test the cell motility, and transwell assay was used to test the cell migration ability. RESULTS: We found that BDNF promotes the proliferation and invasion of human ovarian cancer SKOV-3 cells depend on the activation of TrkB. To illuminate the downstream pathway of BDNF/TrkB, we silenced AKT1 and PLCγ1 by siRNA. The functional assay showed that activated PLCγ1 signaling pathway is necessary for the proliferation and invasion of cancer cells other than the AKT pathway. Further study showed that PLCγ1 could inhibit the apoptosis of cancer cells. CONCLUSIONS: BDNF triggers TrkB/PLCγ1 signaling pathway to promote proliferation and invasion of ovarian cancer cells through inhibition of apoptosis.

Published
2019

22. Novel Common Genetic Susceptibility Loci for Colorectal Cancer

Author

Schmit, SL, Edlund, CK, Schumacher, FR, Gong, J, Harrison, TA, Huyghe, JR, Qu, C, Melas, M, Van den Berg, DJ, Wang, H, Tring, S, Plummer, SJ, Albanes, D, Alonso, MH, Amos, CI, Anton, K, Aragaki, AK, Arndt, V, Barry, EL, Berndt, SI, Bezieau, S, Bien, S, Bloomer, A, Boehm, J, Boutron-Ruault, M-C, Brenner, H, Brezina, S, Buchanan, DD, Butterbach, K, Caan, BJ, Campbell, PT, Carlson, CS, Castelao, JE, Chan, AT, Chang-Claude, J, Chanock, SJ, Cheng, I, Cheng, Y-W, Chin, LS, Church, JM, Church, T, Coetzee, GA, Cotterchio, M, Correa, MC, Curtis, KR, Duggan, D, Easton, DF, English, D, Feskens, EJM, Fischer, R, FitzGerald, LM, Fortini, BK, Fritsche, LG, Fuchs, CS, Gago-Dominguez, M, Gala, M, Gallinger, SJ, Gauderman, WJ, Giles, GG, Giovannucci, EL, Gogarten, SM, Gonzalez-Villalpando, C, Gonzalez-Villalpando, EM, Grady, WM, Greenson, JK, Gsur, A, Gunter, M, Haiman, CA, Hampe, J, Harlid, S, Harju, JF, Hayes, RB, Hofer, P, Hoffmeister, M, Hopper, JL, Huang, S-C, Huerta, JM, Hudson, TJ, Hunter, DJ, Idos, GE, Iwasaki, M, Jackson, RD, Jacobs, EJ, Jee, SH, Jenkins, MA, Jia, W-H, Jiao, S, Joshi, AD, Kolonel, LN, Kono, S, Kooperberg, C, Krogh, V, Kuehn, T, Kury, S, LaCroix, A, Laurie, CA, Lejbkowicz, F, Lemire, M, Lenz, H-J, Levine, D, Li, CI, Li, L, Lieb, W, Lin, Y, Lindor, NM, Liu, Y-R, Loupakis, F, Lu, Y, Luh, F, Ma, J, Mancao, C, Manion, FJ, Markowitz, SD, Martin, V, Matsuda, K, Matsuo, K, McDonnell, KJ, McNeil, CE, Milne, R, Molina, AJ, Mukherjee, B, Murphy, N, Newcomb, PA, Offit, K, Omichessan, H, Palli, D, Cotore, JPP, Perez-Mayoral, J, Pharoah, PD, Potter, JD, Raskin, L, Rennert, G, Rennert, HS, Riggs, BM, Schafmayer, C, Schoen, RE, Sellers, TA, Seminara, D, Severi, G, Shi, W, Shibata, D, Shu, X-O, Siegel, EM, Slattery, ML, Southey, M, Stadler, ZK, Stern, MC, Stintzing, S, Taverna, D, Thibodeau, SN, Thomas, DC, Trichopoulou, A, Tsugane, S, Ulrich, CM, van Duijnhoven, FJB, van Guelpan, B, Vijai, J, Virtamo, J, Weinstein, SJ, White, E, Win, AK, Wolk, A, Woods, M, Wu, AH, Wu, K, Xiang, Y-B, Yen, Y, Zanke, BW, Zeng, Y-X, Zhang, B, Zubair, N, Kweon, S-S, Figueiredo, JC, Zheng, W, Le Marchand, L, Lindblom, A, Moreno, V, Peters, U, Casey, G, Hsu, L, Conti, DV, Gruber, SB, Schmit, SL, Edlund, CK, Schumacher, FR, Gong, J, Harrison, TA, Huyghe, JR, Qu, C, Melas, M, Van den Berg, DJ, Wang, H, Tring, S, Plummer, SJ, Albanes, D, Alonso, MH, Amos, CI, Anton, K, Aragaki, AK, Arndt, V, Barry, EL, Berndt, SI, Bezieau, S, Bien, S, Bloomer, A, Boehm, J, Boutron-Ruault, M-C, Brenner, H, Brezina, S, Buchanan, DD, Butterbach, K, Caan, BJ, Campbell, PT, Carlson, CS, Castelao, JE, Chan, AT, Chang-Claude, J, Chanock, SJ, Cheng, I, Cheng, Y-W, Chin, LS, Church, JM, Church, T, Coetzee, GA, Cotterchio, M, Correa, MC, Curtis, KR, Duggan, D, Easton, DF, English, D, Feskens, EJM, Fischer, R, FitzGerald, LM, Fortini, BK, Fritsche, LG, Fuchs, CS, Gago-Dominguez, M, Gala, M, Gallinger, SJ, Gauderman, WJ, Giles, GG, Giovannucci, EL, Gogarten, SM, Gonzalez-Villalpando, C, Gonzalez-Villalpando, EM, Grady, WM, Greenson, JK, Gsur, A, Gunter, M, Haiman, CA, Hampe, J, Harlid, S, Harju, JF, Hayes, RB, Hofer, P, Hoffmeister, M, Hopper, JL, Huang, S-C, Huerta, JM, Hudson, TJ, Hunter, DJ, Idos, GE, Iwasaki, M, Jackson, RD, Jacobs, EJ, Jee, SH, Jenkins, MA, Jia, W-H, Jiao, S, Joshi, AD, Kolonel, LN, Kono, S, Kooperberg, C, Krogh, V, Kuehn, T, Kury, S, LaCroix, A, Laurie, CA, Lejbkowicz, F, Lemire, M, Lenz, H-J, Levine, D, Li, CI, Li, L, Lieb, W, Lin, Y, Lindor, NM, Liu, Y-R, Loupakis, F, Lu, Y, Luh, F, Ma, J, Mancao, C, Manion, FJ, Markowitz, SD, Martin, V, Matsuda, K, Matsuo, K, McDonnell, KJ, McNeil, CE, Milne, R, Molina, AJ, Mukherjee, B, Murphy, N, Newcomb, PA, Offit, K, Omichessan, H, Palli, D, Cotore, JPP, Perez-Mayoral, J, Pharoah, PD, Potter, JD, Raskin, L, Rennert, G, Rennert, HS, Riggs, BM, Schafmayer, C, Schoen, RE, Sellers, TA, Seminara, D, Severi, G, Shi, W, Shibata, D, Shu, X-O, Siegel, EM, Slattery, ML, Southey, M, Stadler, ZK, Stern, MC, Stintzing, S, Taverna, D, Thibodeau, SN, Thomas, DC, Trichopoulou, A, Tsugane, S, Ulrich, CM, van Duijnhoven, FJB, van Guelpan, B, Vijai, J, Virtamo, J, Weinstein, SJ, White, E, Win, AK, Wolk, A, Woods, M, Wu, AH, Wu, K, Xiang, Y-B, Yen, Y, Zanke, BW, Zeng, Y-X, Zhang, B, Zubair, N, Kweon, S-S, Figueiredo, JC, Zheng, W, Le Marchand, L, Lindblom, A, Moreno, V, Peters, U, Casey, G, Hsu, L, Conti, DV, and Gruber, SB

Abstract

BACKGROUND: Previous genome-wide association studies (GWAS) have identified 42 loci (P < 5 × 10-8) associated with risk of colorectal cancer (CRC). Expanded consortium efforts facilitating the discovery of additional susceptibility loci may capture unexplained familial risk. METHODS: We conducted a GWAS in European descent CRC cases and control subjects using a discovery-replication design, followed by examination of novel findings in a multiethnic sample (cumulative n = 163 315). In the discovery stage (36 948 case subjects/30 864 control subjects), we identified genetic variants with a minor allele frequency of 1% or greater associated with risk of CRC using logistic regression followed by a fixed-effects inverse variance weighted meta-analysis. All novel independent variants reaching genome-wide statistical significance (two-sided P < 5 × 10-8) were tested for replication in separate European ancestry samples (12 952 case subjects/48 383 control subjects). Next, we examined the generalizability of discovered variants in East Asians, African Americans, and Hispanics (12 085 case subjects/22 083 control subjects). Finally, we examined the contributions of novel risk variants to familial relative risk and examined the prediction capabilities of a polygenic risk score. All statistical tests were two-sided. RESULTS: The discovery GWAS identified 11 variants associated with CRC at P < 5 × 10-8, of which nine (at 4q22.2/5p15.33/5p13.1/6p21.31/6p12.1/10q11.23/12q24.21/16q24.1/20q13.13) independently replicated at a P value of less than .05. Multiethnic follow-up supported the generalizability of discovery findings. These results demonstrated a 14.7% increase in familial relative risk explained by common risk alleles from 10.3% (95% confidence interval [CI] = 7.9% to 13.7%; known variants) to 11.9% (95% CI = 9.2% to 15.5%; known and novel variants). A polygenic risk score identified 4.3% of the population at an odds ratio for developing CRC of at least 2.0. CONCLUSIONS: T

Published
2019

23. Proceedings of the 7th Biannual International Symposium on Nasopharyngeal Carcinoma 2015

Author

Tan, IB, Chang, Ellen T., Chen, Chien-Jen, Hsu, Wan-Lun, Chien, Yin-Chu, Hildesheim, Allan, McKay, James D., Gaborieau, Valerie, Kaderi, Mohamed Arifin Bin, Purnomosari, Dewajani, Voegele, Catherine, LeCalvez-Kelm, Florence, Byrnes, Graham, Brennan, Paul, Devi, Beena, Li, L., Zhang, Y., Fan, Y., Sun, K., Du, Z., Sun, H., Chan, A. T., Tsao, S. W., Zeng, Y. X., Tao, Q., Busson, Pierre, Lhuillier, Claire, Morales, Olivier, Mrizak, Dhafer, Gelin, Aurore, Kapetanakis, Nikiforos, Delhem, Nadira, Mansouri, Sheila, Cao, Jennifer, Vaidya, Anup, Frappier, Lori, Wai, Lo Kwok, Chen, Sui-Hong, Du, Jin-lin, Ji, Ming-Fang, Huang, Qi-Hong, Liu, Qing, Cao, Su-Mei, Doolan, Denise L., Coghill, Anna, Mulvenna, Jason, Proietti, Carla, Lekieffre, Lea, Bethony, Jeffrey, Hildesheim, and Allan, Fles, Renske, Indrasari, Sagung Rai, Herdini, Camelia, Martini, Santi, Isfandiari, Atoillah, Rhomdoni, Achmad, Adham, Marlinda, Mayangsari, Ika, van Werkhoven, Erik, Wildeman, Maarten, Hariwiyanto, Bambang, Hermani, Bambang, Kentjono, Widodo Ario, Haryana, Sofia Mubarika, Schmidt, Marjanka, O’Sullivan, Brian, Ozyar, Enis, Lee, Anne W. M., Zeng, Mu-Sheng, Gao, Xiaojiang, Tang, Minzhong, Martin, Pat, Zeng, Yi, Carrington, Mary, Coghill, Anna E., Bu, Wei, Nguyen, Hanh, Yu, Kelly J., Lou, Pei-Jen, Wang, Cheng-Ping, Cohen, Jeffrey I., King, Ann D., Chen, Tseng-Cheng, Lin, Ching-Yuan, Tsou, Yung-An, Leu, Yi-Shing, Laio, Li-Jen, Chang, Yen-Liang, Hua, Chun-Hun, Wu, Ming-Shiang, Hsiao, Chu-Hsing Kate, Lee, Jehn-Chuan, Tsai, Ming-Hsui, Cheng, Skye Hung-Chun, Liao, Li-Jen, Yang, Tsung-Lin, Ko, Jenq-Yuh, Ko, Josephine Mun Yee, Dai, Wei, Kwong, Dora, Ng, Wai Tong, Lee, Anne, Ngan, Roger Kai Cheong, Yau, Chun Chung, Tung, Stewart, Lung, Maria Li, Ji, Mingfang, Sheng, Wei, Ng, Mun Hon, Cheng, Weimin, Yu, Xia, Wu, Biaohua, Wei, Kuangrong, Zhan, Jun, Zeng, Yi Xin, Cao, Su Mei, Xia, Ningshao, Yuan, Yong, Cui, Qian, Xu, Miao, Bei, Jin-Xin, Zeng, Yi-Xin, Şahin, B, Dizman, A, Esassolak, M, İkizler, A Saran, Yıldırım, HC, Çaloğlu, M, Atalar, B, Akman, F, Demiroz, C, Atasoy, BM, Canyilmaz, E, Igdem, S, Ugurluer, G, Kütük, T, Akmansoy, M, Ozyar, E, Sommat, Kiattisa, Wang, Fu Qiang, Kwok, Li-Lian, Tan, Terence, Fong, Kam Weng, Soong, Yoke Lim, Cheah, Shie Lee, Wee, Joseph, Casanova, M, Özyar, E, Patte, C, Orbach, D, Ferrari, A, Cristine, VF, Errihani, H, Pan, J, Zhang, L, Liji, S, Grzegorzewski, K, Gore, L, Varan, A, Hutajulu, Susanna Hilda, Khuzairi, Guntara, Kusumo, Henry, Hardianti, Mardiah Suci, Taroeno-Hariadi, Kartika Widayati, Purwanto, Ibnu, Kurnianda, Johan, Messick, Troy E., Malecka, Kimberly, Tolvinski, Lois, Soldan, Samantha, Deakyne, Julianna, Song, Hui, van den Heuvel, Antonio, Gu, Baiwei, Cassel, Joel, McDonnell, Mark, Smith, Garry R., Velvadapu, Venkata, Bian, Haiyan, Zhang, Yan, Carlsen, Marianne, Chen, Shuai, Donald, Alastair, Lemmen, Christian, Reitz, Allen B., Lieberman, Paul M., Chan, King Chi, Chan, Lai Sheung, Lo, Kwok Wai, Yip, Timothy Tak Chun, Kahn, Michael, Mak, Nai Ki, Liu, Fei-Fei, Khaali, Wafa, Thariat, Juliette, Fantin, Laurence, Spirito, Flavia, Khyatti, Meriem, Driss, El Khalil Ben, Olivero, Sylvain, Maryanski, Janet, Doglio, Alain, Xia, Mengxue, Xia, Yunfei, Chang, Hui, Shaw, Rachel, Rahaju, Pudji, Wisesa, Sindhu, Taroeno-Harijadi, Kartika Widayati, Dhamiyati, Wigati, Tan, Sang-Nee, Sim, Sai-Peng, Yusuf, Muhtarum, Romdhoni, Ahmad C., K, Widodo Ario, Rantam, Fedik Abdul, Sugiyanto, Aryati, Lina, Adi-Kusumo, Fajar, Bintoro, SY, Oktriani, R., Herawati, C., Surono, A., Haryana, Sofia M., Zhong, L., Ma, B. B., Kalra, M., Ngo, M., Perna, S., Leen, A., Lapteva, N., Rooney, C. M., Gottschalk, S., Mustikaningtyas, Elida, Herawati, Sri, Romdhoni, Achmad C., Xu, Yarui, Ge, Shengxiang, Li, Fugui, Ng, M. H., Tan, Louise SY, Wong, Benjamin, Lim, C. M., Rantam, Fedik A., Madani, Deasy Z., Akbar, Nur, Permana, Agung Dinasti, Fachiroh, Jajah, Hartati, Dwi, Rahayudjati, T. Baning, Darwis, Iswandi, Anwar, Khoirul, Dwidanarti, Sri Retna, Pramana, Dominicus Wendhy, Safitri, Diah Ari, Danarti, Sri Retna Dwi, Taroeno, Suryo A, Wijaya, I., Oehadian, A., Prasetya, D., Yu, Kelly J, Rahman, Sukri, Budiman, Bestari J., Novialdi, Rahmadona, Lestari, Dewi Yuri, Yin, C., Foussadier, A., Blein, E., Chen, C., Ammour, N. Bournet, Khiatti, M., Cao, S., Marzaini, Dewi Syafriyetti Soeis, Rahayujati, Baning, Gunawan, L., Mubarika Haryana, S., Hartono, Michael, Intansari, Umi, Paramita, Dewi Kartikawati, Akbar, Akmal, Hermawan, Benny, Paramita, Dewi K., Argy, Gabriella, Sihotang, Theodora Caroline, Wahyono, Daniel Joko, Soeharso, Purnomo, Suryandari, Dwi Anita, Lisnawati, Musa, Zanil, Daker, Maelinda, Tzen, Yeo Jiun, Bakar, Norhasimah, Rahman, Asma’ Saiyidatina Aishah Abdul, Ahmad, Munirah, Chia, Yeo Tiong, Beng, Alan Khoo Soo, Sasikirana, Widyandani, Wardana, Tirta, Radifar, Muhammad, Herawati, Cita, Surono, Agus, and Çocuk Sağlığı ve Hastalıkları

Subjects
Meeting Abstracts
Abstract

A1 Hope and despair in the current treatment of nasopharyngeal cancer, IB Tan, I1 NPC international incidence and risk factors, Ellen T Chang, I2 Familial nasopharyngeal carcinoma and the use of biomarkers, Chien-Jen Chen, Wan-Lun Hsu, Yin-Chu Chien, I3 Genetic susceptibility risk factors for sporadic and familial NPC: recent findings, Allan Hildesheim, I5 Genetic and environmental risk factors for nasopharyngeal cancer in Southeast Asia, James D McKay, Valerie Gaborieau, Mohamed Arifin Bin Kaderi, Dewajani Purnomosari, Catherine Voegele, Florence LeCalvez-Kelm, Graham Byrnes, Paul Brennan, Beena Devi, I6 Characterization of the NPC methylome identifies aberrant epigenetic disruption of key signaling pathways and EBV-induced gene methylation, Li L, Zhang Y, Fan Y, Sun K, Du Z, Sun H, Chan AT, Tsao SW, Zeng YX, Tao Q, I7 Tumor exosomes and translational research in NPC, Pierre Busson, Claire Lhuillier, Olivier Morales, Dhafer Mrizak, Aurore Gelin, Nikiforos Kapetanakis, Nadira Delhem, I8 Host manipulations of the Epstein-Barr virus EBNA1 protein, Sheila Mansouri, Jennifer Cao, Anup Vaidya, and Lori Frappier, I9 Somatic genetic changes in EBV-associated nasopharyngeal carcinoma, Lo Kwok Wai, I10 Preliminary screening results for nasopharyngeal carcinoma with ELISA-based EBV antibodies in Southern China, Sui-Hong Chen, Jin-lin Du, Ming-Fang Ji, Qi-Hong Huang, Qing Liu, Su-Mei Cao, I11 EBV array platform to screen for EBV antibodies associated with NPC and other EBV-associated disorders, Denise L. Doolan, Anna Coghill, Jason Mulvenna, Carla Proietti, Lea Lekieffre, Jeffrey Bethony, and Allan Hildesheim, I12 The nasopharyngeal carcinoma awareness program in Indonesia, Renske Fles, Sagung Rai Indrasari, Camelia Herdini, Santi Martini, Atoillah Isfandiari, Achmad Rhomdoni, Marlinda Adham, Ika Mayangsari, Erik van Werkhoven, Maarten Wildeman, Bambang Hariwiyanto, Bambang Hermani, Widodo Ario Kentjono, Sofia Mubarika Haryana, Marjanka Schmidt, IB Tan, I13 Current advances and future direction in nasopharyngeal cancer management, Brian O’Sullivan, I14 Management of juvenile nasopharyngeal cancer, Enis Ozyar, I15 Global pattern of nasopharyngeal cancer: correlation of outcome with access to radiotherapy, Anne WM Lee, I16 The predictive/prognostic biomarker for nasopharyngeal carcinoma, Mu-Sheng Zeng, I17 Effect of HLA and KIR polymorphism on NPC risk, Xiaojiang Gao, Minzhong Tang, Pat Martin, Yi Zeng, Mary Carrington, I18 Exploring the Association between Potentially Neutralizing Antibodies against EBV Infection and Nasopharyngeal Carcinoma, Anna E Coghill, Wei Bu, Hanh Nguyen, Wan-Lun Hsu, Kelly J Yu, Pei-Jen Lou, Cheng-Ping Wang, Chien-Jen Chen, Allan Hildesheim, Jeffrey I Cohen, I19 Advances in MR imaging in NPC, Ann D King, O1 Epstein-Barr virus seromarkers and risk of nasopharyngeal carcinoma: the gene-environment interaction study on nasopharyngeal carcinoma in Taiwan, Yin-Chu Chien, Wan-Lun Hsu, Kelly J Yu, Tseng-Cheng Chen, Ching-Yuan Lin, Yung-An Tsou, Yi-Shing Leu, Li-Jen Laio, Yen-Liang Chang, Cheng-Ping Wang, Chun-Hun Hua, Ming-Shiang Wu, Chu-Hsing Kate Hsiao, Jehn-Chuan Lee, Ming-Hsui Tsai, Skye Hung-Chun Cheng, Pei-Jen Lou, Allan Hildesheim, Chien-Jen Chen, O2 Familial tendency and environmental co-factors of nasopharyngeal carcinoma: the gene-environment interaction study on nasopharyngeal carcinoma in Taiwan, Wan-Lun Hsu, Kelly J Yu, Yin-Chu Chien, Tseng-Cheng Chen, Ching-Yuan Lin, Yung-An Tsou, Yi-Shing Leu, Li-Jen Liao, Yen-Liang Chang, Tsung-Lin Yang, Chun-Hun Hua, Ming-ShiangWu, Chu-Hsing Kate Hsiao, Jehn-ChuanLee, Ming-Hsui Tsai, Skye Hung-Chun Cheng, Jenq-Yuh Ko, Allan Hildesheim, Chien-Jen Chen, O3 The genetic susceptibility and prognostic role of TERT-CLPTM1L and genes in DNA damage pathways in NPC, Josephine Mun Yee Ko, Wei Dai, Dora Kwong, Wai Tong Ng, Anne Lee, Roger Kai Cheong Ngan, Chun Chung Yau, Stewart Tung, Maria Li Lung, O4 Long term effects of NPC screening, Mingfang Ji, Wei Sheng, Mun Hon Ng, Weimin Cheng, Xia Yu, Biaohua Wu, Kuangrong Wei, Jun Zhan, Yi Xin Zeng, Su Mei Cao, Ningshao Xia, Yong Yuan, O5 Risk prediction of nasopharyngeal carcinoma by detecting host genetic and Epstein-Barr virus variation in saliva, Qian Cui, Miao Xu, Jin-Xin Bei, Yi-Xin Zeng, O6 Patterns of care study in Turkish nasopharyngeal cancer patients (NAZOTURK): A Turkish Radiation Oncology Association Head and Neck Cancer Working Group Study, B Şahin, A Dizman, M Esassolak, A Saran İkizler, HC Yıldırım, M Çaloğlu, B Atalar, F Akman, C Demiroz, BM Atasoy, E Canyilmaz, S Igdem, G Ugurluer, T Kütük, M Akmansoy, E Ozyar, O7 Long term outcome of intensity modulated radiotherapy in nasopharyngeal carcinoma in National Cancer Centre Singapore, Kiattisa Sommat, Fu Qiang Wang, Li-Lian Kwok, Terence Tan, Kam Weng Fong, Yoke Lim Soong, Shie Lee Cheah, Joseph Wee, O8 International phase II randomized study on the addition of docetaxel to the combination of cisplatin and 5-fluorouracil in the induction treatment for nasopharyngeal carcinoma in children and adolescents, M Casanova, E Özyar, C Patte, D Orbach, A Ferrari, VF Cristine, H Errihani, J Pan, L Zhang, S Liji, K Grzegorzewski, L Gore, A Varan, O9 Prognostic impact of metastatic status in patients with nasopharyngeal carcinoma, Susanna Hilda Hutajulu, Guntara Khuzairi, Camelia Herdini, Henry Kusumo, Mardiah Suci Hardianti, Kartika Widayati Taroeno-Hariadi, Ibnu Purwanto, Johan Kurnianda, O10 Development of small molecule inhibitors of latent Epstein-Barr virus infection for the treatment of nasopharyngeal carcinoma, Troy E. Messick, Kimberly Malecka, Lois Tolvinski, Samantha Soldan, Julianna Deakyne, Hui Song, Antonio van den Heuvel, Baiwei Gu, Joel Cassel, Mark McDonnell, Garry R Smith, Venkata Velvadapu, Haiyan Bian, Yan Zhang, Marianne Carlsen, Shuai Chen, Alastair Donald, Christian Lemmen, Allen B Reitz, Paul M Lieberman, O11 Therapeutic targeting of cancer stem-like cells using a Wnt modulator, ICG-001, enhances the treatment outcome of EBV-positive nasopharyngeal carcinoma, King Chi Chan, Lai Sheung Chan, Kwok Wai Lo, Timothy Tak Chun Yip, Roger Kai Cheong Ngan, Michael Kahn, Maria Li Lung, Nai Ki Mak, O12 Role of micro-RNA in NPC biology, Fei-Fei Liu, O13 Expansion of EBNA1- and LMP2-specific effector T lymphocytes from patients with nasopharyngeal carcinoma without enhancement of regulatory T cells, Wafa Khaali; Juliette Thariat; Laurence Fantin; Flavia Spirito; Meriem Khyatti; El Khalil Ben Driss; Sylvain Olivero; Janet Maryanski; Alain Doglio, O14 The experience of patients’ life after amifostine radiotherapy treatment (ART) for nasopharyngeal carcinoma (NPC), Mengxue Xia, Yunfei Xia, Hui Chang, Rachel Shaw, O15 Analysis of mitochondrial DNA mutation in latent membrane protein-1 positive nasopharyngeal carcinoma, Pudji Rahaju, O16 Factors influencing treatment adherence of nasopharyngeal cancer and the clinical outcomes: a hospital-based study, Mardiah Suci Hardianti, Sindhu Wisesa, Kartika Widayati Taroeno-Harijadi, Ibnu Purwanto, Bambang Hariwiyanto, Wigati Dhamiyati, Johan Kurnianda, O17 Chromosomal breaks mediated by bile acid-induced apoptosis in nasopharyngeal epithelial cells: in relation to matrix association region/scaffold attachment region, Sang-Nee Tan, Sai-Peng Sim, O18 Expression of p53 (wild type) on nasopharyngeal carcinoma stem cell that resistant to radiotherapy, Muhtarum Yusuf, Ahmad C Romdhoni, Widodo Ario K, Fedik Abdul Rantam, O19 Mathematical model of nasopharyngeal carcinoma in cellular level, Sugiyanto, Lina Aryati, Fajar Adi-Kusumo, Mardiah Suci Hardianti, O20 Differential expression of microRNA-21 on nasopharyngeal carcinoma plasma patient, SY Bintoro, R Oktriani, C. Herawati, A Surono, Sofia M. Haryana, O21 Therapeutic targeting of an oncogenic fibroblast growth factor-FGF19, which promotes proliferation and induces EMT of carcinoma cells through activating ERK and AKT signaling, L. Zhong, L. Li, B. B. Ma, A. T. Chan, Q. Tao, O22 Resist nasopharyngeal carcinoma (NPC): next generation T cells for the adoptive immunotherapy of NPC, M. Kalra, M. Ngo, S. Perna, A. Leen, N. Lapteva, C. M. Rooney, S. Gottschalk, O23 The correlation of heat shock protein 70 expressions and staging of nasopharyngeal carcinoma, Elida Mustikaningtyas, Sri Herawati, Achmad C Romdhoni, O24 Epstein-Barr virus serological profiles of nasopharyngeal carcinoma - A tribute to Werner Henle, Mingfang Ji, YaruiXu, Weimin Cheng, ShengxiangGe, Fugui Li, M. H. Ng, O25 Targeting the apoptosis pathway using combination TLR3 agonist with anti-survivin molecule (YM-155) in nasopharyngeal carcinoma, Louise SY Tan, Benjamin Wong, CM Lim, O26 The resistance mechanism of nasopharyngeal cancer stem cells to cisplatin through expression of CD44, Hsp70, p53 (wild type), Oct-4, and ß-catenin encoded-genes, Achmad C Romdhoni, Fedik A. Rantam, Widodo Ario Kentjono, P1 Prevalence of nasopharyngeal carcinoma patients at Departement of Otorhinolaringology-Head and Neck Surgery, Dr. Hasan Sadikin general hospital, Bandung, Indonesia in 2010-2014, Deasy Z Madani, Nur Akbar, Agung Dinasti Permana, P2 Case report on pediatric nasopharyngeal carcinoma at Dr. Sardjito Hospital, Yogyakarta, Camelia Herdini, Sagung Rai Indrasari, Jajah Fachiroh, Dwi Hartati, T. Baning Rahayudjati, P3 Report on loco regionally advanced nasopharyngeal cancer patients treated with induction chemotherapy followed by concurrent chemo-radiation therapy, Iswandi Darwis, Susanna Hilda Hutajulu, Bambang Hariwiyanto, Wigati Dhamiyati, Ibnu Purwanto, Kartika Widayati Taroeno-Hariadi, Johan Kurnianda, P4 Sex and age differences in the survival of patients with nasopharyngeal carcinoma, Sindhu Wisesa, Mardiah Suci Hardianti, Susanna Hilda Hutajulu, Kartika Widayati Taroeno-Harijadi, Ibnu Purwanto, Camelia Herdini, Wigati Dhamiyati, Johan Kurnianda, P5 Impact of delayed diagnosis and delayed therapy in the treatment outcome of patients with nasopharyngeal carcinoma, Khoirul Anwar, Susanna Hilda Hutajulu, Sagung Rai Indrasari, Sri Retna Dwidanarti, Ibnu Purwanto, Kartika Widayati Taroeno-Hariadi, Johan Kurnianda, P6 Anaysis of pretreatment anemia in nasopharyngeal cancer patients undergoing neoadjuvant therapy, Dominicus Wendhy Pramana, Susanna Hilda Hutajulu, Bambang Hariwiyanto, Wigati Dhamiyati, Ibnu Purwanto, Kartika Widayati Taroeno-Hariadi, Johan Kurnianda, P7 Results of treatment with neoadjuvant cisplatin-5FU in locally advanced nasopharyngeal carcinoma: a local experience, Diah Ari Safitri, Susanna Hilda Hutajulu, Camelia Herdini, Sri Retna Dwi Danarti, Ibnu Purwanto, Kartika Widayati Taroeno-Hariadi, Johan Kurnianda, P8 Geriatrics with nasopharyngeal cancer, Suryo A Taroeno, Sindhu Wisesa, Kartika Widayati Taroeno-Hariadi, Ibnu Purwanto, Bambang Hariwiyanto, Wigati Dhamiyati, Johan Kurnianda, P9 Correlation of lymphocyte to monocyte and neutrophil to lymphocyte ratio to the response of cisplatin chemoradiotheraphy in locally advance nasopharyngeal carcinoma, I. Wijaya, A. Oehadian, D. Prasetya, P10 Prediction of nasopharyngeal carcinoma risk by Epstein-Barr virus seromarkers and environmental co-factors: the gene-environment interaction study on nasopharyngeal carcinoma in Taiwan, Wan-Lun Hsu, Yin-Chu Chien, Kelly J Yu, Cheng-Ping Wang, Ching-Yuan Lin, Yung-An Tsou, Yi-Shing Leu, Li-Jen Liao, Yen-Liang Chang191,192, Jenq-Yuh Ko, Chun-Hun Hua, Ming-Shiang Wu, Chu-Hsing Kate Hsiao, Jehn-Chuan Lee, Ming-Hsui Tsai, Skye Hung-Chun Cheng, Pei-Jen Lou, Allan Hildesheim, Chien-Jen Chen, P11 Non-viral risk factors for nasopharyngeal carcinoma in West Sumatra, Indonesia, Sukri Rahman, Bestari J. Budiman, Novialdi, Rahmadona, Dewi Yuri Lestari, P12 New prototype Vidas EBV IgA quick: performance on Chinese and Moroccan populations, C. Yin, A. Foussadier, E. Blein, C. Chen, N. Bournet Ammour, M. Khiatti, S. Cao, P13 The expression of EBV-LMP1 and VEGF as predictors and plasma EBV-DNA levels as early marker of distant metastasis after therapy in nasopharyngeal cancer, Dewi Syafriyetti Soeis Marzaini, P14 Characteristics and factors influencing subjects refusal for blood samples retrieval: lesson from NPC case control study in Yogyakarta – Indonesia, Dwi Hartati, Baning Rahayujati, Camelia Herdini, Jajah Fachiroh, P15 Expression of microRNA BART-7-3p and mRNA PTEN on blood plasma of patients with nasopharyngeal carcinoma, L. Gunawan, S. Mubarika Haryana, A. Surono, C. Herawati, P16 IgA response to native early antigen (IgA-EAext) of Epstein-Barr virus (EBV) in healthy population and nasopharyngeal carcinoma (NPC) patients: the potential for diagnosis and screening tools, Michael Hartono, Jajah Fachiroh, Umi Intansari, Dewi Kartikawati Paramita, P17 IgA responses against Epstein-Barr Virus Early Antigen (EBV-EA) peptides as potential candidates of nasopharyngeal carcinoma detection marker, Akmal Akbar, Jajah Fachiroh, Dewi Kartikawati Paramita, P18 Association between smoking habit and IgA-EBV titer among healthy individuals in Yogyakarta, Indonesia, Benny Hermawan, T Baning Rahayudjati, Dewi K Paramita, Jajah Fachiroh, P19 Epstein-Barr virus IgA titer comparison of healthy non-family individuals and healthy first degree family of NPV patients, Gabriella Argy, Jajah Fachiroh, Dewi Kartikawati Paramita, Susanna Hilda Hutajulu, P20 Identification of EBV Early Antigen (EA) derived peptides for NPC diagnosis, Theodora Caroline Sihotang, Jajah Fachiroh, Umi Intansari, Dewi Kartikawati Paramita, P21 Host-pathogen study: relative expression of mRNA BRLF1 Epstein-Barr virus as a potential biomarker for tumor progressivity and polymorphisms of TCRBC and TCRGC2 host genes related to genetic susceptibility on nasopharyngeal carcinoma, Daniel Joko Wahyono, Purnomo Soeharso, Dwi Anita Suryandari, Lisnawati, Zanil Musa, Bambang Hermani, P22 In vitro efficacy of silvestrol and episilvestrol, isolated from Borneo, on nasopharyngeal carcinoma, a major cancer in Borneo, Maelinda Daker, Yeo Jiun Tzen, Norhasimah Bakar, Asma’ Saiyidatina Aishah Abdul Rahman, Munirah Ahmad, Yeo Tiong Chia, Alan Khoo Soo Beng, P23 The expression of mir-141 in patients with nasopharyngeal cancer, Widyandani Sasikirana, Tirta Wardana, Muhammad Radifar, Cita Herawati, Agus Surono, Sofia Mubarika Haryana

Published
2016

24. Identification of new susceptibility loci for IgA nephropathy in Han Chinese.

Author

Li, M, Foo, J-N, Wang, J-Q, Low, H-Q, Tang, X-Q, Toh, K-Y, Yin, P-R, Khor, C-C, Goh, Y-F, Irwan, ID, Xu, R-C, Andiappan, AK, Bei, J-X, Rotzschke, O, Chen, M-H, Cheng, C-Y, Sun, L-D, Jiang, G-R, Wong, T-Y, Lin, H-L, Aung, T, Liao, Y-H, Saw, S-M, Ye, K, Ebstein, RP, Chen, Q-K, Shi, W, Chew, S-H, Chen, J, Zhang, F-R, Li, S-P, Xu, G, Shyong Tai, E, Wang, L, Chen, N, Zhang, X-J, Zeng, Y-X, Zhang, H, Liu, Z-H, Yu, X-Q, Liu, J-J, Li, M, Foo, J-N, Wang, J-Q, Low, H-Q, Tang, X-Q, Toh, K-Y, Yin, P-R, Khor, C-C, Goh, Y-F, Irwan, ID, Xu, R-C, Andiappan, AK, Bei, J-X, Rotzschke, O, Chen, M-H, Cheng, C-Y, Sun, L-D, Jiang, G-R, Wong, T-Y, Lin, H-L, Aung, T, Liao, Y-H, Saw, S-M, Ye, K, Ebstein, RP, Chen, Q-K, Shi, W, Chew, S-H, Chen, J, Zhang, F-R, Li, S-P, Xu, G, Shyong Tai, E, Wang, L, Chen, N, Zhang, X-J, Zeng, Y-X, Zhang, H, Liu, Z-H, Yu, X-Q, and Liu, J-J

Abstract

IgA nephropathy (IgAN) is one of the most common primary glomerulonephritis. Previously identified genome-wide association study (GWAS) loci explain only a fraction of disease risk. To identify novel susceptibility loci in Han Chinese, we conduct a four-stage GWAS comprising 8,313 cases and 19,680 controls. Here, we show novel associations at ST6GAL1 on 3q27.3 (rs7634389, odds ratio (OR)=1.13, P=7.27 × 10(-10)), ACCS on 11p11.2 (rs2074038, OR=1.14, P=3.93 × 10(-9)) and ODF1-KLF10 on 8q22.3 (rs2033562, OR=1.13, P=1.41 × 10(-9)), validate a recently reported association at ITGAX-ITGAM on 16p11.2 (rs7190997, OR=1.22, P=2.26 × 10(-19)), and identify three independent signals within the DEFA locus (rs2738058, P=1.15 × 10(-19); rs12716641, P=9.53 × 10(-9); rs9314614, P=4.25 × 10(-9), multivariate association). The risk variants on 3q27.3 and 11p11.2 show strong association with mRNA expression levels in blood cells while allele frequencies of the risk variants within ST6GAL1, ACCS and DEFA correlate with geographical variation in IgAN prevalence. Our findings expand our understanding on IgAN genetic susceptibility and provide novel biological insights into molecular mechanisms underlying IgAN.

Published
2015

25. A common variant near TGFBR3 is associated with primary open angle glaucoma

Author

Li, Z, Allingham, RR, Nakano, M, Jia, L, Chen, Y, Ikeda, Y, Mani, B, Chen, L-J, Kee, C, Garway-Heath, DF, Sripriya, S, Fuse, N, Abu-Amero, KK, Huang, C, Namburi, P, Burdon, K, Perera, SA, Gharahkhani, P, Lin, Y, Ueno, M, Ozaki, M, Mizoguchi, T, Krishnadas, SR, Osman, EA, Lee, MC, Chan, ASY, Tajudin, L-SA, Do, T, Goncalves, A, Reynier, P, Zhang, H, Bourne, R, Goh, D, Broadway, D, Husain, R, Negi, AK, Su, DH, Ho, C-L, Blanco, AA, Leung, CKS, Wong, TT, Yakub, A, Liu, Y, Nongpiur, ME, Han, JC, Hon, DN, Shantha, B, Zhao, B, Sang, J, Zhang, N, Sato, R, Yoshii, K, Panda-Jonas, S, Koch, AEA, Herndon, LW, Moroi, SE, Challa, P, Foo, JN, Bei, J-X, Zeng, Y-X, Simmons, CP, Tran, NBC, Sharmila, PF, Chew, M, Lim, B, Tam, POS, Chua, E, Ng, XY, Yong, VHK, Chong, YF, Meah, WY, Vijayan, S, Seongsoo, S, Xu, W, Teo, YY, Bailey, JNC, Kang, JH, Haines, JL, Cheng, CY, Saw, S-M, Tai, E-S, Richards, JE, Ritch, R, Gaasterland, DE, Pasquale, LR, Liu, J, Jonas, JB, Milea, D, George, R, Al-Obeidan, SA, Mori, K, Macgregor, S, Hewitt, AW, Girkin, CA, Zhang, M, Sundaresan, P, Vijaya, L, Mackey, DA, Wong, TY, Craig, JE, Sun, X, Kinoshita, S, Wiggs, JL, Khor, C-C, Yang, Z, Pang, CP, Wang, N, Hauser, MA, Tashiro, K, Aung, T, Vithana, EN, Li, Z, Allingham, RR, Nakano, M, Jia, L, Chen, Y, Ikeda, Y, Mani, B, Chen, L-J, Kee, C, Garway-Heath, DF, Sripriya, S, Fuse, N, Abu-Amero, KK, Huang, C, Namburi, P, Burdon, K, Perera, SA, Gharahkhani, P, Lin, Y, Ueno, M, Ozaki, M, Mizoguchi, T, Krishnadas, SR, Osman, EA, Lee, MC, Chan, ASY, Tajudin, L-SA, Do, T, Goncalves, A, Reynier, P, Zhang, H, Bourne, R, Goh, D, Broadway, D, Husain, R, Negi, AK, Su, DH, Ho, C-L, Blanco, AA, Leung, CKS, Wong, TT, Yakub, A, Liu, Y, Nongpiur, ME, Han, JC, Hon, DN, Shantha, B, Zhao, B, Sang, J, Zhang, N, Sato, R, Yoshii, K, Panda-Jonas, S, Koch, AEA, Herndon, LW, Moroi, SE, Challa, P, Foo, JN, Bei, J-X, Zeng, Y-X, Simmons, CP, Tran, NBC, Sharmila, PF, Chew, M, Lim, B, Tam, POS, Chua, E, Ng, XY, Yong, VHK, Chong, YF, Meah, WY, Vijayan, S, Seongsoo, S, Xu, W, Teo, YY, Bailey, JNC, Kang, JH, Haines, JL, Cheng, CY, Saw, S-M, Tai, E-S, Richards, JE, Ritch, R, Gaasterland, DE, Pasquale, LR, Liu, J, Jonas, JB, Milea, D, George, R, Al-Obeidan, SA, Mori, K, Macgregor, S, Hewitt, AW, Girkin, CA, Zhang, M, Sundaresan, P, Vijaya, L, Mackey, DA, Wong, TY, Craig, JE, Sun, X, Kinoshita, S, Wiggs, JL, Khor, C-C, Yang, Z, Pang, CP, Wang, N, Hauser, MA, Tashiro, K, Aung, T, and Vithana, EN

Abstract

Primary open angle glaucoma (POAG), a major cause of blindness worldwide, is a complex disease with a significant genetic contribution. We performed Exome Array (Illumina) analysis on 3504 POAG cases and 9746 controls with replication of the most significant findings in 9173 POAG cases and 26 780 controls across 18 collections of Asian, African and European descent. Apart from confirming strong evidence of association at CDKN2B-AS1 (rs2157719 [G], odds ratio [OR] = 0.71, P = 2.81 × 10(-33)), we observed one SNP showing significant association to POAG (CDC7-TGFBR3 rs1192415, ORG-allele = 1.13, Pmeta = 1.60 × 10(-8)). This particular SNP has previously been shown to be strongly associated with optic disc area and vertical cup-to-disc ratio, which are regarded as glaucoma-related quantitative traits. Our study now extends this by directly implicating it in POAG disease pathogenesis.

Published
2015

27. ABCC5, a Gene That Influences the Anterior Chamber Depth, Is Associated with Primary Angle Closure Glaucoma

Author

Wiggs, JL, Nongpiur, ME, Khor, CC, Jia, H, Cornes, BK, Chen, L-J, Qiao, C, Nair, KS, Cheng, C-Y, Xu, L, George, R, Do, T, Abu-Amero, K, Perera, SA, Ozaki, M, Mizoguchi, T, Kurimoto, Y, Low, S, Tajudin, L-SA, Ho, C-L, Tham, CCY, Soto, I, Chew, PTK, Wong, H-T, Shantha, B, Kuroda, M, Osman, EA, Tang, G, Fan, S, Meng, H, Wang, H, Feng, B, Yong, VHK, Ting, SML, Li, Y, Wang, Y-X, Li, Z, Lavanya, R, Wu, R-Y, Zheng, Y-F, Su, DH, Loon, S-C, Allingham, RR, Hauser, MA, Soumittra, N, Ramprasad, VL, Waseem, N, Yaakub, A, Chia, K-S, Kumaramanickavel, G, Wong, TT, How, AC, Tran, NBC, Simmons, CP, Bei, J-X, Zeng, Y-X, Bhattacharya, SS, Zhang, M, Tan, DT, Teo, Y-Y, Al-Obeidan, SA, Do, NH, Tai, E-S, Saw, S-M, Foster, PJ, Vijaya, L, Jonas, JB, Wong, T-Y, John, SWM, Pang, C-P, Vithana, EN, Wang, N, Aung, T, Wiggs, JL, Nongpiur, ME, Khor, CC, Jia, H, Cornes, BK, Chen, L-J, Qiao, C, Nair, KS, Cheng, C-Y, Xu, L, George, R, Do, T, Abu-Amero, K, Perera, SA, Ozaki, M, Mizoguchi, T, Kurimoto, Y, Low, S, Tajudin, L-SA, Ho, C-L, Tham, CCY, Soto, I, Chew, PTK, Wong, H-T, Shantha, B, Kuroda, M, Osman, EA, Tang, G, Fan, S, Meng, H, Wang, H, Feng, B, Yong, VHK, Ting, SML, Li, Y, Wang, Y-X, Li, Z, Lavanya, R, Wu, R-Y, Zheng, Y-F, Su, DH, Loon, S-C, Allingham, RR, Hauser, MA, Soumittra, N, Ramprasad, VL, Waseem, N, Yaakub, A, Chia, K-S, Kumaramanickavel, G, Wong, TT, How, AC, Tran, NBC, Simmons, CP, Bei, J-X, Zeng, Y-X, Bhattacharya, SS, Zhang, M, Tan, DT, Teo, Y-Y, Al-Obeidan, SA, Do, NH, Tai, E-S, Saw, S-M, Foster, PJ, Vijaya, L, Jonas, JB, Wong, T-Y, John, SWM, Pang, C-P, Vithana, EN, Wang, N, and Aung, T

Abstract

Anterior chamber depth (ACD) is a key anatomical risk factor for primary angle closure glaucoma (PACG). We conducted a genome-wide association study (GWAS) on ACD to discover novel genes for PACG on a total of 5,308 population-based individuals of Asian descent. Genome-wide significant association was observed at a sequence variant within ABCC5 (rs1401999; per-allele effect size = -0.045 mm, P = 8.17 × 10(-9)). This locus was associated with an increase in risk of PACG in a separate case-control study of 4,276 PACG cases and 18,801 controls (per-allele OR = 1.13 [95% CI: 1.06-1.22], P = 0.00046). The association was strengthened when a sub-group of controls with open angles were included in the analysis (per-allele OR = 1.30, P = 7.45 × 10(-9); 3,458 cases vs. 3,831 controls). Our findings suggest that the increase in PACG risk could in part be mediated by genetic sequence variants influencing anterior chamber dimensions.

Published
2014

28. Genome-wide association analyses identify three new susceptibility loci for primary angle closure glaucoma

Author

Vithana, EN, Khor, C-C, Qiao, C, Nongpiur, ME, George, R, Chen, L-J, Tan, D, Abu-Amero, KK, Huang, CK, Low, S, Tajudin, L-SA, Perera, SA, Cheng, C-Y, Xu, L, Jia, H, Ho, C-L, Sim, KS, Wu, R-Y, Tham, CCY, Chew, PTK, Su, DH, Oen, FT, Sarangapani, S, Soumittra, N, Osman, EA, Wong, H-T, Tang, G, Fan, S, Meng, H, Huong, DTL, Wang, H, Feng, B, Baskaran, M, Shantha, B, Ramprasad, VL, Kumaramanickavel, G, Iyengar, SK, How, AC, Lee, KY, Sivakumaran, TA, Yong, VHK, Ting, SML, Li, Y, Wang, Y-X, Tay, W-T, Sim, X, Lavanya, R, Cornes, BK, Zheng, Y-F, Wong, TT, Loon, S-C, Yong, VKY, Waseem, N, Yaakub, A, Chia, K-S, Allingham, RR, Hauser, MA, Lam, DSC, Hibberd, ML, Bhattacharya, SS, Zhang, M, Teo, YY, Tan, DT, Jonas, JB, Tai, E-S, Saw, S-M, Do, NH, Al-Obeidan, SA, Liu, J, Tran, NBC, Simmons, CP, Bei, J-X, Zeng, Y-X, Foster, PJ, Vijaya, L, Wong, T-Y, Pang, C-P, Wang, N, Aung, T, Vithana, EN, Khor, C-C, Qiao, C, Nongpiur, ME, George, R, Chen, L-J, Tan, D, Abu-Amero, KK, Huang, CK, Low, S, Tajudin, L-SA, Perera, SA, Cheng, C-Y, Xu, L, Jia, H, Ho, C-L, Sim, KS, Wu, R-Y, Tham, CCY, Chew, PTK, Su, DH, Oen, FT, Sarangapani, S, Soumittra, N, Osman, EA, Wong, H-T, Tang, G, Fan, S, Meng, H, Huong, DTL, Wang, H, Feng, B, Baskaran, M, Shantha, B, Ramprasad, VL, Kumaramanickavel, G, Iyengar, SK, How, AC, Lee, KY, Sivakumaran, TA, Yong, VHK, Ting, SML, Li, Y, Wang, Y-X, Tay, W-T, Sim, X, Lavanya, R, Cornes, BK, Zheng, Y-F, Wong, TT, Loon, S-C, Yong, VKY, Waseem, N, Yaakub, A, Chia, K-S, Allingham, RR, Hauser, MA, Lam, DSC, Hibberd, ML, Bhattacharya, SS, Zhang, M, Teo, YY, Tan, DT, Jonas, JB, Tai, E-S, Saw, S-M, Do, NH, Al-Obeidan, SA, Liu, J, Tran, NBC, Simmons, CP, Bei, J-X, Zeng, Y-X, Foster, PJ, Vijaya, L, Wong, T-Y, Pang, C-P, Wang, N, and Aung, T

Abstract

Primary angle closure glaucoma (PACG) is a major cause of blindness worldwide. We conducted a genome-wide association study including 1,854 PACG cases and 9,608 controls across 5 sample collections in Asia. Replication experiments were conducted in 1,917 PACG cases and 8,943 controls collected from a further 6 sample collections. We report significant associations at three new loci: rs11024102 in PLEKHA7 (per-allele odds ratio (OR)=1.22; P=5.33×10(-12)), rs3753841 in COL11A1 (per-allele OR=1.20; P=9.22×10(-10)) and rs1015213 located between PCMTD1 and ST18 on chromosome 8q (per-allele OR=1.50; P=3.29×10(-9)). Our findings, accumulated across these independent worldwide collections, suggest possible mechanisms explaining the pathogenesis of PACG.

Published
2012

29. Epigenetic disruption of interferon-gamma response through silencing the tumor suppressor interferon regulatory factor 8 in nasopharyngeal, esophageal and multiple other carcinomas

Author

Lee, K.Y., Geng, H., Ng, K.M., Yu, J., van Hasselt, A., Cao, Y., Zeng,Y-X, Wong, A.H.Y., Wang, X., Ying, J., Srivastava, G., Lung, M.L., Wang,L-D, Kwok, T.T., Levi,B-Z, Chan, A.T.C., Sung, J.J.Y., Tao, Q., Lee, K.Y., Geng, H., Ng, K.M., Yu, J., van Hasselt, A., Cao, Y., Zeng,Y-X, Wong, A.H.Y., Wang, X., Ying, J., Srivastava, G., Lung, M.L., Wang,L-D, Kwok, T.T., Levi,B-Z, Chan, A.T.C., Sung, J.J.Y., and Tao, Q.

Abstract

16q24 is frequently deleted in multiple tumors including cancers of nasopharynx, esophagus, breast, prostate and liver. By array comparative genomic hybridization (aCGH), we refined a 16q24 hemizygous deletion in nasopharyngeal carcinoma (NPC) cell lines. Semi-quantitative RT-PCR analysis revealed interferon regulatory factor 8 (IRF8) as the only downregulated gene within this deletion. IRF8 belongs to a family of interferon (IFN) regulatory factors that modulate various important physiologic processes including host defense, cell growth and differentiation and immune regulation. In contrast to the broad expression of IRF8 in normal adult and fetal tissues, transcriptional silencing and promoter methylation of IRF8 were frequently detected in multiple carcinoma (except for hepatocellular) cell lines (100\% in NPC, 88\% in esophageal and 18-78\% in other carcinoma cell lines) and in a large collection of primary carcinomas (78\% in NPC, 36-71\% in other carcinomas). Methylation of the IRF8 promoter led to the disruption of its response to IFN-gamma stimulation. Pharmacological and genetic demethylation could restore IRF8 expression, indicating a direct epigenetic mechanism. Ectopic expression of IRF8 in tumor cells lacking its expression strongly inhibited their clonogenicity, confirming its tumor suppressor function. Thus, IRF8 was identified as a functional tumor suppressor, which is frequently silenced by epigenetic mechanism in multiple carcinomas.

Published
2008

30. MAGNETIC FIELD DISTRIBUTION IN THE ELECTROMAGNETIC FEEDING RISER OF RECTANGLE STEEL INGOT.

Author

XU, C. J., ZENG, Y. X., WANG, Z. L., LI, J., LI, S. L., and ZHANG, X. J.

Subjects
*STEEL ingots, *ELECTROMAGNETISM, *MAGNETIC fields, *COMPUTER simulation, *ELECTROMAGNETIC induction
Abstract

The electromagnetic feeding method was used to improve solidification quality of steel ingot, and the feeding principle of electromagnetic riser was introduced. The distribution characteristics of magnetic field in electromagnetic riser were investigated before and after pouring steel by the measures of numerical and physical simulation. The results showed that there's a good symmetry of magnetic field distribution in electromagnetic feeding riser before pouring steel, but both symmetry and uniformity of magnetic field distribution are broken after pouring steel into riser, and magnetic induction intensity of the surface is higher than that of the inner of feeding riser. [ABSTRACT FROM AUTHOR]

Published
2016

31. Two Epstein-Barr Virus-Related Serologic Antibody Tests in Nasopharyngeal Carcinoma Screening: Results From the Initial Phase of a Cluster Randomized Controlled Trial in Southern China

Author

Liu, Z., primary, Ji, M.-F., additional, Huang, Q.-H., additional, Fang, F., additional, Liu, Q., additional, Jia, W.-H., additional, Guo, X., additional, Xie, S.-H., additional, Chen, F., additional, Liu, Y., additional, Mo, H.-Y., additional, Liu, W.-L., additional, Yu, Y.-L., additional, Cheng, W.-M., additional, Yang, Y.-Y., additional, Wu, B.-H., additional, Wei, K.-R., additional, Ling, W., additional, Lin, X., additional, Lin, E.-H., additional, Ye, W., additional, Hong, M.-H., additional, Zeng, Y.-X., additional, and Cao, S.-M., additional

Published
2012
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33. Acetylcholinesterase expression mediated by c-Jun-NH2-terminal kinase pathway during anticancer drug-induced apoptosis

Author

Deng, R., Li, W., Guan, Z., Zhou,J-M, Wang, Y., Mei,Y-P, Li,M-T, Feng,G-K, Huang, W., Liu,Z-C, Han, Y., Zeng,Y-X, Zhu,X-F, Deng, R., Li, W., Guan, Z., Zhou,J-M, Wang, Y., Mei,Y-P, Li,M-T, Feng,G-K, Huang, W., Liu,Z-C, Han, Y., Zeng,Y-X, and Zhu,X-F

Abstract

It has been shown that acetylcholinesterase (AChE) expression was induced during apoptosis and the antisense oligonucleotides and siRNA of AChE may prevent apoptosis in various cell types. However, the mechanisms underlying AChE upregulation remain elusive. We demonstrated here that c-Jun NH2-terminal kinase (JNK) could mediate AChE expression. In this study, both etoposide and excisanin A, two anticancer agents, induced apoptosis in colon cancer cell line SW620 as determined by Annexin V staining, the cleavage of caspase-3 and the proteolytic degradation of poly (ADPribose) polymerase (PARP). The results showed that both the agents upregulated AChE in SW620 cells. In the meantime, JNK was also activated and the expression and phosphorylation of c-Jun increased in SW620 cells exposed to the two agents. The induced AChE mRNA and protein expression could be blocked by SP600125, a specific inhibitor of SAPK/JNK, and small interfering RNA directed against JNK1/2. Transfection with adenovirus-mediated dominant negative c-Jun also blocked the upregulation of AChE expression. Together, these results suggest that AChE expression may be mediated by the activation of JNK pathway during apoptosis through a c-Jun-dependent mechanism.

Published
2006

35. EZH2 supports nasopharyngeal carcinoma cell aggressiveness by forming a co-repressor complex with HDAC1/HDAC2 and Snail to inhibit E-cadherin

Author

Tong, Z-T, primary, Cai, M-Y, additional, Wang, X-G, additional, Kong, L-L, additional, Mai, S-J, additional, Liu, Y-H, additional, Zhang, H-B, additional, Liao, Y-J, additional, Zheng, F, additional, Zhu, W, additional, Liu, T-H, additional, Bian, X-W, additional, Guan, X-Y, additional, Lin, M C, additional, Zeng, M-S, additional, Zeng, Y-X, additional, Kung, H-F, additional, and Xie, D, additional

Published
2011
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36. Anthracyclines disrupt telomere maintenance by telomerase through inducing PinX1 ubiquitination and degradation

Author

Zhang, B, primary, Qian, D, additional, Ma, H-H, additional, Jin, R, additional, Yang, P-X, additional, Cai, M-Y, additional, Liu, Y-H, additional, Liao, Y-J, additional, Deng, H-X, additional, Mai, S-J, additional, Zhang, H, additional, Zeng, Y-X, additional, Lin, M C, additional, Kung, H-F, additional, Xie, D, additional, and Huang, J-J, additional

Published
2011
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43. Expression of cytoplasmic and nuclear Survivin in primary and secondary human glioblastoma.

Author

Xie, D., Zeng, Y. X., Wang, H. J., Wen, J. M., Tao, Y., Sham, J. S. T., and Guan, X. Y.

Subjects
*GLIOMAS, *NERVOUS system tumors, *APOPTOSIS, *TUMORS, *CARCINOGENESIS, *PHENOTYPES
Abstract

Clinically, human glioblastoma (GBM) may develop de novo or from a low-grade glioma (secondary GBM), and molecular alterations in the two pathways may differ. This study examined the status of Survivin expression and apoptosis in 30 primary and 26 secondary GBMs. Our results show that cytoplasmic Survivin positivity was significantly (P<0.001) more frequent in primary GBMs (83%) than that in secondary GBMs (46%). In addition, an inverse correlation of cytoplasmc Survivin positivity with GBM apoptotic index, and a positive association between cytoplasmic Survivin and size of the tumours were observed. These results suggest that cytoplasmic Survivin, via its antiapoptotic function, may be involved in the tumorigenesis of many primary GBMs, but only in a small fraction of secondary GBMs. Furthermore, the overall progression times from low-grade precursor lesions to secondary GBMs were significantly shorter (P<0.05) in cytoplasmic Survivin-positive cases (mean, 15.6 months) than those in Survivin-negative cases (mean, 23.8 moths), and the positive expression level of Survivin in cytoplasm was upregulated in most secondary GBMs when compared to matched pre-existing low-graded lesions. These results suggest that the increased accumulation of Survivin in the cytoplasm of more malignant glioma cells may prove to be a selective advantage, thus accelerating progression to a more aggressive phenotype. [ABSTRACT FROM AUTHOR]

Published
2006
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45. [Expression of anaplastic lymphoma kinase fusion gene in patients with lung sarcomatoid carcinoma and treatment analysis].

Author

Niu HT, Dong P, Wang JN, Zeng YX, Yuan W, and Yuan P

Subjects
Anaplastic Lymphoma Kinase, Humans, Neoplasm Recurrence, Local, Protein Kinase Inhibitors, Pyrazoles, Carcinoma, Non-Small-Cell Lung, Lung Neoplasms
Abstract

Objective: To investigate the expression status of anaplastic lymphoma kinase (ALK) fusion gene in lung sarcomatoid carcinoma (LSC) and the role of ALK inhibitors for treatment. Methods: Total of 84 cases of LSC confirmed by histopathology were detected for ALK fusion gene from January 2011 to December 2014 in the Cancer Hospital of Chinese Academy of Medical Science&Peking Union Medical College and Shandong Zibo Wanjie Cancer Hospital. All patients were primarily treated by the multi-disciplinary mode in combination with chemotherapy or targeted therapy based on surgery. Postoperative adjuvant chemotherapy was given on platinum based two-drug combination regimen. In ALK fusion gene (+ ) patients with recurrence or metastasis, crizotinib target therapy was prefered. Chi-square test was applied for the comparison of 1, 3, 5-year survival rates between the two groups. Results: Eighty-two cases completed the follow-up. ALK fusion gene was found in 9(10.7%) patients. After application of crizotinib, 1 case was evaluated as complete remission, 6 cases as partial response, 2 cases as stable disease; the 1, 3, 5-year survival rate was 100% (9/9), 100% (9/9) and 88.9% (8/9) for the patients with ALK fusion gene, and it was 65.8% (48/73), 15.1% (11/73) and 6.8% (5/73) respectively for patients without ALK fusion gene. There was significant difference in the survival rate between the two groups (χ(2)=1.56, 1.56, 0.83, all P <0.05). Conclusion: ALK fusion gene maybe expressed in LSC patients. Compared with conventional chemotherapy, crizotinib can significantly prolong the survival time of patients with ALK fusion gene.

Published
2018
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46. [Analysis of treatment and prognosis in post-operative patients with urachal carcinoma].

Author

Niu HT, Dong P, Wang JN, Huang J, and Zeng YX

Subjects
Adenocarcinoma mortality, Humans, Lymph Node Excision, Male, Middle Aged, Neoplasm Recurrence, Local, Neoplasm Staging, Prognosis, Prospective Studies, Retrospective Studies, Survival Rate, Treatment Outcome, Urinary Bladder Neoplasms mortality, Adenocarcinoma pathology, Adenocarcinoma surgery, Cystectomy, Postoperative Period, Urinary Bladder Neoplasms pathology, Urinary Bladder Neoplasms surgery
Abstract

Objective: Urachal carcinoma is a kind of urogenital tract malignancy with a very low incidence. The objective of this study was to observe the clinical presentation, pathological condition, treatment method and outcome of patients with urachal carcinoma., Methods: A retrospective analysis of thirty-six cases of urachal carcinoma diagnosed over a period of 10 years from 2003 to 2013 was carried out. All pathologic specimens were reviewed by two separate pathologists. Clinical and histological features, treatment condition, patient follow-up and survival outcome was reviewed and calculated., Results: The mean age at diagnosis was 53 years. Of the thirty-six patients, twenty-five were male. All patients underwent partial cystectomy with bilateral pelvic lymph node dissection. All cases were adenocarcinoma, including 20 mucinous adenocarcinoma, 7 moderately differentiated adenocarcinoma, 5 poorly differentiated adenocarcinoma, 1 signet ring cell carcinoma, 3 hybrid adenocarcinoma. The Sheldon pathologic stage was stage Ⅱ in 11, Ⅲ in 16 and Ⅳa in 9 cases. All patients received medical oncological therapy. The median follow-up period was 27 months. The median overall survival was 36 months. One-year survival rate was 70% and five-year survival rate was 28%., Conclusions: Urachal carcinomas are rare and usually at locally advanced stage at diagnosis with a high tendency of metastases. Surgery is a key method of primary treatment and medical oncological therapy may play a role in decreasing the chances of recurrence which still needs to be explained by prospective clinical trials.

Published
2016
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47. Mapping quantitative trait loci for sheath blight disease resistance in Yangdao 4 rice.

Author

Wen ZH, Zeng YX, Ji ZJ, and Yang CD

Subjects
Alleles, China, Chromosomes, Plant genetics, Genetic Linkage, Genetic Markers, Oryza microbiology, Phenotype, Phylogeography, Plant Diseases microbiology, Rhizoctonia isolation & purification, Chromosome Mapping, Disease Resistance genetics, Oryza genetics, Quantitative Trait Loci
Abstract

Rice sheath blight (ShB), which is caused by Rhizoctonia solani, has become the most serious rice disease in China. Yangdao 4, a cultivar with partial resistance to ShB, was crossed with Lemont, a susceptible cultivar, to develop mapping populations that were used to analyze quantitative trait loci (QTL) that confer resistance to ShB. QTL analysis were performed in 3 environments (E1-E3) using 2 F2 and 1 F2:3 populations, respectively. Three traits were recorded to evaluate ShB resistance, including disease rating (DR), lesion height (LH), and percentage of lesion height (PLH). Based on field evaluation of ShB resistance and the 2 genetic maps constructed, we identified a total of 8 QTLs for DR (4 in E1, 4 in E2, and 3 in E3), 6 QTLs for LH (1 in E1, 3 in E2, and 2 in E3), and 7 QTLs for PLH (1 in E1, 4 in E2, and 2 in E3). Sixteen of the ShB-QTLs co-localized as 6 clusters on chromosomes 3, 7, 11, and 12. Four of the 6 clusters contained ShB-QTLs that were detected in 2 environments, while the other 2 clusters with ShB-QTLs were detected in 1 environment. Three ShB-QTLs (qSBD-3-2, qSBL-3-1, and qSBPL-3-1) were delimited to a 581-kb region flanked by markers D333B and D334 on chromosome 3. The resistance alleles of Yangdao 4 at the qSBD-3-2 locus decreased DR by 0.68 and 0.79 in E2 and E3, respectively.

Published
2015
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48. Development of 1047 insertion-deletion markers for rice genetic studies and breeding.

Author

Zeng YX, Wen ZH, Ma LY, Ji ZJ, Li XM, and Yang CD

Subjects
Chromosomes, Plant, Genetic Linkage, Genome, Plant, Physical Chromosome Mapping, Polymorphism, Genetic, Breeding, Genetic Markers, INDEL Mutation, Oryza genetics
Abstract

In this study, a total of 1047 insertion-deletion (InDel) primer pairs distributed across the rice genome were developed and experimentally validated. The primer pairs were designed based on the InDel length polymorphisms between 93-11 (Oryza sativa ssp indica cv.) and Nipponbare (Oryza sativa ssp japonica cv.), aiming for utilization between indica and japonica rice, or between other inter-subspecific rice cultivars. The 1047 primer pairs were dispersed across all 12 of the rice chromosomes, with one InDel marker found every 371.3 kb on average. The InDel length of the markers varied from 3 to 39 bp: 88.2% of the markers contained 6 to 25 bp, only 6.2% of markers were ≤ 5 bp, and 5.6% were ≥ 26 bp. Six hundred and twenty-three (59.5%) of the 1047 InDel markers were shown to amplify well and were polymorphic between Taichung65 and IR8, and 476 (45.5%) markers were polymorphic between Lemont and Yangdao4, while 398 (38.0%) were polymorphic in both combinations. These results demonstrated that the polymerase chain reaction-based InDel markers developed in this study could be of immediate use for rice genetic studies and breeding programs.

Published
2013
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49. Multiple drug resistance phenotype of human endothelial cells induced by vascular endothelial growth factor 165.

Author

Zhang XS, Zhu XF, Gao JS, Qian CN, Kuang ZJ, Liu ZC, and Zeng YX

Subjects
Angiogenesis Inhibitors pharmacology, Camptothecin pharmacology, Capillaries cytology, Capillaries metabolism, Endothelium, Vascular metabolism, Epirubicin pharmacology, Humans, Irinotecan, Paclitaxel pharmacology, Phenotype, Skin blood supply, Antineoplastic Agents pharmacology, Camptothecin analogs & derivatives, Drug Resistance, Multiple genetics, Drug Resistance, Neoplasm genetics, Endothelium, Vascular cytology, Vascular Endothelial Growth Factor A pharmacology
Abstract

Aim: To investigate the effect of vascular endothelial growth factor 165 (VEGF165) on sensitivity of endothelial cells to anticancer drugs., Methods: Human dermal microvessel endothelial cells (HDMEC) were incubated with anticancer drugs in the presence of VEGF165. Survival of endothelial cells was assayed by MTT method. DNA fragments of apoptosis were detected by agarose electrophoresis. Potential mechanisms underlying the effect of VEGF165 on endothelial cells were investigated with RT-PCR and Western blot analysis., Results: VEGF165 induced the multidrug resistance phenotype of HDMEC to a wide variety of anticancer drugs such as epirubicin, cisplatin, etoposide, mytomycin C, vincristine, CPT-11, and taxol in vitro. This protective effect was partly due to the up-regulation of lung drug resistance protein (LRP) and multidrug resistance-associated protein (MRP), as well as the down-regulation of Bax protein induced by VEGF165., Conclusion: VEGF165 induced multidrug resistance phenotype of endothelial cells, which implicated the anti-angiogenic effect of anticancer drugs might depend on microenvironment of tumors in vivo.

Published
2001

50. Apoptosis induced by ceramide in hepatocellular carcinoma Bel7402 cells.

Author

Zhu XF, Zhang XS, Li ZM, Yao YQ, Xie BF, Liu ZC, and Zeng YX

Subjects
Carcinoma, Hepatocellular metabolism, Down-Regulation, Humans, Liver Neoplasms metabolism, Proto-Oncogene Proteins metabolism, Proto-Oncogene Proteins c-bcl-2 metabolism, Tumor Cells, Cultured, Tumor Suppressor Protein p53 metabolism, bcl-2-Associated X Protein, Apoptosis drug effects, Carcinoma, Hepatocellular pathology, Ceramides pharmacology, Liver Neoplasms pathology
Abstract

Aim: To study the biological function of ceramide signaling in Bel7402 cells., Methods: Inhibition of cell growth was assayed using MTT method. Morphologic assessment of apoptosis was performed with fluorescence microscope. DNA fragmentation was detected by electrophoresis and flow cytometry. The levels of protein p53, Bcl-2, and Bax were measured with Western blot., Results: Bel7402 cells treated with C2-ceramide underwent cell proliferation inhibition. IC50 value was 14.28 mumol.L-1. After treatment of Bel7402 with ceramide, the morphologic changes including reduction in volume, nuclear chromatin condensation, fluorescence strength were observed. SubG1 peaks were detected on flow cytometry (FCM). Agarose gel electrophoresis of DNA from cells treated with ceramide revealed "ladder" pattern. The Western blot assay from cell extracts showed that the levels of protein p53 were decreased after ceramide treatment. The levels of protein Bcl-2 were decreased also. But the levels of Bax protein showed no difference between untreated cells and treated cells., Conclusion: Ceramide induces apoptosis in Bel7402 cells, related to Bcl-2 down-regulation.

Published
2000

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