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862 results on '"Zhang, Baohong"'

8. A protein kinase C α and β inhibitor blunts hyperphagia to halt renal function decline and reduces adiposity in a rat model of obesity-driven type 2 diabetes

Author

Wang, Ju, Casimiro-Garcia, Agustin, Johnson, Bryce G., Duffen, Jennifer, Cain, Michael, Savary, Leigh, Wang, Stephen, Nambiar, Prashant, Lech, Matthew, Zhao, Shanrong, Xi, Li, Zhan, Yutian, Olson, Jennifer, Stejskal, James A., Lin, Hank, Zhang, Baohong, Martinez, Robert V., Masek-Hammerman, Katherine, Schlerman, Franklin J., and Dower, Ken

Published
2023
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16. CHOmics: A web-based tool for multi-omics data analysis and interactive visualization in CHO cell lines.

Author

Lin, Dongdong, Yalamanchili, Hima B, Zhang, Xinmin, Lewis, Nathan E, Alves, Christina S, Groot, Joost, Arnsdorf, Johnny, Bjørn, Sara P, Wulff, Tune, Voldborg, Bjørn G, Zhou, Yizhou, and Zhang, Baohong

Subjects
Bioinformatics, Mathematical Sciences, Biological Sciences, Information and Computing Sciences
Abstract

Chinese hamster ovary (CHO) cell lines are widely used in industry for biological drug production. During cell culture development, considerable effort is invested to understand the factors that greatly impact cell growth, specific productivity and product qualities of the biotherapeutics. While high-throughput omics approaches have been increasingly utilized to reveal cellular mechanisms associated with cell line phenotypes and guide process optimization, comprehensive omics data analysis and management have been a challenge. Here we developed CHOmics, a web-based tool for integrative analysis of CHO cell line omics data that provides an interactive visualization of omics analysis outputs and efficient data management. CHOmics has a built-in comprehensive pipeline for RNA sequencing data processing and multi-layer statistical modules to explore relevant genes or pathways. Moreover, advanced functionalities were provided to enable users to customize their analysis and visualize the output systematically and interactively. The tool was also designed with the flexibility to accommodate other types of omics data and thereby enabling multi-omics comparison and visualization at both gene and pathway levels. Collectively, CHOmics is an integrative platform for data analysis, visualization and management with expectations to promote the broader use of omics in CHO cell research.

Published
2020

23. Cx3cr1-deficient microglia exhibit a premature aging transcriptome.

Author

Gyoneva, Stefka, Hosur, Raghavendra, Gosselin, David, Zhang, Baohong, Ouyang, Zhengyu, Cotleur, Anne C, Peterson, Michael, Allaire, Norm, Challa, Ravi, Cullen, Patrick, Roberts, Chris, Miao, Kelly, Reynolds, Taylor L, Glass, Christopher K, Burkly, Linda, and Ransohoff, Richard M

Subjects
Microglia, Neurons, Animals, Mice, Inbred C57BL, Mice, Transgenic, Mice, Knockout, Mice, Disease Models, Animal, Inflammation, Aging, Premature, Lipopolysaccharides, Receptors, Chemokine, Signal Transduction, Gene Deletion, Female, Male, Transcriptome, CX3C Chemokine Receptor 1, Genetic Profile, Inbred C57BL, Transgenic, Knockout, Disease Models, Animal, Aging, Premature, Receptors, Chemokine
Abstract

CX3CR1, one of the highest expressed genes in microglia in mice and humans, is implicated in numerous microglial functions. However, the molecular mechanisms underlying Cx3cr1 signaling are not well understood. Here, we analyzed transcriptomes of Cx3cr1-deficient microglia under varying conditions by RNA-sequencing (RNA-seq). In 2-mo-old mice, Cx3cr1 deletion resulted in the down-regulation of a subset of immune-related genes, without substantial epigenetic changes in markers of active chromatin. Surprisingly, Cx3cr1-deficient microglia from young mice exhibited a transcriptome consistent with that of aged Cx3cr1-sufficient animals, suggesting a premature aging transcriptomic signature. Immunohistochemical analysis of microglia in young and aged mice revealed that loss of Cx3cr1 modulates microglial morphology in a comparable fashion. Our results suggest that CX3CR1 may regulate microglial function in part by modulating the expression levels of a subset of inflammatory genes during chronological aging, making Cx3cr1-deficient mice useful for studying aged microglia.

Published
2019

34. Broad ultra-potent neutralization of SARS-CoV-2 variants by monoclonal antibodies specific to the tip of RBD

Author

Ma, Hang, Guo, Yingying, Tang, Haoneng, Tseng, Chien-Te K., Wang, Lei, Zong, Huifang, Wang, Zhenyu, He, Yang, Chang, Yunsong, Wang, Shusheng, Huang, Haiqiu, Ke, Yong, Yuan, Yunsheng, Wu, Mingyuan, Zhang, Yuanyuan, Drelich, Aleksandra, Kempaiah, Kempaiah Rayavara, Peng, Bi-Hung, Wang, Ailin, Yang, Kaiyong, Yin, Haiyang, Liu, Junjun, Yue, Yali, Xu, Wenbo, Zhu, Shuangli, Ji, Tianjiao, Zhang, Xiaoju, Wang, Ziqi, Li, Gang, Liu, Guangchun, Song, Jingjing, Mu, Lingling, Xiang, ZongShang, Song, Zhangyi, Chen, Hua, Bian, Yanlin, Zhang, Baohong, Chen, Hui, Zhang, Jiawei, Liao, Yunji, Zhang, Li, Yang, Li, Chen, Yi, Gilly, John, Xiao, Xiaodong, Han, Lei, Jiang, Hua, Xie, Yueqing, Zhou, Qiang, and Zhu, Jianwei

Published
2022
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39. High‐quality chromosome‐level genome assembly and multi‐omics analysis of rosemary (Salvia rosmarinus) reveals new insights into the environmental and genome adaptation

Author

Lai, Yong, primary, Ma, Jinghua, additional, Zhang, Xuebin, additional, Xuan, Xiaobo, additional, Zhu, Fengyun, additional, Ding, Shen, additional, Shang, Fude, additional, Chen, Yuanyuan, additional, Zhao, Bing, additional, Lan, Chen, additional, Unver, Turgay, additional, Huo, George, additional, Li, Ximei, additional, Wang, Yihan, additional, Liu, Yufang, additional, Lu, Mengfei, additional, Pan, Xiaoping, additional, Yang, Deshuang, additional, Li, Mingwan, additional, Zhang, Baohong, additional, and Zhang, Dangquan, additional

Published
2024
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41. Identification, characterization, and gene expression analysis of nucleotide binding site (NB)-type resistance gene homologues in switchgrass

Author

Frazier, Taylor P, Palmer, Nathan A, Xie, Fuliang, Tobias, Christian M, Donze-Reiner, Teresa J, Bombarely, Aureliano, Childs, Kevin L, Shu, Shengqiang, Jenkins, Jerry W, Schmutz, Jeremy, Zhang, Baohong, Sarath, Gautam, and Zhao, Bingyu

Subjects
Biological Sciences, Bioinformatics and Computational Biology, Genetics, Alleles, Amino Acid Sequence, Computational Biology, Databases, Nucleic Acid, Disease Resistance, Gene Expression Profiling, Genes, Plant, Genetic Association Studies, Genetic Predisposition to Disease, Genome, Plant, Genomics, Panicum, Phylogeny, Polymorphism, Single Nucleotide, Position-Specific Scoring Matrices, Protein Interaction Domains and Motifs, Reproducibility of Results, Biofuel, Disease resistance, Gene expression, NB-LRR, Panicum virgatum, RNA-seq, SNP, Information and Computing Sciences, Medical and Health Sciences, Bioinformatics, Biological sciences, Biomedical and clinical sciences
Abstract

BackgroundSwitchgrass (Panicum virgatum L.) is a warm-season perennial grass that can be used as a second generation bioenergy crop. However, foliar fungal pathogens, like switchgrass rust, have the potential to significantly reduce switchgrass biomass yield. Despite its importance as a prominent bioenergy crop, a genome-wide comprehensive analysis of NB-LRR disease resistance genes has yet to be performed in switchgrass.ResultsIn this study, we used a homology-based computational approach to identify 1011 potential NB-LRR resistance gene homologs (RGHs) in the switchgrass genome (v 1.1). In addition, we identified 40 RGHs that potentially contain unique domains including major sperm protein domain, jacalin-like binding domain, calmodulin-like binding, and thioredoxin. RNA-sequencing analysis of leaf tissue from 'Alamo', a rust-resistant switchgrass cultivar, and 'Dacotah', a rust-susceptible switchgrass cultivar, identified 2634 high quality variants in the RGHs between the two cultivars. RNA-sequencing data from field-grown cultivar 'Summer' plants indicated that the expression of some of these RGHs was developmentally regulated.ConclusionsOur results provide useful insight into the molecular structure, distribution, and expression patterns of members of the NB-LRR gene family in switchgrass. These results also provide a foundation for future work aimed at elucidating the molecular mechanisms underlying disease resistance in this important bioenergy crop.

Published
2016

50. Re-Engineering Therapeutic Anti-Aβ Monoclonal Antibody to Target Amyloid Light Chain.

Author

Bai, Jingyi, Li, Xi, Zhao, Jun, Zong, Huifang, Yuan, Yuan, Wang, Lei, Zhang, Xiaoshuai, Ke, Yong, Han, Lei, Xu, Jianrong, Ma, Buyong, Zhang, Baohong, and Zhu, Jianwei

Subjects
MONOCLONAL antibodies, PEPTIDES, AMYLOID, CD20 antigen, ALZHEIMER'S disease, AMYLOID beta-protein, CYTOTOXINS
Abstract

Amyloidosis involves the deposition of misfolded proteins. Even though it is caused by different pathogenic mechanisms, in aggregate, it shares similar features. Here, we tested and confirmed a hypothesis that an amyloid antibody can be engineered by a few mutations to target a different species. Amyloid light chain (AL) and β-amyloid peptide (Aβ) are two therapeutic targets that are implicated in amyloid light chain amyloidosis and Alzheimer's disease, respectively. Though crenezumab, an anti-Aβ antibody, is currently unsuccessful, we chose it as a model to computationally design and prepare crenezumab variants, aiming to discover a novel antibody with high affinity to AL fibrils and to establish a technology platform for repurposing amyloid monoclonal antibodies. We successfully re-engineered crenezumab to bind both Aβ42 oligomers and AL fibrils with high binding affinities. It is capable of reversing Aβ42-oligomers-induced cytotoxicity, decreasing the formation of AL fibrils, and alleviating AL-fibrils-induced cytotoxicity in vitro. Our research demonstrated that an amyloid antibody could be engineered by a few mutations to bind new amyloid sequences, providing an efficient way to reposition a therapeutic antibody to target different amyloid diseases. [ABSTRACT FROM AUTHOR]

Published
2024
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