19 results on '"Zhihe Qing"'
Search Results
2. Platinum Nanoparticles Loaded Graphitic Carbon Nitride Nanosheets with Enhanced Peroxidase-like Activity for H2O2 and Oxidase-Based Sensing
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Gege Yang, Ying Chen, Rui Shi, Rongrong Chen, Shanshan Gao, Xin Zhang, Yuan Rao, Ying Lu, Yuancheng Peng, Zhihe Qing, and Chunxia Song
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PtNP@g–C3N4 nanosheets ,peroxidase-mimic ,H2O2 ,oxidase-based sensing ,Organic chemistry ,QD241-441 - Abstract
Platinum nanoparticles (PtNPs) are classical peroxidase-like nanozyme; self-agglomeration of nanoparticles leads to the undesirable reduction in stability and catalytic activity. Herein, a hybrid peroxidase-like nanocatalyst consisting of PtNPs in situ growing on g–C3N4 nanosheets with enhanced peroxidase-mimic catalytic activity (PtNP@g–C3N4 nanosheets) was prepared for H2O2 and oxidase-based colorimetric assay. g–C3N4 nanosheets can be used as carriers to solve the problem of poor stability of PtNPs. We observed that the catalytic ability could be maintained for more than 90 days. PtNP@g–C3N4 nanosheets could quickly catalyze the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB), and the absorbance of blue color oxidized TMB (oxTMB) showed a robust linear relationship with the concentration of H2O2 (the detection limit (LOD): 3.33 μM). By utilizing H2O2 as a mediator, this strategy can be applied to oxidase-based biomolecules (glucose, organophosphorus, and so on, that generate or consume hydrogen peroxide) sensing. As a proof of concept, a sensitive assay of cholesterol that combined PtNP@g–C3N4 nanosheets with cholesterol oxidase (ChOx) cascade catalytic reaction was constructed with an LOD of 9.35 μM in a widespread range from 10 to 800 μM (R2 = 0.9981). In addition, we also verified its ability to detect cholesterol in fetal bovine serum. These results showed application prospect of PtNP@g–C3N4 nanosheets-based colorimetry in sensing and clinical medical detection.
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- 2023
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3. Editorial: Advances in Nucleic Acid-Based Biosensors and Imaging
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Jane Ru Choi, Mithran Somasundrum, Muhammad J.A. Shiddiky, Werasak Surareungchai, Yufang Hu, and Zhihe Qing
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nucleic acid ,DNA/RNA ,sensing ,diagnostics ,imaging ,detection ,Chemistry ,QD1-999 - Published
- 2022
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4. MIL/Aptamer as a Nanosensor Capable of Resisting Nonspecific Displacement for ATP Imaging in Living Cells
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Jun Li, Yuedong Zhang, Zhen Zou, Zhihe Qing, Sheng Yang, Jianxiao Yang, Lihua Zhang, Feng Feng, and Ronghua Yang
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Chemistry ,QD1-999 - Published
- 2019
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5. Natural Peptide Probe Screened for High-Performance Fluorescent Sensing of Copper Ion: Especially Sensitivity, Rapidity, and Environment-Friendliness
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Xiaoxuan Li, Zhihe Qing, Younan Li, Zhen Zou, Sheng Yang, and Ronghua Yang
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Chemistry ,QD1-999 - Published
- 2019
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6. DNA-Templated Fluorescent Nanoclusters for Metal Ions Detection
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Chunxia Song, Jingyuan Xu, Ying Chen, Liangliang Zhang, Ying Lu, and Zhihe Qing
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dna-template ,fluorescence ,nanoclusters ,metal ions ,detection ,Organic chemistry ,QD241-441 - Abstract
DNA-templated fluorescent nanoclusters (NCs) have attracted increasing research interest on account of their prominent features, such as DNA sequence-dependent fluorescence, easy functionalization, wide availability, water solubility, and excellent biocompatibility. Coupling DNA templates with complementary DNA, aptamers, G-quadruplex, and so on has generated a large number of sensors. Additionally, the preparation and applications of DNA-templated fluorescent NCs in these sensing have been widely studied. This review firstly focuses on the properties of DNA-templated fluorescent NCs, and the synthesis of DNA-templated fluorescent NCs with different metals is then discussed. In the third part, we mainly introduce the applications of DNA-templated fluorescent NCs for sensing metal ions. At last, we further discuss the future perspectives of DNA-templated fluorescent NCs in the synthesis and sensing metal ions in the environmental and biological fields.
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- 2019
- Full Text
- View/download PDF
7. An Activatable Nanoenzyme Reactor for Coenhanced Chemodynamic and Starving Therapy Against Tumor Hypoxia and Antioxidant Defense System
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Juewen Liu, Zhihe Qing, JunBin Li, Yanli Lei, Lifang Chen, Ailing Bai, Shuohui Xing, Ronghua Yang, and Zhen Zou
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Antioxidant ,Tumor hypoxia ,business.industry ,medicine.medical_treatment ,medicine ,Cancer research ,Cancer therapy ,General Chemistry ,business ,Therapeutic strategy - Abstract
It is critical to improve the efficiency of cancer therapy with minimized side effects. Chemodynamic therapy (CDT) is a tumor therapeutic strategy designed to generate abundant reactive oxygen spec...
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- 2021
8. Frontispiz: A Glucose‐Powered Activatable Nanozyme Breaking pH and H 2 O 2 Limitations for Treating Diabetic Infections
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Lifang Chen, Shuohui Xing, Yanli Lei, Qiaoshu Chen, Zhen Zou, Ke Quan, Zhihe Qing, Juewen Liu, and Ronghua Yang
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General Medicine - Published
- 2021
9. An intramolecular catalytic hairpin assembly on a DNA tetrahedron for mRNA imaging in living cells: improving reaction kinetics and signal stability
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Taiping Qing, Zhihe Qing, Jinlei Hu, Yanli Lei, Jingyuan Xu, Zhen Zou, and Ronghua Yang
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Chemical kinetics ,Reaction rate ,chemistry.chemical_compound ,Chemistry ,Förster resonance energy transfer ,Intramolecular force ,Kinetics ,Biophysics ,A-DNA ,General Chemistry ,Transfection ,DNA - Abstract
Enzyme-free amplification techniques based on dynamic DNA self-assembly (DDSA) have recently been developed for the in situ detection of mRNA in living cells. However, signal generation in traditional DDSA amplifiers is mainly dependent on the random diffusion of dissociative probes in a bulk solution, which is generally accompanied by poor kinetics and interference from complex biological systems. In this work, a new amplifier based on the design of an intramolecular catalytic hairpin assembly (intra-CHA) is proposed for the FRET imaging of mRNA in living cells. Compared with that in the free catalytic hairpin assembly (free-CHA), probes H1 and H2 in intra-CHA were simultaneously fixed on a DNA tetrahedron. The distance between them was closer, the local concentration of H1 and H2 in intra-CHA was theoretically approximately 808-times higher than that in free-CHA, and the initial reaction rate was enhanced 15.6 fold. Due to the spatial confinement effect, the reaction kinetics for target-catalyzed signal generation were significantly improved. By virtue of the three-dimensional nanostructure, H1 and H2 in the intra-CHA amplifier entered cells without any transfection or nanocarrier, and the probes and their products were free from biological interference, providing much higher signal stability for the reliable imaging of mRNA in living cells., An intramolecular catalytic hairpin assembly is implemented on a DNA tetrahedron for mRNA imaging in living cells. The spatial confinement effect enables the acceleration of target-triggered signal generation, with excellent cell permeability and FRET signal stability.
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- 2021
10. MIL/Aptamer as a Nanosensor Capable of Resisting Nonspecific Displacement for ATP Imaging in Living Cells
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Yuedong Zhang, Zhihe Qing, Jianxiao Yang, Feng Feng, Lihua Zhang, Zhen Zou, Ronghua Yang, Sheng Yang, and Jun Li
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Quenching (fluorescence) ,Chemistry ,General Chemical Engineering ,Aptamer ,High selectivity ,Nanotechnology ,General Chemistry ,Fluorescence ,Article ,Nanomaterials ,lcsh:Chemistry ,lcsh:QD1-999 ,Nanosensor ,Drug delivery ,Intracellular - Abstract
Fluorescent probes physisorbed on nanomaterials have emerged as a kind of useful and facile sensing platform for biological important molecules. However, nonspecific displacement in the physisorption systems is a non-negligible problem for the intracellular analysis. MIL (Materials of Institut Lavoisier), a subclass of metal–organic frameworks (MOFs), has high porosity, large surface area, and intriguing three-dimensional (3D) nanostructure with promising biological and biomedical applications such as molecular detection and drug delivery. Herein, we report MIL/aptamer-FAM as a nanosensor capable of resisting nonspecific displacement for intracellular adenosinetriphosphate (ATP) sensing and imaging. In this approach, by virtue of the remarkable quenching capability, high affinity of aptamers, and dramatic capability of resisting nonspecific displacement of 3D MIL-100, the assay and imaging of ATP in living cells were realized. Our results demonstrated that the MIL/aptamer-FAM nanosensor not only shows high selectivity for the detection of ATP in buffer but also is able to act as a “signal-on” nanosensor for specific imaging of ATP in living cells. The strategy reported here opens up a new way to develop MOF-based nanosensors for intracellular delivery and metabolite detection.
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- 2019
11. DNA-Templated Fluorescent Nanoclusters for Metal Ions Detection
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Ying Chen, Liangliang Zhang, Ying Lu, Zhihe Qing, Chunxia Song, and Jingyuan Xu
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DNA Replication ,Materials science ,Biocompatibility ,Aptamer ,Metal ions in aqueous solution ,nanoclusters ,detection ,Pharmaceutical Science ,Nanotechnology ,Biosensing Techniques ,Review ,02 engineering and technology ,010402 general chemistry ,01 natural sciences ,behavioral disciplines and activities ,Analytical Chemistry ,Nanoclusters ,lcsh:QD241-441 ,chemistry.chemical_compound ,lcsh:Organic chemistry ,Drug Discovery ,mental disorders ,Physical and Theoretical Chemistry ,Ions ,Molecular Structure ,Organic Chemistry ,metal ions ,DNA ,dna-template ,021001 nanoscience & nanotechnology ,Fluorescence ,Nanostructures ,0104 chemical sciences ,Template ,chemistry ,Metals ,Chemistry (miscellaneous) ,Molecular Medicine ,Surface modification ,fluorescence ,0210 nano-technology - Abstract
DNA-templated fluorescent nanoclusters (NCs) have attracted increasing research interest on account of their prominent features, such as DNA sequence-dependent fluorescence, easy functionalization, wide availability, water solubility, and excellent biocompatibility. Coupling DNA templates with complementary DNA, aptamers, G-quadruplex, and so on has generated a large number of sensors. Additionally, the preparation and applications of DNA-templated fluorescent NCs in these sensing have been widely studied. This review firstly focuses on the properties of DNA-templated fluorescent NCs, and the synthesis of DNA-templated fluorescent NCs with different metals is then discussed. In the third part, we mainly introduce the applications of DNA-templated fluorescent NCs for sensing metal ions. At last, we further discuss the future perspectives of DNA-templated fluorescent NCs in the synthesis and sensing metal ions in the environmental and biological fields.
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- 2019
12. Progress in biosensor based on DNA-templated copper nanoparticles
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Ailing Bai, Shuohui Xing, Kemin Wang, Zhihe Qing, Zhen Zou, Ronghua Yang, and Xiaoxiao He
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Materials science ,Biomedical Engineering ,Biophysics ,Nanoparticle ,Metal Nanoparticles ,Nanotechnology ,02 engineering and technology ,Biosensing Techniques ,01 natural sciences ,Fluorescence ,Nanomaterials ,Molecular recognition ,Electrochemistry ,Microelectronics ,Surface plasmon resonance ,Inductively coupled plasma mass spectrometry ,Fluorescent Dyes ,business.industry ,010401 analytical chemistry ,General Medicine ,DNA ,Surface-enhanced Raman spectroscopy ,Surface Plasmon Resonance ,021001 nanoscience & nanotechnology ,0104 chemical sciences ,Spectrometry, Fluorescence ,0210 nano-technology ,business ,Biosensor ,Copper ,Biotechnology - Abstract
During the last decades, by virtue of their unique physicochemical properties and potential application in microelectronics, biosensing and biomedicine, metal nanomaterials (MNs) have attracted great research interest and been highly developed. Deoxyribonucleic acid (DNA) is a particularly interesting ligand for templating bottom-up nanopreparation, by virtue of its excellent properties including nanosized geometry structure, programmable and artificial synthesis, DNA-metal ion interaction and powerful molecular recognition. DNA-templated copper nanoparticles (DNA-CuNPs) has been developed in recent years. Because of its advantages including simple and rapid preparation, high efficiency, MegaStokes shifting and low biological toxicity, DNA-CuNPs has been highly exploited for biochemical sensing from 2010, especially as a label-free detection manner, holding advantages in multiple analytical technologies including fluorescence, electrochemistry, surface plasmon resonance, inductively coupled plasma mass spectrometry and surface enhanced Raman spectroscopy. This review comprehensively tracks the preparation of DNA-CuNPs and its application in biosensing, and highlights the potential development and challenges regarding this field, aiming to promote the advance of this fertile research area.
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- 2019
13. In situ formation of fluorescent copper nanoparticles for ultrafast zero-background Cu 2+ detection and its toxicides screening
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Ronghua Yang, Zhong Cao, Zhihe Qing, Sheng Yang, Xiaoxiao He, Kemin Wang, and Lixuan Zhu
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In situ ,Biomedical Engineering ,Biophysics ,Analytical chemistry ,Metal Nanoparticles ,Nanoparticle ,chemistry.chemical_element ,Biosensing Techniques ,02 engineering and technology ,010402 general chemistry ,01 natural sciences ,Fluorescence ,Ion ,Electrochemistry ,Humans ,Molecule ,Ions ,Chemistry ,Water ,General Medicine ,021001 nanoscience & nanotechnology ,Acid mine drainage ,Copper ,Emission intensity ,0104 chemical sciences ,0210 nano-technology ,Water Pollutants, Chemical ,Biotechnology - Abstract
Copper pollution has become more and more serious in modern society as the increasing industrial emission and the acid mine drainage, and exposure to excess copper can result in damage to living organisms. Thus, the development of efficient strategy for copper ion (Cu(2+)) detection is very essential and significant. Here, a high-efficiency fluorescent method is proposed for Cu(2+) monitoring. The detection mechanism is based on the in situ formation of fluorescent copper nanoparticles (CuNPs). When the water sample is polluted by Cu(2+), fluorescence emission of CuNPs can be observed by a one-step manner, and the emission intensity is proportional to Cu(2+) concentration. Attractively, besides its advantages in operation and good detection capability, the generation of fluorescent signal is ultrafast, with a good signal response in 1 min; and there is no interference from background and other ions due to the in situ formation of signal unit. By virtue of its advantages, this strategy has been used to detect Cu(2+) from polluted tap and river water samples, good performances demonstrate that the proposed method can be practically applied for Cu(2+) monitoring in real drinking and environmental water. Simultaneously, great potential for Cu(2+) toxicides screening has been verified by direct analysis of the effects of different model molecules on Cu(2+), which will contribute to Cu(2+)-related sewage treatment and medical therapy.
- Published
- 2016
14. SERS assay of telomerase activity at single-cell level and colon cancer tissues via quadratic signal amplification
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Jinfeng Yang, Jing Zheng, Changhui Liu, Ronghua Yang, Guixiang Tan, Zhihe Qing, Sheng Yang, Muling Shi, and Yongjun Tan
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Telomerase ,Silver ,Cell ,Biomedical Engineering ,Biophysics ,Metal Nanoparticles ,Biology ,Spectrum Analysis, Raman ,010402 general chemistry ,Sensitivity and Specificity ,01 natural sciences ,Silver nanoparticle ,HeLa ,chemistry.chemical_compound ,Electrochemistry ,medicine ,Humans ,Detection limit ,010401 analytical chemistry ,Reproducibility of Results ,Cancer ,Equipment Design ,General Medicine ,Surface Plasmon Resonance ,medicine.disease ,biology.organism_classification ,Molecular biology ,0104 chemical sciences ,Enzyme Activation ,Equipment Failure Analysis ,medicine.anatomical_structure ,chemistry ,Colloidal gold ,Colonic Neoplasms ,DNA ,HeLa Cells ,Biotechnology - Abstract
As an important biomarker and therapeutic target, telomerase has attracted extensive attention concerning its detection and monitoring. Recently, enzyme-assisted amplification approaches have provided useful platforms for the telomerase activity detection, however, further improvement in sensitivity is still hindered by the single-step signal amplification. Herein, we develop a quadratic signal amplification strategy for ultrasensitive surface-enhanced Raman scattering (SERS) detection of telomerase activity. The central idea of our design is using telomerase-induced silver nanoparticles (AgNPs) assembly and silver ions (Ag(+))-mediated cascade amplification. In our approach, each telomerase-aided DNA sequence extension could trigger the formation of a long double-stranded DNA (dsDNA), making numerous AgNPs assembling along with this long strand through specific Ag-S bond, to form a primary amplification element. For secondary amplification, each conjugated AgNP was dissolved into Ag(+), which can effectively induce the 4-aminobenzenethiol (4-ABT) modified gold nanoparticles (AuNPs@4-ABT) to undergo aggregation to form numerous "hot-spots". Through quadratic amplifications, a limit of detection down to single HeLa cell was achieved. More importantly, this method demonstrated good performance when applied to tissues from colon cancer patients, which exhibits great potential in the practical application of telomerase-based cancer diagnosis in early stages. To demonstrate the potential in screening the telomerase inhibitors and telomerase-targeted drugs, the proposed design is successfully employed to measure the inhibition of telomerase activity by 3'-azido-3'-deoxythymidine.
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- 2016
15. Graphene biosensors for bacterial and viral pathogens
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Zhihe Qing, Peng Zhang, Bo Feng, Zixin Jiang, Jin Xu, and Taiping Qing
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Environmental evaluation ,Coronavirus disease 2019 (COVID-19) ,Computer science ,Graphene derivatives ,Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) ,Pneumonia, Viral ,Biomedical Engineering ,Biophysics ,Nanotechnology ,02 engineering and technology ,Biosensing Techniques ,01 natural sciences ,Article ,law.invention ,Betacoronavirus ,COVID-19 Testing ,law ,Electrochemistry ,Molecular diagnostic techniques ,Animals ,Humans ,Pandemics ,Bio-functionalization ,Bacteria ,Graphene ,Clinical Laboratory Techniques ,SARS-CoV-2 ,Biosensing ,010401 analytical chemistry ,technology, industry, and agriculture ,COVID-19 ,General Medicine ,021001 nanoscience & nanotechnology ,0104 chemical sciences ,Molecular Diagnostic Techniques ,Clinical diagnosis ,Viruses ,Graphite ,Pathogens ,0210 nano-technology ,Coronavirus Infections ,Biosensor ,Biotechnology - Abstract
The infection and spread of pathogens (e.g., COVID-19) pose an enormous threat to the safety of human beings and animals all over the world. The rapid and accurate monitoring and determination of pathogens are of great significance to clinical diagnosis, food safety and environmental evaluation. In recent years, with the evolution of nanotechnology, nano-sized graphene and graphene derivatives have been frequently introduced into the construction of biosensors due to their unique physicochemical properties and biocompatibility. The combination of biomolecules with specific recognition capabilities and graphene materials provides a promising strategy to construct more stable and sensitive biosensors for the detection of pathogens. This review tracks the development of graphene biosensors for the detection of bacterial and viral pathogens, mainly including the preparation of graphene biosensors and their working mechanism. The challenges involved in this field have been discussed, and the perspective for further development has been put forward, aiming to promote the development of pathogens sensing and the contribution to epidemic prevention., Highlights • The infection and spread of pathogens threaten human health, the development of effective detection strategies is highly important and essential. • Graphene biosensor as one of effective nano-platforms is reviewed in the field of pathogen detection. • The preparation of graphene biosensors and their sensing mechanism are introduced and discussed. • The current challenges in this field and the perspective for further development are presented, which will be meaningful for pathogens sensing and epidemic prevention.
- Published
- 2020
16. Programmed packaging of mesoporous silica nanocarriers for matrix metalloprotease 2-triggered tumor targeting and release
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Jun Xiong, Kemin Wang, Zhihe Qing, Liling Li, Xue Yang, Zhen Zou, Li Wen, Dinggeng He, Xiaoxiao He, and Linli Cai
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Male ,food.ingredient ,Materials science ,Biophysics ,Antineoplastic Agents ,Bioengineering ,Nanotechnology ,Ligands ,Endocytosis ,Gelatin ,Cell Line ,Biomaterials ,Mice ,Folic Acid ,food ,Microscopy, Electron, Transmission ,In vivo ,Cell Line, Tumor ,Neoplasms ,PEG ratio ,medicine ,Animals ,Humans ,Doxorubicin ,Drug Carriers ,Mice, Inbred BALB C ,Neoplasms, Experimental ,Mesoporous silica ,Silicon Dioxide ,Liver ,Mechanics of Materials ,Culture Media, Conditioned ,Ceramics and Composites ,Matrix Metalloproteinase 2 ,Nanoparticles ,Nanocarriers ,Mesoporous material ,HT29 Cells ,Neoplasm Transplantation ,medicine.drug - Abstract
The development of multifunctional nanocarrier with each unit functioning at the correct time and location is a challenge for clinical applications. With this in mind, a type of intelligent mesoporous silica nanocarrier (PGFMSN) is proposed for matrix metalloprotease 2 (MMP 2)-triggered tumor targeting and release by integrating programmed packing and MMP 2-degradable gelatin. Mesoporous silica nanoparticles (MSN) are first functionalized with folic acid (FA) as a target ligand to improve cell uptake. Then gelatin is introduced onto FA-MSN via temperature-induced gelation, where gelatin layer blocks drugs inside the mesopores and protects the targeting ligand. To prolong blood-circulation lifetime, PEG is further decorated to obtain PGFMSN. All units are programmatically incorporated in a simple way and coordinated in an optimal fashion. Cells, multicellular spheroids and in vivo results demonstrate that PGFMSN is shielded against nonspecific uptake. After circulating to tumor tissue, the up-regulated MMP-2 hydrolyzes gelatin layer to deshield PEG and switch on the function of FA, which facilitate the selective uptake by tumor cells through folate-receptor-mediated endocytosis. Meanwhile, the packaged drug is released due to the shedding of gelatin layer. It is shown that doxorubicin (DOX)-loaded exhibits superior tumor targeting, drug internalization, cytotoxicity, and antitumor efficacy over free DOX, non-PEGylated and non-targeted nanoparticles, which provides potential applications for targeted cancer therapy.
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- 2015
17. dsDNA-templated fluorescent copper nanoparticles: poly(AT-TA)-dependent formation
- Author
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Zhihe Qing, Taiping Qing, Xiaoxiao He, Kemin Wang, Li Wen, Fengzhou Xu, Hui Shi, Zhengui Mao, and Dinggeng He
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Chemical engineering ,Chemistry ,General Chemical Engineering ,Nanoparticle ,chemistry.chemical_element ,Nanotechnology ,Sequence (biology) ,General Chemistry ,Copper ,Fluorescence - Abstract
In this work, poly(AT-TA) is found as the specific sequence composition which contributes to the formation of dsDNA-templated fluorescent copper nanoparticles. The finding will be helpful in wide fields, such as constructing DNA-templated nanodevices and designing biochemical nano-probes.
- Published
- 2014
18. Activatable aptamer probe for contrast-enhanced in vivo cancer imaging based on cell membrane protein-triggered conformation alteration
- Author
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Zhihe Qing, Kemin Wang, Hui Shi, Xiaoxiao He, Bing Zhou, Xiaosheng Ye, Weihong Tan, Xiaohai Yang, Xu Wu, and Qiuping Guo
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Protein Conformation ,Aptamer ,Biology ,Cell Line ,Cell membrane ,In vivo ,Neoplasms ,medicine ,Humans ,Multidisciplinary ,Base Sequence ,Cancer ,Membrane Proteins ,Aptamers, Nucleotide ,Biological Sciences ,medicine.disease ,Flow Cytometry ,Molecular biology ,medicine.anatomical_structure ,Spectrometry, Fluorescence ,Cancer cell ,Biophysics ,Molecular imaging ,Molecular probe ,DNA Probes ,Preclinical imaging - Abstract
Aptamers have emerged as promising molecular probes for in vivo cancer imaging, but the reported “always-on” aptamer probes remain problematic because of high background and limited contrast. To address this problem, we designed an activatable aptamer probe (AAP) targeting membrane proteins of living cancer cells and achieved contrast-enhanced cancer visualization inside mice. The AAP displayed a quenched fluorescence in its free state and underwent a conformational alteration upon binding to target cancer cells with an activated fluorescence. As proof of concept, in vitro analysis and in vivo imaging of CCRF-CEM cancer cells were performed by using the specific aptamer, sgc8, as a demonstration. It was confirmed that the AAP could be specifically activated by target cancer cells with a dramatic fluorescence enhancement and exhibit improved sensitivity for CCRF-CEM cell analysis with the cell number of 118 detected in 200 μl binding buffer. In vivo studies demonstrated that activated fluorescence signals were obviously achieved in the CCRF-CEM tumor sites in mice. Compared to always-on aptamer probes, the AAP could substantially minimize the background signal originating from nontarget tissues, thus resulting in significantly enhanced image contrast and shortened diagnosis time to 15 min. Furthermore, because of the specific affinity of sgc8 to target cancer cells, the AAP also showed desirable specificity in differentiating CCRF-CEM tumors from Ramos tumors and nontumor areas. The design concept can be widely adapted to other cancer cell-specific aptamer probes for in vivo molecular imaging of cancer.
- Published
- 2011
19. Activatable aptamer probe for contrast-enhanced in vivo cancer imaging based on cell membrane protein-triggered conformation alteration.
- Author
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Hui Shi, Xiaoxiao He, Kemin Wang, Xu Wu, Xiaosheng Ye, Qiuping Guo, Weihong Tan, Zhihe Qing, Xiaohai Yang, and Bing Zhou
- Subjects
CANCER diagnosis ,CELL membranes ,MOLECULAR probes ,MEMBRANE proteins ,CANCER cells ,FLUORESCENCE - Abstract
Aptamers have emerged as promising molecular probes for in vivo cancer imaging, but the reported "always-on" aptamer probes remain problematic because of high background and limited contrast. To address this problem, we designed an activatable aptamer probe (AAP) targeting membrane proteins of living cancer cells and achieved contrast-enhanced cancer visualization inside mice. The AAP displayed a quenched fluorescence in its free state and underwent a conformational alteration upon binding to target cancer cells with an activated fluorescence. As proof of concept, in vitro analysis and in vivo imaging of CCRF-CEM cancer cells were performed by using the specific aptamer, sgc8, as a demonstration. It was confirmed that the AAP could be specifically activated by target cancer cells with a dramatic fluorescence enhancement and exhibit improved sensitivity for CCRF-CEM cell analysis with the cell number of 118 detected in 200 μl binding buffer. In vivo studies demonstrated that activated fluorescence signals were obviously achieved in the CCRF-CEM tumor sites in mice. Compared to always-on aptamer probes, the AAP could substantially minimize the background signal originating from nontarget tissues, thus resulting in significantly enhanced image contrast and shortened diagnosis time to 15 min. Furthermore, because of the specific affinity of sgc8 to target cancer cells, the AAP also showed desirable specificity in differentiating CCRF-CEM tumors from Ramos tumors and nontumor areas. The design concept can be widely adapted to other cancer cell-specific aptamer probes for in vivo molecular imaging of cancer. [ABSTRACT FROM AUTHOR]
- Published
- 2011
- Full Text
- View/download PDF
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