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8 results on '"da Silva, Giarlã Cunha"'

1. Identification of novel small RNAs in extracellular vesicles produced by Actinobacillus pleuropneumoniae

Author

da Silva, Giarlã Cunha, primary, Rosa, Jéssica Nogueira, additional, Fontes, Patrícia Pereira, additional, de Castro, Alex Gazolla, additional, Barbosa, Éverton De Almeida Alves, additional, Clarindo, Wellington Ronildo, additional, Mantovani, Hilário Cuquetto, additional, Li, Yanwen, additional, Bossé, Janine Thérèse, additional, Langford, Paul Richard, additional, and Bazzolli, Denise Mara Soares, additional

Published
2023
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2. Identification of small RNAs associated with RNA chaperone Hfq reveals a new stress response regulator in Actinobacillus pleuropneumoniae

Author

da Silva, Giarlã Cunha, primary, Rossi, Ciro César, additional, Rosa, Jéssica Nogueira, additional, Sanches, Newton Moreno, additional, Cardoso, Daniela Lopes, additional, Li, Yanwen, additional, Witney, Adam A., additional, Gould, Kate A., additional, Fontes, Patrícia Pereira, additional, Callaghan, Anastasia J., additional, Bossé, Janine Thérèse, additional, Langford, Paul Richard, additional, and Bazzolli, Denise Mara Soares, additional

Published
2022
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3. Mobile Genetic Elements Drive Antimicrobial Resistance Gene Spread in Pasteurellaceae Species

Author

da Silva, Giarlã Cunha, Gonçalves, Osiel Silva, Rosa, Jéssica Nogueira, França, Kiara Campos, Bossé, Janine Thérèse, Santana, Mateus Ferreira, Langford, Paul Richard, Bazzolli, Denise Mara Soares, Biotechnology and Biological Sciences Research Council (BBSRC), and Biotechnology and Biological Sciences Research Council

Subjects
Microbiology (medical), one health, 0502 Environmental Science and Management, 0503 Soil Sciences, mobile DNA, bacterial resistance, genome evolution, gene transfer, Microbiology, QR1-502, 0605 Microbiology, Original Research
Abstract

Mobile genetic elements (MGEs) and antimicrobial resistance (AMR) drive important ecological relationships in microbial communities and pathogen-host interaction. In this study, we investigated the resistome-associated mobilome in 345 publicly available Pasteurellaceae genomes, a large family of Gram-negative bacteria including major human and animal pathogens. We generated a comprehensive dataset of the mobilome integrated into genomes, including 10,820 insertion sequences, 2,939 prophages, and 43 integrative and conjugative elements. Also, we assessed plasmid sequences of Pasteurellaceae. Our findings greatly expand the diversity of MGEs for the family, including a description of novel elements. We discovered that MGEs are comparable and dispersed across species and that they also co-occur in genomes, contributing to the family’s ecology via gene transfer. In addition, we investigated the impact of these elements in the dissemination and shaping of AMR genes. A total of 55 different AMR genes were mapped to 721 locations in the dataset. MGEs are linked with 77.6% of AMR genes discovered, indicating their important involvement in the acquisition and transmission of such genes. This study provides an uncharted view of the Pasteurellaceae by demonstrating the global distribution of resistance genes linked with MGEs.

Published
2022

4. Serovar-dependent differences in Hfq-regulated phenotypes inActinobacillus pleuropneumoniae

Author

Crispim, Josicelli Souza, primary, da Silva, Thyara Ferreira, additional, Sanches, Newton Moreno, additional, da Silva, Giarlã Cunha, additional, Pereira, Monalessa Fábia, additional, Rossi, Ciro César, additional, Li, Yanwen, additional, Terra, Vanessa Sofia, additional, Vohra, Prerna, additional, Wren, Brendan W, additional, Langford, Paul R, additional, Bossé, Janine T, additional, and Bazzolli, Denise Mara Soares, additional

Published
2020
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7. Evidence of Illegitimate Recombination Between Two Pasteurellaceae Plasmids Resulting in a Novel Multi-Resistance Replicon, pM3362MDR, in Actinobacillus pleuropneumoniae

Author

Li, Yinghui, primary, da Silva, Giarlã Cunha, additional, Li, Yanwen, additional, Rossi, Ciro C., additional, Fernandez Crespo, Roberto, additional, Williamson, Susanna M., additional, Langford, Paul R., additional, Bazzolli, Denise Mara Soares, additional, and Bossé, Janine T., additional

Published
2018
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8. Serovar-dependent differences in Hfq-regulated phenotypes in Actinobacillus pleuropneumoniae.

Author

Crispim, Josicelli Souza, da Silva, Thyara Ferreira, Sanches, Newton Moreno, da Silva, Giarlã Cunha, Pereira, Monalessa Fábia, Rossi, Ciro César, Li, Yanwen, Terra, Vanessa Sofia, Vohra, Prerna, Wren, Brendan W, Langford, Paul R, Bossé, Janine T, and Bazzolli, Denise Mara Soares

Subjects
ACTINOBACILLUS, ACTINOBACILLUS pleuropneumoniae, GREATER wax moth, PHENOTYPES, GENETIC regulation
Abstract

The RNA chaperone Hfq regulates diverse processes in numerous bacteria. In this study, we compared phenotypes (growth rate, adherence, response to different stress conditions and virulence in Galleria mellonella) of wild-type (WT) and isogenic hfq mutants of three serovars (1, 8 and 15) of the porcine pathogen Actinobacillus pleuropneumoniae. Similar growth in rich broth was seen for all strains except Ap1∆ hfq , which showed slightly reduced growth throughout the 24 h time course, and the complemented Ap8∆ hfq C mutant had a prolonged lag phase. Differences were seen between the three serovar WT strains regarding adherence, stress response and virulence in G. mellonella , and deletion of hfq affected some, but not all of these phenotypes, depending on serovar. Complementation by expression of cloned hfq from an endogenous promoter only restored some WT phenotypes, indicating that complex regulatory networks may be involved, and that levels of Hfq may be as important as presence/absence of the protein regarding its contribution to gene regulation. Our results support that Hfq is a pleiotropic global regulator in A. pleuropneumoniae , but serovar-related differences exist. These results highlight the importance of testing multiple strains/serovars within a given species when determining contributions of global regulators, such as Hfq, to expression of complex phenotypes. [ABSTRACT FROM AUTHOR]

Published
2020
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